Rorug05G0531100

zinc metallopeptidase EGY3, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000284
Physical Location & Seq
Forward (+)
71958611 .. 71965918
7308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0531100.1

Sequence Viewer

Length: 1728 bp
ATGTCGTATGTGCCTCCTCACCTGAGAAACTCAACCTCAACCACCACCACCACCACCACTACCACCGCCACCGCCAGAACCTCCTCCGGAACCGACGCTGATCGGAGCAAGCTCTCTTACTCCTCCTCCAACTCTCACTCCAACAACAACGACAAGTCGTCCGCTGCTTCATTCTCCTCGTTCTCGCACTCCAATGGCTCCCGGTGGAGCTCCGCCGCTCACTCGCCGGCCAACCGAGCTATCTCCCCGCCCGACGCCGTCGTCCCTCAGTGGAAGCCGTCGGAACGGGTCCTCCGAATGAAGCCGGAACTGATTGAGGAGGTGTGTTTGCGGCTTAATTTAGATGTTTCTGTTAGTCTGGACTCTGCTCCTCCCCCGGCACCAATTGAGTCTTTTACAGATATGTGTTTGCACCCTAGCATTATGAAGGATATAGCATTTCATGGATACACTATGCCAACACCAATTCAGGCTCAAGCCATGCCAGTTGCCCTCAGTGGAAGGGATTTATTAGGTTGCGCTGAAACTGGTAGTGGTAAAACTGCTGCATTTGCCATTCCTATGATACAGCATTGTTTGGCTCAACCTCCCGTTCAGCGTGGTGATGGACCACTAGCATTGGTACTGGCCCCAACTAGAGAGCTTGCACAACAAATAGAGGAAGAGGTCAAGGCTTTTAGTAAATCTCTTGAGTCCTTCAAAACTGCAATAGTTGTGGGTGGAACCAACATAGCTGAGCAGAGATCTGAGCTTCGAGCAGGAGTTAATATTGTGGTTGCTACTCCTGGAAGATTCATAGATCATTTACAGCAAGGGAACACTTCTGTGTCTAGAATTTCGTTTGTTGTTCTAGATGAAGCTGATAGAATGCTTGACATGGGTTTTGAACCACAGATAAGAGAGGTGATGCGGAACCTTTCTGAAAAGCATCAAACTTTGCTGTTTAGTGCAACTATGCCTGAAGAGATTGAAGGGTTAGCCCAGGAATATTTGACTAATCCAGTGCAAGTGAAGGTGGGAAAAGTGAGTAGCCCTACAGCAAATGTAACTCAGAGTTTGGAGAAGGTTCCTGACAGTGAGAAGACTGATCGGCTTCTGGGTTTGCTCGTAGAGGAGTCATCTCGAGCTGAAAGAGCTGGACATCCATTTCCCTTAACTATTGTATTTGTTGAAAGGAAGACACGCTGTGATGATGTAGCTGAAGCTTTGGTAGCACAAGGTTTGCATGCAGTTGCTCTTCATGGTGGACGTACTCAAACTGAAAGAGAGGCTGCTCTGCGTGAATTTAGAAAAGGCACTACCAACATCTTGGTTGCCACCGATGTTGCATCTCGTGGTTTGGACGTCTCAGGAGTTGCTCACGTTATCAATTTGGATCTTCCGAAGAGCATGGAAGATTATGTACACCGGATTGGAAGGACTGGAAGAGCGGGATCAATGGGACAAGCTACTTCCTTCTACACTGATCGTGATATGTTCCTTGTGGCGAATATAAAGAAAGCAATAGCTGATGTGGGATCTGGGAATGCTCTAGCTTTTGCTACGGGGAAGACTGCAAGAAGGAAAGAAAGAGAAGCAGCTGCTGCTCACAAGCAAGCTAGGGTTTCTCTATCCAAGTCTTCAAGTATGGGGCCCACATCAATAAACATTGAGGACAAGTATAAGTTCATGGTTTCTGAACCGGACAATCAAACAGAGGGTGCAGCAGATAGTGCTTGGGACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0006950 GO:0006979 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009636 GO:0009642 GO:0009644 GO:0010035 GO:0042221 GO:0042493 GO:0042542 GO:0046677 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

575

Amino Acids

62.04

Weight (kDa)

6.24

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 154 - 324 3.6e-53 DEAD/DEAH box helicase
ResIII PF04851 174 - 320 5.9e-09 Type III restriction enzyme, res subunit
Helicase_C PF00271 384 - 478 7.8e-30 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000284)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05675 AT1G05680
fragaria_vesca FvH4_2g03180 FvH4_2g03250 FvH4_2g03250 FvH4_2g03260 FvH4_2g03280 FvH4_2g03282 FvH4_2g03300 FvH4_2g17430 FvH4_3g07860
malus_domestica MD05G1106600.v1.1 MD10G1111000.v1.1 MD10G1111100.v1.1 MD10G1111300.v1.1 MD15G1305500.v1.1 MD15G1357700.v1.1 MD16G1086200.v1.1
prunus_persica Prupe.1G505100_v2.0.a1 Prupe.1G505200_v2.0.a1 Prupe.6G211600_v2.0.a1 Prupe.8G150600_v2.0.a1 Prupe.8G150700_v2.0.a1 Prupe.8G185900_v2.0.a1 Prupe.8G186000_v2.0.a1 Prupe.8G186100_v2.0.a1 Prupe.8G186200_v2.0.a1 Prupe.I000900_v2.0.a1 Prupe.I001000_v2.0.a1
pyrus_communis pycom10g09600 pycom10g09610 pycom10g09620 pycom15g31960
rosa_chinensis RchiOBHm_Chr6g0248151 RchiOBHm_Chr6g0248201 RchiOBHm_Chr6g0248231 RchiOBHm_Chr6g0248251 RchiOBHm_Chr6g0248261 RchiOBHm_Chr6g0248271 RchiOBHm_Chr6g0248321 RchiOBHm_Chr6g0248331 RchiOBHm_Chr6g0248381 RchiOBHm_Chr6g0248391 RchiOBHm_Chr6g0248401 RchiOBHm_Chr6g0248411 RchiOBHm_Chr6g0248421 RchiOBHm_Chr6g0282101 RchiOBHm_Chr6g0282111
rosa_laevigata RLG00000012908 RLG00000012910 RLG00000015155 RLG00000015156 RLG00000015157 RLG00000015158 RLG00000015159 RLG00000015162
rosa_multiflora Rmu_co8202196.1_g000001 Rmu_co8203792.1_g000001 Rmu_co8228231.1_g000001 Rmu_co8347131.1_g000001 Rmu_sc0000569.1_g000027 Rmu_sc0001700.1_g000052 Rmu_sc0002553.1_g000004 Rmu_sc0002553.1_g000005 Rmu_sc0004210.1_g000002 Rmu_sc0004295.1_g000009 Rmu_sc0004295.1_g000028 Rmu_sc0004295.1_g000034 Rmu_sc0005018.1_g000004 Rmu_sc0005018.1_g000007 Rmu_sc0015195.1_g000002 Rmu_sc0017247.1_g000001 Rmu_sc0041438.1_g000001
rosa_roxburghii Rroxscaffold_7G00186520 Rroxscaffold_7G00186550 Rroxscaffold_7G00213300 Rroxscaffold_7G00213340 Rroxscaffold_7G00213380 Rroxscaffold_7G00213400 Rroxscaffold_7G00213450
rosa_rugosa Rorug05G0530300 Rorug05G0531000 Rorug05G0531100 Rorug05G0552500 Rorug05G0552500 Rorug06G0145500
rosa_samantha Rh2AG004400 Rh6AG045600 Rh6AG045900 Rh6AG046200 Rh6AG046500 Rh6AG046700 Rh6AG258500 Rh6BG041200 Rh6BG041600 Rh6BG041700 Rh6BG041900 Rh6BG042000 Rh6BG042500 Rh6BG043000 Rh6BG043100 Rh6BG043200 Rh6BG043300 Rh6BG043400 Rh6BG261900 Rh6CG038700 Rh6CG038900 Rh6CG039100 Rh6CG039400 Rh6CG039700 Rh6CG039800 Rh6CG039900 Rh6CG040000 Rh6CG040100 Rh6CG260800
rosa_wichuraiana Rw6G003950 Rw6G003970 Rw6G003980 Rw6G004010 Rw6G004050 Rw6G004060 Rw6G004070 Rw6G004080 Rw6G022370 Rw6G022380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AatII GACGTC 1 cut(s) 1347
AccB1I GGYRCC 1 cut(s) 379
AccBSI CCGCTC 2 cut(s) 218, 1430
AccIII TCCGGA 1 cut(s) 86
AciI CCGC 9 cut(s) 66, 72, 162, 213, 216, 248, 331, 910, 1430
AclWI GGATC 3 cut(s) 1383, 1441, 1525
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 2 cut(s) 834, 1283
AcuI CTGAAG 2 cut(s) 981, 1221
AcyI GRCGYC 2 cut(s) 255, 1344
AdeI CACNNNGTG 1 cut(s) 1187
AfaI GTAC 3 cut(s) 624, 1252, 1404
AgsI TTSAA 5 cut(s) 700, 887, 971, 1172, 1623
AhdI GACNNNNNGTC 1 cut(s) 157
AjnI CCWGG 2 cut(s) 784, 981
AleI CACNNNNGTG 1 cut(s) 824
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 1351
AlwI GGATC 3 cut(s) 1383, 1441, 1525
AlwNI CAGNNNCTG 1 cut(s) 1583
Ama87I CYCGRG 1 cut(s) 1122
Aor13HI TCCGGA 1 cut(s) 86
AoxI GGCC 3 cut(s) 228, 627, 1631
ApaI GGGCCC 1 cut(s) 1635
ApeKI GCWGC 7 cut(s) 164, 545, 1271, 1577, 1580, 1583, 1703
ApoI RAATTY 2 cut(s) 834, 1283
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
AspLEI GCGC 1 cut(s) 521
AspS9I GGNCC 5 cut(s) 289, 608, 628, 1631, 1632
AsuC2I CCSGG 2 cut(s) 202, 377
AsuHPI GGTGA 3 cut(s) 11, 614, 916
AvaI CYCGRG 1 cut(s) 1122
AvaII GGWCC 2 cut(s) 289, 608
BaeGI GKGCMC 1 cut(s) 1635
BanI GGYRCC 1 cut(s) 379
BanII GRGCYC 2 cut(s) 212, 1635
BarI GAAGNNNNNNTAC 2 cut(s) 1386, 1418
BauI CACGAG 1 cut(s) 1332
BbsI GAAGAC 4 cut(s) 1088, 1184, 1556, 1611
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 7 cut(s) 151, 532, 1258, 1567, 1570, 1589, 1715
BccI CCATC 1 cut(s) 599
BceAI ACGGC 2 cut(s) 242, 262
BciT130I CCWGG 2 cut(s) 786, 983
BciVI GTATCC 1 cut(s) 440
BcnI CCSGG 2 cut(s) 202, 377
BcoDI GTCTC 1 cut(s) 1351
BfaI CTAG 7 cut(s) 417, 614, 636, 831, 851, 1532, 1599
BfmI CTRYAG 1 cut(s) 1035
BfuI GTATCC 1 cut(s) 440
BglII AGATCT 1 cut(s) 743
BisI GCNGC 9 cut(s) 165, 216, 332, 546, 1272, 1578, 1581, 1584, 1704
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 9 cut(s) 166, 217, 333, 547, 1273, 1579, 1582, 1585, 1705
Bme1390I CCNGG 4 cut(s) 202, 377, 786, 983
Bme18I GGWCC 2 cut(s) 289, 608
BmeRI GACNNNNNGTC 1 cut(s) 157
BmeT110I CYCGRG 1 cut(s) 1122
BmgT120I GGNCC 5 cut(s) 289, 608, 628, 1631, 1632
BmrFI CCNGG 4 cut(s) 202, 377, 786, 983
BmsI GCATC 3 cut(s) 897, 937, 1337
BpiI GAAGAC 4 cut(s) 1088, 1184, 1556, 1611
Bpu1102I GCTNAGC 1 cut(s) 735
BpuEI CTTGAG 2 cut(s) 459, 710
BpuMI CCSGG 2 cut(s) 202, 377
BsaHI GRCGYC 2 cut(s) 255, 1344
BsaJI CCNNGG 2 cut(s) 375, 981
BsaWI WCCGGW 3 cut(s) 86, 1407, 1681
BsaXI ACNNNNNCTCC 6 cut(s) 110, 122, 140, 152, 276, 306
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 5 cut(s) 485, 532, 630, 1001, 1426
BseAI TCCGGA 1 cut(s) 86
BseBI CCWGG 2 cut(s) 786, 983
BseDI CCNNGG 2 cut(s) 375, 981
BseGI GGATG 1 cut(s) 1141
BseMII CTCAG 7 cut(s) 14, 281, 508, 726, 738, 1064, 1362
BseNI ACTGG 5 cut(s) 485, 532, 630, 1001, 1426
BseRI GAGGAG 8 cut(s) 6, 73, 112, 115, 166, 332, 360, 1127
BseSI GKGCMC 1 cut(s) 1635
BseXI GCAGC 7 cut(s) 151, 532, 1258, 1567, 1570, 1589, 1715
BsgI GTGCAG 1 cut(s) 1722
BshFI GGCC 3 cut(s) 230, 629, 1633
BshNI GGYRCC 1 cut(s) 379
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 1122
BsiSI CCGG 7 cut(s) 87, 202, 227, 305, 377, 1408, 1682
BslFI GGGAC 2 cut(s) 248, 1455
BsmAI GTCTC 1 cut(s) 1351
BsmBI CGTCTC 1 cut(s) 1351
BsmFI GGGAC 2 cut(s) 248, 1455
BsmI GAATGC 2 cut(s) 873, 1531
BsnI GGCC 3 cut(s) 230, 629, 1633
BsoBI CYCGRG 1 cut(s) 1122
Bsp120I GGGCCC 1 cut(s) 1631
Bsp1286I GDGCHC 2 cut(s) 212, 1635
Bsp13I TCCGGA 1 cut(s) 86
Bsp1407I TGTACA 1 cut(s) 1402
Bsp143I GATC 8 cut(s) 100, 743, 799, 1087, 1375, 1433, 1465, 1517
Bsp1720I GCTNAGC 1 cut(s) 735
BspACI CCGC 9 cut(s) 66, 72, 162, 213, 216, 248, 331, 910, 1430
BspANI GGCC 3 cut(s) 230, 629, 1633
BspCNI CTCAG 7 cut(s) 15, 280, 507, 727, 739, 1063, 1361
BspEI TCCGGA 1 cut(s) 86
BspPI GGATC 3 cut(s) 1383, 1441, 1525
BspQI GCTCTTC 3 cut(s) 1242, 1379, 1420
BspT107I GGYRCC 1 cut(s) 379
BsrBI CCGCTC 2 cut(s) 218, 1430
BsrFI RCCGGY 1 cut(s) 226
BsrGI TGTACA 1 cut(s) 1402
BsrI ACTGG 5 cut(s) 485, 532, 630, 1001, 1426
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 2 cut(s) 375, 981
BssMI GATC 8 cut(s) 100, 743, 799, 1087, 1375, 1433, 1465, 1517
BssNI GRCGYC 2 cut(s) 255, 1344
BssSI CACGAG 1 cut(s) 1332
Bst2BI CACGAG 1 cut(s) 1332
Bst2UI CCWGG 2 cut(s) 786, 983
Bst4CI ACNGT 1 cut(s) 1076
Bst6I CTCTTC 5 cut(s) 657, 957, 1242, 1379, 1420
BstACI GRCGYC 2 cut(s) 255, 1344
BstAPI GCANNNNNTGC 2 cut(s) 1583, 1712
BstAUI TGTACA 1 cut(s) 1402
BstC8I GCNNGC 5 cut(s) 110, 228, 645, 1227, 1596
BstDEI CTNAG 7 cut(s) 23, 267, 494, 735, 747, 1050, 1348
BstF5I GGATG 1 cut(s) 1141
BstHHI GCGC 1 cut(s) 521
BstKTI GATC 8 cut(s) 103, 746, 802, 1090, 1378, 1436, 1468, 1520
BstMAI GTCTC 1 cut(s) 1351
BstMBI GATC 8 cut(s) 100, 743, 799, 1087, 1375, 1433, 1465, 1517
BstMWI GCNNNNNNNGC 6 cut(s) 236, 551, 1133, 1211, 1583, 1712
BstNI CCWGG 2 cut(s) 786, 983
BstNSI RCATGY 1 cut(s) 1229
BstSCI CCNGG 4 cut(s) 200, 375, 784, 981
BstSFI CTRYAG 1 cut(s) 1035
BstSLI GKGCMC 1 cut(s) 1635
BstV1I GCAGC 7 cut(s) 151, 532, 1258, 1567, 1570, 1589, 1715
BstV2I GAAGAC 4 cut(s) 1088, 1184, 1556, 1611
BstX2I RGATCY 3 cut(s) 743, 1375, 1517
BstXI CCANNNNNNTGG 1 cut(s) 1309
BstYI RGATCY 3 cut(s) 743, 1375, 1517
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 3 cut(s) 230, 629, 1633
BtsCI GGATG 1 cut(s) 1141
BtsIMutI CAGTG 5 cut(s) 275, 502, 1008, 1081, 1461
Cac8I GCNNGC 5 cut(s) 110, 228, 645, 1227, 1596
CaiI CAGNNNCTG 1 cut(s) 1583
CfoI GCGC 1 cut(s) 521
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 5 cut(s) 289, 608, 628, 1631, 1632
CseI GACGC 2 cut(s) 104, 263
Csp6I GTAC 3 cut(s) 623, 1251, 1403
CspCI CAANNNNNGTGG 6 cut(s) 600, 635, 696, 731, 1306, 1341
CviAII CATG 7 cut(s) 443, 481, 877, 1226, 1241, 1390, 1669
CviQI GTAC 3 cut(s) 623, 1251, 1403
DdeI CTNAG 7 cut(s) 23, 267, 494, 735, 747, 1050, 1348
DpnI GATC 8 cut(s) 102, 745, 801, 1089, 1377, 1435, 1467, 1519
DpnII GATC 8 cut(s) 100, 743, 799, 1087, 1375, 1433, 1465, 1517
DraIII CACNNNGTG 1 cut(s) 1187
DrdI GACNNNNNNGTC 1 cut(s) 260
DriI GACNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 5 cut(s) 657, 957, 1242, 1379, 1420
Eam1105I GACNNNNNGTC 1 cut(s) 157
EarI CTCTTC 5 cut(s) 657, 957, 1242, 1379, 1420
EciI GGCGGA 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 210
Eco24I GRGCYC 2 cut(s) 212, 1635
Eco47I GGWCC 2 cut(s) 289, 608
Eco53kI GAGCTC 1 cut(s) 210
Eco57I CTGAAG 2 cut(s) 981, 1221
Eco88I CYCGRG 1 cut(s) 1122
EcoICRI GAGCTC 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 289, 1631
EcoRII CCWGG 2 cut(s) 784, 981
EcoT38I GRGCYC 2 cut(s) 212, 1635
Esp3I CGTCTC 1 cut(s) 1351
FaeI CATG 7 cut(s) 446, 484, 880, 1229, 1244, 1393, 1672
FaqI GGGAC 2 cut(s) 248, 1455
FatI CATG 7 cut(s) 442, 480, 876, 1225, 1240, 1389, 1668
FauI CCCGC 2 cut(s) 255, 1423
Fnu4HI GCNGC 9 cut(s) 165, 216, 332, 546, 1272, 1578, 1581, 1584, 1704
FokI GGATG 1 cut(s) 1128
FriOI GRGCYC 2 cut(s) 212, 1635
Fsp4HI GCNGC 9 cut(s) 165, 216, 332, 546, 1272, 1578, 1581, 1584, 1704
FspBI CTAG 7 cut(s) 417, 614, 636, 831, 851, 1532, 1599
GlaI GCGC 1 cut(s) 520
GluI GCNGC 9 cut(s) 165, 216, 332, 546, 1272, 1578, 1581, 1584, 1704
HaeIII GGCC 3 cut(s) 230, 629, 1633
HapII CCGG 7 cut(s) 87, 202, 227, 305, 377, 1408, 1682
HgaI GACGC 2 cut(s) 104, 263
HhaI GCGC 1 cut(s) 521
Hin1I GRCGYC 2 cut(s) 255, 1344
Hin1II CATG 7 cut(s) 446, 484, 880, 1229, 1244, 1393, 1672
Hin6I GCGC 1 cut(s) 519
HinP1I GCGC 1 cut(s) 519
HindIII AAGCTT 1 cut(s) 1203
HinfI GANTC 5 cut(s) 362, 389, 692, 792, 1115
HpaII CCGG 7 cut(s) 87, 202, 227, 305, 377, 1408, 1682
HphI GGTGA 3 cut(s) 11, 614, 916
Hpy166II GTNNAC 2 cut(s) 1247, 1405
Hpy188I TCNGA 8 cut(s) 105, 283, 296, 748, 922, 1053, 1383, 1678
Hpy188III TCNNGA 9 cut(s) 87, 359, 689, 831, 851, 1070, 1122, 1350, 1469
Hpy8I GTNNAC 2 cut(s) 1247, 1405
Hpy99I CGWCG 4 cut(s) 98, 257, 263, 283
HpyAV CCTTC 9 cut(s) 421, 495, 706, 965, 1006, 1057, 1410, 1465, 1554
HpyCH4III ACNGT 1 cut(s) 1076
HpyCH4IV ACGT 3 cut(s) 1249, 1344, 1362
HpyF10VI GCNNNNNNNGC 6 cut(s) 236, 551, 1133, 1211, 1583, 1712
HpyF3I CTNAG 7 cut(s) 23, 267, 494, 735, 747, 1050, 1348
HpySE526I ACGT 3 cut(s) 1249, 1344, 1362
Hsp92I GRCGYC 2 cut(s) 255, 1344
Hsp92II CATG 7 cut(s) 446, 484, 880, 1229, 1244, 1393, 1672
HspAI GCGC 1 cut(s) 519
Kpn2I TCCGGA 1 cut(s) 86
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
Kzo9I GATC 8 cut(s) 100, 743, 799, 1087, 1375, 1433, 1465, 1517
LguI GCTCTTC 3 cut(s) 1242, 1379, 1420
LmnI GCTCC 5 cut(s) 105, 203, 207, 215, 373
Lsp1109I GCAGC 7 cut(s) 151, 532, 1258, 1567, 1570, 1589, 1715
LweI GCATC 3 cut(s) 897, 937, 1337
MaeI CTAG 7 cut(s) 417, 614, 636, 831, 851, 1532, 1599
MaeII ACGT 3 cut(s) 1249, 1344, 1362
MaeIII GTNAC 1 cut(s) 1045
MalI GATC 8 cut(s) 102, 745, 801, 1089, 1377, 1435, 1467, 1519
MbiI CCGCTC 2 cut(s) 218, 1430
MboI GATC 8 cut(s) 100, 743, 799, 1087, 1375, 1433, 1465, 1517
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 3 cut(s) 743, 1375, 1517
MhlI GDGCHC 2 cut(s) 212, 1635
MluCI AATT 6 cut(s) 337, 384, 465, 834, 1283, 1369
MlyI GAGTC 4 cut(s) 356, 398, 701, 1124
MmeI TCCRAC 3 cut(s) 153, 165, 261
MroI TCCGGA 1 cut(s) 86
MroNI GCCGGC 1 cut(s) 226
MseI TTAA 3 cut(s) 336, 765, 1154
MslI CAYNNNNRTG 3 cut(s) 192, 560, 824
MspA1I CMGCKG 2 cut(s) 164, 1580
MspI CCGG 7 cut(s) 87, 202, 227, 305, 377, 1408, 1682
MspR9I CCNGG 4 cut(s) 202, 377, 786, 983
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 2 cut(s) 873, 1531
MvaI CCWGG 2 cut(s) 786, 983
MwoI GCNNNNNNNGC 6 cut(s) 236, 551, 1133, 1211, 1583, 1712
NaeI GCCGGC 1 cut(s) 228
NciI CCSGG 2 cut(s) 202, 377
NdeII GATC 8 cut(s) 100, 743, 799, 1087, 1375, 1433, 1465, 1517
NgoMIV GCCGGC 1 cut(s) 226
NlaIII CATG 7 cut(s) 446, 484, 880, 1229, 1244, 1393, 1672
NspI RCATGY 1 cut(s) 1229
OliI CACNNNNGTG 1 cut(s) 824
PaeI GCATGC 1 cut(s) 1229
PaeR7I CTCGAG 1 cut(s) 1122
PciSI GCTCTTC 3 cut(s) 1242, 1379, 1420
PcsI WCGNNNNNNNCGW 1 cut(s) 292
PctI GAATGC 2 cut(s) 873, 1531
PdiI GCCGGC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 792
PflFI GACNNNGTC 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 784
PkrI GCNGC 9 cut(s) 166, 217, 333, 547, 1273, 1579, 1582, 1585, 1705
PleI GAGTC 4 cut(s) 356, 397, 700, 1123
PpsI GAGTC 4 cut(s) 356, 397, 700, 1123
PpuMI RGGWCCY 1 cut(s) 289
Psp124BI GAGCTC 1 cut(s) 212
Psp5II RGGWCCY 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 784, 981
PspGI CCWGG 2 cut(s) 784, 981
PspOMI GGGCCC 1 cut(s) 1631
PspPI GGNCC 5 cut(s) 289, 608, 628, 1631, 1632
PspPPI RGGWCCY 1 cut(s) 289
PstNI CAGNNNCTG 1 cut(s) 1583
PsuI RGATCY 3 cut(s) 743, 1375, 1517
PsyI GACNNNGTC 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 1580
RsaI GTAC 3 cut(s) 624, 1252, 1404
RsaNI GTAC 3 cut(s) 623, 1251, 1403
RseI CAYNNNNRTG 3 cut(s) 192, 560, 824
SacI GAGCTC 1 cut(s) 212
SapI GCTCTTC 3 cut(s) 1242, 1379, 1420
SaqAI TTAA 3 cut(s) 336, 765, 1154
SatI GCNGC 9 cut(s) 165, 216, 332, 546, 1272, 1578, 1581, 1584, 1704
Sau3AI GATC 8 cut(s) 100, 743, 799, 1087, 1375, 1433, 1465, 1517
Sau96I GGNCC 5 cut(s) 289, 608, 628, 1631, 1632
SchI GAGTC 4 cut(s) 356, 398, 701, 1124
ScrFI CCNGG 4 cut(s) 202, 377, 786, 983
SduI GDGCHC 2 cut(s) 212, 1635
SfaNI GCATC 3 cut(s) 897, 937, 1337
SfcI CTRYAG 1 cut(s) 1035
Sfr274I CTCGAG 1 cut(s) 1122
SinI GGWCC 2 cut(s) 289, 608
SlaI CTCGAG 1 cut(s) 1122
SmiMI CAYNNNNRTG 3 cut(s) 192, 560, 824
SmlI CTYRAG 3 cut(s) 474, 689, 1122
SmoI CTYRAG 3 cut(s) 474, 689, 1122
SphI GCATGC 1 cut(s) 1229
Sse9I AATT 6 cut(s) 337, 384, 465, 834, 1283, 1369
SsiI CCGC 9 cut(s) 66, 72, 162, 213, 216, 248, 331, 910, 1430
SspI AATATT 2 cut(s) 769, 989
SspMI CTAG 7 cut(s) 417, 614, 636, 831, 851, 1532, 1599
SstI GAGCTC 1 cut(s) 212
StyD4I CCNGG 4 cut(s) 200, 375, 784, 981
TaaI ACNGT 1 cut(s) 1076
TaiI ACGT 3 cut(s) 1252, 1347, 1365
TaqI TCGA 2 cut(s) 754, 1123
TasI AATT 6 cut(s) 337, 384, 465, 834, 1283, 1369
TatI WGTACW 1 cut(s) 1402
TauI GCSGC 2 cut(s) 218, 334
TfiI GAWTC 1 cut(s) 792
Tru1I TTAA 3 cut(s) 336, 765, 1154
Tru9I TTAA 3 cut(s) 336, 765, 1154
TscAI CASTG 5 cut(s) 275, 502, 1008, 1081, 1468
TseI GCWGC 7 cut(s) 164, 545, 1271, 1577, 1580, 1583, 1703
TspDTI ATGAA 8 cut(s) 159, 314, 431, 440, 784, 870, 1229, 1657
TspRI CASTG 5 cut(s) 275, 502, 1008, 1081, 1468
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 289, 608
XapI RAATTY 2 cut(s) 834, 1283
XbaI TCTAGA 2 cut(s) 830, 850
XceI RCATGY 1 cut(s) 1229
XhoI CTCGAG 1 cut(s) 1122
XspI CTAG 7 cut(s) 417, 614, 636, 831, 851, 1532, 1599
ZraI GACGTC 1 cut(s) 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.