FvH4_2g11460

glutamine amidotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012575
Physical Location & Seq
Forward (+)
10019749 .. 10023805
4057 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g11460.t1

Sequence Viewer

Length: 732 bp
ATGGGAGGGAAGAAATTTGGGGTTCTGCTGTGCGCAGAGGACACGGACTTTGTGAAGAAGATGTACGGAGGGTATTTCCAGGTGTTTCTGAAAATGCTGGCGGAGGAAGGCGAGACTTGGGACCTCTACCGCGTGGCCTGCGGCGAGTTTCCCGACGACGACGAGGTCGAGAGCTACGACGGGTTTGTCATCTCCGGGAGTAGCAGAGATGCTCATGGGAATGATGCTTGGATCTGCAGGCTACTCACCCTCTTGACCAGATTGGACTCCCTCAAGATCAAAGTCCTCGGCATTTGCTTCGGCCATCAGATATTAAGTCGTGCATTGGGTGGAAAATCAGGGAGAGCCATCACAGGGTGGGATATCGGAATCCGAACTGTCCACTTGTCATCATCTTGCAAGGTTCTCTCCTCTCTCAACATTCCAGCTTTTCTCTCCATCTATGAGATCCATCGAGATGAGGTGTGGGAGCTTCCACCAAAGGCTGAGCTAATTGCCTGGTCTGAAAAAACTGGAGTTGAGATGTTCAGGTATGGTGATCACATGATGGGCATCCAAGGCCACCCTGAATACACCAAAGACATTCTTCTCAACCTTATTGATCGTCTTGTCAAACTTAACTTCATCTTGGATTCTGAAACCGAGGAGTTGAAGTCAAAAGTGCTTGCCCGTGAACCAGATAGGGAGGCATGGAGAAGGTTGTGCTTAAACTTCCTCAAAGGTGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GATase

Protein Analysis

244

Amino Acids

27.5

Weight (kDa)

5.59

Isoelectric Point (pI)

30.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 24 - 192 1.9e-14 Glutamine amidotransferase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 164, 185
Acc16I TGCGCA 1 cut(s) 34
AccII CGCG 1 cut(s) 132
AciI CCGC 3 cut(s) 101, 130, 141
AclWI GGATC 2 cut(s) 239, 442
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 1 cut(s) 14
AdeI CACNNNGTG 1 cut(s) 357
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 354
AgsI TTSAA 1 cut(s) 652
AjnI CCWGG 2 cut(s) 78, 497
AluBI AGCT 4 cut(s) 174, 428, 472, 490
AluI AGCT 4 cut(s) 174, 428, 472, 490
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 2 cut(s) 239, 442
AoxI GGCC 3 cut(s) 135, 301, 559
ApoI RAATTY 1 cut(s) 14
ArsI GACNNNNNNTTYG 2 cut(s) 32, 64
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 121
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 238, 548
AvaII GGWCC 1 cut(s) 121
BarI GAAGNNNNNNTAC 2 cut(s) 47, 79
BccI CCATC 5 cut(s) 312, 356, 446, 459, 541
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciT130I CCWGG 2 cut(s) 80, 499
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 235
BisI GCNGC 1 cut(s) 142
BlpI GCTNAGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 143
Bme1390I CCNGG 3 cut(s) 80, 196, 499
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 3 cut(s) 80, 196, 499
BmsI GCATC 3 cut(s) 199, 214, 561
BpmI CTGGAG 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 196
BsaBI GATNNNNATC 1 cut(s) 551
BsaJI CCNNGG 3 cut(s) 286, 556, 642
BsaXI ACNNNNNCTCC 2 cut(s) 638, 668
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse1I ACTGG 1 cut(s) 517
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 2 cut(s) 80, 499
BseDI CCNNGG 3 cut(s) 286, 556, 642
BseGI GGATG 1 cut(s) 552
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 2 cut(s) 400, 659
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 137, 303, 561
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 354
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 3 cut(s) 137, 303, 561
Bsp143I GATC 5 cut(s) 231, 276, 447, 538, 601
Bsp1720I GCTNAGC 1 cut(s) 486
BspACI CCGC 3 cut(s) 101, 130, 141
BspANI GGCC 3 cut(s) 137, 303, 561
BspCNI CTCAG 1 cut(s) 478
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 122
BspMAI CTGCAG 1 cut(s) 239
BspPI GGATC 2 cut(s) 239, 442
BsrI ACTGG 1 cut(s) 517
BssECI CCNNGG 3 cut(s) 286, 556, 642
BssMI GATC 5 cut(s) 231, 276, 447, 538, 601
BssT1I CCWWGG 1 cut(s) 556
Bst2UI CCWGG 2 cut(s) 80, 499
Bst4CI ACNGT 1 cut(s) 379
BstC8I GCNNGC 4 cut(s) 99, 139, 239, 666
BstDEI CTNAG 1 cut(s) 486
BstF5I GGATG 1 cut(s) 552
BstFNI CGCG 1 cut(s) 132
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 5 cut(s) 234, 279, 450, 541, 604
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 5 cut(s) 231, 276, 447, 538, 601
BstMWI GCNNNNNNNGC 2 cut(s) 138, 558
BstNI CCWGG 2 cut(s) 80, 499
BstSCI CCNGG 3 cut(s) 78, 194, 497
BstSFI CTRYAG 1 cut(s) 235
BstUI CGCG 1 cut(s) 132
BstX2I RGATCY 2 cut(s) 231, 447
BstYI RGATCY 2 cut(s) 231, 447
BsuRI GGCC 3 cut(s) 137, 303, 561
BtsCI GGATG 1 cut(s) 552
Cac8I GCNNGC 4 cut(s) 99, 139, 239, 666
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 121
Csp6I GTAC 1 cut(s) 64
CviAII CATG 3 cut(s) 215, 544, 690
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 1 cut(s) 486
DpnI GATC 5 cut(s) 233, 278, 449, 540, 603
DpnII GATC 5 cut(s) 231, 276, 447, 538, 601
DraIII CACNNNGTG 1 cut(s) 357
DrdI GACNNNNNNGTC 2 cut(s) 164, 185
DseDI GACNNNNNNGTC 2 cut(s) 164, 185
EaeI YGGCCR 1 cut(s) 301
EciI GGCGGA 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 556
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 78, 497
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 556
FaeI CATG 3 cut(s) 218, 547, 693
FaiI YATR 6 cut(s) 216, 444, 534, 545, 691, 730
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 134
FatI CATG 3 cut(s) 214, 543, 689
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 1 cut(s) 539
Fsp4HI GCNGC 1 cut(s) 142
FspI TGCGCA 1 cut(s) 34
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 3 cut(s) 137, 303, 561
HapII CCGG 1 cut(s) 195
HhaI GCGC 1 cut(s) 35
Hin1II CATG 3 cut(s) 218, 547, 693
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 3 cut(s) 266, 369, 632
HpaII CCGG 1 cut(s) 195
HphI GGTGA 2 cut(s) 238, 548
Hpy166II GTNNAC 2 cut(s) 382, 674
Hpy188I TCNGA 6 cut(s) 90, 309, 368, 374, 505, 637
Hpy188III TCNNGA 5 cut(s) 152, 169, 253, 274, 455
Hpy8I GTNNAC 2 cut(s) 382, 674
Hpy99I CGWCG 4 cut(s) 158, 161, 164, 182
HpyAV CCTTC 2 cut(s) 101, 690
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4V TGCA 3 cut(s) 237, 323, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 558
HpyF3I CTNAG 1 cut(s) 486
Hsp92II CATG 3 cut(s) 218, 547, 693
HspAI GCGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 5 cut(s) 231, 276, 447, 538, 601
LmnI GCTCC 1 cut(s) 469
LweI GCATC 3 cut(s) 199, 214, 561
MalI GATC 5 cut(s) 233, 278, 449, 540, 603
MboI GATC 5 cut(s) 231, 276, 447, 538, 601
MboII GAAGA 4 cut(s) 22, 67, 70, 578
MflI RGATCY 2 cut(s) 231, 447
MluCI AATT 2 cut(s) 14, 492
MlyI GAGTC 1 cut(s) 260
MseI TTAA 3 cut(s) 314, 618, 707
MslI CAYNNNNRTG 2 cut(s) 219, 456
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 3 cut(s) 80, 196, 499
MvaI CCWGG 2 cut(s) 80, 499
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 2 cut(s) 138, 558
NciI CCSGG 1 cut(s) 196
NdeII GATC 5 cut(s) 231, 276, 447, 538, 601
NlaIII CATG 3 cut(s) 218, 547, 693
NlaIV GGNNCC 1 cut(s) 122
NmeAIII GCCGAG 1 cut(s) 267
NsbI TGCGCA 1 cut(s) 34
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 174
PfeI GAWTC 2 cut(s) 369, 632
PflFI GACNNNGTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 194
PkrI GCNGC 1 cut(s) 143
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 2 cut(s) 78, 497
PspGI CCWGG 2 cut(s) 78, 497
PspN4I GGNNCC 1 cut(s) 122
PspPI GGNCC 1 cut(s) 121
PspPPI RGGWCCY 1 cut(s) 121
PstI CTGCAG 1 cut(s) 239
PsuI RGATCY 2 cut(s) 231, 447
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
RseI CAYNNNNRTG 2 cut(s) 219, 456
SaqAI TTAA 3 cut(s) 314, 618, 707
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 5 cut(s) 231, 276, 447, 538, 601
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 3 cut(s) 80, 196, 499
SfaNI GCATC 3 cut(s) 199, 214, 561
SfcI CTRYAG 1 cut(s) 235
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 2 cut(s) 219, 456
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 2 cut(s) 14, 492
SsiI CCGC 3 cut(s) 101, 130, 141
StyD4I CCNGG 3 cut(s) 78, 194, 497
StyI CCWWGG 1 cut(s) 556
TaaI ACNGT 1 cut(s) 379
TaqI TCGA 2 cut(s) 168, 454
TasI AATT 2 cut(s) 14, 492
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 2 cut(s) 369, 632
Tru1I TTAA 3 cut(s) 314, 618, 707
Tru9I TTAA 3 cut(s) 314, 618, 707
TspDTI ATGAA 1 cut(s) 613
TspGWI ACGGA 2 cut(s) 59, 81
Tth111I GACNNNGTC 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 121
XapI RAATTY 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.