FvH4_2g14410
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016498
Physical Location & Seq
Forward (+)
12697873 .. 12701294
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14410.t1

Sequence Viewer

Length: 3273 bp
ATGTCAACTAATGCCATCACTATAATTCTCTTGTTTCTCAACATCTCTCTATTTCCTGCCATATCTGCTCTGAACCAAGAGGGTCTCTCCCTCCTTTCATGGCTTTCAACCTTCAATTCTTCTTCCTCAGCTGCCCATTTTTCTTCCTGGAATCCAACAGCCTACGATCCATGCAAATGGGATTATGTCAGATGTTCTGAAACAGGATTTGTTTCAGAAATCACAATAACCTCAATCAATATACCAACCAGATTCCCTATCCAGCTACTCACCTTTAATCATCTCACCACCCTTGTACTCTCAAATGCCAACCTCAGTGGAGAAATTCCAAAGGCAATTGGAAACTTGTCATCACTAACCACCCTAGATCTCAGCTTCAATGCTCTAAGAGGTTACATCCCACATGAGATGGGGAAATTATCACAACTGCAGTTGTTATCACTGAATTCTAATTCCTTGAATGGTGCAATTCCAAGAGAAATCGGAAACTGTTCTGAGCTTCAACAACTTGAACTGTACGATAACCAGTTATCTGGAACGATTCCTGCTGAAATAGGCCAGTTGTGGAATCTGGAAGTCTTCCGTGCAGGCGGGAATCCTGACATTCAAGGTGAAATCCCAATGCAGATATCAAACTGCAAGGGACTAACTTTCCTTGGCCTTGCAGCTACAGGAATCAGGGGACAAATTCCAAGAAGCATAGGAGAACTTAAGAATCTCCAAACTCTTTCGATTTATACAGCAAATCTCTCAGGTTACATCCCACCAGAAATAGGTAATTGTTCAGCCTTACAAGATTTGTTTCTGTATGATAACCAGCTTGCTGGTCAAATTCCTGTTCAACTTGGTCTATTGAACAAGCTAAGAAGAGTGCTACTGTGGAAGAACAATCTCAAAGGAAGCATCCCAGAAGCACTAGGGAACTGTTCAAAATTGAAAGTTATCGATTTCTCGTTGAATCTTCTGAGTGGTGAGGTTCCATTGTCTTTTGCAAACTTGGTGGCATTGGAGGAGCTTCTCATGTCTGAAAATCAGATATCAGGCAGCATACCGTCTTTACTTGGAAATATTTCCAGTTTAAAGCAACTGGAATTGGATAACAACAAATTTTCAGGTACAATTCCACCTGTCATTGGGCAATTAAGAGAACTTACTCTTTTCTTTGCCTGGAAGAATCAGCTCCAAGGAAGCATACCAACTGAGCTGGCCAACTGTAGGAAACTTCAAGCATTGGATCTTTCAGGCAATTTCCTTAGTGGGTCCATACCGAAATCTTTATTTGACCTCAAGAACTTAACGGAACTGTTGTTGATATCAAATGAATTCTCAGGACAAGTTCCAAGCAATATTGGCTACTGTACCAATTTGATCCGCTTACGCCTCGGATCAAACAACTTAACAGGTCAGATACCATCCGGAATTCGGCTCCTGCAGAGATTGAGCTTCCTAGAATTGTCAGGAAATCATTTTACCGGAATAATTCCTGCTGAAATAGGAGACTGCACCCGGTTAGAAATGGTTGATTTGCATGGAAATGAGCTCCAGGGCAGCATTCCTTCATCATTCCAATATCTTACTGGTCTTAATGTTTTAGACCTGTCCATGAACCAGATAGATGGTGAGATTCCTGAACCTTTTGGTAAGCTGAAATCCTTAAACAAACTGGTCATCAATGGAAACCACATTACTGGTTCCATTCCCAAGTCACTGAGCCTCTGTAGGGATTTGCAGTTGTTGGAAATAAGCAGAAACAAAATCACAGGCACTATCCCAGATGAGATTGGACGCTTGCAAGGGCTCGATATTCTCTTGAATCTGAGCTGGAATTCTCTGACAGGCCCCATTCCAGAAAGCTTCTCAAATTTCTCAAAACTTGCCAATATGGATATCTCCCACAATATGCTGACAGGAAGCTTGAAAGTACTTGGGGATCTGGACAACCTCGTTGCCCTGAATGTCTCATACAACGACTTTTCAGGTCTACTTCCCAATACTAAATTTTTCAAAGAGCTACCTCCATCTGCATTTGCCGGTAATCACAAGTTATGCATCAACGGAAATGAGTGCCAACTAAACAGAAACCTGCATGAGAAGAAATCCATCAGATATCTGATTATTTGTATCCTTCTGAGTGTAACTGCAACTATATTTGTTATGCTTGCTGGAATAGCTTTGTTTGTCAGAGTCTGGGGAATCACATTTGGGATGACAAACAGTAAAGAAGAAGAAGATCTGGTGTGGGATTTCACCCCATTTCAGAAGCTTGATTTTTCAGTTAATGACATTGTGACTAAGCTGTCAGACTCAAACATTGTTGGCAAGGGAGGCTCAGGAGTAGTTTATCGTGTAGAGACTCCAACAAGACATGTCATTGCAGTGAAGAAGTTATGGCCGATAAACAATGGGGAGCTTCCAGAAAGAGACTTTTTCTTTACAGAAGTTCATACTCTTGGATCGATAAGGCATAAGAATATAGTGAGGCTTCTGGGGTGTTGTAAAAATGGGAAAACCAGATTGCTTTTGTTTGATTTCATCAGTAATGGGAGCTTAGCTGGATTGCTCCATGAGAAGAGGGTGTTCTTGGACTGGGATACAAGGTACAAGATTGTACTGGGAGCAGCTCATGGCTTAGCTTATCTTCATCATGATTGCATTCCTCCAATCATTCATCGAGATATTAAGGCGAACAACATCTTGGTAGGGCCACAGTATGAATCTCTTCTTGCAGATTTTGGCCTTGCAAAGCTTTTGGGTTCTCCAGAATGCTGCAGAGCTTCCAACACAGTTGCAGGTTCTTATGGGTACATTGCTCCAGAATATGGATACAGTTTGAGGATCACAGAAAAGAGTGACGTGTATAGCTATGGAGTGGTGCTCCTAGAGGTCCTAACAGGGAAGGAACCAACAGATAACAAGATTCCTGAGGGTGAACATATTGTCACTTGGGTTAATAAGGAACTTGGAGAGAGAAACAAAGAATTTACGTCAATTGTTGATCATCAGCTAATACTGAGATCAGGGACAGAAATTCAAGAAATGTTTCAAGTTCTTGGGGTGGCTCTTCTCTGCGTTAATCCCTCCCCGGAGGAAAGGCCGACAATGAAAGATGTCACAGCAATGCTCAAGGAGATCAAGCATGAAAATGAGGATTGTGAAAACCCAAAATTTCTCGGCCAGGGAGTTGTGACAAACAGGAAAACTGCAGTTCACAGTTCTAGTTTCTCGAGGTCATCTGAACCTCTAATTGGTTCACCTTCTTGTTTTCCCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1091

Amino Acids

119.96

Weight (kDa)

6.02

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 66 9.7e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 92 - 195 6.3e-12 Leucine-rich repeat region
LRR_8 PF13855 94 - 153 3.2e-08 Leucine rich repeat
LRR_14 PF23598 209 - 314 4.7e-08 Leucine-rich repeat region
LRR_14 PF23598 282 - 461 8.1e-08 Leucine-rich repeat region
LRR_8 PF13855 287 - 346 7.7e-08 Leucine rich repeat
LRR_14 PF23598 474 - 556 9.5e-06 Leucine-rich repeat region
LRR_8 PF13855 503 - 562 9.1e-09 Leucine rich repeat
Pkinase PF00069 769 - 1038 1.6e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 770 - 1040 5.3e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016498)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g14410
malus_domestica MD05G1018000.v1.1
prunus_persica Prupe.8G022000_v2.0.a1
pyrus_communis pycom05g01070
rosa_chinensis RchiOBHm_Chr6g0276741
rosa_laevigata RLG00000013367
rosa_multiflora Rmu_sc0004481.1_g000026
rosa_roxburghii Rroxscaffold_7G00192010
rosa_rugosa Rorug06G0103400
rosa_samantha Rh6AG213800 Rh6BG218600 Rh6CG221600 Rh6DG211300
rosa_wichuraiana Rw6G018690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2296
Acc36I ACCTGC 2 cut(s) 2091, 2780
AccB7I CCANNNNNTGG 1 cut(s) 2819
AccI GTMKAC 1 cut(s) 1981
AccIII TCCGGA 1 cut(s) 1415
AciI CCGC 2 cut(s) 591, 1372
AclWI GGATC 7 cut(s) 161, 1242, 1363, 1393, 1938, 2461, 2843
AcoI YGGCCR 3 cut(s) 1206, 2390, 3172
AfaI GTAC 8 cut(s) 297, 518, 1117, 1360, 1923, 2600, 2610, 2804
AfiI CCNNNNNNNGG 7 cut(s) 773, 1133, 1215, 1422, 1720, 2819, 3245
AflII CTTAAG 1 cut(s) 710
AflIII ACRYGT 2 cut(s) 2365, 2853
AjiI CACGTC 1 cut(s) 2854
AjnI CCWGG 4 cut(s) 146, 1166, 1542, 3174
AjuI GAANNNNNNNTTGG 4 cut(s) 2678, 2710, 3228, 3260
Alw21I GWGCWC 2 cut(s) 1542, 2877
Alw26I GTCTC 5 cut(s) 89, 1491, 1963, 2345, 2415
AlwI GGATC 7 cut(s) 161, 1242, 1363, 1393, 1938, 2461, 2843
AlwNI CAGNNNCTG 1 cut(s) 2187
Ama87I CYCGRG 1 cut(s) 3223
Aor13HI TCCGGA 1 cut(s) 1415
AoxI GGCC 9 cut(s) 556, 658, 1206, 1837, 2390, 2702, 2734, 3092, 3172
ApeKI GCWGC 6 cut(s) 131, 665, 1044, 1548, 2618, 2766
Asp700I GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
AspS9I GGNCC 4 cut(s) 1260, 1838, 2702, 2884
AsuC2I CCSGG 2 cut(s) 1507, 3083
AsuHPI GGTGA 8 cut(s) 262, 277, 623, 983, 1631, 2239, 2939, 3243
AvaI CYCGRG 1 cut(s) 3223
AvaII GGWCC 2 cut(s) 1260, 2884
AxyI CCTNAGG 1 cut(s) 2922
BalI TGGCCA 1 cut(s) 1208
BanII GRGCYC 2 cut(s) 1542, 1800
BbsI GAAGAC 1 cut(s) 571
Bbv12I GWGCWC 2 cut(s) 1542, 2877
BbvCI CCTCAGC 1 cut(s) 127
BbvI GCAGC 6 cut(s) 118, 677, 1056, 1560, 2630, 2753
BccI CCATC 6 cut(s) 23, 403, 1420, 1610, 2026, 2108
BciT130I CCWGG 4 cut(s) 148, 1168, 1544, 3176
BciVI GTATCC 3 cut(s) 2132, 2584, 2816
BclI TGATCA 1 cut(s) 2995
BcnI CCSGG 2 cut(s) 1507, 3083
BcoDI GTCTC 5 cut(s) 89, 1491, 1963, 2345, 2415
BfaI CTAG 5 cut(s) 365, 917, 1448, 2879, 3216
BfmI CTRYAG 7 cut(s) 428, 669, 1213, 1430, 1717, 2767, 3201
BfrI CTTAAG 1 cut(s) 710
BfuAI ACCTGC 2 cut(s) 2091, 2780
BfuI GTATCC 3 cut(s) 2132, 2584, 2816
BglII AGATCT 2 cut(s) 367, 2230
BisI GCNGC 6 cut(s) 132, 666, 1045, 1549, 2619, 2767
BlpI GCTNAGC 2 cut(s) 2548, 2629
BlsI GCNGC 6 cut(s) 133, 667, 1046, 1550, 2620, 2768
BmcAI AGTACT 1 cut(s) 1923
Bme1390I CCNGG 6 cut(s) 148, 1168, 1507, 1544, 3083, 3176
Bme18I GGWCC 2 cut(s) 1260, 2884
BmeT110I CYCGRG 1 cut(s) 3223
BmgBI CACGTC 1 cut(s) 2854
BmgT120I GGNCC 4 cut(s) 1260, 1838, 2702, 2884
BmiI GGNNCC 6 cut(s) 978, 1261, 1427, 1693, 1840, 2901
BmrFI CCNGG 6 cut(s) 148, 1168, 1507, 1544, 3083, 3176
BmrI ACTGGG 2 cut(s) 2596, 2621
BmsI GCATC 2 cut(s) 912, 2058
BmuI ACTGGG 2 cut(s) 2596, 2621
BpiI GAAGAC 1 cut(s) 571
BplI GAGNNNNNCTC 4 cut(s) 71, 103, 2859, 2891
BpmI CTGGAG 3 cut(s) 1526, 2742, 2796
Bpu10I CCTNAGC 2 cut(s) 127, 2329
Bpu1102I GCTNAGC 2 cut(s) 2548, 2629
BpuEI CTTGAG 2 cut(s) 1271, 3107
BpuMI CCSGG 2 cut(s) 1507, 3083
Bsa29I ATCGAT 2 cut(s) 945, 2456
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 6 cut(s) 655, 1183, 1381, 1543, 3081, 3175
BsaWI WCCGGW 2 cut(s) 1415, 1472
Bsc4I CCNNNNNNNGG 7 cut(s) 773, 1133, 1215, 1422, 1720, 2819, 3245
Bse118I RCCGGY 1 cut(s) 2030
Bse1I ACTGG 9 cut(s) 526, 559, 1074, 1092, 1582, 1668, 1693, 2591, 2616
Bse21I CCTNAGG 1 cut(s) 2922
Bse3DI GCAATG 3 cut(s) 2370, 2805, 3123
BseAI TCCGGA 1 cut(s) 1415
BseBI CCWGG 4 cut(s) 148, 1168, 1544, 3176
BseCI ATCGAT 2 cut(s) 945, 2456
BseDI CCNNGG 6 cut(s) 655, 1183, 1381, 1543, 3081, 3175
BseGI GGATG 5 cut(s) 396, 759, 903, 1412, 2211
BseLI CCNNNNNNNGG 7 cut(s) 773, 1133, 1215, 1422, 1720, 2819, 3245
BseMI GCAATG 3 cut(s) 2370, 2805, 3123
BseNI ACTGG 9 cut(s) 526, 559, 1074, 1092, 1582, 1668, 1693, 2591, 2616
BseRI GAGGAG 1 cut(s) 1025
BseXI GCAGC 6 cut(s) 118, 677, 1056, 1560, 2630, 2753
BsgI GTGCAG 2 cut(s) 606, 1486
BshFI GGCC 9 cut(s) 558, 660, 1208, 1839, 2392, 2704, 2736, 3094, 3174
BshVI ATCGAT 2 cut(s) 945, 2456
BsiHKAI GWGCWC 2 cut(s) 1542, 2877
BsiHKCI CYCGRG 1 cut(s) 3223
BsiSI CCGG 5 cut(s) 1416, 1473, 1507, 2031, 3083
BslFI GGGAC 3 cut(s) 657, 696, 3034
BslI CCNNNNNNNGG 7 cut(s) 773, 1133, 1215, 1422, 1720, 2819, 3245
BsmAI GTCTC 5 cut(s) 89, 1491, 1963, 2345, 2415
BsmFI GGGAC 3 cut(s) 657, 696, 3034
BsmI GAATGC 3 cut(s) 1551, 2652, 2768
BsnI GGCC 9 cut(s) 558, 660, 1208, 1839, 2392, 2704, 2736, 3094, 3174
Bso31I GGTCTC 1 cut(s) 89
BsoBI CYCGRG 1 cut(s) 3223
Bsp1286I GDGCHC 3 cut(s) 1542, 1800, 2877
Bsp13I TCCGGA 1 cut(s) 1415
Bsp1720I GCTNAGC 2 cut(s) 2548, 2629
BspACI CCGC 2 cut(s) 591, 1372
BspANI GGCC 9 cut(s) 558, 660, 1208, 1839, 2392, 2704, 2736, 3094, 3174
BspDI ATCGAT 2 cut(s) 945, 2456
BspEI TCCGGA 1 cut(s) 1415
BspHI TCATGA 1 cut(s) 2644
BspLI GGNNCC 6 cut(s) 978, 1261, 1427, 1693, 1840, 2901
BspMAI CTGCAG 4 cut(s) 432, 1434, 2771, 3205
BspMI ACCTGC 2 cut(s) 2091, 2780
BspPI GGATC 7 cut(s) 161, 1242, 1363, 1393, 1938, 2461, 2843
BspQI GCTCTTC 1 cut(s) 3066
BspTI CTTAAG 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 89
BsrDI GCAATG 3 cut(s) 2370, 2805, 3123
BsrFI RCCGGY 1 cut(s) 2030
BsrI ACTGG 9 cut(s) 526, 559, 1074, 1092, 1582, 1668, 1693, 2591, 2616
BssAI RCCGGY 1 cut(s) 2030
BssECI CCNNGG 6 cut(s) 655, 1183, 1381, 1543, 3081, 3175
BssT1I CCWWGG 2 cut(s) 655, 1183
Bst2UI CCWGG 4 cut(s) 148, 1168, 1544, 3176
Bst6I CTCTTC 4 cut(s) 862, 2564, 2724, 3066
BstAFI CTTAAG 1 cut(s) 710
BstC8I GCNNGC 5 cut(s) 589, 822, 1206, 1790, 2160
BstF5I GGATG 5 cut(s) 396, 759, 903, 1412, 2211
BstMAI GTCTC 5 cut(s) 89, 1491, 1963, 2345, 2415
BstMWI GCNNNNNNNGC 4 cut(s) 65, 1350, 2168, 2325
BstNI CCWGG 4 cut(s) 148, 1168, 1544, 3176
BstNSI RCATGY 1 cut(s) 2369
BstSCI CCNGG 6 cut(s) 146, 1166, 1505, 1542, 3081, 3174
BstSFI CTRYAG 7 cut(s) 428, 669, 1213, 1430, 1717, 2767, 3201
BstV1I GCAGC 6 cut(s) 118, 677, 1056, 1560, 2630, 2753
BstV2I GAAGAC 1 cut(s) 571
BstX2I RGATCY 4 cut(s) 367, 1234, 1930, 2230
BstXI CCANNNNNNTGG 5 cut(s) 177, 533, 824, 1616, 1688
BstYI RGATCY 4 cut(s) 367, 1234, 1930, 2230
Bsu15I ATCGAT 2 cut(s) 945, 2456
Bsu36I CCTNAGG 1 cut(s) 2922
BsuI GTATCC 3 cut(s) 2132, 2584, 2816
BsuRI GGCC 9 cut(s) 558, 660, 1208, 1839, 2392, 2704, 2736, 3094, 3174
BsuTUI ATCGAT 2 cut(s) 945, 2456
BtrI CACGTC 1 cut(s) 2854
BtsCI GGATG 5 cut(s) 396, 759, 903, 1412, 2211
BtsI GCAGTG 1 cut(s) 2382
BtsIMutI CAGTG 4 cut(s) 322, 440, 1706, 2382
BveI ACCTGC 2 cut(s) 2091, 2780
Cac8I GCNNGC 5 cut(s) 589, 822, 1206, 1790, 2160
CaiI CAGNNNCTG 1 cut(s) 2187
CciI TCATGA 1 cut(s) 2644
Cfr10I RCCGGY 1 cut(s) 2030
Cfr13I GGNCC 4 cut(s) 1260, 1838, 2702, 2884
ClaI ATCGAT 2 cut(s) 945, 2456
CseI GACGC 1 cut(s) 1794
Csp6I GTAC 8 cut(s) 296, 517, 1116, 1359, 1922, 2599, 2609, 2803
CspCI CAANNNNNGTGG 4 cut(s) 298, 333, 981, 1016
CviQI GTAC 8 cut(s) 296, 517, 1116, 1359, 1922, 2599, 2609, 2803
DraI TTTAAA 1 cut(s) 1080
DrdI GACNNNNNNGTC 1 cut(s) 2296
DseDI GACNNNNNNGTC 1 cut(s) 2296
EaeI YGGCCR 3 cut(s) 1206, 2390, 3172
Eam1104I CTCTTC 4 cut(s) 862, 2564, 2724, 3066
EarI CTCTTC 4 cut(s) 862, 2564, 2724, 3066
Ecl136II GAGCTC 1 cut(s) 1540
Eco130I CCWWGG 2 cut(s) 655, 1183
Eco24I GRGCYC 2 cut(s) 1542, 1800
Eco31I GGTCTC 1 cut(s) 89
Eco32I GATATC 5 cut(s) 630, 1038, 1314, 1888, 2108
Eco47I GGWCC 2 cut(s) 1260, 2884
Eco53kI GAGCTC 1 cut(s) 1540
Eco81I CCTNAGG 1 cut(s) 2922
Eco88I CYCGRG 1 cut(s) 3223
EcoICRI GAGCTC 1 cut(s) 1540
EcoO109I RGGNCCY 2 cut(s) 1838, 2884
EcoRI GAATTC 4 cut(s) 445, 1322, 1419, 1825
EcoRII CCWGG 4 cut(s) 146, 1166, 1542, 3174
EcoRV GATATC 5 cut(s) 630, 1038, 1314, 1888, 2108
EcoT14I CCWWGG 2 cut(s) 655, 1183
EcoT22I ATGCAT 1 cut(s) 2051
EcoT38I GRGCYC 2 cut(s) 1542, 1800
ErhI CCWWGG 2 cut(s) 655, 1183
FaqI GGGAC 3 cut(s) 657, 696, 3034
FauI CCCGC 1 cut(s) 584
FbaI TGATCA 1 cut(s) 2995
FblI GTMKAC 1 cut(s) 1981
Fnu4HI GCNGC 6 cut(s) 132, 666, 1045, 1549, 2619, 2767
FokI GGATG 5 cut(s) 383, 746, 890, 1399, 2218
FriOI GRGCYC 2 cut(s) 1542, 1800
Fsp4HI GCNGC 6 cut(s) 132, 666, 1045, 1549, 2619, 2767
FspBI CTAG 5 cut(s) 365, 917, 1448, 2879, 3216
GluI GCNGC 6 cut(s) 132, 666, 1045, 1549, 2619, 2767
GsuI CTGGAG 3 cut(s) 1526, 2742, 2796
HaeIII GGCC 9 cut(s) 558, 660, 1208, 1839, 2392, 2704, 2736, 3094, 3174
HapII CCGG 5 cut(s) 1416, 1473, 1507, 2031, 3083
HgaI GACGC 1 cut(s) 1794
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 4 cut(s) 1852, 1912, 2261, 2744
HpaII CCGG 5 cut(s) 1416, 1473, 1507, 2031, 3083
HphI GGTGA 8 cut(s) 262, 277, 623, 983, 1631, 2239, 2939, 3243
Hpy166II GTNNAC 5 cut(s) 6, 1982, 2930, 3208, 3251
Hpy8I GTNNAC 5 cut(s) 6, 1982, 2930, 3208, 3251
HpyAV CCTTC 5 cut(s) 121, 1566, 2135, 2890, 3264
HpyCH4IV ACGT 2 cut(s) 2853, 2984
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 1350, 2168, 2325
HpySE526I ACGT 2 cut(s) 2853, 2984
Kpn2I TCCGGA 1 cut(s) 1415
Ksp22I TGATCA 1 cut(s) 2995
LguI GCTCTTC 1 cut(s) 3066
Lsp1109I GCAGC 6 cut(s) 118, 677, 1056, 1560, 2630, 2753
LweI GCATC 2 cut(s) 912, 2058
MaeI CTAG 5 cut(s) 365, 917, 1448, 2879, 3216
MaeII ACGT 2 cut(s) 2853, 2984
MaeIII GTNAC 9 cut(s) 392, 755, 1704, 2134, 2287, 2849, 2938, 3109, 3184
MfeI CAATTG 2 cut(s) 336, 2988
MflI RGATCY 4 cut(s) 367, 1234, 1930, 2230
MhlI GDGCHC 3 cut(s) 1542, 1800, 2877
MlsI TGGCCA 1 cut(s) 1208
MluNI TGGCCA 1 cut(s) 1208
MlyI GAGTC 3 cut(s) 2193, 2297, 2347
MmeI TCCRAC 4 cut(s) 179, 1716, 2381, 2802
Mox20I TGGCCA 1 cut(s) 1208
Mph1103I ATGCAT 1 cut(s) 2051
MroI TCCGGA 1 cut(s) 1415
MroXI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
MscI TGGCCA 1 cut(s) 1208
MslI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 3116, 3141
Msp20I TGGCCA 1 cut(s) 1208
MspA1I CMGCKG 1 cut(s) 131
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 5 cut(s) 1416, 1473, 1507, 2031, 3083
MspR9I CCNGG 6 cut(s) 148, 1168, 1507, 1544, 3083, 3176
MunI CAATTG 2 cut(s) 336, 2988
Mva1269I GAATGC 3 cut(s) 1551, 2652, 2768
MvaI CCWGG 4 cut(s) 148, 1168, 1544, 3176
MwoI GCNNNNNNNGC 4 cut(s) 65, 1350, 2168, 2325
NciI CCSGG 2 cut(s) 1507, 3083
NlaIV GGNNCC 6 cut(s) 978, 1261, 1427, 1693, 1840, 2901
NmeAIII GCCGAG 1 cut(s) 3150
NmuCI GTSAC 6 cut(s) 1704, 2287, 2849, 2938, 3109, 3184
NsiI ATGCAT 1 cut(s) 2051
NspI RCATGY 1 cut(s) 2369
PaeR7I CTCGAG 1 cut(s) 3223
PagI TCATGA 1 cut(s) 2644
PciI ACATGT 1 cut(s) 2365
PciSI GCTCTTC 1 cut(s) 3066
PctI GAATGC 3 cut(s) 1551, 2652, 2768
PdmI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
PflMI CCANNNNNTGG 1 cut(s) 2819
PfoI TCCNGGA 1 cut(s) 146
PkrI GCNGC 6 cut(s) 133, 667, 1046, 1550, 2620, 2768
PleI GAGTC 3 cut(s) 2192, 2297, 2347
PpsI GAGTC 3 cut(s) 2192, 2297, 2347
PpuMI RGGWCCY 1 cut(s) 2884
PscI ACATGT 1 cut(s) 2365
Psp124BI GAGCTC 1 cut(s) 1542
Psp5II RGGWCCY 1 cut(s) 2884
Psp6I CCWGG 4 cut(s) 146, 1166, 1542, 3174
PspGI CCWGG 4 cut(s) 146, 1166, 1542, 3174
PspN4I GGNNCC 6 cut(s) 978, 1261, 1427, 1693, 1840, 2901
PspPI GGNCC 4 cut(s) 1260, 1838, 2702, 2884
PspPPI RGGWCCY 1 cut(s) 2884
PstI CTGCAG 4 cut(s) 432, 1434, 2771, 3205
PstNI CAGNNNCTG 1 cut(s) 2187
PsuI RGATCY 4 cut(s) 367, 1234, 1930, 2230
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 8 cut(s) 297, 518, 1117, 1360, 1923, 2600, 2610, 2804
RsaNI GTAC 8 cut(s) 296, 517, 1116, 1359, 1922, 2599, 2609, 2803
RseI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 3116, 3141
SacI GAGCTC 1 cut(s) 1542
SapI GCTCTTC 1 cut(s) 3066
SatI GCNGC 6 cut(s) 132, 666, 1045, 1549, 2619, 2767
Sau96I GGNCC 4 cut(s) 1260, 1838, 2702, 2884
ScaI AGTACT 1 cut(s) 1923
SchI GAGTC 3 cut(s) 2193, 2297, 2347
ScrFI CCNGG 6 cut(s) 148, 1168, 1507, 1544, 3083, 3176
SduI GDGCHC 3 cut(s) 1542, 1800, 2877
SfaNI GCATC 2 cut(s) 912, 2058
SfcI CTRYAG 7 cut(s) 428, 669, 1213, 1430, 1717, 2767, 3201
Sfr274I CTCGAG 1 cut(s) 3223
SinI GGWCC 2 cut(s) 1260, 2884
SlaI CTCGAG 1 cut(s) 3223
SmiMI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 3116, 3141
SmlI CTYRAG 4 cut(s) 710, 1286, 3122, 3223
SmoI CTYRAG 4 cut(s) 710, 1286, 3122, 3223
SsiI CCGC 2 cut(s) 591, 1372
SspI AATATT 2 cut(s) 1069, 1348
SspMI CTAG 5 cut(s) 365, 917, 1448, 2879, 3216
SstI GAGCTC 1 cut(s) 1542
StyD4I CCNGG 6 cut(s) 146, 1166, 1505, 1542, 3081, 3174
StyI CCWWGG 2 cut(s) 655, 1183
TaiI ACGT 2 cut(s) 2856, 2987
TaqI TCGA 6 cut(s) 731, 945, 1800, 2456, 2671, 3224
TatI WGTACW 3 cut(s) 295, 1921, 2608
TscAI CASTG 4 cut(s) 322, 447, 1713, 2382
TseFI GTSAC 6 cut(s) 1704, 2287, 2849, 2938, 3109, 3184
TseI GCWGC 6 cut(s) 131, 665, 1044, 1548, 2618, 2766
Tsp45I GTSAC 6 cut(s) 1704, 2287, 2849, 2938, 3109, 3184
TspGWI ACGGA 3 cut(s) 572, 1313, 2070
TspRI CASTG 4 cut(s) 322, 447, 1713, 2382
Van91I CCANNNNNTGG 1 cut(s) 2819
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 1260, 2884
XceI RCATGY 1 cut(s) 2369
XcmI CCANNNNNNNNNTGG 1 cut(s) 1574
XhoI CTCGAG 1 cut(s) 3223
XmiI GTMKAC 1 cut(s) 1981
XmnI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
XspI CTAG 5 cut(s) 365, 917, 1448, 2879, 3216
ZrmI AGTACT 1 cut(s) 1923
Zsp2I ATGCAT 1 cut(s) 2051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.