pycom05g01070
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016498
Physical Location & Seq
Reverse (-)
1271512 .. 1274902
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01070.1

Sequence Viewer

Length: 3297 bp
ATGAAAAATAGCGTTGTTGCCAACCTCATCATGCCAAGCAATTCAATCACTATCATTCTTTTGCTTCTCAGCATCTTTCTGTTTCCTTCCATCTCTGCTCTGAACCAAGAGGGTATCTCCCTCCTTTCATGGCTTTCAACCTTCAATTCTTCTTCCTCTGCTGCCTTCTTCTCTTCATGGACTCCTGCTGATCAGGATCCATGCAAATGGGATTATATCAGATGTTCCAGTAACGGGTTTGTTTCCGAAATCAGAATCATCTCGATCCATATTCCTACTAGCTTCCCAATCCAGCTCCTCTCCTTCAACTATCTCACCACTCTTGTACTCTCAAATGCGAACCTCACTGGAGAAATCCCACGCTCGATTGGAAACTTGTCTTCGCTTATCACCCTTGATCTCAGCTTCAATGCTCTAACAGGAGATATTCCACAAGAGATAGGAAAGTTATCCAACCTGCAGTTGTTATCACTCAATTCAAATTCATTGCATGGTGCAATTCCCATAGAAATTGGAAACTGTTCAAAGCTTCAACAGCTTGAAGTGTATGACAACCATCTATCCGGAAAGGTTCCTGCAGAAATAGGCCAGTTGTTAGCTCTGGAAGTTTTTCGTGCAGGCGGGAATCCTGGAATTCATGGAGAAATTCCAATGCAAATATCAAAGTGCAAGAGTCTAACTATTCTTGGCCTTGCAGCTACCGGAATCACAGGGAAGATTCCAAGCAGTGTTGGAGAACTAAAGAATCTTCAAACTCTGTCGGTCTATACTGCAAACCTCACAGGTGACATCCCTCCTGAAATTGGTAATTGTTCAGCCTTACGAAATTTGTTTCTGTATGACAACCAGCTTTCTGGTCAAATTCCTGGTGAGCTTGGTCTGTTGAAAAGGCTTAAAAGCGTTCTGCTATGGAAGAACAATCTAAGTGGAAGCATTCCGAGTGCACTAGGGAACTGTTTGGATTTGATAGTTATCGATTTCTCAATTAATCATCTGAGTGGTGAAATTCCTCTCTCTTTTGCAAATTTAGTGGCACTGGAGGAGTTTCTCCTGTCTGAGAATCAGATATCAGGCAACATACCACCTTTATTCGGAAACTTTTCAAACTTAAAGCAACTTGAATTGGACAACAACAGATTTGTGGGCGTGATTCCACCTGTCATTGGGCAGCTAAAAGAACTTACTCTGTTCTATGCCTGGAATAATCAGCTCCATGGAAGCATACCAAATGAGCTGGCCAATTGTGACAAACTTCAAGCGCTGGATCTTTCACACAATCTCCTCACTGGGTCAGTTCCAAGTTCTCTCTTTGACCTCAAGAACTTAACTCAATTGTTGCTGATATCAAATCAATTTTTAGGAGAAGTTCCAAGGAATATCGGTAGCTGCACCGGCTTGGTCCGCTTAAGGCTGGGATCAAACAACTTCACAGGCCAAATTCCATCAGAACTTCGACTCCTGCATAGATTGAGCTTCCTTGAATTGTCAGAAAATCAATTTACTGGGGAAATTCCTCCTGATATCGGAAACTGCACTCAGTTAGAAATGGTTGACTTGCATGGAAATCAGCTGCAAGGCATGATTCCTTCGTCTTTGCAATTCCTTACTGGTCTTAATGTTTTAGACCTTTCCATGAACCAGATAGAAGGCACGATTCCTGAAGAGTTGGGCAAGCTAACATCCCTAAACAAACTGGTCATCAATGGAAATCGCATCACAGGTTCCATTCCTAAATCATTGGGCCTATGCAAGGATTTGCAGTTGTTAGATATGAGCAGCAACAATATCACTGGCACCATCCCAGATGAGATCGGACGCTTGCAAGAGCTAGATATTCTCTTGAATATGAGTTGGAATTCTCTGAGAGGCCCCATTCCAGAAAGCTTCTCAAATCTCTCAAAACTCGCCAATATGGACCTCTCCCATAATTTTTTGACAGGAAGCCTCAGAGTACTGGGTAGTCTTGACAACCTCGTGTCCCTAAATGTCTCACACAATGACTTCTCAGGTCCACTTCCCAATACTAATTTCTTCAAAGACATTCCACCAACTGCGTTTGCTGGTAACCCGAAGCTGTGTATCAACAGAAATCAGTGCCACTTGAATGACAACCTGCACGACAAGAAATCAATCAGATATCTACTTGTTTGCGTTCTTCTTAGCGTGACTGCAACCATCATGGTCATGCTCACTGGAATATTTTTATTTGTCAGAATTTGTGGAATCACATTTGGGATGCACAGTGAACCAGAAAATGATCTGGTGTGGGCTTTCACCCCATTTCAAAAGCTTAATTTTTCTGTTAATGATATTGTGACTGGGCTATCAGAATCAAACGTTGTGGGAAAGGGTTGCTCGGGAATGGTTTATCGTGTGGAGACTCCAACGAAGCAGGTCATTGCAGTGAAGAAGTTATGGCCAGTAAATAATGGCGAGCTCCCAGAGAGGGACTTGTTTACGACAGAAGTTCATACTCTTGGATCAATTAGGCATAAAAACATAGTGAGGCTTCTGGGGTGTTGTAACAATGGAAAAACCAGACTTCTTTTGTTCGATTACATCGCGAATGGGAGTTTGGCGAGCTTGCTACACGAGAAGAGGCTGTTCTTGGATTGGGATGCAAGGTACAAGATTGTACTGGGAGCAGCTCAGGGTTTAGCTTATCTCCATCACGATTGTATACCTCCAATAGTTCATCGAGATATCAAGGCTAACAACATCTTGGTTGGACCACAGTATGAATCTTTTATAGCAGATTTTGGCATTGCAAAGCTTTTGAATTCTCCAGAATGCTGCAAAGCCTCCAACACAGTTGCAGGTTCTTATGGCTACATTGCTCCAGAATATGGATACAGTTTGAGGATCACAGAGAAGAGCGATGTGTATAGCTATGGAGTGGTCCTCCTGGAGGTCCTGACCGGGAAGGAACCAATGGATAACAAGATCCCGGAAGGTGCCCACATTGTCACTTGGGTGAATACGGAACTTAGAGTAAGGAAAAGAGATTTCACATCAGTTCTAGACCAGCAGCTAATTTTGCTATCAGGGACACAAACCCAAGAGATGCTTCAAGTGCTTGGGGTGGCTCTTCTCTGTGTGAATCCTTGCCCGGAGGAAAGGCCAACTATGAAAGATGTAACAGCAATGCTCAAGGAGATTCGACATGAGAACGAGGATTGTGAAAAACCAGATTTTCTTGGCAAAGGAGTTGCTACAAACCGAAACGCTACAGTTCACAGTTCTAGTTTCTCCAGGTCATCTGAACCTCTAATTGGATCACCTTCTTGTTCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1099

Amino Acids

120.2

Weight (kDa)

5.74

Isoelectric Point (pI)

31.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 76 1.5e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 105 - 163 1.3e-06 Leucine rich repeat
LRR_14 PF23598 106 - 205 7.6e-10 Leucine-rich repeat region
LRR_14 PF23598 214 - 320 8.8e-09 Leucine-rich repeat region
LRR_14 PF23598 483 - 566 7.6e-07 Leucine-rich repeat region
LRR_8 PF13855 513 - 572 1.8e-08 Leucine rich repeat
Pkinase PF00069 778 - 1047 5.3e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 778 - 1048 8.7e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016498)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g14410
malus_domestica MD05G1018000.v1.1
prunus_persica Prupe.8G022000_v2.0.a1
pyrus_communis pycom05g01070
rosa_chinensis RchiOBHm_Chr6g0276741
rosa_laevigata RLG00000013367
rosa_multiflora Rmu_sc0004481.1_g000026
rosa_roxburghii Rroxscaffold_7G00192010
rosa_rugosa Rorug06G0103400
rosa_samantha Rh6AG213800 Rh6BG218600 Rh6CG221600 Rh6DG211300
rosa_wichuraiana Rw6G018690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 465, 2121, 2383, 2807
AccB1I GGYRCC 2 cut(s) 1793, 2954
AccB7I CCANNNNNTGG 1 cut(s) 2846
AccI GTMKAC 1 cut(s) 2680
AccII CGCG 1 cut(s) 2564
AccIII TCCGGA 1 cut(s) 563
AciI CCGC 2 cut(s) 621, 1402
AclI AACGTT 1 cut(s) 2337
AclWI GGATC 9 cut(s) 191, 204, 259, 1272, 1423, 2488, 2870, 2938, 3283
AcoI YGGCCR 2 cut(s) 1236, 2417
AcuI CTGAAG 1 cut(s) 1680
AfaI GTAC 4 cut(s) 327, 1953, 2627, 2637
AfeI AGCGCT 1 cut(s) 1260
AflII CTTAAG 1 cut(s) 1405
AjnI CCWGG 5 cut(s) 628, 865, 1196, 2904, 3251
AjuI GAANNNNNNNTTGG 8 cut(s) 2012, 2044, 2558, 2590, 3051, 3083, 3255, 3287
AleI CACNNNNGTG 1 cut(s) 2972
AloI GAACNNNNNNTCC 2 cut(s) 1440, 1472
Alw21I GWGCWC 2 cut(s) 946, 2439
Alw26I GTCTC 2 cut(s) 1993, 2372
Alw44I GTGCAC 1 cut(s) 942
AlwI GGATC 9 cut(s) 191, 204, 259, 1272, 1423, 2488, 2870, 2938, 3283
Ama87I CYCGRG 1 cut(s) 2356
Aor13HI TCCGGA 1 cut(s) 563
Aor51HI AGCGCT 1 cut(s) 1260
AoxI GGCC 8 cut(s) 586, 688, 1236, 1432, 1741, 1867, 2417, 3119
ApaLI GTGCAC 1 cut(s) 942
ApeKI GCWGC 9 cut(s) 161, 695, 1168, 1386, 1570, 1776, 2645, 2793, 3028
AseI ATTAAT 1 cut(s) 987
Asp700I GAANNNNTTC 3 cut(s) 520, 609, 1099
AspLEI GCGC 1 cut(s) 1261
AspS9I GGNCC 8 cut(s) 1399, 1741, 1868, 1915, 2009, 2729, 2899, 2911
AsuC2I CCSGG 3 cut(s) 2920, 2948, 3110
AsuHPI GGTGA 8 cut(s) 307, 382, 797, 881, 1013, 2266, 2986, 3270
AvaI CYCGRG 1 cut(s) 2356
AvaII GGWCC 6 cut(s) 1399, 1915, 2009, 2729, 2899, 2911
BaeGI GKGCMC 2 cut(s) 946, 2959
BalI TGGCCA 2 cut(s) 1238, 2419
BamHI GGATCC 1 cut(s) 196
BanI GGYRCC 2 cut(s) 1793, 2954
BanII GRGCYC 1 cut(s) 2439
BauI CACGAG 2 cut(s) 1973, 2591
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 946, 2439
BbvI GCAGC 9 cut(s) 148, 707, 1180, 1373, 1557, 1788, 2657, 2780, 3040
BccI CCATC 6 cut(s) 98, 564, 1450, 1805, 2183, 2676
BciT130I CCWGG 5 cut(s) 630, 867, 1198, 2906, 3253
BciVI GTATCC 1 cut(s) 2843
BclI TGATCA 1 cut(s) 190
BcnI CCSGG 3 cut(s) 2920, 2948, 3110
BcoDI GTCTC 2 cut(s) 1993, 2372
BfaI CTAG 5 cut(s) 279, 947, 1829, 3020, 3243
BfmI CTRYAG 3 cut(s) 458, 576, 3228
BfoI RGCGCY 1 cut(s) 1262
BfrI CTTAAG 1 cut(s) 1405
BfuAI ACCTGC 4 cut(s) 465, 2121, 2383, 2807
BfuI GTATCC 1 cut(s) 2843
BisI GCNGC 9 cut(s) 162, 696, 1169, 1387, 1571, 1777, 2646, 2794, 3029
BlsI GCNGC 9 cut(s) 163, 697, 1170, 1388, 1572, 1778, 2647, 2795, 3030
BmcAI AGTACT 1 cut(s) 1953
Bme1390I CCNGG 8 cut(s) 630, 867, 1198, 2906, 2920, 2948, 3110, 3253
Bme18I GGWCC 6 cut(s) 1399, 1915, 2009, 2729, 2899, 2911
BmeT110I CYCGRG 1 cut(s) 2356
BmgT120I GGNCC 8 cut(s) 1399, 1741, 1868, 1915, 2009, 2729, 2899, 2911
BmiI GGNNCC 7 cut(s) 198, 573, 1723, 1795, 1870, 2928, 2956
BmrFI CCNGG 8 cut(s) 630, 867, 1198, 2906, 2920, 2948, 3110, 3253
BmrI ACTGGG 5 cut(s) 1296, 1512, 1964, 2328, 2648
BmsI GCATC 5 cut(s) 81, 1722, 2226, 2608, 3054
BmuI ACTGGG 5 cut(s) 1296, 1512, 1964, 2328, 2648
BpiI GAAGAC 1 cut(s) 372
BplI GAGNNNNNCTC 4 cut(s) 101, 133, 2886, 2918
BpmI CTGGAG 6 cut(s) 369, 1058, 2769, 2823, 2927, 3235
Bpu10I CCTNAGC 1 cut(s) 2649
BpuEI CTTGAG 2 cut(s) 1301, 3134
BpuMI CCSGG 3 cut(s) 2920, 2948, 3110
Bsa29I ATCGAT 1 cut(s) 975
BsaBI GATNNNNATC 2 cut(s) 195, 971
BsaJI CCNNGG 2 cut(s) 1213, 1370
BsaWI WCCGGW 2 cut(s) 563, 701
BsaXI ACNNNNNCTCC 4 cut(s) 1263, 1293, 1440, 1470
Bse118I RCCGGY 1 cut(s) 1391
Bse3DI GCAATG 5 cut(s) 485, 2397, 2763, 2832, 3150
Bse8I GATNNNNATC 2 cut(s) 195, 971
BseAI TCCGGA 1 cut(s) 563
BseBI CCWGG 5 cut(s) 630, 867, 1198, 2906, 3253
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 2 cut(s) 1213, 1370
BseGI GGATG 5 cut(s) 789, 1679, 1797, 2241, 2623
BseJI GATNNNNATC 2 cut(s) 195, 971
BseMI GCAATG 5 cut(s) 485, 2397, 2763, 2832, 3150
BseMII CTCAG 9 cut(s) 82, 415, 986, 1047, 1550, 1853, 1960, 2019, 2663
BseRI GAGGAG 3 cut(s) 287, 1055, 1271
BseSI GKGCMC 2 cut(s) 946, 2959
BseXI GCAGC 9 cut(s) 148, 707, 1180, 1373, 1557, 1788, 2657, 2780, 3040
BseYI CCCAGC 1 cut(s) 1411
BsgI GTGCAG 4 cut(s) 636, 1372, 1516, 2099
Bsh1236I CGCG 1 cut(s) 2564
BshFI GGCC 8 cut(s) 588, 690, 1238, 1434, 1743, 1869, 2419, 3121
BshNI GGYRCC 2 cut(s) 1793, 2954
BshVI ATCGAT 1 cut(s) 975
BsiHKAI GWGCWC 2 cut(s) 946, 2439
BsiHKCI CYCGRG 1 cut(s) 2356
BsiSI CCGG 6 cut(s) 564, 702, 1392, 2919, 2948, 3110
BslFI GGGAC 3 cut(s) 1963, 2462, 3061
BsmAI GTCTC 2 cut(s) 1993, 2372
BsmFI GGGAC 3 cut(s) 1963, 2462, 3061
BsmI GAATGC 2 cut(s) 933, 2795
BsnI GGCC 8 cut(s) 588, 690, 1238, 1434, 1743, 1869, 2419, 3121
BsoBI CYCGRG 1 cut(s) 2356
Bsp1286I GDGCHC 3 cut(s) 946, 2439, 2959
Bsp13I TCCGGA 1 cut(s) 563
Bsp19I CCATGG 1 cut(s) 1213
Bsp68I TCGCGA 1 cut(s) 2564
BspACI CCGC 2 cut(s) 621, 1402
BspANI GGCC 8 cut(s) 588, 690, 1238, 1434, 1743, 1869, 2419, 3121
BspCNI CTCAG 9 cut(s) 81, 414, 987, 1048, 1549, 1854, 1959, 2018, 2662
BspDI ATCGAT 1 cut(s) 975
BspEI TCCGGA 1 cut(s) 563
BspFNI CGCG 1 cut(s) 2564
BspLI GGNNCC 7 cut(s) 198, 573, 1723, 1795, 1870, 2928, 2956
BspMAI CTGCAG 2 cut(s) 462, 580
BspMI ACCTGC 4 cut(s) 465, 2121, 2383, 2807
BspPI GGATC 9 cut(s) 191, 204, 259, 1272, 1423, 2488, 2870, 2938, 3283
BspQI GCTCTTC 2 cut(s) 2867, 3093
BspT107I GGYRCC 2 cut(s) 1793, 2954
BspTI CTTAAG 1 cut(s) 1405
BsrDI GCAATG 5 cut(s) 485, 2397, 2763, 2832, 3150
BsrFI RCCGGY 1 cut(s) 1391
BssAI RCCGGY 1 cut(s) 1391
BssECI CCNNGG 2 cut(s) 1213, 1370
BssNAI GTATAC 1 cut(s) 2681
BssSI CACGAG 2 cut(s) 1973, 2591
BssT1I CCWWGG 2 cut(s) 1213, 1370
Bst1107I GTATAC 1 cut(s) 2681
Bst2BI CACGAG 2 cut(s) 1973, 2591
Bst2UI CCWGG 5 cut(s) 630, 867, 1198, 2906, 3253
Bst4CI ACNGT 8 cut(s) 521, 956, 2243, 2736, 2812, 2855, 3232, 3239
Bst6I CTCTTC 5 cut(s) 178, 1656, 2591, 2867, 3093
BstAFI CTTAAG 1 cut(s) 1405
BstC8I GCNNGC 7 cut(s) 619, 1236, 1673, 1820, 2435, 2581, 2585
BstDSI CCRYGG 1 cut(s) 1213
BstEII GGTNACC 1 cut(s) 2063
BstENI CCTNNNNNAGG 2 cut(s) 1748, 2906
BstF5I GGATG 5 cut(s) 789, 1679, 1797, 2241, 2623
BstFNI CGCG 1 cut(s) 2564
BstH2I RGCGCY 1 cut(s) 1262
BstHHI GCGC 1 cut(s) 1261
BstMAI GTCTC 2 cut(s) 1993, 2372
BstMWI GCNNNNNNNGC 5 cut(s) 535, 1392, 1401, 3037, 3073
BstNI CCWGG 5 cut(s) 630, 867, 1198, 2906, 3253
BstPI GGTNACC 1 cut(s) 2063
BstSCI CCNGG 8 cut(s) 628, 865, 1196, 2904, 2918, 2946, 3108, 3251
BstSFI CTRYAG 3 cut(s) 458, 576, 3228
BstSLI GKGCMC 2 cut(s) 946, 2959
BstUI CGCG 1 cut(s) 2564
BstV1I GCAGC 9 cut(s) 148, 707, 1180, 1373, 1557, 1788, 2657, 2780, 3040
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 3 cut(s) 196, 1264, 2943
BstXI CCANNNNNNTGG 2 cut(s) 207, 854
BstYI RGATCY 3 cut(s) 196, 1264, 2943
BstZ17I GTATAC 1 cut(s) 2681
Bsu15I ATCGAT 1 cut(s) 975
BsuI GTATCC 1 cut(s) 2843
BsuRI GGCC 8 cut(s) 588, 690, 1238, 1434, 1743, 1869, 2419, 3121
BsuTUI ATCGAT 1 cut(s) 975
BtgI CCRYGG 1 cut(s) 1213
BtgZI GCGATG 2 cut(s) 2545, 2892
BtsCI GGATG 5 cut(s) 789, 1679, 1797, 2241, 2623
BtsI GCAGTG 2 cut(s) 733, 2409
BtsIMutI CAGTG 9 cut(s) 345, 733, 1034, 1284, 1788, 2099, 2190, 2248, 2409
BtuMI TCGCGA 1 cut(s) 2564
BveI ACCTGC 4 cut(s) 465, 2121, 2383, 2807
Cac8I GCNNGC 7 cut(s) 619, 1236, 1673, 1820, 2435, 2581, 2585
CfoI GCGC 1 cut(s) 1261
Cfr10I RCCGGY 1 cut(s) 1391
Cfr13I GGNCC 8 cut(s) 1399, 1741, 1868, 1915, 2009, 2729, 2899, 2911
ClaI ATCGAT 1 cut(s) 975
CseI GACGC 1 cut(s) 1824
Csp6I GTAC 4 cut(s) 326, 1952, 2626, 2636
CspCI CAANNNNNGTGG 6 cut(s) 907, 942, 1011, 1046, 2322, 2357
CviQI GTAC 4 cut(s) 326, 1952, 2626, 2636
EaeI YGGCCR 2 cut(s) 1236, 2417
Eam1104I CTCTTC 5 cut(s) 178, 1656, 2591, 2867, 3093
EarI CTCTTC 5 cut(s) 178, 1656, 2591, 2867, 3093
Ecl136II GAGCTC 1 cut(s) 2437
Eco130I CCWWGG 2 cut(s) 1213, 1370
Eco24I GRGCYC 1 cut(s) 2439
Eco32I GATATC 5 cut(s) 1068, 1344, 1522, 2138, 2704
Eco47I GGWCC 6 cut(s) 1399, 1915, 2009, 2729, 2899, 2911
Eco47III AGCGCT 1 cut(s) 1260
Eco53kI GAGCTC 1 cut(s) 2437
Eco57I CTGAAG 1 cut(s) 1680
Eco88I CYCGRG 1 cut(s) 2356
Eco91I GGTNACC 1 cut(s) 2063
EcoICRI GAGCTC 1 cut(s) 2437
EcoNI CCTNNNNNAGG 2 cut(s) 1748, 2906
EcoO109I RGGNCCY 2 cut(s) 1868, 2911
EcoO65I GGTNACC 1 cut(s) 2063
EcoRI GAATTC 3 cut(s) 633, 1855, 2779
EcoRII CCWGG 5 cut(s) 628, 865, 1196, 2904, 3251
EcoRV GATATC 5 cut(s) 1068, 1344, 1522, 2138, 2704
EcoT14I CCWWGG 2 cut(s) 1213, 1370
EcoT38I GRGCYC 1 cut(s) 2439
ErhI CCWWGG 2 cut(s) 1213, 1370
FalI AAGNNNNNCTT 2 cut(s) 3051, 3083
FaqI GGGAC 3 cut(s) 1963, 2462, 3061
FauI CCCGC 1 cut(s) 614
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 1 cut(s) 2680
Fnu4HI GCNGC 9 cut(s) 162, 696, 1169, 1387, 1571, 1777, 2646, 2794, 3029
FokI GGATG 5 cut(s) 776, 1666, 1784, 2248, 2630
FriOI GRGCYC 1 cut(s) 2439
Fsp4HI GCNGC 9 cut(s) 162, 696, 1169, 1387, 1571, 1777, 2646, 2794, 3029
FspBI CTAG 5 cut(s) 279, 947, 1829, 3020, 3243
GlaI GCGC 1 cut(s) 1260
GluI GCNGC 9 cut(s) 162, 696, 1169, 1387, 1571, 1777, 2646, 2794, 3029
GsaI CCCAGC 1 cut(s) 1415
GsuI CTGGAG 6 cut(s) 369, 1058, 2769, 2823, 2927, 3235
HaeII RGCGCY 1 cut(s) 1262
HaeIII GGCC 8 cut(s) 588, 690, 1238, 1434, 1743, 1869, 2419, 3121
HapII CCGG 6 cut(s) 564, 702, 1392, 2919, 2948, 3110
HgaI GACGC 1 cut(s) 1824
HhaI GCGC 1 cut(s) 1261
Hin6I GCGC 1 cut(s) 1259
HinP1I GCGC 1 cut(s) 1259
HincII GTYRAC 1 cut(s) 1552
HindII GTYRAC 1 cut(s) 1552
HindIII AAGCTT 4 cut(s) 527, 1882, 2288, 2771
HpaII CCGG 6 cut(s) 564, 702, 1392, 2919, 2948, 3110
HphI GGTGA 8 cut(s) 307, 382, 797, 881, 1013, 2266, 2986, 3270
Hpy166II GTNNAC 7 cut(s) 944, 1552, 2012, 2246, 2457, 2681, 3235
Hpy8I GTNNAC 7 cut(s) 944, 1552, 2012, 2246, 2457, 2681, 3235
HpyAV CCTTC 9 cut(s) 96, 151, 175, 313, 1596, 1640, 2917, 2945, 3291
HpyCH4III ACNGT 8 cut(s) 521, 956, 2243, 2736, 2812, 2855, 3232, 3239
HpyCH4IV ACGT 1 cut(s) 2337
HpyF10VI GCNNNNNNNGC 5 cut(s) 535, 1392, 1401, 3037, 3073
HpySE526I ACGT 1 cut(s) 2337
HspAI GCGC 1 cut(s) 1259
Kpn2I TCCGGA 1 cut(s) 563
Ksp22I TGATCA 1 cut(s) 190
LguI GCTCTTC 2 cut(s) 2867, 3093
LmnI GCTCC 5 cut(s) 300, 1215, 2442, 2642, 2842
Lsp1109I GCAGC 9 cut(s) 148, 707, 1180, 1373, 1557, 1788, 2657, 2780, 3040
LweI GCATC 5 cut(s) 81, 1722, 2226, 2608, 3054
MaeI CTAG 5 cut(s) 279, 947, 1829, 3020, 3243
MaeII ACGT 1 cut(s) 2337
MaeIII GTNAC 9 cut(s) 230, 785, 1244, 2063, 2164, 2314, 2522, 2965, 3136
MfeI CAATTG 2 cut(s) 1240, 1331
MflI RGATCY 3 cut(s) 196, 1264, 2943
MhlI GDGCHC 3 cut(s) 946, 2439, 2959
MlsI TGGCCA 2 cut(s) 1238, 2419
MluNI TGGCCA 2 cut(s) 1238, 2419
MlyI GAGTC 4 cut(s) 175, 682, 1449, 2374
MmeI TCCRAC 6 cut(s) 477, 712, 1832, 2408, 2707, 2829
Mox20I TGGCCA 2 cut(s) 1238, 2419
MroI TCCGGA 1 cut(s) 563
MroXI GAANNNNTTC 3 cut(s) 520, 609, 1099
MscI TGGCCA 2 cut(s) 1238, 2419
MseI TTAA 8 cut(s) 894, 987, 1109, 1325, 1406, 1614, 2292, 2304
MslI CAYNNNNRTG 6 cut(s) 205, 996, 2103, 2183, 2402, 2972
Msp20I TGGCCA 2 cut(s) 1238, 2419
MspA1I CMGCKG 1 cut(s) 1570
MspCI CTTAAG 1 cut(s) 1405
MspI CCGG 6 cut(s) 564, 702, 1392, 2919, 2948, 3110
MspR9I CCNGG 8 cut(s) 630, 867, 1198, 2906, 2920, 2948, 3110, 3253
MunI CAATTG 2 cut(s) 1240, 1331
Mva1269I GAATGC 2 cut(s) 933, 2795
MvaI CCWGG 5 cut(s) 630, 867, 1198, 2906, 3253
MvnI CGCG 1 cut(s) 2564
MwoI GCNNNNNNNGC 5 cut(s) 535, 1392, 1401, 3037, 3073
NciI CCSGG 3 cut(s) 2920, 2948, 3110
NcoI CCATGG 1 cut(s) 1213
NlaIV GGNNCC 7 cut(s) 198, 573, 1723, 1795, 1870, 2928, 2956
NmuCI GTSAC 5 cut(s) 785, 1244, 2164, 2314, 2965
NruI TCGCGA 1 cut(s) 2564
OliI CACNNNNGTG 1 cut(s) 2972
PciSI GCTCTTC 2 cut(s) 2867, 3093
PctI GAATGC 2 cut(s) 933, 2795
PdmI GAANNNNTTC 3 cut(s) 520, 609, 1099
PflMI CCANNNNNTGG 1 cut(s) 2846
PfoI TCCNGGA 3 cut(s) 628, 2904, 2946
PkrI GCNGC 9 cut(s) 163, 697, 1170, 1388, 1572, 1778, 2647, 2795, 3030
PleI GAGTC 4 cut(s) 175, 681, 1449, 2374
PpsI GAGTC 4 cut(s) 175, 681, 1449, 2374
PpuMI RGGWCCY 1 cut(s) 2911
PshBI ATTAAT 1 cut(s) 987
Psp124BI GAGCTC 1 cut(s) 2439
Psp1406I AACGTT 1 cut(s) 2337
Psp5II RGGWCCY 1 cut(s) 2911
Psp6I CCWGG 5 cut(s) 628, 865, 1196, 2904, 3251
PspEI GGTNACC 1 cut(s) 2063
PspFI CCCAGC 1 cut(s) 1411
PspGI CCWGG 5 cut(s) 628, 865, 1196, 2904, 3251
PspN4I GGNNCC 7 cut(s) 198, 573, 1723, 1795, 1870, 2928, 2956
PspPI GGNCC 8 cut(s) 1399, 1741, 1868, 1915, 2009, 2729, 2899, 2911
PspPPI RGGWCCY 1 cut(s) 2911
PstI CTGCAG 2 cut(s) 462, 580
PsuI RGATCY 3 cut(s) 196, 1264, 2943
PvuII CAGCTG 1 cut(s) 1570
RruI TCGCGA 1 cut(s) 2564
RsaI GTAC 4 cut(s) 327, 1953, 2627, 2637
RsaNI GTAC 4 cut(s) 326, 1952, 2626, 2636
RseI CAYNNNNRTG 6 cut(s) 205, 996, 2103, 2183, 2402, 2972
SacI GAGCTC 1 cut(s) 2439
SapI GCTCTTC 2 cut(s) 2867, 3093
SaqAI TTAA 8 cut(s) 894, 987, 1109, 1325, 1406, 1614, 2292, 2304
SatI GCNGC 9 cut(s) 162, 696, 1169, 1387, 1571, 1777, 2646, 2794, 3029
Sau96I GGNCC 8 cut(s) 1399, 1741, 1868, 1915, 2009, 2729, 2899, 2911
ScaI AGTACT 1 cut(s) 1953
SchI GAGTC 4 cut(s) 175, 682, 1449, 2374
ScrFI CCNGG 8 cut(s) 630, 867, 1198, 2906, 2920, 2948, 3110, 3253
SduI GDGCHC 3 cut(s) 946, 2439, 2959
SfaNI GCATC 5 cut(s) 81, 1722, 2226, 2608, 3054
SfcI CTRYAG 3 cut(s) 458, 576, 3228
SinI GGWCC 6 cut(s) 1399, 1915, 2009, 2729, 2899, 2911
SmiMI CAYNNNNRTG 6 cut(s) 205, 996, 2103, 2183, 2402, 2972
SmlI CTYRAG 3 cut(s) 1316, 1405, 3149
SmoI CTYRAG 3 cut(s) 1316, 1405, 3149
SsiI CCGC 2 cut(s) 621, 1402
SspI AATATT 1 cut(s) 2199
SspMI CTAG 5 cut(s) 279, 947, 1829, 3020, 3243
SstI GAGCTC 1 cut(s) 2439
StyD4I CCNGG 8 cut(s) 628, 865, 1196, 2904, 2918, 2946, 3108, 3251
StyI CCWWGG 2 cut(s) 1213, 1370
TaaI ACNGT 8 cut(s) 521, 956, 2243, 2736, 2812, 2855, 3232, 3239
TaiI ACGT 1 cut(s) 2340
TaqI TCGA 7 cut(s) 263, 365, 975, 1453, 2553, 2698, 3160
TaqII GACCGA 1 cut(s) 751
TatI WGTACW 3 cut(s) 325, 1951, 2635
Tru1I TTAA 8 cut(s) 894, 987, 1109, 1325, 1406, 1614, 2292, 2304
Tru9I TTAA 8 cut(s) 894, 987, 1109, 1325, 1406, 1614, 2292, 2304
TscAI CASTG 9 cut(s) 352, 733, 1041, 1291, 1795, 2099, 2197, 2248, 2409
TseFI GTSAC 5 cut(s) 785, 1244, 2164, 2314, 2965
TseI GCWGC 9 cut(s) 161, 695, 1168, 1386, 1570, 1776, 2645, 2793, 3028
Tsp45I GTSAC 5 cut(s) 785, 1244, 2164, 2314, 2965
TspGWI ACGGA 1 cut(s) 2996
TspRI CASTG 9 cut(s) 352, 733, 1041, 1291, 1795, 2099, 2197, 2248, 2409
Van91I CCANNNNNTGG 1 cut(s) 2846
Vha464I CTTAAG 1 cut(s) 1405
VneI GTGCAC 1 cut(s) 942
VpaK11BI GGWCC 6 cut(s) 1399, 1915, 2009, 2729, 2899, 2911
VspI ATTAAT 1 cut(s) 987
XagI CCTNNNNNAGG 2 cut(s) 1748, 2906
XbaI TCTAGA 1 cut(s) 3019
XmiI GTMKAC 1 cut(s) 2680
XmnI GAANNNNTTC 3 cut(s) 520, 609, 1099
XspI CTAG 5 cut(s) 279, 947, 1829, 3020, 3243
ZrmI AGTACT 1 cut(s) 1953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.