MD05G1018000.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016498
Physical Location & Seq
Reverse (-)
3119177 .. 3122834
3658 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1018000.v1.1.491

Sequence Viewer

Length: 3267 bp
ATGCCAAGCAATTCAATCACTATCATTCTTTTGCTTCTCAGCATCTTTCTGTTTCCTTCCGTCTCTGCTCTGAACCAAGAGGGTATCTCCCTCCTTTCATGGCTTTCAACCTTCAATTCTTCTTCCTCTGCCGCCTTATTCTCTTCGTGGACTCCCGCTGATCAGGATCCATGCAAATGGGATTATATCAGATGTTCCAGTAACGGGTTTGTTTCCAAAATCACAATCACCTTGATCCATATTCCTACCAGCTTCCCAATCCAGCTCCTCTCCTTCAACTATCTCACCACTCTTGTACTCTCAAATGGGAACCTCACTGGAGAAATCCCACGCGCGATTGGAAACTTGTCTTCGCTTATCACCCTTGATCTCAGCTTCAATGCTCTAACAGGTGATATTCCACAAGAGATAGGAAAGTTATCCAACCTGCGGTTGTTATCACTCAATTCAAATTCATTGCATGGTGCAATTCCCATAGAAATTGGAAACTGTTCAAAGCTTCAACAGCTTGAAGTGTATGACAACCATCTATCCGGAAAGGTTCCTGCAGAAATAGGCCAGTTGTTAGCTCTGGAAGTTTTTCGTGCAGGCGGGAATCCTGGAATTCATGGAGAAATTCCAATGCAAATATCAAAGTGCAAGAATCTAACTATTCTTGGCCTGGCAGCTACCGGAATCACAGGGAAGATTCCAAGCAGTGTTGGAGAACTAAAGAATCTTCAAACTCTTTCGGTCTATACTGCAAACCTTTCAGGTGACATCCCTCCGGAAATTGGTAATTGTTCAGCCTTACGACATTTGTTTCTGTATGACAACCAGCTTTCTGGTCAAATTCCTGGTGAGCTTGGTCTGTTGAAAAGGCTTAAAAGAGTTCTGCTATGGAAGAACAATCTAAGGGGAAGCATTCCGAGCGCACTAGGGAACTGTTTGGATTTGATAGTTATCGATTTCTCAATAAATCTTCTGAGTGGTGAAATTCCTCTCTCTTTTGCAAATTTAGTGGCACTGGAGGAGTTTCTGCTGTCTGAGAATCAGATATCAGGCAACATACCACCCTTATTCGGAAACTTTTCAAACTTAAAGCAACTTGAATTGGACAACAACAGATTTGCGGGCGTGATTCCACCTGTCATTGGGCAGCTGAAAGAACTTACTCTGTTCTATGCCTGGAATAATCAGCTCCATGGAAGCATACCGAATGAGCTCGCCAATTGTGAGAAACTTCAAGCGCTGGATCTTTCACACAATCTCCTCACTGGGTCAGTTCCAAGTTCTCTCTTTGACCTCAAGAACTTAACTCAATTGTTGCTGATATCAAATCAATTTTTAGGAGAAGTTCCAAGGAATATCGGTAGCTGCACCGGCTTGGTCCGCTTAAGGCTGGGATCAAACAACTTCACAGGTCAAATTCCATCAGAACTTGGACTCCTGCATAGATTGAGCTTCCTTGAATTGTCAGAAAATCAATTTACTGGGGAAATTCCTCCTGATATCGGAAACTGCACTCAGTTAGAAATGGTTGACTTGCATGGAAATCAGCTGCAAGGCATGATTCCTTCGTCTTTGCAACTCCTTACTGGTCTTAATGTTTTAGACCTTTCCATGAACCAGATAGAAGGCACGATTCCTGAAGAGTTGGGCAAGTTAACATCCCTAAACAAACTGGTCATCAATGGAAATCGCATCACGGGTTCCATTCCTAAATCGTTGGGCCTATGCAAGGATCTGCAGTTGTTAGATATGAGCAGCAACAATATCACTGGCACCATCCCAGATGAGATCGGACGCTTGCAAGAGCTAGATATTCTCTTGAATCTGAGTTGGAATTCTCTGAGAGGCCCCATTCCAGAAAGCTTCTCAAATCTCTCAAAACTAGCCAATATGGACCTCTCCCATAATTTTTTGACAGGAAGCCTCAGAGTACTGGGTAGTCTTGACAATCTCGTGTCCCTAAATGTCTCACACAATGACTTCTCAGGTCCACTTCCCAATACTAATTTCTTCAAAGACATTCCTTCAACTGCGTTTGCTGGTAACCCGAAGCTGTGTATCAACAGAAATCAGTGCCACTTGAATGACAACCTGCACGACAAGAAATCAATCAGATATCTACTTGTTTGCGTTCTTCTTAGCGTGACTGCAACCATCATGGTTATGCTCGCTGGAATATTTTTATTTGTCAGAATTTGTGGAATCACATTTGGGATGCACAGTGAACAAGAAAATGATCTGGTGTGGGCCTTCACCCCATTTCAAAAGCTTAATTTTTCTGTTAATGATATTGTGACTGGGCTATCAGAATCAAACGTTGTGGGAAAGGGTTGCTCAGGAATGGTTTATCGTGTGGAGACTCCAACGAAGCAGGTCATTGCAGTGAAGAAGCTATGGCCGGTAAATAATGGCGAGCTCCCAGAGAGGGACTTGTTTACGACAGAAGTTCATACTCTTGGATCAATTAGGCATAAAAACATAGTGAGGCTTCTGGGGTGTTGTAACAATGGAAAAACCAGACTTCTTGTGTTTGATTACATCGCGAATGGGAGTTTGGCCAGCTTGCTACACGAGAAGAGGCTGTTCTTGGATTGGGATGCAAGGTACAAGATTGTACTGGGAGCAGCTCAGGGTTTAGCTTATCTCCATCACGATTGTATACCTCCGATAGTTCATCGAGATATCAAGGCTAACAACATCTTGGTTGGACCACAGTATGAATCTTTTATAGCAGATTTTGGCATTGCAAAGCTTTTGAATTCTCCAGAATGCTGCAAAACCTCCAACACAGTTGCAGGTTCCTATGGCTACATTGCTCCAGAATATGGATACAGTTTGAGGATCACAGAGAAGAGCGATGTGTATAGCTATGGAGTGGTGCTCCTAGAGGTCCTGACCGGGAAGGAACCAATGGATAACAAGATCCCGGAAGGTGCCCATATTGTCACTTGGGTGAATACGGAACTTAGAGTAAGGAAAAGAGATTTCACATCAGTTCTAGACCAGCAGCTAATTTTGCTATCAGGGACACAAACCCAAGAGATGCTTCAGGTGCTTGGGGTGGCTCTTCTCTGTGTGAATCCTTGCCCGGAGGAAAGGCCAACTATGAAAGATGTTACAGCAATGCTCAAGGAGATTCAACATGAGAACGAGGATTGTGAAAAACCAGATTTTCTTGGCAAAGGAGTTGCTACAAACCGAAACGCTACAGTTCACAGTTCTAGTTTCTCCAGGTCATCTGAACCTCTAATTGGATCACCTTCTTGTTCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1089

Amino Acids

119.0

Weight (kDa)

5.79

Isoelectric Point (pI)

30.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 66 2.4e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 95 - 153 9.2e-08 Leucine rich repeat
LRR_14 PF23598 98 - 195 2.7e-09 Leucine-rich repeat region
LRR_14 PF23598 204 - 310 1.4e-08 Leucine-rich repeat region
LRR_14 PF23598 473 - 556 1.9e-07 Leucine-rich repeat region
LRR_8 PF13855 503 - 562 9.6e-08 Leucine rich repeat
Pkinase PF00069 768 - 1037 1.5e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 768 - 1038 1.4e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016498)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g14410
malus_domestica MD05G1018000.v1.1
prunus_persica Prupe.8G022000_v2.0.a1
pyrus_communis pycom05g01070
rosa_chinensis RchiOBHm_Chr6g0276741
rosa_laevigata RLG00000013367
rosa_multiflora Rmu_sc0004481.1_g000026
rosa_roxburghii Rroxscaffold_7G00192010
rosa_rugosa Rorug06G0103400
rosa_samantha Rh6AG213800 Rh6BG218600 Rh6CG221600 Rh6DG211300
rosa_wichuraiana Rw6G018690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 435, 2091, 2353, 2777
AccB1I GGYRCC 2 cut(s) 1763, 2924
AccB7I CCANNNNNTGG 1 cut(s) 2816
AccI GTMKAC 1 cut(s) 2650
AccII CGCG 3 cut(s) 333, 335, 2534
AccIII TCCGGA 2 cut(s) 533, 766
AciI CCGC 6 cut(s) 132, 156, 430, 591, 1112, 1372
AclI AACGTT 1 cut(s) 2307
AcoI YGGCCR 2 cut(s) 2387, 2547
AcuI CTGAAG 2 cut(s) 1650, 3023
AfaI GTAC 4 cut(s) 297, 1923, 2597, 2607
AfeI AGCGCT 1 cut(s) 1230
AflII CTTAAG 1 cut(s) 1375
AjnI CCWGG 5 cut(s) 598, 660, 835, 1166, 3221
AjuI GAANNNNNNNTTGG 8 cut(s) 1982, 2014, 2528, 2560, 3021, 3053, 3225, 3257
AleI CACNNNNGTG 1 cut(s) 2942
AloI GAACNNNNNNTCC 2 cut(s) 1410, 1442
Alw21I GWGCWC 3 cut(s) 1206, 2409, 2874
Alw26I GTCTC 3 cut(s) 67, 1963, 2342
Aor13HI TCCGGA 2 cut(s) 533, 766
Aor51HI AGCGCT 1 cut(s) 1230
AoxI GGCC 8 cut(s) 556, 658, 1711, 1837, 2238, 2387, 2547, 3089
ApeKI GCWGC 8 cut(s) 665, 1138, 1356, 1540, 1746, 2615, 2763, 2998
Asp700I GAANNNNTTC 3 cut(s) 490, 579, 1069
AspLEI GCGC 3 cut(s) 335, 914, 1231
AspS9I GGNCC 8 cut(s) 1369, 1711, 1838, 1885, 1979, 2238, 2699, 2881
AsuC2I CCSGG 3 cut(s) 2890, 2918, 3080
AvaII GGWCC 5 cut(s) 1369, 1885, 1979, 2699, 2881
BaeGI GKGCMC 1 cut(s) 2929
BalI TGGCCA 1 cut(s) 2549
BamHI GGATCC 1 cut(s) 166
BanI GGYRCC 2 cut(s) 1763, 2924
BanII GRGCYC 2 cut(s) 1206, 2409
BauI CACGAG 2 cut(s) 1943, 2561
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 3 cut(s) 1206, 2409, 2874
BbvI GCAGC 8 cut(s) 677, 1150, 1343, 1527, 1758, 2627, 2750, 3010
BccI CCATC 5 cut(s) 534, 1420, 1775, 2153, 2646
BciT130I CCWGG 5 cut(s) 600, 662, 837, 1168, 3223
BciVI GTATCC 1 cut(s) 2813
BclI TGATCA 1 cut(s) 160
BcnI CCSGG 3 cut(s) 2890, 2918, 3080
BcoDI GTCTC 3 cut(s) 67, 1963, 2342
BfaI CTAG 6 cut(s) 917, 1799, 1874, 2876, 2990, 3213
BfmI CTRYAG 3 cut(s) 546, 1727, 3198
BfoI RGCGCY 1 cut(s) 1232
BfrI CTTAAG 1 cut(s) 1375
BfuAI ACCTGC 4 cut(s) 435, 2091, 2353, 2777
BfuI GTATCC 1 cut(s) 2813
BisI GCNGC 9 cut(s) 132, 666, 1139, 1357, 1541, 1747, 2616, 2764, 2999
BlsI GCNGC 9 cut(s) 133, 667, 1140, 1358, 1542, 1748, 2617, 2765, 3000
BmcAI AGTACT 1 cut(s) 1923
Bme1390I CCNGG 8 cut(s) 600, 662, 837, 1168, 2890, 2918, 3080, 3223
Bme18I GGWCC 5 cut(s) 1369, 1885, 1979, 2699, 2881
BmgT120I GGNCC 8 cut(s) 1369, 1711, 1838, 1885, 1979, 2238, 2699, 2881
BmiI GGNNCC 9 cut(s) 168, 311, 543, 1693, 1765, 1840, 2791, 2898, 2926
BmrFI CCNGG 8 cut(s) 600, 662, 837, 1168, 2890, 2918, 3080, 3223
BmrI ACTGGG 5 cut(s) 1266, 1482, 1934, 2298, 2618
BmsI GCATC 5 cut(s) 51, 1692, 2196, 2578, 3024
BmuI ACTGGG 5 cut(s) 1266, 1482, 1934, 2298, 2618
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 4 cut(s) 71, 103, 2856, 2888
BpmI CTGGAG 5 cut(s) 339, 1028, 2739, 2793, 3205
Bpu10I CCTNAGC 2 cut(s) 2326, 2619
BpuEI CTTGAG 2 cut(s) 1271, 3104
BpuMI CCSGG 3 cut(s) 2890, 2918, 3080
Bsa29I ATCGAT 1 cut(s) 945
BsaBI GATNNNNATC 2 cut(s) 165, 941
BsaJI CCNNGG 2 cut(s) 1183, 1340
BsaWI WCCGGW 3 cut(s) 533, 671, 766
BsaXI ACNNNNNCTCC 4 cut(s) 1233, 1263, 1410, 1440
Bse118I RCCGGY 2 cut(s) 1361, 2389
Bse3DI GCAATG 5 cut(s) 455, 2367, 2733, 2802, 3120
Bse8I GATNNNNATC 2 cut(s) 165, 941
BseAI TCCGGA 2 cut(s) 533, 766
BseBI CCWGG 5 cut(s) 600, 662, 837, 1168, 3223
BseCI ATCGAT 1 cut(s) 945
BseDI CCNNGG 2 cut(s) 1183, 1340
BseGI GGATG 5 cut(s) 759, 1649, 1767, 2211, 2593
BseJI GATNNNNATC 2 cut(s) 165, 941
BseMI GCAATG 5 cut(s) 455, 2367, 2733, 2802, 3120
BseRI GAGGAG 3 cut(s) 257, 1025, 1241
BseSI GKGCMC 1 cut(s) 2929
BseXI GCAGC 8 cut(s) 677, 1150, 1343, 1527, 1758, 2627, 2750, 3010
BseYI CCCAGC 1 cut(s) 1381
BsgI GTGCAG 4 cut(s) 606, 1342, 1486, 2069
Bsh1236I CGCG 3 cut(s) 333, 335, 2534
BshFI GGCC 8 cut(s) 558, 660, 1713, 1839, 2240, 2389, 2549, 3091
BshNI GGYRCC 2 cut(s) 1763, 2924
BshVI ATCGAT 1 cut(s) 945
BsiHKAI GWGCWC 3 cut(s) 1206, 2409, 2874
BsiSI CCGG 8 cut(s) 534, 672, 767, 1362, 2390, 2889, 2918, 3080
BslFI GGGAC 3 cut(s) 1933, 2432, 3031
BsmAI GTCTC 3 cut(s) 67, 1963, 2342
BsmBI CGTCTC 1 cut(s) 67
BsmFI GGGAC 3 cut(s) 1933, 2432, 3031
BsmI GAATGC 2 cut(s) 903, 2765
BsnI GGCC 8 cut(s) 558, 660, 1713, 1839, 2240, 2389, 2549, 3091
Bsp1286I GDGCHC 4 cut(s) 1206, 2409, 2874, 2929
Bsp13I TCCGGA 2 cut(s) 533, 766
Bsp19I CCATGG 1 cut(s) 1183
Bsp68I TCGCGA 1 cut(s) 2534
BspACI CCGC 6 cut(s) 132, 156, 430, 591, 1112, 1372
BspANI GGCC 8 cut(s) 558, 660, 1713, 1839, 2240, 2389, 2549, 3091
BspDI ATCGAT 1 cut(s) 945
BspEI TCCGGA 2 cut(s) 533, 766
BspFNI CGCG 3 cut(s) 333, 335, 2534
BspLI GGNNCC 9 cut(s) 168, 311, 543, 1693, 1765, 1840, 2791, 2898, 2926
BspMAI CTGCAG 2 cut(s) 550, 1731
BspMI ACCTGC 4 cut(s) 435, 2091, 2353, 2777
BspQI GCTCTTC 2 cut(s) 2837, 3063
BspT107I GGYRCC 2 cut(s) 1763, 2924
BspTI CTTAAG 1 cut(s) 1375
BsrDI GCAATG 5 cut(s) 455, 2367, 2733, 2802, 3120
BsrFI RCCGGY 2 cut(s) 1361, 2389
BssAI RCCGGY 2 cut(s) 1361, 2389
BssECI CCNNGG 2 cut(s) 1183, 1340
BssNAI GTATAC 1 cut(s) 2651
BssSI CACGAG 2 cut(s) 1943, 2561
BssT1I CCWWGG 2 cut(s) 1183, 1340
Bst1107I GTATAC 1 cut(s) 2651
Bst2BI CACGAG 2 cut(s) 1943, 2561
Bst2UI CCWGG 5 cut(s) 600, 662, 837, 1168, 3223
Bst4CI ACNGT 8 cut(s) 491, 926, 2213, 2706, 2782, 2825, 3202, 3209
Bst6I CTCTTC 5 cut(s) 148, 1626, 2561, 2837, 3063
BstAFI CTTAAG 1 cut(s) 1375
BstC8I GCNNGC 8 cut(s) 589, 1114, 1206, 1790, 2160, 2405, 2551, 2555
BstDSI CCRYGG 1 cut(s) 1183
BstEII GGTNACC 1 cut(s) 2033
BstENI CCTNNNNNAGG 1 cut(s) 1718
BstF5I GGATG 5 cut(s) 759, 1649, 1767, 2211, 2593
BstFNI CGCG 3 cut(s) 333, 335, 2534
BstH2I RGCGCY 1 cut(s) 1232
BstHHI GCGC 3 cut(s) 335, 914, 1231
BstMAI GTCTC 3 cut(s) 67, 1963, 2342
BstMWI GCNNNNNNNGC 6 cut(s) 505, 909, 1362, 1371, 3007, 3043
BstNI CCWGG 5 cut(s) 600, 662, 837, 1168, 3223
BstPI GGTNACC 1 cut(s) 2033
BstSCI CCNGG 8 cut(s) 598, 660, 835, 1166, 2888, 2916, 3078, 3221
BstSFI CTRYAG 3 cut(s) 546, 1727, 3198
BstSLI GKGCMC 1 cut(s) 2929
BstUI CGCG 3 cut(s) 333, 335, 2534
BstV1I GCAGC 8 cut(s) 677, 1150, 1343, 1527, 1758, 2627, 2750, 3010
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 4 cut(s) 166, 1234, 1723, 2913
BstXI CCANNNNNNTGG 2 cut(s) 177, 824
BstYI RGATCY 4 cut(s) 166, 1234, 1723, 2913
BstZ17I GTATAC 1 cut(s) 2651
Bsu15I ATCGAT 1 cut(s) 945
BsuI GTATCC 1 cut(s) 2813
BsuRI GGCC 8 cut(s) 558, 660, 1713, 1839, 2240, 2389, 2549, 3091
BsuTUI ATCGAT 1 cut(s) 945
BtgI CCRYGG 1 cut(s) 1183
BtgZI GCGATG 2 cut(s) 2515, 2862
BtsCI GGATG 5 cut(s) 759, 1649, 1767, 2211, 2593
BtsI GCAGTG 2 cut(s) 703, 2379
BtsIMutI CAGTG 8 cut(s) 315, 703, 1004, 1254, 1758, 2069, 2218, 2379
BtuMI TCGCGA 1 cut(s) 2534
BveI ACCTGC 4 cut(s) 435, 2091, 2353, 2777
Cac8I GCNNGC 8 cut(s) 589, 1114, 1206, 1790, 2160, 2405, 2551, 2555
CfoI GCGC 3 cut(s) 335, 914, 1231
Cfr10I RCCGGY 2 cut(s) 1361, 2389
Cfr13I GGNCC 8 cut(s) 1369, 1711, 1838, 1885, 1979, 2238, 2699, 2881
ClaI ATCGAT 1 cut(s) 945
CseI GACGC 1 cut(s) 1794
Csp6I GTAC 4 cut(s) 296, 1922, 2596, 2606
CspCI CAANNNNNGTGG 4 cut(s) 981, 1016, 2292, 2327
CviQI GTAC 4 cut(s) 296, 1922, 2596, 2606
EaeI YGGCCR 2 cut(s) 2387, 2547
Eam1104I CTCTTC 5 cut(s) 148, 1626, 2561, 2837, 3063
EarI CTCTTC 5 cut(s) 148, 1626, 2561, 2837, 3063
Ecl136II GAGCTC 2 cut(s) 1204, 2407
Eco130I CCWWGG 2 cut(s) 1183, 1340
Eco24I GRGCYC 2 cut(s) 1206, 2409
Eco32I GATATC 5 cut(s) 1038, 1314, 1492, 2108, 2674
Eco47I GGWCC 5 cut(s) 1369, 1885, 1979, 2699, 2881
Eco47III AGCGCT 1 cut(s) 1230
Eco53kI GAGCTC 2 cut(s) 1204, 2407
Eco57I CTGAAG 2 cut(s) 1650, 3023
Eco91I GGTNACC 1 cut(s) 2033
EcoICRI GAGCTC 2 cut(s) 1204, 2407
EcoNI CCTNNNNNAGG 1 cut(s) 1718
EcoO109I RGGNCCY 2 cut(s) 1838, 2881
EcoO65I GGTNACC 1 cut(s) 2033
EcoRI GAATTC 3 cut(s) 603, 1825, 2749
EcoRII CCWGG 5 cut(s) 598, 660, 835, 1166, 3221
EcoRV GATATC 5 cut(s) 1038, 1314, 1492, 2108, 2674
EcoT14I CCWWGG 2 cut(s) 1183, 1340
EcoT38I GRGCYC 2 cut(s) 1206, 2409
ErhI CCWWGG 2 cut(s) 1183, 1340
Esp3I CGTCTC 1 cut(s) 67
FalI AAGNNNNNCTT 2 cut(s) 3021, 3053
FaqI GGGAC 3 cut(s) 1933, 2432, 3031
FauI CCCGC 3 cut(s) 163, 584, 1105
FbaI TGATCA 1 cut(s) 160
FblI GTMKAC 1 cut(s) 2650
Fnu4HI GCNGC 9 cut(s) 132, 666, 1139, 1357, 1541, 1747, 2616, 2764, 2999
FokI GGATG 5 cut(s) 746, 1636, 1754, 2218, 2600
FriOI GRGCYC 2 cut(s) 1206, 2409
Fsp4HI GCNGC 9 cut(s) 132, 666, 1139, 1357, 1541, 1747, 2616, 2764, 2999
FspBI CTAG 6 cut(s) 917, 1799, 1874, 2876, 2990, 3213
GlaI GCGC 3 cut(s) 334, 913, 1230
GluI GCNGC 9 cut(s) 132, 666, 1139, 1357, 1541, 1747, 2616, 2764, 2999
GsaI CCCAGC 1 cut(s) 1385
GsuI CTGGAG 5 cut(s) 339, 1028, 2739, 2793, 3205
HaeII RGCGCY 1 cut(s) 1232
HaeIII GGCC 8 cut(s) 558, 660, 1713, 1839, 2240, 2389, 2549, 3091
HapII CCGG 8 cut(s) 534, 672, 767, 1362, 2390, 2889, 2918, 3080
HgaI GACGC 1 cut(s) 1794
HhaI GCGC 3 cut(s) 335, 914, 1231
Hin6I GCGC 3 cut(s) 333, 912, 1229
HinP1I GCGC 3 cut(s) 333, 912, 1229
HincII GTYRAC 2 cut(s) 1522, 1647
HindII GTYRAC 2 cut(s) 1522, 1647
HindIII AAGCTT 4 cut(s) 497, 1852, 2258, 2741
HpaI GTTAAC 1 cut(s) 1647
HpaII CCGG 8 cut(s) 534, 672, 767, 1362, 2390, 2889, 2918, 3080
Hpy166II GTNNAC 8 cut(s) 150, 1522, 1647, 1982, 2216, 2427, 2651, 3205
Hpy8I GTNNAC 8 cut(s) 150, 1522, 1647, 1982, 2216, 2427, 2651, 3205
HpyCH4III ACNGT 8 cut(s) 491, 926, 2213, 2706, 2782, 2825, 3202, 3209
HpyCH4IV ACGT 1 cut(s) 2307
HpyF10VI GCNNNNNNNGC 6 cut(s) 505, 909, 1362, 1371, 3007, 3043
HpySE526I ACGT 1 cut(s) 2307
HspAI GCGC 3 cut(s) 333, 912, 1229
Kpn2I TCCGGA 2 cut(s) 533, 766
Ksp22I TGATCA 1 cut(s) 160
KspAI GTTAAC 1 cut(s) 1647
LguI GCTCTTC 2 cut(s) 2837, 3063
LmnI GCTCC 6 cut(s) 270, 1185, 2412, 2612, 2812, 2877
Lsp1109I GCAGC 8 cut(s) 677, 1150, 1343, 1527, 1758, 2627, 2750, 3010
LweI GCATC 5 cut(s) 51, 1692, 2196, 2578, 3024
MaeI CTAG 6 cut(s) 917, 1799, 1874, 2876, 2990, 3213
MaeII ACGT 1 cut(s) 2307
MaeIII GTNAC 8 cut(s) 200, 755, 2033, 2134, 2284, 2492, 2935, 3106
MfeI CAATTG 2 cut(s) 1210, 1301
MflI RGATCY 4 cut(s) 166, 1234, 1723, 2913
MhlI GDGCHC 4 cut(s) 1206, 2409, 2874, 2929
MlsI TGGCCA 1 cut(s) 2549
MluNI TGGCCA 1 cut(s) 2549
MlyI GAGTC 3 cut(s) 145, 1419, 2344
MmeI TCCRAC 6 cut(s) 447, 682, 1802, 2378, 2677, 2799
Mox20I TGGCCA 1 cut(s) 2549
MroI TCCGGA 2 cut(s) 533, 766
MroXI GAANNNNTTC 3 cut(s) 490, 579, 1069
MscI TGGCCA 1 cut(s) 2549
MseI TTAA 8 cut(s) 864, 1079, 1295, 1376, 1584, 1646, 2262, 2274
MslI CAYNNNNRTG 5 cut(s) 175, 2073, 2153, 2372, 2942
Msp20I TGGCCA 1 cut(s) 2549
MspA1I CMGCKG 3 cut(s) 158, 1141, 1540
MspCI CTTAAG 1 cut(s) 1375
MspI CCGG 8 cut(s) 534, 672, 767, 1362, 2390, 2889, 2918, 3080
MspR9I CCNGG 8 cut(s) 600, 662, 837, 1168, 2890, 2918, 3080, 3223
MunI CAATTG 2 cut(s) 1210, 1301
Mva1269I GAATGC 2 cut(s) 903, 2765
MvaI CCWGG 5 cut(s) 600, 662, 837, 1168, 3223
MvnI CGCG 3 cut(s) 333, 335, 2534
MwoI GCNNNNNNNGC 6 cut(s) 505, 909, 1362, 1371, 3007, 3043
NciI CCSGG 3 cut(s) 2890, 2918, 3080
NcoI CCATGG 1 cut(s) 1183
NlaIV GGNNCC 9 cut(s) 168, 311, 543, 1693, 1765, 1840, 2791, 2898, 2926
NmuCI GTSAC 4 cut(s) 755, 2134, 2284, 2935
NruI TCGCGA 1 cut(s) 2534
OliI CACNNNNGTG 1 cut(s) 2942
PciSI GCTCTTC 2 cut(s) 2837, 3063
PctI GAATGC 2 cut(s) 903, 2765
PdmI GAANNNNTTC 3 cut(s) 490, 579, 1069
PflMI CCANNNNNTGG 1 cut(s) 2816
PfoI TCCNGGA 2 cut(s) 598, 2916
PkrI GCNGC 9 cut(s) 133, 667, 1140, 1358, 1542, 1748, 2617, 2765, 3000
PleI GAGTC 3 cut(s) 145, 1419, 2344
PpsI GAGTC 3 cut(s) 145, 1419, 2344
PpuMI RGGWCCY 1 cut(s) 2881
Psp124BI GAGCTC 2 cut(s) 1206, 2409
Psp1406I AACGTT 1 cut(s) 2307
Psp5II RGGWCCY 1 cut(s) 2881
Psp6I CCWGG 5 cut(s) 598, 660, 835, 1166, 3221
PspEI GGTNACC 1 cut(s) 2033
PspFI CCCAGC 1 cut(s) 1381
PspGI CCWGG 5 cut(s) 598, 660, 835, 1166, 3221
PspN4I GGNNCC 9 cut(s) 168, 311, 543, 1693, 1765, 1840, 2791, 2898, 2926
PspPI GGNCC 8 cut(s) 1369, 1711, 1838, 1885, 1979, 2238, 2699, 2881
PspPPI RGGWCCY 1 cut(s) 2881
PstI CTGCAG 2 cut(s) 550, 1731
PsuI RGATCY 4 cut(s) 166, 1234, 1723, 2913
PvuII CAGCTG 2 cut(s) 1141, 1540
RruI TCGCGA 1 cut(s) 2534
RsaI GTAC 4 cut(s) 297, 1923, 2597, 2607
RsaNI GTAC 4 cut(s) 296, 1922, 2596, 2606
RseI CAYNNNNRTG 5 cut(s) 175, 2073, 2153, 2372, 2942
SacI GAGCTC 2 cut(s) 1206, 2409
SapI GCTCTTC 2 cut(s) 2837, 3063
SaqAI TTAA 8 cut(s) 864, 1079, 1295, 1376, 1584, 1646, 2262, 2274
SatI GCNGC 9 cut(s) 132, 666, 1139, 1357, 1541, 1747, 2616, 2764, 2999
Sau96I GGNCC 8 cut(s) 1369, 1711, 1838, 1885, 1979, 2238, 2699, 2881
ScaI AGTACT 1 cut(s) 1923
SchI GAGTC 3 cut(s) 145, 1419, 2344
ScrFI CCNGG 8 cut(s) 600, 662, 837, 1168, 2890, 2918, 3080, 3223
SduI GDGCHC 4 cut(s) 1206, 2409, 2874, 2929
SfaNI GCATC 5 cut(s) 51, 1692, 2196, 2578, 3024
SfcI CTRYAG 3 cut(s) 546, 1727, 3198
SinI GGWCC 5 cut(s) 1369, 1885, 1979, 2699, 2881
SmiMI CAYNNNNRTG 5 cut(s) 175, 2073, 2153, 2372, 2942
SmlI CTYRAG 3 cut(s) 1286, 1375, 3119
SmoI CTYRAG 3 cut(s) 1286, 1375, 3119
SsiI CCGC 6 cut(s) 132, 156, 430, 591, 1112, 1372
SspI AATATT 1 cut(s) 2169
SspMI CTAG 6 cut(s) 917, 1799, 1874, 2876, 2990, 3213
SstI GAGCTC 2 cut(s) 1206, 2409
StyD4I CCNGG 8 cut(s) 598, 660, 835, 1166, 2888, 2916, 3078, 3221
StyI CCWWGG 2 cut(s) 1183, 1340
TaaI ACNGT 8 cut(s) 491, 926, 2213, 2706, 2782, 2825, 3202, 3209
TaiI ACGT 1 cut(s) 2310
TaqI TCGA 2 cut(s) 945, 2668
TaqII GACCGA 1 cut(s) 721
TatI WGTACW 3 cut(s) 295, 1921, 2605
TauI GCSGC 1 cut(s) 134
Tru1I TTAA 8 cut(s) 864, 1079, 1295, 1376, 1584, 1646, 2262, 2274
Tru9I TTAA 8 cut(s) 864, 1079, 1295, 1376, 1584, 1646, 2262, 2274
TscAI CASTG 8 cut(s) 322, 703, 1011, 1261, 1765, 2069, 2218, 2379
TseFI GTSAC 4 cut(s) 755, 2134, 2284, 2935
TseI GCWGC 8 cut(s) 665, 1138, 1356, 1540, 1746, 2615, 2763, 2998
Tsp45I GTSAC 4 cut(s) 755, 2134, 2284, 2935
TspDTI ATGAA 8 cut(s) 87, 444, 596, 1619, 2429, 2654, 2724, 3113
TspGWI ACGGA 2 cut(s) 49, 2966
TspRI CASTG 8 cut(s) 322, 703, 1011, 1261, 1765, 2069, 2218, 2379
Van91I CCANNNNNTGG 1 cut(s) 2816
Vha464I CTTAAG 1 cut(s) 1375
VpaK11BI GGWCC 5 cut(s) 1369, 1885, 1979, 2699, 2881
XagI CCTNNNNNAGG 1 cut(s) 1718
XbaI TCTAGA 1 cut(s) 2989
XmiI GTMKAC 1 cut(s) 2650
XmnI GAANNNNTTC 3 cut(s) 490, 579, 1069
XspI CTAG 6 cut(s) 917, 1799, 1874, 2876, 2990, 3213
ZrmI AGTACT 1 cut(s) 1923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.