RLG00000013367
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016498
Physical Location & Seq
Reverse (-)
30366037 .. 30369491
3455 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013367

Sequence Viewer

Length: 3273 bp
ATGTCAACTAATGCAATCACTATTATTCTCTTGTTTCTAAACATCTCTCTATTTCCTGCCATCTCTGCTCTGAACCAAGAGGGTCTCTCCCTGCTTTCATGGCTTTCAACCTTCAACTCTTCTTCCTCAGCTGCCCTTTTTTCTTCCTGGAATCCAACTGCCTATGATCCATGCAAATGGGATTATATCAGATGTTCTGAAGCGGGATTTGTTTCAGAAATCACAATAACCTCAATCAATATACAAACCAGGTTCCCCATCCAGCTACTCACCTTTAATCATCTCACCACCCTTGTAATCTCAAACGCCAACCTCAGTGGAGAAATTCCACAGGCAATTGGAAACTTGTCATCACTCATCACCCTGGATCTCAGCTTCAATGCTCTAAGAGGTTACGTCCCACGGGAGATGGGAAAATTATCACAACTGCAGTTGTTATCACTGAATTCAAATTCCTTGGATGGTGCAATGCCAAAAGAAATCGGAAACTGTTCAGAGCTTCAACAACTTGAACTGTACGATAACCAGCTATCTGGAAAGATTCCTGCTGAAATAGGGCAGTTGTGGGCTCTGGAAATCTTCCGTGCAGGCGGGAATCCTGACATTCAAGGTGAAATTCCAATGCAGATATCAAACTGCAAGGGACTAACTTTCCTTGGCCTTGCAGCTACAGGAATCAGGGGGAAAATTCCAAGCAGCGTAGGAGAACTTAAGAATCTCCAAACTCTTTCAATTTATACTGCAAATCTCTCAGGTGAGATTCCACCAGAAATTGGTAACTGTTCAGCCTTACAAAATTTGTTTCTGTATGATAACCAGCTTGCTGGTCAAATTCCTGGTGAACTTGGTCTGTTGAAAAAACTCAGAAGAGTGCTACTGTGGAAGAACAATCTCAATGGAAGCATCCCAGACACACTAGGGAACTGTTCAAAATTGAAAGTAGTCGATTTCTCGTTGAATCTTCTGAGTGGTGAGGTTCCTTTGTCTCTTGCAAATTTGGTGGCATTGGAGGAGCTTCTTCTATCTGAGAATCAGATATCAGGCAGCATACCGCCTTTACTTGGAAATTTTTCCAGTTTAAAGCAACTGGAATTGGATAACAACAGATTTTCGGGTACAATTCCACCTGTCATTGGGCAACTAAAAGAACTTACTCTTTTCTTTGCCTGGCAGAATCAGCTCCAAGGAAGCATACCAACTGAGCTGGCCAACTGTCAGAAACTTCAAGCATTGGATCTCTCACATAATCTCCTCAGTGGATCAGTGCCGAGTTCTCTGTTTGACCTCAAGAACTTAACACAACTGTTGCTGATTTCAAATGAAATTTCAGGACAACTTCCAACCAATATTGGTAACTGTACCAATTTGATCCGCTTACGCCTCGGATCAAACAACTTCACAGGTCAGATACCATCCGAAATTCGGCTCCTGCAGAGATTGAGCTTCCTAGAATTGTCAGAAAATCATTTTACTGGGAAAATTCCTCCTGAAATAGGAGACTGCTCCCACCTTGAAATGGTTGATTTGCATGGAAATGAGCTCCGAGGCAGGATTCCTTCGTCTTTCCAATTCCTTACTGGTCTTAATGTTTTAGACCTGTCCATGAACCAAATAGAAGGCACAGTTCCTGAACATTTGGGGAAGCTGAAATCCTTGAACAAACTGGTGGTCAATGGAAACTACATTACTGGTTCCATTCCCAAGTCATTGAGCCTCTGTAGGGATTTGCAGTTGTTGGAACTGAGCAGTAACAGACTTACAGGCTCTATCCCAGATGAGATTGGACACTTGCAAGGGCTCGATATTCTCTTGAATCTGAGCTGGAATTCTCTGACAGGCCCCATTCCAGAAAGCTTCTCAAATCTCTCAAAACTTGCCACTATGGATCTCTCCCACAATATGCTGACAGGAAGCCTGAAAGTACTGGGGGTTCTGGACAACCTCGTCTCCCTGAATGTCTCATACAATGACTTTTCAGGTCTACTTCCCAGTACTAATTTTTTCAAAGATCTTCCACCAACTGCATTTGCTGGTAATCACGAGTTATGCATCAACCGAAACAAGTGCCAACCAAATAGAAACCTGCATGACAAAAAATCCATCAGATATCTAATTATTTGTATCCTTCTCAGTATAACTGCAACTATATTTCTTATGCTTGCTGGAATAGCTTTGTTTGTCAGAGTCTGGGGAATCACATTTGGGATTACGAACAGTAAAGAAGAAAATGATCTGGTGTGGGATTTCACCCCATTTCAAAAGCTTGATTTTGCAGTTAATGACATTGTGACTAAGCTATCAGACTCGAACATTGTTGGGAAGGGAGGCTCAGGAATGGTTTATCGTGTAGAGACTCCAACGAGACAGGTCATTGCAGTGAAGAAGTTATGGCCGATAAACAATGGGGAGCTTCCAGAAAGGGACCTTTTCTCTACAGAAGTTCATACTCTTGGATCGATAAGGCATAAGAATATAGTGAGGCTTTTGGGCTGTTGTAAAAATGGGAAAACCAGATTGCTTTTGTTTGATTACATCAGTAATGGGAGCTTAGCTGGACTGCTCCATGAGAAGAGGCTGTTCTTGGACTGGGATACAAGGTACAAGATTGTACTGGGAGCAGCTCATGGCCTAGCTTATCTTCATCATGATTGTGTTCCTCCAATCATTCATCGAGATATTAAGGCAAACAACATCTTGGTAGGGCCACAGTATGAATCTCTTCTTGCAGATTTTGGCCTTGCAAAGCTGTTGAGTTCTCCAGAATGCTGCAGAGTTTCCAACACAGTTGCAGGTTCTTATGGGTACATTGCTCCAGAATATGGATACAGTTTGAGGATCACAGAAAAGAGTGACGTGTATAGCTATGGAGTGGTGCTCCTAGAGGTCCTAACAGGGAAGGAACCAACAGATAACAAGATTCCAGAGGGTGATCATATTGTCACTTGGGTTAATAAGGAACTTAGAGAGAGAAAAAGAGAATTCACATCAATTGTTGATCATCAGCTAATACTGAGATCAGGGACACAAATTCAAGAAATGCTTCAAGTTCTTGGGGTAGCTCTTCTCTGTGTTAATCCCTGCCCGGAGGAAAGGCCGACAATGAAAGATGTAACAGCAATGCTCAATGAGATCAAGCATGAAAATGAAGATTGTGAAAAACCAAATTTTCTCGGCCAAGGAGCTGTGACAAACAGGAAAACTGCAGTTTACAGTTCTAGTTTCTCCAGGTCATCTGAACCTCTAATTGGTTCACCTTCTTGTTTTCCCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1091

Amino Acids

119.94

Weight (kDa)

5.99

Isoelectric Point (pI)

29.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 66 4.1e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 92 - 194 1.5e-10 Leucine-rich repeat region
LRR_8 PF13855 94 - 153 4.2e-08 Leucine rich repeat
LRR_14 PF23598 205 - 316 3.9e-09 Leucine-rich repeat region
LRR_8 PF13855 287 - 346 8.2e-06 Leucine rich repeat
LRR_14 PF23598 473 - 556 1.8e-07 Leucine-rich repeat region
LRR_8 PF13855 502 - 562 3.6e-08 Leucine rich repeat
Pkinase PF00069 769 - 1038 2.8e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 770 - 1040 4.4e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016498)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g14410
malus_domestica MD05G1018000.v1.1
prunus_persica Prupe.8G022000_v2.0.a1
pyrus_communis pycom05g01070
rosa_chinensis RchiOBHm_Chr6g0276741
rosa_laevigata RLG00000013367
rosa_multiflora Rmu_sc0004481.1_g000026
rosa_roxburghii Rroxscaffold_7G00192010
rosa_rugosa Rorug06G0103400
rosa_samantha Rh6AG213800 Rh6BG218600 Rh6CG221600 Rh6DG211300
rosa_wichuraiana Rw6G018690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1943
Acc36I ACCTGC 2 cut(s) 2091, 2780
AccB7I CCANNNNNTGG 2 cut(s) 773, 2819
AccI GTMKAC 1 cut(s) 1981
AciI CCGC 4 cut(s) 203, 591, 1052, 1372
AclWI GGATC 9 cut(s) 161, 375, 1242, 1267, 1363, 1393, 1893, 2461, 2843
AcoI YGGCCR 3 cut(s) 1206, 2390, 3172
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 8 cut(s) 518, 1117, 1360, 1923, 1993, 2600, 2610, 2804
AfiI CCNNNNNNNGG 9 cut(s) 773, 1061, 1133, 1422, 1493, 1516, 1720, 2819, 3245
AflII CTTAAG 1 cut(s) 710
AflIII ACRYGT 1 cut(s) 2853
AjiI CACGTC 1 cut(s) 2854
AjnI CCWGG 6 cut(s) 146, 248, 363, 835, 1166, 3224
AjuI GAANNNNNNNTTGG 2 cut(s) 3228, 3260
Alw21I GWGCWC 2 cut(s) 1542, 2877
Alw26I GTCTC 7 cut(s) 89, 990, 1491, 1951, 1963, 2345, 2356
AlwI GGATC 9 cut(s) 161, 375, 1242, 1267, 1363, 1393, 1893, 2461, 2843
AlwNI CAGNNNCTG 2 cut(s) 1628, 2187
AoxI GGCC 9 cut(s) 658, 1206, 1837, 2390, 2626, 2702, 2734, 3092, 3172
ApeKI GCWGC 6 cut(s) 131, 665, 696, 1044, 2618, 2766
ArsI GACNNNNNNTTYG 2 cut(s) 1653, 1685
Asp700I GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
AspS9I GGNCC 4 cut(s) 1838, 2422, 2702, 2884
AsuC2I CCSGG 1 cut(s) 3083
AvaII GGWCC 2 cut(s) 2422, 2884
BalI TGGCCA 1 cut(s) 1208
BanII GRGCYC 3 cut(s) 571, 1542, 1800
BauI CACGAG 1 cut(s) 2039
Bbv12I GWGCWC 2 cut(s) 1542, 2877
BbvCI CCTCAGC 1 cut(s) 127
BbvI GCAGC 6 cut(s) 118, 677, 708, 1056, 2630, 2753
BccI CCATC 6 cut(s) 68, 266, 403, 455, 1420, 2108
BcgI CGANNNNNNTGC 2 cut(s) 2046, 2080
BciT130I CCWGG 6 cut(s) 148, 250, 365, 837, 1168, 3226
BciVI GTATCC 3 cut(s) 2132, 2584, 2816
BclI TGATCA 2 cut(s) 2929, 2995
BcnI CCSGG 1 cut(s) 3083
BcoDI GTCTC 7 cut(s) 89, 990, 1491, 1951, 1963, 2345, 2356
BfaI CTAG 5 cut(s) 917, 1448, 2630, 2879, 3216
BfmI CTRYAG 7 cut(s) 428, 669, 1430, 1717, 2433, 2767, 3201
BfrI CTTAAG 1 cut(s) 710
BfuAI ACCTGC 2 cut(s) 2091, 2780
BfuI GTATCC 3 cut(s) 2132, 2584, 2816
BglII AGATCT 1 cut(s) 2008
BisI GCNGC 6 cut(s) 132, 666, 697, 1045, 2619, 2767
BlpI GCTNAGC 1 cut(s) 2548
BlsI GCNGC 6 cut(s) 133, 667, 698, 1046, 2620, 2768
BmcAI AGTACT 2 cut(s) 1923, 1993
Bme1390I CCNGG 7 cut(s) 148, 250, 365, 837, 1168, 3083, 3226
Bme18I GGWCC 2 cut(s) 2422, 2884
BmgBI CACGTC 1 cut(s) 2854
BmgT120I GGNCC 4 cut(s) 1838, 2422, 2702, 2884
BmiI GGNNCC 7 cut(s) 254, 978, 1427, 1693, 1840, 2423, 2901
BmrFI CCNGG 7 cut(s) 148, 250, 365, 837, 1168, 3083, 3226
BmrI ACTGGG 5 cut(s) 1482, 1934, 1983, 2596, 2621
BmsI GCATC 2 cut(s) 912, 2058
BmuI ACTGGG 5 cut(s) 1482, 1934, 1983, 2596, 2621
BplI GAGNNNNNCTC 4 cut(s) 71, 103, 2859, 2891
BpmI CTGGAG 3 cut(s) 2742, 2796, 3208
Bpu10I CCTNAGC 2 cut(s) 127, 2329
Bpu1102I GCTNAGC 1 cut(s) 2548
BpuEI CTTGAG 1 cut(s) 1271
BpuMI CCSGG 1 cut(s) 3083
Bsa29I ATCGAT 1 cut(s) 2456
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 8 cut(s) 363, 401, 456, 655, 1183, 1381, 1543, 3175
BsaXI ACNNNNNCTCC 4 cut(s) 1233, 1263, 1931, 1961
Bsc4I CCNNNNNNNGG 9 cut(s) 773, 1061, 1133, 1422, 1493, 1516, 1720, 2819, 3245
Bse3DI GCAATG 4 cut(s) 474, 2370, 2805, 3123
BseBI CCWGG 6 cut(s) 148, 250, 365, 837, 1168, 3226
BseCI ATCGAT 1 cut(s) 2456
BseDI CCNNGG 8 cut(s) 363, 401, 456, 655, 1183, 1381, 1543, 3175
BseGI GGATG 4 cut(s) 258, 466, 903, 1412
BseLI CCNNNNNNNGG 9 cut(s) 773, 1061, 1133, 1422, 1493, 1516, 1720, 2819, 3245
BseMI GCAATG 4 cut(s) 474, 2370, 2805, 3123
BseRI GAGGAG 2 cut(s) 1025, 1241
BseXI GCAGC 6 cut(s) 118, 677, 708, 1056, 2630, 2753
BsgI GTGCAG 1 cut(s) 606
BshFI GGCC 9 cut(s) 660, 1208, 1839, 2392, 2628, 2704, 2736, 3094, 3174
BshVI ATCGAT 1 cut(s) 2456
BsiHKAI GWGCWC 2 cut(s) 1542, 2877
BsiSI CCGG 1 cut(s) 3083
BslFI GGGAC 4 cut(s) 383, 657, 2435, 3034
BslI CCNNNNNNNGG 9 cut(s) 773, 1061, 1133, 1422, 1493, 1516, 1720, 2819, 3245
BsmAI GTCTC 7 cut(s) 89, 990, 1491, 1951, 1963, 2345, 2356
BsmBI CGTCTC 1 cut(s) 1951
BsmFI GGGAC 4 cut(s) 383, 657, 2435, 3034
BsmI GAATGC 1 cut(s) 2768
BsnI GGCC 9 cut(s) 660, 1208, 1839, 2392, 2628, 2704, 2736, 3094, 3174
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 4 cut(s) 571, 1542, 1800, 2877
Bsp1720I GCTNAGC 1 cut(s) 2548
BspACI CCGC 4 cut(s) 203, 591, 1052, 1372
BspANI GGCC 9 cut(s) 660, 1208, 1839, 2392, 2628, 2704, 2736, 3094, 3174
BspDI ATCGAT 1 cut(s) 2456
BspHI TCATGA 1 cut(s) 2644
BspLI GGNNCC 7 cut(s) 254, 978, 1427, 1693, 1840, 2423, 2901
BspMAI CTGCAG 4 cut(s) 432, 1434, 2771, 3205
BspMI ACCTGC 2 cut(s) 2091, 2780
BspPI GGATC 9 cut(s) 161, 375, 1242, 1267, 1363, 1393, 1893, 2461, 2843
BspQI GCTCTTC 1 cut(s) 3066
BspTI CTTAAG 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 89
BsrDI GCAATG 4 cut(s) 474, 2370, 2805, 3123
BssECI CCNNGG 8 cut(s) 363, 401, 456, 655, 1183, 1381, 1543, 3175
BssSI CACGAG 1 cut(s) 2039
BssT1I CCWWGG 4 cut(s) 456, 655, 1183, 3175
Bst2BI CACGAG 1 cut(s) 2039
Bst2UI CCWGG 6 cut(s) 148, 250, 365, 837, 1168, 3226
Bst6I CTCTTC 5 cut(s) 124, 862, 2564, 2724, 3066
BstAFI CTTAAG 1 cut(s) 710
BstC8I GCNNGC 4 cut(s) 589, 822, 1206, 2160
BstDSI CCRYGG 1 cut(s) 401
BstENI CCTNNNNNAGG 1 cut(s) 1491
BstF5I GGATG 4 cut(s) 258, 466, 903, 1412
BstMAI GTCTC 7 cut(s) 89, 990, 1491, 1951, 1963, 2345, 2356
BstMWI GCNNNNNNNGC 4 cut(s) 65, 100, 1177, 2168
BstNI CCWGG 6 cut(s) 148, 250, 365, 837, 1168, 3226
BstSCI CCNGG 7 cut(s) 146, 248, 363, 835, 1166, 3081, 3224
BstSFI CTRYAG 7 cut(s) 428, 669, 1430, 1717, 2433, 2767, 3201
BstV1I GCAGC 6 cut(s) 118, 677, 708, 1056, 2630, 2753
BstX2I RGATCY 4 cut(s) 367, 1234, 1885, 2008
BstXI CCANNNNNNTGG 3 cut(s) 177, 533, 824
BstYI RGATCY 4 cut(s) 367, 1234, 1885, 2008
Bsu15I ATCGAT 1 cut(s) 2456
BsuI GTATCC 3 cut(s) 2132, 2584, 2816
BsuRI GGCC 9 cut(s) 660, 1208, 1839, 2392, 2628, 2704, 2736, 3094, 3174
BsuTUI ATCGAT 1 cut(s) 2456
BtgI CCRYGG 1 cut(s) 401
BtrI CACGTC 1 cut(s) 2854
BtsCI GGATG 4 cut(s) 258, 466, 903, 1412
BtsI GCAGTG 1 cut(s) 2382
BtsIMutI CAGTG 5 cut(s) 322, 440, 1261, 1269, 2382
BveI ACCTGC 2 cut(s) 2091, 2780
Cac8I GCNNGC 4 cut(s) 589, 822, 1206, 2160
CaiI CAGNNNCTG 2 cut(s) 1628, 2187
CciI TCATGA 1 cut(s) 2644
Cfr13I GGNCC 4 cut(s) 1838, 2422, 2702, 2884
ClaI ATCGAT 1 cut(s) 2456
CsiI ACCWGGT 1 cut(s) 248
Csp6I GTAC 8 cut(s) 517, 1116, 1359, 1922, 1992, 2599, 2609, 2803
CspCI CAANNNNNGTGG 4 cut(s) 298, 333, 981, 1016
CviAII CATG 9 cut(s) 99, 171, 1529, 1603, 2087, 2564, 2624, 2645, 3137
CviQI GTAC 8 cut(s) 517, 1116, 1359, 1922, 1992, 2599, 2609, 2803
DraI TTTAAA 1 cut(s) 1080
DrdI GACNNNNNNGTC 1 cut(s) 1943
DseDI GACNNNNNNGTC 1 cut(s) 1943
EaeI YGGCCR 3 cut(s) 1206, 2390, 3172
Eam1104I CTCTTC 5 cut(s) 124, 862, 2564, 2724, 3066
EarI CTCTTC 5 cut(s) 124, 862, 2564, 2724, 3066
Ecl136II GAGCTC 1 cut(s) 1540
Eco130I CCWWGG 4 cut(s) 456, 655, 1183, 3175
Eco24I GRGCYC 3 cut(s) 571, 1542, 1800
Eco31I GGTCTC 1 cut(s) 89
Eco32I GATATC 3 cut(s) 630, 1038, 2108
Eco47I GGWCC 2 cut(s) 2422, 2884
Eco53kI GAGCTC 1 cut(s) 1540
Eco57I CTGAAG 1 cut(s) 219
EcoICRI GAGCTC 1 cut(s) 1540
EcoNI CCTNNNNNAGG 1 cut(s) 1491
EcoO109I RGGNCCY 3 cut(s) 1838, 2422, 2884
EcoRI GAATTC 3 cut(s) 445, 1825, 2978
EcoRII CCWGG 6 cut(s) 146, 248, 363, 835, 1166, 3224
EcoRV GATATC 3 cut(s) 630, 1038, 2108
EcoT14I CCWWGG 4 cut(s) 456, 655, 1183, 3175
EcoT22I ATGCAT 1 cut(s) 2051
EcoT38I GRGCYC 3 cut(s) 571, 1542, 1800
ErhI CCWWGG 4 cut(s) 456, 655, 1183, 3175
Esp3I CGTCTC 1 cut(s) 1951
FaeI CATG 9 cut(s) 102, 174, 1532, 1606, 2090, 2567, 2627, 2648, 3140
FalI AAGNNNNNCTT 2 cut(s) 3024, 3056
FaqI GGGAC 4 cut(s) 383, 657, 2435, 3034
FatI CATG 9 cut(s) 98, 170, 1528, 1602, 2086, 2563, 2623, 2644, 3136
FauI CCCGC 2 cut(s) 196, 584
FbaI TGATCA 2 cut(s) 2929, 2995
FblI GTMKAC 1 cut(s) 1981
Fnu4HI GCNGC 6 cut(s) 132, 666, 697, 1045, 2619, 2767
FokI GGATG 4 cut(s) 245, 473, 890, 1399
FriOI GRGCYC 3 cut(s) 571, 1542, 1800
Fsp4HI GCNGC 6 cut(s) 132, 666, 697, 1045, 2619, 2767
FspBI CTAG 5 cut(s) 917, 1448, 2630, 2879, 3216
GluI GCNGC 6 cut(s) 132, 666, 697, 1045, 2619, 2767
GsuI CTGGAG 3 cut(s) 2742, 2796, 3208
HaeIII GGCC 9 cut(s) 660, 1208, 1839, 2392, 2628, 2704, 2736, 3094, 3174
HapII CCGG 1 cut(s) 3083
Hin1II CATG 9 cut(s) 102, 174, 1532, 1606, 2090, 2567, 2627, 2648, 3140
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 1852, 2261
HpaII CCGG 1 cut(s) 3083
Hpy166II GTNNAC 5 cut(s) 6, 842, 1982, 3208, 3251
Hpy8I GTNNAC 5 cut(s) 6, 842, 1982, 3208, 3251
HpyAV CCTTC 7 cut(s) 121, 1566, 1610, 2135, 2314, 2890, 3264
HpyCH4IV ACGT 2 cut(s) 396, 2853
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 100, 1177, 2168
HpySE526I ACGT 2 cut(s) 396, 2853
Hsp92II CATG 9 cut(s) 102, 174, 1532, 1606, 2090, 2567, 2627, 2648, 3140
Ksp22I TGATCA 2 cut(s) 2929, 2995
LguI GCTCTTC 1 cut(s) 3066
Lsp1109I GCAGC 6 cut(s) 118, 677, 708, 1056, 2630, 2753
LweI GCATC 2 cut(s) 912, 2058
MabI ACCWGGT 1 cut(s) 248
MaeI CTAG 5 cut(s) 917, 1448, 2630, 2879, 3216
MaeII ACGT 2 cut(s) 396, 2853
MaeIII GTNAC 9 cut(s) 392, 776, 1352, 1748, 2287, 2849, 2938, 3109, 3184
MfeI CAATTG 2 cut(s) 336, 2988
MflI RGATCY 4 cut(s) 367, 1234, 1885, 2008
MhlI GDGCHC 4 cut(s) 571, 1542, 1800, 2877
MlsI TGGCCA 1 cut(s) 1208
MluNI TGGCCA 1 cut(s) 1208
MlyI GAGTC 3 cut(s) 2193, 2297, 2347
MmeI TCCRAC 5 cut(s) 179, 1364, 1716, 2381, 2802
Mox20I TGGCCA 1 cut(s) 1208
Mph1103I ATGCAT 1 cut(s) 2051
MroXI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
MscI TGGCCA 1 cut(s) 1208
MseI TTAA 9 cut(s) 276, 711, 1079, 1295, 1584, 2277, 2679, 2949, 3072
MslI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 2649, 3141
Msp20I TGGCCA 1 cut(s) 1208
MspA1I CMGCKG 1 cut(s) 131
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 1 cut(s) 3083
MspR9I CCNGG 7 cut(s) 148, 250, 365, 837, 1168, 3083, 3226
MunI CAATTG 2 cut(s) 336, 2988
Mva1269I GAATGC 1 cut(s) 2768
MvaI CCWGG 6 cut(s) 148, 250, 365, 837, 1168, 3226
MwoI GCNNNNNNNGC 4 cut(s) 65, 100, 1177, 2168
NciI CCSGG 1 cut(s) 3083
NlaIII CATG 9 cut(s) 102, 174, 1532, 1606, 2090, 2567, 2627, 2648, 3140
NlaIV GGNNCC 7 cut(s) 254, 978, 1427, 1693, 1840, 2423, 2901
NmeAIII GCCGAG 2 cut(s) 1293, 3150
NmuCI GTSAC 4 cut(s) 2287, 2849, 2938, 3184
NsiI ATGCAT 1 cut(s) 2051
PagI TCATGA 1 cut(s) 2644
PciSI GCTCTTC 1 cut(s) 3066
PctI GAATGC 1 cut(s) 2768
PdmI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
PflMI CCANNNNNTGG 2 cut(s) 773, 2819
PfoI TCCNGGA 1 cut(s) 146
PkrI GCNGC 6 cut(s) 133, 667, 698, 1046, 2620, 2768
PleI GAGTC 3 cut(s) 2192, 2297, 2347
PpsI GAGTC 3 cut(s) 2192, 2297, 2347
PpuMI RGGWCCY 2 cut(s) 2422, 2884
Psp124BI GAGCTC 1 cut(s) 1542
Psp5II RGGWCCY 2 cut(s) 2422, 2884
Psp6I CCWGG 6 cut(s) 146, 248, 363, 835, 1166, 3224
PspGI CCWGG 6 cut(s) 146, 248, 363, 835, 1166, 3224
PspN4I GGNNCC 7 cut(s) 254, 978, 1427, 1693, 1840, 2423, 2901
PspPI GGNCC 4 cut(s) 1838, 2422, 2702, 2884
PspPPI RGGWCCY 2 cut(s) 2422, 2884
PsrI GAACNNNNNNTAC 2 cut(s) 1914, 1946
PstI CTGCAG 4 cut(s) 432, 1434, 2771, 3205
PstNI CAGNNNCTG 2 cut(s) 1628, 2187
PsuI RGATCY 4 cut(s) 367, 1234, 1885, 2008
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 8 cut(s) 518, 1117, 1360, 1923, 1993, 2600, 2610, 2804
RsaNI GTAC 8 cut(s) 517, 1116, 1359, 1922, 1992, 2599, 2609, 2803
RseI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 2649, 3141
SacI GAGCTC 1 cut(s) 1542
SapI GCTCTTC 1 cut(s) 3066
SaqAI TTAA 9 cut(s) 276, 711, 1079, 1295, 1584, 2277, 2679, 2949, 3072
SatI GCNGC 6 cut(s) 132, 666, 697, 1045, 2619, 2767
Sau96I GGNCC 4 cut(s) 1838, 2422, 2702, 2884
ScaI AGTACT 2 cut(s) 1923, 1993
SchI GAGTC 3 cut(s) 2193, 2297, 2347
ScrFI CCNGG 7 cut(s) 148, 250, 365, 837, 1168, 3083, 3226
SduI GDGCHC 4 cut(s) 571, 1542, 1800, 2877
SexAI ACCWGGT 1 cut(s) 248
SfaNI GCATC 2 cut(s) 912, 2058
SfcI CTRYAG 7 cut(s) 428, 669, 1430, 1717, 2433, 2767, 3201
SinI GGWCC 2 cut(s) 2422, 2884
SmiMI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 2649, 3141
SmlI CTYRAG 2 cut(s) 710, 1286
SmoI CTYRAG 2 cut(s) 710, 1286
SsiI CCGC 4 cut(s) 203, 591, 1052, 1372
SspI AATATT 1 cut(s) 1348
SspMI CTAG 5 cut(s) 917, 1448, 2630, 2879, 3216
SstI GAGCTC 1 cut(s) 1542
StyD4I CCNGG 7 cut(s) 146, 248, 363, 835, 1166, 3081, 3224
StyI CCWWGG 4 cut(s) 456, 655, 1183, 3175
TaiI ACGT 2 cut(s) 399, 2856
TaqI TCGA 5 cut(s) 945, 1800, 2306, 2456, 2671
TatI WGTACW 3 cut(s) 1921, 1991, 2608
Tru1I TTAA 9 cut(s) 276, 711, 1079, 1295, 1584, 2277, 2679, 2949, 3072
Tru9I TTAA 9 cut(s) 276, 711, 1079, 1295, 1584, 2277, 2679, 2949, 3072
TscAI CASTG 5 cut(s) 322, 447, 1261, 1269, 2382
TseFI GTSAC 4 cut(s) 2287, 2849, 2938, 3184
TseI GCWGC 6 cut(s) 131, 665, 696, 1044, 2618, 2766
Tsp45I GTSAC 4 cut(s) 2287, 2849, 2938, 3184
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 5 cut(s) 322, 447, 1261, 1269, 2382
Van91I CCANNNNNTGG 2 cut(s) 773, 2819
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 2422, 2884
XagI CCTNNNNNAGG 1 cut(s) 1491
XcmI CCANNNNNNNNNTGG 1 cut(s) 1574
XmiI GTMKAC 1 cut(s) 1981
XmnI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
XspI CTAG 5 cut(s) 917, 1448, 2630, 2879, 3216
ZrmI AGTACT 2 cut(s) 1923, 1993
Zsp2I ATGCAT 1 cut(s) 2051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.