FvH4_2g14690

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007963
Physical Location & Seq
Reverse (-)
12878734 .. 12880854
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14690.t2

Sequence Viewer

Length: 1182 bp
ATGCAAGCCTCCCTCTACCTGGACACCAAAGCCCTCGCATGGCATTCACGTGGCCACACCTCAACCCACCACTCATTCCAGCCAACACCCACCCTCAGATTCCGTCCAATGCCGCCAGTCTCCGCCGTGGCCACGTCGCCTCCACCTCTAACTCGGCAGAAGACTCACTCTTTGCCACCCGAGAAGGTTGAGCTCTTCAAGTCACTAGAGGGCTGGGCCTCCCAGACGATCCTCCCGTTTCTGAAGCCGGTCGAGGAATGCTGGCAGCCTAGTACGTTCTTGCCGGACCAGTCGCTTCCGAGAGAGGAGTTCATGGACCGGGTTCGGGCGCTTAGGGACCGGACGGATGGCTTGCCCGACGAGTACTTTGTGGTGCTCGTCGGGGATATGATCACCGAGGAGGCGTTGCCTACGTACCAGAGTATGATCAATGGGCTCGATGGGGTGAGAGACGAGACCGGAGCTAGCTCGAGCCCGTGGGCGCGGTGGACTCGGGCTTGGACGGCTGAGGAAAACCGCCATGGTGATCTGCTCAAAACCTATCTCTACTTGTCTGGTCGGGTTGATATGTTCAAGATCGAGAAGACTGTTCAGTACCTTATTGGAGTTGGCATGGAGCCAGGAACGGAGAATAACCCGTACTTGGGGTTCGTGTACACATCATTTCAAGAGCGAGCCACGTTCATATCGCACGGCAACACGGCTCGGCTGGCCAAAGACCGTGGCGATCCGGTGCTTGCGCGCATATGCGGCACCATTGCATCAGACGAGAGGCGCCACGAGTTTGCCTATTCCAAGATAGTGGAAAAGCTGCTTGAAGTGGATCCAACCGGAGCAATGCTCGCCATTGCGGGCATGATGAGGAAGAAGATCACAATGCCGGCCCATCTTATGTACGACGGGGAGGACCCCAAGCTGTTTGAGCACTTTGCGGCAGTGGCTCAACGGCTTCGGGTGTACACGGCACATGATTATGCAGACATTTTGGAGTTTTTGATCGGAAGGTGGAGGTTGGAGAAGTTGGAGGGATTGACGGGGGAGGGTAAGCGTGCACAGGACTTTGTGTGTAGCTTGGCGCCGAGGATAAGGAGGCTGGAAGAGAGGGCCGATGAGCGAGCTAGGAAGATGGAGCCACAAGGCGTCAAGTTTAGCTGGATTTATAATAAAGAAGTGTCGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

394

Amino Acids

44.88

Weight (kDa)

6.62

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 61 - 386 1.6e-152 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AccB1I GGYRCC 3 cut(s) 752, 774, 1075
AccII CGCG 2 cut(s) 484, 742
AciI CCGC 7 cut(s) 113, 123, 484, 517, 750, 851, 932
AclWI GGATC 4 cut(s) 223, 722, 818, 831
AcoI YGGCCR 3 cut(s) 52, 129, 711
AcuI CTGAAG 1 cut(s) 263
AcvI CACGTG 1 cut(s) 50
AcyI GRCGYC 3 cut(s) 775, 1076, 1140
AfaI GTAC 8 cut(s) 274, 365, 416, 596, 641, 656, 896, 959
AfiI CCNNNNNNNGG 5 cut(s) 19, 39, 325, 643, 644
AgsI TTSAA 4 cut(s) 199, 574, 668, 818
AjiI CACGTC 1 cut(s) 135
AjnI CCWGG 2 cut(s) 18, 619
AluBI AGCT 8 cut(s) 193, 464, 468, 811, 916, 1071, 1118, 1152
AluI AGCT 8 cut(s) 193, 464, 468, 811, 916, 1071, 1118, 1152
Alw21I GWGCWC 4 cut(s) 195, 378, 927, 1054
Alw26I GTCTC 3 cut(s) 124, 444, 449
Alw44I GTGCAC 1 cut(s) 1050
AlwI GGATC 4 cut(s) 223, 722, 818, 831
Ama87I CYCGRG 3 cut(s) 179, 469, 492
AoxI GGCC 6 cut(s) 52, 129, 216, 711, 882, 1104
ApaLI GTGCAC 1 cut(s) 1050
ApeKI GCWGC 2 cut(s) 265, 811
ArsI GACNNNNNNTTYG 4 cut(s) 154, 186, 350, 382
AspLEI GCGC 6 cut(s) 331, 484, 742, 744, 777, 1078
AspS9I GGNCC 7 cut(s) 216, 286, 316, 337, 883, 907, 1104
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 3 cut(s) 385, 457, 536
AsuNHI GCTAGC 1 cut(s) 464
AvaI CYCGRG 3 cut(s) 179, 469, 492
AvaII GGWCC 4 cut(s) 286, 316, 337, 907
BaeGI GKGCMC 1 cut(s) 1054
BalI TGGCCA 3 cut(s) 54, 131, 713
BamHI GGATCC 1 cut(s) 823
BanI GGYRCC 3 cut(s) 752, 774, 1075
BanII GRGCYC 3 cut(s) 195, 438, 476
BauI CACGAG 1 cut(s) 779
BbrPI CACGTG 1 cut(s) 50
BbsI GAAGAC 2 cut(s) 167, 590
Bbv12I GWGCWC 4 cut(s) 195, 378, 927, 1054
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 2 cut(s) 277, 798
BccI CCATC 4 cut(s) 341, 434, 894, 1120
BceAI ACGGC 6 cut(s) 110, 519, 709, 717, 962, 978
BciT130I CCWGG 2 cut(s) 20, 621
BclI TGATCA 2 cut(s) 390, 426
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 3 cut(s) 124, 444, 449
BfaI CTAG 4 cut(s) 206, 270, 465, 1119
BfoI RGCGCY 3 cut(s) 332, 778, 1079
BisI GCNGC 5 cut(s) 113, 266, 751, 812, 933
BlsI GCNGC 5 cut(s) 114, 267, 752, 813, 934
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 3 cut(s) 20, 320, 621
Bme18I GGWCC 4 cut(s) 286, 316, 337, 907
BmeT110I CYCGRG 3 cut(s) 179, 469, 492
BmgBI CACGTC 1 cut(s) 135
BmgT120I GGNCC 7 cut(s) 216, 286, 316, 337, 883, 907, 1104
BmiI GGNNCC 8 cut(s) 338, 618, 754, 776, 825, 909, 1077, 1131
BmrFI CCNGG 3 cut(s) 20, 320, 621
BmsI GCATC 1 cut(s) 770
BmtI GCTAGC 1 cut(s) 468
BpiI GAAGAC 2 cut(s) 167, 590
BplI GAGNNNNNCTC 2 cut(s) 825, 857
Bpu10I CCTNAGC 2 cut(s) 332, 507
BpuMI CCSGG 1 cut(s) 320
BsaAI YACGTR 2 cut(s) 50, 414
BsaHI GRCGYC 3 cut(s) 775, 1076, 1140
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 6 cut(s) 126, 396, 476, 520, 721, 1079
BsaWI WCCGGW 4 cut(s) 339, 458, 730, 830
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 39, 325, 643, 644
Bse118I RCCGGY 2 cut(s) 247, 880
Bse1I ACTGG 2 cut(s) 116, 289
Bse3DI GCAATG 3 cut(s) 756, 843, 846
BseBI CCWGG 2 cut(s) 20, 621
BseDI CCNNGG 6 cut(s) 126, 396, 476, 520, 721, 1079
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 5 cut(s) 19, 39, 325, 643, 644
BseMI GCAATG 3 cut(s) 756, 843, 846
BseMII CTCAG 2 cut(s) 109, 498
BseNI ACTGG 2 cut(s) 116, 289
BsePI GCGCGC 1 cut(s) 740
BseRI GAGGAG 2 cut(s) 320, 413
BseSI GKGCMC 1 cut(s) 1054
BseXI GCAGC 2 cut(s) 277, 798
BseYI CCCAGC 1 cut(s) 213
Bsh1236I CGCG 2 cut(s) 484, 742
Bsh1285I CGRYCG 1 cut(s) 252
BshFI GGCC 6 cut(s) 54, 131, 218, 713, 884, 1106
BshNI GGYRCC 3 cut(s) 752, 774, 1075
BsiEI CGRYCG 1 cut(s) 252
BsiHKAI GWGCWC 4 cut(s) 195, 378, 927, 1054
BsiHKCI CYCGRG 3 cut(s) 179, 469, 492
BsiSI CCGG 8 cut(s) 248, 284, 319, 340, 459, 731, 831, 881
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 5 cut(s) 19, 39, 325, 643, 644
BsmAI GTCTC 3 cut(s) 124, 444, 449
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 2 cut(s) 43, 263
BsnI GGCC 6 cut(s) 54, 131, 218, 713, 884, 1106
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 3 cut(s) 179, 469, 492
Bsp1286I GDGCHC 6 cut(s) 195, 378, 438, 476, 927, 1054
Bsp1407I TGTACA 2 cut(s) 654, 957
Bsp143I GATC 9 cut(s) 228, 390, 426, 526, 576, 727, 823, 870, 996
Bsp19I CCATGG 1 cut(s) 520
BspACI CCGC 7 cut(s) 113, 123, 484, 517, 750, 851, 932
BspANI GGCC 6 cut(s) 54, 131, 218, 713, 884, 1106
BspCNI CTCAG 2 cut(s) 108, 499
BspFNI CGCG 2 cut(s) 484, 742
BspLI GGNNCC 8 cut(s) 338, 618, 754, 776, 825, 909, 1077, 1131
BspOI GCTAGC 1 cut(s) 468
BspPI GGATC 4 cut(s) 223, 722, 818, 831
BspQI GCTCTTC 1 cut(s) 200
BspT107I GGYRCC 3 cut(s) 752, 774, 1075
BspTNI GGTCTC 1 cut(s) 449
BsrDI GCAATG 3 cut(s) 756, 843, 846
BsrFI RCCGGY 2 cut(s) 247, 880
BsrGI TGTACA 2 cut(s) 654, 957
BsrI ACTGG 2 cut(s) 116, 289
BssAI RCCGGY 2 cut(s) 247, 880
BssECI CCNNGG 6 cut(s) 126, 396, 476, 520, 721, 1079
BssHII GCGCGC 1 cut(s) 740
BssMI GATC 9 cut(s) 228, 390, 426, 526, 576, 727, 823, 870, 996
BssNI GRCGYC 3 cut(s) 775, 1076, 1140
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 520
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 2 cut(s) 20, 621
Bst4CI ACNGT 2 cut(s) 589, 722
Bst6I CTCTTC 2 cut(s) 200, 1092
BstACI GRCGYC 3 cut(s) 775, 1076, 1140
BstAUI TGTACA 2 cut(s) 654, 957
BstBAI YACGTR 2 cut(s) 50, 414
BstDEI CTNAG 3 cut(s) 95, 332, 507
BstDSI CCRYGG 4 cut(s) 126, 476, 520, 721
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 2 cut(s) 484, 742
BstH2I RGCGCY 3 cut(s) 332, 778, 1079
BstHHI GCGC 6 cut(s) 331, 484, 742, 744, 777, 1078
BstKTI GATC 9 cut(s) 231, 393, 429, 529, 579, 730, 826, 873, 999
BstMAI GTCTC 3 cut(s) 124, 444, 449
BstMBI GATC 9 cut(s) 228, 390, 426, 526, 576, 727, 823, 870, 996
BstMCI CGRYCG 1 cut(s) 252
BstMWI GCNNNNNNNGC 6 cut(s) 503, 710, 750, 842, 922, 938
BstNI CCWGG 2 cut(s) 20, 621
BstSCI CCNGG 3 cut(s) 18, 318, 619
BstSLI GKGCMC 1 cut(s) 1054
BstSNI TACGTA 1 cut(s) 414
BstUI CGCG 2 cut(s) 484, 742
BstV1I GCAGC 2 cut(s) 277, 798
BstV2I GAAGAC 2 cut(s) 167, 590
BstX2I RGATCY 1 cut(s) 823
BstXI CCANNNNNNTGG 1 cut(s) 802
BstYI RGATCY 1 cut(s) 823
BsuRI GGCC 6 cut(s) 54, 131, 218, 713, 884, 1106
BtgI CCRYGG 4 cut(s) 126, 476, 520, 721
BtrI CACGTC 1 cut(s) 135
BtsCI GGATG 1 cut(s) 352
BtsI GCAGTG 1 cut(s) 942
BtsIMutI CAGTG 1 cut(s) 942
CfoI GCGC 6 cut(s) 331, 484, 742, 744, 777, 1078
Cfr10I RCCGGY 2 cut(s) 247, 880
Cfr13I GGNCC 7 cut(s) 216, 286, 316, 337, 883, 907, 1104
CseI GACGC 1 cut(s) 1129
Csp6I GTAC 8 cut(s) 273, 364, 415, 595, 640, 655, 895, 958
CspCI CAANNNNNGTGG 2 cut(s) 703, 738
CviAII CATG 6 cut(s) 39, 313, 521, 613, 856, 968
CviQI GTAC 8 cut(s) 273, 364, 415, 595, 640, 655, 895, 958
DdeI CTNAG 3 cut(s) 95, 332, 507
DinI GGCGCC 2 cut(s) 776, 1077
DpnI GATC 9 cut(s) 230, 392, 428, 528, 578, 729, 825, 872, 998
DpnII GATC 9 cut(s) 228, 390, 426, 526, 576, 727, 823, 870, 996
EaeI YGGCCR 3 cut(s) 52, 129, 711
Eam1104I CTCTTC 2 cut(s) 200, 1092
EarI CTCTTC 2 cut(s) 200, 1092
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 193
Eco105I TACGTA 1 cut(s) 414
Eco130I CCWWGG 1 cut(s) 520
Eco24I GRGCYC 3 cut(s) 195, 438, 476
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 4 cut(s) 286, 316, 337, 907
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 263
Eco72I CACGTG 1 cut(s) 50
Eco88I CYCGRG 3 cut(s) 179, 469, 492
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 2 cut(s) 18, 619
EcoT14I CCWWGG 1 cut(s) 520
EcoT38I GRGCYC 3 cut(s) 195, 438, 476
EgeI GGCGCC 2 cut(s) 776, 1077
EheI GGCGCC 2 cut(s) 776, 1077
ErhI CCWWGG 1 cut(s) 520
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 6 cut(s) 42, 316, 524, 616, 859, 971
FaqI GGGAC 1 cut(s) 350
FatI CATG 6 cut(s) 38, 312, 520, 612, 855, 967
FauI CCCGC 1 cut(s) 844
FauNDI CATATG 1 cut(s) 746
FbaI TGATCA 2 cut(s) 390, 426
Fnu4HI GCNGC 5 cut(s) 113, 266, 751, 812, 933
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 3 cut(s) 195, 438, 476
Fsp4HI GCNGC 5 cut(s) 113, 266, 751, 812, 933
FspBI CTAG 4 cut(s) 206, 270, 465, 1119
GlaI GCGC 6 cut(s) 330, 483, 741, 743, 776, 1077
GluI GCNGC 5 cut(s) 113, 266, 751, 812, 933
GsaI CCCAGC 1 cut(s) 217
HaeII RGCGCY 3 cut(s) 332, 778, 1079
HaeIII GGCC 6 cut(s) 54, 131, 218, 713, 884, 1106
HapII CCGG 8 cut(s) 248, 284, 319, 340, 459, 731, 831, 881
HgaI GACGC 1 cut(s) 1129
HhaI GCGC 6 cut(s) 331, 484, 742, 744, 777, 1078
Hin1I GRCGYC 3 cut(s) 775, 1076, 1140
Hin1II CATG 6 cut(s) 42, 316, 524, 616, 859, 971
Hin6I GCGC 6 cut(s) 329, 482, 740, 742, 775, 1076
HinP1I GCGC 6 cut(s) 329, 482, 740, 742, 775, 1076
HinfI GANTC 3 cut(s) 99, 163, 490
HpaII CCGG 8 cut(s) 248, 284, 319, 340, 459, 731, 831, 881
HphI GGTGA 3 cut(s) 385, 457, 536
Hpy166II GTNNAC 6 cut(s) 489, 655, 657, 958, 960, 1052
Hpy188I TCNGA 5 cut(s) 98, 243, 300, 766, 1001
Hpy188III TCNNGA 3 cut(s) 574, 580, 668
Hpy8I GTNNAC 6 cut(s) 489, 655, 657, 958, 960, 1052
Hpy99I CGWCG 4 cut(s) 139, 362, 383, 902
HpyAV CCTTC 2 cut(s) 178, 996
HpyCH4III ACNGT 2 cut(s) 589, 722
HpyCH4IV ACGT 5 cut(s) 49, 134, 275, 413, 680
HpyCH4V TGCA 4 cut(s) 4, 761, 977, 1052
HpyF10VI GCNNNNNNNGC 6 cut(s) 503, 710, 750, 842, 922, 938
HpyF3I CTNAG 3 cut(s) 95, 332, 507
HpySE526I ACGT 5 cut(s) 49, 134, 275, 413, 680
Hsp92I GRCGYC 3 cut(s) 775, 1076, 1140
Hsp92II CATG 6 cut(s) 42, 316, 524, 616, 859, 971
HspAI GCGC 6 cut(s) 329, 482, 740, 742, 775, 1076
KasI GGCGCC 2 cut(s) 774, 1075
KroI GCCGGC 1 cut(s) 880
KroNI GCCGGC 1 cut(s) 882
Ksp22I TGATCA 2 cut(s) 390, 426
Kzo9I GATC 9 cut(s) 228, 390, 426, 526, 576, 727, 823, 870, 996
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 4 cut(s) 461, 616, 833, 1129
Lsp1109I GCAGC 2 cut(s) 277, 798
LweI GCATC 1 cut(s) 770
MaeI CTAG 4 cut(s) 206, 270, 465, 1119
MaeII ACGT 5 cut(s) 49, 134, 275, 413, 680
MaeIII GTNAC 1 cut(s) 201
MalI GATC 9 cut(s) 230, 392, 428, 528, 578, 729, 825, 872, 998
MboI GATC 9 cut(s) 228, 390, 426, 526, 576, 727, 823, 870, 996
MboII GAAGA 7 cut(s) 172, 187, 595, 877, 880, 1109, 1135
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 6 cut(s) 195, 378, 438, 476, 927, 1054
MlsI TGGCCA 3 cut(s) 54, 131, 713
MluNI TGGCCA 3 cut(s) 54, 131, 713
Mly113I GGCGCC 2 cut(s) 775, 1076
MlyI GAGTC 2 cut(s) 157, 484
MmeI TCCRAC 3 cut(s) 851, 993, 1002
Mox20I TGGCCA 3 cut(s) 54, 131, 713
MroNI GCCGGC 1 cut(s) 880
MscI TGGCCA 3 cut(s) 54, 131, 713
MslI CAYNNNNRTG 2 cut(s) 48, 972
Msp20I TGGCCA 3 cut(s) 54, 131, 713
MspI CCGG 8 cut(s) 248, 284, 319, 340, 459, 731, 831, 881
MspR9I CCNGG 3 cut(s) 20, 320, 621
Mva1269I GAATGC 2 cut(s) 43, 263
MvaI CCWGG 2 cut(s) 20, 621
MvnI CGCG 2 cut(s) 484, 742
MwoI GCNNNNNNNGC 6 cut(s) 503, 710, 750, 842, 922, 938
NaeI GCCGGC 1 cut(s) 882
NarI GGCGCC 2 cut(s) 775, 1076
NciI CCSGG 1 cut(s) 320
NcoI CCATGG 1 cut(s) 520
NdeI CATATG 1 cut(s) 746
NdeII GATC 9 cut(s) 228, 390, 426, 526, 576, 727, 823, 870, 996
NgoMIV GCCGGC 1 cut(s) 880
NheI GCTAGC 1 cut(s) 464
NlaIII CATG 6 cut(s) 42, 316, 524, 616, 859, 971
NlaIV GGNNCC 8 cut(s) 338, 618, 754, 776, 825, 909, 1077, 1131
NmeAIII GCCGAG 3 cut(s) 133, 685, 1104
NmuCI GTSAC 1 cut(s) 201
PaeR7I CTCGAG 1 cut(s) 469
PauI GCGCGC 1 cut(s) 740
PciSI GCTCTTC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PctI GAATGC 2 cut(s) 43, 263
PdiI GCCGGC 1 cut(s) 882
PfeI GAWTC 1 cut(s) 99
PkrI GCNGC 5 cut(s) 114, 267, 752, 813, 934
PleI GAGTC 2 cut(s) 157, 484
PluTI GGCGCC 2 cut(s) 778, 1079
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 2 cut(s) 157, 484
Ppu21I YACGTR 2 cut(s) 50, 414
PpuMI RGGWCCY 1 cut(s) 907
PsiI TTATAA 1 cut(s) 1161
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 2 cut(s) 18, 619
PspCI CACGTG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 2 cut(s) 18, 619
PspN4I GGNNCC 8 cut(s) 338, 618, 754, 776, 825, 909, 1077, 1131
PspPI GGNCC 7 cut(s) 216, 286, 316, 337, 883, 907, 1104
PspPPI RGGWCCY 1 cut(s) 907
PspXI VCTCGAGB 1 cut(s) 469
PsrI GAACNNNNNNTAC 2 cut(s) 632, 664
PsuI RGATCY 1 cut(s) 823
PteI GCGCGC 1 cut(s) 740
RsaI GTAC 8 cut(s) 274, 365, 416, 596, 641, 656, 896, 959
RsaNI GTAC 8 cut(s) 273, 364, 415, 595, 640, 655, 895, 958
RseI CAYNNNNRTG 2 cut(s) 48, 972
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 200
SatI GCNGC 5 cut(s) 113, 266, 751, 812, 933
Sau3AI GATC 9 cut(s) 228, 390, 426, 526, 576, 727, 823, 870, 996
Sau96I GGNCC 7 cut(s) 216, 286, 316, 337, 883, 907, 1104
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 2 cut(s) 157, 484
ScrFI CCNGG 3 cut(s) 20, 320, 621
SduI GDGCHC 6 cut(s) 195, 378, 438, 476, 927, 1054
SfaNI GCATC 1 cut(s) 770
SfoI GGCGCC 2 cut(s) 776, 1077
Sfr274I CTCGAG 1 cut(s) 469
SinI GGWCC 4 cut(s) 286, 316, 337, 907
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 2 cut(s) 48, 972
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
SnaBI TACGTA 1 cut(s) 414
SsiI CCGC 7 cut(s) 113, 123, 484, 517, 750, 851, 932
SspDI GGCGCC 2 cut(s) 774, 1075
SspMI CTAG 4 cut(s) 206, 270, 465, 1119
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 3 cut(s) 18, 318, 619
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 2 cut(s) 589, 722
TaiI ACGT 5 cut(s) 52, 137, 278, 416, 683
TaqI TCGA 4 cut(s) 252, 438, 470, 579
TatI WGTACW 3 cut(s) 363, 654, 957
TauI GCSGC 3 cut(s) 115, 753, 935
TfiI GAWTC 1 cut(s) 99
TscAI CASTG 1 cut(s) 942
TseFI GTSAC 1 cut(s) 201
TseI GCWGC 2 cut(s) 265, 811
Tsp45I GTSAC 1 cut(s) 201
TspDTI ATGAA 2 cut(s) 301, 673
TspGWI ACGGA 3 cut(s) 92, 359, 641
TspRI CASTG 1 cut(s) 942
VneI GTGCAC 1 cut(s) 1050
VpaK11BI GGWCC 4 cut(s) 286, 316, 337, 907
XhoI CTCGAG 1 cut(s) 469
XspI CTAG 4 cut(s) 206, 270, 465, 1119
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.