pycom10g00910

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007963
Physical Location & Seq
Forward (+)
986166 .. 987827
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00910.1

Sequence Viewer

Length: 1296 bp
ATGTGTCACATTTTTCAAATTGCTTACCTAGTCCGAACAAAGATATCGTTTGTCGACAACATAGAACAAAGTCTGACAAAGGCAGTGCAAAAGAAACAAATTCGAGCCATGCAGACCTTGCTCTACATTGACACCAACACTCTTGCAAGGCCTCCACTTGGCCACACCCCAACCCACCACCGCCTCACTCCACCAAACCCCACCATCAAATTCCGATCAACACCCAGAGTCTCCGCCGTGGCCACAACTTCCCCGACACCTCTAACTCTGCAGAAGGCCCACTCACTGCCGCCTGAAAAAGTTGAGCTGTTCAAGTCGCTAGAGGGTTGGGCCGAGCAACACGTCCTCCCTTTCCTAAAACCGGTGGATCAGTGTTGGCAACCTAGCACCTTCTTGCCCGACTCATCACTTCCGGAAGAAGAGTTTCTGGATCAGGTTCGAGCCCTCCGTGATCGAACCAGCGAGCTCCCTGACGAGTACTTTGTGGCGCTCGTGGGGGACATGATTACTGAGGACGCGTTGCCCACTTATCAAACCATGGTAAATGGGCTAGACGGAGTGAAGGATGAGACTGGTTCTAGCCCGAGCGCGTGGGCTCGATGGAATCGGTCTTGGACGGCTGAGGAGAACAGACATGGGGATCTGCTCCGCACATATCTTTACTTGTCGGGTCGGGTCGATATGCTCAAGATCGACAGAACCGTGCAACATCTCATTGCAGCCGGCATGAATCCAGGGACAGAGAACAATCCCTACTTGGGGTTCGTGTACACGTCGTTCCAAGAGCGAGCCACGTTCGTATCGCACGGCAACACAGCTCGGCTCGCCAAAGAGGGCGGCGATCCAGTGCTCGCGCGCATTTGCGGCACCATTGCATCCGACGAAAGGCGCCACGAGATGGCCTACGCCAAGATCATTGAGAAGCTCCTCGAAGTGGACCCCAACGGAGCAATGGTGGCGATCGGCGACATGATGCGGAAGAAGATCACGATGCCGGCGCACCTTATGTACGATGGGGAAGACCCGAAGATATTCGAGCACTTCGCGGCGGTGGCCCAGCGGATCGGCGTGTACACGGCGGACGATTACGCGGATATCTTGGAGTTTTTGATCGGACGGTGGAAGTTGGAGAAGCTGGAGGGATTGGCGGCAGAAGGTGCTCGAGCACAGGATTTCGTGTGTGGGCTGGCACCGAGAATTAGAAGGCTTCAGGAACGGGCGGATGAGCAAGCACGGAAGATGGCGCCGCAGGCGGTTAAGTTTAGCTGGATCTTTAATAAAGAGTTGGCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

432

Amino Acids

48.68

Weight (kDa)

6.33

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 99 - 425 9.7e-153 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 866, 888, 1189, 1243
AccB7I CCANNNNNTGG 1 cut(s) 898
AccI GTMKAC 1 cut(s) 54
AccII CGCG 6 cut(s) 518, 590, 854, 856, 1046, 1091
AccIII TCCGGA 1 cut(s) 412
AclWI GGATC 6 cut(s) 375, 438, 648, 836, 1070, 1277
AcoI YGGCCR 2 cut(s) 160, 240
AcsI RAATTY 2 cut(s) 99, 209
AcuI CTGAAG 1 cut(s) 1193
AcyI GRCGYC 2 cut(s) 889, 1244
AfaI GTAC 4 cut(s) 479, 770, 1010, 1073
AfiI CCNNNNNNNGG 7 cut(s) 158, 361, 758, 759, 885, 898, 934
AflIII ACRYGT 3 cut(s) 340, 516, 771
AgeI ACCGGT 1 cut(s) 361
AgsI TTSAA 2 cut(s) 17, 313
AjiI CACGTC 2 cut(s) 343, 774
AjnI CCWGG 1 cut(s) 733
AluBI AGCT 6 cut(s) 307, 466, 818, 925, 1135, 1266
AluI AGCT 6 cut(s) 307, 466, 818, 925, 1135, 1266
Alw21I GWGCWC 5 cut(s) 468, 852, 1041, 1162, 1168
Alw26I GTCTC 2 cut(s) 235, 563
AlwI GGATC 6 cut(s) 375, 438, 648, 836, 1070, 1277
Ama87I CYCGRG 2 cut(s) 583, 1161
Aor13HI TCCGGA 1 cut(s) 412
AoxI GGCC 8 cut(s) 149, 160, 240, 276, 330, 900, 1053, 1287
ApeKI GCWGC 1 cut(s) 719
ApoI RAATTY 2 cut(s) 99, 209
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AsiGI ACCGGT 1 cut(s) 361
Asp700I GAANNNNTTC 2 cut(s) 423, 1031
AspLEI GCGC 7 cut(s) 490, 590, 856, 858, 891, 1000, 1246
AspS9I GGNCC 4 cut(s) 277, 330, 937, 1054
AvaI CYCGRG 2 cut(s) 583, 1161
AvaII GGWCC 1 cut(s) 937
BaeI ACNNNNGTAYC 2 cut(s) 783, 816
BalI TGGCCA 2 cut(s) 162, 242
BanI GGYRCC 4 cut(s) 866, 888, 1189, 1243
BanII GRGCYC 3 cut(s) 445, 468, 598
BauI CACGAG 2 cut(s) 491, 893
BbsI GAAGAC 1 cut(s) 1026
Bbv12I GWGCWC 5 cut(s) 468, 852, 1041, 1162, 1168
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 1 cut(s) 731
BccI CCATC 5 cut(s) 212, 594, 892, 1007, 1234
BceAI ACGGC 4 cut(s) 221, 633, 823, 1092
BciT130I CCWGG 1 cut(s) 735
BcoDI GTCTC 2 cut(s) 235, 563
BfaI CTAG 5 cut(s) 29, 320, 384, 551, 579
BfmI CTRYAG 1 cut(s) 269
BfoI RGCGCY 3 cut(s) 491, 892, 1247
BisI GCNGC 7 cut(s) 290, 720, 838, 865, 1047, 1149, 1247
BlsI GCNGC 7 cut(s) 291, 721, 839, 866, 1048, 1150, 1248
BmcAI AGTACT 1 cut(s) 479
Bme1390I CCNGG 1 cut(s) 735
Bme18I GGWCC 1 cut(s) 937
BmeT110I CYCGRG 2 cut(s) 583, 1161
BmgBI CACGTC 2 cut(s) 343, 774
BmgT120I GGNCC 4 cut(s) 277, 330, 937, 1054
BmiI GGNNCC 5 cut(s) 868, 890, 939, 1191, 1245
BmrFI CCNGG 1 cut(s) 735
BmsI GCATC 3 cut(s) 884, 963, 981
BpiI GAAGAC 1 cut(s) 1026
BpmI CTGGAG 1 cut(s) 1157
Bpu10I CCTNAGC 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 671
BsaHI GRCGYC 2 cut(s) 889, 1244
BsaJI CCNNGG 3 cut(s) 237, 537, 734
BsaWI WCCGGW 2 cut(s) 361, 412
BsaXI ACNNNNNCTCC 4 cut(s) 330, 360, 939, 969
Bsc4I CCNNNNNNNGG 7 cut(s) 158, 361, 758, 759, 885, 898, 934
Bse118I RCCGGY 3 cut(s) 361, 722, 994
Bse1I ACTGG 2 cut(s) 577, 845
Bse3DI GCAATG 3 cut(s) 714, 870, 957
BseAI TCCGGA 1 cut(s) 412
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 3 cut(s) 237, 537, 734
BseGI GGATG 3 cut(s) 571, 875, 1228
BseLI CCNNNNNNNGG 7 cut(s) 158, 361, 758, 759, 885, 898, 934
BseMI GCAATG 3 cut(s) 714, 870, 957
BseMII CTCAG 2 cut(s) 501, 612
BseNI ACTGG 2 cut(s) 577, 845
BsePI GCGCGC 1 cut(s) 854
BseRI GAGGAG 2 cut(s) 638, 917
BseXI GCAGC 1 cut(s) 731
BseYI CCCAGC 1 cut(s) 1056
Bsh1236I CGCG 6 cut(s) 518, 590, 854, 856, 1046, 1091
Bsh1285I CGRYCG 1 cut(s) 963
BshFI GGCC 8 cut(s) 151, 162, 242, 278, 332, 902, 1055, 1289
BshNI GGYRCC 4 cut(s) 866, 888, 1189, 1243
BshTI ACCGGT 1 cut(s) 361
BsiEI CGRYCG 1 cut(s) 963
BsiHKAI GWGCWC 5 cut(s) 468, 852, 1041, 1162, 1168
BsiHKCI CYCGRG 2 cut(s) 583, 1161
BsiSI CCGG 4 cut(s) 362, 413, 723, 995
BslFI GGGAC 2 cut(s) 512, 751
BslI CCNNNNNNNGG 7 cut(s) 158, 361, 758, 759, 885, 898, 934
BsmAI GTCTC 2 cut(s) 235, 563
BsmFI GGGAC 2 cut(s) 512, 751
BsnI GGCC 8 cut(s) 151, 162, 242, 278, 332, 902, 1055, 1289
BsoBI CYCGRG 2 cut(s) 583, 1161
Bsp1286I GDGCHC 7 cut(s) 445, 468, 598, 852, 1041, 1162, 1168
Bsp13I TCCGGA 1 cut(s) 412
Bsp1407I TGTACA 2 cut(s) 768, 1071
Bsp19I CCATGG 1 cut(s) 537
BspANI GGCC 8 cut(s) 151, 162, 242, 278, 332, 902, 1055, 1289
BspCNI CTCAG 2 cut(s) 502, 613
BspEI TCCGGA 1 cut(s) 412
BspFNI CGCG 6 cut(s) 518, 590, 854, 856, 1046, 1091
BspLI GGNNCC 5 cut(s) 868, 890, 939, 1191, 1245
BspMAI CTGCAG 1 cut(s) 273
BspPI GGATC 6 cut(s) 375, 438, 648, 836, 1070, 1277
BspT107I GGYRCC 4 cut(s) 866, 888, 1189, 1243
BsrDI GCAATG 3 cut(s) 714, 870, 957
BsrFI RCCGGY 3 cut(s) 361, 722, 994
BsrGI TGTACA 2 cut(s) 768, 1071
BsrI ACTGG 2 cut(s) 577, 845
BssAI RCCGGY 3 cut(s) 361, 722, 994
BssECI CCNNGG 3 cut(s) 237, 537, 734
BssHII GCGCGC 1 cut(s) 854
BssNI GRCGYC 2 cut(s) 889, 1244
BssSI CACGAG 2 cut(s) 491, 893
BssT1I CCWWGG 1 cut(s) 537
Bst2BI CACGAG 2 cut(s) 491, 893
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 2 cut(s) 703, 1119
Bst6I CTCTTC 1 cut(s) 414
BstACI GRCGYC 2 cut(s) 889, 1244
BstAPI GCANNNNNTGC 2 cut(s) 118, 1157
BstAUI TGTACA 2 cut(s) 768, 1071
BstDEI CTNAG 2 cut(s) 510, 621
BstDSI CCRYGG 2 cut(s) 237, 537
BstF5I GGATG 3 cut(s) 571, 875, 1228
BstFNI CGCG 6 cut(s) 518, 590, 854, 856, 1046, 1091
BstH2I RGCGCY 3 cut(s) 491, 892, 1247
BstHHI GCGC 7 cut(s) 490, 590, 856, 858, 891, 1000, 1246
BstMAI GTCTC 2 cut(s) 235, 563
BstMCI CGRYCG 1 cut(s) 963
BstMWI GCNNNNNNNGC 7 cut(s) 118, 824, 864, 956, 1052, 1157, 1250
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 1 cut(s) 733
BstSFI CTRYAG 1 cut(s) 269
BstUI CGCG 6 cut(s) 518, 590, 854, 856, 1046, 1091
BstV1I GCAGC 1 cut(s) 731
BstV2I GAAGAC 1 cut(s) 1026
BstX2I RGATCY 2 cut(s) 640, 1269
BstYI RGATCY 2 cut(s) 640, 1269
BsuRI GGCC 8 cut(s) 151, 162, 242, 278, 332, 902, 1055, 1289
BtgI CCRYGG 2 cut(s) 237, 537
BtrI CACGTC 2 cut(s) 343, 774
BtsCI GGATG 3 cut(s) 571, 875, 1228
BtsI GCAGTG 2 cut(s) 90, 284
BtsIMutI CAGTG 4 cut(s) 90, 284, 377, 852
CfoI GCGC 7 cut(s) 490, 590, 856, 858, 891, 1000, 1246
Cfr10I RCCGGY 3 cut(s) 361, 722, 994
Cfr13I GGNCC 4 cut(s) 277, 330, 937, 1054
CseI GACGC 1 cut(s) 524
Csp6I GTAC 4 cut(s) 478, 769, 1009, 1072
CspAI ACCGGT 1 cut(s) 361
CviAII CATG 6 cut(s) 109, 502, 538, 635, 727, 970
CviQI GTAC 4 cut(s) 478, 769, 1009, 1072
DdeI CTNAG 2 cut(s) 510, 621
DinI GGCGCC 2 cut(s) 890, 1245
EaeI YGGCCR 2 cut(s) 160, 240
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
EciI GGCGGA 3 cut(s) 223, 1094, 1235
Ecl136II GAGCTC 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 537
Eco147I AGGCCT 1 cut(s) 151
Eco24I GRGCYC 3 cut(s) 445, 468, 598
Eco32I GATATC 2 cut(s) 45, 1096
Eco47I GGWCC 1 cut(s) 937
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 1193
Eco88I CYCGRG 2 cut(s) 583, 1161
EcoICRI GAGCTC 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 733
EcoRV GATATC 2 cut(s) 45, 1096
EcoT14I CCWWGG 1 cut(s) 537
EcoT38I GRGCYC 3 cut(s) 445, 468, 598
EgeI GGCGCC 2 cut(s) 890, 1245
EheI GGCGCC 2 cut(s) 890, 1245
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 6 cut(s) 112, 505, 541, 638, 730, 973
FaqI GGGAC 2 cut(s) 512, 751
FatI CATG 6 cut(s) 108, 501, 537, 634, 726, 969
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 7 cut(s) 290, 720, 838, 865, 1047, 1149, 1247
FokI GGATG 3 cut(s) 578, 862, 1235
FriOI GRGCYC 3 cut(s) 445, 468, 598
Fsp4HI GCNGC 7 cut(s) 290, 720, 838, 865, 1047, 1149, 1247
FspBI CTAG 5 cut(s) 29, 320, 384, 551, 579
GlaI GCGC 7 cut(s) 489, 589, 855, 857, 890, 999, 1245
GluI GCNGC 7 cut(s) 290, 720, 838, 865, 1047, 1149, 1247
GsaI CCCAGC 1 cut(s) 1060
GsuI CTGGAG 1 cut(s) 1157
HaeII RGCGCY 3 cut(s) 491, 892, 1247
HaeIII GGCC 8 cut(s) 151, 162, 242, 278, 332, 902, 1055, 1289
HapII CCGG 4 cut(s) 362, 413, 723, 995
HgaI GACGC 1 cut(s) 524
HhaI GCGC 7 cut(s) 490, 590, 856, 858, 891, 1000, 1246
Hin1I GRCGYC 2 cut(s) 889, 1244
Hin1II CATG 6 cut(s) 112, 505, 541, 638, 730, 973
Hin6I GCGC 7 cut(s) 488, 588, 854, 856, 889, 998, 1244
HinP1I GCGC 7 cut(s) 488, 588, 854, 856, 889, 998, 1244
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 4 cut(s) 228, 401, 604, 730
HpaII CCGG 4 cut(s) 362, 413, 723, 995
Hpy166II GTNNAC 6 cut(s) 55, 769, 771, 937, 1072, 1074
Hpy188I TCNGA 5 cut(s) 35, 75, 215, 880, 1115
Hpy188III TCNNGA 5 cut(s) 413, 428, 688, 988, 1211
Hpy8I GTNNAC 6 cut(s) 55, 769, 771, 937, 1072, 1074
Hpy99I CGWCG 2 cut(s) 778, 884
HpyAV CCTTC 5 cut(s) 268, 400, 556, 1148, 1197
HpyCH4III ACNGT 2 cut(s) 703, 1119
HpyCH4IV ACGT 3 cut(s) 342, 773, 794
HpyCH4V TGCA 7 cut(s) 88, 112, 146, 271, 706, 719, 875
HpyF10VI GCNNNNNNNGC 7 cut(s) 118, 824, 864, 956, 1052, 1157, 1250
HpyF3I CTNAG 2 cut(s) 510, 621
HpySE526I ACGT 3 cut(s) 342, 773, 794
Hsp92I GRCGYC 2 cut(s) 889, 1244
Hsp92II CATG 6 cut(s) 112, 505, 541, 638, 730, 973
HspAI GCGC 7 cut(s) 488, 588, 854, 856, 889, 998, 1244
KasI GGCGCC 2 cut(s) 888, 1243
Kpn2I TCCGGA 1 cut(s) 412
KroI GCCGGC 2 cut(s) 722, 994
KroNI GCCGGC 2 cut(s) 724, 996
LmnI GCTCC 4 cut(s) 471, 651, 930, 947
Lsp1109I GCAGC 1 cut(s) 731
LweI GCATC 3 cut(s) 884, 963, 981
MaeI CTAG 5 cut(s) 29, 320, 384, 551, 579
MaeII ACGT 3 cut(s) 342, 773, 794
MaeIII GTNAC 1 cut(s) 5
MboII GAAGA 7 cut(s) 428, 431, 991, 994, 1031, 1039, 1249
MflI RGATCY 2 cut(s) 640, 1269
MhlI GDGCHC 7 cut(s) 445, 468, 598, 852, 1041, 1162, 1168
MlsI TGGCCA 2 cut(s) 162, 242
MluCI AATT 4 cut(s) 18, 99, 209, 1197
MluI ACGCGT 1 cut(s) 516
MluNI TGGCCA 2 cut(s) 162, 242
Mly113I GGCGCC 2 cut(s) 889, 1244
MlyI GAGTC 2 cut(s) 237, 395
MmeI TCCRAC 2 cut(s) 903, 1107
Mox20I TGGCCA 2 cut(s) 162, 242
MroI TCCGGA 1 cut(s) 412
MroNI GCCGGC 2 cut(s) 722, 994
MroXI GAANNNNTTC 2 cut(s) 423, 1031
MscI TGGCCA 2 cut(s) 162, 242
MseI TTAA 2 cut(s) 1257, 1275
Msp20I TGGCCA 2 cut(s) 162, 242
MspA1I CMGCKG 1 cut(s) 1060
MspI CCGG 4 cut(s) 362, 413, 723, 995
MspR9I CCNGG 1 cut(s) 735
MvaI CCWGG 1 cut(s) 735
MvnI CGCG 6 cut(s) 518, 590, 854, 856, 1046, 1091
MwoI GCNNNNNNNGC 7 cut(s) 118, 824, 864, 956, 1052, 1157, 1250
NaeI GCCGGC 2 cut(s) 724, 996
NarI GGCGCC 2 cut(s) 889, 1244
NcoI CCATGG 1 cut(s) 537
NgoMIV GCCGGC 2 cut(s) 722, 994
NlaIII CATG 6 cut(s) 112, 505, 541, 638, 730, 973
NlaIV GGNNCC 5 cut(s) 868, 890, 939, 1191, 1245
NmeAIII GCCGAG 2 cut(s) 358, 799
NmuCI GTSAC 1 cut(s) 5
PaeR7I CTCGAG 1 cut(s) 1161
PauI GCGCGC 1 cut(s) 854
PceI AGGCCT 1 cut(s) 151
PcsI WCGNNNNNNNCGW 2 cut(s) 445, 699
PdiI GCCGGC 2 cut(s) 724, 996
PdmI GAANNNNTTC 2 cut(s) 423, 1031
PfeI GAWTC 2 cut(s) 604, 730
PflMI CCANNNNNTGG 1 cut(s) 898
PinAI ACCGGT 1 cut(s) 361
PkrI GCNGC 7 cut(s) 291, 721, 839, 866, 1048, 1150, 1248
Ple19I CGATCG 1 cut(s) 963
PleI GAGTC 2 cut(s) 236, 395
PluTI GGCGCC 2 cut(s) 892, 1247
PpsI GAGTC 2 cut(s) 236, 395
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 733
PspFI CCCAGC 1 cut(s) 1056
PspGI CCWGG 1 cut(s) 733
PspN4I GGNNCC 5 cut(s) 868, 890, 939, 1191, 1245
PspPI GGNCC 4 cut(s) 277, 330, 937, 1054
PspXI VCTCGAGB 1 cut(s) 1161
PsrI GAACNNNNNNTAC 4 cut(s) 737, 761, 769, 793
PstI CTGCAG 1 cut(s) 273
PsuI RGATCY 2 cut(s) 640, 1269
PteI GCGCGC 1 cut(s) 854
PvuI CGATCG 1 cut(s) 963
RsaI GTAC 4 cut(s) 479, 770, 1010, 1073
RsaNI GTAC 4 cut(s) 478, 769, 1009, 1072
SacI GAGCTC 1 cut(s) 468
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 2 cut(s) 1257, 1275
SatI GCNGC 7 cut(s) 290, 720, 838, 865, 1047, 1149, 1247
Sau96I GGNCC 4 cut(s) 277, 330, 937, 1054
ScaI AGTACT 1 cut(s) 479
SchI GAGTC 2 cut(s) 237, 395
ScrFI CCNGG 1 cut(s) 735
SduI GDGCHC 7 cut(s) 445, 468, 598, 852, 1041, 1162, 1168
SfaNI GCATC 3 cut(s) 884, 963, 981
SfcI CTRYAG 1 cut(s) 269
SfoI GGCGCC 2 cut(s) 890, 1245
Sfr274I CTCGAG 1 cut(s) 1161
SinI GGWCC 1 cut(s) 937
SlaI CTCGAG 1 cut(s) 1161
SmlI CTYRAG 2 cut(s) 686, 1161
SmoI CTYRAG 2 cut(s) 686, 1161
Sse9I AATT 4 cut(s) 18, 99, 209, 1197
SseBI AGGCCT 1 cut(s) 151
SspDI GGCGCC 2 cut(s) 888, 1243
SspMI CTAG 5 cut(s) 29, 320, 384, 551, 579
SstI GAGCTC 1 cut(s) 468
StuI AGGCCT 1 cut(s) 151
StyD4I CCNGG 1 cut(s) 733
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 2 cut(s) 703, 1119
TaiI ACGT 3 cut(s) 345, 776, 797
TaqII GACCGA 1 cut(s) 597
TasI AATT 4 cut(s) 18, 99, 209, 1197
TatI WGTACW 3 cut(s) 477, 768, 1071
TauI GCSGC 6 cut(s) 292, 840, 867, 1049, 1151, 1249
TfiI GAWTC 2 cut(s) 604, 730
Tru1I TTAA 2 cut(s) 1257, 1275
Tru9I TTAA 2 cut(s) 1257, 1275
TscAI CASTG 4 cut(s) 90, 291, 377, 852
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 1 cut(s) 719
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 743
TspGWI ACGGA 4 cut(s) 437, 570, 960, 1249
TspRI CASTG 4 cut(s) 90, 291, 377, 852
Van91I CCANNNNNTGG 1 cut(s) 898
VpaK11BI GGWCC 1 cut(s) 937
XapI RAATTY 2 cut(s) 99, 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XhoI CTCGAG 1 cut(s) 1161
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 2 cut(s) 423, 1031
XspI CTAG 5 cut(s) 29, 320, 384, 551, 579
ZrmI AGTACT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.