MD10G1014800.v1.1

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007963
Physical Location & Seq
Forward (+)
1992710 .. 1994297
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1014800.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGCAGACCTCGCTCTACATTGACACCAAAACTCTTGCATGGCCTTCACTTGGCCACACCCCAACCCACCACCGCCTCACTCCACCAAACCCCACCATCAAATTCCGATCAACACCCACGGTCTCCGCCGTGGCCACAGCTTCCCCGCCACCTCTAACTCGGCAGAAGGCCCACTCACTGCCGCCTGAAAAAGTTGAGCTGTTCAAGTCGCTAGAGGGCTGGGCCGAGCAACACGTCCTCCCATTCCTAAAACCGGTGGATCAGTGCTGGCAGCCTAGCACCTTCTTGCCCGACTCATCACTTCCGGAAGAAGAGTTTCTGGATCAGGTTAGAGCCCTCCGTGATCGAACCGGCGAGCTCCCTGACGAGTACTTTGTGGCGCTCGTGGGGGACATGATTACTGAGGACGCGTTGCCCACTTATCAAACCATGGTAAATGGGCTAGATGGAGTGAAGGATGAGACTGGTTCTAGCCCGAGCGCGTGGGCTCGATGGAATCGGTCTTGGACGGCTGAGGAGAACAGACATGGGGATCTGCTCCGCACATATCTTTACTTGTCGGGTCGGGTCGATATGCTCAAGATCGACAGAACCGTACAACATCTCATTGGAGCCGGCATGAATCCAGGGACAGAGAACAATCCCTACTTGGGGTTCGTGTACACGTCATTCCAAGAGCGAGCCACGTTCGTATCGCACGGCAACACAGCTAGGCTCGCCAAAGAGGGCGGCGATCCAGTGCTCGCGCGCATTTGCGGCACCATTGCATCCGACGAAAGGCGCCACGAGATGGCCTACGCCAAGATCATTGAGAAGCTCCTCGAAGTGGATCCCACCGGAGCAATGGTGGCGATCGGCGACATGATGCGGAAGAAGATCACGATGCCGGCGCACCTTATGTACGATGGGGAAGACCCGAAGATATTCGAGCACTTTGCGGCGGTGGCTCAGCGGATCGGCGTGTACACGGCGGACGATTACGCGGATATCTTGGAGTTTTTGATCGGACGGTGGAATTTGGAGAAGCTGGAGGGATTGGCGGCGGAAGGTGCTCGAGCACAGGATTTCGTGTGTGGGCTGGCTCCGAGAATTAGAAGGCTTCAGGAACGGGCGGATGAGCAAGCACGGAAGATGGCGCCGCAGGCGGTTAAGTTTAGCTGGATATTTAATAAAGAGGTGGCCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

396

Amino Acids

44.35

Weight (kDa)

5.72

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 63 - 389 1.2e-151 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 758, 780, 1135
AccB7I CCANNNNNTGG 1 cut(s) 790
AccII CGCG 5 cut(s) 410, 482, 746, 748, 983
AccIII TCCGGA 1 cut(s) 304
AclWI GGATC 7 cut(s) 267, 330, 540, 728, 824, 837, 962
AcoI YGGCCR 2 cut(s) 52, 132
AcsI RAATTY 2 cut(s) 101, 1015
AcuI CTGAAG 1 cut(s) 1085
AcyI GRCGYC 2 cut(s) 781, 1136
AfaI GTAC 5 cut(s) 371, 597, 662, 902, 965
AfiI CCNNNNNNNGG 7 cut(s) 50, 253, 650, 651, 777, 790, 826
AflIII ACRYGT 3 cut(s) 232, 408, 663
AgeI ACCGGT 1 cut(s) 253
AgsI TTSAA 1 cut(s) 205
AjiI CACGTC 2 cut(s) 235, 666
AjnI CCWGG 1 cut(s) 625
AluBI AGCT 7 cut(s) 140, 199, 358, 710, 817, 1027, 1158
AluI AGCT 7 cut(s) 140, 199, 358, 710, 817, 1027, 1158
Alw21I GWGCWC 5 cut(s) 360, 744, 933, 1054, 1060
Alw26I GTCTC 2 cut(s) 127, 455
AlwI GGATC 7 cut(s) 267, 330, 540, 728, 824, 837, 962
Ama87I CYCGRG 2 cut(s) 475, 1053
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 7 cut(s) 41, 52, 132, 168, 222, 792, 1179
ApeKI GCWGC 1 cut(s) 271
ApoI RAATTY 2 cut(s) 101, 1015
ArsI GACNNNNNNTTYG 2 cut(s) 356, 388
AsiGI ACCGGT 1 cut(s) 253
Asp700I GAANNNNTTC 2 cut(s) 315, 923
AspLEI GCGC 7 cut(s) 382, 482, 748, 750, 783, 892, 1138
AspS9I GGNCC 2 cut(s) 169, 222
AvaI CYCGRG 2 cut(s) 475, 1053
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BalI TGGCCA 2 cut(s) 54, 134
BamHI GGATCC 1 cut(s) 829
BanI GGYRCC 3 cut(s) 758, 780, 1135
BanII GRGCYC 3 cut(s) 337, 360, 490
BauI CACGAG 2 cut(s) 383, 785
BbsI GAAGAC 1 cut(s) 918
Bbv12I GWGCWC 5 cut(s) 360, 744, 933, 1054, 1060
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 283
BccI CCATC 6 cut(s) 104, 440, 486, 784, 899, 1126
BceAI ACGGC 4 cut(s) 113, 525, 715, 984
BcgI CGANNNNNNTGC 2 cut(s) 917, 951
BciT130I CCWGG 1 cut(s) 627
BcoDI GTCTC 2 cut(s) 127, 455
BfaI CTAG 5 cut(s) 212, 276, 443, 471, 711
BfoI RGCGCY 3 cut(s) 383, 784, 1139
BisI GCNGC 7 cut(s) 182, 272, 730, 757, 939, 1041, 1139
BlpI GCTNAGC 1 cut(s) 948
BlsI GCNGC 7 cut(s) 183, 273, 731, 758, 940, 1042, 1140
BmcAI AGTACT 1 cut(s) 371
Bme1390I CCNGG 1 cut(s) 627
BmeT110I CYCGRG 2 cut(s) 475, 1053
BmgBI CACGTC 2 cut(s) 235, 666
BmgT120I GGNCC 2 cut(s) 169, 222
BmiI GGNNCC 6 cut(s) 613, 760, 782, 831, 1083, 1137
BmrFI CCNGG 1 cut(s) 627
BmsI GCATC 3 cut(s) 776, 855, 873
BpiI GAAGAC 1 cut(s) 918
BpmI CTGGAG 1 cut(s) 1049
Bpu10I CCTNAGC 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 948
BpuEI CTTGAG 1 cut(s) 563
BsaHI GRCGYC 2 cut(s) 781, 1136
BsaI GGTCTC 1 cut(s) 127
BsaJI CCNNGG 4 cut(s) 117, 129, 429, 626
BsaWI WCCGGW 3 cut(s) 253, 304, 836
BsaXI ACNNNNNCTCC 4 cut(s) 222, 252, 831, 861
Bsc4I CCNNNNNNNGG 7 cut(s) 50, 253, 650, 651, 777, 790, 826
Bse118I RCCGGY 4 cut(s) 253, 350, 614, 886
Bse1I ACTGG 2 cut(s) 469, 737
Bse3DI GCAATG 2 cut(s) 762, 849
BseAI TCCGGA 1 cut(s) 304
BseBI CCWGG 1 cut(s) 627
BseDI CCNNGG 4 cut(s) 117, 129, 429, 626
BseGI GGATG 3 cut(s) 463, 767, 1120
BseLI CCNNNNNNNGG 7 cut(s) 50, 253, 650, 651, 777, 790, 826
BseMI GCAATG 2 cut(s) 762, 849
BseMII CTCAG 3 cut(s) 393, 504, 962
BseNI ACTGG 2 cut(s) 469, 737
BsePI GCGCGC 1 cut(s) 746
BseRI GAGGAG 2 cut(s) 530, 809
BseXI GCAGC 1 cut(s) 283
BseYI CCCAGC 1 cut(s) 219
Bsh1236I CGCG 5 cut(s) 410, 482, 746, 748, 983
Bsh1285I CGRYCG 1 cut(s) 855
BshFI GGCC 7 cut(s) 43, 54, 134, 170, 224, 794, 1181
BshNI GGYRCC 3 cut(s) 758, 780, 1135
BshTI ACCGGT 1 cut(s) 253
BsiEI CGRYCG 1 cut(s) 855
BsiHKAI GWGCWC 5 cut(s) 360, 744, 933, 1054, 1060
BsiHKCI CYCGRG 2 cut(s) 475, 1053
BsiSI CCGG 6 cut(s) 254, 305, 351, 615, 837, 887
BslFI GGGAC 2 cut(s) 404, 643
BslI CCNNNNNNNGG 7 cut(s) 50, 253, 650, 651, 777, 790, 826
BsmAI GTCTC 2 cut(s) 127, 455
BsmFI GGGAC 2 cut(s) 404, 643
BsnI GGCC 7 cut(s) 43, 54, 134, 170, 224, 794, 1181
Bso31I GGTCTC 1 cut(s) 127
BsoBI CYCGRG 2 cut(s) 475, 1053
Bsp1286I GDGCHC 7 cut(s) 337, 360, 490, 744, 933, 1054, 1060
Bsp13I TCCGGA 1 cut(s) 304
Bsp1407I TGTACA 2 cut(s) 660, 963
Bsp1720I GCTNAGC 1 cut(s) 948
Bsp19I CCATGG 1 cut(s) 429
BspANI GGCC 7 cut(s) 43, 54, 134, 170, 224, 794, 1181
BspCNI CTCAG 3 cut(s) 394, 505, 961
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 5 cut(s) 410, 482, 746, 748, 983
BspLI GGNNCC 6 cut(s) 613, 760, 782, 831, 1083, 1137
BspPI GGATC 7 cut(s) 267, 330, 540, 728, 824, 837, 962
BspT107I GGYRCC 3 cut(s) 758, 780, 1135
BspTNI GGTCTC 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 762, 849
BsrFI RCCGGY 4 cut(s) 253, 350, 614, 886
BsrGI TGTACA 2 cut(s) 660, 963
BsrI ACTGG 2 cut(s) 469, 737
BssAI RCCGGY 4 cut(s) 253, 350, 614, 886
BssECI CCNNGG 4 cut(s) 117, 129, 429, 626
BssHII GCGCGC 1 cut(s) 746
BssNI GRCGYC 2 cut(s) 781, 1136
BssSI CACGAG 2 cut(s) 383, 785
BssT1I CCWWGG 1 cut(s) 429
Bst2BI CACGAG 2 cut(s) 383, 785
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 3 cut(s) 121, 595, 1011
Bst6I CTCTTC 1 cut(s) 306
BstACI GRCGYC 2 cut(s) 781, 1136
BstAUI TGTACA 2 cut(s) 660, 963
BstDEI CTNAG 3 cut(s) 402, 513, 948
BstDSI CCRYGG 3 cut(s) 117, 129, 429
BstF5I GGATG 3 cut(s) 463, 767, 1120
BstFNI CGCG 5 cut(s) 410, 482, 746, 748, 983
BstH2I RGCGCY 3 cut(s) 383, 784, 1139
BstHHI GCGC 7 cut(s) 382, 482, 748, 750, 783, 892, 1138
BstMAI GTCTC 2 cut(s) 127, 455
BstMCI CGRYCG 1 cut(s) 855
BstMWI GCNNNNNNNGC 7 cut(s) 10, 716, 756, 848, 944, 1049, 1142
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 1 cut(s) 625
BstUI CGCG 5 cut(s) 410, 482, 746, 748, 983
BstV1I GCAGC 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 918
BstX2I RGATCY 2 cut(s) 532, 829
BstYI RGATCY 2 cut(s) 532, 829
BsuRI GGCC 7 cut(s) 43, 54, 134, 170, 224, 794, 1181
BtgI CCRYGG 3 cut(s) 117, 129, 429
BtrI CACGTC 2 cut(s) 235, 666
BtsCI GGATG 3 cut(s) 463, 767, 1120
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 3 cut(s) 176, 269, 744
CfoI GCGC 7 cut(s) 382, 482, 748, 750, 783, 892, 1138
Cfr10I RCCGGY 4 cut(s) 253, 350, 614, 886
Cfr13I GGNCC 2 cut(s) 169, 222
CseI GACGC 1 cut(s) 416
Csp6I GTAC 5 cut(s) 370, 596, 661, 901, 964
CspAI ACCGGT 1 cut(s) 253
CviAII CATG 6 cut(s) 39, 394, 430, 527, 619, 862
CviQI GTAC 5 cut(s) 370, 596, 661, 901, 964
DdeI CTNAG 3 cut(s) 402, 513, 948
DinI GGCGCC 2 cut(s) 782, 1137
EaeI YGGCCR 2 cut(s) 52, 132
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
EciI GGCGGA 4 cut(s) 115, 986, 1058, 1127
Ecl136II GAGCTC 1 cut(s) 358
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 3 cut(s) 337, 360, 490
Eco31I GGTCTC 1 cut(s) 127
Eco32I GATATC 1 cut(s) 988
Eco53kI GAGCTC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 1085
Eco88I CYCGRG 2 cut(s) 475, 1053
EcoICRI GAGCTC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 625
EcoRV GATATC 1 cut(s) 988
EcoT14I CCWWGG 1 cut(s) 429
EcoT38I GRGCYC 3 cut(s) 337, 360, 490
EgeI GGCGCC 2 cut(s) 782, 1137
EheI GGCGCC 2 cut(s) 782, 1137
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 6 cut(s) 42, 397, 433, 530, 622, 865
FaiI YATR 9 cut(s) 40, 395, 431, 528, 547, 575, 620, 863, 899
FaqI GGGAC 2 cut(s) 404, 643
FatI CATG 6 cut(s) 38, 393, 429, 526, 618, 861
FauI CCCGC 1 cut(s) 153
Fnu4HI GCNGC 7 cut(s) 182, 272, 730, 757, 939, 1041, 1139
FokI GGATG 3 cut(s) 470, 754, 1127
FriOI GRGCYC 3 cut(s) 337, 360, 490
Fsp4HI GCNGC 7 cut(s) 182, 272, 730, 757, 939, 1041, 1139
FspBI CTAG 5 cut(s) 212, 276, 443, 471, 711
GlaI GCGC 7 cut(s) 381, 481, 747, 749, 782, 891, 1137
GluI GCNGC 7 cut(s) 182, 272, 730, 757, 939, 1041, 1139
GsaI CCCAGC 1 cut(s) 223
GsuI CTGGAG 1 cut(s) 1049
HaeII RGCGCY 3 cut(s) 383, 784, 1139
HaeIII GGCC 7 cut(s) 43, 54, 134, 170, 224, 794, 1181
HapII CCGG 6 cut(s) 254, 305, 351, 615, 837, 887
HgaI GACGC 1 cut(s) 416
HhaI GCGC 7 cut(s) 382, 482, 748, 750, 783, 892, 1138
Hin1I GRCGYC 2 cut(s) 781, 1136
Hin1II CATG 6 cut(s) 42, 397, 433, 530, 622, 865
Hin6I GCGC 7 cut(s) 380, 480, 746, 748, 781, 890, 1136
HinP1I GCGC 7 cut(s) 380, 480, 746, 748, 781, 890, 1136
HinfI GANTC 3 cut(s) 293, 496, 622
HpaII CCGG 6 cut(s) 254, 305, 351, 615, 837, 887
Hpy166II GTNNAC 4 cut(s) 661, 663, 964, 966
Hpy188I TCNGA 4 cut(s) 107, 772, 1007, 1086
Hpy188III TCNNGA 5 cut(s) 305, 320, 580, 880, 1103
Hpy8I GTNNAC 4 cut(s) 661, 663, 964, 966
Hpy99I CGWCG 1 cut(s) 776
HpyAV CCTTC 6 cut(s) 54, 160, 292, 448, 1040, 1089
HpyCH4III ACNGT 3 cut(s) 121, 595, 1011
HpyCH4IV ACGT 3 cut(s) 234, 665, 686
HpyCH4V TGCA 3 cut(s) 4, 38, 767
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 716, 756, 848, 944, 1049, 1142
HpyF3I CTNAG 3 cut(s) 402, 513, 948
HpySE526I ACGT 3 cut(s) 234, 665, 686
Hsp92I GRCGYC 2 cut(s) 781, 1136
Hsp92II CATG 6 cut(s) 42, 397, 433, 530, 622, 865
HspAI GCGC 7 cut(s) 380, 480, 746, 748, 781, 890, 1136
KasI GGCGCC 2 cut(s) 780, 1135
Kpn2I TCCGGA 1 cut(s) 304
KroI GCCGGC 2 cut(s) 614, 886
KroNI GCCGGC 2 cut(s) 616, 888
LmnI GCTCC 6 cut(s) 363, 543, 611, 822, 839, 1087
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 3 cut(s) 776, 855, 873
MaeI CTAG 5 cut(s) 212, 276, 443, 471, 711
MaeII ACGT 3 cut(s) 234, 665, 686
MboII GAAGA 7 cut(s) 320, 323, 883, 886, 923, 931, 1141
MflI RGATCY 2 cut(s) 532, 829
MhlI GDGCHC 7 cut(s) 337, 360, 490, 744, 933, 1054, 1060
MlsI TGGCCA 2 cut(s) 54, 134
MluCI AATT 3 cut(s) 101, 1015, 1089
MluI ACGCGT 1 cut(s) 408
MluNI TGGCCA 2 cut(s) 54, 134
Mly113I GGCGCC 2 cut(s) 781, 1136
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 795
Mox20I TGGCCA 2 cut(s) 54, 134
MroI TCCGGA 1 cut(s) 304
MroNI GCCGGC 2 cut(s) 614, 886
MroXI GAANNNNTTC 2 cut(s) 315, 923
MscI TGGCCA 2 cut(s) 54, 134
MseI TTAA 2 cut(s) 1149, 1167
Msp20I TGGCCA 2 cut(s) 54, 134
MspA1I CMGCKG 1 cut(s) 952
MspI CCGG 6 cut(s) 254, 305, 351, 615, 837, 887
MspR9I CCNGG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 627
MvnI CGCG 5 cut(s) 410, 482, 746, 748, 983
MwoI GCNNNNNNNGC 7 cut(s) 10, 716, 756, 848, 944, 1049, 1142
NaeI GCCGGC 2 cut(s) 616, 888
NarI GGCGCC 2 cut(s) 781, 1136
NcoI CCATGG 1 cut(s) 429
NgoMIV GCCGGC 2 cut(s) 614, 886
NlaIII CATG 6 cut(s) 42, 397, 433, 530, 622, 865
NlaIV GGNNCC 6 cut(s) 613, 760, 782, 831, 1083, 1137
NmeAIII GCCGAG 2 cut(s) 139, 250
PaeR7I CTCGAG 1 cut(s) 1053
PauI GCGCGC 1 cut(s) 746
PcsI WCGNNNNNNNCGW 1 cut(s) 591
PdiI GCCGGC 2 cut(s) 616, 888
PdmI GAANNNNTTC 2 cut(s) 315, 923
PfeI GAWTC 2 cut(s) 496, 622
PflMI CCANNNNNTGG 1 cut(s) 790
PinAI ACCGGT 1 cut(s) 253
PkrI GCNGC 7 cut(s) 183, 273, 731, 758, 940, 1042, 1140
Ple19I CGATCG 1 cut(s) 855
PleI GAGTC 1 cut(s) 287
PluTI GGCGCC 2 cut(s) 784, 1139
PpsI GAGTC 1 cut(s) 287
Psp124BI GAGCTC 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 625
PspFI CCCAGC 1 cut(s) 219
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 6 cut(s) 613, 760, 782, 831, 1083, 1137
PspPI GGNCC 2 cut(s) 169, 222
PspXI VCTCGAGB 1 cut(s) 1053
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PsuI RGATCY 2 cut(s) 532, 829
PteI GCGCGC 1 cut(s) 746
PvuI CGATCG 1 cut(s) 855
RsaI GTAC 5 cut(s) 371, 597, 662, 902, 965
RsaNI GTAC 5 cut(s) 370, 596, 661, 901, 964
SacI GAGCTC 1 cut(s) 360
SaqAI TTAA 2 cut(s) 1149, 1167
SatI GCNGC 7 cut(s) 182, 272, 730, 757, 939, 1041, 1139
Sau96I GGNCC 2 cut(s) 169, 222
ScaI AGTACT 1 cut(s) 371
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 627
SduI GDGCHC 7 cut(s) 337, 360, 490, 744, 933, 1054, 1060
SfaNI GCATC 3 cut(s) 776, 855, 873
SfoI GGCGCC 2 cut(s) 782, 1137
Sfr274I CTCGAG 1 cut(s) 1053
SlaI CTCGAG 1 cut(s) 1053
SmlI CTYRAG 2 cut(s) 578, 1053
SmoI CTYRAG 2 cut(s) 578, 1053
Sse9I AATT 3 cut(s) 101, 1015, 1089
SspDI GGCGCC 2 cut(s) 780, 1135
SspMI CTAG 5 cut(s) 212, 276, 443, 471, 711
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 625
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 3 cut(s) 121, 595, 1011
TaiI ACGT 3 cut(s) 237, 668, 689
TaqI TCGA 7 cut(s) 346, 490, 570, 585, 822, 927, 1054
TaqII GACCGA 1 cut(s) 489
TasI AATT 3 cut(s) 101, 1015, 1089
TatI WGTACW 3 cut(s) 369, 660, 963
TauI GCSGC 6 cut(s) 184, 732, 759, 941, 1043, 1141
TfiI GAWTC 2 cut(s) 496, 622
Tru1I TTAA 2 cut(s) 1149, 1167
Tru9I TTAA 2 cut(s) 1149, 1167
TscAI CASTG 3 cut(s) 183, 269, 744
TseI GCWGC 1 cut(s) 271
TspDTI ATGAA 1 cut(s) 635
TspGWI ACGGA 2 cut(s) 329, 1141
TspRI CASTG 3 cut(s) 183, 269, 744
Van91I CCANNNNNTGG 1 cut(s) 790
XapI RAATTY 2 cut(s) 101, 1015
XcmI CCANNNNNNNNNTGG 1 cut(s) 841
XhoI CTCGAG 1 cut(s) 1053
XmnI GAANNNNTTC 2 cut(s) 315, 923
XspI CTAG 5 cut(s) 212, 276, 443, 471, 711
ZrmI AGTACT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.