Rw2G024760

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007963
Physical Location & Seq
Forward (+)
36754895 .. 36757619
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G024760.1

Sequence Viewer

Length: 1182 bp
ATGCAAGCCTCCCTCTACTTGGACACCAAAACTCTCACATGGCCTTCACGTCGCCACACCTCAACCCACCACATATTTCGGCCAACACCCACAGTCAGATTCTGGCCATTGCCTCTAGTCTCCGCCGTGGCCACGTCACCTCCACCTCTAACTCGGCAGAAGACCCACTCGTTGCCACCGGAAAAGGTGGAGCTCTTAAAGTCACTAGAGGGCTGGGCCTCCCAGACAATCCTCCCATTTTTGAAGCCAGTCGATGAGTGCTGGCAACCCAGTACCTTCTTGCCAGACCCATCGCTCCCCAAAGAGGAGTTCTTGGACCGTGTACGGGCCCTTAGGGACCGGACAGATGGGCTACCTGACGAGTACTTTGTGGTGCTGGTCGGGGATATGATCACCGAGGAGGTGCTGCCTGCGTACCAGAGTATGCTCAATGGGCTCGACGGGGTGAGAGACGAGTCTGGAGTTAGCTCGAGCCCGTGGGCGCAATGGATTCGAGCTTGGTCTGCTGAGGAAAACCGCCACGGCGATCTGCTCAAAACCTTTCTGTACCTGTCCGGTCGGGTTGATATGTTCAAGATCGAGAAGACTATTCAGAACCTTATTGGAGCTGGCATGGAGCCAGGAACGGAGAACAACCCGTACTTGGGTTTCGTGTACGCGTCATTTCAAGAGCGAGCCACGTTCATATCTCACGGCAACACGGCTCGGCTGGCTAAGGACTGTGGTGATCCGGTGCTTGCCCGCATATGCGGCACCATTGCATCCGATGAGAGGCGCCACGAGTACGCCTATTCCAAGATTGTAGAAAAGCTACTTGAAGTGGACCCGACTACAGCAATGGTTGCCATTGCAGACATGATGAGGAAGAAGATCATAATGCCGGGTCATCTCATGTATGATGGGGAGGACCCCAAGCTATACGAGCACTTTGGGGCCGTGGCTCAACGGCTTGGGGTGTACACAACACATGATTATGCGGATATTTTGGAGTTTTTTATCGGACGGTGGAGGCTGGAGAAGATGGAGGGATTGACGGGAGAGGGTAAGCGTGCACAAGACTTTGTGTGTGGGTTGTTGGTGAGGATCAGGAGGCTAGAAGAGTGGGCCGATGAGCGAGCAAGAAAGATGAAGCCTCATGGCGTCAAGTTTAGCTGGATTTATAATAAAGAAGTGGTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

393

Amino Acids

44.91

Weight (kDa)

6.29

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 61 - 386 1.6e-146 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AccB1I GGYRCC 2 cut(s) 752, 774
AccII CGCG 1 cut(s) 659
AciI CCGC 5 cut(s) 123, 517, 742, 750, 977
AclWI GGATC 2 cut(s) 722, 1091
AcoI YGGCCR 3 cut(s) 80, 104, 129
AcyI GRCGYC 2 cut(s) 775, 1140
AfaI GTAC 9 cut(s) 274, 324, 365, 416, 548, 641, 656, 785, 959
AfiI CCNNNNNNNGG 6 cut(s) 19, 304, 325, 643, 644, 771
AflIII ACRYGT 1 cut(s) 657
AgsI TTSAA 4 cut(s) 244, 574, 668, 818
AjiI CACGTC 2 cut(s) 50, 135
AjnI CCWGG 1 cut(s) 619
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 7 cut(s) 193, 468, 497, 608, 811, 916, 1152
AluI AGCT 7 cut(s) 193, 468, 497, 608, 811, 916, 1152
Alw21I GWGCWC 3 cut(s) 195, 927, 1054
Alw26I GTCTC 2 cut(s) 124, 444
Alw44I GTGCAC 1 cut(s) 1050
AlwI GGATC 2 cut(s) 722, 1091
AlwNI CAGNNNCTG 1 cut(s) 102
Ama87I CYCGRG 1 cut(s) 469
AoxI GGCC 8 cut(s) 41, 80, 104, 129, 216, 327, 933, 1104
ApaI GGGCCC 1 cut(s) 331
ApaLI GTGCAC 1 cut(s) 1050
ApeKI GCWGC 1 cut(s) 406
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 2 cut(s) 484, 777
AspS9I GGNCC 9 cut(s) 216, 316, 327, 328, 337, 823, 907, 933, 1104
AsuC2I CCSGG 1 cut(s) 882
AsuHPI GGTGA 5 cut(s) 129, 385, 457, 737, 1090
AvaI CYCGRG 1 cut(s) 469
AvaII GGWCC 4 cut(s) 316, 337, 823, 907
AxyI CCTNAGG 1 cut(s) 332
BaeGI GKGCMC 2 cut(s) 331, 1054
BalI TGGCCA 2 cut(s) 106, 131
BanI GGYRCC 2 cut(s) 752, 774
BanII GRGCYC 4 cut(s) 195, 331, 438, 476
BauI CACGAG 1 cut(s) 779
BbsI GAAGAC 2 cut(s) 167, 590
Bbv12I GWGCWC 3 cut(s) 195, 927, 1054
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 393
BccI CCATC 4 cut(s) 298, 341, 893, 1015
BceAI ACGGC 6 cut(s) 110, 538, 709, 717, 920, 962
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 1 cut(s) 621
BclI TGATCA 1 cut(s) 390
BcnI CCSGG 1 cut(s) 882
BcoDI GTCTC 2 cut(s) 124, 444
BfaI CTAG 3 cut(s) 116, 206, 1094
BfmI CTRYAG 1 cut(s) 831
BfoI RGCGCY 1 cut(s) 778
BisI GCNGC 2 cut(s) 407, 751
BlsI GCNGC 2 cut(s) 408, 752
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 2 cut(s) 621, 882
Bme18I GGWCC 4 cut(s) 316, 337, 823, 907
BmeT110I CYCGRG 1 cut(s) 469
BmgBI CACGTC 2 cut(s) 50, 135
BmgT120I GGNCC 9 cut(s) 216, 316, 327, 328, 337, 823, 907, 933, 1104
BmiI GGNNCC 8 cut(s) 329, 338, 618, 754, 776, 825, 909, 934
BmrFI CCNGG 2 cut(s) 621, 882
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 770
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 167, 590
BpmI CTGGAG 2 cut(s) 480, 1034
Bpu10I CCTNAGC 2 cut(s) 507, 714
BpuMI CCSGG 1 cut(s) 882
BsaHI GRCGYC 2 cut(s) 775, 1140
BsaJI CCNNGG 5 cut(s) 126, 396, 476, 520, 936
BsaWI WCCGGW 4 cut(s) 178, 339, 554, 730
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 304, 325, 643, 644, 771
Bse1I ACTGG 2 cut(s) 248, 270
Bse21I CCTNAGG 1 cut(s) 332
Bse3DI GCAATG 5 cut(s) 107, 491, 756, 843, 846
BseBI CCWGG 1 cut(s) 621
BseDI CCNNGG 5 cut(s) 126, 396, 476, 520, 936
BseGI GGATG 1 cut(s) 761
BseLI CCNNNNNNNGG 6 cut(s) 19, 304, 325, 643, 644, 771
BseMI GCAATG 5 cut(s) 107, 491, 756, 843, 846
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 2 cut(s) 248, 270
BseRI GAGGAG 2 cut(s) 320, 413
BseSI GKGCMC 2 cut(s) 331, 1054
BseXI GCAGC 1 cut(s) 393
BseYI CCCAGC 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 659
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 8 cut(s) 43, 82, 106, 131, 218, 329, 935, 1106
BshNI GGYRCC 2 cut(s) 752, 774
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 3 cut(s) 195, 927, 1054
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 5 cut(s) 179, 340, 555, 731, 881
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 6 cut(s) 19, 304, 325, 643, 644, 771
BsmAI GTCTC 2 cut(s) 124, 444
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 8 cut(s) 43, 82, 106, 131, 218, 329, 935, 1106
BsoBI CYCGRG 1 cut(s) 469
Bsp120I GGGCCC 1 cut(s) 327
Bsp1286I GDGCHC 6 cut(s) 195, 331, 438, 476, 927, 1054
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 6 cut(s) 390, 526, 576, 727, 870, 1083
BspACI CCGC 5 cut(s) 123, 517, 742, 750, 977
BspANI GGCC 8 cut(s) 43, 82, 106, 131, 218, 329, 935, 1106
BspCNI CTCAG 1 cut(s) 499
BspFNI CGCG 1 cut(s) 659
BspLI GGNNCC 8 cut(s) 329, 338, 618, 754, 776, 825, 909, 934
BspPI GGATC 2 cut(s) 722, 1091
BspT107I GGYRCC 2 cut(s) 752, 774
BsrDI GCAATG 5 cut(s) 107, 491, 756, 843, 846
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 2 cut(s) 248, 270
BssECI CCNNGG 5 cut(s) 126, 396, 476, 520, 936
BssMI GATC 6 cut(s) 390, 526, 576, 727, 870, 1083
BssNI GRCGYC 2 cut(s) 775, 1140
BssSI CACGAG 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 1 cut(s) 621
Bst4CI ACNGT 4 cut(s) 94, 320, 722, 1005
Bst6I CTCTTC 1 cut(s) 1092
BstACI GRCGYC 2 cut(s) 775, 1140
BstAPI GCANNNNNTGC 1 cut(s) 842
BstAUI TGTACA 1 cut(s) 957
BstDEI CTNAG 3 cut(s) 332, 507, 714
BstDSI CCRYGG 4 cut(s) 126, 476, 520, 936
BstF5I GGATG 1 cut(s) 761
BstFNI CGCG 1 cut(s) 659
BstH2I RGCGCY 1 cut(s) 778
BstHHI GCGC 2 cut(s) 484, 777
BstKTI GATC 6 cut(s) 393, 529, 579, 730, 873, 1086
BstMAI GTCTC 2 cut(s) 124, 444
BstMBI GATC 6 cut(s) 390, 526, 576, 727, 870, 1083
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 6 cut(s) 433, 503, 710, 750, 842, 922
BstNI CCWGG 1 cut(s) 621
BstSCI CCNGG 2 cut(s) 619, 880
BstSFI CTRYAG 1 cut(s) 831
BstSLI GKGCMC 2 cut(s) 331, 1054
BstUI CGCG 1 cut(s) 659
BstV1I GCAGC 1 cut(s) 393
BstV2I GAAGAC 2 cut(s) 167, 590
Bsu36I CCTNAGG 1 cut(s) 332
BsuRI GGCC 8 cut(s) 43, 82, 106, 131, 218, 329, 935, 1106
BtgI CCRYGG 4 cut(s) 126, 476, 520, 936
BtgZI GCGATG 1 cut(s) 276
BtrI CACGTC 2 cut(s) 50, 135
BtsCI GGATG 1 cut(s) 761
CaiI CAGNNNCTG 1 cut(s) 102
CfoI GCGC 2 cut(s) 484, 777
Cfr13I GGNCC 9 cut(s) 216, 316, 327, 328, 337, 823, 907, 933, 1104
CseI GACGC 2 cut(s) 648, 1129
Csp6I GTAC 9 cut(s) 273, 323, 364, 415, 547, 640, 655, 784, 958
CviAII CATG 6 cut(s) 39, 613, 856, 892, 968, 1136
CviQI GTAC 9 cut(s) 273, 323, 364, 415, 547, 640, 655, 784, 958
DdeI CTNAG 3 cut(s) 332, 507, 714
DinI GGCGCC 1 cut(s) 776
DpnI GATC 6 cut(s) 392, 528, 578, 729, 872, 1085
DpnII GATC 6 cut(s) 390, 526, 576, 727, 870, 1083
EaeI YGGCCR 3 cut(s) 80, 104, 129
Eam1104I CTCTTC 1 cut(s) 1092
EarI CTCTTC 1 cut(s) 1092
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 4 cut(s) 195, 331, 438, 476
Eco47I GGWCC 4 cut(s) 316, 337, 823, 907
Eco53kI GAGCTC 1 cut(s) 193
Eco81I CCTNAGG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 2 cut(s) 328, 907
EcoRII CCWGG 1 cut(s) 619
EcoT38I GRGCYC 4 cut(s) 195, 331, 438, 476
EgeI GGCGCC 1 cut(s) 776
EheI GGCGCC 1 cut(s) 776
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 6 cut(s) 42, 616, 859, 895, 971, 1139
FaqI GGGAC 1 cut(s) 350
FatI CATG 6 cut(s) 38, 612, 855, 891, 967, 1135
FauI CCCGC 1 cut(s) 749
FauNDI CATATG 1 cut(s) 746
FbaI TGATCA 1 cut(s) 390
Fnu4HI GCNGC 2 cut(s) 407, 751
FokI GGATG 1 cut(s) 748
FriOI GRGCYC 4 cut(s) 195, 331, 438, 476
Fsp4HI GCNGC 2 cut(s) 407, 751
FspBI CTAG 3 cut(s) 116, 206, 1094
GlaI GCGC 2 cut(s) 483, 776
GluI GCNGC 2 cut(s) 407, 751
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 2 cut(s) 480, 1034
HaeII RGCGCY 1 cut(s) 778
HaeIII GGCC 8 cut(s) 43, 82, 106, 131, 218, 329, 935, 1106
HapII CCGG 5 cut(s) 179, 340, 555, 731, 881
HgaI GACGC 2 cut(s) 648, 1129
HhaI GCGC 2 cut(s) 484, 777
Hin1I GRCGYC 2 cut(s) 775, 1140
Hin1II CATG 6 cut(s) 42, 616, 859, 895, 971, 1139
Hin6I GCGC 2 cut(s) 482, 775
HinP1I GCGC 2 cut(s) 482, 775
HinfI GANTC 3 cut(s) 99, 455, 490
HpaII CCGG 5 cut(s) 179, 340, 555, 731, 881
HphI GGTGA 5 cut(s) 129, 385, 457, 737, 1090
Hpy166II GTNNAC 6 cut(s) 323, 655, 823, 958, 960, 1052
Hpy188I TCNGA 4 cut(s) 98, 594, 766, 1001
Hpy188III TCNNGA 5 cut(s) 459, 574, 580, 668, 1087
Hpy8I GTNNAC 6 cut(s) 323, 655, 823, 958, 960, 1052
Hpy99I CGWCG 2 cut(s) 54, 443
HpyAV CCTTC 2 cut(s) 54, 286
HpyCH4III ACNGT 4 cut(s) 94, 320, 722, 1005
HpyCH4IV ACGT 3 cut(s) 49, 134, 680
HpyCH4V TGCA 4 cut(s) 4, 761, 851, 1052
HpyF10VI GCNNNNNNNGC 6 cut(s) 433, 503, 710, 750, 842, 922
HpyF3I CTNAG 3 cut(s) 332, 507, 714
HpySE526I ACGT 3 cut(s) 49, 134, 680
Hsp92I GRCGYC 2 cut(s) 775, 1140
Hsp92II CATG 6 cut(s) 42, 616, 859, 895, 971, 1139
HspAI GCGC 2 cut(s) 482, 775
KasI GGCGCC 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 6 cut(s) 390, 526, 576, 727, 870, 1083
LmnI GCTCC 4 cut(s) 190, 300, 605, 616
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 1 cut(s) 770
MaeI CTAG 3 cut(s) 116, 206, 1094
MaeII ACGT 3 cut(s) 49, 134, 680
MaeIII GTNAC 2 cut(s) 135, 201
MalI GATC 6 cut(s) 392, 528, 578, 729, 872, 1085
MboI GATC 6 cut(s) 390, 526, 576, 727, 870, 1083
MboII GAAGA 6 cut(s) 172, 595, 877, 880, 1030, 1109
MhlI GDGCHC 6 cut(s) 195, 331, 438, 476, 927, 1054
MlsI TGGCCA 2 cut(s) 106, 131
MluI ACGCGT 1 cut(s) 657
MluNI TGGCCA 2 cut(s) 106, 131
Mly113I GGCGCC 1 cut(s) 775
MlyI GAGTC 1 cut(s) 464
Mox20I TGGCCA 2 cut(s) 106, 131
MscI TGGCCA 2 cut(s) 106, 131
MseI TTAA 1 cut(s) 197
MslI CAYNNNNRTG 1 cut(s) 972
Msp20I TGGCCA 2 cut(s) 106, 131
MspI CCGG 5 cut(s) 179, 340, 555, 731, 881
MspR9I CCNGG 2 cut(s) 621, 882
MvaI CCWGG 1 cut(s) 621
MvnI CGCG 1 cut(s) 659
MwoI GCNNNNNNNGC 6 cut(s) 433, 503, 710, 750, 842, 922
NarI GGCGCC 1 cut(s) 775
NciI CCSGG 1 cut(s) 882
NdeI CATATG 1 cut(s) 746
NdeII GATC 6 cut(s) 390, 526, 576, 727, 870, 1083
NlaIII CATG 6 cut(s) 42, 616, 859, 895, 971, 1139
NlaIV GGNNCC 8 cut(s) 329, 338, 618, 754, 776, 825, 909, 934
NmeAIII GCCGAG 2 cut(s) 133, 685
NmuCI GTSAC 2 cut(s) 135, 201
PaeR7I CTCGAG 1 cut(s) 469
PfeI GAWTC 2 cut(s) 99, 490
PkrI GCNGC 2 cut(s) 408, 752
PleI GAGTC 1 cut(s) 463
PluTI GGCGCC 1 cut(s) 778
PpsI GAGTC 1 cut(s) 463
PpuMI RGGWCCY 1 cut(s) 907
PsiI TTATAA 1 cut(s) 1161
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 1 cut(s) 619
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 1 cut(s) 619
PspN4I GGNNCC 8 cut(s) 329, 338, 618, 754, 776, 825, 909, 934
PspOMI GGGCCC 1 cut(s) 327
PspPI GGNCC 9 cut(s) 216, 316, 327, 328, 337, 823, 907, 933, 1104
PspPPI RGGWCCY 1 cut(s) 907
PspXI VCTCGAGB 1 cut(s) 469
PsrI GAACNNNNNNTAC 2 cut(s) 623, 655
PstNI CAGNNNCTG 1 cut(s) 102
RsaI GTAC 9 cut(s) 274, 324, 365, 416, 548, 641, 656, 785, 959
RsaNI GTAC 9 cut(s) 273, 323, 364, 415, 547, 640, 655, 784, 958
RseI CAYNNNNRTG 1 cut(s) 972
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 2 cut(s) 407, 751
Sau3AI GATC 6 cut(s) 390, 526, 576, 727, 870, 1083
Sau96I GGNCC 9 cut(s) 216, 316, 327, 328, 337, 823, 907, 933, 1104
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 2 cut(s) 621, 882
SduI GDGCHC 6 cut(s) 195, 331, 438, 476, 927, 1054
SfaNI GCATC 1 cut(s) 770
SfcI CTRYAG 1 cut(s) 831
SfoI GGCGCC 1 cut(s) 776
Sfr274I CTCGAG 1 cut(s) 469
SinI GGWCC 4 cut(s) 316, 337, 823, 907
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 972
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
SsiI CCGC 5 cut(s) 123, 517, 742, 750, 977
SspDI GGCGCC 1 cut(s) 774
SspMI CTAG 3 cut(s) 116, 206, 1094
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 619, 880
TaaI ACNGT 4 cut(s) 94, 320, 722, 1005
TaiI ACGT 3 cut(s) 52, 137, 683
TaqI TCGA 5 cut(s) 252, 438, 470, 493, 579
TatI WGTACW 2 cut(s) 363, 957
TauI GCSGC 1 cut(s) 753
TfiI GAWTC 2 cut(s) 99, 490
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TseFI GTSAC 2 cut(s) 135, 201
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 2 cut(s) 135, 201
TspDTI ATGAA 2 cut(s) 673, 1142
TspGWI ACGGA 1 cut(s) 641
VneI GTGCAC 1 cut(s) 1050
VpaK11BI GGWCC 4 cut(s) 316, 337, 823, 907
XhoI CTCGAG 1 cut(s) 469
XspI CTAG 3 cut(s) 116, 206, 1094
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.