Rh2DG331000

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007963
Physical Location & Seq
Forward (+)
42249260 .. 42251785
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG331000.1

Sequence Viewer

Length: 1182 bp
ATGCAAGCCTCCCTCTACCTGGACACCAAAACTCTCGCATGGCCTTCACGTGACCACACCTCAACCCACCACATATTTCGGCCAACACCCACAGTCAGATTCAGGCCATTGCCTCTAGTCTCCGCCGTGGCCACGTCACCTCCACCTCTAACTCGACAGAAGACCCCCTCGTTGCCACCGGAAAAGGTGGAGATCTTAAAGTCACTAGAGGGCTGGGCCTCCCAGACAATCCTTCCATTTTTGAAGCCGGTCGATGAGTGCTGGCAACCCAGTACCTTCCTGCCAGACCCATCGCTCCCCAAAGAGGAGTTCTTGGACCGTGTACGGGCACTTAGGGACCGGACAGATGGGCTGCCTGACGAGTACTTTGTGGTGCTGATCGGGGATATGATCTCCGAAGAGGTGTTGCCTGCGTACCAGAGTATGCTCAATGGGCTCGACGGAGTGAGAGACGAGTCCGGAGTTAGCTCGAGCCCGTGGGCGCAGTGGATTCGGGCTTGGTCCGCTGAGGAAAACCGCCACGGCGATCTGCTCAAAACCTTTCTGTACCTGTCCGGTCGGGTTGATATGTTCAAGATCGAGAAGACTATTCAGAACCTTATTGGAGCTGGCATGGAGCCAGGAACGGAGAACAACCCGTACTTGGGTTTCGTGTACGCATCATTTCAAGAGCGAGCCACGTTCATATCGCACGGCAACACGGCTCGGCTGGCTAAGGACTGTGGTGATCCGGTGCTTGCCCGCATATGCGGCACCATTGCATCCGATGAGAGGCGCCACGAGTTCGCCTATTCCAAGATTGTAGAAAAGCTACTTGAAGTGGACCCGACTAAAGCAATGGTCGCCATTGCAGACATGATGAGGAAGAAGATCATAATGCCGGGTCATCTCATGTATGATGGGGAGGACCCCAAGCTGTACGAGCACTTTGGGGCCGTGGCTCAACGGCTTGGGGTGTACACAACACATAATTATGCGGATATTTTAGAGTTTTTTATCGGACGGTGGAGGCTGGAGAAGATGGAGGGATTAACGGGAGAGGGTAAGTGTGCACAAGACTTTGTGTGTGGGTTGTTGGTGAGGATCAGGAGGCTAGAAGAGTGGGCTGATGAGCGAGCAAGAAAGATGAAGCCGCATGGCGTCAAGTTTAGCTGGATTTATAATAAAGAAGTGGTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

393

Amino Acids

44.73

Weight (kDa)

6.24

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 61 - 386 5e-144 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AccB1I GGYRCC 2 cut(s) 752, 774
AccIII TCCGGA 1 cut(s) 458
AciI CCGC 7 cut(s) 123, 504, 517, 742, 750, 977, 1133
AclWI GGATC 2 cut(s) 722, 1091
AcoI YGGCCR 2 cut(s) 80, 129
AcvI CACGTG 1 cut(s) 50
AcyI GRCGYC 2 cut(s) 775, 1140
AfaI GTAC 9 cut(s) 274, 324, 365, 416, 548, 641, 656, 920, 959
AfiI CCNNNNNNNGG 6 cut(s) 19, 304, 325, 643, 644, 771
AgsI TTSAA 4 cut(s) 244, 574, 668, 818
AjiI CACGTC 1 cut(s) 135
AjnI CCWGG 2 cut(s) 18, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 5 cut(s) 468, 608, 811, 916, 1152
AluI AGCT 5 cut(s) 468, 608, 811, 916, 1152
Alw21I GWGCWC 2 cut(s) 927, 1054
Alw26I GTCTC 2 cut(s) 124, 444
Alw44I GTGCAC 1 cut(s) 1050
AlwI GGATC 2 cut(s) 722, 1091
Ama87I CYCGRG 1 cut(s) 469
Aor13HI TCCGGA 1 cut(s) 458
AoxI GGCC 6 cut(s) 41, 80, 104, 129, 216, 933
ApaLI GTGCAC 1 cut(s) 1050
ApeKI GCWGC 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 2 cut(s) 484, 777
AspS9I GGNCC 7 cut(s) 216, 316, 337, 501, 823, 907, 933
AsuC2I CCSGG 1 cut(s) 882
AsuHPI GGTGA 3 cut(s) 129, 737, 1090
AvaI CYCGRG 1 cut(s) 469
AvaII GGWCC 5 cut(s) 316, 337, 501, 823, 907
BaeGI GKGCMC 2 cut(s) 331, 1054
BalI TGGCCA 1 cut(s) 131
BanI GGYRCC 2 cut(s) 752, 774
BanII GRGCYC 2 cut(s) 438, 476
BauI CACGAG 1 cut(s) 779
BbrPI CACGTG 1 cut(s) 50
BbsI GAAGAC 2 cut(s) 167, 590
Bbv12I GWGCWC 2 cut(s) 927, 1054
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 339
BccI CCATC 4 cut(s) 298, 341, 893, 1015
BceAI ACGGC 6 cut(s) 110, 538, 709, 717, 920, 962
BcgI CGANNNNNNTGC 2 cut(s) 473, 507
BciT130I CCWGG 2 cut(s) 20, 621
BcnI CCSGG 1 cut(s) 882
BcoDI GTCTC 2 cut(s) 124, 444
BfaI CTAG 3 cut(s) 116, 206, 1094
BfoI RGCGCY 1 cut(s) 778
BglII AGATCT 1 cut(s) 192
BisI GCNGC 3 cut(s) 353, 751, 1133
BlsI GCNGC 3 cut(s) 354, 752, 1134
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 3 cut(s) 20, 621, 882
Bme18I GGWCC 5 cut(s) 316, 337, 501, 823, 907
BmeT110I CYCGRG 1 cut(s) 469
BmgBI CACGTC 1 cut(s) 135
BmgT120I GGNCC 7 cut(s) 216, 316, 337, 501, 823, 907, 933
BmiI GGNNCC 7 cut(s) 338, 618, 754, 776, 825, 909, 934
BmrFI CCNGG 3 cut(s) 20, 621, 882
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 668, 770
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 167, 590
BpmI CTGGAG 1 cut(s) 1034
Bpu10I CCTNAGC 2 cut(s) 507, 714
BpuMI CCSGG 1 cut(s) 882
BsaAI YACGTR 1 cut(s) 50
BsaHI GRCGYC 2 cut(s) 775, 1140
BsaJI CCNNGG 4 cut(s) 126, 476, 520, 936
BsaWI WCCGGW 5 cut(s) 178, 339, 458, 554, 730
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 304, 325, 643, 644, 771
Bse118I RCCGGY 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 270
Bse3DI GCAATG 4 cut(s) 107, 756, 843, 846
BseAI TCCGGA 1 cut(s) 458
BseBI CCWGG 2 cut(s) 20, 621
BseDI CCNNGG 4 cut(s) 126, 476, 520, 936
BseGI GGATG 1 cut(s) 761
BseLI CCNNNNNNNGG 6 cut(s) 19, 304, 325, 643, 644, 771
BseMI GCAATG 4 cut(s) 107, 756, 843, 846
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 320
BseSI GKGCMC 2 cut(s) 331, 1054
BseXI GCAGC 1 cut(s) 339
BseYI CCCAGC 1 cut(s) 213
Bsh1285I CGRYCG 2 cut(s) 252, 559
BshFI GGCC 6 cut(s) 43, 82, 106, 131, 218, 935
BshNI GGYRCC 2 cut(s) 752, 774
BsiEI CGRYCG 2 cut(s) 252, 559
BsiHKAI GWGCWC 2 cut(s) 927, 1054
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 7 cut(s) 179, 248, 340, 459, 555, 731, 881
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 6 cut(s) 19, 304, 325, 643, 644, 771
BsmAI GTCTC 2 cut(s) 124, 444
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 6 cut(s) 43, 82, 106, 131, 218, 935
BsoBI CYCGRG 1 cut(s) 469
Bsp1286I GDGCHC 5 cut(s) 331, 438, 476, 927, 1054
Bsp13I TCCGGA 1 cut(s) 458
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 8 cut(s) 192, 378, 390, 526, 576, 727, 870, 1083
BspACI CCGC 7 cut(s) 123, 504, 517, 742, 750, 977, 1133
BspANI GGCC 6 cut(s) 43, 82, 106, 131, 218, 935
BspCNI CTCAG 1 cut(s) 499
BspEI TCCGGA 1 cut(s) 458
BspLI GGNNCC 7 cut(s) 338, 618, 754, 776, 825, 909, 934
BspPI GGATC 2 cut(s) 722, 1091
BspT107I GGYRCC 2 cut(s) 752, 774
BsrDI GCAATG 4 cut(s) 107, 756, 843, 846
BsrFI RCCGGY 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 1 cut(s) 270
BssAI RCCGGY 1 cut(s) 247
BssECI CCNNGG 4 cut(s) 126, 476, 520, 936
BssMI GATC 8 cut(s) 192, 378, 390, 526, 576, 727, 870, 1083
BssNI GRCGYC 2 cut(s) 775, 1140
BssSI CACGAG 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 2 cut(s) 20, 621
Bst4CI ACNGT 4 cut(s) 94, 320, 722, 1005
Bst6I CTCTTC 2 cut(s) 393, 1092
BstACI GRCGYC 2 cut(s) 775, 1140
BstAUI TGTACA 1 cut(s) 957
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 9 cut(s) 6, 263, 411, 610, 675, 711, 738, 742, 1116
BstDEI CTNAG 3 cut(s) 332, 507, 714
BstDSI CCRYGG 4 cut(s) 126, 476, 520, 936
BstF5I GGATG 1 cut(s) 761
BstH2I RGCGCY 1 cut(s) 778
BstHHI GCGC 2 cut(s) 484, 777
BstKTI GATC 8 cut(s) 195, 381, 393, 529, 579, 730, 873, 1086
BstMAI GTCTC 2 cut(s) 124, 444
BstMBI GATC 8 cut(s) 192, 378, 390, 526, 576, 727, 870, 1083
BstMCI CGRYCG 2 cut(s) 252, 559
BstMWI GCNNNNNNNGC 6 cut(s) 433, 503, 710, 750, 842, 922
BstNI CCWGG 2 cut(s) 20, 621
BstSCI CCNGG 3 cut(s) 18, 619, 880
BstSLI GKGCMC 2 cut(s) 331, 1054
BstV1I GCAGC 1 cut(s) 339
BstV2I GAAGAC 2 cut(s) 167, 590
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 6 cut(s) 43, 82, 106, 131, 218, 935
BtgI CCRYGG 4 cut(s) 126, 476, 520, 936
BtgZI GCGATG 1 cut(s) 276
BtrI CACGTC 1 cut(s) 135
BtsCI GGATG 1 cut(s) 761
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 1 cut(s) 491
Cac8I GCNNGC 9 cut(s) 6, 263, 411, 610, 675, 711, 738, 742, 1116
CfoI GCGC 2 cut(s) 484, 777
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 7 cut(s) 216, 316, 337, 501, 823, 907, 933
CseI GACGC 1 cut(s) 1129
Csp6I GTAC 9 cut(s) 273, 323, 364, 415, 547, 640, 655, 919, 958
CviAII CATG 5 cut(s) 39, 613, 856, 892, 1136
CviQI GTAC 9 cut(s) 273, 323, 364, 415, 547, 640, 655, 919, 958
DdeI CTNAG 3 cut(s) 332, 507, 714
DinI GGCGCC 1 cut(s) 776
DpnI GATC 8 cut(s) 194, 380, 392, 528, 578, 729, 872, 1085
DpnII GATC 8 cut(s) 192, 378, 390, 526, 576, 727, 870, 1083
EaeI YGGCCR 2 cut(s) 80, 129
Eam1104I CTCTTC 2 cut(s) 393, 1092
EarI CTCTTC 2 cut(s) 393, 1092
EciI GGCGGA 1 cut(s) 112
Eco24I GRGCYC 2 cut(s) 438, 476
Eco47I GGWCC 5 cut(s) 316, 337, 501, 823, 907
Eco72I CACGTG 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 2 cut(s) 18, 619
EcoT38I GRGCYC 2 cut(s) 438, 476
EgeI GGCGCC 1 cut(s) 776
EheI GGCGCC 1 cut(s) 776
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 5 cut(s) 42, 616, 859, 895, 1139
FaqI GGGAC 1 cut(s) 350
FatI CATG 5 cut(s) 38, 612, 855, 891, 1135
FauI CCCGC 1 cut(s) 749
FauNDI CATATG 1 cut(s) 746
Fnu4HI GCNGC 3 cut(s) 353, 751, 1133
FokI GGATG 1 cut(s) 748
FriOI GRGCYC 2 cut(s) 438, 476
Fsp4HI GCNGC 3 cut(s) 353, 751, 1133
FspBI CTAG 3 cut(s) 116, 206, 1094
GlaI GCGC 2 cut(s) 483, 776
GluI GCNGC 3 cut(s) 353, 751, 1133
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 1034
HaeII RGCGCY 1 cut(s) 778
HaeIII GGCC 6 cut(s) 43, 82, 106, 131, 218, 935
HapII CCGG 7 cut(s) 179, 248, 340, 459, 555, 731, 881
HgaI GACGC 1 cut(s) 1129
HhaI GCGC 2 cut(s) 484, 777
Hin1I GRCGYC 2 cut(s) 775, 1140
Hin1II CATG 5 cut(s) 42, 616, 859, 895, 1139
Hin6I GCGC 2 cut(s) 482, 775
HinP1I GCGC 2 cut(s) 482, 775
HinfI GANTC 3 cut(s) 99, 455, 490
HpaII CCGG 7 cut(s) 179, 248, 340, 459, 555, 731, 881
HphI GGTGA 3 cut(s) 129, 737, 1090
Hpy166II GTNNAC 6 cut(s) 323, 655, 823, 958, 960, 1052
Hpy188I TCNGA 5 cut(s) 98, 397, 594, 766, 1001
Hpy188III TCNNGA 5 cut(s) 459, 574, 580, 668, 1087
Hpy8I GTNNAC 6 cut(s) 323, 655, 823, 958, 960, 1052
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 3 cut(s) 54, 242, 286
HpyCH4III ACNGT 4 cut(s) 94, 320, 722, 1005
HpyCH4IV ACGT 3 cut(s) 49, 134, 680
HpyCH4V TGCA 4 cut(s) 4, 761, 851, 1052
HpyF10VI GCNNNNNNNGC 6 cut(s) 433, 503, 710, 750, 842, 922
HpyF3I CTNAG 3 cut(s) 332, 507, 714
HpySE526I ACGT 3 cut(s) 49, 134, 680
Hsp92I GRCGYC 2 cut(s) 775, 1140
Hsp92II CATG 5 cut(s) 42, 616, 859, 895, 1139
HspAI GCGC 2 cut(s) 482, 775
KasI GGCGCC 1 cut(s) 774
Kpn2I TCCGGA 1 cut(s) 458
Kzo9I GATC 8 cut(s) 192, 378, 390, 526, 576, 727, 870, 1083
LmnI GCTCC 3 cut(s) 300, 605, 616
Lsp1109I GCAGC 1 cut(s) 339
LweI GCATC 2 cut(s) 668, 770
MaeI CTAG 3 cut(s) 116, 206, 1094
MaeII ACGT 3 cut(s) 49, 134, 680
MaeIII GTNAC 3 cut(s) 50, 135, 201
MalI GATC 8 cut(s) 194, 380, 392, 528, 578, 729, 872, 1085
MboI GATC 8 cut(s) 192, 378, 390, 526, 576, 727, 870, 1083
MboII GAAGA 7 cut(s) 172, 410, 595, 877, 880, 1030, 1109
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 5 cut(s) 331, 438, 476, 927, 1054
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 1 cut(s) 970
MluNI TGGCCA 1 cut(s) 131
Mly113I GGCGCC 1 cut(s) 775
MlyI GAGTC 1 cut(s) 464
Mox20I TGGCCA 1 cut(s) 131
MroI TCCGGA 1 cut(s) 458
MscI TGGCCA 1 cut(s) 131
MseI TTAA 2 cut(s) 197, 1031
MslI CAYNNNNRTG 1 cut(s) 972
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 7 cut(s) 179, 248, 340, 459, 555, 731, 881
MspR9I CCNGG 3 cut(s) 20, 621, 882
MvaI CCWGG 2 cut(s) 20, 621
MwoI GCNNNNNNNGC 6 cut(s) 433, 503, 710, 750, 842, 922
NarI GGCGCC 1 cut(s) 775
NciI CCSGG 1 cut(s) 882
NdeI CATATG 1 cut(s) 746
NdeII GATC 8 cut(s) 192, 378, 390, 526, 576, 727, 870, 1083
NlaIII CATG 5 cut(s) 42, 616, 859, 895, 1139
NlaIV GGNNCC 7 cut(s) 338, 618, 754, 776, 825, 909, 934
NmeAIII GCCGAG 1 cut(s) 685
NmuCI GTSAC 3 cut(s) 50, 135, 201
PaeR7I CTCGAG 1 cut(s) 469
PfeI GAWTC 2 cut(s) 99, 490
PkrI GCNGC 3 cut(s) 354, 752, 1134
PleI GAGTC 1 cut(s) 463
PluTI GGCGCC 1 cut(s) 778
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 1 cut(s) 463
Ppu21I YACGTR 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 907
PsiI TTATAA 1 cut(s) 1161
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 2 cut(s) 18, 619
PspCI CACGTG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 2 cut(s) 18, 619
PspN4I GGNNCC 7 cut(s) 338, 618, 754, 776, 825, 909, 934
PspPI GGNCC 7 cut(s) 216, 316, 337, 501, 823, 907, 933
PspPPI RGGWCCY 1 cut(s) 907
PspXI VCTCGAGB 1 cut(s) 469
PsrI GAACNNNNNNTAC 2 cut(s) 623, 655
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 9 cut(s) 274, 324, 365, 416, 548, 641, 656, 920, 959
RsaNI GTAC 9 cut(s) 273, 323, 364, 415, 547, 640, 655, 919, 958
RseI CAYNNNNRTG 1 cut(s) 972
SaqAI TTAA 2 cut(s) 197, 1031
SatI GCNGC 3 cut(s) 353, 751, 1133
Sau3AI GATC 8 cut(s) 192, 378, 390, 526, 576, 727, 870, 1083
Sau96I GGNCC 7 cut(s) 216, 316, 337, 501, 823, 907, 933
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 3 cut(s) 20, 621, 882
SduI GDGCHC 5 cut(s) 331, 438, 476, 927, 1054
SfaNI GCATC 2 cut(s) 668, 770
SfoI GGCGCC 1 cut(s) 776
Sfr274I CTCGAG 1 cut(s) 469
SinI GGWCC 5 cut(s) 316, 337, 501, 823, 907
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 972
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 1 cut(s) 970
SsiI CCGC 7 cut(s) 123, 504, 517, 742, 750, 977, 1133
SspDI GGCGCC 1 cut(s) 774
SspMI CTAG 3 cut(s) 116, 206, 1094
StyD4I CCNGG 3 cut(s) 18, 619, 880
TaaI ACNGT 4 cut(s) 94, 320, 722, 1005
TaiI ACGT 3 cut(s) 52, 137, 683
TaqI TCGA 5 cut(s) 154, 252, 438, 470, 579
TasI AATT 1 cut(s) 970
TatI WGTACW 2 cut(s) 363, 957
TauI GCSGC 2 cut(s) 753, 1135
TfiI GAWTC 2 cut(s) 99, 490
Tru1I TTAA 2 cut(s) 197, 1031
Tru9I TTAA 2 cut(s) 197, 1031
TscAI CASTG 1 cut(s) 491
TseFI GTSAC 3 cut(s) 50, 135, 201
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 3 cut(s) 50, 135, 201
TspDTI ATGAA 2 cut(s) 673, 1142
TspGWI ACGGA 2 cut(s) 456, 641
TspRI CASTG 1 cut(s) 491
VneI GTGCAC 1 cut(s) 1050
VpaK11BI GGWCC 5 cut(s) 316, 337, 501, 823, 907
XhoI CTCGAG 1 cut(s) 469
XspI CTAG 3 cut(s) 116, 206, 1094
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.