Rroxscaffold_7G00191560

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007963
Physical Location & Seq
Forward (+)
32194097 .. 32195898
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00191560.1

Sequence Viewer

Length: 1182 bp
ATGCAAGCCTCCCTCTGCCTGGACACCAAGACTCTCGCATGGCCTTCACGTGGCCACACCTCAACCCACCACATATTCCGGCCAACACGCACCGTCAGATTCAGGCCAATGCCATCAGTCTCCGCCGTGGCCACGTCACCTCCGCCTCTAAATCGGCAGAAGAGCCACTCTTTGCCACCGGAGAAGGTGGAGCTCTTCAAGTCACTAGAAGGCTGGGCCTCCGAGACAATCCTCCCATTTCTGAAGCCGGTGGACGAATGCTGGCAACCCAGTAACTTCTTGCCGGACCCGTCGCTCCCTAGAGAGGAGTTCTTGGACCGGGTTCGGGCACTTAGGGACCGGACCGATGGGCTGCCCGACGAGTACTTTGTGGTGCTGGTCGGGGACATGATCACCGAGGAGGCGTTGCCTACGTACCAGAGTATGATCAATGGGCTCGATGGGGTGAGAGACGAGACCGGAGCTAGCTCGAGCCCATGGGCACGGTGGACTCGGGCTTGGACCGCTGAGGAAAACCGCCACGGGGATCTGCTCAAAACCTATCTGTACTTGTCCGGTCGGGTTGATATGTTCAGGATCGAGAAGACTATTCAGAACCTTATTGGAGCTGGCATGGAGCCAGGGACGGAGAATAACCCGTACTTGGGTTTCGTGTACACTTCATTTCAAGAGCGAGCCACGTTCATATCGCATGGCAACACGGCTCGGCTGGCTAAGGACCGTGGCGATCCGGTGCTGGCCCGCATATGCGGCACCATTGCATCCGACGAGAGGCGCCACGAGTTCGCCTATTCCAAGATTGTTGAAAAGCTACTCGAAGTGGACTCAACCGGAGCAATGATCGCCATTGCGGACATGATGAGGAAGAAGATCACAATGCCGGCCCATCTTATGTACGATGGGGAGGACCCCAAGCTGTTCGAGCATTTTGCGGCTGTGGCTCAACGGCTTGGGGTGTACACGGCACATGATTATGCGGACATTTTGGAGTTCTTGATCGGACGGTGGAGGCTGGAGAAGGTGGAAGGATTGACGGGGGAGGGTAAGCGTGCACAGGACTTCGTGTGTGGGTTGGCGCCGAGGATCAGGAGGCTAGAAGAGCGGGCCGATGAGCGAGCAAGGAAGATGGAGCCGCATGGCGTCAAGTTTAGCTGGATTTATAATAAAGAAGTGGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

393

Amino Acids

44.74

Weight (kDa)

6.44

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 61 - 386 6.2e-153 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AccB1I GGYRCC 3 cut(s) 752, 774, 1075
AccBSI CCGCTC 1 cut(s) 1102
AclWI GGATC 4 cut(s) 534, 584, 722, 1091
AcoI YGGCCR 3 cut(s) 52, 80, 129
AcuI CTGAAG 1 cut(s) 263
AcvI CACGTG 1 cut(s) 50
AcyI GRCGYC 3 cut(s) 775, 1076, 1140
AfaI GTAC 7 cut(s) 365, 416, 548, 641, 656, 896, 959
AfiI CCNNNNNNNGG 8 cut(s) 19, 50, 304, 325, 523, 643, 644, 771
AgsI TTSAA 3 cut(s) 199, 668, 806
AjiI CACGTC 1 cut(s) 135
AjnI CCWGG 2 cut(s) 18, 619
AluBI AGCT 7 cut(s) 193, 464, 468, 608, 811, 916, 1152
AluI AGCT 7 cut(s) 193, 464, 468, 608, 811, 916, 1152
Alw21I GWGCWC 2 cut(s) 195, 1054
Alw26I GTCTC 4 cut(s) 124, 218, 444, 449
Alw44I GTGCAC 1 cut(s) 1050
AlwI GGATC 4 cut(s) 534, 584, 722, 1091
Ama87I CYCGRG 2 cut(s) 469, 492
AoxI GGCC 9 cut(s) 41, 52, 80, 104, 129, 216, 738, 882, 1104
ApaLI GTGCAC 1 cut(s) 1050
ApeKI GCWGC 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 2 cut(s) 777, 1078
AsuC2I CCSGG 1 cut(s) 320
AsuHPI GGTGA 3 cut(s) 129, 385, 457
AsuNHI GCTAGC 1 cut(s) 464
AvaI CYCGRG 2 cut(s) 469, 492
AvaII GGWCC 7 cut(s) 286, 316, 337, 342, 501, 718, 907
BaeGI GKGCMC 3 cut(s) 331, 484, 1054
BalI TGGCCA 2 cut(s) 54, 131
BanI GGYRCC 3 cut(s) 752, 774, 1075
BanII GRGCYC 3 cut(s) 195, 438, 476
BauI CACGAG 1 cut(s) 779
BbrPI CACGTG 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 590
Bbv12I GWGCWC 2 cut(s) 195, 1054
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 1 cut(s) 339
BccI CCATC 6 cut(s) 121, 341, 434, 893, 894, 1120
BceAI ACGGC 4 cut(s) 110, 717, 962, 978
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 2 cut(s) 20, 621
BclI TGATCA 2 cut(s) 390, 426
BcnI CCSGG 1 cut(s) 320
BcoDI GTCTC 4 cut(s) 124, 218, 444, 449
BfaI CTAG 4 cut(s) 206, 300, 465, 1094
BfoI RGCGCY 2 cut(s) 778, 1079
BisI GCNGC 4 cut(s) 353, 751, 933, 1133
BlsI GCNGC 4 cut(s) 354, 752, 934, 1134
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 3 cut(s) 20, 320, 621
Bme18I GGWCC 7 cut(s) 286, 316, 337, 342, 501, 718, 907
BmeT110I CYCGRG 2 cut(s) 469, 492
BmgBI CACGTC 1 cut(s) 135
BmiI GGNNCC 8 cut(s) 288, 338, 618, 754, 776, 909, 1077, 1131
BmrFI CCNGG 3 cut(s) 20, 320, 621
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 770
BmtI GCTAGC 1 cut(s) 468
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 590
BpmI CTGGAG 1 cut(s) 1034
Bpu10I CCTNAGC 2 cut(s) 507, 714
BpuMI CCSGG 1 cut(s) 320
BsaAI YACGTR 2 cut(s) 50, 414
BsaHI GRCGYC 3 cut(s) 775, 1076, 1140
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 7 cut(s) 126, 396, 476, 520, 620, 721, 1079
BsaWI WCCGGW 6 cut(s) 178, 339, 458, 554, 730, 830
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 8 cut(s) 19, 50, 304, 325, 523, 643, 644, 771
Bse118I RCCGGY 2 cut(s) 247, 880
Bse1I ACTGG 1 cut(s) 270
Bse3DI GCAATG 3 cut(s) 756, 843, 846
BseBI CCWGG 2 cut(s) 20, 621
BseDI CCNNGG 7 cut(s) 126, 396, 476, 520, 620, 721, 1079
BseGI GGATG 1 cut(s) 761
BseLI CCNNNNNNNGG 8 cut(s) 19, 50, 304, 325, 523, 643, 644, 771
BseMI GCAATG 3 cut(s) 756, 843, 846
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 2 cut(s) 320, 413
BseSI GKGCMC 3 cut(s) 331, 484, 1054
BseXI GCAGC 1 cut(s) 339
BseYI CCCAGC 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 9 cut(s) 43, 54, 82, 106, 131, 218, 740, 884, 1106
BshNI GGYRCC 3 cut(s) 752, 774, 1075
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 2 cut(s) 195, 1054
BsiHKCI CYCGRG 2 cut(s) 469, 492
BslFI GGGAC 3 cut(s) 350, 398, 637
BslI CCNNNNNNNGG 8 cut(s) 19, 50, 304, 325, 523, 643, 644, 771
BsmAI GTCTC 4 cut(s) 124, 218, 444, 449
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 3 cut(s) 350, 398, 637
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 9 cut(s) 43, 54, 82, 106, 131, 218, 740, 884, 1106
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 2 cut(s) 469, 492
Bsp1286I GDGCHC 6 cut(s) 195, 331, 438, 476, 484, 1054
Bsp1407I TGTACA 2 cut(s) 654, 957
Bsp143I GATC 9 cut(s) 390, 426, 526, 576, 727, 840, 870, 996, 1083
Bsp19I CCATGG 1 cut(s) 476
BspANI GGCC 9 cut(s) 43, 54, 82, 106, 131, 218, 740, 884, 1106
BspCNI CTCAG 1 cut(s) 499
BspLI GGNNCC 8 cut(s) 288, 338, 618, 754, 776, 909, 1077, 1131
BspOI GCTAGC 1 cut(s) 468
BspPI GGATC 4 cut(s) 534, 584, 722, 1091
BspQI GCTCTTC 3 cut(s) 155, 200, 1092
BspT107I GGYRCC 3 cut(s) 752, 774, 1075
BspTNI GGTCTC 1 cut(s) 449
BsrBI CCGCTC 1 cut(s) 1102
BsrDI GCAATG 3 cut(s) 756, 843, 846
BsrFI RCCGGY 2 cut(s) 247, 880
BsrGI TGTACA 2 cut(s) 654, 957
BsrI ACTGG 1 cut(s) 270
BssAI RCCGGY 2 cut(s) 247, 880
BssECI CCNNGG 7 cut(s) 126, 396, 476, 520, 620, 721, 1079
BssMI GATC 9 cut(s) 390, 426, 526, 576, 727, 840, 870, 996, 1083
BssNI GRCGYC 3 cut(s) 775, 1076, 1140
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 2 cut(s) 20, 621
Bst4CI ACNGT 4 cut(s) 94, 486, 722, 1005
Bst6I CTCTTC 3 cut(s) 155, 200, 1092
BstACI GRCGYC 3 cut(s) 775, 1076, 1140
BstAUI TGTACA 2 cut(s) 654, 957
BstBAI YACGTR 2 cut(s) 50, 414
BstDEI CTNAG 3 cut(s) 332, 507, 714
BstDSI CCRYGG 4 cut(s) 126, 476, 520, 721
BstF5I GGATG 1 cut(s) 761
BstH2I RGCGCY 2 cut(s) 778, 1079
BstHHI GCGC 2 cut(s) 777, 1078
BstKTI GATC 9 cut(s) 393, 429, 529, 579, 730, 843, 873, 999, 1086
BstMAI GTCTC 4 cut(s) 124, 218, 444, 449
BstMBI GATC 9 cut(s) 390, 426, 526, 576, 727, 840, 870, 996, 1083
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 7 cut(s) 503, 710, 750, 842, 922, 938, 1099
BstNI CCWGG 2 cut(s) 20, 621
BstSCI CCNGG 3 cut(s) 18, 318, 619
BstSLI GKGCMC 3 cut(s) 331, 484, 1054
BstSNI TACGTA 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 339
BstV2I GAAGAC 1 cut(s) 590
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 9 cut(s) 43, 54, 82, 106, 131, 218, 740, 884, 1106
BtgI CCRYGG 4 cut(s) 126, 476, 520, 721
BtrI CACGTC 1 cut(s) 135
BtsCI GGATG 1 cut(s) 761
CfoI GCGC 2 cut(s) 777, 1078
Cfr10I RCCGGY 2 cut(s) 247, 880
CpoI CGGWCCG 1 cut(s) 342
CseI GACGC 1 cut(s) 1129
Csp6I GTAC 7 cut(s) 364, 415, 547, 640, 655, 895, 958
CspI CGGWCCG 1 cut(s) 342
CviAII CATG 8 cut(s) 39, 388, 477, 613, 692, 856, 968, 1136
CviQI GTAC 7 cut(s) 364, 415, 547, 640, 655, 895, 958
DdeI CTNAG 3 cut(s) 332, 507, 714
DinI GGCGCC 2 cut(s) 776, 1077
DpnI GATC 9 cut(s) 392, 428, 528, 578, 729, 842, 872, 998, 1085
DpnII GATC 9 cut(s) 390, 426, 526, 576, 727, 840, 870, 996, 1083
EaeI YGGCCR 3 cut(s) 52, 80, 129
Eam1104I CTCTTC 3 cut(s) 155, 200, 1092
EarI CTCTTC 3 cut(s) 155, 200, 1092
EciI GGCGGA 2 cut(s) 112, 132
Ecl136II GAGCTC 1 cut(s) 193
Eco105I TACGTA 1 cut(s) 414
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 3 cut(s) 195, 438, 476
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 7 cut(s) 286, 316, 337, 342, 501, 718, 907
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 263
Eco72I CACGTG 1 cut(s) 50
Eco88I CYCGRG 2 cut(s) 469, 492
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 2 cut(s) 18, 619
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 3 cut(s) 195, 438, 476
EgeI GGCGCC 2 cut(s) 776, 1077
EheI GGCGCC 2 cut(s) 776, 1077
ErhI CCWWGG 1 cut(s) 476
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 8 cut(s) 42, 391, 480, 616, 695, 859, 971, 1139
FaqI GGGAC 3 cut(s) 350, 398, 637
FatI CATG 8 cut(s) 38, 387, 476, 612, 691, 855, 967, 1135
FauI CCCGC 2 cut(s) 749, 1095
FauNDI CATATG 1 cut(s) 746
FbaI TGATCA 2 cut(s) 390, 426
Fnu4HI GCNGC 4 cut(s) 353, 751, 933, 1133
FokI GGATG 1 cut(s) 748
FriOI GRGCYC 3 cut(s) 195, 438, 476
Fsp4HI GCNGC 4 cut(s) 353, 751, 933, 1133
FspBI CTAG 4 cut(s) 206, 300, 465, 1094
GlaI GCGC 2 cut(s) 776, 1077
GluI GCNGC 4 cut(s) 353, 751, 933, 1133
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 1034
HaeII RGCGCY 2 cut(s) 778, 1079
HaeIII GGCC 9 cut(s) 43, 54, 82, 106, 131, 218, 740, 884, 1106
HgaI GACGC 1 cut(s) 1129
HhaI GCGC 2 cut(s) 777, 1078
Hin1I GRCGYC 3 cut(s) 775, 1076, 1140
Hin1II CATG 8 cut(s) 42, 391, 480, 616, 695, 859, 971, 1139
Hin6I GCGC 2 cut(s) 775, 1076
HinP1I GCGC 2 cut(s) 775, 1076
HinfI GANTC 4 cut(s) 31, 99, 490, 824
HphI GGTGA 3 cut(s) 129, 385, 457
Hpy166II GTNNAC 8 cut(s) 253, 489, 655, 657, 823, 958, 960, 1052
Hpy188I TCNGA 6 cut(s) 98, 223, 243, 594, 766, 1001
Hpy188III TCNNGA 5 cut(s) 574, 580, 668, 994, 1087
Hpy8I GTNNAC 8 cut(s) 253, 489, 655, 657, 823, 958, 960, 1052
Hpy99I CGWCG 3 cut(s) 295, 362, 770
HpyAV CCTTC 5 cut(s) 54, 178, 203, 1012, 1019
HpyCH4III ACNGT 4 cut(s) 94, 486, 722, 1005
HpyCH4IV ACGT 4 cut(s) 49, 134, 413, 680
HpyCH4V TGCA 3 cut(s) 4, 761, 1052
HpyF10VI GCNNNNNNNGC 7 cut(s) 503, 710, 750, 842, 922, 938, 1099
HpyF3I CTNAG 3 cut(s) 332, 507, 714
HpySE526I ACGT 4 cut(s) 49, 134, 413, 680
Hsp92I GRCGYC 3 cut(s) 775, 1076, 1140
Hsp92II CATG 8 cut(s) 42, 391, 480, 616, 695, 859, 971, 1139
HspAI GCGC 2 cut(s) 775, 1076
KasI GGCGCC 2 cut(s) 774, 1075
KroI GCCGGC 1 cut(s) 880
KroNI GCCGGC 1 cut(s) 882
Ksp22I TGATCA 2 cut(s) 390, 426
Kzo9I GATC 9 cut(s) 390, 426, 526, 576, 727, 840, 870, 996, 1083
LguI GCTCTTC 3 cut(s) 155, 200, 1092
LmnI GCTCC 7 cut(s) 190, 300, 461, 605, 616, 833, 1129
Lsp1109I GCAGC 1 cut(s) 339
LweI GCATC 1 cut(s) 770
MaeI CTAG 4 cut(s) 206, 300, 465, 1094
MaeII ACGT 4 cut(s) 49, 134, 413, 680
MaeIII GTNAC 3 cut(s) 135, 201, 272
MalI GATC 9 cut(s) 392, 428, 528, 578, 729, 842, 872, 998, 1085
MbiI CCGCTC 1 cut(s) 1102
MboI GATC 9 cut(s) 390, 426, 526, 576, 727, 840, 870, 996, 1083
MboII GAAGA 7 cut(s) 172, 187, 595, 877, 880, 1109, 1135
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 6 cut(s) 195, 331, 438, 476, 484, 1054
MlsI TGGCCA 2 cut(s) 54, 131
MluNI TGGCCA 2 cut(s) 54, 131
Mly113I GGCGCC 2 cut(s) 775, 1076
MlyI GAGTC 3 cut(s) 25, 484, 818
MmeI TCCRAC 1 cut(s) 789
Mox20I TGGCCA 2 cut(s) 54, 131
MroNI GCCGGC 1 cut(s) 880
MscI TGGCCA 2 cut(s) 54, 131
MslI CAYNNNNRTG 1 cut(s) 972
Msp20I TGGCCA 2 cut(s) 54, 131
MspA1I CMGCKG 1 cut(s) 506
MspR9I CCNGG 3 cut(s) 20, 320, 621
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 2 cut(s) 20, 621
MwoI GCNNNNNNNGC 7 cut(s) 503, 710, 750, 842, 922, 938, 1099
NaeI GCCGGC 1 cut(s) 882
NarI GGCGCC 2 cut(s) 775, 1076
NciI CCSGG 1 cut(s) 320
NcoI CCATGG 1 cut(s) 476
NdeI CATATG 1 cut(s) 746
NdeII GATC 9 cut(s) 390, 426, 526, 576, 727, 840, 870, 996, 1083
NgoMIV GCCGGC 1 cut(s) 880
NheI GCTAGC 1 cut(s) 464
NlaIII CATG 8 cut(s) 42, 391, 480, 616, 695, 859, 971, 1139
NlaIV GGNNCC 8 cut(s) 288, 338, 618, 754, 776, 909, 1077, 1131
NmeAIII GCCGAG 2 cut(s) 685, 1104
NmuCI GTSAC 2 cut(s) 135, 201
PaeR7I CTCGAG 1 cut(s) 469
PciSI GCTCTTC 3 cut(s) 155, 200, 1092
PctI GAATGC 1 cut(s) 263
PdiI GCCGGC 1 cut(s) 882
PfeI GAWTC 1 cut(s) 99
PkrI GCNGC 4 cut(s) 354, 752, 934, 1134
PleI GAGTC 3 cut(s) 25, 484, 818
PluTI GGCGCC 2 cut(s) 778, 1079
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 3 cut(s) 25, 484, 818
Ppu21I YACGTR 2 cut(s) 50, 414
PpuMI RGGWCCY 1 cut(s) 907
PsiI TTATAA 1 cut(s) 1161
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 2 cut(s) 18, 619
PspCI CACGTG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 2 cut(s) 18, 619
PspN4I GGNNCC 8 cut(s) 288, 338, 618, 754, 776, 909, 1077, 1131
PspPPI RGGWCCY 1 cut(s) 907
PspXI VCTCGAGB 1 cut(s) 469
PsuI RGATCY 1 cut(s) 526
RsaI GTAC 7 cut(s) 365, 416, 548, 641, 656, 896, 959
RsaNI GTAC 7 cut(s) 364, 415, 547, 640, 655, 895, 958
RseI CAYNNNNRTG 1 cut(s) 972
Rsr2I CGGWCCG 1 cut(s) 342
RsrII CGGWCCG 1 cut(s) 342
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 3 cut(s) 155, 200, 1092
SatI GCNGC 4 cut(s) 353, 751, 933, 1133
Sau3AI GATC 9 cut(s) 390, 426, 526, 576, 727, 840, 870, 996, 1083
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 3 cut(s) 25, 484, 818
ScrFI CCNGG 3 cut(s) 20, 320, 621
SduI GDGCHC 6 cut(s) 195, 331, 438, 476, 484, 1054
SfaNI GCATC 1 cut(s) 770
SfoI GGCGCC 2 cut(s) 776, 1077
Sfr274I CTCGAG 1 cut(s) 469
SinI GGWCC 7 cut(s) 286, 316, 337, 342, 501, 718, 907
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 972
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
SnaBI TACGTA 1 cut(s) 414
SspDI GGCGCC 2 cut(s) 774, 1075
SspMI CTAG 4 cut(s) 206, 300, 465, 1094
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 3 cut(s) 18, 318, 619
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 4 cut(s) 94, 486, 722, 1005
TaiI ACGT 4 cut(s) 52, 137, 416, 683
TaqI TCGA 5 cut(s) 438, 470, 579, 816, 921
TaqII GACCGA 1 cut(s) 359
TatI WGTACW 4 cut(s) 363, 546, 654, 957
TauI GCSGC 3 cut(s) 753, 935, 1135
TfiI GAWTC 1 cut(s) 99
TseFI GTSAC 2 cut(s) 135, 201
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 2 cut(s) 135, 201
TspDTI ATGAA 2 cut(s) 651, 673
TspGWI ACGGA 1 cut(s) 641
VneI GTGCAC 1 cut(s) 1050
VpaK11BI GGWCC 7 cut(s) 286, 316, 337, 342, 501, 718, 907
XcmI CCANNNNNNNNNTGG 1 cut(s) 483
XhoI CTCGAG 1 cut(s) 469
XspI CTAG 4 cut(s) 206, 300, 465, 1094
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.