RLG00000013318

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007963
Physical Location & Seq
Forward (+)
29789167 .. 29793536
4370 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013318

Sequence Viewer

Length: 1182 bp
ATGCAAGCCTCCTTCTACCTGGACACCAAAACTCTCGCATGGCCTTCACGTGGCCACACCTCAACCCACCACATATTCTGGCCAACAACCACAGTCAGATTCAGGCTATTGCCTCTAGTCTCCGCCGTGGCCATGTCACCTCCACCTCTAACTCGGCAGAAGACCCACTCGTTGCCACCGGTGAAGGTGGAGCTCTTCAAGTCACTAGAGGGCTGGGCCTCCCAGACAATCCTCCCATTTCTGAAGCCGGTCGATGAGTGCTGGCAACCCAGTACCTTCTTGCCTGACCCATCGCTCCTCAAAGAGGAGTTCTTGGACCGTGTACGGGCACTTAGGGACCGGACAGATGGGCTGCCCGATGAGTACTTTGTGGTGCTGGTCGGGGATATGATCAGCGAGGAGGTGTTGCCTGCTTACCAGAGTATGCTTAATGGGCTCGATGGGGTGAGAGACGAGTCCGGGGCTAGCTCGAGCCCGTGGGCGCAGTGGATTCAGGCTTGGTCCTCTGAGGAAAACCGCCACGGCGATCTACTCAAAACCTTTCTGTACCTATCCGGTCGGGTTGATATGTTCAAGATCGAGAAGACTATTCAGAACCTAATTGGAGCTGGCATGGAGCCAGGAACGGAGAACAACCCGTACTTGAGTTTCGTGTATGCATCATTTCAAGAGCGAGCCACGTTCATATCGCACGGCAACACAGCTCGACTGGCTAAGGACTGCGGTGATCTGGTGCTTGCCCGCATATGCGGCACCATTGCATCCGATGAGAGGCGCCACGAGTTCGCCTATTCCAAGATTGTAGAAAAGCTACTTGAAGTGGACTCGGCTAGTGCAATGGTCGCCATTGCGGACATGATGAGGAAGAAGATCACAATGCCGGGTCATCTCATGTATGATGGGGAGGACCCCAAGCTCTACGAGCACTTTGGGGCCGTGGCTCAACGGCTTGGGGTGTACACAACACATGATTATGCAGACATTTTGGAGTTTTTTATCGGACGGTGGAGGCTGGAGAAGATGGAGGGATTGACGGGAGAGGGTAAGCGTGCACAAGACTTTGTGTGTGGGTTGTTGGTGAGGATAAGGAGGCTAGAAGAGCGGGCCGATGAGCGAGCAAGGAAGATGAAACCGCATGGCATTAAGTTTAGCTGGATTTATAATAAAGAAGTGGTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

394

Amino Acids

44.78

Weight (kDa)

6.29

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 62 - 386 4.3e-143 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AccB1I GGYRCC 2 cut(s) 752, 774
AccBSI CCGCTC 1 cut(s) 1102
AciI CCGC 8 cut(s) 123, 517, 723, 742, 750, 851, 1102, 1133
AcoI YGGCCR 3 cut(s) 52, 80, 129
AcuI CTGAAG 1 cut(s) 263
AcvI CACGTG 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 775
AfaI GTAC 6 cut(s) 274, 324, 365, 548, 641, 959
AfiI CCNNNNNNNGG 4 cut(s) 50, 304, 325, 771
AgeI ACCGGT 1 cut(s) 178
AgsI TTSAA 4 cut(s) 199, 574, 668, 818
AjnI CCWGG 2 cut(s) 18, 619
AluBI AGCT 7 cut(s) 193, 468, 608, 704, 811, 916, 1152
AluI AGCT 7 cut(s) 193, 468, 608, 704, 811, 916, 1152
Alw21I GWGCWC 3 cut(s) 195, 927, 1054
Alw26I GTCTC 2 cut(s) 124, 444
Alw44I GTGCAC 1 cut(s) 1050
Ama87I CYCGRG 1 cut(s) 469
AoxI GGCC 7 cut(s) 41, 52, 80, 129, 216, 933, 1104
ApaLI GTGCAC 1 cut(s) 1050
ApeKI GCWGC 1 cut(s) 352
AsiGI ACCGGT 1 cut(s) 178
AspLEI GCGC 2 cut(s) 484, 777
AspS9I GGNCC 7 cut(s) 216, 316, 337, 501, 907, 933, 1104
AsuC2I CCSGG 2 cut(s) 460, 882
AsuHPI GGTGA 5 cut(s) 129, 193, 457, 737, 1090
AsuNHI GCTAGC 1 cut(s) 464
AvaI CYCGRG 1 cut(s) 469
AvaII GGWCC 4 cut(s) 316, 337, 501, 907
BaeGI GKGCMC 2 cut(s) 331, 1054
BalI TGGCCA 3 cut(s) 54, 82, 131
BanI GGYRCC 2 cut(s) 752, 774
BanII GRGCYC 3 cut(s) 195, 438, 476
BauI CACGAG 1 cut(s) 779
BbrPI CACGTG 1 cut(s) 50
BbsI GAAGAC 2 cut(s) 167, 590
Bbv12I GWGCWC 3 cut(s) 195, 927, 1054
BbvI GCAGC 1 cut(s) 339
BccI CCATC 5 cut(s) 298, 341, 434, 893, 1015
BceAI ACGGC 5 cut(s) 110, 538, 709, 920, 962
BciT130I CCWGG 2 cut(s) 20, 621
BclI TGATCA 1 cut(s) 390
BcnI CCSGG 2 cut(s) 460, 882
BcoDI GTCTC 2 cut(s) 124, 444
BfaI CTAG 5 cut(s) 116, 206, 465, 831, 1094
BfoI RGCGCY 1 cut(s) 778
BisI GCNGC 2 cut(s) 353, 751
BlsI GCNGC 2 cut(s) 354, 752
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 4 cut(s) 20, 460, 621, 882
Bme18I GGWCC 4 cut(s) 316, 337, 501, 907
BmeT110I CYCGRG 1 cut(s) 469
BmgT120I GGNCC 7 cut(s) 216, 316, 337, 501, 907, 933, 1104
BmiI GGNNCC 6 cut(s) 338, 618, 754, 776, 909, 934
BmrFI CCNGG 4 cut(s) 20, 460, 621, 882
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 668, 770
BmtI GCTAGC 1 cut(s) 468
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 167, 590
BpmI CTGGAG 1 cut(s) 1034
Bpu10I CCTNAGC 1 cut(s) 714
BpuEI CTTGAG 1 cut(s) 664
BpuMI CCSGG 2 cut(s) 460, 882
BsaAI YACGTR 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 775
BsaJI CCNNGG 5 cut(s) 126, 459, 476, 520, 936
BsaWI WCCGGW 3 cut(s) 178, 339, 554
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 304, 325, 771
Bse118I RCCGGY 2 cut(s) 178, 247
Bse1I ACTGG 2 cut(s) 270, 714
Bse3DI GCAATG 3 cut(s) 756, 843, 846
BseBI CCWGG 2 cut(s) 20, 621
BseDI CCNNGG 5 cut(s) 126, 459, 476, 520, 936
BseGI GGATG 1 cut(s) 761
BseLI CCNNNNNNNGG 4 cut(s) 50, 304, 325, 771
BseMI GCAATG 3 cut(s) 756, 843, 846
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 2 cut(s) 270, 714
BseRI GAGGAG 3 cut(s) 287, 320, 413
BseSI GKGCMC 2 cut(s) 331, 1054
BseXI GCAGC 1 cut(s) 339
BseYI CCCAGC 1 cut(s) 213
Bsh1285I CGRYCG 2 cut(s) 252, 559
BshFI GGCC 7 cut(s) 43, 54, 82, 131, 218, 935, 1106
BshNI GGYRCC 2 cut(s) 752, 774
BshTI ACCGGT 1 cut(s) 178
BsiEI CGRYCG 2 cut(s) 252, 559
BsiHKAI GWGCWC 3 cut(s) 195, 927, 1054
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 6 cut(s) 179, 248, 340, 459, 555, 881
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 4 cut(s) 50, 304, 325, 771
BsmAI GTCTC 2 cut(s) 124, 444
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 7 cut(s) 43, 54, 82, 131, 218, 935, 1106
BsoBI CYCGRG 1 cut(s) 469
Bsp1286I GDGCHC 6 cut(s) 195, 331, 438, 476, 927, 1054
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 5 cut(s) 390, 526, 576, 727, 870
BspACI CCGC 8 cut(s) 123, 517, 723, 742, 750, 851, 1102, 1133
BspANI GGCC 7 cut(s) 43, 54, 82, 131, 218, 935, 1106
BspCNI CTCAG 1 cut(s) 499
BspLI GGNNCC 6 cut(s) 338, 618, 754, 776, 909, 934
BspOI GCTAGC 1 cut(s) 468
BspQI GCTCTTC 2 cut(s) 200, 1092
BspT107I GGYRCC 2 cut(s) 752, 774
BsrBI CCGCTC 1 cut(s) 1102
BsrDI GCAATG 3 cut(s) 756, 843, 846
BsrFI RCCGGY 2 cut(s) 178, 247
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 2 cut(s) 270, 714
BssAI RCCGGY 2 cut(s) 178, 247
BssECI CCNNGG 5 cut(s) 126, 459, 476, 520, 936
BssMI GATC 5 cut(s) 390, 526, 576, 727, 870
BssNI GRCGYC 1 cut(s) 775
BssSI CACGAG 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 2 cut(s) 20, 621
Bst4CI ACNGT 3 cut(s) 94, 320, 1005
Bst6I CTCTTC 2 cut(s) 200, 1092
BstACI GRCGYC 1 cut(s) 775
BstAUI TGTACA 1 cut(s) 957
BstBAI YACGTR 1 cut(s) 50
BstDEI CTNAG 3 cut(s) 332, 507, 714
BstDSI CCRYGG 4 cut(s) 126, 476, 520, 936
BstENI CCTNNNNNAGG 1 cut(s) 302
BstF5I GGATG 1 cut(s) 761
BstH2I RGCGCY 1 cut(s) 778
BstHHI GCGC 2 cut(s) 484, 777
BstKTI GATC 5 cut(s) 393, 529, 579, 730, 873
BstMAI GTCTC 2 cut(s) 124, 444
BstMBI GATC 5 cut(s) 390, 526, 576, 727, 870
BstMCI CGRYCG 2 cut(s) 252, 559
BstMWI GCNNNNNNNGC 6 cut(s) 433, 710, 750, 842, 922, 1099
BstNI CCWGG 2 cut(s) 20, 621
BstSCI CCNGG 4 cut(s) 18, 458, 619, 880
BstSLI GKGCMC 2 cut(s) 331, 1054
BstV1I GCAGC 1 cut(s) 339
BstV2I GAAGAC 2 cut(s) 167, 590
BsuRI GGCC 7 cut(s) 43, 54, 82, 131, 218, 935, 1106
BtgI CCRYGG 4 cut(s) 126, 476, 520, 936
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 1 cut(s) 761
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 1 cut(s) 491
CfoI GCGC 2 cut(s) 484, 777
Cfr10I RCCGGY 2 cut(s) 178, 247
Cfr13I GGNCC 7 cut(s) 216, 316, 337, 501, 907, 933, 1104
Csp6I GTAC 6 cut(s) 273, 323, 364, 547, 640, 958
CspAI ACCGGT 1 cut(s) 178
CviAII CATG 7 cut(s) 39, 133, 613, 856, 892, 968, 1136
CviQI GTAC 6 cut(s) 273, 323, 364, 547, 640, 958
DdeI CTNAG 3 cut(s) 332, 507, 714
DinI GGCGCC 1 cut(s) 776
DpnI GATC 5 cut(s) 392, 528, 578, 729, 872
DpnII GATC 5 cut(s) 390, 526, 576, 727, 870
EaeI YGGCCR 3 cut(s) 52, 80, 129
Eam1104I CTCTTC 2 cut(s) 200, 1092
EarI CTCTTC 2 cut(s) 200, 1092
EciI GGCGGA 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 3 cut(s) 195, 438, 476
Eco47I GGWCC 4 cut(s) 316, 337, 501, 907
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 263
Eco72I CACGTG 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 2 cut(s) 18, 619
EcoT22I ATGCAT 1 cut(s) 661
EcoT38I GRGCYC 3 cut(s) 195, 438, 476
EgeI GGCGCC 1 cut(s) 776
EheI GGCGCC 1 cut(s) 776
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 7 cut(s) 42, 136, 616, 859, 895, 971, 1139
FaqI GGGAC 1 cut(s) 350
FatI CATG 7 cut(s) 38, 132, 612, 855, 891, 967, 1135
FauI CCCGC 2 cut(s) 749, 1095
FauNDI CATATG 1 cut(s) 746
FbaI TGATCA 1 cut(s) 390
Fnu4HI GCNGC 2 cut(s) 353, 751
FokI GGATG 1 cut(s) 748
FriOI GRGCYC 3 cut(s) 195, 438, 476
Fsp4HI GCNGC 2 cut(s) 353, 751
FspBI CTAG 5 cut(s) 116, 206, 465, 831, 1094
GlaI GCGC 2 cut(s) 483, 776
GluI GCNGC 2 cut(s) 353, 751
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 1034
HaeII RGCGCY 1 cut(s) 778
HaeIII GGCC 7 cut(s) 43, 54, 82, 131, 218, 935, 1106
HapII CCGG 6 cut(s) 179, 248, 340, 459, 555, 881
HhaI GCGC 2 cut(s) 484, 777
Hin1I GRCGYC 1 cut(s) 775
Hin1II CATG 7 cut(s) 42, 136, 616, 859, 895, 971, 1139
Hin6I GCGC 2 cut(s) 482, 775
HinP1I GCGC 2 cut(s) 482, 775
HinfI GANTC 4 cut(s) 99, 455, 490, 824
HpaII CCGG 6 cut(s) 179, 248, 340, 459, 555, 881
HphI GGTGA 5 cut(s) 129, 193, 457, 737, 1090
Hpy166II GTNNAC 5 cut(s) 323, 823, 958, 960, 1052
Hpy188I TCNGA 6 cut(s) 98, 243, 508, 594, 766, 1001
Hpy188III TCNNGA 3 cut(s) 574, 580, 668
Hpy8I GTNNAC 5 cut(s) 323, 823, 958, 960, 1052
HpyAV CCTTC 4 cut(s) 22, 54, 178, 286
HpyCH4III ACNGT 3 cut(s) 94, 320, 1005
HpyCH4IV ACGT 2 cut(s) 49, 680
HpyCH4V TGCA 6 cut(s) 4, 659, 761, 836, 977, 1052
HpyF10VI GCNNNNNNNGC 6 cut(s) 433, 710, 750, 842, 922, 1099
HpyF3I CTNAG 3 cut(s) 332, 507, 714
HpySE526I ACGT 2 cut(s) 49, 680
Hsp92I GRCGYC 1 cut(s) 775
Hsp92II CATG 7 cut(s) 42, 136, 616, 859, 895, 971, 1139
HspAI GCGC 2 cut(s) 482, 775
KasI GGCGCC 1 cut(s) 774
Ksp22I TGATCA 1 cut(s) 390
Kzo9I GATC 5 cut(s) 390, 526, 576, 727, 870
LguI GCTCTTC 2 cut(s) 200, 1092
LmnI GCTCC 4 cut(s) 190, 300, 605, 616
Lsp1109I GCAGC 1 cut(s) 339
LweI GCATC 2 cut(s) 668, 770
MaeI CTAG 5 cut(s) 116, 206, 465, 831, 1094
MaeII ACGT 2 cut(s) 49, 680
MaeIII GTNAC 2 cut(s) 135, 201
MalI GATC 5 cut(s) 392, 528, 578, 729, 872
MbiI CCGCTC 1 cut(s) 1102
MboI GATC 5 cut(s) 390, 526, 576, 727, 870
MboII GAAGA 8 cut(s) 172, 187, 595, 877, 880, 1030, 1109, 1135
MhlI GDGCHC 6 cut(s) 195, 331, 438, 476, 927, 1054
MlsI TGGCCA 3 cut(s) 54, 82, 131
MluCI AATT 1 cut(s) 600
MluNI TGGCCA 3 cut(s) 54, 82, 131
Mly113I GGCGCC 1 cut(s) 775
MlyI GAGTC 2 cut(s) 464, 818
Mox20I TGGCCA 3 cut(s) 54, 82, 131
Mph1103I ATGCAT 1 cut(s) 661
MscI TGGCCA 3 cut(s) 54, 82, 131
MseI TTAA 2 cut(s) 429, 1143
MslI CAYNNNNRTG 1 cut(s) 972
Msp20I TGGCCA 3 cut(s) 54, 82, 131
MspI CCGG 6 cut(s) 179, 248, 340, 459, 555, 881
MspR9I CCNGG 4 cut(s) 20, 460, 621, 882
MvaI CCWGG 2 cut(s) 20, 621
MwoI GCNNNNNNNGC 6 cut(s) 433, 710, 750, 842, 922, 1099
NarI GGCGCC 1 cut(s) 775
NciI CCSGG 2 cut(s) 460, 882
NdeI CATATG 1 cut(s) 746
NdeII GATC 5 cut(s) 390, 526, 576, 727, 870
NheI GCTAGC 1 cut(s) 464
NlaIII CATG 7 cut(s) 42, 136, 616, 859, 895, 971, 1139
NlaIV GGNNCC 6 cut(s) 338, 618, 754, 776, 909, 934
NmeAIII GCCGAG 2 cut(s) 133, 806
NmuCI GTSAC 2 cut(s) 135, 201
NsiI ATGCAT 1 cut(s) 661
PaeR7I CTCGAG 1 cut(s) 469
PciSI GCTCTTC 2 cut(s) 200, 1092
PfeI GAWTC 2 cut(s) 99, 490
PinAI ACCGGT 1 cut(s) 178
PkrI GCNGC 2 cut(s) 354, 752
PleI GAGTC 2 cut(s) 463, 818
PluTI GGCGCC 1 cut(s) 778
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 2 cut(s) 463, 818
Ppu21I YACGTR 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 907
PsiI TTATAA 1 cut(s) 1161
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 2 cut(s) 18, 619
PspCI CACGTG 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 2 cut(s) 18, 619
PspN4I GGNNCC 6 cut(s) 338, 618, 754, 776, 909, 934
PspPI GGNCC 7 cut(s) 216, 316, 337, 501, 907, 933, 1104
PspPPI RGGWCCY 1 cut(s) 907
PspXI VCTCGAGB 1 cut(s) 469
PsrI GAACNNNNNNTAC 2 cut(s) 623, 655
RsaI GTAC 6 cut(s) 274, 324, 365, 548, 641, 959
RsaNI GTAC 6 cut(s) 273, 323, 364, 547, 640, 958
RseI CAYNNNNRTG 1 cut(s) 972
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 2 cut(s) 200, 1092
SaqAI TTAA 2 cut(s) 429, 1143
SatI GCNGC 2 cut(s) 353, 751
Sau3AI GATC 5 cut(s) 390, 526, 576, 727, 870
Sau96I GGNCC 7 cut(s) 216, 316, 337, 501, 907, 933, 1104
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 2 cut(s) 464, 818
ScrFI CCNGG 4 cut(s) 20, 460, 621, 882
SduI GDGCHC 6 cut(s) 195, 331, 438, 476, 927, 1054
SfaNI GCATC 2 cut(s) 668, 770
SfoI GGCGCC 1 cut(s) 776
Sfr274I CTCGAG 1 cut(s) 469
SgrAI CRCCGGYG 1 cut(s) 178
SinI GGWCC 4 cut(s) 316, 337, 501, 907
SlaI CTCGAG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 972
SmlI CTYRAG 2 cut(s) 469, 643
SmoI CTYRAG 2 cut(s) 469, 643
Sse9I AATT 1 cut(s) 600
SsiI CCGC 8 cut(s) 123, 517, 723, 742, 750, 851, 1102, 1133
SspDI GGCGCC 1 cut(s) 774
SspMI CTAG 5 cut(s) 116, 206, 465, 831, 1094
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 4 cut(s) 18, 458, 619, 880
TaaI ACNGT 3 cut(s) 94, 320, 1005
TaiI ACGT 2 cut(s) 52, 683
TaqI TCGA 5 cut(s) 252, 438, 470, 579, 706
TasI AATT 1 cut(s) 600
TatI WGTACW 2 cut(s) 363, 957
TauI GCSGC 1 cut(s) 753
TfiI GAWTC 2 cut(s) 99, 490
Tru1I TTAA 2 cut(s) 429, 1143
Tru9I TTAA 2 cut(s) 429, 1143
TscAI CASTG 1 cut(s) 491
TseFI GTSAC 2 cut(s) 135, 201
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 2 cut(s) 135, 201
TspDTI ATGAA 2 cut(s) 673, 1142
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 1 cut(s) 491
VneI GTGCAC 1 cut(s) 1050
VpaK11BI GGWCC 4 cut(s) 316, 337, 501, 907
XagI CCTNNNNNAGG 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 469
XspI CTAG 5 cut(s) 116, 206, 465, 831, 1094
ZrmI AGTACT 1 cut(s) 365
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.