FvH4_2g19820

Ubiquitin homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014116
Physical Location & Seq
Reverse (-)
16754753 .. 16756535
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19820.t1

Sequence Viewer

Length: 981 bp
ATGGAAAACGAAAGCATCAGCGATACGTACAGTTTGGATGTTATATATGTTTCCAATGATAGTTCTGAGGGGATTTTAAACTTCCATCAGTTACGCATAAACCCTAAAATTCCCTTGAATATATATACATGCATAGGCAAACTGATTGACCTCTCAGAACAAATGGATGTGACCTTTGAGCCCCAGAGAGGAAGGCCATCCTTCTCCATTGAAGTTGGTTACTTCGATACTGTCACAGAGATCAAAGAAAAGGTTCAGAAATACCAAGGAATTCCCATTTCTCAGCAAACCCTAGTATTAAATGGCCAAGTCCTCCACGACGAACACGACGTTGCCTACTGTCAAATCTTCCACCAATCCAAAATCCAGCTCCTTATCAACTCCGATTGTGATAAACAAACTTGCAATGTCAAGCTGGAAGACTCATCCCCGCCCAAGAAGGTCCATATCAATGTCAAGACCCCCATGTCCAAACTGCATGTCCCCATAGAAATGGACCTAAACGACACCGTGGCCAAGTTGAAGGAGAAGATCCAAGGCGTGGAGCCGGTTGCCCTCAGCCGTTTGGTGCTGCGTTGCACCGGTAACGAACTACAAGATCATCGATCTTTGAGTGAATGTGAGATTTCCGACAATGCAGAGATCGAGGTGAGTCTAAGACCGTCCCCAACAGCTGCTGCTTCAGCTGTTGCGGCAACAACGGTGTCCCCACGGTCGAAAAATAAGTTGAAGTTGATGGTACTGCCCAAATGTGGGACTAAAAAGATTTCGGTGGAGGTGAACGCATCGGATAATGTAGGAGAGCTGAGGAAGGAGCTGCAGAAGTTGCACCAGAGGCTACATTTTCATCTGCCTCAAGAGAGTTACTTCTTCATCTATAAGCAGAATGTGATGGACGATGACAAGTCCTTTAGGTGGCACCATGTCGGGCAGGGTGATACTATAGAAATTTTCAATGGGAGCATTACTGGTGGATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

327

Amino Acids

36.72

Weight (kDa)

5.96

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 64 - 127 8.7e-14 Ubiquitin family
ubiquitin PF00240 150 - 221 1.1e-14 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05230 AT4G05240 AT4G05260
fragaria_vesca FvH4_2g19820 FvH4_3g02641
malus_domestica MD05G1341200.v1.1 MD05G1341300.v1.1 MD10G1314800.v1.1
prunus_persica Prupe.4G026000_v2.0.a1
pyrus_communis pycom10g26690
rosa_chinensis RchiOBHm_Chr5g0003951
rosa_multiflora Rmu_sc0000547.1_g000007
rosa_roxburghii Rroxscaffold_1G00072020
rosa_samantha Rh5BG035000 Rh5CG038600 Rh5DG034600
rosa_wichuraiana Rw5G003290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 466
AccB1I GGYRCC 1 cut(s) 918
AccB7I CCANNNNNTGG 1 cut(s) 541
AciI CCGC 2 cut(s) 431, 692
AclWI GGATC 2 cut(s) 526, 969
AcoI YGGCCR 2 cut(s) 304, 513
AcsI RAATTY 3 cut(s) 108, 270, 948
AcuI CTGAAG 1 cut(s) 666
AfaI GTAC 2 cut(s) 29, 741
AfiI CCNNNNNNNGG 5 cut(s) 188, 541, 752, 753, 915
AgeI ACCGGT 1 cut(s) 581
AgsI TTSAA 5 cut(s) 118, 212, 523, 730, 955
AluBI AGCT 6 cut(s) 370, 415, 674, 686, 805, 817
AluI AGCT 6 cut(s) 370, 415, 674, 686, 805, 817
AlwI GGATC 2 cut(s) 526, 969
AlwNI CAGNNNCTG 1 cut(s) 677
AoxI GGCC 3 cut(s) 194, 304, 513
ApeKI GCWGC 4 cut(s) 571, 674, 677, 817
ApoI RAATTY 3 cut(s) 108, 270, 948
ArsI GACNNNNNNTTYG 2 cut(s) 465, 497
AsiGI ACCGGT 1 cut(s) 581
Asp700I GAANNNNTTC 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 442, 496
AsuHPI GGTGA 3 cut(s) 661, 790, 947
AvaII GGWCC 2 cut(s) 442, 496
BalI TGGCCA 2 cut(s) 306, 515
BamHI GGATCC 1 cut(s) 974
BanI GGYRCC 1 cut(s) 918
BanII GRGCYC 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 426
BbvCI CCTCAGC 2 cut(s) 557, 806
BbvI GCAGC 4 cut(s) 558, 661, 664, 804
BccI CCATC 4 cut(s) 93, 205, 730, 886
BceAI ACGGC 1 cut(s) 546
BfaI CTAG 2 cut(s) 293, 979
BfmI CTRYAG 2 cut(s) 818, 942
BisI GCNGC 5 cut(s) 572, 675, 678, 693, 818
BlsI GCNGC 5 cut(s) 573, 676, 679, 694, 819
Bme18I GGWCC 2 cut(s) 442, 496
BmgT120I GGNCC 2 cut(s) 442, 496
BmiI GGNNCC 3 cut(s) 546, 920, 976
BmsI GCATC 2 cut(s) 24, 794
BpiI GAAGAC 1 cut(s) 426
Bpu10I CCTNAGC 2 cut(s) 557, 806
BpuEI CTTGAG 1 cut(s) 840
Bsa29I ATCGAT 1 cut(s) 604
BsaAI YACGTR 1 cut(s) 27
BsaJI CCNNGG 4 cut(s) 265, 510, 535, 710
BsaWI WCCGGW 1 cut(s) 581
Bsc4I CCNNNNNNNGG 5 cut(s) 188, 541, 752, 753, 915
Bse118I RCCGGY 2 cut(s) 547, 581
Bse1I ACTGG 1 cut(s) 973
Bse3DI GCAATG 1 cut(s) 412
BseCI ATCGAT 1 cut(s) 604
BseDI CCNNGG 4 cut(s) 265, 510, 535, 710
BseGI GGATG 4 cut(s) 43, 172, 197, 425
BseLI CCNNNNNNNGG 5 cut(s) 188, 541, 752, 753, 915
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 5 cut(s) 57, 168, 296, 571, 797
BseNI ACTGG 1 cut(s) 973
BseXI GCAGC 4 cut(s) 558, 661, 664, 804
Bsh1285I CGRYCG 1 cut(s) 716
BshFI GGCC 3 cut(s) 196, 306, 515
BshNI GGYRCC 1 cut(s) 918
BshTI ACCGGT 1 cut(s) 581
BshVI ATCGAT 1 cut(s) 604
BsiEI CGRYCG 1 cut(s) 716
BsiSI CCGG 2 cut(s) 548, 582
BslFI GGGAC 4 cut(s) 467, 649, 691, 769
BslI CCNNNNNNNGG 5 cut(s) 188, 541, 752, 753, 915
BsmFI GGGAC 4 cut(s) 467, 649, 691, 769
BsnI GGCC 3 cut(s) 196, 306, 515
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 6 cut(s) 240, 531, 598, 605, 642, 974
BspACI CCGC 2 cut(s) 431, 692
BspANI GGCC 3 cut(s) 196, 306, 515
BspCNI CTCAG 5 cut(s) 58, 167, 295, 570, 798
BspDI ATCGAT 1 cut(s) 604
BspLI GGNNCC 3 cut(s) 546, 920, 976
BspMAI CTGCAG 1 cut(s) 822
BspPI GGATC 2 cut(s) 526, 969
BspT107I GGYRCC 1 cut(s) 918
BsrDI GCAATG 1 cut(s) 412
BsrFI RCCGGY 2 cut(s) 547, 581
BsrI ACTGG 1 cut(s) 973
BssAI RCCGGY 2 cut(s) 547, 581
BssECI CCNNGG 4 cut(s) 265, 510, 535, 710
BssMI GATC 6 cut(s) 240, 531, 598, 605, 642, 974
BssT1I CCWWGG 2 cut(s) 265, 535
Bst4CI ACNGT 7 cut(s) 32, 232, 341, 511, 663, 703, 714
BstAPI GCANNNNNTGC 1 cut(s) 826
BstBAI YACGTR 1 cut(s) 27
BstDEI CTNAG 6 cut(s) 66, 154, 282, 557, 656, 806
BstDSI CCRYGG 2 cut(s) 510, 710
BstF5I GGATG 4 cut(s) 43, 172, 197, 425
BstKTI GATC 6 cut(s) 243, 534, 601, 608, 645, 977
BstMBI GATC 6 cut(s) 240, 531, 598, 605, 642, 974
BstMCI CGRYCG 1 cut(s) 716
BstMWI GCNNNNNNNGC 4 cut(s) 683, 692, 826, 835
BstNSI RCATGY 2 cut(s) 132, 482
BstSFI CTRYAG 2 cut(s) 818, 942
BstSNI TACGTA 1 cut(s) 27
BstV1I GCAGC 4 cut(s) 558, 661, 664, 804
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 2 cut(s) 531, 974
BstXI CCANNNNNNTGG 1 cut(s) 493
BstYI RGATCY 2 cut(s) 531, 974
Bsu15I ATCGAT 1 cut(s) 604
BsuRI GGCC 3 cut(s) 196, 306, 515
BsuTUI ATCGAT 1 cut(s) 604
BtgI CCRYGG 2 cut(s) 510, 710
BtsCI GGATG 4 cut(s) 43, 172, 197, 425
CaiI CAGNNNCTG 1 cut(s) 677
Cfr10I RCCGGY 2 cut(s) 547, 581
Cfr13I GGNCC 2 cut(s) 442, 496
ClaI ATCGAT 1 cut(s) 604
Csp6I GTAC 2 cut(s) 28, 740
CspAI ACCGGT 1 cut(s) 581
CviAII CATG 4 cut(s) 129, 466, 479, 923
CviQI GTAC 2 cut(s) 28, 740
DdeI CTNAG 6 cut(s) 66, 154, 282, 557, 656, 806
DpnI GATC 6 cut(s) 242, 533, 600, 607, 644, 976
DpnII GATC 6 cut(s) 240, 531, 598, 605, 642, 974
DraI TTTAAA 1 cut(s) 78
DrdI GACNNNNNNGTC 1 cut(s) 466
DseDI GACNNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 2 cut(s) 304, 513
Eco105I TACGTA 1 cut(s) 27
Eco130I CCWWGG 2 cut(s) 265, 535
Eco24I GRGCYC 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 442, 496
Eco57I CTGAAG 1 cut(s) 666
EcoRI GAATTC 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 265, 535
EcoT22I ATGCAT 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 183
ErhI CCWWGG 2 cut(s) 265, 535
FaeI CATG 4 cut(s) 132, 469, 482, 926
FaqI GGGAC 4 cut(s) 467, 649, 691, 769
FatI CATG 4 cut(s) 128, 465, 478, 922
FauI CCCGC 1 cut(s) 438
Fnu4HI GCNGC 5 cut(s) 572, 675, 678, 693, 818
FokI GGATG 4 cut(s) 50, 179, 184, 412
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 5 cut(s) 572, 675, 678, 693, 818
FspBI CTAG 2 cut(s) 293, 979
GluI GCNGC 5 cut(s) 572, 675, 678, 693, 818
HaeIII GGCC 3 cut(s) 196, 306, 515
HapII CCGG 2 cut(s) 548, 582
Hin1II CATG 4 cut(s) 132, 469, 482, 926
HinfI GANTC 2 cut(s) 422, 652
HpaII CCGG 2 cut(s) 548, 582
HphI GGTGA 3 cut(s) 661, 790, 947
Hpy166II GTNNAC 1 cut(s) 781
Hpy188I TCNGA 6 cut(s) 67, 157, 258, 385, 631, 790
Hpy188III TCNNGA 2 cut(s) 457, 857
Hpy8I GTNNAC 1 cut(s) 781
Hpy99I CGWCG 2 cut(s) 323, 332
HpyAV CCTTC 5 cut(s) 186, 211, 433, 517, 805
HpyCH4III ACNGT 7 cut(s) 32, 232, 341, 511, 663, 703, 714
HpyCH4IV ACGT 2 cut(s) 26, 330
HpyCH4V TGCA 7 cut(s) 132, 405, 478, 579, 638, 820, 829
HpyF10VI GCNNNNNNNGC 4 cut(s) 683, 692, 826, 835
HpyF3I CTNAG 6 cut(s) 66, 154, 282, 557, 656, 806
HpySE526I ACGT 2 cut(s) 26, 330
Hsp92II CATG 4 cut(s) 132, 469, 482, 926
Kzo9I GATC 6 cut(s) 240, 531, 598, 605, 642, 974
LmnI GCTCC 4 cut(s) 375, 544, 814, 960
LpnPI CCDG 8 cut(s) 197, 380, 401, 561, 595, 845, 917, 954
Lsp1109I GCAGC 4 cut(s) 558, 661, 664, 804
LweI GCATC 2 cut(s) 24, 794
MaeI CTAG 2 cut(s) 293, 979
MaeII ACGT 2 cut(s) 26, 330
MaeIII GTNAC 6 cut(s) 90, 169, 218, 232, 584, 863
MalI GATC 6 cut(s) 242, 533, 600, 607, 644, 976
MboI GATC 6 cut(s) 240, 531, 598, 605, 642, 974
MboII GAAGA 4 cut(s) 340, 431, 541, 862
MflI RGATCY 2 cut(s) 531, 974
MhlI GDGCHC 1 cut(s) 183
MlsI TGGCCA 2 cut(s) 306, 515
MluCI AATT 3 cut(s) 108, 270, 948
MluNI TGGCCA 2 cut(s) 306, 515
MlyI GAGTC 2 cut(s) 416, 661
MmeI TCCRAC 1 cut(s) 654
Mox20I TGGCCA 2 cut(s) 306, 515
Mph1103I ATGCAT 1 cut(s) 134
MroXI GAANNNNTTC 1 cut(s) 252
MscI TGGCCA 2 cut(s) 306, 515
MseI TTAA 2 cut(s) 77, 299
MslI CAYNNNNRTG 2 cut(s) 450, 491
Msp20I TGGCCA 2 cut(s) 306, 515
MspA1I CMGCKG 2 cut(s) 674, 686
MspI CCGG 2 cut(s) 548, 582
MwoI GCNNNNNNNGC 4 cut(s) 683, 692, 826, 835
NdeII GATC 6 cut(s) 240, 531, 598, 605, 642, 974
NlaIII CATG 4 cut(s) 132, 469, 482, 926
NlaIV GGNNCC 3 cut(s) 546, 920, 976
NmuCI GTSAC 2 cut(s) 169, 232
NsiI ATGCAT 1 cut(s) 134
NspI RCATGY 2 cut(s) 132, 482
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 327
PdmI GAANNNNTTC 1 cut(s) 252
PflMI CCANNNNNTGG 1 cut(s) 541
PinAI ACCGGT 1 cut(s) 581
PkrI GCNGC 5 cut(s) 573, 676, 679, 694, 819
PleI GAGTC 2 cut(s) 416, 660
PpsI GAGTC 2 cut(s) 416, 660
Ppu21I YACGTR 1 cut(s) 27
PspN4I GGNNCC 3 cut(s) 546, 920, 976
PspPI GGNCC 2 cut(s) 442, 496
PstI CTGCAG 1 cut(s) 822
PstNI CAGNNNCTG 1 cut(s) 677
PsuI RGATCY 2 cut(s) 531, 974
PvuII CAGCTG 2 cut(s) 674, 686
RsaI GTAC 2 cut(s) 29, 741
RsaNI GTAC 2 cut(s) 28, 740
RseI CAYNNNNRTG 2 cut(s) 450, 491
SaqAI TTAA 2 cut(s) 77, 299
SatI GCNGC 5 cut(s) 572, 675, 678, 693, 818
Sau3AI GATC 6 cut(s) 240, 531, 598, 605, 642, 974
Sau96I GGNCC 2 cut(s) 442, 496
SchI GAGTC 2 cut(s) 416, 661
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 2 cut(s) 24, 794
SfcI CTRYAG 2 cut(s) 818, 942
SinI GGWCC 2 cut(s) 442, 496
SmiMI CAYNNNNRTG 2 cut(s) 450, 491
SmlI CTYRAG 1 cut(s) 855
SmoI CTYRAG 1 cut(s) 855
SnaBI TACGTA 1 cut(s) 27
Sse9I AATT 3 cut(s) 108, 270, 948
SsiI CCGC 2 cut(s) 431, 692
SspMI CTAG 2 cut(s) 293, 979
StyI CCWWGG 2 cut(s) 265, 535
TaaI ACNGT 7 cut(s) 32, 232, 341, 511, 663, 703, 714
TaiI ACGT 2 cut(s) 29, 333
TaqI TCGA 4 cut(s) 225, 604, 645, 716
TasI AATT 3 cut(s) 108, 270, 948
TauI GCSGC 1 cut(s) 695
Tru1I TTAA 2 cut(s) 77, 299
Tru9I TTAA 2 cut(s) 77, 299
TseFI GTSAC 2 cut(s) 169, 232
TseI GCWGC 4 cut(s) 571, 674, 677, 817
Tsp45I GTSAC 2 cut(s) 169, 232
TspDTI ATGAA 2 cut(s) 836, 862
Van91I CCANNNNNTGG 1 cut(s) 541
VpaK11BI GGWCC 2 cut(s) 442, 496
XapI RAATTY 3 cut(s) 108, 270, 948
XceI RCATGY 2 cut(s) 132, 482
XmnI GAANNNNTTC 1 cut(s) 252
XspI CTAG 2 cut(s) 293, 979
Zsp2I ATGCAT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.