MD10G1314800.v1.1

Ubiquitin homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014116
Physical Location & Seq
Forward (+)
39891033 .. 39891899
867 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1314800.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGATGTTATTTTTGAGCTCCAGAGAGGAAGATCCTTCACAATTGAAGTTGGTTATTTCGACACCGTTCTCGAGATCAAAGAGAAGGTTCAGAAATACCAAGGAATTCCCATTTCCCACCAAACCTTAATCTTCAACGGCCGAGTTCTCGATGACGAACGCAACATTGAATACTGCGACATCTACAACAATTCCCGCATCCAACTCCTCGTCGCATCTGAATTCGATAAACCGGTGAACAACAGTAATTGTAATAACAATGTGAAGGCTATGGAGGAGTACTGCCCTAAGAAAATCCAGCTCAACGTGAAGACCCCAACGTCCAAAATGCATGTCCCTATTGAAATGGACGTAAACGACACTGTCTCGAAGCTAAAAGAGAATATCCAAGCGATGGATCAGTCCATTCCACTTAATCGTTTGGTGGTTCATGCAGTTGGGAATGAGTTACATGATCATCGCTCGTTGCTCGAATGCGAGCTTTCGGACAATGCGGAGATAGAGGTGAGCTTGAGACCGTCACCAGCTCCTGCAACCAATGCGAGCTCACCCGTGTCAATGGGTGGGGCTGGAGTTGGCGGCGGAGGCGGAATGGGGTCCAAAAATAAGCTGAAGCTTATGGTCTTGACAAAGTGTGGGACGAAGAAGATTGCGGTGGAAGTGAACCCATTGGATAATGTAGGGGAATTGAGGAAGGAGCTGCAGAGGTTGCACCAAAGGCAAATGAACTTTCATCTGCCGCAAGAGAGCTATTTCTTCATCTATAAGCAGAATGTGATGGAGGACGACAGGTCGTTTCGATGGCATCAAGTTGCGCAGGGTGATACTATAGAGATTTTTAATGGTAGCGTAACCGGTGGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

289

Amino Acids

32.38

Weight (kDa)

5.94

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 6 - 71 2.9e-18 Ubiquitin family
ubiquitin PF00240 101 - 173 3.3e-09 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05230 AT4G05240 AT4G05260
fragaria_vesca FvH4_2g19820 FvH4_3g02641
malus_domestica MD05G1341200.v1.1 MD05G1341300.v1.1 MD10G1314800.v1.1
prunus_persica Prupe.4G026000_v2.0.a1
pyrus_communis pycom10g26690
rosa_chinensis RchiOBHm_Chr5g0003951
rosa_multiflora Rmu_sc0000547.1_g000007
rosa_roxburghii Rroxscaffold_1G00072020
rosa_samantha Rh5BG035000 Rh5CG038600 Rh5DG034600
rosa_wichuraiana Rw5G003290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
Acc16I TGCGCA 1 cut(s) 816
AccB7I CCANNNNNTGG 1 cut(s) 394
AciI CCGC 7 cut(s) 196, 494, 579, 582, 588, 653, 740
AclWI GGATC 2 cut(s) 27, 405
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 2 cut(s) 105, 221
AcuI CTGAAG 1 cut(s) 632
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 394
AgeI ACCGGT 2 cut(s) 232, 854
AgsI TTSAA 4 cut(s) 47, 136, 170, 344
AhdI GACNNNNNGTC 1 cut(s) 790
Alw21I GWGCWC 2 cut(s) 21, 548
Alw26I GTCTC 2 cut(s) 370, 508
AlwI GGATC 2 cut(s) 27, 405
AlwNI CAGNNNCTG 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 71
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 700
ApoI RAATTY 2 cut(s) 105, 221
ArsI GACNNNNNNTTYG 2 cut(s) 318, 350
AsiGI ACCGGT 2 cut(s) 232, 854
AspLEI GCGC 1 cut(s) 817
AspS9I GGNCC 1 cut(s) 597
AsuHPI GGTGA 5 cut(s) 247, 513, 517, 540, 833
AvaI CYCGRG 1 cut(s) 71
AvaII GGWCC 1 cut(s) 597
BanII GRGCYC 2 cut(s) 21, 548
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 2 cut(s) 21, 548
BbvI GCAGC 1 cut(s) 687
BccI CCATC 3 cut(s) 388, 772, 795
BceAI ACGGC 1 cut(s) 154
BclI TGATCA 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 370, 508
BfmI CTRYAG 2 cut(s) 701, 828
BisI GCNGC 3 cut(s) 580, 701, 740
BlsI GCNGC 3 cut(s) 581, 702, 741
BmcAI AGTACT 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 597
BmeRI GACNNNNNGTC 1 cut(s) 790
BmeT110I CYCGRG 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 597
BmiI GGNNCC 1 cut(s) 598
BmsI GCATC 3 cut(s) 207, 224, 814
BpiI GAAGAC 1 cut(s) 317
BpmI CTGGAG 2 cut(s) 5, 591
BpuEI CTTGAG 1 cut(s) 532
BsaI GGTCTC 1 cut(s) 508
BsaJI CCNNGG 1 cut(s) 100
BsaWI WCCGGW 2 cut(s) 232, 854
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse118I RCCGGY 2 cut(s) 232, 854
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 2 cut(s) 10, 198
BseLI CCNNNNNNNGG 1 cut(s) 394
BseRI GAGGAG 2 cut(s) 197, 290
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 1 cut(s) 687
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 1 cut(s) 141
BshTI ACCGGT 2 cut(s) 232, 854
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 2 cut(s) 21, 548
BsiHKCI CYCGRG 1 cut(s) 71
BsiSI CCGG 2 cut(s) 233, 855
BslFI GGGAC 2 cut(s) 320, 652
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 2 cut(s) 370, 508
BsmFI GGGAC 2 cut(s) 320, 652
BsmI GAATGC 1 cut(s) 479
BsnI GGCC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 508
BsoBI CYCGRG 1 cut(s) 71
Bsp1286I GDGCHC 2 cut(s) 21, 548
Bsp143I GATC 5 cut(s) 32, 75, 397, 454, 860
BspACI CCGC 7 cut(s) 196, 494, 579, 582, 588, 653, 740
BspANI GGCC 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 598
BspMAI CTGCAG 1 cut(s) 705
BspPI GGATC 2 cut(s) 27, 405
BspTNI GGTCTC 1 cut(s) 508
BsrFI RCCGGY 2 cut(s) 232, 854
BssAI RCCGGY 2 cut(s) 232, 854
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 5 cut(s) 32, 75, 397, 454, 860
BssT1I CCWWGG 1 cut(s) 100
Bst4CI ACNGT 4 cut(s) 67, 245, 364, 519
BstAPI GCANNNNNTGC 2 cut(s) 539, 709
BstC8I GCNNGC 2 cut(s) 479, 544
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 10, 198
BstHHI GCGC 1 cut(s) 817
BstKTI GATC 5 cut(s) 35, 78, 400, 457, 863
BstMAI GTCTC 2 cut(s) 370, 508
BstMBI GATC 5 cut(s) 32, 75, 397, 454, 860
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 4 cut(s) 539, 585, 709, 718
BstNSI RCATGY 1 cut(s) 335
BstSFI CTRYAG 2 cut(s) 701, 828
BstV1I GCAGC 1 cut(s) 687
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BstZI CGGCCG 1 cut(s) 139
BsuRI GGCC 1 cut(s) 141
BtgZI GCGATG 2 cut(s) 407, 443
BtsCI GGATG 2 cut(s) 10, 198
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 2 cut(s) 479, 544
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 1 cut(s) 817
Cfr10I RCCGGY 2 cut(s) 232, 854
Cfr13I GGNCC 1 cut(s) 597
Csp6I GTAC 1 cut(s) 280
CspAI ACCGGT 2 cut(s) 232, 854
CviAII CATG 3 cut(s) 332, 431, 452
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 1 cut(s) 288
DpnI GATC 5 cut(s) 34, 77, 399, 456, 862
DpnII GATC 5 cut(s) 32, 75, 397, 454, 860
DrdI GACNNNNNNGTC 1 cut(s) 319
DriI GACNNNNNGTC 1 cut(s) 790
DseDI GACNNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 139
EagI CGGCCG 1 cut(s) 139
Eam1105I GACNNNNNGTC 1 cut(s) 790
EciI GGCGGA 2 cut(s) 597, 603
Ecl136II GAGCTC 2 cut(s) 19, 546
EclXI CGGCCG 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 2 cut(s) 21, 548
Eco31I GGTCTC 1 cut(s) 508
Eco47I GGWCC 1 cut(s) 597
Eco52I CGGCCG 1 cut(s) 139
Eco53kI GAGCTC 2 cut(s) 19, 546
Eco57I CTGAAG 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 71
EcoICRI GAGCTC 2 cut(s) 19, 546
EcoRI GAATTC 2 cut(s) 105, 221
EcoT14I CCWWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 333
EcoT38I GRGCYC 2 cut(s) 21, 548
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 3 cut(s) 335, 434, 455
FaiI YATR 8 cut(s) 272, 333, 432, 453, 620, 765, 830, 865
FaqI GGGAC 2 cut(s) 320, 652
FatI CATG 3 cut(s) 331, 430, 451
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 3 cut(s) 580, 701, 740
FokI GGATG 2 cut(s) 17, 185
FriOI GRGCYC 2 cut(s) 21, 548
Fsp4HI GCNGC 3 cut(s) 580, 701, 740
FspI TGCGCA 1 cut(s) 816
GlaI GCGC 1 cut(s) 816
GluI GCNGC 3 cut(s) 580, 701, 740
GsuI CTGGAG 2 cut(s) 5, 591
HaeIII GGCC 1 cut(s) 141
HapII CCGG 2 cut(s) 233, 855
HhaI GCGC 1 cut(s) 817
Hin1II CATG 3 cut(s) 335, 434, 455
Hin6I GCGC 1 cut(s) 815
HinP1I GCGC 1 cut(s) 815
HindIII AAGCTT 1 cut(s) 614
HpaII CCGG 2 cut(s) 233, 855
HphI GGTGA 5 cut(s) 247, 513, 517, 540, 833
Hpy166II GTNNAC 3 cut(s) 238, 355, 664
Hpy188I TCNGA 3 cut(s) 93, 220, 487
Hpy188III TCNNGA 6 cut(s) 22, 71, 73, 149, 367, 625
Hpy8I GTNNAC 3 cut(s) 238, 355, 664
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 4 cut(s) 46, 79, 259, 688
HpyCH4III ACNGT 4 cut(s) 67, 245, 364, 519
HpyCH4IV ACGT 3 cut(s) 306, 320, 351
HpyCH4V TGCA 5 cut(s) 331, 434, 533, 703, 712
HpyF10VI GCNNNNNNNGC 4 cut(s) 539, 585, 709, 718
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 3 cut(s) 306, 320, 351
Hsp92II CATG 3 cut(s) 335, 434, 455
HspAI GCGC 1 cut(s) 815
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 5 cut(s) 32, 75, 397, 454, 860
LmnI GCTCC 3 cut(s) 24, 532, 697
LpnPI CCDG 8 cut(s) 35, 246, 311, 537, 543, 555, 775, 803
Lsp1109I GCAGC 1 cut(s) 687
LweI GCATC 3 cut(s) 207, 224, 814
MaeII ACGT 3 cut(s) 306, 320, 351
MaeIII GTNAC 3 cut(s) 447, 519, 850
MalI GATC 5 cut(s) 34, 77, 399, 456, 862
MboI GATC 5 cut(s) 32, 75, 397, 454, 860
MboII GAAGA 6 cut(s) 42, 124, 322, 655, 658, 748
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 21, 548
MluCI AATT 6 cut(s) 42, 105, 190, 221, 247, 686
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 8 cut(s) 20, 218, 268, 496, 578, 684, 699, 775
Mph1103I ATGCAT 1 cut(s) 333
MseI TTAA 3 cut(s) 128, 414, 840
MspI CCGG 2 cut(s) 233, 855
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 479
MwoI GCNNNNNNNGC 4 cut(s) 539, 585, 709, 718
NdeII GATC 5 cut(s) 32, 75, 397, 454, 860
NlaIII CATG 3 cut(s) 335, 434, 455
NlaIV GGNNCC 1 cut(s) 598
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 1 cut(s) 519
NsbI TGCGCA 1 cut(s) 816
NsiI ATGCAT 1 cut(s) 333
NspI RCATGY 1 cut(s) 335
PaeR7I CTCGAG 1 cut(s) 71
PctI GAATGC 1 cut(s) 479
PflFI GACNNNGTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 394
PinAI ACCGGT 2 cut(s) 232, 854
PkrI GCNGC 3 cut(s) 581, 702, 741
Psp124BI GAGCTC 2 cut(s) 21, 548
PspN4I GGNNCC 1 cut(s) 598
PspPI GGNCC 1 cut(s) 597
PstI CTGCAG 1 cut(s) 705
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 32
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SacI GAGCTC 2 cut(s) 21, 548
SaqAI TTAA 3 cut(s) 128, 414, 840
SatI GCNGC 3 cut(s) 580, 701, 740
Sau3AI GATC 5 cut(s) 32, 75, 397, 454, 860
Sau96I GGNCC 1 cut(s) 597
ScaI AGTACT 1 cut(s) 281
SduI GDGCHC 2 cut(s) 21, 548
SfaNI GCATC 3 cut(s) 207, 224, 814
SfcI CTRYAG 2 cut(s) 701, 828
Sfr274I CTCGAG 1 cut(s) 71
SinI GGWCC 1 cut(s) 597
SlaI CTCGAG 1 cut(s) 71
SmlI CTYRAG 2 cut(s) 71, 511
SmoI CTYRAG 2 cut(s) 71, 511
Sse9I AATT 6 cut(s) 42, 105, 190, 221, 247, 686
SsiI CCGC 7 cut(s) 196, 494, 579, 582, 588, 653, 740
SstI GAGCTC 2 cut(s) 21, 548
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 4 cut(s) 67, 245, 364, 519
TaiI ACGT 3 cut(s) 309, 323, 354
TaqI TCGA 7 cut(s) 60, 72, 150, 225, 368, 471, 799
TasI AATT 6 cut(s) 42, 105, 190, 221, 247, 686
TatI WGTACW 1 cut(s) 279
TauI GCSGC 2 cut(s) 582, 742
Tru1I TTAA 3 cut(s) 128, 414, 840
Tru9I TTAA 3 cut(s) 128, 414, 840
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 1 cut(s) 700
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 4 cut(s) 419, 722, 740, 748
TspRI CASTG 1 cut(s) 367
Tth111I GACNNNGTC 1 cut(s) 362
Van91I CCANNNNNTGG 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 597
XapI RAATTY 2 cut(s) 105, 221
XceI RCATGY 1 cut(s) 335
XhoI CTCGAG 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 281
Zsp2I ATGCAT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.