Prupe.4G026000_v2.0.a1

Ubiquitin homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014116
Physical Location & Seq
Reverse (-)
1218049 .. 1218906
858 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G026000.1

Sequence Viewer

Length: 858 bp
ATGGATGTGATTTTTGAGCCCCAAAGAGGAAGATCCTTCACCATTGAAGTGGGTTACTTTGACACAGTCCTGGAGATCAAAGAAAAGGTCCAGAAATACCAGGGCATTTCTGTTCCCCACCAAACCCTAGTCTTCAATGGCCAAGTCCTTGAAGACGACAAAGACGTTGCCTACTGTGAGATCCTCAACAACTCTCACATTCAACTCATCGTTGCCTCTGAATCCGAAAAACCCACCACCACCAACAACAACAACAATGTCAAGGTGGAAGACTCGCCGGCGCCGTCCAAGAAAATCCAGCTCAACGTGAAGACCCCCACGTCCAAAATGCATGTCCCTGTTGAAATGGACATAAACGACACCGTTACAAAACTAAAAGAAAAAATTCAAGCGATGGATCAAGCTGTCCCAATTGGTCGCTTGGTGCTCCATTCACTTGGCAATGAGTTGCAAGATCATCGTTCCTTACGTGATTGTGAGCTTTCAGACAATGCGGAGATCGAGGTGAGCTTGAGACCGTCCCCAACGGCAGCGGCAGCGGTGGCTTCACCGCTGGCAATGGGTGGTCCCGGGGCCGGGGGTGTGGCGGTGTCGAGGAATAAGTTGAAGCTGATGGTGTTGCCCAAGTGCGGGACGAAGAAAATTCCGGTGGAAATGAACCCATCAGATAATGTAGGGGAGCTAAGAAAGGAGCTTCAGAAGTTGCACCACAGGCTACACTTTCATTTGCCACAAGAGAGCTATTTCTTCATCTATAAGCAGAATGTGATGGAGGACGACAGGTCGTTTCGGTGGCATCAAGTTGGGCAGGGTGATACTATAGAGATTTTCAATGGGAGCGTTACCGGTGGATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

286

Amino Acids

31.72

Weight (kDa)

6.07

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05230 AT4G05240 AT4G05260
fragaria_vesca FvH4_2g19820 FvH4_3g02641
malus_domestica MD05G1341200.v1.1 MD05G1341300.v1.1 MD10G1314800.v1.1
prunus_persica Prupe.4G026000_v2.0.a1
pyrus_communis pycom10g26690
rosa_chinensis RchiOBHm_Chr5g0003951
rosa_multiflora Rmu_sc0000547.1_g000007
rosa_roxburghii Rroxscaffold_1G00072020
rosa_samantha Rh5BG035000 Rh5CG038600 Rh5DG034600
rosa_wichuraiana Rw5G003290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
AccB1I GGYRCC 1 cut(s) 280
AciI CCGC 6 cut(s) 494, 533, 539, 551, 587, 630
AclWI GGATC 4 cut(s) 27, 175, 405, 846
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 2 cut(s) 384, 642
AcuI CTGAAG 1 cut(s) 680
AcyI GRCGYC 1 cut(s) 281
AfiI CCNNNNNNNGG 5 cut(s) 26, 575, 576, 629, 630
AgeI ACCGGT 1 cut(s) 845
AgsI TTSAA 8 cut(s) 47, 136, 152, 203, 344, 389, 607, 832
AhdI GACNNNNNGTC 1 cut(s) 781
AjiI CACGTC 1 cut(s) 321
AjnI CCWGG 2 cut(s) 69, 99
AluBI AGCT 8 cut(s) 301, 404, 481, 510, 610, 682, 694, 741
AluI AGCT 8 cut(s) 301, 404, 481, 510, 610, 682, 694, 741
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 508
AlwI GGATC 4 cut(s) 27, 175, 405, 846
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 2 cut(s) 139, 573
ApeKI GCWGC 2 cut(s) 530, 536
ApoI RAATTY 2 cut(s) 384, 642
ArsI GACNNNNNNTTYG 4 cut(s) 72, 104, 318, 350
AsiGI ACCGGT 1 cut(s) 845
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 3 cut(s) 88, 566, 573
AsuC2I CCSGG 3 cut(s) 570, 571, 577
AsuHPI GGTGA 4 cut(s) 31, 517, 540, 824
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 2 cut(s) 88, 566
BalI TGGCCA 1 cut(s) 141
BamHI GGATCC 1 cut(s) 851
BanI GGYRCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 4 cut(s) 124, 159, 276, 317
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 542, 548
BccI CCATC 4 cut(s) 388, 607, 670, 763
BceAI ACGGC 2 cut(s) 268, 543
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 2 cut(s) 71, 101
BcnI CCSGG 3 cut(s) 570, 571, 577
BcoDI GTCTC 1 cut(s) 508
BfaI CTAG 2 cut(s) 128, 856
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 284
BisI GCNGC 3 cut(s) 531, 534, 537
BlsI GCNGC 3 cut(s) 532, 535, 538
Bme1390I CCNGG 5 cut(s) 71, 101, 570, 571, 577
Bme18I GGWCC 2 cut(s) 88, 566
BmeRI GACNNNNNGTC 1 cut(s) 781
BmeT110I CYCGRG 1 cut(s) 569
BmgBI CACGTC 1 cut(s) 321
BmgT120I GGNCC 3 cut(s) 88, 566, 573
BmiI GGNNCC 4 cut(s) 282, 568, 574, 853
BmrFI CCNGG 5 cut(s) 71, 101, 570, 571, 577
BmsI GCATC 1 cut(s) 805
BpiI GAAGAC 4 cut(s) 124, 159, 276, 317
BpmI CTGGAG 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 532
BpuMI CCSGG 3 cut(s) 570, 571, 577
BsaAI YACGTR 1 cut(s) 470
BsaHI GRCGYC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 508
BsaJI CCNNGG 4 cut(s) 100, 569, 570, 576
BsaWI WCCGGW 2 cut(s) 646, 845
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 575, 576, 629, 630
Bse118I RCCGGY 2 cut(s) 277, 845
Bse3DI GCAATG 2 cut(s) 448, 564
BseBI CCWGG 2 cut(s) 71, 101
BseDI CCNNGG 4 cut(s) 100, 569, 570, 576
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 5 cut(s) 26, 575, 576, 629, 630
BseMI GCAATG 2 cut(s) 448, 564
BseXI GCAGC 2 cut(s) 542, 548
BshFI GGCC 2 cut(s) 141, 575
BshNI GGYRCC 1 cut(s) 280
BshTI ACCGGT 1 cut(s) 845
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 5 cut(s) 278, 570, 576, 647, 846
BslFI GGGAC 5 cut(s) 320, 392, 505, 552, 646
BslI CCNNNNNNNGG 5 cut(s) 26, 575, 576, 629, 630
BsmAI GTCTC 1 cut(s) 508
BsmFI GGGAC 5 cut(s) 320, 392, 505, 552, 646
BsnI GGCC 2 cut(s) 141, 575
Bso31I GGTCTC 1 cut(s) 508
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 21, 429
Bsp143I GATC 7 cut(s) 32, 75, 180, 397, 454, 498, 851
BspACI CCGC 6 cut(s) 494, 533, 539, 551, 587, 630
BspANI GGCC 2 cut(s) 141, 575
BspLI GGNNCC 4 cut(s) 282, 568, 574, 853
BspPI GGATC 4 cut(s) 27, 175, 405, 846
BspT107I GGYRCC 1 cut(s) 280
BspTNI GGTCTC 1 cut(s) 508
BsrDI GCAATG 2 cut(s) 448, 564
BsrFI RCCGGY 2 cut(s) 277, 845
BssAI RCCGGY 2 cut(s) 277, 845
BssECI CCNNGG 4 cut(s) 100, 569, 570, 576
BssMI GATC 7 cut(s) 32, 75, 180, 397, 454, 498, 851
BssNI GRCGYC 1 cut(s) 281
Bst2UI CCWGG 2 cut(s) 71, 101
Bst4CI ACNGT 4 cut(s) 67, 176, 364, 519
BstACI GRCGYC 1 cut(s) 281
BstBAI YACGTR 1 cut(s) 470
BstC8I GCNNGC 2 cut(s) 279, 555
BstDEI CTNAG 1 cut(s) 683
BstF5I GGATG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 7 cut(s) 35, 78, 183, 400, 457, 501, 854
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 7 cut(s) 32, 75, 180, 397, 454, 498, 851
BstMWI GCNNNNNNNGC 3 cut(s) 536, 542, 712
BstNI CCWGG 2 cut(s) 71, 101
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 5 cut(s) 69, 99, 568, 569, 575
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 2 cut(s) 542, 548
BstV2I GAAGAC 4 cut(s) 124, 159, 276, 317
BstX2I RGATCY 3 cut(s) 32, 180, 851
BstXI CCANNNNNNTGG 2 cut(s) 49, 437
BstYI RGATCY 3 cut(s) 32, 180, 851
BsuRI GGCC 2 cut(s) 141, 575
BtgZI GCGATG 1 cut(s) 407
BtrI CACGTC 1 cut(s) 321
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 2 cut(s) 279, 555
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 2 cut(s) 277, 845
Cfr13I GGNCC 3 cut(s) 88, 566, 573
Cfr9I CCCGGG 1 cut(s) 569
CspAI ACCGGT 1 cut(s) 845
CviAII CATG 1 cut(s) 332
DdeI CTNAG 1 cut(s) 683
DinI GGCGCC 1 cut(s) 282
DpnI GATC 7 cut(s) 34, 77, 182, 399, 456, 500, 853
DpnII GATC 7 cut(s) 32, 75, 180, 397, 454, 498, 851
DrdI GACNNNNNNGTC 1 cut(s) 319
DriI GACNNNNNGTC 1 cut(s) 781
DseDI GACNNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 139
Eam1105I GACNNNNNGTC 1 cut(s) 781
Eco24I GRGCYC 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 508
Eco47I GGWCC 2 cut(s) 88, 566
Eco57I CTGAAG 1 cut(s) 680
Eco88I CYCGRG 1 cut(s) 569
EcoRII CCWGG 2 cut(s) 69, 99
EcoT22I ATGCAT 1 cut(s) 333
EcoT38I GRGCYC 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
FaeI CATG 1 cut(s) 335
FaiI YATR 4 cut(s) 333, 353, 756, 821
FaqI GGGAC 5 cut(s) 320, 392, 505, 552, 646
FatI CATG 1 cut(s) 331
FauI CCCGC 1 cut(s) 623
Fnu4HI GCNGC 3 cut(s) 531, 534, 537
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 3 cut(s) 531, 534, 537
FspBI CTAG 2 cut(s) 128, 856
GlaI GCGC 1 cut(s) 282
GluI GCNGC 3 cut(s) 531, 534, 537
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 2 cut(s) 141, 575
HapII CCGG 5 cut(s) 278, 570, 576, 647, 846
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 1 cut(s) 335
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 2 cut(s) 221, 272
HpaII CCGG 5 cut(s) 278, 570, 576, 647, 846
HphI GGTGA 4 cut(s) 31, 517, 540, 824
Hpy188I TCNGA 5 cut(s) 220, 226, 487, 667, 699
Hpy188III TCNNGA 1 cut(s) 91
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 4 cut(s) 67, 176, 364, 519
HpyCH4IV ACGT 4 cut(s) 165, 306, 320, 469
HpyCH4V TGCA 3 cut(s) 331, 451, 706
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 542, 712
HpyF3I CTNAG 1 cut(s) 683
HpySE526I ACGT 4 cut(s) 165, 306, 320, 469
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 1 cut(s) 335
HspAI GCGC 1 cut(s) 281
KasI GGCGCC 1 cut(s) 280
KroI GCCGGC 1 cut(s) 277
KroNI GCCGGC 1 cut(s) 279
Kzo9I GATC 7 cut(s) 32, 75, 180, 397, 454, 498, 851
LmnI GCTCC 4 cut(s) 432, 679, 691, 837
Lsp1109I GCAGC 2 cut(s) 542, 548
LweI GCATC 1 cut(s) 805
MaeI CTAG 2 cut(s) 128, 856
MaeII ACGT 4 cut(s) 165, 306, 320, 469
MaeIII GTNAC 3 cut(s) 53, 364, 841
MalI GATC 7 cut(s) 34, 77, 182, 399, 456, 500, 853
MboI GATC 7 cut(s) 32, 75, 180, 397, 454, 498, 851
MboII GAAGA 7 cut(s) 42, 124, 164, 281, 322, 649, 739
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 3 cut(s) 32, 180, 851
MhlI GDGCHC 2 cut(s) 21, 429
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 3 cut(s) 384, 411, 642
MluNI TGGCCA 1 cut(s) 141
Mly113I GGCGCC 1 cut(s) 281
MlyI GAGTC 1 cut(s) 266
MnlI CCTC 6 cut(s) 20, 194, 226, 496, 588, 766
Mox20I TGGCCA 1 cut(s) 141
Mph1103I ATGCAT 1 cut(s) 333
MreI CGCCGGCG 1 cut(s) 277
MroNI GCCGGC 1 cut(s) 277
MroXI GAANNNNTTC 1 cut(s) 384
MscI TGGCCA 1 cut(s) 141
MslI CAYNNNNRTG 1 cut(s) 47
Msp20I TGGCCA 1 cut(s) 141
MspA1I CMGCKG 3 cut(s) 533, 539, 553
MspI CCGG 5 cut(s) 278, 570, 576, 647, 846
MspR9I CCNGG 5 cut(s) 71, 101, 570, 571, 577
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 2 cut(s) 71, 101
MwoI GCNNNNNNNGC 3 cut(s) 536, 542, 712
NaeI GCCGGC 1 cut(s) 279
NarI GGCGCC 1 cut(s) 281
NciI CCSGG 3 cut(s) 570, 571, 577
NdeII GATC 7 cut(s) 32, 75, 180, 397, 454, 498, 851
NgoMIV GCCGGC 1 cut(s) 277
NlaIII CATG 1 cut(s) 335
NlaIV GGNNCC 4 cut(s) 282, 568, 574, 853
NsiI ATGCAT 1 cut(s) 333
NspI RCATGY 1 cut(s) 335
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 281, 466
PdiI GCCGGC 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 221
PflFI GACNNNGTC 1 cut(s) 65
PfoI TCCNGGA 1 cut(s) 69
PinAI ACCGGT 1 cut(s) 845
PkrI GCNGC 3 cut(s) 532, 535, 538
PleI GAGTC 1 cut(s) 266
PluTI GGCGCC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 266
Ppu21I YACGTR 1 cut(s) 470
Psp6I CCWGG 2 cut(s) 69, 99
PspGI CCWGG 2 cut(s) 69, 99
PspN4I GGNNCC 4 cut(s) 282, 568, 574, 853
PspPI GGNCC 3 cut(s) 88, 566, 573
PsuI RGATCY 3 cut(s) 32, 180, 851
PsyI GACNNNGTC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 47
SatI GCNGC 3 cut(s) 531, 534, 537
Sau3AI GATC 7 cut(s) 32, 75, 180, 397, 454, 498, 851
Sau96I GGNCC 3 cut(s) 88, 566, 573
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 5 cut(s) 71, 101, 570, 571, 577
SduI GDGCHC 2 cut(s) 21, 429
SfaNI GCATC 1 cut(s) 805
SfcI CTRYAG 1 cut(s) 819
SfoI GGCGCC 1 cut(s) 282
SgrAI CRCCGGYG 1 cut(s) 277
SinI GGWCC 2 cut(s) 88, 566
SmaI CCCGGG 1 cut(s) 571
SmiMI CAYNNNNRTG 1 cut(s) 47
SmlI CTYRAG 1 cut(s) 511
SmoI CTYRAG 1 cut(s) 511
Sse9I AATT 3 cut(s) 384, 411, 642
SsiI CCGC 6 cut(s) 494, 533, 539, 551, 587, 630
SspDI GGCGCC 1 cut(s) 280
SspMI CTAG 2 cut(s) 128, 856
StyD4I CCNGG 5 cut(s) 69, 99, 568, 569, 575
TaaI ACNGT 4 cut(s) 67, 176, 364, 519
TaiI ACGT 4 cut(s) 168, 309, 323, 472
TaqI TCGA 2 cut(s) 501, 593
TasI AATT 3 cut(s) 384, 411, 642
TauI GCSGC 1 cut(s) 536
TfiI GAWTC 1 cut(s) 221
TseI GCWGC 2 cut(s) 530, 536
TspDTI ATGAA 3 cut(s) 671, 713, 739
TspMI CCCGGG 1 cut(s) 569
Tth111I GACNNNGTC 1 cut(s) 65
VpaK11BI GGWCC 2 cut(s) 88, 566
XapI RAATTY 2 cut(s) 384, 642
XceI RCATGY 1 cut(s) 335
XmaI CCCGGG 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 2 cut(s) 128, 856
Zsp2I ATGCAT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.