FvH4_2g22470

Photosystem II reaction centre W protein (PsbW)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015130
Physical Location & Seq
Forward (+)
18435162 .. 18436162
1001 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22470.t1

Sequence Viewer

Length: 411 bp
ATGGCCTCCTTCACAGCTAGCACTCCCACCTCATCTGTCACCAGGGCAGCCCTTGTGCACAAGCCATCTGTTGGGGCTCCATCCTCAACTATTCTAGCTTTGCCATCAATTGGAAACAAGGGAAAGCTGAGATGTTCAATGGAGGCAAAGCCCCCAATGAAGGAGAGCAACTCAAACATTGGGATGAGTGCATCTCTGGTAGCTGCAGCTTTGGCTGCAACCATGTCAAGCCCAGCAGCCATGGCATTGGTGGATGATAGATTGAGCACAGAAGGAACAGGGCTTCCCTTTGGTTTGAGCAACAATCTTCTCGGGTGGATCCTTCTTGGTGTGTTCGCTCTCATCTGGACTTTCTACTTCACCTACACTTCAGGTCTTGAAGAGGATGAGGAGTCAGGTCTATCCCTCTAG

Protein Analysis

137

Amino Acids

14.07

Weight (kDa)

5.24

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbW PF07123 1 - 136 2.7e-59 Photosystem II reaction centre W protein (PsbW)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015130)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30570
fragaria_vesca FvH4_2g22470
malus_domestica MD05G1205000.v1.1 MD10G1192100.v1.1
prunus_persica Prupe.8G231600_v2.0.a1
pyrus_communis pycom05g19120 pycom10g16600
rosa_chinensis RchiOBHm_Chr6g0289411
rosa_laevigata RLG00000012306
rosa_multiflora Rmu_sc0001527.1_g000032
rosa_rugosa Rorug06G0203300
rosa_samantha Rh6AG314300 Rh6BG321900 Rh6CG328100 Rh6DG313800
rosa_wichuraiana Rw6G027170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 71, 110
AclWI GGATC 2 cut(s) 313, 326
AcuI CTGAAG 1 cut(s) 354
AfiI CCNNNNNNNGG 3 cut(s) 71, 110, 160
AgsI TTSAA 2 cut(s) 138, 380
AjnI CCWGG 1 cut(s) 41
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
AluBI AGCT 5 cut(s) 17, 98, 127, 203, 209
AluI AGCT 5 cut(s) 17, 98, 127, 203, 209
Alw21I GWGCWC 2 cut(s) 60, 269
Alw44I GTGCAC 1 cut(s) 56
AlwI GGATC 2 cut(s) 313, 326
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 56
ApeKI GCWGC 5 cut(s) 47, 203, 206, 215, 236
AsuHPI GGTGA 2 cut(s) 31, 352
AsuNHI GCTAGC 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 60
BamHI GGATCC 1 cut(s) 318
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 2 cut(s) 60, 269
BbvI GCAGC 5 cut(s) 59, 190, 202, 218, 248
BccI CCATC 3 cut(s) 73, 88, 112
BciT130I CCWGG 1 cut(s) 43
BfaI CTAG 3 cut(s) 18, 95, 409
BfmI CTRYAG 1 cut(s) 204
BisI GCNGC 5 cut(s) 48, 204, 207, 216, 237
BlsI GCNGC 5 cut(s) 49, 205, 208, 217, 238
Bme1390I CCNGG 1 cut(s) 43
BmeT110I CYCGRG 1 cut(s) 311
BmiI GGNNCC 2 cut(s) 78, 320
BmrFI CCNGG 1 cut(s) 43
BmsI GCATC 1 cut(s) 200
BmtI GCTAGC 1 cut(s) 21
BplI GAGNNNNNCTC 4 cut(s) 155, 187, 178, 210
BsaJI CCNNGG 2 cut(s) 42, 240
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 110, 160
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 2 cut(s) 42, 240
BseGI GGATG 4 cut(s) 80, 189, 259, 391
BseLI CCNNNNNNNGG 3 cut(s) 71, 110, 160
BseMII CTCAG 1 cut(s) 119
BseRI GAGGAG 1 cut(s) 404
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 5 cut(s) 59, 190, 202, 218, 248
BseYI CCCAGC 1 cut(s) 232
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 60, 269
BsiHKCI CYCGRG 1 cut(s) 311
BslI CCNNNNNNNGG 3 cut(s) 71, 110, 160
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 3 cut(s) 60, 79, 269
Bsp143I GATC 1 cut(s) 318
Bsp19I CCATGG 1 cut(s) 240
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 78, 320
BspMAI CTGCAG 1 cut(s) 208
BspOI GCTAGC 1 cut(s) 21
BspPI GGATC 2 cut(s) 313, 326
BssECI CCNNGG 2 cut(s) 42, 240
BssMI GATC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 375
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 1 cut(s) 128
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 4 cut(s) 80, 189, 259, 391
BstKTI GATC 1 cut(s) 321
BstMBI GATC 1 cut(s) 318
BstMWI GCNNNNNNNGC 3 cut(s) 212, 215, 242
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 41
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 60
BstV1I GCAGC 5 cut(s) 59, 190, 202, 218, 248
BstX2I RGATCY 1 cut(s) 318
BstXI CCANNNNNNTGG 1 cut(s) 247
BstYI RGATCY 1 cut(s) 318
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 4 cut(s) 80, 189, 259, 391
Cac8I GCNNGC 1 cut(s) 19
CviAII CATG 2 cut(s) 223, 241
DdeI CTNAG 1 cut(s) 128
DpnI GATC 1 cut(s) 320
DpnII GATC 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 354
Eco88I CYCGRG 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 2 cut(s) 226, 244
FaiI YATR 2 cut(s) 224, 242
FatI CATG 2 cut(s) 222, 240
Fnu4HI GCNGC 5 cut(s) 48, 204, 207, 216, 237
FokI GGATG 4 cut(s) 67, 196, 266, 398
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 5 cut(s) 48, 204, 207, 216, 237
FspBI CTAG 3 cut(s) 18, 95, 409
GluI GCNGC 5 cut(s) 48, 204, 207, 216, 237
GsaI CCCAGC 1 cut(s) 236
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 2 cut(s) 226, 244
HinfI GANTC 1 cut(s) 392
HphI GGTGA 2 cut(s) 31, 352
Hpy166II GTNNAC 1 cut(s) 58
Hpy188III TCNNGA 2 cut(s) 346, 377
Hpy8I GTNNAC 1 cut(s) 58
HpyAV CCTTC 4 cut(s) 19, 154, 266, 332
HpyCH4V TGCA 4 cut(s) 58, 191, 206, 218
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 215, 242
HpyF3I CTNAG 1 cut(s) 128
Hsp92II CATG 2 cut(s) 226, 244
Kzo9I GATC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 8 cut(s) 28, 55, 182, 246, 264, 331, 357, 381
Lsp1109I GCAGC 5 cut(s) 59, 190, 202, 218, 248
LweI GCATC 1 cut(s) 200
MaeI CTAG 3 cut(s) 18, 95, 409
MaeIII GTNAC 1 cut(s) 37
MalI GATC 1 cut(s) 320
MboI GATC 1 cut(s) 318
MboII GAAGA 2 cut(s) 299, 392
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 3 cut(s) 60, 79, 269
MluCI AATT 1 cut(s) 108
MlyI GAGTC 1 cut(s) 401
MnlI CCTC 6 cut(s) 16, 40, 94, 136, 376, 382
MslI CAYNNNNRTG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 43
MunI CAATTG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 212, 215, 242
NcoI CCATGG 1 cut(s) 240
NdeII GATC 1 cut(s) 318
NheI GCTAGC 1 cut(s) 17
NlaIII CATG 2 cut(s) 226, 244
NlaIV GGNNCC 2 cut(s) 78, 320
NmuCI GTSAC 1 cut(s) 37
PflMI CCANNNNNTGG 2 cut(s) 71, 110
PkrI GCNGC 5 cut(s) 49, 205, 208, 217, 238
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
Psp6I CCWGG 1 cut(s) 41
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 2 cut(s) 78, 320
PstI CTGCAG 1 cut(s) 208
PsuI RGATCY 1 cut(s) 318
RseI CAYNNNNRTG 1 cut(s) 182
SatI GCNGC 5 cut(s) 48, 204, 207, 216, 237
Sau3AI GATC 1 cut(s) 318
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 3 cut(s) 60, 79, 269
SetI ASST 9 cut(s) 19, 32, 100, 129, 205, 211, 365, 376, 400
SfaNI GCATC 1 cut(s) 200
SfcI CTRYAG 1 cut(s) 204
SmiMI CAYNNNNRTG 1 cut(s) 182
Sse9I AATT 1 cut(s) 108
SspMI CTAG 3 cut(s) 18, 95, 409
StyD4I CCNGG 1 cut(s) 41
StyI CCWWGG 1 cut(s) 240
TasI AATT 1 cut(s) 108
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 5 cut(s) 47, 203, 206, 215, 236
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 173
Van91I CCANNNNNTGG 2 cut(s) 71, 110
VneI GTGCAC 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XspI CTAG 3 cut(s) 18, 95, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.