pycom10g16600

Photosystem II reaction centre W protein (PsbW)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015130
Physical Location & Seq
Forward (+)
19768771 .. 19769646
876 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g16600.1

Sequence Viewer

Length: 399 bp
ATGGCAACCATTACTGCTAGCACTGCAGCCTCGTCTGTCGTTAGGGCAAGCCTTGTGCACAAGCCATCTGCTGGGGCTCCATCCTCAACTGTTTTGGCTTTGCCGTCACTTGCGAGAAAGGGAAGAGTGAGCTGCTCTATGGAAGGAAAGAAGGAGAGCAACTCAAACATGGCCAAGGGTGGATCTTTGGCGGCAGCAGCTATGGCGGCGACCATGTCAAGCCCCGCTATGGCTTTGGTGGACGACAGATTGAGCACCGAGGGAACAGGGCTTCCGTTCGGATTGAGCAACAACCTTCTTGGTTGGATCCTCTTTGGGGTGTTTGGTCTCATCTGGGCACTCTACTTTACCTATACTTCCTCCCTTGAAGAGGACGAAGAGTCGGGACTGTCCCTCTGA

Protein Analysis

133

Amino Acids

13.52

Weight (kDa)

5.73

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbW PF07123 1 - 132 1e-61 Photosystem II reaction centre W protein (PsbW)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015130)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30570
fragaria_vesca FvH4_2g22470
malus_domestica MD05G1205000.v1.1 MD10G1192100.v1.1
prunus_persica Prupe.8G231600_v2.0.a1
pyrus_communis pycom05g19120 pycom10g16600
rosa_chinensis RchiOBHm_Chr6g0289411
rosa_laevigata RLG00000012306
rosa_multiflora Rmu_sc0001527.1_g000032
rosa_rugosa Rorug06G0203300
rosa_samantha Rh6AG314300 Rh6BG321900 Rh6CG328100 Rh6DG313800
rosa_wichuraiana Rw6G027170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 71
AciI CCGC 3 cut(s) 191, 206, 225
AclWI GGATC 3 cut(s) 190, 301, 314
AcoI YGGCCR 1 cut(s) 171
AfiI CCNNNNNNNGG 4 cut(s) 71, 229, 316, 370
AgsI TTSAA 1 cut(s) 368
AhdI GACNNNNNGTC 1 cut(s) 379
AloI GAACNNNNNNTCC 2 cut(s) 256, 288
AluBI AGCT 2 cut(s) 132, 200
AluI AGCT 2 cut(s) 132, 200
Alw21I GWGCWC 2 cut(s) 60, 257
Alw26I GTCTC 1 cut(s) 332
Alw44I GTGCAC 1 cut(s) 56
AlwI GGATC 3 cut(s) 190, 301, 314
AoxI GGCC 1 cut(s) 171
ApaLI GTGCAC 1 cut(s) 56
ApeKI GCWGC 4 cut(s) 26, 132, 194, 197
AsuNHI GCTAGC 1 cut(s) 17
BaeGI GKGCMC 2 cut(s) 60, 340
BalI TGGCCA 1 cut(s) 173
BamHI GGATCC 1 cut(s) 306
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 2 cut(s) 60, 257
BbvI GCAGC 4 cut(s) 38, 119, 206, 209
BccI CCATC 2 cut(s) 73, 88
BceAI ACGGC 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 6 cut(s) 27, 133, 192, 195, 198, 207
BlsI GCNGC 6 cut(s) 28, 134, 193, 196, 199, 208
BmeRI GACNNNNNGTC 1 cut(s) 379
BmiI GGNNCC 2 cut(s) 78, 308
BmtI GCTAGC 1 cut(s) 21
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 174, 258
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 229, 316, 370
BseDI CCNNGG 2 cut(s) 174, 258
BseGI GGATG 1 cut(s) 80
BseLI CCNNNNNNNGG 4 cut(s) 71, 229, 316, 370
BseSI GKGCMC 2 cut(s) 60, 340
BseXI GCAGC 4 cut(s) 38, 119, 206, 209
BseYI CCCAGC 1 cut(s) 71
BshFI GGCC 1 cut(s) 173
BsiHKAI GWGCWC 2 cut(s) 60, 257
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 4 cut(s) 71, 229, 316, 370
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 376
BsnI GGCC 1 cut(s) 173
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 4 cut(s) 60, 79, 257, 340
Bsp143I GATC 2 cut(s) 182, 306
BspACI CCGC 3 cut(s) 191, 206, 225
BspANI GGCC 1 cut(s) 173
BspLI GGNNCC 2 cut(s) 78, 308
BspMAI CTGCAG 1 cut(s) 28
BspOI GCTAGC 1 cut(s) 21
BspPI GGATC 3 cut(s) 190, 301, 314
BspTNI GGTCTC 1 cut(s) 332
BssECI CCNNGG 2 cut(s) 174, 258
BssMI GATC 2 cut(s) 182, 306
BssT1I CCWWGG 1 cut(s) 174
Bst4CI ACNGT 2 cut(s) 91, 390
Bst6I CTCTTC 3 cut(s) 118, 363, 372
BstC8I GCNNGC 2 cut(s) 19, 49
BstENI CCTNNNNNAGG 1 cut(s) 368
BstF5I GGATG 1 cut(s) 80
BstKTI GATC 2 cut(s) 185, 309
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 2 cut(s) 182, 306
BstMWI GCNNNNNNNGC 4 cut(s) 23, 197, 203, 206
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 2 cut(s) 60, 340
BstV1I GCAGC 4 cut(s) 38, 119, 206, 209
BstX2I RGATCY 2 cut(s) 182, 306
BstYI RGATCY 2 cut(s) 182, 306
BsuRI GGCC 1 cut(s) 173
BtsCI GGATG 1 cut(s) 80
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 2 cut(s) 19, 49
CviAII CATG 2 cut(s) 169, 214
DpnI GATC 2 cut(s) 184, 308
DpnII GATC 2 cut(s) 182, 306
DriI GACNNNNNGTC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 3 cut(s) 118, 363, 372
Eam1105I GACNNNNNGTC 1 cut(s) 379
EarI CTCTTC 3 cut(s) 118, 363, 372
Eco130I CCWWGG 1 cut(s) 174
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 2 cut(s) 172, 217
FaiI YATR 6 cut(s) 140, 170, 203, 215, 230, 354
FaqI GGGAC 1 cut(s) 376
FatI CATG 2 cut(s) 168, 213
FauI CCCGC 1 cut(s) 232
Fnu4HI GCNGC 6 cut(s) 27, 133, 192, 195, 198, 207
FokI GGATG 1 cut(s) 67
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 6 cut(s) 27, 133, 192, 195, 198, 207
FspBI CTAG 1 cut(s) 18
GluI GCNGC 6 cut(s) 27, 133, 192, 195, 198, 207
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 1 cut(s) 173
Hin1II CATG 2 cut(s) 172, 217
HinfI GANTC 1 cut(s) 380
Hpy166II GTNNAC 2 cut(s) 58, 241
Hpy188I TCNGA 2 cut(s) 281, 398
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 2 cut(s) 58, 241
HpyAV CCTTC 3 cut(s) 137, 145, 305
HpyCH4III ACNGT 2 cut(s) 91, 390
HpyCH4V TGCA 2 cut(s) 26, 58
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 197, 203, 206
Hsp92II CATG 2 cut(s) 172, 217
Kzo9I GATC 2 cut(s) 182, 306
LmnI GCTCC 1 cut(s) 82
LpnPI CCDG 3 cut(s) 57, 252, 319
Lsp1109I GCAGC 4 cut(s) 38, 119, 206, 209
MaeI CTAG 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 105
MalI GATC 2 cut(s) 184, 308
MboI GATC 2 cut(s) 182, 306
MboII GAAGA 3 cut(s) 135, 380, 389
MflI RGATCY 2 cut(s) 182, 306
MhlI GDGCHC 4 cut(s) 60, 79, 257, 340
MlsI TGGCCA 1 cut(s) 173
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 6 cut(s) 40, 94, 253, 320, 364, 370
Mox20I TGGCCA 1 cut(s) 173
MscI TGGCCA 1 cut(s) 173
Msp20I TGGCCA 1 cut(s) 173
MwoI GCNNNNNNNGC 4 cut(s) 23, 197, 203, 206
NdeII GATC 2 cut(s) 182, 306
NheI GCTAGC 1 cut(s) 17
NlaIII CATG 2 cut(s) 172, 217
NlaIV GGNNCC 2 cut(s) 78, 308
NmuCI GTSAC 1 cut(s) 105
PflFI GACNNNGTC 1 cut(s) 214
PflMI CCANNNNNTGG 1 cut(s) 71
PkrI GCNGC 6 cut(s) 28, 134, 193, 196, 199, 208
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PspFI CCCAGC 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 78, 308
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 2 cut(s) 182, 306
PsyI GACNNNGTC 1 cut(s) 214
SatI GCNGC 6 cut(s) 27, 133, 192, 195, 198, 207
Sau3AI GATC 2 cut(s) 182, 306
SchI GAGTC 1 cut(s) 389
SduI GDGCHC 4 cut(s) 60, 79, 257, 340
SetI ASST 4 cut(s) 134, 202, 297, 353
SfcI CTRYAG 1 cut(s) 24
SsiI CCGC 3 cut(s) 191, 206, 225
SspMI CTAG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 91, 390
TauI GCSGC 2 cut(s) 194, 209
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 1 cut(s) 105
TseI GCWGC 4 cut(s) 26, 132, 194, 197
Tsp45I GTSAC 1 cut(s) 105
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 1 cut(s) 28
Tth111I GACNNNGTC 1 cut(s) 214
Van91I CCANNNNNTGG 1 cut(s) 71
VneI GTGCAC 1 cut(s) 56
XagI CCTNNNNNAGG 1 cut(s) 368
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.