FvH4_2g25230

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015846
Physical Location & Seq
Reverse (-)
20545071 .. 20546471
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25230.t1

Sequence Viewer

Length: 1401 bp
ATGTCGCAGTTCAACGTCGCAACCACCAGACTCCTCTCCCTCCTACCCAACTGCTCCTCCGCGGCTCACATCGCCCAAATCCAAGCCCACCTCATCACCCAAAACCTGCACTCCATCACCACCGTCGCCTCCGCCTTCATCTCCGCCTCTAAAACCCTTAACCTCCTCCACTTAGCCCACCTCCACATCCTCACCCAGCTCCCAACCCCCCATGTCTTCACCTGCAACTCCCTCATCAGAGCCTTCTCTCACTCCCACATCCCCCACATCCCTCTCTCCATCTACACCCACATGACCAGAACCTCCATTGCCCCCAACAACTACACCTTCCCTATTCTCTTCAAGTCCTTGGCCGACTTCCGGGACCTCAGACAAGGCCAATGCCTGCATACCCATGTCGTCAAATTGGGGCAGTGGAGTGATATATATGTCTTAAACTCTGCTTTGGATTTGTATGCTTCGTGTGGCAGGATGGACCTGTGTCGAAAGGTGTTTGATGAAATGCCTGTGAGAGATGTTGTGTCATGGACCGGGTTGATTAAGGGGTATAGGAGATTGGGGAAGTATGACGATGCGTTGATTGCTTTTGAGCAGATGCAGTATGCTGGTGTGGCGCCGAATGCTGTGACGATGGTGAATGCTTTGGCTGCGTGTGCGAGCTTTGGGGCGCTGGAGATGGGGGTGTGGATTCATGAGTGTATAAGGAGGGAGGGGTGGGAGTTGGATGTGAAATTGGGGACTGCGTTGATTGATATGTACGGAACATGTGGGAGAATTGAGGAGGCTTTGGTTGTTTTCGATGGGATGGAAGAGAAGAACACATTTACTTGGAATGCACTTATTAAGGGGTTTGCTCTTGCAAAGAGCGGTGAGGAGGCGGTTTGGTGGTTCGGGAGGATGGAGCAAGAAAGGTTTGGTGCAGACGAGGTGACTTTGGTGCAAGTGCTTTGTGCTTGTAGTCACTCGGGCTTGGTAGATGTTGGTAGACAGATTTTTTGTTCCTTGATCAATGGGAAGTATGGATTTTTGCCTAGCGTTAAGCATTATGCTTGTATGATCGACCTCTTGGCTCGTGCCGGTTGTCTTGAAGAGGCTGTCAAATGCTTGAGAGAAATGTCTTATCAGCCTACTAAAGCTATTTTGGGGTCATTGCTGGCTGCTGGTCAAACTCATGGGGACTTGCCATTGAGCGAGTTTGCAGCTGCAAAGCTTGTTGAACTGGAGCCGGGGAATAGTGCTTACTATGTCTTGTTGTCGAATTTATATGCCGAGATGGGAAGATGGAATGATGTTGAGAAAGTGAGGGCGATGATGAAAGAGAGAGACCTTAAGAAAGAATTGGGTTGTAGCTCGACGGAGCGTGAATTCTGTAACCAAGTTGATCAACTAGTAGCGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00630 ko00910 ko01200 map00460 map00630 map00910 map01200
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

467

Amino Acids

51.88

Weight (kDa)

6.38

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 70 - 118 1.3e-06 PPR repeat family
PPR_2 PF13041 171 - 217 3.9e-08 PPR repeat family
E_motif PF20431 391 - 451 2.7e-21 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015846)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43790
fragaria_vesca FvH4_2g25230
malus_domestica MD03G1282600.v1.1 MD11G1303700.v1.1
prunus_persica Prupe.8G257000_v2.0.a1
pyrus_communis pycom11g26530
rosa_chinensis RchiOBHm_Chr6g0293351
rosa_laevigata RLG00000011953
rosa_multiflora Rmu_sc0006269.1_g000030
rosa_roxburghii Rroxscaffold_7G00174230
rosa_rugosa Rorug06G0234800
rosa_samantha Rh6AG347700 Rh6BG355300 Rh6DG348500
rosa_wichuraiana Rw6G030250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 230
Acc36I ACCTGC 2 cut(s) 114, 230
AccB1I GGYRCC 1 cut(s) 613
AccBSI CCGCTC 1 cut(s) 867
AccI GTMKAC 1 cut(s) 985
AccII CGCG 1 cut(s) 62
AciI CCGC 6 cut(s) 60, 62, 132, 144, 867, 878
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 2 cut(s) 1258, 1364
AcyI GRCGYC 1 cut(s) 614
AfaI GTAC 1 cut(s) 758
AfiI CCNNNNNNNGG 1 cut(s) 360
AflII CTTAAG 1 cut(s) 1328
AflIII ACRYGT 1 cut(s) 764
AgsI TTSAA 4 cut(s) 13, 343, 1088, 1217
AhlI ACTAGT 1 cut(s) 1387
AluBI AGCT 6 cut(s) 199, 660, 1136, 1202, 1210, 1350
AluI AGCT 6 cut(s) 199, 660, 1136, 1202, 1210, 1350
Alw26I GTCTC 1 cut(s) 1317
Ama87I CYCGRG 1 cut(s) 964
AoxI GGCC 2 cut(s) 351, 376
ApeKI GCWGC 4 cut(s) 647, 1157, 1199, 1202
ApoI RAATTY 2 cut(s) 1258, 1364
ArsI GACNNNNNNTTYG 2 cut(s) 978, 1010
AspLEI GCGC 3 cut(s) 616, 670, 1396
AspS9I GGNCC 3 cut(s) 364, 475, 528
AsuC2I CCSGG 3 cut(s) 362, 532, 1227
AsuHPI GGTGA 7 cut(s) 88, 109, 184, 211, 646, 881, 940
AvaI CYCGRG 1 cut(s) 964
AvaII GGWCC 3 cut(s) 364, 475, 528
BanI GGYRCC 1 cut(s) 613
BauI CACGAG 1 cut(s) 1071
BbsI GAAGAC 1 cut(s) 208
BbvI GCAGC 4 cut(s) 634, 1144, 1189, 1211
BclI TGATCA 2 cut(s) 1005, 1381
BcnI CCSGG 3 cut(s) 362, 532, 1227
BcoDI GTCTC 1 cut(s) 1317
BcuI ACTAGT 1 cut(s) 1387
BfaI CTAG 2 cut(s) 1032, 1388
BfoI RGCGCY 2 cut(s) 617, 671
BfrI CTTAAG 1 cut(s) 1328
BfuAI ACCTGC 2 cut(s) 114, 230
BisI GCNGC 5 cut(s) 63, 648, 1158, 1200, 1203
BlsI GCNGC 5 cut(s) 64, 649, 1159, 1201, 1204
Bme1390I CCNGG 3 cut(s) 362, 532, 1227
Bme18I GGWCC 3 cut(s) 364, 475, 528
BmeT110I CYCGRG 1 cut(s) 964
BmgT120I GGNCC 3 cut(s) 364, 475, 528
BmiI GGNNCC 3 cut(s) 365, 615, 1224
BmrFI CCNGG 3 cut(s) 362, 532, 1227
BmsI GCATC 2 cut(s) 562, 585
BoxI GACNNNNGTC 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 208
BpmI CTGGAG 2 cut(s) 692, 1241
BpuEI CTTGAG 1 cut(s) 1126
BpuMI CCSGG 3 cut(s) 362, 532, 1227
BsaHI GRCGYC 1 cut(s) 614
BsaI GGTCTC 1 cut(s) 1317
BsaJI CCNNGG 3 cut(s) 60, 348, 1226
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 95, 125
Bsc4I CCNNNNNNNGG 1 cut(s) 360
Bse118I RCCGGY 1 cut(s) 1076
Bse1I ACTGG 1 cut(s) 1224
Bse3DI GCAATG 2 cut(s) 306, 1148
BseDI CCNNGG 3 cut(s) 60, 348, 1226
BseGI GGATG 7 cut(s) 186, 258, 267, 477, 730, 810, 903
BseLI CCNNNNNNNGG 1 cut(s) 360
BseMI GCAATG 2 cut(s) 306, 1148
BseMII CTCAG 1 cut(s) 382
BseNI ACTGG 1 cut(s) 1224
BseRI GAGGAG 5 cut(s) 23, 46, 155, 794, 887
BseXI GCAGC 4 cut(s) 634, 1144, 1189, 1211
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 2 cut(s) 92, 939
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 2 cut(s) 353, 378
BshNI GGYRCC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 964
BsiSI CCGG 4 cut(s) 361, 531, 1077, 1226
BslFI GGGAC 3 cut(s) 377, 751, 1190
BslI CCNNNNNNNGG 1 cut(s) 360
BsmAI GTCTC 1 cut(s) 1317
BsmFI GGGAC 3 cut(s) 377, 751, 1190
BsmI GAATGC 3 cut(s) 625, 643, 838
BsnI GGCC 2 cut(s) 353, 378
Bso31I GGTCTC 1 cut(s) 1317
BsoBI CYCGRG 1 cut(s) 964
Bsp143I GATC 3 cut(s) 1005, 1056, 1381
BspACI CCGC 6 cut(s) 60, 62, 132, 144, 867, 878
BspANI GGCC 2 cut(s) 353, 378
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 691
BspLI GGNNCC 3 cut(s) 365, 615, 1224
BspMI ACCTGC 2 cut(s) 114, 230
BspT107I GGYRCC 1 cut(s) 613
BspTI CTTAAG 1 cut(s) 1328
BspTNI GGTCTC 1 cut(s) 1317
BsrBI CCGCTC 1 cut(s) 867
BsrDI GCAATG 2 cut(s) 306, 1148
BsrFI RCCGGY 1 cut(s) 1076
BsrI ACTGG 1 cut(s) 1224
BssAI RCCGGY 1 cut(s) 1076
BssECI CCNNGG 3 cut(s) 60, 348, 1226
BssMI GATC 3 cut(s) 1005, 1056, 1381
BssNI GRCGYC 1 cut(s) 614
BssSI CACGAG 1 cut(s) 1071
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 1071
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 3 cut(s) 344, 804, 1083
BstACI GRCGYC 1 cut(s) 614
BstAFI CTTAAG 1 cut(s) 1328
BstC8I GCNNGC 3 cut(s) 386, 658, 1155
BstDEI CTNAG 2 cut(s) 172, 368
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 7 cut(s) 186, 258, 267, 477, 730, 810, 903
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 2 cut(s) 617, 671
BstHHI GCGC 3 cut(s) 616, 670, 1396
BstKTI GATC 3 cut(s) 1008, 1059, 1384
BstMAI GTCTC 1 cut(s) 1317
BstMBI GATC 3 cut(s) 1005, 1056, 1381
BstMWI GCNNNNNNNGC 6 cut(s) 71, 581, 611, 620, 647, 653
BstNSI RCATGY 1 cut(s) 768
BstPAI GACNNNNGTC 1 cut(s) 480
BstSCI CCNGG 3 cut(s) 360, 530, 1225
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 4 cut(s) 634, 1144, 1189, 1211
BstV2I GAAGAC 1 cut(s) 208
BsuRI GGCC 2 cut(s) 353, 378
BtgI CCRYGG 1 cut(s) 60
BtgZI GCGATG 2 cut(s) 55, 1322
BtsCI GGATG 7 cut(s) 186, 258, 267, 477, 730, 810, 903
BtsI GCAGTG 1 cut(s) 419
BtsIMutI CAGTG 1 cut(s) 419
BveI ACCTGC 2 cut(s) 114, 230
Cac8I GCNNGC 3 cut(s) 386, 658, 1155
CciI TCATGA 1 cut(s) 691
CfoI GCGC 3 cut(s) 616, 670, 1396
Cfr10I RCCGGY 1 cut(s) 1076
Cfr13I GGNCC 3 cut(s) 364, 475, 528
Cfr42I CCGCGG 1 cut(s) 63
Csp6I GTAC 1 cut(s) 757
CviAII CATG 7 cut(s) 212, 292, 395, 525, 692, 765, 1172
CviQI GTAC 1 cut(s) 757
DdeI CTNAG 2 cut(s) 172, 368
DinI GGCGCC 1 cut(s) 615
DpnI GATC 3 cut(s) 1007, 1058, 1383
DpnII GATC 3 cut(s) 1005, 1056, 1381
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 3 cut(s) 344, 804, 1083
EarI CTCTTC 3 cut(s) 344, 804, 1083
EciI GGCGGA 2 cut(s) 121, 133
Eco130I CCWWGG 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 1317
Eco47I GGWCC 3 cut(s) 364, 475, 528
Eco88I CYCGRG 1 cut(s) 964
EcoO109I RGGNCCY 1 cut(s) 364
EcoRI GAATTC 1 cut(s) 1364
EcoT14I CCWWGG 1 cut(s) 348
EgeI GGCGCC 1 cut(s) 615
EheI GGCGCC 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 7 cut(s) 215, 295, 398, 528, 695, 768, 1175
FaqI GGGAC 3 cut(s) 377, 751, 1190
FatI CATG 7 cut(s) 211, 291, 394, 524, 691, 764, 1171
FbaI TGATCA 2 cut(s) 1005, 1381
FblI GTMKAC 1 cut(s) 985
Fnu4HI GCNGC 5 cut(s) 63, 648, 1158, 1200, 1203
FokI GGATG 7 cut(s) 173, 245, 254, 484, 737, 817, 910
Fsp4HI GCNGC 5 cut(s) 63, 648, 1158, 1200, 1203
FspBI CTAG 2 cut(s) 1032, 1388
GlaI GCGC 3 cut(s) 615, 669, 1395
GluI GCNGC 5 cut(s) 63, 648, 1158, 1200, 1203
GsaI CCCAGC 1 cut(s) 199
GsuI CTGGAG 2 cut(s) 692, 1241
HaeII RGCGCY 2 cut(s) 617, 671
HaeIII GGCC 2 cut(s) 353, 378
HapII CCGG 4 cut(s) 361, 531, 1077, 1226
HhaI GCGC 3 cut(s) 616, 670, 1396
Hin1I GRCGYC 1 cut(s) 614
Hin1II CATG 7 cut(s) 215, 295, 398, 528, 695, 768, 1175
Hin6I GCGC 3 cut(s) 614, 668, 1394
HinP1I GCGC 3 cut(s) 614, 668, 1394
HindIII AAGCTT 1 cut(s) 1208
HinfI GANTC 2 cut(s) 30, 688
HpaII CCGG 4 cut(s) 361, 531, 1077, 1226
HphI GGTGA 7 cut(s) 88, 109, 184, 211, 646, 881, 940
Hpy166II GTNNAC 1 cut(s) 986
Hpy188I TCNGA 2 cut(s) 239, 371
Hpy188III TCNNGA 3 cut(s) 692, 892, 1085
Hpy8I GTNNAC 1 cut(s) 986
Hpy99I CGWCG 3 cut(s) 20, 128, 1357
HpyAV CCTTC 3 cut(s) 145, 253, 337
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 1 cut(s) 15
HpyF10VI GCNNNNNNNGC 6 cut(s) 71, 581, 611, 620, 647, 653
HpyF3I CTNAG 2 cut(s) 172, 368
HpySE526I ACGT 1 cut(s) 15
Hsp92I GRCGYC 1 cut(s) 614
Hsp92II CATG 7 cut(s) 215, 295, 398, 528, 695, 768, 1175
HspAI GCGC 3 cut(s) 614, 668, 1394
KasI GGCGCC 1 cut(s) 613
Ksp22I TGATCA 2 cut(s) 1005, 1381
KspI CCGCGG 1 cut(s) 63
Kzo9I GATC 3 cut(s) 1005, 1056, 1381
LmnI GCTCC 5 cut(s) 59, 204, 901, 1222, 1357
Lsp1109I GCAGC 4 cut(s) 634, 1144, 1189, 1211
LweI GCATC 2 cut(s) 562, 585
MaeI CTAG 2 cut(s) 1032, 1388
MaeII ACGT 1 cut(s) 15
MaeIII GTNAC 4 cut(s) 625, 928, 959, 1370
MalI GATC 3 cut(s) 1007, 1058, 1383
MbiI CCGCTC 1 cut(s) 867
MboI GATC 3 cut(s) 1005, 1056, 1381
MboII GAAGA 6 cut(s) 208, 331, 821, 826, 1100, 1290
MluCI AATT 6 cut(s) 404, 731, 774, 1258, 1337, 1364
Mly113I GGCGCC 1 cut(s) 614
MlyI GAGTC 1 cut(s) 24
MmeI TCCRAC 1 cut(s) 702
MseI TTAA 6 cut(s) 159, 434, 540, 843, 1038, 1329
MslI CAYNNNNRTG 2 cut(s) 290, 393
MspA1I CMGCKG 2 cut(s) 62, 1202
MspCI CTTAAG 1 cut(s) 1328
MspI CCGG 4 cut(s) 361, 531, 1077, 1226
MspR9I CCNGG 3 cut(s) 362, 532, 1227
Mva1269I GAATGC 3 cut(s) 625, 643, 838
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 6 cut(s) 71, 581, 611, 620, 647, 653
NarI GGCGCC 1 cut(s) 614
NciI CCSGG 3 cut(s) 362, 532, 1227
NdeII GATC 3 cut(s) 1005, 1056, 1381
NlaIII CATG 7 cut(s) 215, 295, 398, 528, 695, 768, 1175
NlaIV GGNNCC 3 cut(s) 365, 615, 1224
NmeAIII GCCGAG 1 cut(s) 1294
NmuCI GTSAC 3 cut(s) 625, 928, 959
NspI RCATGY 1 cut(s) 768
PagI TCATGA 1 cut(s) 691
PaqCI CACCTGC 1 cut(s) 230
PciI ACATGT 1 cut(s) 764
PctI GAATGC 3 cut(s) 625, 643, 838
PfeI GAWTC 1 cut(s) 688
PfoI TCCNGGA 1 cut(s) 360
PkrI GCNGC 5 cut(s) 64, 649, 1159, 1201, 1204
PleI GAGTC 1 cut(s) 24
PluTI GGCGCC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 24
PpuMI RGGWCCY 1 cut(s) 364
PscI ACATGT 1 cut(s) 764
PshAI GACNNNNGTC 1 cut(s) 480
Psp5II RGGWCCY 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 195
PspN4I GGNNCC 3 cut(s) 365, 615, 1224
PspPI GGNCC 3 cut(s) 364, 475, 528
PspPPI RGGWCCY 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 1202
RsaI GTAC 1 cut(s) 758
RsaNI GTAC 1 cut(s) 757
RseI CAYNNNNRTG 2 cut(s) 290, 393
SacII CCGCGG 1 cut(s) 63
SaqAI TTAA 6 cut(s) 159, 434, 540, 843, 1038, 1329
SatI GCNGC 5 cut(s) 63, 648, 1158, 1200, 1203
Sau3AI GATC 3 cut(s) 1005, 1056, 1381
Sau96I GGNCC 3 cut(s) 364, 475, 528
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 3 cut(s) 362, 532, 1227
SfaNI GCATC 2 cut(s) 562, 585
SfoI GGCGCC 1 cut(s) 615
Sfr303I CCGCGG 1 cut(s) 63
SgrBI CCGCGG 1 cut(s) 63
SinI GGWCC 3 cut(s) 364, 475, 528
SmiMI CAYNNNNRTG 2 cut(s) 290, 393
SmlI CTYRAG 2 cut(s) 1105, 1328
SmoI CTYRAG 2 cut(s) 1105, 1328
SpeI ACTAGT 1 cut(s) 1387
Sse9I AATT 6 cut(s) 404, 731, 774, 1258, 1337, 1364
SsiI CCGC 6 cut(s) 60, 62, 132, 144, 867, 878
SspDI GGCGCC 1 cut(s) 613
SspMI CTAG 2 cut(s) 1032, 1388
StyD4I CCNGG 3 cut(s) 360, 530, 1225
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 1 cut(s) 18
TaqI TCGA 5 cut(s) 484, 798, 1059, 1256, 1352
TasI AATT 6 cut(s) 404, 731, 774, 1258, 1337, 1364
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 1 cut(s) 688
Tru1I TTAA 6 cut(s) 159, 434, 540, 843, 1038, 1329
Tru9I TTAA 6 cut(s) 159, 434, 540, 843, 1038, 1329
TscAI CASTG 1 cut(s) 419
TseFI GTSAC 3 cut(s) 625, 928, 959
TseI GCWGC 4 cut(s) 647, 1157, 1199, 1202
Tsp45I GTSAC 3 cut(s) 625, 928, 959
TspDTI ATGAA 4 cut(s) 127, 513, 680, 1328
TspGWI ACGGA 2 cut(s) 774, 1370
TspRI CASTG 1 cut(s) 419
Vha464I CTTAAG 1 cut(s) 1328
VpaK11BI GGWCC 3 cut(s) 364, 475, 528
XapI RAATTY 2 cut(s) 1258, 1364
XceI RCATGY 1 cut(s) 768
XmiI GTMKAC 1 cut(s) 985
XspI CTAG 2 cut(s) 1032, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.