FvH4_2g25710

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014556
Physical Location & Seq
Forward (+)
20772372 .. 20773854
1483 bp
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UTR
Exon/CDS
Intron
FvH4_2g25710.t1

Sequence Viewer

Length: 594 bp
ATGGCGTACGCCAACATTTTTGTGACATTCATTTTTTTTGCATTTCTGACCATTCCAACTCCTTTGACAGCAGGAAATGGGGATCATAGCTATGTGGAACACGCCTGCAGCGTGACGAGGTACCGTGACCTCTGCATCCGCTCACTAGCATCATTTTCAAGCACAGCAAAGAATAGCCCCAGCAGGTGGGCTAGAGCCGGGGTTTCGGTGACATTGGGGGAGACCAAAATCGTTACTCGGTACTTGATCACTCTCAAGAAGCACAACGGAATGAAGGGAAAATTTGGAGTTGCTCTATCTGATTGCATGGAGTGTTTCGACAATGCCATTGATGAGCTTCACAAATCGCTTGGTGTACTAAGAAAGCTGAGCAGAACCACATTCGTCACGCAAATGGGGGACCTTAACACATGGCTGAGTGCCGCGCTTACTGACGGAAACACTTGCTTGGACGGTTTTGAGGAGGACCGAAAAGGAAACCAACTCAAATCGCTTAAAAATCGGGTTTTGAGAGCAACTCATTTCACCAGTAACGCACTGGCTCTTGCTAACAAACTTGCTGCCACTGGCTTGGAAAGCTTCCCTGATCCATAG

Protein Analysis

198

Amino Acids

21.64

Weight (kDa)

9.16

Isoelectric Point (pI)

28.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 31 - 182 2.9e-35 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014556)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47670 AT3G62820
fragaria_vesca FvH4_2g25710
malus_domestica MD03G1288100.v1.1 MD11G1310200.v1.1
prunus_persica Prupe.8G261300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0293991
rosa_laevigata RLG00000011909
rosa_multiflora Rmu_sc0005126.1_g000006 Rmu_sc0005647.1_g000002
rosa_roxburghii Rroxscaffold_7G00173760
rosa_rugosa Rorug06G0239900
rosa_samantha Rh6AG352900 Rh6BG359900 Rh6CG367200 Rh6DG353600
rosa_wichuraiana Rw6G030750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 174
Acc65I GGTACC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 120
AccB7I CCANNNNNTGG 1 cut(s) 186
AccBSI CCGCTC 1 cut(s) 141
AccII CGCG 1 cut(s) 425
AciI CCGC 2 cut(s) 139, 423
AclWI GGATC 2 cut(s) 90, 581
AcsI RAATTY 1 cut(s) 281
AfaI GTAC 4 cut(s) 8, 122, 242, 357
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 1 cut(s) 159
AluBI AGCT 4 cut(s) 90, 337, 367, 579
AluI AGCT 4 cut(s) 90, 337, 367, 579
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 2 cut(s) 90, 581
ApeKI GCWGC 2 cut(s) 108, 560
ApoI RAATTY 1 cut(s) 281
Asp718I GGTACC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 400, 466
AsuC2I CCSGG 1 cut(s) 199
AsuHPI GGTGA 2 cut(s) 220, 517
AvaII GGWCC 2 cut(s) 400, 466
BanI GGYRCC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 120, 547
BclI TGATCA 1 cut(s) 246
BcnI CCSGG 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 2 cut(s) 146, 192
BfmI CTRYAG 1 cut(s) 106
BfuAI ACCTGC 1 cut(s) 174
BisI GCNGC 3 cut(s) 109, 423, 561
BlpI GCTNAGC 1 cut(s) 368
BlsI GCNGC 3 cut(s) 110, 424, 562
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 2 cut(s) 400, 466
BmgT120I GGNCC 2 cut(s) 400, 466
BmiI GGNNCC 2 cut(s) 122, 401
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 2 cut(s) 144, 158
BplI GAGNNNNNCTC 2 cut(s) 502, 534
Bpu1102I GCTNAGC 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 199
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 198
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 3 cut(s) 528, 543, 571
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 359, 407
BseNI ACTGG 3 cut(s) 528, 543, 571
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 2 cut(s) 120, 547
BseYI CCCAGC 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 425
BshNI GGYRCC 1 cut(s) 120
BsiSI CCGG 1 cut(s) 198
BsiWI CGTACG 1 cut(s) 6
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 413
Bso31I GGTCTC 1 cut(s) 215
Bsp143I GATC 3 cut(s) 82, 246, 586
Bsp1720I GCTNAGC 1 cut(s) 368
BspACI CCGC 2 cut(s) 139, 423
BspCNI CTCAG 2 cut(s) 360, 408
BspFNI CGCG 1 cut(s) 425
BspLI GGNNCC 2 cut(s) 122, 401
BspMAI CTGCAG 1 cut(s) 110
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 2 cut(s) 90, 581
BspT107I GGYRCC 1 cut(s) 120
BspTNI GGTCTC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 141
BsrI ACTGG 3 cut(s) 528, 543, 571
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 3 cut(s) 82, 246, 586
Bst4CI ACNGT 2 cut(s) 125, 455
BstC8I GCNNGC 1 cut(s) 106
BstDEI CTNAG 3 cut(s) 359, 368, 416
BstF5I GGATG 1 cut(s) 135
BstFNI CGCG 1 cut(s) 425
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 3 cut(s) 85, 249, 589
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 3 cut(s) 82, 246, 586
BstMWI GCNNNNNNNGC 1 cut(s) 576
BstSCI CCNGG 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 106
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 120, 547
BstXI CCANNNNNNTGG 1 cut(s) 571
BtsCI GGATG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 536, 564
BveI ACCTGC 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 106
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 2 cut(s) 400, 466
Csp6I GTAC 4 cut(s) 7, 121, 241, 356
CviAII CATG 2 cut(s) 307, 411
CviQI GTAC 4 cut(s) 7, 121, 241, 356
DdeI CTNAG 3 cut(s) 359, 368, 416
DpnI GATC 3 cut(s) 84, 248, 588
DpnII GATC 3 cut(s) 82, 246, 586
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 2 cut(s) 400, 466
EcoO109I RGGNCCY 1 cut(s) 400
FaeI CATG 2 cut(s) 310, 414
FaiI YATR 5 cut(s) 87, 93, 308, 412, 592
FaqI GGGAC 1 cut(s) 413
FatI CATG 2 cut(s) 306, 410
FbaI TGATCA 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 109, 423, 561
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 3 cut(s) 109, 423, 561
FspBI CTAG 2 cut(s) 146, 192
GlaI GCGC 1 cut(s) 426
GluI GCNGC 3 cut(s) 109, 423, 561
GsaI CCCAGC 1 cut(s) 183
HapII CCGG 1 cut(s) 198
HhaI GCGC 1 cut(s) 427
Hin1II CATG 2 cut(s) 310, 414
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HindIII AAGCTT 1 cut(s) 577
HpaII CCGG 1 cut(s) 198
HphI GGTGA 2 cut(s) 220, 517
Hpy166II GTNNAC 1 cut(s) 356
Hpy188I TCNGA 2 cut(s) 48, 301
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 1 cut(s) 356
HpyAV CCTTC 1 cut(s) 268
HpyCH4III ACNGT 2 cut(s) 125, 455
HpyCH4V TGCA 4 cut(s) 41, 108, 135, 306
HpyF10VI GCNNNNNNNGC 1 cut(s) 576
HpyF3I CTNAG 3 cut(s) 359, 368, 416
Hsp92II CATG 2 cut(s) 310, 414
HspAI GCGC 1 cut(s) 425
KpnI GGTACC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 3 cut(s) 82, 246, 586
LpnPI CCDG 8 cut(s) 57, 118, 169, 193, 211, 524, 541, 552
Lsp1109I GCAGC 2 cut(s) 120, 547
LweI GCATC 2 cut(s) 144, 158
MaeI CTAG 2 cut(s) 146, 192
MaeIII GTNAC 7 cut(s) 22, 112, 125, 208, 232, 385, 530
MalI GATC 3 cut(s) 84, 248, 588
MbiI CCGCTC 1 cut(s) 141
MboI GATC 3 cut(s) 82, 246, 586
MluCI AATT 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 4 cut(s) 111, 140, 454, 457
MseI TTAA 2 cut(s) 405, 495
MslI CAYNNNNRTG 3 cut(s) 20, 90, 392
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 1 cut(s) 199
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 1 cut(s) 576
NciI CCSGG 1 cut(s) 199
NdeII GATC 3 cut(s) 82, 246, 586
NlaIII CATG 2 cut(s) 310, 414
NlaIV GGNNCC 2 cut(s) 122, 401
NmuCI GTSAC 5 cut(s) 22, 112, 125, 208, 385
PaqCI CACCTGC 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 108
Pfl23II CGTACG 1 cut(s) 6
PflMI CCANNNNNTGG 1 cut(s) 186
PkrI GCNGC 3 cut(s) 110, 424, 562
PpuMI RGGWCCY 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 179
PspLI CGTACG 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 122, 401
PspPI GGNCC 2 cut(s) 400, 466
PspPPI RGGWCCY 1 cut(s) 400
PstI CTGCAG 1 cut(s) 110
RsaI GTAC 4 cut(s) 8, 122, 242, 357
RsaNI GTAC 4 cut(s) 7, 121, 241, 356
RseI CAYNNNNRTG 3 cut(s) 20, 90, 392
SaqAI TTAA 2 cut(s) 405, 495
SatI GCNGC 3 cut(s) 109, 423, 561
Sau3AI GATC 3 cut(s) 82, 246, 586
Sau96I GGNCC 2 cut(s) 400, 466
ScrFI CCNGG 1 cut(s) 199
SetI ASST 8 cut(s) 92, 122, 132, 188, 339, 369, 405, 581
SfaNI GCATC 2 cut(s) 144, 158
SfcI CTRYAG 1 cut(s) 106
SinI GGWCC 2 cut(s) 400, 466
SmiMI CAYNNNNRTG 3 cut(s) 20, 90, 392
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 1 cut(s) 281
SsiI CCGC 2 cut(s) 139, 423
SspMI CTAG 2 cut(s) 146, 192
StyD4I CCNGG 1 cut(s) 197
TaaI ACNGT 2 cut(s) 125, 455
TaqI TCGA 1 cut(s) 318
TaqII GACCGA 1 cut(s) 483
TasI AATT 1 cut(s) 281
TatI WGTACW 1 cut(s) 355
TauI GCSGC 1 cut(s) 425
Tru1I TTAA 2 cut(s) 405, 495
Tru9I TTAA 2 cut(s) 405, 495
TscAI CASTG 2 cut(s) 543, 571
TseFI GTSAC 5 cut(s) 22, 112, 125, 208, 385
TseI GCWGC 2 cut(s) 108, 560
Tsp45I GTSAC 5 cut(s) 22, 112, 125, 208, 385
TspDTI ATGAA 2 cut(s) 19, 287
TspGWI ACGGA 2 cut(s) 282, 450
TspRI CASTG 2 cut(s) 543, 571
Van91I CCANNNNNTGG 1 cut(s) 186
VpaK11BI GGWCC 2 cut(s) 400, 466
XapI RAATTY 1 cut(s) 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XspI CTAG 2 cut(s) 146, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.