MD11G1310200.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014556
Physical Location & Seq
Reverse (-)
42338076 .. 42339158
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1310200.v1.1.491

Sequence Viewer

Length: 636 bp
ATGAAAATGGCATCTTGTAGCAGTTATATTTTTGTGTCACTCATTTTTCATGCAATATGGCTGATTTGTTCAACCCAATTATTGGTATCTGGAAAGGGGGACAAAACTTATGTCCAAGAGGCCTGCAGTAATTATGTCCAAGAGGCCTGCAGTGTGACCAGGTATCGGGATGTTTGCATCCACTCGCTCTCACCGTTTTCGAGCTCAGCCAAGAACAGCCCGAGCAGGTGGGCCCGAGCCGGGGTTTCAGTTTCATTGAGCGAGGCCAAGAGTGCCACTCGGTACTTGGTAACTCTGAAAACGCACGGGGGTGGTTTGAAGGGGAGAAACGAAGTTGCTTTGTCCGACTGCATTGAGTGCTTTCAGAACGCGATCGACGAGCTTCACAAATCGCTCGATGTTCTGAGGACGCTGACTAAGATGACATTCGACACGAAAATGGCGGACCTGAATACCTGGCTCAGTGCTGCCCTAACCAATGGAGACACGTGCTTGGATGGCTTTGAGGGCCAGAATGGAAAACCAGTCAAATTGCTACAAGACAGGGTTTTGAAAGTTACCTATATCACCAGTAATGCACTGGCTCTTGTTAACAAACTTGCGACTACTGGCTTGGGAAGCCTTCCTAATTTGTAG

Protein Analysis

212

Amino Acids

22.94

Weight (kDa)

8.76

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 44 - 196 5.5e-38 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014556)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47670 AT3G62820
fragaria_vesca FvH4_2g25710
malus_domestica MD03G1288100.v1.1 MD11G1310200.v1.1
prunus_persica Prupe.8G261300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0293991
rosa_laevigata RLG00000011909
rosa_multiflora Rmu_sc0005126.1_g000006 Rmu_sc0005647.1_g000002
rosa_roxburghii Rroxscaffold_7G00173760
rosa_rugosa Rorug06G0239900
rosa_samantha Rh6AG352900 Rh6BG359900 Rh6CG367200 Rh6DG353600
rosa_wichuraiana Rw6G030750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 216
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 1 cut(s) 371
AciI CCGC 1 cut(s) 443
AcvI CACGTG 1 cut(s) 489
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 4 cut(s) 82, 165, 240, 241
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 3 cut(s) 72, 319, 553
AjnI CCWGG 2 cut(s) 158, 455
AleI CACNNNNGTG 1 cut(s) 309
AluBI AGCT 2 cut(s) 204, 382
AluI AGCT 2 cut(s) 204, 382
Alw21I GWGCWC 1 cut(s) 206
Alw26I GTCTC 1 cut(s) 477
Ama87I CYCGRG 2 cut(s) 220, 234
AoxI GGCC 5 cut(s) 120, 144, 231, 264, 508
ApaI GGGCCC 1 cut(s) 235
ApeKI GCWGC 1 cut(s) 467
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
AspS9I GGNCC 4 cut(s) 231, 232, 445, 508
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 2 cut(s) 183, 559
AvaI CYCGRG 2 cut(s) 220, 234
AvaII GGWCC 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 235
BanII GRGCYC 2 cut(s) 206, 235
BbrPI CACGTG 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 454
BccI CCATC 1 cut(s) 491
BciT130I CCWGG 2 cut(s) 160, 457
BcnI CCSGG 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 477
BfmI CTRYAG 2 cut(s) 124, 148
BfuAI ACCTGC 1 cut(s) 216
BisI GCNGC 1 cut(s) 468
BlpI GCTNAGC 1 cut(s) 205
BlsI GCNGC 1 cut(s) 469
Bme1390I CCNGG 3 cut(s) 160, 241, 457
Bme18I GGWCC 1 cut(s) 445
BmeT110I CYCGRG 2 cut(s) 220, 234
BmgT120I GGNCC 4 cut(s) 231, 232, 445, 508
BmiI GGNNCC 1 cut(s) 233
BmrFI CCNGG 3 cut(s) 160, 241, 457
BmsI GCATC 2 cut(s) 20, 186
BplI GAGNNNNNCTC 2 cut(s) 262, 294
Bpu1102I GCTNAGC 1 cut(s) 205
BpuMI CCSGG 1 cut(s) 241
BsaAI YACGTR 1 cut(s) 489
BsaJI CCNNGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 165, 240, 241
Bse1I ACTGG 4 cut(s) 524, 570, 585, 613
BseBI CCWGG 2 cut(s) 160, 457
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 3 cut(s) 175, 177, 502
BseLI CCNNNNNNNGG 4 cut(s) 82, 165, 240, 241
BseMII CTCAG 3 cut(s) 219, 395, 475
BseNI ACTGG 4 cut(s) 524, 570, 585, 613
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 1 cut(s) 454
Bsh1236I CGCG 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 5 cut(s) 122, 146, 233, 266, 510
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 206
BsiHKCI CYCGRG 2 cut(s) 220, 234
BsiSI CCGG 1 cut(s) 240
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 4 cut(s) 82, 165, 240, 241
BsmAI GTCTC 1 cut(s) 477
BsmFI GGGAC 1 cut(s) 113
BsnI GGCC 5 cut(s) 122, 146, 233, 266, 510
BsoBI CYCGRG 2 cut(s) 220, 234
Bsp120I GGGCCC 1 cut(s) 231
Bsp1286I GDGCHC 2 cut(s) 206, 235
Bsp143I GATC 1 cut(s) 372
Bsp1720I GCTNAGC 1 cut(s) 205
BspACI CCGC 1 cut(s) 443
BspANI GGCC 5 cut(s) 122, 146, 233, 266, 510
BspCNI CTCAG 3 cut(s) 218, 396, 474
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 1 cut(s) 233
BspMAI CTGCAG 2 cut(s) 128, 152
BspMI ACCTGC 1 cut(s) 216
BsrI ACTGG 4 cut(s) 524, 570, 585, 613
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 1 cut(s) 372
Bst2UI CCWGG 2 cut(s) 160, 457
Bst4CI ACNGT 1 cut(s) 195
BstAPI GCANNNNNTGC 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 2 cut(s) 124, 148
BstDEI CTNAG 4 cut(s) 205, 404, 417, 461
BstF5I GGATG 3 cut(s) 175, 177, 502
BstFNI CGCG 1 cut(s) 371
BstKTI GATC 1 cut(s) 375
BstMAI GTCTC 1 cut(s) 477
BstMBI GATC 1 cut(s) 372
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 5 cut(s) 272, 357, 498, 507, 618
BstNI CCWGG 2 cut(s) 160, 457
BstSCI CCNGG 3 cut(s) 158, 239, 455
BstSFI CTRYAG 2 cut(s) 124, 148
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 1 cut(s) 454
BsuRI GGCC 5 cut(s) 122, 146, 233, 266, 510
BtsCI GGATG 3 cut(s) 175, 177, 502
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 3 cut(s) 157, 469, 578
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 124, 148
Cfr13I GGNCC 4 cut(s) 231, 232, 445, 508
CseI GACGC 1 cut(s) 418
CsiI ACCWGGT 1 cut(s) 158
Csp6I GTAC 1 cut(s) 283
CviAII CATG 1 cut(s) 50
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 4 cut(s) 205, 404, 417, 461
DpnI GATC 1 cut(s) 374
DpnII GATC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 458
Ecl136II GAGCTC 1 cut(s) 204
Eco147I AGGCCT 2 cut(s) 122, 146
Eco24I GRGCYC 2 cut(s) 206, 235
Eco47I GGWCC 1 cut(s) 445
Eco53kI GAGCTC 1 cut(s) 204
Eco72I CACGTG 1 cut(s) 489
Eco88I CYCGRG 2 cut(s) 220, 234
EcoICRI GAGCTC 1 cut(s) 204
EcoRII CCWGG 2 cut(s) 158, 455
EcoT38I GRGCYC 2 cut(s) 206, 235
FaeI CATG 1 cut(s) 53
FaiI YATR 6 cut(s) 27, 51, 58, 111, 135, 564
FaqI GGGAC 1 cut(s) 113
FatI CATG 1 cut(s) 49
Fnu4HI GCNGC 1 cut(s) 468
FokI GGATG 3 cut(s) 164, 182, 509
FriOI GRGCYC 2 cut(s) 206, 235
Fsp4HI GCNGC 1 cut(s) 468
GluI GCNGC 1 cut(s) 468
HaeIII GGCC 5 cut(s) 122, 146, 233, 266, 510
HapII CCGG 1 cut(s) 240
HgaI GACGC 1 cut(s) 418
Hin1II CATG 1 cut(s) 53
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 1 cut(s) 240
HphI GGTGA 2 cut(s) 183, 559
Hpy166II GTNNAC 1 cut(s) 592
Hpy188I TCNGA 4 cut(s) 297, 346, 366, 405
Hpy188III TCNNGA 2 cut(s) 90, 167
Hpy8I GTNNAC 1 cut(s) 592
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 2 cut(s) 313, 632
HpyCH4III ACNGT 1 cut(s) 195
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 6 cut(s) 53, 126, 150, 177, 351, 578
HpyF10VI GCNNNNNNNGC 5 cut(s) 272, 357, 498, 507, 618
HpyF3I CTNAG 4 cut(s) 205, 404, 417, 461
HpySE526I ACGT 1 cut(s) 488
Hsp92II CATG 1 cut(s) 53
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 1 cut(s) 372
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 2 cut(s) 20, 186
MabI ACCWGGT 1 cut(s) 158
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 4 cut(s) 36, 154, 289, 556
MalI GATC 1 cut(s) 374
MboI GATC 1 cut(s) 372
MhlI GDGCHC 2 cut(s) 206, 235
MluCI AATT 4 cut(s) 77, 130, 530, 628
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 5 cut(s) 112, 136, 256, 399, 499
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 2 cut(s) 309, 437
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 3 cut(s) 160, 241, 457
MvaI CCWGG 2 cut(s) 160, 457
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 5 cut(s) 272, 357, 498, 507, 618
NciI CCSGG 1 cut(s) 241
NdeII GATC 1 cut(s) 372
NlaIII CATG 1 cut(s) 53
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 2 cut(s) 36, 154
OliI CACNNNNGTG 1 cut(s) 309
PaqCI CACCTGC 1 cut(s) 216
PceI AGGCCT 2 cut(s) 122, 146
PcsI WCGNNNNNNNCGW 2 cut(s) 191, 375
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 1 cut(s) 469
Ple19I CGATCG 1 cut(s) 375
PmaCI CACGTG 1 cut(s) 489
PmlI CACGTG 1 cut(s) 489
Ppu21I YACGTR 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 206
Psp6I CCWGG 2 cut(s) 158, 455
PspCI CACGTG 1 cut(s) 489
PspGI CCWGG 2 cut(s) 158, 455
PspN4I GGNNCC 1 cut(s) 233
PspOMI GGGCCC 1 cut(s) 231
PspPI GGNCC 4 cut(s) 231, 232, 445, 508
PstI CTGCAG 2 cut(s) 128, 152
PvuI CGATCG 1 cut(s) 375
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 2 cut(s) 309, 437
SacI GAGCTC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 1 cut(s) 468
Sau3AI GATC 1 cut(s) 372
Sau96I GGNCC 4 cut(s) 231, 232, 445, 508
ScrFI CCNGG 3 cut(s) 160, 241, 457
SduI GDGCHC 2 cut(s) 206, 235
SetI ASST 8 cut(s) 164, 206, 230, 384, 450, 458, 491, 563
SexAI ACCWGGT 1 cut(s) 158
SfaNI GCATC 2 cut(s) 20, 186
SfcI CTRYAG 2 cut(s) 124, 148
SinI GGWCC 1 cut(s) 445
SmiMI CAYNNNNRTG 2 cut(s) 309, 437
Sse9I AATT 4 cut(s) 77, 130, 530, 628
SseBI AGGCCT 2 cut(s) 122, 146
SsiI CCGC 1 cut(s) 443
SstI GAGCTC 1 cut(s) 206
StuI AGGCCT 2 cut(s) 122, 146
StyD4I CCNGG 3 cut(s) 158, 239, 455
TaaI ACNGT 1 cut(s) 195
TaiI ACGT 1 cut(s) 491
TaqI TCGA 4 cut(s) 200, 375, 396, 429
TasI AATT 4 cut(s) 77, 130, 530, 628
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 3 cut(s) 157, 469, 585
TseFI GTSAC 2 cut(s) 36, 154
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 2 cut(s) 36, 154
TspDTI ATGAA 3 cut(s) 17, 38, 243
TspRI CASTG 3 cut(s) 157, 469, 585
Van91I CCANNNNNTGG 1 cut(s) 82
VpaK11BI GGWCC 1 cut(s) 445
XcmI CCANNNNNNNNNTGG 2 cut(s) 283, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.