Rorug06G0239900

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014556
Physical Location & Seq
Reverse (-)
38241170 .. 38243929
2760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0239900.1

Sequence Viewer

Length: 1521 bp
ATGGGAGAAGTGGTTATTGAAACAAACAACATGGGAGATTTACATTTAGCACAAGCTATGGACCCTTCCTTTAACACCGCCATGGGTACAAGCTCACGGTCCGCTGCTCTAGTCATTCCCATGACAATTAGTTCCAGTGCAAACGAAGATGGTACAAATCCGGTAGGCGTAGGTGGTGAACTCGATAAGCAGGATGCTTGGCTGCCTCTCACAGAATCCAAGAATGGCAGTACCTATTCCGTGACGTTTCATCTACTTTGTTCAGGAATTGGGACTCAAGCTCTTTCACTCCCCGTTGCATTCGCCGCTCTTGGATGGGCATGGGGATGTATATGCTTGTTACTAGCGTTTGCATGGCAGCTCTACACTATACGGCTCCTTGTACTATCCCACGAATCAGATACCGGAACTCGTTATAGTAGATACCTCCAACTTGCTATTAGAGCCTTCGGTCAAAAACTAGGGAAGTTGCTAGTAATGTTCCCGATGATCTATCTATCAAATGGTGCTTGTGTCCAACTTATTATCGCAGGAGGTCTAACAATGGAGCTTTTCTTCAAAACCGTGTGTAACGATGGGGCTACTACTTATTGTCATCCAATGACTGGTACAGAGTGCTTCTTGGTGTTCATGATCATGGCTATTATTGTGGCTCAGTTTCCCAACTTGAACTCCATAGCTTGGGTCTCTTTGATTGGTTCTATCACAGCAATTGTATATTGCACTGTCATCTGGTCTATTTCAATTGCCAAGGGCAGACCTAATGGTATATCATACAATCCACCAGAAATGGAGTCTAATATGGATAGATTTGGTCGGATACTGAATGCGATTGGCATTGTGTTTTTGGCATTCAGAGGTCACAACGTCATTCTTGAAATACAGGGAACATTGCCATCAGAGCCAAAACACCCAATTCATAAGCGAATGTGGAGAGGGGTGATTATATCGTATGCTATCATTGCCATATGCTTGCTTCCTCTAGCCATAGCTGGATTTTGGGCACATGGAAACAAGGTGCCCTCCTCATCAGTTTTAGCAATAACTGCAATACTAAGCTCAGCTGCACAACTACTTGGACACAATAACACCTCAAGGTATGTTCTGGGAGTAATCTGCATTCTTGTAATAATACACTGCTTAAGCACATTTCAAATCTATGGCATGGTAGCCTTTGACAACTTGGAGATGAAGTACACTACGAGGAAGAACAAGCCGTGTGCAAGATGGCTCCGAGTAGCTTTCCGTTTTTTGTTTGGAGGATGGGTATTCTTTATAGCGGTGACCTTTCCGTTCGTTGTAAGCCTGGCACCTCTAATTGGAGGTTTGACGTTGCCTATGGCTTATGCTTATCCGTGTTTCATGTGGATCACACTCAAGAAACCGAAGCCGAAAGGTTTAATGTGGTGTATTAACGTGGGACTTGGATGCTTGGGACTGGTTTTGAGTGTTGTTTTAGTGGTTGCAGCAGCATGGAATTTAGCTCAGAAAGGTCTAAATGCCAATTTCTTCAAGCCCTAG

Protein Analysis

506

Amino Acids

55.2

Weight (kDa)

9.04

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 75 - 489 3.3e-53 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014556)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47670 AT3G62820
fragaria_vesca FvH4_2g25710
malus_domestica MD03G1288100.v1.1 MD11G1310200.v1.1
prunus_persica Prupe.8G261300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0293991
rosa_laevigata RLG00000011909
rosa_multiflora Rmu_sc0005126.1_g000006 Rmu_sc0005647.1_g000002
rosa_roxburghii Rroxscaffold_7G00173760
rosa_rugosa Rorug06G0239900
rosa_samantha Rh6AG352900 Rh6BG359900 Rh6CG367200 Rh6DG353600
rosa_wichuraiana Rw6G030750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1018, 1309
AccB7I CCANNNNNTGG 2 cut(s) 605, 681
AccBSI CCGCTC 1 cut(s) 308
AciI CCGC 4 cut(s) 78, 102, 306, 1280
AclWI GGATC 1 cut(s) 1376
AcsI RAATTY 1 cut(s) 1477
AfaI GTAC 6 cut(s) 88, 154, 232, 384, 610, 1196
AfiI CCNNNNNNNGG 3 cut(s) 605, 681, 1319
AflII CTTAAG 1 cut(s) 1141
AgsI TTSAA 7 cut(s) 20, 559, 670, 744, 878, 1154, 1513
AjnI CCWGG 1 cut(s) 1305
Alw26I GTCTC 1 cut(s) 691
AlwI GGATC 1 cut(s) 1376
ApeKI GCWGC 6 cut(s) 104, 202, 358, 1064, 1466, 1469
ApoI RAATTY 1 cut(s) 1477
AspS9I GGNCC 2 cut(s) 61, 99
AsuHPI GGTGA 3 cut(s) 188, 952, 1294
AvaII GGWCC 2 cut(s) 61, 99
BaeGI GKGCMC 2 cut(s) 1006, 1023
BanI GGYRCC 2 cut(s) 1018, 1309
BbvI GCAGC 6 cut(s) 91, 189, 370, 1051, 1478, 1481
BccI CCATC 6 cut(s) 143, 309, 569, 904, 1221, 1257
BceAI ACGGC 2 cut(s) 389, 1201
BciT130I CCWGG 1 cut(s) 1307
BciVI GTATCC 1 cut(s) 813
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 6 cut(s) 110, 344, 461, 473, 983, 1519
BfrI CTTAAG 1 cut(s) 1141
BfuI GTATCC 1 cut(s) 813
BisI GCNGC 7 cut(s) 105, 203, 306, 359, 1065, 1467, 1470
BlpI GCTNAGC 1 cut(s) 1060
BlsI GCNGC 7 cut(s) 106, 204, 307, 360, 1066, 1468, 1471
Bme1390I CCNGG 1 cut(s) 1307
Bme18I GGWCC 2 cut(s) 61, 99
BmgT120I GGNCC 2 cut(s) 61, 99
BmiI GGNNCC 5 cut(s) 63, 377, 1020, 1232, 1311
BmrFI CCNGG 1 cut(s) 1307
BmsI GCATC 2 cut(s) 184, 1418
Bpu1102I GCTNAGC 1 cut(s) 1060
BpuEI CTTGAG 3 cut(s) 261, 1078, 1361
BsaI GGTCTC 1 cut(s) 691
BsaJI CCNNGG 2 cut(s) 81, 750
BsaWI WCCGGW 2 cut(s) 160, 404
BsaXI ACNNNNNCTCC 7 cut(s) 27, 656, 686, 1178, 1208, 1251, 1281
Bsc4I CCNNNNNNNGG 3 cut(s) 605, 681, 1319
Bse1I ACTGG 3 cut(s) 135, 610, 1443
Bse3DI GCAATG 2 cut(s) 890, 960
BseBI CCWGG 1 cut(s) 1307
BseDI CCNNGG 2 cut(s) 81, 750
BseGI GGATG 6 cut(s) 199, 320, 332, 595, 1268, 1433
BseLI CCNNNNNNNGG 3 cut(s) 605, 681, 1319
BseMI GCAATG 2 cut(s) 890, 960
BseMII CTCAG 3 cut(s) 668, 1074, 1499
BseNI ACTGG 3 cut(s) 135, 610, 1443
BseRI GAGGAG 1 cut(s) 1015
BseSI GKGCMC 2 cut(s) 1006, 1023
BseXI GCAGC 6 cut(s) 91, 189, 370, 1051, 1478, 1481
BsgI GTGCAG 1 cut(s) 1050
BshNI GGYRCC 2 cut(s) 1018, 1309
BsiSI CCGG 2 cut(s) 161, 405
BslFI GGGAC 3 cut(s) 286, 1434, 1449
BslI CCNNNNNNNGG 3 cut(s) 605, 681, 1319
BsmAI GTCTC 1 cut(s) 691
BsmFI GGGAC 3 cut(s) 286, 1434, 1449
BsmI GAATGC 4 cut(s) 299, 832, 851, 1119
Bso31I GGTCTC 1 cut(s) 691
Bsp1286I GDGCHC 2 cut(s) 1006, 1023
Bsp143I GATC 3 cut(s) 489, 633, 1368
Bsp1720I GCTNAGC 1 cut(s) 1060
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 4 cut(s) 78, 102, 306, 1280
BspCNI CTCAG 3 cut(s) 667, 1073, 1498
BspHI TCATGA 1 cut(s) 630
BspLI GGNNCC 5 cut(s) 63, 377, 1020, 1232, 1311
BspPI GGATC 1 cut(s) 1376
BspT107I GGYRCC 2 cut(s) 1018, 1309
BspTI CTTAAG 1 cut(s) 1141
BspTNI GGTCTC 1 cut(s) 691
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 890, 960
BsrI ACTGG 3 cut(s) 135, 610, 1443
BssECI CCNNGG 2 cut(s) 81, 750
BssMI GATC 3 cut(s) 489, 633, 1368
BssT1I CCWWGG 2 cut(s) 81, 750
Bst2UI CCWGG 1 cut(s) 1307
Bst4CI ACNGT 3 cut(s) 99, 565, 727
BstAFI CTTAAG 1 cut(s) 1141
BstAPI GCANNNNNTGC 1 cut(s) 1046
BstC8I GCNNGC 1 cut(s) 974
BstDEI CTNAG 4 cut(s) 654, 1055, 1060, 1485
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 1 cut(s) 1282
BstF5I GGATG 6 cut(s) 199, 320, 332, 595, 1268, 1433
BstKTI GATC 3 cut(s) 492, 636, 1371
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 3 cut(s) 489, 633, 1368
BstMWI GCNNNNNNNGC 5 cut(s) 305, 443, 901, 962, 1046
BstNI CCWGG 1 cut(s) 1307
BstPI GGTNACC 1 cut(s) 1282
BstSCI CCNGG 1 cut(s) 1305
BstSLI GKGCMC 2 cut(s) 1006, 1023
BstV1I GCAGC 6 cut(s) 91, 189, 370, 1051, 1478, 1481
BsuI GTATCC 1 cut(s) 813
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 6 cut(s) 199, 320, 332, 595, 1268, 1433
BtsI GCAGTG 1 cut(s) 1135
BtsIMutI CAGTG 3 cut(s) 142, 723, 1135
Cac8I GCNNGC 1 cut(s) 974
CciI TCATGA 1 cut(s) 630
Cfr13I GGNCC 2 cut(s) 61, 99
CpoI CGGWCCG 1 cut(s) 99
Csp6I GTAC 6 cut(s) 87, 153, 231, 383, 609, 1195
CspI CGGWCCG 1 cut(s) 99
CviQI GTAC 6 cut(s) 87, 153, 231, 383, 609, 1195
DdeI CTNAG 4 cut(s) 654, 1055, 1060, 1485
DpnI GATC 3 cut(s) 491, 635, 1370
DpnII GATC 3 cut(s) 489, 633, 1368
Eco130I CCWWGG 2 cut(s) 81, 750
Eco31I GGTCTC 1 cut(s) 691
Eco47I GGWCC 2 cut(s) 61, 99
Eco91I GGTNACC 1 cut(s) 1282
EcoO65I GGTNACC 1 cut(s) 1282
EcoRII CCWGG 1 cut(s) 1305
EcoT14I CCWWGG 2 cut(s) 81, 750
ErhI CCWWGG 2 cut(s) 81, 750
FaqI GGGAC 3 cut(s) 286, 1434, 1449
FauNDI CATATG 1 cut(s) 968
FbaI TGATCA 1 cut(s) 633
Fnu4HI GCNGC 7 cut(s) 105, 203, 306, 359, 1065, 1467, 1470
FokI GGATG 6 cut(s) 206, 327, 339, 582, 1275, 1440
Fsp4HI GCNGC 7 cut(s) 105, 203, 306, 359, 1065, 1467, 1470
FspBI CTAG 6 cut(s) 110, 344, 461, 473, 983, 1519
GluI GCNGC 7 cut(s) 105, 203, 306, 359, 1065, 1467, 1470
HapII CCGG 2 cut(s) 161, 405
HinfI GANTC 4 cut(s) 215, 274, 395, 794
HpaII CCGG 2 cut(s) 161, 405
HphI GGTGA 3 cut(s) 188, 952, 1294
Hpy166II GTNNAC 2 cut(s) 179, 1197
Hpy188I TCNGA 6 cut(s) 400, 819, 857, 901, 1235, 1488
Hpy188III TCNNGA 5 cut(s) 264, 484, 631, 875, 1378
Hpy8I GTNNAC 2 cut(s) 179, 1197
HpyAV CCTTC 2 cut(s) 75, 457
HpyCH4III ACNGT 3 cut(s) 99, 565, 727
HpyCH4IV ACGT 4 cut(s) 245, 867, 1331, 1416
HpyCH4V TGCA 9 cut(s) 140, 299, 353, 723, 1049, 1067, 1119, 1223, 1466
HpyF10VI GCNNNNNNNGC 5 cut(s) 305, 443, 901, 962, 1046
HpyF3I CTNAG 4 cut(s) 654, 1055, 1060, 1485
HpySE526I ACGT 4 cut(s) 245, 867, 1331, 1416
Ksp22I TGATCA 1 cut(s) 633
Kzo9I GATC 3 cut(s) 489, 633, 1368
LmnI GCTCC 3 cut(s) 381, 547, 1236
Lsp1109I GCAGC 6 cut(s) 91, 189, 370, 1051, 1478, 1481
LweI GCATC 2 cut(s) 184, 1418
MaeI CTAG 6 cut(s) 110, 344, 461, 473, 983, 1519
MaeII ACGT 4 cut(s) 245, 867, 1331, 1416
MaeIII GTNAC 5 cut(s) 241, 339, 569, 860, 1282
MalI GATC 3 cut(s) 491, 635, 1370
MbiI CCGCTC 1 cut(s) 308
MboI GATC 3 cut(s) 489, 633, 1368
MboII GAAGA 4 cut(s) 158, 547, 1219, 1501
MfeI CAATTG 2 cut(s) 711, 744
MhlI GDGCHC 2 cut(s) 1006, 1023
MluCI AATT 8 cut(s) 126, 267, 711, 744, 915, 1317, 1477, 1504
MlyI GAGTC 2 cut(s) 268, 803
MmeI TCCRAC 3 cut(s) 454, 541, 797
MseI TTAA 4 cut(s) 72, 1142, 1400, 1413
MslI CAYNNNNRTG 4 cut(s) 80, 119, 325, 635
MspA1I CMGCKG 2 cut(s) 104, 1064
MspCI CTTAAG 1 cut(s) 1141
MspI CCGG 2 cut(s) 161, 405
MspR9I CCNGG 1 cut(s) 1307
MunI CAATTG 2 cut(s) 711, 744
Mva1269I GAATGC 4 cut(s) 299, 832, 851, 1119
MvaI CCWGG 1 cut(s) 1307
MwoI GCNNNNNNNGC 5 cut(s) 305, 443, 901, 962, 1046
NcoI CCATGG 1 cut(s) 81
NdeI CATATG 1 cut(s) 968
NdeII GATC 3 cut(s) 489, 633, 1368
NlaIV GGNNCC 5 cut(s) 63, 377, 1020, 1232, 1311
NmuCI GTSAC 3 cut(s) 241, 860, 1282
PagI TCATGA 1 cut(s) 630
PctI GAATGC 4 cut(s) 299, 832, 851, 1119
PfeI GAWTC 2 cut(s) 215, 395
PflMI CCANNNNNTGG 2 cut(s) 605, 681
PkrI GCNGC 7 cut(s) 106, 204, 307, 360, 1066, 1468, 1471
PleI GAGTC 2 cut(s) 268, 802
PpsI GAGTC 2 cut(s) 268, 802
Psp6I CCWGG 1 cut(s) 1305
PspEI GGTNACC 1 cut(s) 1282
PspGI CCWGG 1 cut(s) 1305
PspN4I GGNNCC 5 cut(s) 63, 377, 1020, 1232, 1311
PspPI GGNCC 2 cut(s) 61, 99
PvuII CAGCTG 1 cut(s) 1064
RsaI GTAC 6 cut(s) 88, 154, 232, 384, 610, 1196
RsaNI GTAC 6 cut(s) 87, 153, 231, 383, 609, 1195
RseI CAYNNNNRTG 4 cut(s) 80, 119, 325, 635
Rsr2I CGGWCCG 1 cut(s) 99
RsrII CGGWCCG 1 cut(s) 99
SaqAI TTAA 4 cut(s) 72, 1142, 1400, 1413
SatI GCNGC 7 cut(s) 105, 203, 306, 359, 1065, 1467, 1470
Sau3AI GATC 3 cut(s) 489, 633, 1368
Sau96I GGNCC 2 cut(s) 61, 99
SchI GAGTC 2 cut(s) 268, 803
ScrFI CCNGG 1 cut(s) 1307
SduI GDGCHC 2 cut(s) 1006, 1023
SfaNI GCATC 2 cut(s) 184, 1418
SinI GGWCC 2 cut(s) 61, 99
SmiMI CAYNNNNRTG 4 cut(s) 80, 119, 325, 635
SmlI CTYRAG 4 cut(s) 276, 1093, 1141, 1376
SmoI CTYRAG 4 cut(s) 276, 1093, 1141, 1376
Sse9I AATT 8 cut(s) 126, 267, 711, 744, 915, 1317, 1477, 1504
SsiI CCGC 4 cut(s) 78, 102, 306, 1280
SspMI CTAG 6 cut(s) 110, 344, 461, 473, 983, 1519
StyD4I CCNGG 1 cut(s) 1305
StyI CCWWGG 2 cut(s) 81, 750
TaaI ACNGT 3 cut(s) 99, 565, 727
TaiI ACGT 4 cut(s) 248, 870, 1334, 1419
TaqI TCGA 1 cut(s) 183
TaqII GACCGA 1 cut(s) 440
TasI AATT 8 cut(s) 126, 267, 711, 744, 915, 1317, 1477, 1504
TatI WGTACW 2 cut(s) 382, 1194
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 2 cut(s) 215, 395
Tru1I TTAA 4 cut(s) 72, 1142, 1400, 1413
Tru9I TTAA 4 cut(s) 72, 1142, 1400, 1413
TscAI CASTG 3 cut(s) 142, 730, 1142
TseFI GTSAC 3 cut(s) 241, 860, 1282
TseI GCWGC 6 cut(s) 104, 202, 358, 1064, 1466, 1469
Tsp45I GTSAC 3 cut(s) 241, 860, 1282
TspDTI ATGAA 5 cut(s) 239, 619, 908, 1205, 1351
TspGWI ACGGA 4 cut(s) 229, 1235, 1281, 1344
TspRI CASTG 3 cut(s) 142, 730, 1142
Van91I CCANNNNNTGG 2 cut(s) 605, 681
Vha464I CTTAAG 1 cut(s) 1141
VpaK11BI GGWCC 2 cut(s) 61, 99
XapI RAATTY 1 cut(s) 1477
XspI CTAG 6 cut(s) 110, 344, 461, 473, 983, 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.