FvH4_2g26620

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016762
Physical Location & Seq
Reverse (-)
21349075 .. 21350977
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26620.t1

Sequence Viewer

Length: 987 bp
ATGAAAGCTAATAACATCAGTCTGATCTTATTGGTCTGTATACTTGTTTGCATTAATCTAGGGGGTTGCTTAGGTGGTAGCCTGAGGAAAAATTTCTACAAGAAAACATGTCCTCGAGCAGAGCAGATTGTCAAGGACGCCACTTGGAACCACGTCTCCGCCAATCCCAACTTGCCAGCAAAGTTACTGAGGATGCATTTCCATGACTGTTTTGTTAGGGGATGTGACGCCTCGGTTTTGTTGAGCTCAACAGCAAATAACACTGCAGAGAGGGATTCGTTTCCAAACTTGTCTTTGAAGGGTTTTGATGTTATTGATGATATAAAAGAACAGTTGGAGAAGGCATGCCCAGGAGTTGTATCTTGTGCTGATATCCTCGCGTTGGCGACTAGGGATTCTGTGTCATTCCAATTCAAGAAATCACAATGGGATGTTCTTACTGGTAGACGAGATGGCAATGTGTCGATAGCAAATGAGGTCTCAGGCAACCTTCCAAGTCCTTTCTTCAACTTCGCCCAACTCAAACAAAGTTTTGCAAATAAAGGTCTCACTGTGCAAGATCTTGTGGTACTGTCAGGTGCACACAGCATTGGCGTTGGGCATTGCAATTTATTCAGCAAACGGCTCTACAACTTCACCGGAAAAGGAGATCAAGACCCGTCTATGGACTCCACATACGCTGCCTTGTTGAAGACTCAATGCCAAGGCCCTGGTGACAGAACCACAACTGTAGAATTAGACCCTGGAAGCTTCCAGAACTTTGACAATAGTTTCTACACAATCCTTAAGCAGAAGAAGGGTCTCTTCCAATCCGACGCTGCGCTTCTCACAAACAAAGTGTCAAGCAATGCAGTCCAGAAATTGCTCAAGGGAAATGATTTCTTCACGGAGTTTGGTCAGTCTATGAAGAGGATGGGAGCAATTGGAGTCCTTACAGGCACATCTGGGCAGATTAGGACGCAGTGTGGGTTTGTAAATTCCAACTAA

Protein Analysis

329

Amino Acids

35.69

Weight (kDa)

8.91

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 44 - 288 7.3e-83 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016762)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26620
malus_domestica MD03G1274400.v1.1 MD03G1274500.v1.1
prunus_persica Prupe.8G251100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0295471
rosa_laevigata RLG00000011798
rosa_multiflora Rmu_co8105696.1_g000001 Rmu_ssc0000464.1_g000001
rosa_rugosa Rorug06G0251200
rosa_samantha Rh6AG364400 Rh6BG372700 Rh6CG379000 Rh6DG365400
rosa_wichuraiana Rw6G031820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 40, 445
AccII CGCG 1 cut(s) 380
AciI CCGC 1 cut(s) 159
AcsI RAATTY 2 cut(s) 91, 976
AcyI GRCGYC 2 cut(s) 138, 228
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 2 cut(s) 382, 664
AflII CTTAAG 1 cut(s) 785
AflIII ACRYGT 1 cut(s) 107
AgsI TTSAA 4 cut(s) 298, 415, 508, 691
AjiI CACGTC 1 cut(s) 154
AjnI CCWGG 3 cut(s) 349, 709, 742
AloI GAACNNNNNNTCC 2 cut(s) 140, 172
AluBI AGCT 3 cut(s) 8, 246, 750
AluI AGCT 3 cut(s) 8, 246, 750
Alw21I GWGCWC 2 cut(s) 248, 583
Alw26I GTCTC 4 cut(s) 160, 484, 551, 806
Alw44I GTGCAC 1 cut(s) 579
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 1 cut(s) 706
ApaLI GTGCAC 1 cut(s) 579
ApeKI GCWGC 2 cut(s) 680, 818
ApoI RAATTY 2 cut(s) 91, 976
ArsI GACNNNNNNTTYG 2 cut(s) 824, 856
AseI ATTAAT 1 cut(s) 54
Asp700I GAANNNNTTC 1 cut(s) 92
AspLEI GCGC 1 cut(s) 823
AspS9I GGNCC 1 cut(s) 707
AsuHPI GGTGA 2 cut(s) 628, 725
AvaI CYCGRG 1 cut(s) 114
AxyI CCTNAGG 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 583
BanII GRGCYC 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 2 cut(s) 248, 583
BbvI GCAGC 2 cut(s) 667, 805
BccI CCATC 2 cut(s) 446, 907
BceAI ACGGC 1 cut(s) 638
BciT130I CCWGG 3 cut(s) 351, 711, 744
BcoDI GTCTC 4 cut(s) 160, 484, 551, 806
BfaI CTAG 2 cut(s) 59, 390
BfmI CTRYAG 2 cut(s) 264, 729
BfrI CTTAAG 1 cut(s) 785
BglII AGATCT 1 cut(s) 559
BisI GCNGC 2 cut(s) 681, 819
BlsI GCNGC 2 cut(s) 682, 820
Bme1390I CCNGG 3 cut(s) 351, 711, 744
BmeT110I CYCGRG 1 cut(s) 114
BmgBI CACGTC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 707
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 3 cut(s) 351, 711, 744
BmsI GCATC 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 698
Bpu10I CCTNAGC 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 851
BsaHI GRCGYC 2 cut(s) 138, 228
BsaI GGTCTC 3 cut(s) 484, 551, 806
BsaJI CCNNGG 5 cut(s) 231, 349, 703, 709, 742
BsaWI WCCGGW 1 cut(s) 638
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 664
Bse1I ACTGG 1 cut(s) 445
Bse21I CCTNAGG 1 cut(s) 83
Bse3DI GCAATG 3 cut(s) 463, 601, 853
BseBI CCWGG 3 cut(s) 351, 711, 744
BseDI CCNNGG 5 cut(s) 231, 349, 703, 709, 742
BseGI GGATG 4 cut(s) 198, 227, 436, 918
BseLI CCNNNNNNNGG 2 cut(s) 382, 664
BseMI GCAATG 3 cut(s) 463, 601, 853
BseMII CTCAG 3 cut(s) 74, 179, 495
BseNI ACTGG 1 cut(s) 445
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 2 cut(s) 667, 805
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 1 cut(s) 708
BsiHKAI GWGCWC 2 cut(s) 248, 583
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 1 cut(s) 639
BslI CCNNNNNNNGG 2 cut(s) 382, 664
BsmAI GTCTC 4 cut(s) 160, 484, 551, 806
BsmBI CGTCTC 1 cut(s) 160
BsnI GGCC 1 cut(s) 708
Bso31I GGTCTC 3 cut(s) 484, 551, 806
BsoBI CYCGRG 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 248, 583
Bsp143I GATC 3 cut(s) 24, 559, 649
BspACI CCGC 1 cut(s) 159
BspANI GGCC 1 cut(s) 708
BspCNI CTCAG 3 cut(s) 75, 180, 494
BspFNI CGCG 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 149
BspMAI CTGCAG 1 cut(s) 268
BspTI CTTAAG 1 cut(s) 785
BspTNI GGTCTC 3 cut(s) 484, 551, 806
BsrDI GCAATG 3 cut(s) 463, 601, 853
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 5 cut(s) 231, 349, 703, 709, 742
BssMI GATC 3 cut(s) 24, 559, 649
BssNAI GTATAC 1 cut(s) 41
BssNI GRCGYC 2 cut(s) 138, 228
BssT1I CCWWGG 1 cut(s) 703
Bst1107I GTATAC 1 cut(s) 41
Bst2UI CCWGG 3 cut(s) 351, 711, 744
Bst4CI ACNGT 5 cut(s) 209, 333, 553, 573, 730
Bst6I CTCTTC 2 cut(s) 809, 902
BstACI GRCGYC 2 cut(s) 138, 228
BstAFI CTTAAG 1 cut(s) 785
BstC8I GCNNGC 2 cut(s) 177, 346
BstDEI CTNAG 4 cut(s) 70, 83, 188, 481
BstF5I GGATG 4 cut(s) 198, 227, 436, 918
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 1 cut(s) 823
BstKTI GATC 3 cut(s) 27, 562, 652
BstMAI GTCTC 4 cut(s) 160, 484, 551, 806
BstMBI GATC 3 cut(s) 24, 559, 649
BstNI CCWGG 3 cut(s) 351, 711, 744
BstNSI RCATGY 2 cut(s) 111, 348
BstSCI CCNGG 3 cut(s) 349, 709, 742
BstSFI CTRYAG 2 cut(s) 264, 729
BstSLI GKGCMC 1 cut(s) 583
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 2 cut(s) 667, 805
BstV2I GAAGAC 1 cut(s) 698
BstX2I RGATCY 1 cut(s) 559
BstXI CCANNNNNNTGG 1 cut(s) 710
BstYI RGATCY 1 cut(s) 559
BstZ17I GTATAC 1 cut(s) 41
Bsu36I CCTNAGG 1 cut(s) 83
BsuRI GGCC 1 cut(s) 708
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 4 cut(s) 198, 227, 436, 918
BtsI GCAGTG 2 cut(s) 261, 968
BtsIMutI CAGTG 3 cut(s) 261, 549, 968
Cac8I GCNNGC 2 cut(s) 177, 346
CfoI GCGC 1 cut(s) 823
Cfr13I GGNCC 1 cut(s) 707
CseI GACGC 4 cut(s) 146, 236, 824, 967
Csp6I GTAC 1 cut(s) 569
CviAII CATG 3 cut(s) 108, 203, 345
CviJI RGCY 6 cut(s) 8, 81, 246, 625, 708, 750
CviKI_1 RGCY 6 cut(s) 8, 81, 246, 625, 708, 750
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 4 cut(s) 70, 83, 188, 481
DpnI GATC 3 cut(s) 26, 561, 651
DpnII GATC 3 cut(s) 24, 559, 649
Eam1104I CTCTTC 2 cut(s) 809, 902
EarI CTCTTC 2 cut(s) 809, 902
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 703
Eco24I GRGCYC 1 cut(s) 248
Eco31I GGTCTC 3 cut(s) 484, 551, 806
Eco32I GATATC 1 cut(s) 373
Eco53kI GAGCTC 1 cut(s) 246
Eco81I CCTNAGG 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 114
EcoICRI GAGCTC 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 707
EcoRII CCWGG 3 cut(s) 349, 709, 742
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 703
EcoT22I ATGCAT 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 703
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 3 cut(s) 111, 206, 348
FaiI YATR 8 cut(s) 41, 109, 204, 323, 346, 665, 676, 905
FalI AAGNNNNNCTT 2 cut(s) 788, 820
FatI CATG 3 cut(s) 107, 202, 344
FblI GTMKAC 2 cut(s) 40, 445
Fnu4HI GCNGC 2 cut(s) 681, 819
FokI GGATG 4 cut(s) 205, 234, 443, 925
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 2 cut(s) 681, 819
FspBI CTAG 2 cut(s) 59, 390
GlaI GCGC 1 cut(s) 822
GluI GCNGC 2 cut(s) 681, 819
HaeIII GGCC 1 cut(s) 708
HapII CCGG 1 cut(s) 639
HgaI GACGC 4 cut(s) 146, 236, 824, 967
HhaI GCGC 1 cut(s) 823
Hin1I GRCGYC 2 cut(s) 138, 228
Hin1II CATG 3 cut(s) 111, 206, 348
Hin6I GCGC 1 cut(s) 821
HinP1I GCGC 1 cut(s) 821
HindIII AAGCTT 1 cut(s) 748
HinfI GANTC 5 cut(s) 275, 395, 668, 694, 927
HpaII CCGG 1 cut(s) 639
HphI GGTGA 2 cut(s) 628, 725
Hpy166II GTNNAC 3 cut(s) 41, 446, 581
Hpy188I TCNGA 2 cut(s) 24, 814
Hpy188III TCNNGA 4 cut(s) 415, 653, 754, 856
Hpy8I GTNNAC 3 cut(s) 41, 446, 581
Hpy99I CGWCG 1 cut(s) 818
HpyAV CCTTC 4 cut(s) 292, 334, 500, 790
HpyCH4III ACNGT 5 cut(s) 209, 333, 553, 573, 730
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 8 cut(s) 51, 196, 266, 536, 556, 581, 606, 851
HpyF3I CTNAG 4 cut(s) 70, 83, 188, 481
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 2 cut(s) 138, 228
Hsp92II CATG 3 cut(s) 111, 206, 348
HspAI GCGC 1 cut(s) 821
Kzo9I GATC 3 cut(s) 24, 559, 649
LmnI GCTCC 1 cut(s) 917
Lsp1109I GCAGC 2 cut(s) 667, 805
LweI GCATC 1 cut(s) 183
MaeI CTAG 2 cut(s) 59, 390
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 3 cut(s) 183, 224, 713
MalI GATC 3 cut(s) 26, 561, 651
MboI GATC 3 cut(s) 24, 559, 649
MboII GAAGA 6 cut(s) 496, 703, 796, 805, 874, 919
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 2 cut(s) 248, 583
MluCI AATT 7 cut(s) 91, 410, 607, 734, 860, 921, 976
MlyI GAGTC 3 cut(s) 662, 688, 936
MmeI TCCRAC 2 cut(s) 315, 837
MnlI CCTC 8 cut(s) 78, 123, 183, 241, 264, 386, 469, 903
Mph1103I ATGCAT 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 2 cut(s) 54, 786
MslI CAYNNNNRTG 1 cut(s) 201
MspCI CTTAAG 1 cut(s) 785
MspI CCGG 1 cut(s) 639
MspR9I CCNGG 3 cut(s) 351, 711, 744
MunI CAATTG 1 cut(s) 921
MvaI CCWGG 3 cut(s) 351, 711, 744
MvnI CGCG 1 cut(s) 380
NdeII GATC 3 cut(s) 24, 559, 649
NlaIII CATG 3 cut(s) 111, 206, 348
NlaIV GGNNCC 1 cut(s) 149
NmuCI GTSAC 2 cut(s) 224, 713
NsiI ATGCAT 1 cut(s) 198
NspI RCATGY 2 cut(s) 111, 348
PaeI GCATGC 1 cut(s) 348
PaeR7I CTCGAG 1 cut(s) 114
PciI ACATGT 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 2 cut(s) 275, 395
PkrI GCNGC 2 cut(s) 682, 820
PleI GAGTC 3 cut(s) 662, 688, 935
PpsI GAGTC 3 cut(s) 662, 688, 935
PscI ACATGT 1 cut(s) 107
PshBI ATTAAT 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 248
Psp6I CCWGG 3 cut(s) 349, 709, 742
PspGI CCWGG 3 cut(s) 349, 709, 742
PspN4I GGNNCC 1 cut(s) 149
PspPI GGNCC 1 cut(s) 707
PspXI VCTCGAGB 1 cut(s) 114
PstI CTGCAG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 1 cut(s) 201
SacI GAGCTC 1 cut(s) 248
SaqAI TTAA 2 cut(s) 54, 786
SatI GCNGC 2 cut(s) 681, 819
Sau3AI GATC 3 cut(s) 24, 559, 649
Sau96I GGNCC 1 cut(s) 707
SchI GAGTC 3 cut(s) 662, 688, 936
ScrFI CCNGG 3 cut(s) 351, 711, 744
SduI GDGCHC 2 cut(s) 248, 583
SetI ASST 9 cut(s) 10, 76, 156, 248, 480, 492, 547, 580, 752
SfaNI GCATC 1 cut(s) 183
SfcI CTRYAG 2 cut(s) 264, 729
Sfr274I CTCGAG 1 cut(s) 114
SlaI CTCGAG 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 201
SmlI CTYRAG 3 cut(s) 114, 785, 866
SmoI CTYRAG 3 cut(s) 114, 785, 866
SphI GCATGC 1 cut(s) 348
Sse9I AATT 7 cut(s) 91, 410, 607, 734, 860, 921, 976
SsiI CCGC 1 cut(s) 159
SspMI CTAG 2 cut(s) 59, 390
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 3 cut(s) 349, 709, 742
StyI CCWWGG 1 cut(s) 703
TaaI ACNGT 5 cut(s) 209, 333, 553, 573, 730
TaiI ACGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 115, 464
TasI AATT 7 cut(s) 91, 410, 607, 734, 860, 921, 976
TfiI GAWTC 2 cut(s) 275, 395
Tru1I TTAA 2 cut(s) 54, 786
Tru9I TTAA 2 cut(s) 54, 786
TscAI CASTG 3 cut(s) 268, 556, 968
TseFI GTSAC 2 cut(s) 224, 713
TseI GCWGC 2 cut(s) 680, 818
Tsp45I GTSAC 2 cut(s) 224, 713
TspDTI ATGAA 2 cut(s) 17, 920
TspGWI ACGGA 1 cut(s) 902
TspRI CASTG 3 cut(s) 268, 556, 968
Vha464I CTTAAG 1 cut(s) 785
VneI GTGCAC 1 cut(s) 579
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 2 cut(s) 91, 976
XceI RCATGY 2 cut(s) 111, 348
XhoI CTCGAG 1 cut(s) 114
XmiI GTMKAC 2 cut(s) 40, 445
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 2 cut(s) 59, 390
Zsp2I ATGCAT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.