FvH4_2g27330

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0018382
Physical Location & Seq
Reverse (-)
21723478 .. 21725171
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27330.t1

Sequence Viewer

Length: 1146 bp
ATGGCGGTGGCAACAATCGGAGCGGATTCGCTTGGACCAGTCTTTCAAGAACTCTGCGATCAAGTTAGCCGATCGATGGAGAAGAATAGCAAGTTCAAAACGCTCTTGGGTTACTTGCAGTGCACCCTGAACTCCGTGAAAGAACTAATCATCCAACCTATGCAAGAAAACCTACAACTACAAGAAGAGACTATGCGAGAGGAGCGAACTGATGAACGTTTGATGGTAATAGTGCAGGAGGGCCAAAAGCTAATAACTATTAAGTTCTCCAAGCTCCGACGTTGGGACCTCTATTTCTGCATGGATGATCACACCCTCCGACTTCTCGAATTGGATAGTTTCCTCCGATATCTCTTGGAGGCGTTGTGGGTAAGGGAATTGGATGATGTGAATGACATCAAAGGCTATATGCAAAACTCGATGAGCGTCGGAGTAGTGTACAACTTTTTGAATGAAAATCTTCAGAGAATTGTAGATACTTTTGAGAAGGCATTGGAGCCGGAAACTATGGCTCCGGAGGAGAGAAATCATGAAGAGGAAGAAGGAATGGAACAACCGGCGCTGTTGGTGTTTGAGTCATTGTCTTTCAAGGTCCATGAAGTGTTGAACAAGAAGAAGACACCTGTGATGTTTAAATTGCTACTTAATGATATTTTTGACACACTCTCATTGTTGAAACCCTTCATGGAAGATAAGGCAGAAGCATTTGATGGACAAGACGAGGAACTAAGAATTTTCAAAGCAAAGATGGTGAAGGGGGTAGAGTTTGTTCGCAAGAGCTCCGAGTTTGGTGTGTGGGGTGGCTACAGTAAGAGAAAGTATGAGTGCATCAACAACCTACTCGGACTGAATCATTCTCTCAGAAGACTATTATGTATAACGAGATGGGTGGAGGCAAGGAAGGCGAGGAATGAATTGCATTTCCGGTTGAGTACGGCTTTCAAGGAACTCAAAGACAGTTACCTGGTGCAGAACCAAACCGAGAGTAGTGCTGATGGCAAAGAATGCGATGAGGAAGGTGTAACTGATAAGAGGAAGGCCTTGGCTTTCAGGAGGATGGTGGAGACCGTGGAGATGGCTAGTGATGAATTCAAATGCATTACTGGTGCGGAGCAGAATCAAACTCGTTTTGCTGGTCTAATGTGA

Protein Analysis

382

Amino Acids

44.38

Weight (kDa)

5.22

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 10 - 122 6.7e-07 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 199 - 307 8.5e-08 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018382)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g27330
rosa_laevigata RLG00000011723
rosa_multiflora Rmu_sc0000486.1_g000007
rosa_roxburghii Rroxscaffold_7G00178260
rosa_rugosa Rorug06G0258800
rosa_samantha Rh6AG371100 Rh6BG379000 Rh6CG384300 Rh6DG371600 Rh7CG403600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 514
AciI CCGC 3 cut(s) 5, 23, 1109
AclI AACGTT 1 cut(s) 217
AcsI RAATTY 2 cut(s) 732, 1088
AcuI CTGAAG 1 cut(s) 446
AfaI GTAC 2 cut(s) 440, 934
AfiI CCNNNNNNNGG 2 cut(s) 76, 283
AgsI TTSAA 9 cut(s) 47, 97, 451, 589, 607, 676, 739, 943, 1093
AjnI CCWGG 1 cut(s) 963
AloI GAACNNNNNNTCC 2 cut(s) 135, 167
AluBI AGCT 3 cut(s) 250, 274, 780
AluI AGCT 3 cut(s) 250, 274, 780
Alw21I GWGCWC 2 cut(s) 125, 782
Alw26I GTCTC 2 cut(s) 182, 1058
Alw44I GTGCAC 1 cut(s) 121
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 2 cut(s) 241, 1038
ApaLI GTGCAC 1 cut(s) 121
ApoI RAATTY 2 cut(s) 732, 1088
Asp700I GAANNNNTTC 2 cut(s) 459, 680
AspLEI GCGC 1 cut(s) 562
AspS9I GGNCC 4 cut(s) 35, 241, 286, 592
AsuHPI GGTGA 1 cut(s) 763
AvaII GGWCC 3 cut(s) 35, 286, 592
BaeGI GKGCMC 1 cut(s) 125
BanII GRGCYC 1 cut(s) 782
BbsI GAAGAC 2 cut(s) 623, 871
Bbv12I GWGCWC 2 cut(s) 125, 782
BccI CCATC 8 cut(s) 70, 217, 704, 742, 879, 989, 1051, 1069
BceAI ACGGC 1 cut(s) 951
BcgI CGANNNNNNTGC 2 cut(s) 971, 1005
BciT130I CCWGG 1 cut(s) 965
BclI TGATCA 1 cut(s) 307
BcoDI GTCTC 2 cut(s) 182, 1058
BfaI CTAG 1 cut(s) 1080
BfmI CTRYAG 1 cut(s) 805
BfoI RGCGCY 1 cut(s) 563
Bme1390I CCNGG 1 cut(s) 965
Bme18I GGWCC 3 cut(s) 35, 286, 592
BmgT120I GGNCC 4 cut(s) 35, 241, 286, 592
BmiI GGNNCC 3 cut(s) 287, 498, 513
BmrFI CCNGG 1 cut(s) 965
BmsI GCATC 1 cut(s) 837
BpiI GAAGAC 2 cut(s) 623, 871
Bsa29I ATCGAT 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 1058
BsaJI CCNNGG 2 cut(s) 1041, 1068
BsaWI WCCGGW 2 cut(s) 514, 924
BsaXI ACNNNNNCTCC 4 cut(s) 350, 380, 496, 526
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 283
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 2 cut(s) 38, 1108
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 1 cut(s) 965
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 2 cut(s) 1041, 1068
BseGI GGATG 4 cut(s) 150, 310, 388, 1062
BseLI CCNNNNNNNGG 2 cut(s) 76, 283
BseMII CTCAG 1 cut(s) 874
BseNI ACTGG 2 cut(s) 38, 1108
BseRI GAGGAG 2 cut(s) 215, 533
BseSI GKGCMC 1 cut(s) 125
BsgI GTGCAG 2 cut(s) 254, 989
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 2 cut(s) 243, 1040
BshVI ATCGAT 1 cut(s) 74
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 2 cut(s) 125, 782
BsiSI CCGG 4 cut(s) 500, 515, 557, 925
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 2 cut(s) 76, 283
BsmAI GTCTC 2 cut(s) 182, 1058
BsmFI GGGAC 1 cut(s) 299
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 2 cut(s) 243, 1040
Bso31I GGTCTC 1 cut(s) 1058
Bsp1286I GDGCHC 2 cut(s) 125, 782
Bsp13I TCCGGA 1 cut(s) 514
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 3 cut(s) 58, 71, 307
BspACI CCGC 3 cut(s) 5, 23, 1109
BspANI GGCC 2 cut(s) 243, 1040
BspCNI CTCAG 1 cut(s) 873
BspDI ATCGAT 1 cut(s) 74
BspEI TCCGGA 1 cut(s) 514
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 287, 498, 513
BspTNI GGTCTC 1 cut(s) 1058
BsrBI CCGCTC 1 cut(s) 23
BsrFI RCCGGY 1 cut(s) 556
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 2 cut(s) 38, 1108
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 2 cut(s) 1041, 1068
BssMI GATC 3 cut(s) 58, 71, 307
BssT1I CCWWGG 1 cut(s) 1041
Bst2UI CCWGG 1 cut(s) 965
Bst4CI ACNGT 3 cut(s) 809, 959, 1069
Bst6I CTCTTC 2 cut(s) 180, 528
BstAPI GCANNNNNTGC 1 cut(s) 1005
BstAUI TGTACA 1 cut(s) 438
BstDEI CTNAG 2 cut(s) 728, 860
BstDSI CCRYGG 1 cut(s) 1068
BstF5I GGATG 4 cut(s) 150, 310, 388, 1062
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 1 cut(s) 562
BstKTI GATC 3 cut(s) 61, 74, 310
BstMAI GTCTC 2 cut(s) 182, 1058
BstMBI GATC 3 cut(s) 58, 71, 307
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 3 cut(s) 202, 902, 1005
BstNI CCWGG 1 cut(s) 965
BstSCI CCNGG 1 cut(s) 963
BstSFI CTRYAG 1 cut(s) 805
BstSLI GKGCMC 1 cut(s) 125
BstV2I GAAGAC 2 cut(s) 623, 871
Bsu15I ATCGAT 1 cut(s) 74
BsuRI GGCC 2 cut(s) 243, 1040
BsuTUI ATCGAT 1 cut(s) 74
BtgI CCRYGG 1 cut(s) 1068
BtgZI GCGATG 1 cut(s) 1023
BtsCI GGATG 4 cut(s) 150, 310, 388, 1062
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 125
CciI TCATGA 1 cut(s) 529
CfoI GCGC 1 cut(s) 562
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 4 cut(s) 35, 241, 286, 592
ClaI ATCGAT 1 cut(s) 74
CseI GACGC 1 cut(s) 415
CsiI ACCWGGT 1 cut(s) 963
Csp6I GTAC 2 cut(s) 439, 933
CviAII CATG 4 cut(s) 301, 530, 596, 685
CviQI GTAC 2 cut(s) 439, 933
DdeI CTNAG 2 cut(s) 728, 860
DpnI GATC 3 cut(s) 60, 73, 309
DpnII GATC 3 cut(s) 58, 71, 307
DraI TTTAAA 1 cut(s) 634
Eam1104I CTCTTC 2 cut(s) 180, 528
EarI CTCTTC 2 cut(s) 180, 528
Ecl136II GAGCTC 1 cut(s) 780
Eco130I CCWWGG 1 cut(s) 1041
Eco147I AGGCCT 1 cut(s) 1040
Eco24I GRGCYC 1 cut(s) 782
Eco31I GGTCTC 1 cut(s) 1058
Eco32I GATATC 1 cut(s) 350
Eco47I GGWCC 3 cut(s) 35, 286, 592
Eco53kI GAGCTC 1 cut(s) 780
Eco57I CTGAAG 1 cut(s) 446
EcoICRI GAGCTC 1 cut(s) 780
EcoO109I RGGNCCY 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 1088
EcoRII CCWGG 1 cut(s) 963
EcoRV GATATC 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 1041
EcoT22I ATGCAT 1 cut(s) 1100
EcoT38I GRGCYC 1 cut(s) 782
ErhI CCWWGG 1 cut(s) 1041
FaeI CATG 4 cut(s) 304, 533, 599, 688
FaqI GGGAC 1 cut(s) 299
FatI CATG 4 cut(s) 300, 529, 595, 684
FbaI TGATCA 1 cut(s) 307
FokI GGATG 4 cut(s) 137, 317, 395, 1069
FriOI GRGCYC 1 cut(s) 782
FspBI CTAG 1 cut(s) 1080
GlaI GCGC 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 2 cut(s) 243, 1040
HapII CCGG 4 cut(s) 500, 515, 557, 925
HgaI GACGC 1 cut(s) 415
HhaI GCGC 1 cut(s) 562
Hin1II CATG 4 cut(s) 304, 533, 599, 688
Hin6I GCGC 1 cut(s) 560
HinP1I GCGC 1 cut(s) 560
HinfI GANTC 4 cut(s) 26, 575, 850, 1117
HpaII CCGG 4 cut(s) 500, 515, 557, 925
HphI GGTGA 1 cut(s) 763
Hpy166II GTNNAC 2 cut(s) 123, 439
Hpy188I TCNGA 9 cut(s) 20, 278, 320, 347, 431, 465, 784, 845, 863
Hpy188III TCNNGA 5 cut(s) 47, 326, 515, 530, 1051
Hpy8I GTNNAC 2 cut(s) 123, 439
Hpy99I CGWCG 2 cut(s) 282, 431
HpyAV CCTTC 7 cut(s) 481, 536, 691, 748, 895, 1010, 1030
HpyCH4III ACNGT 3 cut(s) 809, 959, 1069
HpyCH4IV ACGT 2 cut(s) 217, 280
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 902, 1005
HpyF3I CTNAG 2 cut(s) 728, 860
HpySE526I ACGT 2 cut(s) 217, 280
Hsp92II CATG 4 cut(s) 304, 533, 599, 688
HspAI GCGC 1 cut(s) 560
Kpn2I TCCGGA 1 cut(s) 514
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 3 cut(s) 58, 71, 307
LmnI GCTCC 7 cut(s) 20, 202, 279, 496, 517, 785, 1111
LweI GCATC 1 cut(s) 837
MabI ACCWGGT 1 cut(s) 963
MaeI CTAG 1 cut(s) 1080
MaeII ACGT 2 cut(s) 217, 280
MaeIII GTNAC 3 cut(s) 110, 959, 1021
MalI GATC 3 cut(s) 60, 73, 309
MbiI CCGCTC 1 cut(s) 23
MboI GATC 3 cut(s) 58, 71, 307
MboII GAAGA 9 cut(s) 94, 197, 452, 545, 551, 625, 628, 701, 876
MhlI GDGCHC 2 cut(s) 125, 782
MluCI AATT 7 cut(s) 329, 377, 468, 635, 732, 914, 1088
MlyI GAGTC 1 cut(s) 584
MmeI TCCRAC 4 cut(s) 178, 301, 343, 409
Mph1103I ATGCAT 1 cut(s) 1100
MroI TCCGGA 1 cut(s) 514
MroXI GAANNNNTTC 2 cut(s) 459, 680
MseI TTAA 3 cut(s) 261, 633, 645
MspI CCGG 4 cut(s) 500, 515, 557, 925
MspR9I CCNGG 1 cut(s) 965
Mva1269I GAATGC 1 cut(s) 1010
MvaI CCWGG 1 cut(s) 965
MwoI GCNNNNNNNGC 3 cut(s) 202, 902, 1005
NdeII GATC 3 cut(s) 58, 71, 307
NlaIII CATG 4 cut(s) 304, 533, 599, 688
NlaIV GGNNCC 3 cut(s) 287, 498, 513
NsiI ATGCAT 1 cut(s) 1100
PagI TCATGA 1 cut(s) 529
PceI AGGCCT 1 cut(s) 1040
PctI GAATGC 1 cut(s) 1010
PdmI GAANNNNTTC 2 cut(s) 459, 680
PfeI GAWTC 3 cut(s) 26, 850, 1117
Ple19I CGATCG 1 cut(s) 74
PleI GAGTC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 583
PpuMI RGGWCCY 1 cut(s) 286
Psp124BI GAGCTC 1 cut(s) 782
Psp1406I AACGTT 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 286
Psp6I CCWGG 1 cut(s) 963
PspGI CCWGG 1 cut(s) 963
PspN4I GGNNCC 3 cut(s) 287, 498, 513
PspPI GGNCC 4 cut(s) 35, 241, 286, 592
PspPPI RGGWCCY 1 cut(s) 286
PsrI GAACNNNNNNTAC 2 cut(s) 753, 785
PvuI CGATCG 1 cut(s) 74
RsaI GTAC 2 cut(s) 440, 934
RsaNI GTAC 2 cut(s) 439, 933
SacI GAGCTC 1 cut(s) 782
SaqAI TTAA 3 cut(s) 261, 633, 645
Sau3AI GATC 3 cut(s) 58, 71, 307
Sau96I GGNCC 4 cut(s) 35, 241, 286, 592
SchI GAGTC 1 cut(s) 584
ScrFI CCNGG 1 cut(s) 965
SduI GDGCHC 2 cut(s) 125, 782
SexAI ACCWGGT 1 cut(s) 963
SfaNI GCATC 1 cut(s) 837
SfcI CTRYAG 1 cut(s) 805
SinI GGWCC 3 cut(s) 35, 286, 592
Sse9I AATT 7 cut(s) 329, 377, 468, 635, 732, 914, 1088
SseBI AGGCCT 1 cut(s) 1040
SsiI CCGC 3 cut(s) 5, 23, 1109
SspMI CTAG 1 cut(s) 1080
SstI GAGCTC 1 cut(s) 782
StuI AGGCCT 1 cut(s) 1040
StyD4I CCNGG 1 cut(s) 963
StyI CCWWGG 1 cut(s) 1041
TaaI ACNGT 3 cut(s) 809, 959, 1069
TaiI ACGT 2 cut(s) 220, 283
TaqI TCGA 3 cut(s) 74, 327, 419
TasI AATT 7 cut(s) 329, 377, 468, 635, 732, 914, 1088
TatI WGTACW 1 cut(s) 438
TfiI GAWTC 3 cut(s) 26, 850, 1117
Tru1I TTAA 3 cut(s) 261, 633, 645
Tru9I TTAA 3 cut(s) 261, 633, 645
TscAI CASTG 1 cut(s) 125
TspDTI ATGAA 7 cut(s) 228, 468, 546, 612, 673, 927, 1101
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 1 cut(s) 125
VneI GTGCAC 1 cut(s) 121
VpaK11BI GGWCC 3 cut(s) 35, 286, 592
XapI RAATTY 2 cut(s) 732, 1088
XmnI GAANNNNTTC 2 cut(s) 459, 680
XspI CTAG 1 cut(s) 1080
Zsp2I ATGCAT 1 cut(s) 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.