Rroxscaffold_7G00178260

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0018382
Physical Location & Seq
Reverse (-)
18003179 .. 18004336
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00178260.1

Sequence Viewer

Length: 1158 bp
ATGGCGGTTGCAACAATCGGAACGGCTTCGCTTGGACCAGTCTTTCAAGAACTCTGCGATCAAGTTACCCAAGCGATGGAGAAGAATAGCAAGTTCAAAACTCTCTTGGGCTACTTGCAGTGCTCGCTCAACTCCGTGAAAGAACGAATCATCCAACCTATAGAAGAAAAGCCCGAGAATTTCCGAGATGAAGGAATTGAAAGTTTGATGATACTAGTGCAGAAGGGCCTAAAGCTAATAACTGAGAAGTTCTCCAAGCTCCGACGTTGGGACCTCTATTTCTGCATGGATGATCACACCGTCGAACTTCTCGATTTGGATAGTTTTCTCAGAGAGCTGATGGAGCAGTTTTGGGTAGGGGAGTTACGTGTTGTGAATGGCATCAGAGGCTATATGCAATACTCGATGAGCCCCGAAGAGTACAACATGTTGAATGAAAATCTTCAGAGAATCGTAGATACTTTTGAGAAGGCATGGGAGTCAGATACCATGGCTCCGGAGGAGCCAAATCATGAAGAGGAAGAAGGAATGGAACGAGGGGCGCTGTTGGTGTTTGAGTCATTGTTTGAAACGGTCCATGAAGTGTTGAACAAGAAGAAGACGCCTGTGATGTTTAAATTGCTGCTTGGCGATATTTTTGACAGACTCTTATTGTTGAAACCATTTATGGAAAAGAAGGCAGAAGCATTTGATGGCACAGACGAGGAACTAAGAAATTTCAAAGCAAAGATGGTGAAAGGGGTGGAGATTGTTCGCAAGAGCTCTGGGTTTGGTATGTGGGGTGGCTACAATAAGAGAAAGTATGAGTGCATCAACAACCTACTCGGACTGAATCAATCTCTTGAAAGACTATTATGTATAACGAGATGGGTGGAGGCAAGGAAGGCGAGGCATGCCTTGCTTTTCCTGAAATATTTGAAAACGGCTTCCAATGAATTAAAAAGCAGTTATCTGGTCCAGAATCAAACTGAGAGAGCTGATGGCAAAGAATGCGAGGAAGGCCTAATTGAAAGCAGTGTTGTGGTGCAGGATCAAGCTAGATCGAGGAAGGCCTTGGCTTTTAGGATGATGGTGGAGACGGTTAATGAATTTAAATGCAGTGGTACGTGTGGACGGTGTGGTGCAGAATCAAACTCTCGGTTTGCTGATTTAATGTGA

Protein Analysis

385

Amino Acids

44.42

Weight (kDa)

5.43

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 92 7.1e-07 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018382)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g27330
rosa_laevigata RLG00000011723
rosa_multiflora Rmu_sc0000486.1_g000007
rosa_roxburghii Rroxscaffold_7G00178260
rosa_rugosa Rorug06G0258800
rosa_samantha Rh6AG371100 Rh6BG379000 Rh6CG384300 Rh6DG371600 Rh7CG403600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 76
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 1038
AcsI RAATTY 3 cut(s) 178, 715, 1088
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 602
AfaI GTAC 2 cut(s) 422, 1105
AfiI CCNNNNNNNGG 2 cut(s) 76, 268
AflIII ACRYGT 3 cut(s) 367, 426, 1106
AhlI ACTAGT 1 cut(s) 214
AloI GAACNNNNNNTCC 2 cut(s) 135, 167
AluBI AGCT 6 cut(s) 235, 259, 337, 762, 977, 1037
AluI AGCT 6 cut(s) 235, 259, 337, 762, 977, 1037
Alw21I GWGCWC 2 cut(s) 125, 764
Alw26I GTCTC 1 cut(s) 1070
AlwI GGATC 1 cut(s) 1038
Ama87I CYCGRG 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 3 cut(s) 226, 1000, 1050
ApeKI GCWGC 1 cut(s) 622
ApoI RAATTY 3 cut(s) 178, 715, 1088
Asp700I GAANNNNTTC 2 cut(s) 25, 441
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 5 cut(s) 35, 226, 271, 574, 955
AsuHPI GGTGA 1 cut(s) 745
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 4 cut(s) 35, 271, 574, 955
BanII GRGCYC 2 cut(s) 413, 764
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 2 cut(s) 125, 764
BbvI GCAGC 1 cut(s) 609
BccI CCATC 7 cut(s) 70, 334, 686, 724, 861, 974, 1063
BceAI ACGGC 2 cut(s) 39, 939
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 1070
BcuI ACTAGT 1 cut(s) 214
BfaI CTAG 2 cut(s) 215, 1038
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 545
BisI GCNGC 1 cut(s) 623
BlsI GCNGC 1 cut(s) 624
Bme18I GGWCC 4 cut(s) 35, 271, 574, 955
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 5 cut(s) 35, 226, 271, 574, 955
BmiI GGNNCC 3 cut(s) 272, 495, 504
BmsI GCATC 2 cut(s) 390, 819
BpiI GAAGAC 1 cut(s) 605
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BsaAI YACGTR 2 cut(s) 368, 1107
BsaHI GRCGYC 1 cut(s) 602
BsaJI CCNNGG 2 cut(s) 489, 1053
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 268
Bse1I ACTGG 1 cut(s) 38
BseAI TCCGGA 1 cut(s) 496
BseDI CCNNGG 2 cut(s) 489, 1053
BseGI GGATG 3 cut(s) 150, 295, 1071
BseLI CCNNNNNNNGG 2 cut(s) 76, 268
BseMII CTCAG 3 cut(s) 234, 343, 960
BseNI ACTGG 1 cut(s) 38
BseRI GAGGAG 1 cut(s) 515
BseXI GCAGC 1 cut(s) 609
BsgI GTGCAG 3 cut(s) 239, 1046, 1143
BshFI GGCC 3 cut(s) 228, 1002, 1052
BsiHKAI GWGCWC 2 cut(s) 125, 764
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 1 cut(s) 497
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 2 cut(s) 76, 268
BsmAI GTCTC 1 cut(s) 1070
BsmBI CGTCTC 1 cut(s) 1070
BsmFI GGGAC 1 cut(s) 284
BsmI GAATGC 1 cut(s) 995
BsnI GGCC 3 cut(s) 228, 1002, 1052
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 3 cut(s) 125, 413, 764
Bsp13I TCCGGA 1 cut(s) 496
Bsp143I GATC 4 cut(s) 58, 292, 1030, 1040
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 1 cut(s) 5
BspANI GGCC 3 cut(s) 228, 1002, 1052
BspCNI CTCAG 3 cut(s) 235, 342, 961
BspEI TCCGGA 1 cut(s) 496
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 272, 495, 504
BspPI GGATC 1 cut(s) 1038
BsrI ACTGG 1 cut(s) 38
BssECI CCNNGG 2 cut(s) 489, 1053
BssMI GATC 4 cut(s) 58, 292, 1030, 1040
BssNI GRCGYC 1 cut(s) 602
BssT1I CCWWGG 2 cut(s) 489, 1053
Bst4CI ACNGT 4 cut(s) 301, 574, 1081, 1116
Bst6I CTCTTC 2 cut(s) 411, 510
BstACI GRCGYC 1 cut(s) 602
BstAPI GCANNNNNTGC 2 cut(s) 898, 990
BstBAI YACGTR 2 cut(s) 368, 1107
BstC8I GCNNGC 2 cut(s) 125, 894
BstDEI CTNAG 4 cut(s) 243, 329, 710, 969
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 3 cut(s) 150, 295, 1071
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 4 cut(s) 61, 295, 1033, 1043
BstMAI GTCTC 1 cut(s) 1070
BstMBI GATC 4 cut(s) 58, 292, 1030, 1040
BstMWI GCNNNNNNNGC 8 cut(s) 124, 343, 387, 884, 893, 898, 990, 999
BstNSI RCATGY 2 cut(s) 430, 896
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 609
BstV2I GAAGAC 1 cut(s) 605
BsuRI GGCC 3 cut(s) 228, 1002, 1052
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 89
BtsCI GGATG 3 cut(s) 150, 295, 1071
BtsI GCAGTG 3 cut(s) 125, 1021, 1105
BtsIMutI CAGTG 3 cut(s) 125, 1021, 1105
Cac8I GCNNGC 2 cut(s) 125, 894
CciI TCATGA 1 cut(s) 511
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 5 cut(s) 35, 226, 271, 574, 955
CseI GACGC 1 cut(s) 610
Csp6I GTAC 2 cut(s) 421, 1104
CviAII CATG 7 cut(s) 286, 427, 474, 490, 512, 578, 893
CviQI GTAC 2 cut(s) 421, 1104
DdeI CTNAG 4 cut(s) 243, 329, 710, 969
DpnI GATC 4 cut(s) 60, 294, 1032, 1042
DpnII GATC 4 cut(s) 58, 292, 1030, 1040
DraI TTTAAA 2 cut(s) 616, 1093
Eam1104I CTCTTC 2 cut(s) 411, 510
EarI CTCTTC 2 cut(s) 411, 510
Ecl136II GAGCTC 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 489, 1053
Eco147I AGGCCT 2 cut(s) 1002, 1052
Eco24I GRGCYC 2 cut(s) 413, 764
Eco47I GGWCC 4 cut(s) 35, 271, 574, 955
Eco53kI GAGCTC 1 cut(s) 762
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 762
EcoO109I RGGNCCY 2 cut(s) 226, 271
EcoT14I CCWWGG 2 cut(s) 489, 1053
EcoT38I GRGCYC 2 cut(s) 413, 764
ErhI CCWWGG 2 cut(s) 489, 1053
Esp3I CGTCTC 1 cut(s) 1070
FaeI CATG 7 cut(s) 289, 430, 477, 493, 515, 581, 896
FaqI GGGAC 1 cut(s) 284
FatI CATG 7 cut(s) 285, 426, 473, 489, 511, 577, 892
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 1 cut(s) 623
FokI GGATG 3 cut(s) 137, 302, 1078
FriOI GRGCYC 2 cut(s) 413, 764
Fsp4HI GCNGC 1 cut(s) 623
FspBI CTAG 2 cut(s) 215, 1038
GlaI GCGC 1 cut(s) 543
GluI GCNGC 1 cut(s) 623
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 3 cut(s) 228, 1002, 1052
HapII CCGG 1 cut(s) 497
HgaI GACGC 1 cut(s) 610
HhaI GCGC 1 cut(s) 544
Hin1I GRCGYC 1 cut(s) 602
Hin1II CATG 7 cut(s) 289, 430, 477, 493, 515, 581, 896
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HinfI GANTC 8 cut(s) 147, 450, 479, 557, 645, 832, 961, 1127
HpaII CCGG 1 cut(s) 497
HphI GGTGA 1 cut(s) 745
Hpy166II GTNNAC 1 cut(s) 1112
Hpy188I TCNGA 8 cut(s) 20, 185, 263, 332, 386, 447, 484, 827
Hpy188III TCNNGA 7 cut(s) 47, 311, 497, 512, 842, 907, 958
Hpy8I GTNNAC 1 cut(s) 1112
Hpy99I CGWCG 2 cut(s) 267, 305
HpyAV CCTTC 8 cut(s) 185, 217, 463, 518, 670, 877, 992, 1042
HpyCH4III ACNGT 4 cut(s) 301, 574, 1081, 1116
HpyCH4IV ACGT 3 cut(s) 265, 367, 1106
HpyCH4V TGCA 9 cut(s) 11, 118, 220, 285, 397, 810, 1027, 1098, 1124
HpyF10VI GCNNNNNNNGC 8 cut(s) 124, 343, 387, 884, 893, 898, 990, 999
HpyF3I CTNAG 4 cut(s) 243, 329, 710, 969
HpySE526I ACGT 3 cut(s) 265, 367, 1106
Hsp92I GRCGYC 1 cut(s) 602
Hsp92II CATG 7 cut(s) 289, 430, 477, 493, 515, 581, 896
HspAI GCGC 1 cut(s) 542
Kpn2I TCCGGA 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 4 cut(s) 58, 292, 1030, 1040
LmnI GCTCC 4 cut(s) 264, 343, 499, 502
LpnPI CCDG 8 cut(s) 51, 510, 618, 750, 920, 938, 971, 1013
Lsp1109I GCAGC 1 cut(s) 609
LweI GCATC 2 cut(s) 390, 819
MaeI CTAG 2 cut(s) 215, 1038
MaeII ACGT 3 cut(s) 265, 367, 1106
MaeIII GTNAC 2 cut(s) 64, 363
MalI GATC 4 cut(s) 60, 294, 1032, 1042
MboI GATC 4 cut(s) 58, 292, 1030, 1040
MboII GAAGA 8 cut(s) 94, 176, 428, 434, 527, 533, 607, 610
MhlI GDGCHC 3 cut(s) 125, 413, 764
MluCI AATT 7 cut(s) 178, 195, 617, 715, 935, 1005, 1088
MlyI GAGTC 3 cut(s) 488, 566, 639
MmeI TCCRAC 2 cut(s) 178, 286
MroI TCCGGA 1 cut(s) 496
MroXI GAANNNNTTC 2 cut(s) 25, 441
MseI TTAA 5 cut(s) 615, 938, 1083, 1092, 1151
MspI CCGG 1 cut(s) 497
Mva1269I GAATGC 1 cut(s) 995
MwoI GCNNNNNNNGC 8 cut(s) 124, 343, 387, 884, 893, 898, 990, 999
NcoI CCATGG 1 cut(s) 489
NdeII GATC 4 cut(s) 58, 292, 1030, 1040
NlaIII CATG 7 cut(s) 289, 430, 477, 493, 515, 581, 896
NlaIV GGNNCC 3 cut(s) 272, 495, 504
NspI RCATGY 2 cut(s) 430, 896
PaeI GCATGC 1 cut(s) 896
PagI TCATGA 1 cut(s) 511
PceI AGGCCT 2 cut(s) 1002, 1052
PciI ACATGT 1 cut(s) 426
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 995
PdmI GAANNNNTTC 2 cut(s) 25, 441
PfeI GAWTC 5 cut(s) 147, 450, 832, 961, 1127
PflMI CCANNNNNTGG 1 cut(s) 76
PkrI GCNGC 1 cut(s) 624
PleI GAGTC 3 cut(s) 487, 565, 639
PpsI GAGTC 3 cut(s) 487, 565, 639
Ppu21I YACGTR 2 cut(s) 368, 1107
PpuMI RGGWCCY 1 cut(s) 271
PscI ACATGT 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 271
PspN4I GGNNCC 3 cut(s) 272, 495, 504
PspPI GGNCC 5 cut(s) 35, 226, 271, 574, 955
PspPPI RGGWCCY 1 cut(s) 271
RsaI GTAC 2 cut(s) 422, 1105
RsaNI GTAC 2 cut(s) 421, 1104
SacI GAGCTC 1 cut(s) 764
SaqAI TTAA 5 cut(s) 615, 938, 1083, 1092, 1151
SatI GCNGC 1 cut(s) 623
Sau3AI GATC 4 cut(s) 58, 292, 1030, 1040
Sau96I GGNCC 5 cut(s) 35, 226, 271, 574, 955
SchI GAGTC 3 cut(s) 488, 566, 639
SduI GDGCHC 3 cut(s) 125, 413, 764
SfaNI GCATC 2 cut(s) 390, 819
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 4 cut(s) 35, 271, 574, 955
SmiI ATTTAAAT 1 cut(s) 1093
SpeI ACTAGT 1 cut(s) 214
SphI GCATGC 1 cut(s) 896
Sse9I AATT 7 cut(s) 178, 195, 617, 715, 935, 1005, 1088
SseBI AGGCCT 2 cut(s) 1002, 1052
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 914
SspMI CTAG 2 cut(s) 215, 1038
SstI GAGCTC 1 cut(s) 764
StuI AGGCCT 2 cut(s) 1002, 1052
StyI CCWWGG 2 cut(s) 489, 1053
SwaI ATTTAAAT 1 cut(s) 1093
TaaI ACNGT 4 cut(s) 301, 574, 1081, 1116
TaiI ACGT 3 cut(s) 268, 370, 1109
TaqI TCGA 4 cut(s) 303, 312, 404, 1043
TasI AATT 7 cut(s) 178, 195, 617, 715, 935, 1005, 1088
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 5 cut(s) 147, 450, 832, 961, 1127
Tru1I TTAA 5 cut(s) 615, 938, 1083, 1092, 1151
Tru9I TTAA 5 cut(s) 615, 938, 1083, 1092, 1151
TscAI CASTG 3 cut(s) 125, 1021, 1105
TseI GCWGC 1 cut(s) 622
TspDTI ATGAA 6 cut(s) 204, 450, 528, 594, 948, 1101
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 3 cut(s) 125, 1021, 1105
Van91I CCANNNNNTGG 1 cut(s) 76
VpaK11BI GGWCC 4 cut(s) 35, 271, 574, 955
XapI RAATTY 3 cut(s) 178, 715, 1088
XceI RCATGY 2 cut(s) 430, 896
XmnI GAANNNNTTC 2 cut(s) 25, 441
XspI CTAG 2 cut(s) 215, 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.