RLG00000011723
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018382
Physical Location & Seq
Forward (+)
12204726 .. 12205799
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011723

Sequence Viewer

Length: 927 bp
ATGGAGAAGAATAGCAAGTTCAAAACTCTCTTGGGCTACTTGCAGTGCTCGCTCAACTCCGTGAAAGAACGAATCATCCAACCCATAGAAGAAAAGCCCGAGAATTTCCCAGATGAACGAATTGAAAGTTTGATGATACTATTACGTGATGTGAATGCCATCAGAGGCTATATGCAATACTCGATGAGCCCCGAAGTGTACAACATGTTGAATGAAAATCTTCAGAGAATCATAGATACTTTTGAGAAGGCATGGGAGTTGGATACCATGGCTCCGGAGGAGCCAAATCATGAAGAGGAAGAAGGAATGGAACAAGCGGCGCTGTTGGTGTTTGAGACATTGTTTGAAACGGTCCATGAAGTGTTGAACAAGAAGAAGACGCCTGTGATGTTTAAATTGCTGCTTGGCGATATTTTTGACAGGCTCTTATTGTTGAAACCCTTTATGGAACAGAAGGCAAAAGCATTTGATGGCACAGACGAGGAACTAAGAAATTTCAAAGCAAAGATGGTGAAAGGGGTGGAGATTGTTCGCAAGAGCTCTGGGTTTGGTATGTGGGGTGGCTACAATAAGAGAAAGTATGAGTGCATCAACAACCTACTGGGACTGAATCAATCTCTTGAAAGACTATCATGTATAACGAGATGGGTGGAGGCAAGGAAGGCGATGCATTTCTTGCTTTTCCTGAAAAAATTGAAAACGGCTTCCAATGAATTAAAAAGCAGTTATCCGGTGCAGAATCAAACTGAGAGAGCTGATGGCAAAGAATGCGAGGAAGGCCTAATTGAAAGCAGTGTTGTGGTGCAGGATCAAGCTAAGAGGAAGGCCTTGGCTTTTAGGATGATGGTGGAGACGGCTAAATGGGCTAATGAATCTGAATGCAGTGGTGTGGTGCACAATCAAACTCGGTTTGCTGATTTAATGTGA

Protein Analysis

309

Amino Acids

35.73

Weight (kDa)

5.82

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018382)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g27330
rosa_laevigata RLG00000011723
rosa_multiflora Rmu_sc0000486.1_g000007
rosa_roxburghii Rroxscaffold_7G00178260
rosa_rugosa Rorug06G0258800
rosa_samantha Rh6AG371100 Rh6BG379000 Rh6CG384300 Rh6DG371600 Rh7CG403600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 1 cut(s) 317
AclWI GGATC 1 cut(s) 816
AcsI RAATTY 2 cut(s) 103, 493
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 1 cut(s) 200
AflIII ACRYGT 1 cut(s) 204
AloI GAACNNNNNNTCC 2 cut(s) 60, 92
AluBI AGCT 3 cut(s) 540, 755, 815
AluI AGCT 3 cut(s) 540, 755, 815
Alw21I GWGCWC 3 cut(s) 50, 542, 897
Alw26I GTCTC 2 cut(s) 329, 845
Alw44I GTGCAC 1 cut(s) 893
AlwI GGATC 1 cut(s) 816
Ama87I CYCGRG 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 274
AoxI GGCC 2 cut(s) 778, 825
ApaLI GTGCAC 1 cut(s) 893
ApeKI GCWGC 1 cut(s) 400
ApoI RAATTY 2 cut(s) 103, 493
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 897
BanII GRGCYC 2 cut(s) 191, 542
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 3 cut(s) 50, 542, 897
BbvI GCAGC 1 cut(s) 387
BccI CCATC 6 cut(s) 167, 464, 502, 639, 752, 838
BceAI ACGGC 2 cut(s) 717, 870
BciVI GTATCC 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 329, 845
BfoI RGCGCY 1 cut(s) 323
BfuI GTATCC 1 cut(s) 256
BisI GCNGC 2 cut(s) 318, 401
BlsI GCNGC 2 cut(s) 319, 402
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 273, 282
BmrI ACTGGG 1 cut(s) 611
BmsI GCATC 2 cut(s) 597, 657
BmuI ACTGGG 1 cut(s) 611
BpiI GAAGAC 1 cut(s) 383
BsaAI YACGTR 1 cut(s) 146
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 267, 828
BsaWI WCCGGW 2 cut(s) 274, 730
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bse1I ACTGG 1 cut(s) 606
BseAI TCCGGA 1 cut(s) 274
BseDI CCNNGG 2 cut(s) 267, 828
BseGI GGATG 2 cut(s) 75, 846
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 1 cut(s) 606
BseRI GAGGAG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 897
BseXI GCAGC 1 cut(s) 387
BsgI GTGCAG 2 cut(s) 755, 824
BshFI GGCC 2 cut(s) 780, 827
BsiHKAI GWGCWC 3 cut(s) 50, 542, 897
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 2 cut(s) 275, 731
BslFI GGGAC 1 cut(s) 618
BsmAI GTCTC 2 cut(s) 329, 845
BsmBI CGTCTC 1 cut(s) 845
BsmFI GGGAC 1 cut(s) 618
BsmI GAATGC 3 cut(s) 160, 773, 884
BsnI GGCC 2 cut(s) 780, 827
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 4 cut(s) 50, 191, 542, 897
Bsp13I TCCGGA 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 1 cut(s) 808
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 1 cut(s) 317
BspANI GGCC 2 cut(s) 780, 827
BspCNI CTCAG 1 cut(s) 739
BspEI TCCGGA 1 cut(s) 274
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 273, 282
BspPI GGATC 1 cut(s) 816
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 1 cut(s) 606
BssECI CCNNGG 2 cut(s) 267, 828
BssMI GATC 1 cut(s) 808
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 2 cut(s) 267, 828
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 1 cut(s) 288
BstACI GRCGYC 1 cut(s) 380
BstAPI GCANNNNNTGC 2 cut(s) 676, 768
BstAUI TGTACA 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 50
BstDEI CTNAG 3 cut(s) 488, 747, 816
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 2 cut(s) 75, 846
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 1 cut(s) 811
BstMAI GTCTC 2 cut(s) 329, 845
BstMBI GATC 1 cut(s) 808
BstMWI GCNNNNNNNGC 6 cut(s) 49, 662, 676, 768, 777, 863
BstNSI RCATGY 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 897
BstV1I GCAGC 1 cut(s) 387
BstV2I GAAGAC 1 cut(s) 383
BsuI GTATCC 1 cut(s) 256
BsuRI GGCC 2 cut(s) 780, 827
BtgI CCRYGG 1 cut(s) 267
BtgZI GCGATG 1 cut(s) 680
BtsCI GGATG 2 cut(s) 75, 846
BtsI GCAGTG 3 cut(s) 50, 799, 889
BtsIMutI CAGTG 3 cut(s) 50, 799, 889
Cac8I GCNNGC 1 cut(s) 50
CciI TCATGA 1 cut(s) 289
CfoI GCGC 1 cut(s) 322
Cfr13I GGNCC 1 cut(s) 352
CseI GACGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 199
CviAII CATG 6 cut(s) 205, 252, 268, 290, 356, 633
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 3 cut(s) 488, 747, 816
DpnI GATC 1 cut(s) 810
DpnII GATC 1 cut(s) 808
DraI TTTAAA 1 cut(s) 394
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Ecl136II GAGCTC 1 cut(s) 540
Eco130I CCWWGG 2 cut(s) 267, 828
Eco147I AGGCCT 2 cut(s) 780, 827
Eco24I GRGCYC 2 cut(s) 191, 542
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 540
Eco57I CTGAAG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 540
EcoT14I CCWWGG 2 cut(s) 267, 828
EcoT22I ATGCAT 1 cut(s) 672
EcoT38I GRGCYC 2 cut(s) 191, 542
ErhI CCWWGG 2 cut(s) 267, 828
Esp3I CGTCTC 1 cut(s) 845
FaeI CATG 6 cut(s) 208, 255, 271, 293, 359, 636
FaqI GGGAC 1 cut(s) 618
FatI CATG 6 cut(s) 204, 251, 267, 289, 355, 632
Fnu4HI GCNGC 2 cut(s) 318, 401
FokI GGATG 2 cut(s) 62, 853
FriOI GRGCYC 2 cut(s) 191, 542
Fsp4HI GCNGC 2 cut(s) 318, 401
GlaI GCGC 1 cut(s) 321
GluI GCNGC 2 cut(s) 318, 401
HaeII RGCGCY 1 cut(s) 323
HaeIII GGCC 2 cut(s) 780, 827
HapII CCGG 2 cut(s) 275, 731
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 6 cut(s) 208, 255, 271, 293, 359, 636
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HinfI GANTC 5 cut(s) 72, 228, 610, 739, 872
HpaII CCGG 2 cut(s) 275, 731
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 2 cut(s) 199, 895
Hpy188I TCNGA 3 cut(s) 164, 225, 877
Hpy188III TCNNGA 4 cut(s) 275, 290, 620, 685
Hpy8I GTNNAC 2 cut(s) 199, 895
HpyAV CCTTC 6 cut(s) 241, 296, 448, 655, 770, 817
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 8 cut(s) 43, 175, 588, 670, 736, 805, 882, 895
HpyF10VI GCNNNNNNNGC 6 cut(s) 49, 662, 676, 768, 777, 863
HpyF3I CTNAG 3 cut(s) 488, 747, 816
HpySE526I ACGT 1 cut(s) 145
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 6 cut(s) 208, 255, 271, 293, 359, 636
HspAI GCGC 1 cut(s) 320
Kpn2I TCCGGA 1 cut(s) 274
Kzo9I GATC 1 cut(s) 808
LmnI GCTCC 2 cut(s) 277, 280
LpnPI CCDG 9 cut(s) 123, 288, 396, 406, 528, 587, 698, 744, 791
Lsp1109I GCAGC 1 cut(s) 387
LweI GCATC 2 cut(s) 597, 657
MaeII ACGT 1 cut(s) 145
MalI GATC 1 cut(s) 810
MboI GATC 1 cut(s) 808
MboII GAAGA 7 cut(s) 19, 101, 212, 305, 311, 385, 388
MhlI GDGCHC 4 cut(s) 50, 191, 542, 897
MluCI AATT 7 cut(s) 103, 120, 395, 493, 692, 713, 783
MmeI TCCRAC 2 cut(s) 103, 240
MnlI CCTC 7 cut(s) 158, 271, 289, 475, 646, 766, 813
Mph1103I ATGCAT 1 cut(s) 672
MroI TCCGGA 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 3 cut(s) 393, 716, 920
MspI CCGG 2 cut(s) 275, 731
Mva1269I GAATGC 3 cut(s) 160, 773, 884
MwoI GCNNNNNNNGC 6 cut(s) 49, 662, 676, 768, 777, 863
NcoI CCATGG 1 cut(s) 267
NdeII GATC 1 cut(s) 808
NlaIII CATG 6 cut(s) 208, 255, 271, 293, 359, 636
NlaIV GGNNCC 2 cut(s) 273, 282
NsiI ATGCAT 1 cut(s) 672
NspI RCATGY 1 cut(s) 208
PagI TCATGA 1 cut(s) 289
PceI AGGCCT 2 cut(s) 780, 827
PciI ACATGT 1 cut(s) 204
PctI GAATGC 3 cut(s) 160, 773, 884
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 5 cut(s) 72, 228, 610, 739, 872
PkrI GCNGC 2 cut(s) 319, 402
Ppu21I YACGTR 1 cut(s) 146
PscI ACATGT 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 542
PspN4I GGNNCC 2 cut(s) 273, 282
PspPI GGNCC 1 cut(s) 352
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
SacI GAGCTC 1 cut(s) 542
SaqAI TTAA 3 cut(s) 393, 716, 920
SatI GCNGC 2 cut(s) 318, 401
Sau3AI GATC 1 cut(s) 808
Sau96I GGNCC 1 cut(s) 352
SduI GDGCHC 4 cut(s) 50, 191, 542, 897
SetI ASST 5 cut(s) 148, 542, 600, 757, 817
SfaNI GCATC 2 cut(s) 597, 657
SinI GGWCC 1 cut(s) 352
Sse9I AATT 7 cut(s) 103, 120, 395, 493, 692, 713, 783
SseBI AGGCCT 2 cut(s) 780, 827
SsiI CCGC 1 cut(s) 317
SstI GAGCTC 1 cut(s) 542
StuI AGGCCT 2 cut(s) 780, 827
StyI CCWWGG 2 cut(s) 267, 828
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 1 cut(s) 148
TaqI TCGA 1 cut(s) 182
TasI AATT 7 cut(s) 103, 120, 395, 493, 692, 713, 783
TatI WGTACW 1 cut(s) 198
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 5 cut(s) 72, 228, 610, 739, 872
Tru1I TTAA 3 cut(s) 393, 716, 920
Tru9I TTAA 3 cut(s) 393, 716, 920
TscAI CASTG 3 cut(s) 50, 799, 889
TseI GCWGC 1 cut(s) 400
TspDTI ATGAA 6 cut(s) 129, 228, 306, 372, 726, 885
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 3 cut(s) 50, 799, 889
VneI GTGCAC 1 cut(s) 893
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 2 cut(s) 103, 493
XceI RCATGY 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 219
Zsp2I ATGCAT 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.