Rh6AG371100

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0018382
Physical Location & Seq
Reverse (-)
56939823 .. 56940968
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG371100.1

Sequence Viewer

Length: 1146 bp
ATGGCGGTTGCAGCAATCGGAACGGCTTCGCTTGGACCAATCTTTCAAGAACTCTGCGATCAAGTTACCCAATCGATGGAGAAGAATCGCAAGTTCAAAACTCTCTTGGGCTACTTGCAGTGCTCGCTCAACTCCGTGAAAGAACGAATCATCCAACGTATAGAAGAAAAGCCCGAGAATTTCCCAGATGAACGAATTGAAAGTTTGATGATACTAGTGCAGAAGGGCCTAAAGCTAATAACTGAGAAGTTCTCCAAGCTCCGACGTTGGGACCTCTATTTCTGCATGGATGATCACACCGTCGAACTTCTCGGTTTGGATAGTGTTCTCAGAGAGCTGATGGAGCAGTTTTGGGTAATGGAGTTACGTGATGTGAATGACATCAGAGGCTATATGCAATACTCGATGAGCCCCGAAGTGTACAACATGTTGAATGAAAATCTTCAGAGAATCGTAGATACTTTTGAGAAGGCATGGGAGTCGGATACCATGGCTCCGGAGGAGCCAAATCATGAAGAGGAAGAAGGAATGGAACAAGCGACGCTGTTGGTGTTTGAGACATTGTTTGAAACGGTCCATGAAGTGTTGAACAAGAAGAAGACGCCTGTGATGTTTGAATTGCTGCTTGGCGATATTTTTGACAGACTCTTATTGTTGAAACCCTTTATGGAAAAGAAGGCAAAAGCATTTGATGGCACAGACGAGGAACTAAGAAATTTCAAAGCGAAGATGGTGAAAGGGGTGGAGATTGTTCGCAAGAGCTCTGGGTTTGGTATGTGGGGTGGCTACAATAAGAGAAAGTATGAGTGCATCAACAACCTACTCGGACTGAATCAATCTCTTGAAAGACTATTATGTATAATGAGATGGGTGGAGGCAAGGAAGGCGAGGCATTACTTGCTTTTCCTGAAATATTTGAAAACGGCTTCCAATGAATTAAAAAGCAGTTATCTGGTGCAGAATCAAACTGAGAGAGCTGATGGCAAAGAATGTGAAGAAGGCCTAATTGAAAGCAGTGTTGCGGTGCAGGATCAAGCTAAGAGGAAGGCCTTGGCTTTTCGGATGATGGTGGAGACGGTTAATGAAATTAAATGCAGTGGTACGTGTGGTGCAGAATCAAACTCTCGGTTTGCTGATTTAATGTGA

Protein Analysis

381

Amino Acids

44.26

Weight (kDa)

5.43

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 93 1.6e-07 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018382)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g27330
rosa_laevigata RLG00000011723
rosa_multiflora Rmu_sc0000486.1_g000007
rosa_roxburghii Rroxscaffold_7G00178260
rosa_rugosa Rorug06G0258800
rosa_samantha Rh6AG371100 Rh6BG379000 Rh6CG384300 Rh6DG371600 Rh7CG403600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 76
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 2 cut(s) 5, 1022
AclWI GGATC 1 cut(s) 1038
AcsI RAATTY 2 cut(s) 178, 715
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 602
AfaI GTAC 2 cut(s) 422, 1102
AfiI CCNNNNNNNGG 2 cut(s) 76, 268
AflIII ACRYGT 2 cut(s) 426, 1103
AhlI ACTAGT 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 609, 641
AloI GAACNNNNNNTCC 2 cut(s) 135, 167
AluBI AGCT 6 cut(s) 235, 259, 337, 762, 977, 1037
AluI AGCT 6 cut(s) 235, 259, 337, 762, 977, 1037
Alw21I GWGCWC 2 cut(s) 125, 764
Alw26I GTCTC 2 cut(s) 551, 1067
AlwI GGATC 1 cut(s) 1038
Ama87I CYCGRG 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 496
AoxI GGCC 3 cut(s) 226, 1000, 1047
ApeKI GCWGC 2 cut(s) 11, 622
ApoI RAATTY 2 cut(s) 178, 715
Asp700I GAANNNNTTC 2 cut(s) 25, 441
AspS9I GGNCC 4 cut(s) 35, 226, 271, 574
AsuHPI GGTGA 1 cut(s) 745
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 3 cut(s) 35, 271, 574
BanII GRGCYC 2 cut(s) 413, 764
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 2 cut(s) 125, 764
BbvI GCAGC 2 cut(s) 23, 609
BccI CCATC 7 cut(s) 70, 334, 686, 724, 861, 974, 1060
BceAI ACGGC 2 cut(s) 39, 939
BcgI CGANNNNNNTGC 2 cut(s) 462, 496
BciVI GTATCC 1 cut(s) 478
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 551, 1067
BcuI ACTAGT 1 cut(s) 214
BfaI CTAG 1 cut(s) 215
BfuI GTATCC 1 cut(s) 478
BisI GCNGC 2 cut(s) 12, 623
BlsI GCNGC 2 cut(s) 13, 624
Bme18I GGWCC 3 cut(s) 35, 271, 574
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 4 cut(s) 35, 226, 271, 574
BmiI GGNNCC 3 cut(s) 272, 495, 504
BmsI GCATC 1 cut(s) 819
BpiI GAAGAC 1 cut(s) 605
BplI GAGNNNNNCTC 2 cut(s) 236, 268
Bsa29I ATCGAT 1 cut(s) 74
BsaAI YACGTR 2 cut(s) 368, 1104
BsaHI GRCGYC 1 cut(s) 602
BsaJI CCNNGG 2 cut(s) 489, 1050
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 2 cut(s) 478, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 76, 268
BseAI TCCGGA 1 cut(s) 496
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 2 cut(s) 489, 1050
BseGI GGATG 3 cut(s) 150, 295, 1068
BseLI CCNNNNNNNGG 2 cut(s) 76, 268
BseMII CTCAG 3 cut(s) 234, 343, 960
BseRI GAGGAG 1 cut(s) 515
BseXI GCAGC 2 cut(s) 23, 609
BsgI GTGCAG 4 cut(s) 239, 977, 1046, 1131
BshFI GGCC 3 cut(s) 228, 1002, 1049
BshVI ATCGAT 1 cut(s) 74
BsiHKAI GWGCWC 2 cut(s) 125, 764
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 1 cut(s) 497
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 2 cut(s) 76, 268
BsmAI GTCTC 2 cut(s) 551, 1067
BsmBI CGTCTC 1 cut(s) 1067
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 3 cut(s) 228, 1002, 1049
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 3 cut(s) 125, 413, 764
Bsp13I TCCGGA 1 cut(s) 496
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 3 cut(s) 58, 292, 1030
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 2 cut(s) 5, 1022
BspANI GGCC 3 cut(s) 228, 1002, 1049
BspCNI CTCAG 3 cut(s) 235, 342, 961
BspDI ATCGAT 1 cut(s) 74
BspEI TCCGGA 1 cut(s) 496
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 272, 495, 504
BspPI GGATC 1 cut(s) 1038
BsrGI TGTACA 1 cut(s) 420
BssECI CCNNGG 2 cut(s) 489, 1050
BssMI GATC 3 cut(s) 58, 292, 1030
BssNI GRCGYC 1 cut(s) 602
BssT1I CCWWGG 2 cut(s) 489, 1050
Bst4CI ACNGT 3 cut(s) 301, 574, 1078
Bst6I CTCTTC 1 cut(s) 510
BstACI GRCGYC 1 cut(s) 602
BstAPI GCANNNNNTGC 1 cut(s) 898
BstAUI TGTACA 1 cut(s) 420
BstBAI YACGTR 2 cut(s) 368, 1104
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 5 cut(s) 243, 329, 710, 969, 1038
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 3 cut(s) 150, 295, 1068
BstKTI GATC 3 cut(s) 61, 295, 1033
BstMAI GTCTC 2 cut(s) 551, 1067
BstMBI GATC 3 cut(s) 58, 292, 1030
BstMWI GCNNNNNNNGC 5 cut(s) 11, 124, 343, 884, 898
BstNSI RCATGY 1 cut(s) 430
BstV1I GCAGC 2 cut(s) 23, 609
BstV2I GAAGAC 1 cut(s) 605
Bsu15I ATCGAT 1 cut(s) 74
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 3 cut(s) 228, 1002, 1049
BsuTUI ATCGAT 1 cut(s) 74
BtgI CCRYGG 1 cut(s) 489
BtsCI GGATG 3 cut(s) 150, 295, 1068
BtsI GCAGTG 3 cut(s) 125, 1021, 1102
BtsIMutI CAGTG 3 cut(s) 125, 1021, 1102
Cac8I GCNNGC 1 cut(s) 125
CciI TCATGA 1 cut(s) 511
Cfr13I GGNCC 4 cut(s) 35, 226, 271, 574
ClaI ATCGAT 1 cut(s) 74
CseI GACGC 2 cut(s) 550, 610
Csp6I GTAC 2 cut(s) 421, 1101
CviAII CATG 6 cut(s) 286, 427, 474, 490, 512, 578
CviQI GTAC 2 cut(s) 421, 1101
DdeI CTNAG 5 cut(s) 243, 329, 710, 969, 1038
DpnI GATC 3 cut(s) 60, 294, 1032
DpnII GATC 3 cut(s) 58, 292, 1030
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 762
Eco130I CCWWGG 2 cut(s) 489, 1050
Eco147I AGGCCT 2 cut(s) 1002, 1049
Eco24I GRGCYC 2 cut(s) 413, 764
Eco47I GGWCC 3 cut(s) 35, 271, 574
Eco53kI GAGCTC 1 cut(s) 762
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 762
EcoO109I RGGNCCY 2 cut(s) 226, 271
EcoT14I CCWWGG 2 cut(s) 489, 1050
EcoT38I GRGCYC 2 cut(s) 413, 764
ErhI CCWWGG 2 cut(s) 489, 1050
Esp3I CGTCTC 1 cut(s) 1067
FaeI CATG 6 cut(s) 289, 430, 477, 493, 515, 581
FaqI GGGAC 1 cut(s) 284
FatI CATG 6 cut(s) 285, 426, 473, 489, 511, 577
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 2 cut(s) 12, 623
FokI GGATG 3 cut(s) 137, 302, 1075
FriOI GRGCYC 2 cut(s) 413, 764
Fsp4HI GCNGC 2 cut(s) 12, 623
FspBI CTAG 1 cut(s) 215
GluI GCNGC 2 cut(s) 12, 623
HaeIII GGCC 3 cut(s) 228, 1002, 1049
HapII CCGG 1 cut(s) 497
HgaI GACGC 2 cut(s) 550, 610
Hin1I GRCGYC 1 cut(s) 602
Hin1II CATG 6 cut(s) 289, 430, 477, 493, 515, 581
HinfI GANTC 8 cut(s) 85, 147, 450, 479, 645, 832, 961, 1115
HpaII CCGG 1 cut(s) 497
HphI GGTGA 1 cut(s) 745
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 8 cut(s) 20, 263, 332, 386, 447, 484, 827, 1062
Hpy188III TCNNGA 5 cut(s) 47, 497, 512, 842, 907
Hpy8I GTNNAC 1 cut(s) 421
Hpy99I CGWCG 3 cut(s) 267, 305, 544
HpyAV CCTTC 7 cut(s) 217, 463, 518, 670, 877, 992, 1039
HpyCH4III ACNGT 3 cut(s) 301, 574, 1078
HpyCH4IV ACGT 4 cut(s) 157, 265, 367, 1103
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 124, 343, 884, 898
HpyF3I CTNAG 5 cut(s) 243, 329, 710, 969, 1038
HpySE526I ACGT 4 cut(s) 157, 265, 367, 1103
Hsp92I GRCGYC 1 cut(s) 602
Hsp92II CATG 6 cut(s) 289, 430, 477, 493, 515, 581
Kpn2I TCCGGA 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 3 cut(s) 58, 292, 1030
LmnI GCTCC 4 cut(s) 264, 343, 499, 502
LpnPI CCDG 7 cut(s) 198, 510, 618, 750, 920, 938, 1013
Lsp1109I GCAGC 2 cut(s) 23, 609
LweI GCATC 1 cut(s) 819
MaeI CTAG 1 cut(s) 215
MaeII ACGT 4 cut(s) 157, 265, 367, 1103
MaeIII GTNAC 2 cut(s) 64, 363
MalI GATC 3 cut(s) 60, 294, 1032
MboI GATC 3 cut(s) 58, 292, 1030
MboII GAAGA 9 cut(s) 94, 176, 434, 527, 533, 607, 610, 739, 1007
MhlI GDGCHC 3 cut(s) 125, 413, 764
MluCI AATT 7 cut(s) 178, 195, 617, 715, 935, 1005, 1086
MlyI GAGTC 2 cut(s) 488, 639
MmeI TCCRAC 3 cut(s) 178, 286, 462
MnlI CCTC 8 cut(s) 284, 380, 493, 511, 697, 868, 882, 1035
MroI TCCGGA 1 cut(s) 496
MroXI GAANNNNTTC 2 cut(s) 25, 441
MseI TTAA 4 cut(s) 938, 1080, 1089, 1139
MspI CCGG 1 cut(s) 497
MwoI GCNNNNNNNGC 5 cut(s) 11, 124, 343, 884, 898
NcoI CCATGG 1 cut(s) 489
NdeII GATC 3 cut(s) 58, 292, 1030
NlaIII CATG 6 cut(s) 289, 430, 477, 493, 515, 581
NlaIV GGNNCC 3 cut(s) 272, 495, 504
NspI RCATGY 1 cut(s) 430
PagI TCATGA 1 cut(s) 511
PceI AGGCCT 2 cut(s) 1002, 1049
PciI ACATGT 1 cut(s) 426
PdmI GAANNNNTTC 2 cut(s) 25, 441
PfeI GAWTC 6 cut(s) 85, 147, 450, 832, 961, 1115
PflMI CCANNNNNTGG 1 cut(s) 76
PkrI GCNGC 2 cut(s) 13, 624
PleI GAGTC 2 cut(s) 487, 639
PpsI GAGTC 2 cut(s) 487, 639
Ppu21I YACGTR 2 cut(s) 368, 1104
PpuMI RGGWCCY 1 cut(s) 271
PscI ACATGT 1 cut(s) 426
Psp124BI GAGCTC 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 271
PspN4I GGNNCC 3 cut(s) 272, 495, 504
PspPI GGNCC 4 cut(s) 35, 226, 271, 574
PspPPI RGGWCCY 1 cut(s) 271
RsaI GTAC 2 cut(s) 422, 1102
RsaNI GTAC 2 cut(s) 421, 1101
SacI GAGCTC 1 cut(s) 764
SaqAI TTAA 4 cut(s) 938, 1080, 1089, 1139
SatI GCNGC 2 cut(s) 12, 623
Sau3AI GATC 3 cut(s) 58, 292, 1030
Sau96I GGNCC 4 cut(s) 35, 226, 271, 574
SchI GAGTC 2 cut(s) 488, 639
SduI GDGCHC 3 cut(s) 125, 413, 764
SfaNI GCATC 1 cut(s) 819
SinI GGWCC 3 cut(s) 35, 271, 574
SpeI ACTAGT 1 cut(s) 214
Sse9I AATT 7 cut(s) 178, 195, 617, 715, 935, 1005, 1086
SseBI AGGCCT 2 cut(s) 1002, 1049
SsiI CCGC 2 cut(s) 5, 1022
SspI AATATT 1 cut(s) 914
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 764
StuI AGGCCT 2 cut(s) 1002, 1049
StyI CCWWGG 2 cut(s) 489, 1050
TaaI ACNGT 3 cut(s) 301, 574, 1078
TaiI ACGT 4 cut(s) 160, 268, 370, 1106
TaqI TCGA 3 cut(s) 74, 303, 404
TasI AATT 7 cut(s) 178, 195, 617, 715, 935, 1005, 1086
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 6 cut(s) 85, 147, 450, 832, 961, 1115
Tru1I TTAA 4 cut(s) 938, 1080, 1089, 1139
Tru9I TTAA 4 cut(s) 938, 1080, 1089, 1139
TscAI CASTG 3 cut(s) 125, 1021, 1102
TseI GCWGC 2 cut(s) 11, 622
TspDTI ATGAA 6 cut(s) 204, 450, 528, 594, 948, 1098
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 3 cut(s) 125, 1021, 1102
Van91I CCANNNNNTGG 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 35, 271, 574
XapI RAATTY 2 cut(s) 178, 715
XceI RCATGY 1 cut(s) 430
XmnI GAANNNNTTC 2 cut(s) 25, 441
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.