FvH4_2g27620

WD40-like Beta Propeller Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016112
Physical Location & Seq
Reverse (-)
21870284 .. 21873380
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27620.t1

Sequence Viewer

Length: 2151 bp
ATGAACATTTATACACACAAAAACACTGCCACCCCCTCACTGAACTCCACCCACAGAATCAAACACAACATGAAACCCAATTCAATTCTTCATCTTTTGCTCCATTTGCTGCTGCTTAGCTTCATCACAGCTGAAGACCATGGCCCTTCCAGCATCGTCTTCACCACCAACGGCAGATTAGATTACTACTTCGACATCTTCACCCTCCCTGTTGCTCACCCAACTCACCCAATCGCCGAAACCCGAATCACAGACGGCCAGTCCGTCAACTTCAACGGCCACTTCCCCTCCTCCACTTTCTTTCCCAACCAAACCCGACTCCGATCCCCAAACCGACCCGACCCGTCTCTCCAAATCATCTACGTCACCGAGCGCAACGGCTTCTCCAACATCTTCTACGACGCCGTTTACTTCGACGATTCTCCCTCCAGAAAAAGGTCCGCGCTTGAAGTCGTGGACCGGGTTCAGCTCCCTCTGCTGGGATCGGACCGGGTTTCCATGAAAGACAAGCCCAGCTTGACCGGGGAGTATTTAGTATACGTGTCAACTCACGAAGACTCCGGCGTTCCCAGGACGAGCTCAGCTGCGGTGTACGCGACTGAGCTGAGCTCGGGTTTGACCCGGAGGCTGACTCCTCACGGCGTCGCGGACTTCAGCCCGGCGGTGTCGCCTTCCGGAGTTTGGACGGCGGCGGCGTCGTATGGGGAGAAAGGCTGGGACGGCGAGGTCGAGGAGCTCAGTACAGACATCTACGTGTTCTTGACTCGCGACGGGACTCGCCGAGTCAAGGTGGTCGAGCACGGCGGCTGGCCGAGCTGGGTTGACGAGTCAACGATTTACTTTCACAGAAGAGGAGAAGACCAGTGGTGGAGTATTTACCGGGCGATTTTACCGAAACAGGGGGCGGTTTCTACGGAATCAGTTGTGGTGGAGCGAATCACGCCGCCGGGTCTCCACGCGTTCACGCCCGCCACGTCACCCGGGAACCACGCCTTCATCGCCGTGGCCACCAGAAGGCCCTCCACGAGCTTCCGCCACGTGGAGCTTTTCGACTTGGTGAAGGGCGAGTTCAAGGAGCTGACGCGGCTCGTCTCGCCGAGCACACACCACTTCAACCCGTTCATCTCCCCCGATTCGGGTCGGGTCGGGTACCACAAGTGCAGAGGCAACTTAAACGGAAAGAAAAGCCCGAAGCTTTTTCTACAAAACATCCACACCGCGATTCCGAACCTCTCCGTCCTCAGAGTCGACGGTACTTTTCCTTCGTTCTCACCCTCGGGTGATCGTATCGCCTTCGCGAACTTACCGGGTGTCTACGTCGTGAACCGCGACGGTTCGAACCGGCGGGAGGTCTACCCTGGAAATGCGTTCTCGACGTCGTGGGACCCGGTTCGCAAGGGCGTAGTATACACCGGCGCCGGGCCGGAATTCGCACCGGAAAGCACTGAGGTTGATATCATCGCCATCGACGTTGATCAAATCGCCGTCAAGAAGTTGACCACAAACGGAAAGAACAACGCTTTCCCTTCACCCTCGCCGGACGGGAAACGGATCGTCTTCCGGTCGGGTCGGTCGGGTCATAAGAATCTCTACATAATGGACGCCGAGGAAGGTGAGAGAATCGGGCTGCAGAGGCTGACGGAGGGCCCATGGACGGACACGATGTGTAACTGGTCGCCGGACGGCGACTGGATAGCGTTCGCTTCCGACCGGGAAAACCCCGGAAGCGGGAGCTTCGAGTTGTACGTGATCCACCCGAACGGAACCGGGTTGAGAAAAGTGATCCAAAGCGGGTCGGGCGGTCGGACTAACCACCCGTGGTTCAGCCCGGATGGGAAGAGCTTGGTTTTCACTTCGGATTATGGGGCAATATCGGCTGAGCCTATCTCAAACCCGCATCATTATCAGCCGTACGGTGAGATTTTTACGGTGAAAGTTGACGGCTCTGATCTTAGGAGGTTGACGCAGAATTCGTTCGAGGATGGAACACCGGTGTGGGTTCCGAGCTTCATTAGGCCGTCGGATGTAGAGTGGCCGATTGAGAGGCCGAGCTGCGGCTTCGATGATTTGCATTGCCTCAGTAATTTTCCGAGCTATGGTGGTGTTGGGGCTGTGCCTTGGCTCTCAAGCAAGCCTCAGTGTGCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PD40

Protein Analysis

717

Amino Acids

79.28

Weight (kDa)

6.61

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tricorn_N PF26549 485 - 667 5.4e-21 Tricorn protease N-terminal beta propeller domain
Tricorn_2nd PF26550 486 - 620 1e-08 Tricorn protease second beta propeller domain
PD40 PF07676 497 - 532 7.5e-08 WD40-like Beta Propeller Repeat
PD40 PF07676 544 - 583 9.9e-08 WD40-like Beta Propeller Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016112)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21680
fragaria_vesca FvH4_2g27620
malus_domestica MD15G1344100.v1.1
prunus_persica Prupe.1G491500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0296441
rosa_laevigata RLG00000011693
rosa_multiflora Rmu_sc0028875.1_g000001
rosa_roxburghii Rroxscaffold_7G00171530 Rroxscaffold_7G00178600
rosa_rugosa Rorug06G0262400
rosa_samantha Rh6AG373600 Rh6BG382400 Rh6CG387200 Rh6DG374900
rosa_wichuraiana Rw6G032600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 725
AatII GACGTC 1 cut(s) 1379
Acc65I GGTACC 1 cut(s) 1149
AccB1I GGYRCC 2 cut(s) 1149, 1415
AccI GTMKAC 5 cut(s) 537, 1248, 1314, 1353, 1407
AccII CGCG 9 cut(s) 443, 596, 647, 768, 959, 1084, 1220, 1298, 1329
AccIII TCCGGA 1 cut(s) 674
AclWI GGATC 5 cut(s) 318, 490, 1559, 1744, 1777
AcoI YGGCCR 5 cut(s) 256, 277, 809, 1005, 2033
AcsI RAATTY 2 cut(s) 1427, 1969
AcuI CTGAAG 2 cut(s) 153, 637
AcvI CACGTG 1 cut(s) 1039
AcyI GRCGYC 6 cut(s) 402, 642, 695, 1376, 1416, 1602
AdeI CACNNNGTG 1 cut(s) 1665
AfaI GTAC 6 cut(s) 593, 742, 1151, 1255, 1745, 1913
AflIII ACRYGT 3 cut(s) 540, 753, 957
AgeI ACCGGT 1 cut(s) 1990
AgsI TTSAA 5 cut(s) 84, 274, 449, 1072, 1114
AhdI GACNNNNNGTC 1 cut(s) 259
AjiI CACGTC 1 cut(s) 975
AjnI CCWGG 2 cut(s) 569, 1357
AleI CACNNNNGTG 1 cut(s) 1993
Alw21I GWGCWC 5 cut(s) 581, 611, 738, 801, 1103
Alw26I GTCTC 3 cut(s) 351, 956, 1096
AlwI GGATC 5 cut(s) 318, 490, 1559, 1744, 1777
AlwNI CAGNNNCTG 1 cut(s) 1636
Ama87I CYCGRG 3 cut(s) 610, 980, 1276
Aor13HI TCCGGA 1 cut(s) 674
ApaI GGGCCC 1 cut(s) 1649
ApeKI GCWGC 6 cut(s) 109, 112, 584, 1627, 2052, 2144
ApoI RAATTY 2 cut(s) 1427, 1969
ArsI GACNNNNNNTTYG 2 cut(s) 1954, 1986
AsiGI ACCGGT 1 cut(s) 1990
Asp700I GAANNNNTTC 1 cut(s) 1973
Asp718I GGTACC 1 cut(s) 1149
AspLEI GCGC 3 cut(s) 375, 445, 1418
AspS9I GGNCC 9 cut(s) 143, 438, 457, 487, 1017, 1384, 1421, 1645, 1646
AsuII TTCGAA 1 cut(s) 1337
AvaI CYCGRG 3 cut(s) 610, 980, 1276
AvaII GGWCC 4 cut(s) 438, 457, 487, 1384
BaeGI GKGCMC 1 cut(s) 1649
BalI TGGCCA 1 cut(s) 1007
BanI GGYRCC 2 cut(s) 1149, 1415
BanII GRGCYC 4 cut(s) 581, 611, 738, 1649
BarI GAAGNNNNNNTAC 2 cut(s) 1246, 1278
BauI CACGAG 1 cut(s) 1024
BbrPI CACGTG 1 cut(s) 1039
BbsI GAAGAC 5 cut(s) 141, 151, 561, 864, 1549
Bbv12I GWGCWC 5 cut(s) 581, 611, 738, 801, 1103
BbvI GCAGC 6 cut(s) 96, 99, 571, 1614, 2039, 2131
BccI CCATC 3 cut(s) 1472, 1826, 1976
BciT130I CCWGG 2 cut(s) 571, 1359
BclI TGATCA 1 cut(s) 1474
BcoDI GTCTC 3 cut(s) 351, 956, 1096
BfaI CTAG 1 cut(s) 2149
BfmI CTRYAG 1 cut(s) 1628
BfoI RGCGCY 1 cut(s) 1419
BlpI GCTNAGC 4 cut(s) 116, 580, 605, 1878
Bme18I GGWCC 4 cut(s) 438, 457, 487, 1384
BmeRI GACNNNNNGTC 1 cut(s) 259
BmeT110I CYCGRG 3 cut(s) 610, 980, 1276
BmgBI CACGTC 1 cut(s) 975
BmgT120I GGNCC 9 cut(s) 143, 438, 457, 487, 1017, 1384, 1421, 1645, 1646
BmiI GGNNCC 8 cut(s) 986, 1151, 1385, 1386, 1417, 1647, 1765, 2001
BmsI GCATC 2 cut(s) 162, 1906
BpiI GAAGAC 5 cut(s) 141, 151, 561, 864, 1549
BplI GAGNNNNNCTC 6 cut(s) 593, 625, 616, 648, 1871, 1903
BpmI CTGGAG 1 cut(s) 412
Bpu1102I GCTNAGC 4 cut(s) 116, 580, 605, 1878
Bpu14I TTCGAA 1 cut(s) 1337
BpuEI CTTGAG 1 cut(s) 2110
BsaAI YACGTR 4 cut(s) 541, 754, 1039, 1747
BsaHI GRCGYC 6 cut(s) 402, 642, 695, 1376, 1416, 1602
BsaI GGTCTC 1 cut(s) 956
BsaWI WCCGGW 4 cut(s) 674, 1435, 1560, 1990
BsaXI ACNNNNNCTCC 6 cut(s) 272, 302, 368, 398, 542, 572
Bse118I RCCGGY 3 cut(s) 1341, 1412, 1990
Bse1I ACTGG 4 cut(s) 259, 862, 1676, 1694
Bse3DI GCAATG 1 cut(s) 2071
BseAI TCCGGA 1 cut(s) 674
BseBI CCWGG 2 cut(s) 571, 1359
BseGI GGATG 4 cut(s) 1209, 1837, 1987, 2029
BseMI GCAATG 1 cut(s) 2071
BseMII CTCAG 9 cut(s) 591, 594, 596, 751, 1255, 1437, 1869, 2092, 2150
BseNI ACTGG 4 cut(s) 259, 862, 1676, 1694
BseRI GAGGAG 4 cut(s) 280, 624, 746, 867
BseSI GKGCMC 1 cut(s) 1649
BseXI GCAGC 6 cut(s) 96, 99, 571, 1614, 2039, 2131
BseYI CCCAGC 4 cut(s) 478, 512, 714, 816
BsgI GTGCAG 1 cut(s) 1180
Bsh1236I CGCG 9 cut(s) 443, 596, 647, 768, 959, 1084, 1220, 1298, 1329
Bsh1285I CGRYCG 4 cut(s) 1565, 1574, 1711, 1804
BshNI GGYRCC 2 cut(s) 1149, 1415
BshTI ACCGGT 1 cut(s) 1990
BsiEI CGRYCG 4 cut(s) 1565, 1574, 1711, 1804
BsiHKAI GWGCWC 5 cut(s) 581, 611, 738, 801, 1103
BsiHKCI CYCGRG 3 cut(s) 610, 980, 1276
BsiWI CGTACG 1 cut(s) 1911
BslFI GGGAC 3 cut(s) 731, 787, 1397
BsmAI GTCTC 3 cut(s) 351, 956, 1096
BsmBI CGTCTC 2 cut(s) 351, 1096
BsmFI GGGAC 3 cut(s) 731, 787, 1397
Bso31I GGTCTC 1 cut(s) 956
BsoBI CYCGRG 3 cut(s) 610, 980, 1276
Bsp119I TTCGAA 1 cut(s) 1337
Bsp120I GGGCCC 1 cut(s) 1645
Bsp1286I GDGCHC 6 cut(s) 581, 611, 738, 801, 1103, 1649
Bsp13I TCCGGA 1 cut(s) 674
Bsp143I GATC 8 cut(s) 323, 482, 1282, 1474, 1551, 1749, 1782, 1948
Bsp1720I GCTNAGC 4 cut(s) 116, 580, 605, 1878
Bsp19I CCATGG 2 cut(s) 139, 1649
Bsp68I TCGCGA 2 cut(s) 768, 1298
BspCNI CTCAG 9 cut(s) 592, 593, 597, 750, 1254, 1438, 1870, 2091, 2149
BspEI TCCGGA 1 cut(s) 674
BspFNI CGCG 9 cut(s) 443, 596, 647, 768, 959, 1084, 1220, 1298, 1329
BspLI GGNNCC 8 cut(s) 986, 1151, 1385, 1386, 1417, 1647, 1765, 2001
BspMAI CTGCAG 1 cut(s) 1632
BspPI GGATC 5 cut(s) 318, 490, 1559, 1744, 1777
BspQI GCTCTTC 1 cut(s) 1832
BspT104I TTCGAA 1 cut(s) 1337
BspT107I GGYRCC 2 cut(s) 1149, 1415
BspTNI GGTCTC 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 2071
BsrFI RCCGGY 3 cut(s) 1341, 1412, 1990
BsrI ACTGG 4 cut(s) 259, 862, 1676, 1694
BssAI RCCGGY 3 cut(s) 1341, 1412, 1990
BssMI GATC 8 cut(s) 323, 482, 1282, 1474, 1551, 1749, 1782, 1948
BssNAI GTATAC 2 cut(s) 538, 1408
BssNI GRCGYC 6 cut(s) 402, 642, 695, 1376, 1416, 1602
BssSI CACGAG 1 cut(s) 1024
BssT1I CCWWGG 3 cut(s) 139, 1649, 2117
Bst1107I GTATAC 2 cut(s) 538, 1408
Bst2BI CACGAG 1 cut(s) 1024
Bst2UI CCWGG 2 cut(s) 571, 1359
Bst4CI ACNGT 4 cut(s) 1253, 1334, 1916, 1930
Bst6I CTCTTC 2 cut(s) 844, 1832
BstACI GRCGYC 6 cut(s) 402, 642, 695, 1376, 1416, 1602
BstBAI YACGTR 4 cut(s) 541, 754, 1039, 1747
BstBI TTCGAA 1 cut(s) 1337
BstC8I GCNNGC 3 cut(s) 809, 969, 2132
BstDSI CCRYGG 4 cut(s) 139, 1002, 1649, 1817
BstF5I GGATG 4 cut(s) 1209, 1837, 1987, 2029
BstFNI CGCG 9 cut(s) 443, 596, 647, 768, 959, 1084, 1220, 1298, 1329
BstH2I RGCGCY 1 cut(s) 1419
BstHHI GCGC 3 cut(s) 375, 445, 1418
BstKTI GATC 8 cut(s) 326, 485, 1285, 1477, 1554, 1752, 1785, 1951
BstMAI GTCTC 3 cut(s) 351, 956, 1096
BstMBI GATC 8 cut(s) 323, 482, 1282, 1474, 1551, 1749, 1782, 1948
BstMCI CGRYCG 4 cut(s) 1565, 1574, 1711, 1804
BstNI CCWGG 2 cut(s) 571, 1359
BstSFI CTRYAG 1 cut(s) 1628
BstSLI GKGCMC 1 cut(s) 1649
BstUI CGCG 9 cut(s) 443, 596, 647, 768, 959, 1084, 1220, 1298, 1329
BstV1I GCAGC 6 cut(s) 96, 99, 571, 1614, 2039, 2131
BstV2I GAAGAC 5 cut(s) 141, 151, 561, 864, 1549
BstZ17I GTATAC 2 cut(s) 538, 1408
BtgI CCRYGG 4 cut(s) 139, 1002, 1649, 1817
BtgZI GCGATG 2 cut(s) 982, 1444
BtrI CACGTC 1 cut(s) 975
BtsCI GGATG 4 cut(s) 1209, 1837, 1987, 2029
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 5 cut(s) 24, 38, 869, 1443, 2144
BtuMI TCGCGA 2 cut(s) 768, 1298
Cac8I GCNNGC 3 cut(s) 809, 969, 2132
CaiI CAGNNNCTG 1 cut(s) 1636
CfoI GCGC 3 cut(s) 375, 445, 1418
Cfr10I RCCGGY 3 cut(s) 1341, 1412, 1990
Cfr13I GGNCC 9 cut(s) 143, 438, 457, 487, 1017, 1384, 1421, 1645, 1646
Cfr9I CCCGGG 1 cut(s) 980
CpoI CGGWCCG 1 cut(s) 487
CseI GACGC 6 cut(s) 410, 631, 684, 1090, 1610, 1972
Csp6I GTAC 6 cut(s) 592, 741, 1150, 1254, 1744, 1912
CspAI ACCGGT 1 cut(s) 1990
CspI CGGWCCG 1 cut(s) 487
CviAII CATG 4 cut(s) 70, 140, 499, 1650
CviQI GTAC 6 cut(s) 592, 741, 1150, 1254, 1744, 1912
DinI GGCGCC 1 cut(s) 1417
DpnI GATC 8 cut(s) 325, 484, 1284, 1476, 1553, 1751, 1784, 1950
DpnII GATC 8 cut(s) 323, 482, 1282, 1474, 1551, 1749, 1782, 1948
DraIII CACNNNGTG 1 cut(s) 1665
DrdI GACNNNNNNGTC 1 cut(s) 725
DriI GACNNNNNGTC 1 cut(s) 259
DseDI GACNNNNNNGTC 1 cut(s) 725
EaeI YGGCCR 5 cut(s) 256, 277, 809, 1005, 2033
Eam1104I CTCTTC 2 cut(s) 844, 1832
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 2 cut(s) 844, 1832
EciI GGCGGA 1 cut(s) 1022
Ecl136II GAGCTC 3 cut(s) 579, 609, 736
Eco130I CCWWGG 3 cut(s) 139, 1649, 2117
Eco24I GRGCYC 4 cut(s) 581, 611, 738, 1649
Eco31I GGTCTC 1 cut(s) 956
Eco32I GATATC 1 cut(s) 1456
Eco47I GGWCC 4 cut(s) 438, 457, 487, 1384
Eco53kI GAGCTC 3 cut(s) 579, 609, 736
Eco57I CTGAAG 2 cut(s) 153, 637
Eco72I CACGTG 1 cut(s) 1039
Eco88I CYCGRG 3 cut(s) 610, 980, 1276
EcoICRI GAGCTC 3 cut(s) 579, 609, 736
EcoO109I RGGNCCY 3 cut(s) 1017, 1384, 1645
EcoRI GAATTC 2 cut(s) 1427, 1969
EcoRII CCWGG 2 cut(s) 569, 1357
EcoRV GATATC 1 cut(s) 1456
EcoT14I CCWWGG 3 cut(s) 139, 1649, 2117
EcoT38I GRGCYC 4 cut(s) 581, 611, 738, 1649
EgeI GGCGCC 1 cut(s) 1417
EheI GGCGCC 1 cut(s) 1417
ErhI CCWWGG 3 cut(s) 139, 1649, 2117
Esp3I CGTCTC 2 cut(s) 351, 1096
FaeI CATG 4 cut(s) 73, 143, 502, 1653
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 3 cut(s) 731, 787, 1397
FatI CATG 4 cut(s) 69, 139, 498, 1649
FauI CCCGC 5 cut(s) 976, 1338, 1721, 1784, 1902
FbaI TGATCA 1 cut(s) 1474
FblI GTMKAC 5 cut(s) 537, 1248, 1314, 1353, 1407
FokI GGATG 4 cut(s) 1196, 1844, 1994, 2036
FriOI GRGCYC 4 cut(s) 581, 611, 738, 1649
FspBI CTAG 1 cut(s) 2149
GlaI GCGC 3 cut(s) 374, 444, 1417
GsaI CCCAGC 4 cut(s) 482, 516, 718, 820
GsuI CTGGAG 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 1419
HgaI GACGC 6 cut(s) 410, 631, 684, 1090, 1610, 1972
HhaI GCGC 3 cut(s) 375, 445, 1418
Hin1I GRCGYC 6 cut(s) 402, 642, 695, 1376, 1416, 1602
Hin1II CATG 4 cut(s) 73, 143, 502, 1653
Hin6I GCGC 3 cut(s) 373, 443, 1416
HinP1I GCGC 3 cut(s) 373, 443, 1416
HincII GTYRAC 8 cut(s) 268, 546, 823, 831, 1249, 1497, 1939, 1962
HindII GTYRAC 8 cut(s) 268, 546, 823, 831, 1249, 1497, 1939, 1962
HindIII AAGCTT 1 cut(s) 1193
Hpy188III TCNNGA 9 cut(s) 429, 551, 675, 760, 767, 1297, 1321, 1372, 1489
HpyAV CCTTC 9 cut(s) 156, 681, 1003, 1008, 1054, 1272, 1303, 1536, 1604
HpyCH4III ACNGT 4 cut(s) 1253, 1334, 1916, 1930
HpyCH4IV ACGT 9 cut(s) 363, 540, 753, 974, 1038, 1317, 1376, 1470, 1746
HpyCH4V TGCA 3 cut(s) 1161, 1630, 2071
HpySE526I ACGT 9 cut(s) 363, 540, 753, 974, 1038, 1317, 1376, 1470, 1746
Hsp92I GRCGYC 6 cut(s) 402, 642, 695, 1376, 1416, 1602
Hsp92II CATG 4 cut(s) 73, 143, 502, 1653
HspAI GCGC 3 cut(s) 373, 443, 1416
KasI GGCGCC 1 cut(s) 1415
KflI GGGWCCC 1 cut(s) 1384
Kpn2I TCCGGA 1 cut(s) 674
KpnI GGTACC 1 cut(s) 1153
Ksp22I TGATCA 1 cut(s) 1474
Kzo9I GATC 8 cut(s) 323, 482, 1282, 1474, 1551, 1749, 1782, 1948
LguI GCTCTTC 1 cut(s) 1832
LmnI GCTCC 7 cut(s) 105, 474, 733, 931, 1042, 1075, 1731
Lsp1109I GCAGC 6 cut(s) 96, 99, 571, 1614, 2039, 2131
LweI GCATC 2 cut(s) 162, 1906
MaeI CTAG 1 cut(s) 2149
MaeII ACGT 9 cut(s) 363, 540, 753, 974, 1038, 1317, 1376, 1470, 1746
MaeIII GTNAC 3 cut(s) 364, 975, 1667
MalI GATC 8 cut(s) 325, 484, 1284, 1476, 1553, 1751, 1784, 1950
MboI GATC 8 cut(s) 323, 482, 1282, 1474, 1551, 1749, 1782, 1948
MhlI GDGCHC 6 cut(s) 581, 611, 738, 801, 1103, 1649
MlsI TGGCCA 1 cut(s) 1007
MluCI AATT 5 cut(s) 79, 84, 1427, 1969, 2083
MluI ACGCGT 1 cut(s) 957
MluNI TGGCCA 1 cut(s) 1007
Mly113I GGCGCC 1 cut(s) 1416
MlyI GAGTC 8 cut(s) 312, 551, 625, 757, 769, 792, 836, 1254
MmeI TCCRAC 4 cut(s) 411, 1731, 1784, 2001
Mox20I TGGCCA 1 cut(s) 1007
MroI TCCGGA 1 cut(s) 674
MroXI GAANNNNTTC 1 cut(s) 1973
MscI TGGCCA 1 cut(s) 1007
MseI TTAA 1 cut(s) 1172
MslI CAYNNNNRTG 3 cut(s) 752, 1001, 1993
Msp20I TGGCCA 1 cut(s) 1007
MspA1I CMGCKG 2 cut(s) 131, 584
MvaI CCWGG 2 cut(s) 571, 1359
MvnI CGCG 9 cut(s) 443, 596, 647, 768, 959, 1084, 1220, 1298, 1329
NarI GGCGCC 1 cut(s) 1416
NcoI CCATGG 2 cut(s) 139, 1649
NdeII GATC 8 cut(s) 323, 482, 1282, 1474, 1551, 1749, 1782, 1948
NlaIII CATG 4 cut(s) 73, 143, 502, 1653
NlaIV GGNNCC 8 cut(s) 986, 1151, 1385, 1386, 1417, 1647, 1765, 2001
NmeAIII GCCGAG 5 cut(s) 806, 837, 1122, 1630, 2073
NmuCI GTSAC 2 cut(s) 364, 975
NruI TCGCGA 2 cut(s) 768, 1298
NspV TTCGAA 1 cut(s) 1337
OliI CACNNNNGTG 1 cut(s) 1993
PciSI GCTCTTC 1 cut(s) 1832
PcsI WCGNNNNNNNCGW 8 cut(s) 261, 692, 726, 971, 1326, 1467, 1743, 1970
PdmI GAANNNNTTC 1 cut(s) 1973
PfeI GAWTC 9 cut(s) 57, 246, 419, 917, 936, 1133, 1222, 1585, 1620
Pfl23II CGTACG 1 cut(s) 1911
PinAI ACCGGT 1 cut(s) 1990
PleI GAGTC 8 cut(s) 312, 551, 625, 757, 769, 791, 835, 1253
PluTI GGCGCC 1 cut(s) 1419
PmaCI CACGTG 1 cut(s) 1039
PmlI CACGTG 1 cut(s) 1039
PpsI GAGTC 8 cut(s) 312, 551, 625, 757, 769, 791, 835, 1253
Ppu21I YACGTR 4 cut(s) 541, 754, 1039, 1747
PpuMI RGGWCCY 1 cut(s) 1384
Psp124BI GAGCTC 3 cut(s) 581, 611, 738
Psp5II RGGWCCY 1 cut(s) 1384
Psp6I CCWGG 2 cut(s) 569, 1357
PspCI CACGTG 1 cut(s) 1039
PspFI CCCAGC 4 cut(s) 478, 512, 714, 816
PspGI CCWGG 2 cut(s) 569, 1357
PspLI CGTACG 1 cut(s) 1911
PspN4I GGNNCC 8 cut(s) 986, 1151, 1385, 1386, 1417, 1647, 1765, 2001
PspOMI GGGCCC 1 cut(s) 1645
PspPI GGNCC 9 cut(s) 143, 438, 457, 487, 1017, 1384, 1421, 1645, 1646
PspPPI RGGWCCY 1 cut(s) 1384
PstI CTGCAG 1 cut(s) 1632
PstNI CAGNNNCTG 1 cut(s) 1636
PvuII CAGCTG 2 cut(s) 131, 584
RruI TCGCGA 2 cut(s) 768, 1298
RsaI GTAC 6 cut(s) 593, 742, 1151, 1255, 1745, 1913
RsaNI GTAC 6 cut(s) 592, 741, 1150, 1254, 1744, 1912
RseI CAYNNNNRTG 3 cut(s) 752, 1001, 1993
Rsr2I CGGWCCG 1 cut(s) 487
RsrII CGGWCCG 1 cut(s) 487
SacI GAGCTC 3 cut(s) 581, 611, 738
SalI GTCGAC 1 cut(s) 1247
SapI GCTCTTC 1 cut(s) 1832
SaqAI TTAA 1 cut(s) 1172
Sau3AI GATC 8 cut(s) 323, 482, 1282, 1474, 1551, 1749, 1782, 1948
Sau96I GGNCC 9 cut(s) 143, 438, 457, 487, 1017, 1384, 1421, 1645, 1646
SchI GAGTC 8 cut(s) 312, 551, 625, 757, 769, 792, 836, 1254
SduI GDGCHC 6 cut(s) 581, 611, 738, 801, 1103, 1649
SfaNI GCATC 2 cut(s) 162, 1906
SfcI CTRYAG 1 cut(s) 1628
SfoI GGCGCC 1 cut(s) 1417
SfuI TTCGAA 1 cut(s) 1337
SgrAI CRCCGGYG 2 cut(s) 1412, 1990
SinI GGWCC 4 cut(s) 438, 457, 487, 1384
SmaI CCCGGG 1 cut(s) 982
SmiMI CAYNNNNRTG 3 cut(s) 752, 1001, 1993
SmlI CTYRAG 1 cut(s) 2125
SmoI CTYRAG 1 cut(s) 2125
Sse9I AATT 5 cut(s) 79, 84, 1427, 1969, 2083
SspDI GGCGCC 1 cut(s) 1415
SspMI CTAG 1 cut(s) 2149
SstI GAGCTC 3 cut(s) 581, 611, 738
StyI CCWWGG 3 cut(s) 139, 1649, 2117
TaaI ACNGT 4 cut(s) 1253, 1334, 1916, 1930
TaiI ACGT 9 cut(s) 366, 543, 756, 977, 1041, 1320, 1379, 1473, 1749
TaqII GACCGA 1 cut(s) 1560
TasI AATT 5 cut(s) 79, 84, 1427, 1969, 2083
TatI WGTACW 1 cut(s) 740
TauI GCSGC 6 cut(s) 692, 695, 807, 946, 1087, 2058
TfiI GAWTC 9 cut(s) 57, 246, 419, 917, 936, 1133, 1222, 1585, 1620
Tru1I TTAA 1 cut(s) 1172
Tru9I TTAA 1 cut(s) 1172
TscAI CASTG 5 cut(s) 31, 45, 869, 1450, 2144
TseFI GTSAC 2 cut(s) 364, 975
TseI GCWGC 6 cut(s) 109, 112, 584, 1627, 2052, 2144
Tsp45I GTSAC 2 cut(s) 364, 975
TspDTI ATGAA 8 cut(s) 17, 80, 86, 112, 515, 985, 1111, 1999
TspGWI ACGGA 9 cut(s) 253, 929, 1191, 1225, 1521, 1564, 1655, 1670, 1776
TspMI CCCGGG 1 cut(s) 980
TspRI CASTG 5 cut(s) 31, 45, 869, 1450, 2144
VpaK11BI GGWCC 4 cut(s) 438, 457, 487, 1384
XapI RAATTY 2 cut(s) 1427, 1969
XmaI CCCGGG 1 cut(s) 980
XmiI GTMKAC 5 cut(s) 537, 1248, 1314, 1353, 1407
XmnI GAANNNNTTC 1 cut(s) 1973
XspI CTAG 1 cut(s) 2149
ZraI GACGTC 1 cut(s) 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.