Rroxscaffold_7G00171530

WD40-like Beta Propeller Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016112
Physical Location & Seq
Forward (+)
11832976 .. 11835068
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00171530.1

Sequence Viewer

Length: 2034 bp
ATGAAACCCAATTCAATTCTCTATTTGTTGCTCGATTTGCTGGTGCTTAGTTTCAGTTTAGCGGCGGAACATGGCCCTTCCAGCATCGTCTTCACCACCAACGGCAGATTAGATTACCATTTCGACATCTTCACCCTCCCGATCGGTCACCCACCGCACCCAACCACCGAGACCCGAATCACAGATGGCCAGTCCGTCAACTTGAACGGCCACTTCCCCTCCTCCACTTTCTTTCCCAAACAAACCCGAATCCGATCCCCCACCCGACCCGACCCCTCTCTCCAGATCATCTACGTCACCGAGAGAAACGGCTTTTCCAACATCTACTACGACGCCGTTTACTTCGATTCTCCGTCGAGCATCAGGAAAAGGTCCGCCCTTGAAGTCGTGGACCGGGTTCAGGTCCCTCTGCTGGGCTTGGACGGGGTTTCGATGAAAGACAAGCCCAGTTTGACAGGGGAGTATTTGGTATACGTGTCCACTCACGAAGACTCCGGCGTGCCCAGGACGAGTTCTGCTGCGGTTTACTCGACCGAGTTGAACTCGGGTTTGACCCGGCGGCTGACTCCTTACGGCGTCGCGGACTTCAGCCCCGCGGTGTCGCCGTCTGGGTTTTGGACGGCGGCGGCGTCGTATGGGCCGAAAGGTTGGGACGGCGACGTCGAGGAGCTCAGTACAGACATCTACGTGTTCTTGACTCGGGACGGGACTCGCCGAGTCAAGAAGAGGAGAAGACCAGTGGTGGAGTATTTATCGGGCGATTTTACCGAGAGGGGGCCGACTTCTGCCGAGTCAGTGGTGGTGGAGCGAGTTACGCCGCCGGGTCTCCACGCGTTCACACCCGCCACGTCACCGGGGAACCACGACTTCATCGCCGTGGCCACAAGAAGACCCACCACGAGCTTCCGCCACGTGGAGCTGTTCGACTTGGCGAAGGGCGAGTTCAGGGAGCTGACTCGGCTCGTCGCGCCACGTACCCACCACTACAACCCGTTCATCTCCCCCGATTCGAGTCGGGTCGGGTACCACAAGTGCAGAGGCAACGCGAACGGGAAGAAAAGCCGGAAGCTTTTCCTTCAGAATATCCACAGCTCAATTCCGAACCTGTCCGTGCTCAGAATCGACGGCACGTTTCCTTCGTTTTCACCCGCTGGTGACCGTATCGCCTTCGCGAACTTACCGGGTATCTACGTGGTGAACCGCGACGGTTCGAACCGGCGACAGGTCTACCCTGGAAAAGCTTTCTCGACCGCCTGGGACCCGGTTCGCAAGGGCGTCATATACACCGGCGCCGGACCGGAATTTGCACCGGAGAGCGCTGAGGTTGATATCATCTCCATCGACGTTGACCAAATCGCTTTCAAGAAGTTGACCACAAACGGAAAGAACAACGCCTTCCCTTCACCCTCGCCCGACGGAAAGCGGATCGTCTTCCGGTCGGGTCTGTCGGGTCACAAAAACCTGTACATCATAGACGCCGAGGAAGGTGAGACACTTGGGCTCCAGAGACTGACGGAGGGTCCATGGACGGACACGATGTGTAACTGGTCGCCGGACGGCGACTGGATCGCGTTCGCGTCGGACCGGGAGAACCCGGGTAGCGGGAGCTTCGAGTTGTACGTGATCCACCCGAATGGAACCGGGTTAAGGAAAGTGATCCAAAGCGGGTCGGGTGGACGGACTAACCACCCGTGGTTCAGCCCGGATGGGAAGAGCTTGGTTTTCACTTCGGACTATGGGGGCATATCAGCTGAGCCCATCTCAAACCCGCATCATTACCAGCCGTACGGTGAGATTTTTACTGTGAAATTGGACGGCTCTGATGTTACGAGGTTGACACAGAATTCGTTTGAGGATGGAACACCGGTGTGGGTCCCCAGCTTCATCAGGCCGTCGGATGTAGAGTGGCCTATTGATAGGCCTAGCTGCAGCTTTGATGATTTGCACTGCCTCAGTGATTTTCCTAGCTATGGTCAGAATGGTGGTGTTGGGGGTGTTCCTTGGCTCTTAAGCAAGCCTCAATGTGGTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PD40

Protein Analysis

677

Amino Acids

74.52

Weight (kDa)

6.56

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tricorn_N PF26549 442 - 625 6.1e-20 Tricorn protease N-terminal beta propeller domain
Tricorn_2nd PF26550 443 - 578 1.3e-08 Tricorn protease second beta propeller domain
PD40 PF07676 455 - 489 5.7e-06 WD40-like Beta Propeller Repeat
Tricorn_2nd PF26550 470 - 578 2e-07 Tricorn protease second beta propeller domain
PD40 PF07676 502 - 541 9.3e-08 WD40-like Beta Propeller Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016112)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21680
fragaria_vesca FvH4_2g27620
malus_domestica MD15G1344100.v1.1
prunus_persica Prupe.1G491500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0296441
rosa_laevigata RLG00000011693
rosa_multiflora Rmu_sc0028875.1_g000001
rosa_roxburghii Rroxscaffold_7G00171530 Rroxscaffold_7G00178600
rosa_rugosa Rorug06G0262400
rosa_samantha Rh6AG373600 Rh6BG382400 Rh6CG387200 Rh6DG374900
rosa_wichuraiana Rw6G032600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 659
AatII GACGTC 1 cut(s) 663
Acc65I GGTACC 1 cut(s) 1023
AccB1I GGYRCC 2 cut(s) 1023, 1289
AccI GTMKAC 2 cut(s) 471, 1227
AccII CGCG 9 cut(s) 581, 596, 833, 968, 1046, 1172, 1203, 1571, 1577
AclWI GGATC 5 cut(s) 249, 1433, 1574, 1618, 1651
AcoI YGGCCR 3 cut(s) 187, 208, 879
AcsI RAATTY 2 cut(s) 1301, 1843
AcuI CTGAAG 2 cut(s) 571, 1061
AcvI CACGTG 1 cut(s) 913
AcyI GRCGYC 7 cut(s) 333, 576, 629, 660, 1275, 1290, 1476
AdeI CACNNNGTG 1 cut(s) 1539
AfaI GTAC 6 cut(s) 676, 976, 1025, 1466, 1619, 1787
AfeI AGCGCT 1 cut(s) 1318
AflII CTTAAG 1 cut(s) 2008
AflIII ACRYGT 3 cut(s) 474, 687, 831
AgeI ACCGGT 1 cut(s) 1864
AgsI TTSAA 5 cut(s) 15, 205, 383, 541, 1363
AhdI GACNNNNNGTC 1 cut(s) 1518
AjiI CACGTC 1 cut(s) 849
AjnI CCWGG 3 cut(s) 503, 1231, 1253
AleI CACNNNNGTG 1 cut(s) 1867
Alw21I GWGCWC 2 cut(s) 672, 1116
Alw26I GTCTC 4 cut(s) 164, 830, 1484, 1501
AlwI GGATC 5 cut(s) 249, 1433, 1574, 1618, 1651
AlwNI CAGNNNCTG 1 cut(s) 1510
Ama87I CYCGRG 3 cut(s) 544, 699, 1594
Aor51HI AGCGCT 1 cut(s) 1318
AoxI GGCC 9 cut(s) 73, 187, 208, 638, 776, 879, 1889, 1907, 1919
ApeKI GCWGC 3 cut(s) 518, 1926, 1929
ApoI RAATTY 2 cut(s) 1301, 1843
ArsI GACNNNNNNTTYG 2 cut(s) 1828, 1860
AsiGI ACCGGT 1 cut(s) 1864
Asp700I GAANNNNTTC 1 cut(s) 1070
Asp718I GGTACC 1 cut(s) 1023
AspLEI GCGC 3 cut(s) 970, 1292, 1319
AsuII TTCGAA 1 cut(s) 1211
AvaI CYCGRG 3 cut(s) 544, 699, 1594
AvaII GGWCC 8 cut(s) 372, 391, 403, 1258, 1295, 1520, 1582, 1873
BaeGI GKGCMC 1 cut(s) 504
BalI TGGCCA 2 cut(s) 189, 881
BanI GGYRCC 2 cut(s) 1023, 1289
BanII GRGCYC 3 cut(s) 672, 1503, 1758
BauI CACGAG 1 cut(s) 898
BbrPI CACGTG 1 cut(s) 913
BbsI GAAGAC 5 cut(s) 82, 495, 739, 895, 1423
Bbv12I GWGCWC 2 cut(s) 672, 1116
BbvCI CCTCAGC 1 cut(s) 1320
BbvI GCAGC 3 cut(s) 505, 1913, 1941
BccI CCATC 5 cut(s) 179, 1346, 1700, 1766, 1850
BciT130I CCWGG 3 cut(s) 505, 1233, 1255
BcoDI GTCTC 4 cut(s) 164, 830, 1484, 1501
BfaI CTAG 2 cut(s) 1923, 1965
BfmI CTRYAG 1 cut(s) 1927
BfoI RGCGCY 2 cut(s) 1293, 1320
BfrI CTTAAG 1 cut(s) 2008
BisI GCNGC 8 cut(s) 63, 519, 560, 624, 627, 818, 1927, 1930
BlpI GCTNAGC 1 cut(s) 1752
BlsI GCNGC 8 cut(s) 64, 520, 561, 625, 628, 819, 1928, 1931
Bme18I GGWCC 8 cut(s) 372, 391, 403, 1258, 1295, 1520, 1582, 1873
BmeRI GACNNNNNGTC 1 cut(s) 1518
BmeT110I CYCGRG 3 cut(s) 544, 699, 1594
BmgBI CACGTC 1 cut(s) 849
BmrI ACTGGG 1 cut(s) 441
BmsI GCATC 3 cut(s) 93, 369, 1780
BmuI ACTGGG 1 cut(s) 441
BpiI GAAGAC 5 cut(s) 82, 495, 739, 895, 1423
BplI GAGNNNNNCTC 4 cut(s) 527, 559, 1745, 1777
BpmI CTGGAG 2 cut(s) 266, 1487
Bpu10I CCTNAGC 1 cut(s) 1320
Bpu1102I GCTNAGC 1 cut(s) 1752
Bpu14I TTCGAA 1 cut(s) 1211
BsaAI YACGTR 6 cut(s) 475, 688, 913, 974, 1192, 1621
BsaHI GRCGYC 7 cut(s) 333, 576, 629, 660, 1275, 1290, 1476
BsaI GGTCTC 2 cut(s) 164, 830
BsaWI WCCGGW 4 cut(s) 1297, 1309, 1434, 1864
BsaXI ACNNNNNCTCC 6 cut(s) 203, 233, 476, 506, 1485, 1515
Bse118I RCCGGY 3 cut(s) 1215, 1286, 1864
Bse1I ACTGG 5 cut(s) 190, 447, 737, 1550, 1568
BseBI CCWGG 3 cut(s) 505, 1233, 1255
BseGI GGATG 3 cut(s) 1711, 1861, 1903
BseMII CTCAG 5 cut(s) 685, 1129, 1311, 1743, 1966
BseNI ACTGG 5 cut(s) 190, 447, 737, 1550, 1568
BseRI GAGGAG 3 cut(s) 211, 680, 742
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 3 cut(s) 505, 1913, 1941
BseYI CCCAGC 2 cut(s) 412, 1877
BsgI GTGCAG 1 cut(s) 1054
Bsh1236I CGCG 9 cut(s) 581, 596, 833, 968, 1046, 1172, 1203, 1571, 1577
Bsh1285I CGRYCG 4 cut(s) 144, 534, 1251, 1439
BshFI GGCC 9 cut(s) 75, 189, 210, 640, 778, 881, 1891, 1909, 1921
BshNI GGYRCC 2 cut(s) 1023, 1289
BshTI ACCGGT 1 cut(s) 1864
BsiEI CGRYCG 4 cut(s) 144, 534, 1251, 1439
BsiHKAI GWGCWC 2 cut(s) 672, 1116
BsiHKCI CYCGRG 3 cut(s) 544, 699, 1594
BsiWI CGTACG 1 cut(s) 1785
BslFI GGGAC 6 cut(s) 389, 665, 716, 721, 1271, 1859
BsmAI GTCTC 4 cut(s) 164, 830, 1484, 1501
BsmFI GGGAC 6 cut(s) 389, 665, 716, 721, 1271, 1859
BsnI GGCC 9 cut(s) 75, 189, 210, 640, 778, 881, 1891, 1909, 1921
Bso31I GGTCTC 2 cut(s) 164, 830
BsoBI CYCGRG 3 cut(s) 544, 699, 1594
Bsp119I TTCGAA 1 cut(s) 1211
Bsp1286I GDGCHC 5 cut(s) 504, 672, 1116, 1503, 1758
Bsp1407I TGTACA 1 cut(s) 1464
Bsp143I GATC 7 cut(s) 141, 254, 285, 1425, 1566, 1623, 1656
Bsp1720I GCTNAGC 1 cut(s) 1752
Bsp19I CCATGG 1 cut(s) 1523
Bsp68I TCGCGA 1 cut(s) 1172
BspANI GGCC 9 cut(s) 75, 189, 210, 640, 778, 881, 1891, 1909, 1921
BspCNI CTCAG 5 cut(s) 684, 1128, 1312, 1744, 1965
BspFNI CGCG 9 cut(s) 581, 596, 833, 968, 1046, 1172, 1203, 1571, 1577
BspMAI CTGCAG 1 cut(s) 1931
BspPI GGATC 5 cut(s) 249, 1433, 1574, 1618, 1651
BspQI GCTCTTC 1 cut(s) 1706
BspT104I TTCGAA 1 cut(s) 1211
BspT107I GGYRCC 2 cut(s) 1023, 1289
BspTI CTTAAG 1 cut(s) 2008
BspTNI GGTCTC 2 cut(s) 164, 830
BsrFI RCCGGY 3 cut(s) 1215, 1286, 1864
BsrGI TGTACA 1 cut(s) 1464
BsrI ACTGG 5 cut(s) 190, 447, 737, 1550, 1568
BssAI RCCGGY 3 cut(s) 1215, 1286, 1864
BssMI GATC 7 cut(s) 141, 254, 285, 1425, 1566, 1623, 1656
BssNAI GTATAC 1 cut(s) 472
BssNI GRCGYC 7 cut(s) 333, 576, 629, 660, 1275, 1290, 1476
BssSI CACGAG 1 cut(s) 898
BssT1I CCWWGG 2 cut(s) 1523, 2000
Bst1107I GTATAC 1 cut(s) 472
Bst2BI CACGAG 1 cut(s) 898
Bst2UI CCWGG 3 cut(s) 505, 1233, 1255
Bst4CI ACNGT 4 cut(s) 1160, 1208, 1790, 1804
Bst6I CTCTTC 2 cut(s) 719, 1706
BstACI GRCGYC 7 cut(s) 333, 576, 629, 660, 1275, 1290, 1476
BstAFI CTTAAG 1 cut(s) 2008
BstAUI TGTACA 1 cut(s) 1464
BstBAI YACGTR 6 cut(s) 475, 688, 913, 974, 1192, 1621
BstBI TTCGAA 1 cut(s) 1211
BstC8I GCNNGC 2 cut(s) 500, 2015
BstDEI CTNAG 6 cut(s) 47, 671, 1115, 1320, 1752, 1952
BstDSI CCRYGG 4 cut(s) 594, 876, 1523, 1691
BstEII GGTNACC 2 cut(s) 146, 1154
BstF5I GGATG 3 cut(s) 1711, 1861, 1903
BstFNI CGCG 9 cut(s) 581, 596, 833, 968, 1046, 1172, 1203, 1571, 1577
BstH2I RGCGCY 2 cut(s) 1293, 1320
BstHHI GCGC 3 cut(s) 970, 1292, 1319
BstKTI GATC 7 cut(s) 144, 257, 288, 1428, 1569, 1626, 1659
BstMAI GTCTC 4 cut(s) 164, 830, 1484, 1501
BstMBI GATC 7 cut(s) 141, 254, 285, 1425, 1566, 1623, 1656
BstMCI CGRYCG 4 cut(s) 144, 534, 1251, 1439
BstMWI GCNNNNNNNGC 5 cut(s) 37, 81, 814, 958, 967
BstNI CCWGG 3 cut(s) 505, 1233, 1255
BstPI GGTNACC 2 cut(s) 146, 1154
BstSFI CTRYAG 1 cut(s) 1927
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 9 cut(s) 581, 596, 833, 968, 1046, 1172, 1203, 1571, 1577
BstV1I GCAGC 3 cut(s) 505, 1913, 1941
BstV2I GAAGAC 5 cut(s) 82, 495, 739, 895, 1423
BstXI CCANNNNNNTGG 1 cut(s) 1634
BstZ17I GTATAC 1 cut(s) 472
BsuRI GGCC 9 cut(s) 75, 189, 210, 640, 778, 881, 1891, 1909, 1921
BtgI CCRYGG 4 cut(s) 594, 876, 1523, 1691
BtgZI GCGATG 1 cut(s) 856
BtrI CACGTC 1 cut(s) 849
BtsCI GGATG 3 cut(s) 1711, 1861, 1903
BtsI GCAGTG 1 cut(s) 1945
BtsIMutI CAGTG 4 cut(s) 744, 801, 1945, 1960
BtuMI TCGCGA 1 cut(s) 1172
Cac8I GCNNGC 2 cut(s) 500, 2015
CaiI CAGNNNCTG 1 cut(s) 1510
CfoI GCGC 3 cut(s) 970, 1292, 1319
Cfr10I RCCGGY 3 cut(s) 1215, 1286, 1864
Cfr42I CCGCGG 1 cut(s) 597
Cfr9I CCCGGG 1 cut(s) 1594
CpoI CGGWCCG 2 cut(s) 1295, 1582
CseI GACGC 6 cut(s) 341, 565, 618, 1264, 1484, 1566
Csp6I GTAC 6 cut(s) 675, 975, 1024, 1465, 1618, 1786
CspAI ACCGGT 1 cut(s) 1864
CspI CGGWCCG 2 cut(s) 1295, 1582
CviAII CATG 2 cut(s) 71, 1524
CviQI GTAC 6 cut(s) 675, 975, 1024, 1465, 1618, 1786
DdeI CTNAG 6 cut(s) 47, 671, 1115, 1320, 1752, 1952
DinI GGCGCC 1 cut(s) 1291
DpnI GATC 7 cut(s) 143, 256, 287, 1427, 1568, 1625, 1658
DpnII GATC 7 cut(s) 141, 254, 285, 1425, 1566, 1623, 1656
DraIII CACNNNGTG 1 cut(s) 1539
DrdI GACNNNNNNGTC 1 cut(s) 659
DriI GACNNNNNGTC 1 cut(s) 1518
DseDI GACNNNNNNGTC 1 cut(s) 659
EaeI YGGCCR 3 cut(s) 187, 208, 879
Eam1104I CTCTTC 2 cut(s) 719, 1706
Eam1105I GACNNNNNGTC 1 cut(s) 1518
EarI CTCTTC 2 cut(s) 719, 1706
EciI GGCGGA 3 cut(s) 80, 364, 896
Ecl136II GAGCTC 1 cut(s) 670
Eco130I CCWWGG 2 cut(s) 1523, 2000
Eco147I AGGCCT 1 cut(s) 1921
Eco24I GRGCYC 3 cut(s) 672, 1503, 1758
Eco31I GGTCTC 2 cut(s) 164, 830
Eco32I GATATC 1 cut(s) 1330
Eco47I GGWCC 8 cut(s) 372, 391, 403, 1258, 1295, 1520, 1582, 1873
Eco47III AGCGCT 1 cut(s) 1318
Eco53kI GAGCTC 1 cut(s) 670
Eco57I CTGAAG 2 cut(s) 571, 1061
Eco72I CACGTG 1 cut(s) 913
Eco88I CYCGRG 3 cut(s) 544, 699, 1594
Eco91I GGTNACC 2 cut(s) 146, 1154
EcoICRI GAGCTC 1 cut(s) 670
EcoO109I RGGNCCY 3 cut(s) 403, 1258, 1873
EcoO65I GGTNACC 2 cut(s) 146, 1154
EcoRI GAATTC 1 cut(s) 1843
EcoRII CCWGG 3 cut(s) 503, 1231, 1253
EcoRV GATATC 1 cut(s) 1330
EcoT14I CCWWGG 2 cut(s) 1523, 2000
EcoT38I GRGCYC 3 cut(s) 672, 1503, 1758
EgeI GGCGCC 1 cut(s) 1291
EheI GGCGCC 1 cut(s) 1291
ErhI CCWWGG 2 cut(s) 1523, 2000
FaeI CATG 2 cut(s) 74, 1527
FaqI GGGAC 6 cut(s) 389, 665, 716, 721, 1271, 1859
FatI CATG 2 cut(s) 70, 1523
FauI CCCGC 6 cut(s) 601, 850, 1156, 1595, 1658, 1776
FblI GTMKAC 2 cut(s) 471, 1227
Fnu4HI GCNGC 8 cut(s) 63, 519, 560, 624, 627, 818, 1927, 1930
FokI GGATG 3 cut(s) 1718, 1868, 1910
FriOI GRGCYC 3 cut(s) 672, 1503, 1758
Fsp4HI GCNGC 8 cut(s) 63, 519, 560, 624, 627, 818, 1927, 1930
FspBI CTAG 2 cut(s) 1923, 1965
GlaI GCGC 3 cut(s) 969, 1291, 1318
GluI GCNGC 8 cut(s) 63, 519, 560, 624, 627, 818, 1927, 1930
GsaI CCCAGC 2 cut(s) 416, 1881
GsuI CTGGAG 2 cut(s) 266, 1487
HaeII RGCGCY 2 cut(s) 1293, 1320
HaeIII GGCC 9 cut(s) 75, 189, 210, 640, 778, 881, 1891, 1909, 1921
HgaI GACGC 6 cut(s) 341, 565, 618, 1264, 1484, 1566
HhaI GCGC 3 cut(s) 970, 1292, 1319
Hin1I GRCGYC 7 cut(s) 333, 576, 629, 660, 1275, 1290, 1476
Hin1II CATG 2 cut(s) 74, 1527
Hin6I GCGC 3 cut(s) 968, 1290, 1317
HinP1I GCGC 3 cut(s) 968, 1290, 1317
HincII GTYRAC 4 cut(s) 199, 1348, 1371, 1836
HindII GTYRAC 4 cut(s) 199, 1348, 1371, 1836
HindIII AAGCTT 2 cut(s) 1067, 1239
Hpy188I TCNGA 9 cut(s) 254, 1080, 1101, 1118, 1582, 1732, 1822, 1897, 1977
HpyAV CCTTC 8 cut(s) 87, 928, 1085, 1146, 1177, 1405, 1410, 1478
HpyCH4III ACNGT 4 cut(s) 1160, 1208, 1790, 1804
HpyCH4V TGCA 5 cut(s) 1035, 1307, 1929, 1945, 2030
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 81, 814, 958, 967
HpyF3I CTNAG 6 cut(s) 47, 671, 1115, 1320, 1752, 1952
Hsp92I GRCGYC 7 cut(s) 333, 576, 629, 660, 1275, 1290, 1476
Hsp92II CATG 2 cut(s) 74, 1527
HspAI GCGC 3 cut(s) 968, 1290, 1317
KasI GGCGCC 1 cut(s) 1289
KflI GGGWCCC 2 cut(s) 1258, 1873
KpnI GGTACC 1 cut(s) 1027
KspI CCGCGG 1 cut(s) 597
Kzo9I GATC 7 cut(s) 141, 254, 285, 1425, 1566, 1623, 1656
LguI GCTCTTC 1 cut(s) 1706
LmnI GCTCC 6 cut(s) 667, 805, 916, 949, 1506, 1605
Lsp1109I GCAGC 3 cut(s) 505, 1913, 1941
LweI GCATC 3 cut(s) 93, 369, 1780
MaeI CTAG 2 cut(s) 1923, 1965
MaeIII GTNAC 8 cut(s) 146, 295, 811, 849, 1154, 1451, 1541, 1825
MalI GATC 7 cut(s) 143, 256, 287, 1427, 1568, 1625, 1658
MboI GATC 7 cut(s) 141, 254, 285, 1425, 1566, 1623, 1656
MboII GAAGA 9 cut(s) 82, 121, 500, 736, 744, 900, 1066, 1423, 1723
MhlI GDGCHC 5 cut(s) 504, 672, 1116, 1503, 1758
MlsI TGGCCA 2 cut(s) 189, 881
MluCI AATT 6 cut(s) 10, 15, 1095, 1301, 1808, 1843
MluI ACGCGT 1 cut(s) 831
MluNI TGGCCA 2 cut(s) 189, 881
Mly113I GGCGCC 1 cut(s) 1290
MlyI GAGTC 8 cut(s) 485, 559, 691, 703, 726, 800, 949, 1021
MmeI TCCRAC 3 cut(s) 342, 1560, 1875
Mox20I TGGCCA 2 cut(s) 189, 881
MroXI GAANNNNTTC 1 cut(s) 1070
MscI TGGCCA 2 cut(s) 189, 881
MseI TTAA 2 cut(s) 1646, 2009
MslI CAYNNNNRTG 4 cut(s) 686, 875, 1632, 1867
Msp20I TGGCCA 2 cut(s) 189, 881
MspA1I CMGCKG 3 cut(s) 596, 1151, 1751
MspCI CTTAAG 1 cut(s) 2008
MvaI CCWGG 3 cut(s) 505, 1233, 1255
MvnI CGCG 9 cut(s) 581, 596, 833, 968, 1046, 1172, 1203, 1571, 1577
MwoI GCNNNNNNNGC 5 cut(s) 37, 81, 814, 958, 967
NarI GGCGCC 1 cut(s) 1290
NcoI CCATGG 1 cut(s) 1523
NdeII GATC 7 cut(s) 141, 254, 285, 1425, 1566, 1623, 1656
NlaIII CATG 2 cut(s) 74, 1527
NmeAIII GCCGAG 4 cut(s) 740, 814, 937, 1504
NmuCI GTSAC 5 cut(s) 146, 295, 849, 1154, 1451
NruI TCGCGA 1 cut(s) 1172
NspV TTCGAA 1 cut(s) 1211
OliI CACNNNNGTG 1 cut(s) 1867
PceI AGGCCT 1 cut(s) 1921
PciSI GCTCTTC 1 cut(s) 1706
PcsI WCGNNNNNNNCGW 5 cut(s) 626, 638, 660, 1136, 1617
PdmI GAANNNNTTC 1 cut(s) 1070
PfeI GAWTC 5 cut(s) 177, 249, 347, 1007, 1119
Pfl23II CGTACG 1 cut(s) 1785
PinAI ACCGGT 1 cut(s) 1864
PkrI GCNGC 8 cut(s) 64, 520, 561, 625, 628, 819, 1928, 1931
Ple19I CGATCG 1 cut(s) 144
PleI GAGTC 8 cut(s) 485, 559, 691, 703, 725, 799, 949, 1020
PluTI GGCGCC 1 cut(s) 1293
PmaCI CACGTG 1 cut(s) 913
PmlI CACGTG 1 cut(s) 913
PpsI GAGTC 8 cut(s) 485, 559, 691, 703, 725, 799, 949, 1020
Ppu21I YACGTR 6 cut(s) 475, 688, 913, 974, 1192, 1621
PpuMI RGGWCCY 3 cut(s) 403, 1258, 1873
Psp124BI GAGCTC 1 cut(s) 672
Psp5II RGGWCCY 3 cut(s) 403, 1258, 1873
Psp6I CCWGG 3 cut(s) 503, 1231, 1253
PspCI CACGTG 1 cut(s) 913
PspEI GGTNACC 2 cut(s) 146, 1154
PspFI CCCAGC 2 cut(s) 412, 1877
PspGI CCWGG 3 cut(s) 503, 1231, 1253
PspLI CGTACG 1 cut(s) 1785
PspPPI RGGWCCY 3 cut(s) 403, 1258, 1873
PstI CTGCAG 1 cut(s) 1931
PstNI CAGNNNCTG 1 cut(s) 1510
PvuI CGATCG 1 cut(s) 144
PvuII CAGCTG 1 cut(s) 1751
RruI TCGCGA 1 cut(s) 1172
RsaI GTAC 6 cut(s) 676, 976, 1025, 1466, 1619, 1787
RsaNI GTAC 6 cut(s) 675, 975, 1024, 1465, 1618, 1786
RseI CAYNNNNRTG 4 cut(s) 686, 875, 1632, 1867
Rsr2I CGGWCCG 2 cut(s) 1295, 1582
RsrII CGGWCCG 2 cut(s) 1295, 1582
SacI GAGCTC 1 cut(s) 672
SacII CCGCGG 1 cut(s) 597
SapI GCTCTTC 1 cut(s) 1706
SaqAI TTAA 2 cut(s) 1646, 2009
SatI GCNGC 8 cut(s) 63, 519, 560, 624, 627, 818, 1927, 1930
Sau3AI GATC 7 cut(s) 141, 254, 285, 1425, 1566, 1623, 1656
SchI GAGTC 8 cut(s) 485, 559, 691, 703, 726, 800, 949, 1021
SduI GDGCHC 5 cut(s) 504, 672, 1116, 1503, 1758
SfaNI GCATC 3 cut(s) 93, 369, 1780
SfcI CTRYAG 1 cut(s) 1927
SfoI GGCGCC 1 cut(s) 1291
Sfr303I CCGCGG 1 cut(s) 597
SfuI TTCGAA 1 cut(s) 1211
SgrAI CRCCGGYG 2 cut(s) 1286, 1864
SgrBI CCGCGG 1 cut(s) 597
SinI GGWCC 8 cut(s) 372, 391, 403, 1258, 1295, 1520, 1582, 1873
SmaI CCCGGG 1 cut(s) 1596
SmiMI CAYNNNNRTG 4 cut(s) 686, 875, 1632, 1867
SmlI CTYRAG 1 cut(s) 2008
SmoI CTYRAG 1 cut(s) 2008
Sse9I AATT 6 cut(s) 10, 15, 1095, 1301, 1808, 1843
SseBI AGGCCT 1 cut(s) 1921
SspDI GGCGCC 1 cut(s) 1289
SspMI CTAG 2 cut(s) 1923, 1965
SstI GAGCTC 1 cut(s) 672
StuI AGGCCT 1 cut(s) 1921
StyI CCWWGG 2 cut(s) 1523, 2000
TaaI ACNGT 4 cut(s) 1160, 1208, 1790, 1804
TaqII GACCGA 2 cut(s) 134, 548
TasI AATT 6 cut(s) 10, 15, 1095, 1301, 1808, 1843
TatI WGTACW 2 cut(s) 674, 1464
TauI GCSGC 5 cut(s) 65, 562, 626, 629, 820
TfiI GAWTC 5 cut(s) 177, 249, 347, 1007, 1119
Tru1I TTAA 2 cut(s) 1646, 2009
Tru9I TTAA 2 cut(s) 1646, 2009
TscAI CASTG 4 cut(s) 744, 801, 1952, 1960
TseFI GTSAC 5 cut(s) 146, 295, 849, 1154, 1451
TseI GCWGC 3 cut(s) 518, 1926, 1929
Tsp45I GTSAC 5 cut(s) 146, 295, 849, 1154, 1451
TspDTI ATGAA 5 cut(s) 17, 449, 859, 985, 1873
TspGWI ACGGA 8 cut(s) 184, 342, 1099, 1395, 1431, 1529, 1544, 1693
TspMI CCCGGG 1 cut(s) 1594
TspRI CASTG 4 cut(s) 744, 801, 1952, 1960
Vha464I CTTAAG 1 cut(s) 2008
VpaK11BI GGWCC 8 cut(s) 372, 391, 403, 1258, 1295, 1520, 1582, 1873
XapI RAATTY 2 cut(s) 1301, 1843
XmaI CCCGGG 1 cut(s) 1594
XmiI GTMKAC 2 cut(s) 471, 1227
XmnI GAANNNNTTC 1 cut(s) 1070
XspI CTAG 2 cut(s) 1923, 1965
ZraI GACGTC 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.