Rh6DG374900

WD40-like Beta Propeller Repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0016112
Physical Location & Seq
Reverse (-)
56948242 .. 56951309
3068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG374900.1

Sequence Viewer

Length: 2088 bp
ATGAAACCCAATTCAATTCTCCATTTGTTGCTCCATTTGCTGGTGCTTAGTTTCAGTTTAGCGGCTGAACATGGCCCTTCCAGCATCGTCTTCACCACCAACGGCAGATTAGATTACTATTTCGACATCTTCACCCTCCCGATCAGTCACCCAACCACCGAGACCCGAATCACAGATGGCCAGTCCGTCAACTTCAACGGCCACTTCCCCTCCTCCACTTTCTTTCCCAACCAAACCCGAATCCGATCCCCCACCCGACCCGACCCCTCTCTCCAGATCATCTACGTCACCGAGAGAAACGGCTTTTCCAACATCTACTACGACGCCGTTTACTTCGATTCTCCGTCGAGCATCAGGAAAAGGTCCGCCCTTGAAGTCGTGGACCGGGTTCAGGTCCCTCTGCTGGGCTTGGACGGGGTTTCGATGAAAGACAAGCCCAGTTTGACAGGGGAGTATTTGGTATACGTGTCCACTCACGAAGACTCCGGCGTGCCCAGGACGAGTTCTGCTGCGGTTTACTCGACCGAGTTGAACTCGGCTTTGACCCGGAGGCTGACTCCTTACGGCGTCGCGGACTTCAGCCCCGCGGTGTCGCCGTCTGGGGTTTGGACGGCGGTGGCGTCGTATGGGTTGGAAGGTTGGGACGGCGAGGTCGAGGAGCTCAGTACAGACATCTACGTGTTCTTGACTCGGGACGGGACTCGCCGAGTCAAGGTGGTTGAACACGGCGGCTGGCCGAGTTGGGTTGACGAGTCAACGATTTACTTTCACAGAAGAGGAGAAGACCAGTGGTGGAGTATTTATCGGGCGATTTTACCGAGAGGGGGAGCGATTTCTACCGAGTCAATCGTGGTGGAGCGAGTTACGCCGCCGGGTCTCCACGCGTTCACACCCGCCACGTCACCGGGGAACCACGACTTCATCGCCGTGGCCACCAGAAGACCCACCACGAGCTTCCGCCACGTGGAGCTGTTCGACTTGGTGAAGGGCGAGTTCATGGAGCTGACTCGGCTCCTCGCGCCACGTACCCACCACTACAACCCGTTCATCTCCCCCGATTCGAGTCGGGTCGGGTACCACAAGTGCAGAGGCAACGCGAACGGGAAGAAAAGCCCGAAGCTTTTTCTTCAGAATATCCACAGCTCAATTCCGAACCTGTCCGTGCTCAGAATCGACGGTACGTTTCCTTCTTTTTCACCAGCAGGTGACGACCGTATCGCCTTCGCGAACTTACCGGGTATCTACGTGGTGAACCGCGACGGTTCGAACCGGCGACAGGTCTACCCTGGAAAAGCTTTCTCGACCGCCTGGGACCCGGTTCGCAAGGGCGTCATATACACCGGTGCCGGACCGGAATTTGCACCGGAGAGCGCTGAGGTTGATATCATCTCCATCGACGTTGACCGAATCGCCATCAAGAAGTTAACCACAAACGGGAAGAACAACGCCTTCCCTTCACCATCGCCCGACGGAAAACGGATCGTCTTCCGGTCGGGTCGGTCGGGTCATAAGAACCTCTACATCATGGACGCCGAGGAAGGTGAGACACTTGGGCTCCAGAGACTGACGGAGGGTCCATGGACGGACACGATGTGTAACTGGTCGCCGGACGGTGACTGGATCGCGTTCGCGTCGGACCGGGAGAACCCGGGCAGCGGGAGCTTCGAGTTGTACGTGATCCACCCGAACGGAACCGGGTTGAGGAAAGTGATCGAAAGCGGGTCGGGTGGACGGACCAACCACCCGTGGTTCAGCCCGGATGGGAAGAGCTTGGTTTTCACTTCGGATTATGGGGGCATATCAGCTGAGCCCATCTCAAACCCGCATCATTACCAGCCGTACGGTGAGATTTTTACTGTGAAATTGGACGGGTCTGATGTTACGAGGTTGACACAGAATTCGTTTGAGGATGGAACACCGGTGTGGGTCCCCAGCTTCATCAGGCCGTCGGATGTAGAGTGGCCTATTGATAGGCCTAGCTGCAGCTTTGATGATTTGCACTGCCTCAGTGATTTTCCTAGCTATGGTCAGAATGGTGGTGTTGGGGCTGTTCCTTGGCTCTTAAGCAAGCCTCAATGTGGTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PD40

Protein Analysis

695

Amino Acids

76.83

Weight (kDa)

6.04

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tricorn_N PF26549 461 - 643 1.2e-20 Tricorn protease N-terminal beta propeller domain
Tricorn_2nd PF26550 465 - 596 4.6e-09 Tricorn protease second beta propeller domain
PD40 PF07676 473 - 508 9.7e-08 WD40-like Beta Propeller Repeat
PD40 PF07676 520 - 559 9.6e-08 WD40-like Beta Propeller Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016112)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21680
fragaria_vesca FvH4_2g27620
malus_domestica MD15G1344100.v1.1
prunus_persica Prupe.1G491500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0296441
rosa_laevigata RLG00000011693
rosa_multiflora Rmu_sc0028875.1_g000001
rosa_roxburghii Rroxscaffold_7G00171530 Rroxscaffold_7G00178600
rosa_rugosa Rorug06G0262400
rosa_samantha Rh6AG373600 Rh6BG382400 Rh6CG387200 Rh6DG374900
rosa_wichuraiana Rw6G032600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1193
AasI GACNNNNNNGTC 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 1193
Acc65I GGTACC 1 cut(s) 1074
AccB1I GGYRCC 2 cut(s) 1074, 1343
AccB7I CCANNNNNTGG 1 cut(s) 40
AccI GTMKAC 2 cut(s) 462, 1281
AccII CGCG 9 cut(s) 572, 587, 884, 1019, 1097, 1226, 1257, 1625, 1631
AclWI GGATC 4 cut(s) 240, 1487, 1628, 1672
AcoI YGGCCR 4 cut(s) 178, 199, 734, 930
AcsI RAATTY 2 cut(s) 1355, 1897
AcuI CTGAAG 2 cut(s) 562, 1112
AcvI CACGTG 1 cut(s) 964
AcyI GRCGYC 5 cut(s) 324, 567, 620, 1329, 1530
AdeI CACNNNGTG 1 cut(s) 1593
AfaI GTAC 6 cut(s) 667, 1027, 1076, 1180, 1673, 1841
AfeI AGCGCT 1 cut(s) 1372
AflII CTTAAG 1 cut(s) 2062
AflIII ACRYGT 3 cut(s) 465, 678, 882
AgeI ACCGGT 2 cut(s) 1340, 1918
AgsI TTSAA 5 cut(s) 15, 196, 374, 532, 722
AhdI GACNNNNNGTC 1 cut(s) 1572
AjiI CACGTC 1 cut(s) 900
AjnI CCWGG 3 cut(s) 494, 1285, 1307
AleI CACNNNNGTG 1 cut(s) 1921
Alw21I GWGCWC 2 cut(s) 663, 1167
Alw26I GTCTC 4 cut(s) 155, 881, 1538, 1555
AlwI GGATC 4 cut(s) 240, 1487, 1628, 1672
AlwNI CAGNNNCTG 1 cut(s) 1564
Ama87I CYCGRG 2 cut(s) 690, 1648
Aor51HI AGCGCT 1 cut(s) 1372
AoxI GGCC 8 cut(s) 73, 178, 199, 734, 930, 1943, 1961, 1973
ApeKI GCWGC 4 cut(s) 509, 1653, 1980, 1983
ApoI RAATTY 2 cut(s) 1355, 1897
ArsI GACNNNNNNTTYG 2 cut(s) 1882, 1914
AsiGI ACCGGT 2 cut(s) 1340, 1918
Asp718I GGTACC 1 cut(s) 1074
AspLEI GCGC 2 cut(s) 1021, 1373
AsuII TTCGAA 1 cut(s) 1265
AvaI CYCGRG 2 cut(s) 690, 1648
AvaII GGWCC 9 cut(s) 363, 382, 394, 1312, 1349, 1574, 1636, 1734, 1927
BaeGI GKGCMC 1 cut(s) 495
BaeI ACNNNNGTAYC 2 cut(s) 1198, 1231
BalI TGGCCA 2 cut(s) 180, 932
BanI GGYRCC 2 cut(s) 1074, 1343
BanII GRGCYC 3 cut(s) 663, 1557, 1812
BarI GAAGNNNNNNTAC 2 cut(s) 1171, 1203
BauI CACGAG 1 cut(s) 949
BbrPI CACGTG 1 cut(s) 964
BbsI GAAGAC 5 cut(s) 82, 486, 789, 946, 1477
Bbv12I GWGCWC 2 cut(s) 663, 1167
BbvCI CCTCAGC 1 cut(s) 1374
BbvI GCAGC 4 cut(s) 496, 1665, 1967, 1995
BccI CCATC 7 cut(s) 170, 1400, 1421, 1468, 1754, 1820, 1904
BciT130I CCWGG 3 cut(s) 496, 1287, 1309
BcoDI GTCTC 4 cut(s) 155, 881, 1538, 1555
BfaI CTAG 2 cut(s) 1977, 2019
BfmI CTRYAG 1 cut(s) 1981
BfoI RGCGCY 1 cut(s) 1374
BfrI CTTAAG 1 cut(s) 2062
BfuAI ACCTGC 1 cut(s) 1193
BisI GCNGC 7 cut(s) 63, 510, 730, 869, 1654, 1981, 1984
BlpI GCTNAGC 1 cut(s) 1806
BlsI GCNGC 7 cut(s) 64, 511, 731, 870, 1655, 1982, 1985
Bme18I GGWCC 9 cut(s) 363, 382, 394, 1312, 1349, 1574, 1636, 1734, 1927
BmeRI GACNNNNNGTC 1 cut(s) 1572
BmeT110I CYCGRG 2 cut(s) 690, 1648
BmgBI CACGTC 1 cut(s) 900
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 3 cut(s) 93, 360, 1834
BmuI ACTGGG 1 cut(s) 432
BpiI GAAGAC 5 cut(s) 82, 486, 789, 946, 1477
BplI GAGNNNNNCTC 6 cut(s) 518, 550, 541, 573, 1799, 1831
BpmI CTGGAG 2 cut(s) 257, 1541
Bpu10I CCTNAGC 1 cut(s) 1374
Bpu1102I GCTNAGC 1 cut(s) 1806
Bpu14I TTCGAA 1 cut(s) 1265
BsaAI YACGTR 6 cut(s) 466, 679, 964, 1025, 1246, 1675
BsaHI GRCGYC 5 cut(s) 324, 567, 620, 1329, 1530
BsaI GGTCTC 2 cut(s) 155, 881
BsaWI WCCGGW 5 cut(s) 1340, 1351, 1363, 1488, 1918
BsaXI ACNNNNNCTCC 6 cut(s) 194, 224, 467, 497, 1539, 1569
Bse118I RCCGGY 3 cut(s) 1269, 1340, 1918
Bse1I ACTGG 5 cut(s) 181, 438, 787, 1604, 1622
BseBI CCWGG 3 cut(s) 496, 1287, 1309
BseGI GGATG 3 cut(s) 1765, 1915, 1957
BseMII CTCAG 5 cut(s) 676, 1180, 1365, 1797, 2020
BseNI ACTGG 5 cut(s) 181, 438, 787, 1604, 1622
BseRI GAGGAG 4 cut(s) 202, 671, 792, 1004
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 4 cut(s) 496, 1665, 1967, 1995
BseYI CCCAGC 2 cut(s) 403, 1931
BsgI GTGCAG 1 cut(s) 1105
Bsh1236I CGCG 9 cut(s) 572, 587, 884, 1019, 1097, 1226, 1257, 1625, 1631
Bsh1285I CGRYCG 5 cut(s) 525, 1213, 1305, 1493, 1502
BshFI GGCC 8 cut(s) 75, 180, 201, 736, 932, 1945, 1963, 1975
BshNI GGYRCC 2 cut(s) 1074, 1343
BshTI ACCGGT 2 cut(s) 1340, 1918
BsiEI CGRYCG 5 cut(s) 525, 1213, 1305, 1493, 1502
BsiHKAI GWGCWC 2 cut(s) 663, 1167
BsiHKCI CYCGRG 2 cut(s) 690, 1648
BsiWI CGTACG 1 cut(s) 1839
BslFI GGGAC 6 cut(s) 380, 656, 707, 712, 1325, 1913
BsmAI GTCTC 4 cut(s) 155, 881, 1538, 1555
BsmFI GGGAC 6 cut(s) 380, 656, 707, 712, 1325, 1913
BsnI GGCC 8 cut(s) 75, 180, 201, 736, 932, 1945, 1963, 1975
Bso31I GGTCTC 2 cut(s) 155, 881
BsoBI CYCGRG 2 cut(s) 690, 1648
Bsp119I TTCGAA 1 cut(s) 1265
Bsp1286I GDGCHC 5 cut(s) 495, 663, 1167, 1557, 1812
Bsp143I GATC 7 cut(s) 141, 245, 276, 1479, 1620, 1677, 1710
Bsp1720I GCTNAGC 1 cut(s) 1806
Bsp19I CCATGG 1 cut(s) 1577
Bsp68I TCGCGA 1 cut(s) 1226
BspANI GGCC 8 cut(s) 75, 180, 201, 736, 932, 1945, 1963, 1975
BspCNI CTCAG 5 cut(s) 675, 1179, 1366, 1798, 2019
BspFNI CGCG 9 cut(s) 572, 587, 884, 1019, 1097, 1226, 1257, 1625, 1631
BspMAI CTGCAG 1 cut(s) 1985
BspMI ACCTGC 1 cut(s) 1193
BspPI GGATC 4 cut(s) 240, 1487, 1628, 1672
BspQI GCTCTTC 1 cut(s) 1760
BspT104I TTCGAA 1 cut(s) 1265
BspT107I GGYRCC 2 cut(s) 1074, 1343
BspTI CTTAAG 1 cut(s) 2062
BspTNI GGTCTC 2 cut(s) 155, 881
BsrFI RCCGGY 3 cut(s) 1269, 1340, 1918
BsrI ACTGG 5 cut(s) 181, 438, 787, 1604, 1622
BssAI RCCGGY 3 cut(s) 1269, 1340, 1918
BssMI GATC 7 cut(s) 141, 245, 276, 1479, 1620, 1677, 1710
BssNAI GTATAC 1 cut(s) 463
BssNI GRCGYC 5 cut(s) 324, 567, 620, 1329, 1530
BssSI CACGAG 1 cut(s) 949
BssT1I CCWWGG 2 cut(s) 1577, 2054
Bst1107I GTATAC 1 cut(s) 463
Bst2BI CACGAG 1 cut(s) 949
Bst2UI CCWGG 3 cut(s) 496, 1287, 1309
Bst4CI ACNGT 6 cut(s) 1178, 1214, 1262, 1613, 1844, 1858
Bst6I CTCTTC 2 cut(s) 769, 1760
BstACI GRCGYC 5 cut(s) 324, 567, 620, 1329, 1530
BstAFI CTTAAG 1 cut(s) 2062
BstBAI YACGTR 6 cut(s) 466, 679, 964, 1025, 1246, 1675
BstBI TTCGAA 1 cut(s) 1265
BstC8I GCNNGC 3 cut(s) 491, 734, 2069
BstDEI CTNAG 6 cut(s) 47, 662, 1166, 1374, 1806, 2006
BstDSI CCRYGG 4 cut(s) 585, 927, 1577, 1745
BstF5I GGATG 3 cut(s) 1765, 1915, 1957
BstFNI CGCG 9 cut(s) 572, 587, 884, 1019, 1097, 1226, 1257, 1625, 1631
BstH2I RGCGCY 1 cut(s) 1374
BstHHI GCGC 2 cut(s) 1021, 1373
BstKTI GATC 7 cut(s) 144, 248, 279, 1482, 1623, 1680, 1713
BstMAI GTCTC 4 cut(s) 155, 881, 1538, 1555
BstMBI GATC 7 cut(s) 141, 245, 276, 1479, 1620, 1677, 1710
BstMCI CGRYCG 5 cut(s) 525, 1213, 1305, 1493, 1502
BstMWI GCNNNNNNNGC 6 cut(s) 37, 81, 865, 1009, 1018, 1659
BstNI CCWGG 3 cut(s) 496, 1287, 1309
BstSFI CTRYAG 1 cut(s) 1981
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 9 cut(s) 572, 587, 884, 1019, 1097, 1226, 1257, 1625, 1631
BstV1I GCAGC 4 cut(s) 496, 1665, 1967, 1995
BstV2I GAAGAC 5 cut(s) 82, 486, 789, 946, 1477
BstZ17I GTATAC 1 cut(s) 463
BsuRI GGCC 8 cut(s) 75, 180, 201, 736, 932, 1945, 1963, 1975
BtgI CCRYGG 4 cut(s) 585, 927, 1577, 1745
BtgZI GCGATG 2 cut(s) 907, 1446
BtrI CACGTC 1 cut(s) 900
BtsCI GGATG 3 cut(s) 1765, 1915, 1957
BtsI GCAGTG 1 cut(s) 1999
BtsIMutI CAGTG 3 cut(s) 794, 1999, 2014
BtuMI TCGCGA 1 cut(s) 1226
BveI ACCTGC 1 cut(s) 1193
Cac8I GCNNGC 3 cut(s) 491, 734, 2069
CaiI CAGNNNCTG 1 cut(s) 1564
CfoI GCGC 2 cut(s) 1021, 1373
Cfr10I RCCGGY 3 cut(s) 1269, 1340, 1918
Cfr42I CCGCGG 1 cut(s) 588
Cfr9I CCCGGG 1 cut(s) 1648
CpoI CGGWCCG 2 cut(s) 1349, 1636
CseI GACGC 6 cut(s) 332, 556, 609, 1318, 1538, 1620
Csp6I GTAC 6 cut(s) 666, 1026, 1075, 1179, 1672, 1840
CspAI ACCGGT 2 cut(s) 1340, 1918
CspCI CAANNNNNGTGG 2 cut(s) 834, 869
CspI CGGWCCG 2 cut(s) 1349, 1636
CviAII CATG 4 cut(s) 71, 997, 1525, 1578
CviQI GTAC 6 cut(s) 666, 1026, 1075, 1179, 1672, 1840
DdeI CTNAG 6 cut(s) 47, 662, 1166, 1374, 1806, 2006
DpnI GATC 7 cut(s) 143, 247, 278, 1481, 1622, 1679, 1712
DpnII GATC 7 cut(s) 141, 245, 276, 1479, 1620, 1677, 1710
DraIII CACNNNGTG 1 cut(s) 1593
DrdI GACNNNNNNGTC 1 cut(s) 650
DriI GACNNNNNGTC 1 cut(s) 1572
DseDI GACNNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 4 cut(s) 178, 199, 734, 930
Eam1104I CTCTTC 2 cut(s) 769, 1760
Eam1105I GACNNNNNGTC 1 cut(s) 1572
EarI CTCTTC 2 cut(s) 769, 1760
EciI GGCGGA 2 cut(s) 355, 947
Ecl136II GAGCTC 1 cut(s) 661
Eco130I CCWWGG 2 cut(s) 1577, 2054
Eco147I AGGCCT 1 cut(s) 1975
Eco24I GRGCYC 3 cut(s) 663, 1557, 1812
Eco31I GGTCTC 2 cut(s) 155, 881
Eco32I GATATC 1 cut(s) 1384
Eco47I GGWCC 9 cut(s) 363, 382, 394, 1312, 1349, 1574, 1636, 1734, 1927
Eco47III AGCGCT 1 cut(s) 1372
Eco53kI GAGCTC 1 cut(s) 661
Eco57I CTGAAG 2 cut(s) 562, 1112
Eco72I CACGTG 1 cut(s) 964
Eco88I CYCGRG 2 cut(s) 690, 1648
EcoICRI GAGCTC 1 cut(s) 661
EcoO109I RGGNCCY 3 cut(s) 394, 1312, 1927
EcoRI GAATTC 1 cut(s) 1897
EcoRII CCWGG 3 cut(s) 494, 1285, 1307
EcoRV GATATC 1 cut(s) 1384
EcoT14I CCWWGG 2 cut(s) 1577, 2054
EcoT38I GRGCYC 3 cut(s) 663, 1557, 1812
ErhI CCWWGG 2 cut(s) 1577, 2054
FaeI CATG 4 cut(s) 74, 1000, 1528, 1581
FaqI GGGAC 6 cut(s) 380, 656, 707, 712, 1325, 1913
FatI CATG 4 cut(s) 70, 996, 1524, 1577
FauI CCCGC 5 cut(s) 592, 901, 1649, 1712, 1830
FblI GTMKAC 2 cut(s) 462, 1281
Fnu4HI GCNGC 7 cut(s) 63, 510, 730, 869, 1654, 1981, 1984
FokI GGATG 3 cut(s) 1772, 1922, 1964
FriOI GRGCYC 3 cut(s) 663, 1557, 1812
Fsp4HI GCNGC 7 cut(s) 63, 510, 730, 869, 1654, 1981, 1984
FspBI CTAG 2 cut(s) 1977, 2019
GlaI GCGC 2 cut(s) 1020, 1372
GluI GCNGC 7 cut(s) 63, 510, 730, 869, 1654, 1981, 1984
GsaI CCCAGC 2 cut(s) 407, 1935
GsuI CTGGAG 2 cut(s) 257, 1541
HaeII RGCGCY 1 cut(s) 1374
HaeIII GGCC 8 cut(s) 75, 180, 201, 736, 932, 1945, 1963, 1975
HgaI GACGC 6 cut(s) 332, 556, 609, 1318, 1538, 1620
HhaI GCGC 2 cut(s) 1021, 1373
Hin1I GRCGYC 5 cut(s) 324, 567, 620, 1329, 1530
Hin1II CATG 4 cut(s) 74, 1000, 1528, 1581
Hin6I GCGC 2 cut(s) 1019, 1371
HinP1I GCGC 2 cut(s) 1019, 1371
HincII GTYRAC 6 cut(s) 190, 748, 756, 1402, 1425, 1890
HindII GTYRAC 6 cut(s) 190, 748, 756, 1402, 1425, 1890
HindIII AAGCTT 2 cut(s) 1118, 1293
HpaI GTTAAC 1 cut(s) 1425
Hpy188I TCNGA 9 cut(s) 245, 1131, 1152, 1169, 1636, 1786, 1876, 1951, 2031
HpyAV CCTTC 8 cut(s) 87, 629, 979, 1197, 1231, 1459, 1464, 1532
HpyCH4III ACNGT 6 cut(s) 1178, 1214, 1262, 1613, 1844, 1858
HpyCH4V TGCA 5 cut(s) 1086, 1361, 1983, 1999, 2084
HpyF10VI GCNNNNNNNGC 6 cut(s) 37, 81, 865, 1009, 1018, 1659
HpyF3I CTNAG 6 cut(s) 47, 662, 1166, 1374, 1806, 2006
Hsp92I GRCGYC 5 cut(s) 324, 567, 620, 1329, 1530
Hsp92II CATG 4 cut(s) 74, 1000, 1528, 1581
HspAI GCGC 2 cut(s) 1019, 1371
KflI GGGWCCC 2 cut(s) 1312, 1927
KpnI GGTACC 1 cut(s) 1078
KspAI GTTAAC 1 cut(s) 1425
KspI CCGCGG 1 cut(s) 588
Kzo9I GATC 7 cut(s) 141, 245, 276, 1479, 1620, 1677, 1710
LguI GCTCTTC 1 cut(s) 1760
LmnI GCTCC 9 cut(s) 36, 658, 827, 856, 967, 1000, 1017, 1560, 1659
Lsp1109I GCAGC 4 cut(s) 496, 1665, 1967, 1995
LweI GCATC 3 cut(s) 93, 360, 1834
MaeI CTAG 2 cut(s) 1977, 2019
MaeIII GTNAC 8 cut(s) 146, 286, 862, 900, 1205, 1595, 1613, 1879
MalI GATC 7 cut(s) 143, 247, 278, 1481, 1622, 1679, 1712
MboI GATC 7 cut(s) 141, 245, 276, 1479, 1620, 1677, 1710
MhlI GDGCHC 5 cut(s) 495, 663, 1167, 1557, 1812
MlsI TGGCCA 2 cut(s) 180, 932
MluCI AATT 6 cut(s) 10, 15, 1146, 1355, 1862, 1897
MluI ACGCGT 1 cut(s) 882
MluNI TGGCCA 2 cut(s) 180, 932
MlyI GAGTC 9 cut(s) 476, 550, 682, 694, 717, 761, 851, 1000, 1072
MmeI TCCRAC 4 cut(s) 333, 612, 1614, 1929
Mox20I TGGCCA 2 cut(s) 180, 932
MscI TGGCCA 2 cut(s) 180, 932
MseI TTAA 2 cut(s) 1424, 2063
MslI CAYNNNNRTG 3 cut(s) 677, 926, 1921
Msp20I TGGCCA 2 cut(s) 180, 932
MspA1I CMGCKG 3 cut(s) 587, 1656, 1805
MspCI CTTAAG 1 cut(s) 2062
MvaI CCWGG 3 cut(s) 496, 1287, 1309
MvnI CGCG 9 cut(s) 572, 587, 884, 1019, 1097, 1226, 1257, 1625, 1631
MwoI GCNNNNNNNGC 6 cut(s) 37, 81, 865, 1009, 1018, 1659
NcoI CCATGG 1 cut(s) 1577
NdeII GATC 7 cut(s) 141, 245, 276, 1479, 1620, 1677, 1710
NlaIII CATG 4 cut(s) 74, 1000, 1528, 1581
NmeAIII GCCGAG 5 cut(s) 515, 731, 762, 988, 1558
NmuCI GTSAC 5 cut(s) 146, 286, 900, 1205, 1613
NruI TCGCGA 1 cut(s) 1226
NspV TTCGAA 1 cut(s) 1265
OliI CACNNNNGTG 1 cut(s) 1921
PaqCI CACCTGC 1 cut(s) 1193
PceI AGGCCT 1 cut(s) 1975
PciSI GCTCTTC 1 cut(s) 1760
PcsI WCGNNNNNNNCGW 3 cut(s) 617, 651, 1671
PfeI GAWTC 6 cut(s) 168, 240, 338, 1058, 1170, 1407
Pfl23II CGTACG 1 cut(s) 1839
PflMI CCANNNNNTGG 1 cut(s) 40
PinAI ACCGGT 2 cut(s) 1340, 1918
PkrI GCNGC 7 cut(s) 64, 511, 731, 870, 1655, 1982, 1985
PleI GAGTC 9 cut(s) 476, 550, 682, 694, 716, 760, 850, 1000, 1071
PmaCI CACGTG 1 cut(s) 964
PmlI CACGTG 1 cut(s) 964
PpsI GAGTC 9 cut(s) 476, 550, 682, 694, 716, 760, 850, 1000, 1071
Ppu21I YACGTR 6 cut(s) 466, 679, 964, 1025, 1246, 1675
PpuMI RGGWCCY 3 cut(s) 394, 1312, 1927
Psp124BI GAGCTC 1 cut(s) 663
Psp5II RGGWCCY 3 cut(s) 394, 1312, 1927
Psp6I CCWGG 3 cut(s) 494, 1285, 1307
PspCI CACGTG 1 cut(s) 964
PspFI CCCAGC 2 cut(s) 403, 1931
PspGI CCWGG 3 cut(s) 494, 1285, 1307
PspLI CGTACG 1 cut(s) 1839
PspPPI RGGWCCY 3 cut(s) 394, 1312, 1927
PstI CTGCAG 1 cut(s) 1985
PstNI CAGNNNCTG 1 cut(s) 1564
PvuII CAGCTG 1 cut(s) 1805
RruI TCGCGA 1 cut(s) 1226
RsaI GTAC 6 cut(s) 667, 1027, 1076, 1180, 1673, 1841
RsaNI GTAC 6 cut(s) 666, 1026, 1075, 1179, 1672, 1840
RseI CAYNNNNRTG 3 cut(s) 677, 926, 1921
Rsr2I CGGWCCG 2 cut(s) 1349, 1636
RsrII CGGWCCG 2 cut(s) 1349, 1636
SacI GAGCTC 1 cut(s) 663
SacII CCGCGG 1 cut(s) 588
SapI GCTCTTC 1 cut(s) 1760
SaqAI TTAA 2 cut(s) 1424, 2063
SatI GCNGC 7 cut(s) 63, 510, 730, 869, 1654, 1981, 1984
Sau3AI GATC 7 cut(s) 141, 245, 276, 1479, 1620, 1677, 1710
SchI GAGTC 9 cut(s) 476, 550, 682, 694, 717, 761, 851, 1000, 1072
SduI GDGCHC 5 cut(s) 495, 663, 1167, 1557, 1812
SfaNI GCATC 3 cut(s) 93, 360, 1834
SfcI CTRYAG 1 cut(s) 1981
Sfr303I CCGCGG 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 1265
SgrAI CRCCGGYG 2 cut(s) 1340, 1918
SgrBI CCGCGG 1 cut(s) 588
SinI GGWCC 9 cut(s) 363, 382, 394, 1312, 1349, 1574, 1636, 1734, 1927
SmaI CCCGGG 1 cut(s) 1650
SmiMI CAYNNNNRTG 3 cut(s) 677, 926, 1921
SmlI CTYRAG 1 cut(s) 2062
SmoI CTYRAG 1 cut(s) 2062
Sse9I AATT 6 cut(s) 10, 15, 1146, 1355, 1862, 1897
SseBI AGGCCT 1 cut(s) 1975
SspMI CTAG 2 cut(s) 1977, 2019
SstI GAGCTC 1 cut(s) 663
StuI AGGCCT 1 cut(s) 1975
StyI CCWWGG 2 cut(s) 1577, 2054
TaaI ACNGT 6 cut(s) 1178, 1214, 1262, 1613, 1844, 1858
TaqII GACCGA 3 cut(s) 539, 1419, 1488
TasI AATT 6 cut(s) 10, 15, 1146, 1355, 1862, 1897
TatI WGTACW 1 cut(s) 665
TauI GCSGC 3 cut(s) 65, 732, 871
TfiI GAWTC 6 cut(s) 168, 240, 338, 1058, 1170, 1407
Tru1I TTAA 2 cut(s) 1424, 2063
Tru9I TTAA 2 cut(s) 1424, 2063
TscAI CASTG 3 cut(s) 794, 2006, 2014
TseFI GTSAC 5 cut(s) 146, 286, 900, 1205, 1613
TseI GCWGC 4 cut(s) 509, 1653, 1980, 1983
Tsp45I GTSAC 5 cut(s) 146, 286, 900, 1205, 1613
TspDTI ATGAA 6 cut(s) 17, 440, 910, 985, 1036, 1927
TspGWI ACGGA 9 cut(s) 175, 333, 1150, 1485, 1492, 1583, 1598, 1704, 1747
TspMI CCCGGG 1 cut(s) 1648
TspRI CASTG 3 cut(s) 794, 2006, 2014
Van91I CCANNNNNTGG 1 cut(s) 40
Vha464I CTTAAG 1 cut(s) 2062
VpaK11BI GGWCC 9 cut(s) 363, 382, 394, 1312, 1349, 1574, 1636, 1734, 1927
XapI RAATTY 2 cut(s) 1355, 1897
XmaI CCCGGG 1 cut(s) 1648
XmiI GTMKAC 2 cut(s) 462, 1281
XspI CTAG 2 cut(s) 1977, 2019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.