FvH4_2g29180

SPX domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012916
Physical Location & Seq
Forward (+)
22693023 .. 22695809
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29180.t1

Sequence Viewer

Length: 864 bp
ATGAAGTTCTCGAAGAGCCTCGGCATCCTGGTCGAGGAGGCCTTGCCGGAATGGCGGGACAAGTTCTTGTCCTACAAGGACCTCAAGAAGCAATTGAAGCTGATCTATCCCAAAGACGGCGACAAGCCGCCGAGTAAACGGCCGAGATTGACCGACGACGAGGCGCGCACGGACGACGAGGGCGGTGACGTGTCCAAGGAGGTCGCTGATTTCGTGAGGTTGTTGGAGAATGAAATGGAGAAGTTCAATTCGTTTTTTGAGGAGAAAGAAGAAGAGTACGTGATTAGATGGAAGGAGCTACAGGATAGGGTGGCAGAAGCCAAAGATTCAAATGAGGAGTTGATGAGAGTAGGGAGAGAAATAGTTGATTTTCATGGAGAGATGGTTTTGTTAGAGAACTACAGTGCACTCAACTATACAGGACTATTGAAGATTCTGAAGAAACATGACAAGCGAACTGGTGTTCTCATTCGGTTTCCCTTTGTCCAACGGGTCATGCAACAGCCATTCTTCAGTACTGAGGTACTCGATAAGCTCGTGAAGGAGTGTGAGGGGATGCTTGATCACGTCTTCTCCAGGAATGACCCATCAGGTCCATCTGAAGCAGCCAAAGCAGAAGAACAGAGCGACTCTGAGCCTGTGACTGAAACTAGAGAAATTGTACGGAAAGCTCCTATAGAACTTGCAGAAATAAAGCAATTGGAGAGCGTGTACGGGAAGCAAACTGAAACCGCATTGCGTGTCTTGAAGGATATTCGGAGTGGAAGCTCAACTGTGAGTGCATTTTCATTGCCCCCTTTGCAAATAAATGTGGTGGAGGAGGATTCAAAGAACATACATGTTTTACAACAAGAAGCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPX

Protein Analysis

288

Amino Acids

33.07

Weight (kDa)

5.1

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 1 - 50 1.1e-07 SPX domain
SPX PF03105 119 - 161 1.7e-07 SPX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 166
AciI CCGC 4 cut(s) 55, 128, 183, 732
AcoI YGGCCR 1 cut(s) 140
AcuI CTGAAG 3 cut(s) 458, 496, 621
AfaI GTAC 5 cut(s) 278, 517, 525, 663, 713
AfiI CCNNNNNNNGG 2 cut(s) 34, 116
AflIII ACRYGT 2 cut(s) 189, 838
AgsI TTSAA 6 cut(s) 97, 247, 330, 430, 748, 828
AjiI CACGTC 2 cut(s) 190, 568
AjnI CCWGG 2 cut(s) 27, 575
AluBI AGCT 5 cut(s) 100, 298, 535, 671, 768
AluI AGCT 5 cut(s) 100, 298, 535, 671, 768
Alw21I GWGCWC 1 cut(s) 409
Alw44I GTGCAC 1 cut(s) 405
AoxI GGCC 2 cut(s) 39, 140
ApaLI GTGCAC 1 cut(s) 405
ApeKI GCWGC 1 cut(s) 605
AspLEI GCGC 2 cut(s) 166, 168
AspS9I GGNCC 2 cut(s) 79, 593
AsuHPI GGTGA 1 cut(s) 197
AvaII GGWCC 2 cut(s) 79, 593
BaeGI GKGCMC 1 cut(s) 409
BauI CACGAG 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 617
BccI CCATC 4 cut(s) 282, 376, 595, 604
BceAI ACGGC 2 cut(s) 133, 155
BcgI CGANNNNNNTGC 2 cut(s) 13, 47
BciT130I CCWGG 2 cut(s) 29, 577
BclI TGATCA 1 cut(s) 562
BfaI CTAG 1 cut(s) 651
BfmI CTRYAG 3 cut(s) 299, 400, 675
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 2 cut(s) 128, 606
BlsI GCNGC 2 cut(s) 129, 607
BmcAI AGTACT 1 cut(s) 517
Bme1390I CCNGG 2 cut(s) 29, 577
Bme18I GGWCC 2 cut(s) 79, 593
BmgBI CACGTC 2 cut(s) 190, 568
BmgT120I GGNCC 2 cut(s) 79, 593
BmrFI CCNGG 2 cut(s) 29, 577
BmsI GCATC 2 cut(s) 33, 546
BpiI GAAGAC 1 cut(s) 562
BpmI CTGGAG 1 cut(s) 559
BpuEI CTTGAG 1 cut(s) 68
BsaAI YACGTR 1 cut(s) 280
BsaJI CCNNGG 2 cut(s) 19, 195
BsaXI ACNNNNNCTCC 6 cut(s) 369, 399, 557, 587, 695, 725
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 116
Bse1I ACTGG 1 cut(s) 463
Bse3DI GCAATG 2 cut(s) 734, 788
BseBI CCWGG 2 cut(s) 29, 577
BseDI CCNNGG 2 cut(s) 19, 195
BseGI GGATG 2 cut(s) 24, 561
BseLI CCNNNNNNNGG 2 cut(s) 34, 116
BseMI GCAATG 2 cut(s) 734, 788
BseMII CTCAG 2 cut(s) 510, 624
BseNI ACTGG 1 cut(s) 463
BsePI GCGCGC 1 cut(s) 164
BseRI GAGGAG 4 cut(s) 50, 275, 350, 833
BseSI GKGCMC 1 cut(s) 409
BseX3I CGGCCG 1 cut(s) 140
BseXI GCAGC 1 cut(s) 617
Bsh1236I CGCG 1 cut(s) 166
Bsh1285I CGRYCG 1 cut(s) 143
BshFI GGCC 2 cut(s) 41, 142
BsiEI CGRYCG 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 409
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 2 cut(s) 34, 116
BsmFI GGGAC 1 cut(s) 71
BsnI GGCC 2 cut(s) 41, 142
Bsp1286I GDGCHC 1 cut(s) 409
Bsp143I GATC 2 cut(s) 102, 562
BspACI CCGC 4 cut(s) 55, 128, 183, 732
BspANI GGCC 2 cut(s) 41, 142
BspCNI CTCAG 2 cut(s) 511, 625
BspFNI CGCG 1 cut(s) 166
BspQI GCTCTTC 1 cut(s) 8
BsrDI GCAATG 2 cut(s) 734, 788
BsrI ACTGG 1 cut(s) 463
BssECI CCNNGG 2 cut(s) 19, 195
BssHII GCGCGC 1 cut(s) 164
BssMI GATC 2 cut(s) 102, 562
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 1 cut(s) 195
Bst2BI CACGAG 1 cut(s) 536
Bst2UI CCWGG 2 cut(s) 29, 577
Bst4CI ACNGT 2 cut(s) 404, 775
Bst6I CTCTTC 2 cut(s) 8, 267
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 2 cut(s) 519, 633
BstENI CCTNNNNNAGG 1 cut(s) 32
BstF5I GGATG 2 cut(s) 24, 561
BstFNI CGCG 1 cut(s) 166
BstHHI GCGC 2 cut(s) 166, 168
BstKTI GATC 2 cut(s) 105, 565
BstMBI GATC 2 cut(s) 102, 562
BstMCI CGRYCG 1 cut(s) 143
BstMWI GCNNNNNNNGC 4 cut(s) 52, 97, 611, 799
BstNI CCWGG 2 cut(s) 29, 577
BstNSI RCATGY 1 cut(s) 842
BstSCI CCNGG 2 cut(s) 27, 575
BstSFI CTRYAG 3 cut(s) 299, 400, 675
BstSLI GKGCMC 1 cut(s) 409
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 1 cut(s) 617
BstV2I GAAGAC 1 cut(s) 562
BstZI CGGCCG 1 cut(s) 140
BsuRI GGCC 2 cut(s) 41, 142
BtrI CACGTC 2 cut(s) 190, 568
BtsCI GGATG 2 cut(s) 24, 561
BtsIMutI CAGTG 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 166
CfoI GCGC 2 cut(s) 166, 168
Cfr13I GGNCC 2 cut(s) 79, 593
Csp6I GTAC 5 cut(s) 277, 516, 524, 662, 712
CviAII CATG 4 cut(s) 374, 446, 496, 839
CviQI GTAC 5 cut(s) 277, 516, 524, 662, 712
DdeI CTNAG 2 cut(s) 519, 633
DpnI GATC 2 cut(s) 104, 564
DpnII GATC 2 cut(s) 102, 562
EaeI YGGCCR 1 cut(s) 140
EagI CGGCCG 1 cut(s) 140
Eam1104I CTCTTC 2 cut(s) 8, 267
EarI CTCTTC 2 cut(s) 8, 267
EclXI CGGCCG 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 195
Eco147I AGGCCT 1 cut(s) 41
Eco47I GGWCC 2 cut(s) 79, 593
Eco52I CGGCCG 1 cut(s) 140
Eco57I CTGAAG 3 cut(s) 458, 496, 621
EcoNI CCTNNNNNAGG 1 cut(s) 32
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 2 cut(s) 27, 575
EcoT14I CCWWGG 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 4 cut(s) 377, 449, 499, 842
FaiI YATR 7 cut(s) 375, 417, 447, 497, 677, 836, 840
FaqI GGGAC 1 cut(s) 71
FatI CATG 4 cut(s) 373, 445, 495, 838
FauI CCCGC 1 cut(s) 48
FbaI TGATCA 1 cut(s) 562
Fnu4HI GCNGC 2 cut(s) 128, 606
FokI GGATG 2 cut(s) 11, 568
Fsp4HI GCNGC 2 cut(s) 128, 606
FspBI CTAG 1 cut(s) 651
GlaI GCGC 2 cut(s) 165, 167
GluI GCNGC 2 cut(s) 128, 606
GsuI CTGGAG 1 cut(s) 559
HaeIII GGCC 2 cut(s) 41, 142
HapII CCGG 1 cut(s) 47
HhaI GCGC 2 cut(s) 166, 168
Hin1II CATG 4 cut(s) 377, 449, 499, 842
Hin6I GCGC 2 cut(s) 164, 166
HinP1I GCGC 2 cut(s) 164, 166
HinfI GANTC 4 cut(s) 326, 433, 629, 824
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 3 cut(s) 137, 407, 712
Hpy188I TCNGA 4 cut(s) 438, 601, 634, 759
Hpy188III TCNNGA 5 cut(s) 10, 85, 214, 538, 745
Hpy8I GTNNAC 3 cut(s) 137, 407, 712
Hpy99I CGWCG 3 cut(s) 158, 161, 179
HpyAV CCTTC 3 cut(s) 286, 535, 742
HpyCH4III ACNGT 2 cut(s) 404, 775
HpyCH4IV ACGT 3 cut(s) 189, 279, 567
HpyCH4V TGCA 5 cut(s) 407, 499, 686, 782, 802
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 97, 611, 799
HpyF3I CTNAG 2 cut(s) 519, 633
HpySE526I ACGT 3 cut(s) 189, 279, 567
Hsp92II CATG 4 cut(s) 377, 449, 499, 842
HspAI GCGC 2 cut(s) 164, 166
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 2 cut(s) 102, 562
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 2 cut(s) 295, 676
Lsp1109I GCAGC 1 cut(s) 617
LweI GCATC 2 cut(s) 33, 546
MaeI CTAG 1 cut(s) 651
MaeII ACGT 3 cut(s) 189, 279, 567
MaeIII GTNAC 2 cut(s) 185, 640
MalI GATC 2 cut(s) 104, 564
MboI GATC 2 cut(s) 102, 562
MboII GAAGA 8 cut(s) 25, 281, 284, 442, 451, 502, 562, 629
MfeI CAATTG 2 cut(s) 92, 698
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 4 cut(s) 92, 247, 657, 698
MlyI GAGTC 1 cut(s) 623
MmeI TCCRAC 2 cut(s) 204, 511
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 29, 577
MunI CAATTG 2 cut(s) 92, 698
MvaI CCWGG 2 cut(s) 29, 577
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 4 cut(s) 52, 97, 611, 799
NdeII GATC 2 cut(s) 102, 562
NlaIII CATG 4 cut(s) 377, 449, 499, 842
NmeAIII GCCGAG 2 cut(s) 156, 168
NmuCI GTSAC 2 cut(s) 185, 640
NspI RCATGY 1 cut(s) 842
PauI GCGCGC 1 cut(s) 164
PceI AGGCCT 1 cut(s) 41
PciI ACATGT 1 cut(s) 838
PciSI GCTCTTC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 534
PfeI GAWTC 3 cut(s) 326, 433, 824
PfoI TCCNGGA 1 cut(s) 575
PkrI GCNGC 2 cut(s) 129, 607
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
Ppu21I YACGTR 1 cut(s) 280
PpuMI RGGWCCY 1 cut(s) 79
PscI ACATGT 1 cut(s) 838
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 27, 575
PspGI CCWGG 2 cut(s) 27, 575
PspPI GGNCC 2 cut(s) 79, 593
PspPPI RGGWCCY 1 cut(s) 79
PteI GCGCGC 1 cut(s) 164
RsaI GTAC 5 cut(s) 278, 517, 525, 663, 713
RsaNI GTAC 5 cut(s) 277, 516, 524, 662, 712
SapI GCTCTTC 1 cut(s) 8
SatI GCNGC 2 cut(s) 128, 606
Sau3AI GATC 2 cut(s) 102, 562
Sau96I GGNCC 2 cut(s) 79, 593
ScaI AGTACT 1 cut(s) 517
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 29, 577
SduI GDGCHC 1 cut(s) 409
SfaNI GCATC 2 cut(s) 33, 546
SfcI CTRYAG 3 cut(s) 299, 400, 675
SinI GGWCC 2 cut(s) 79, 593
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 4 cut(s) 92, 247, 657, 698
SseBI AGGCCT 1 cut(s) 41
SsiI CCGC 4 cut(s) 55, 128, 183, 732
SspMI CTAG 1 cut(s) 651
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 27, 575
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 2 cut(s) 404, 775
TaiI ACGT 3 cut(s) 192, 282, 570
TaqI TCGA 3 cut(s) 11, 33, 528
TaqII GACCGA 1 cut(s) 167
TasI AATT 4 cut(s) 92, 247, 657, 698
TatI WGTACW 1 cut(s) 515
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 3 cut(s) 326, 433, 824
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 2 cut(s) 185, 640
TseI GCWGC 1 cut(s) 605
Tsp45I GTSAC 2 cut(s) 185, 640
TspDTI ATGAA 4 cut(s) 17, 246, 362, 777
TspGWI ACGGA 2 cut(s) 185, 679
TspRI CASTG 1 cut(s) 409
VneI GTGCAC 1 cut(s) 405
VpaK11BI GGWCC 2 cut(s) 79, 593
XagI CCTNNNNNAGG 1 cut(s) 32
XceI RCATGY 1 cut(s) 842
XspI CTAG 1 cut(s) 651
ZrmI AGTACT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.