Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004709

Physical Location & Seq

Genomic Coordinates Reverse (-)

24240798 .. 24243965

Gene Structure 3168 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g31680.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGGGGATGGAGTTTTTGATTACCAATGGTTCTCTGGTGTCAACCATTTTCACAGTGCTGGCAGTATTGGCTGGAGTCTTGGGATACTTGTATGCACCATACTGGGGAGTGAGAAGGGTTCCTGGCCCACGAACTATCCCTTTTCTAGGACACCTTCCCTTGCTGGCTAAGCATGGTCCTGATTTGTTCTCAGTTCTTGCCAAGCAATATGGCCCTATCTTCAGGTTTCATATGGGAAGGCAGCCACTTATAATTGTAGCAGATGCAGAACTTTGTAGAGAAGTTGGAATCAAGAAATTCAAAGACATTCCAAACAGAAGCATACCATCTCCAATCTCAGCCTCACCTCTTCACCAAAAGGGTCTCTTTTTCACCAGGGATATAAGATGGTCAACCATGAGAAACACCATTTTATCAGTGTATCAACCTTCATACCTAGCCAGCCTTGTTCCCACCATGCAATCATATATTGAATCTGCAACTCAAAACCTTGATGACTCTTCCAACAAGGAAGACATTACCTTTTCAAACCTCTCACTCAGATTGGCCACTGATGTTATTGGACAAGCTTCCTTTGGTGTCGACTTTGGCCTTTCAAAACCACAGTCTATTAGTAATTCAATCAACAAGGTTGATAATGGTGAAGACAAGAATGATGATGAAGTTTCAGATTTTATCAACCAACACATCTATTCCACAACTCAGCTTAAGATGGACTTATCAGGTTCCTTATCAATCATATTGGGACTACTTGTCCCCATAATACAAGAGCCATTTAGGCAAATCTTGAAGAGAATTCCGGGCACTATGGACTGGAAAGTAGACCGGACTAACCAGAATTTGGCAGGCCGGCTTGATCAGATTGTAATGAGGAGAATGAAAGATAGCGATCGAGGTACCAAGAATTTCTTGTCACTCATAATGAATGCAAGGGAGTCAGAGGGGGTAGCAAAGAGTGTGTTCACTCCAGACTACATTAGTGCAGTCACTTATGAACATTTGCTTGCTGGGTCGGCCACCACATCTTTTACATTGTCCTCAGCTGTTTACTTGATTTCTGGACACCCGGAAGTGGAAAGCAAGTTGCTAGCAGAAATCGATGGATTTGGTCCACATGATCAGATCCCAACAGCTCCTGATCTTCAGCACAAATTCCCTTATCTTGAACAGGTAAGTAGCATGAAACAAGAGCTAATCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0006629 GO:0006720 GO:0006721 GO:0006810 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009914 GO:0009926 GO:0009934 GO:0009962 GO:0009963 GO:0009987 GO:0010016 GO:0010223 GO:0010346 GO:0010817 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0019222 GO:0022603 GO:0032501 GO:0032502 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0046148 GO:0048367 GO:0048509 GO:0048518 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:1901576 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

p450

Protein Analysis

401

Amino Acids

44.48

Weight (kDa)

6.76

Isoelectric Point (pI)

32.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	41 - 522	3.6e-84	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004709)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26170 AT2G26170
fragaria_vesca	FvH4_2g31660 FvH4_2g31680 FvH4_2g31680 FvH4_2g31680 FvH4_2g31680 FvH4_2g31680
malus_domestica	MD02G1142400.v1.1 MD08G1064200.v1.1 MD15G1057500.v1.1 MD15G1057600.v1.1
prunus_persica	Prupe.1G410100_v2.0.a1 Prupe.1G410100_v2.0.a1 Prupe.1G410300_v2.0.a1 Prupe.1G410300_v2.0.a1 Prupe.1G410300_v2.0.a1
pyrus_communis	pycom08g05270 pycom15g05410 pycom15g05420 pycom15g22400
rosa_chinensis	RchiOBHm_Chr6g0311161
rosa_laevigata	RLG00000010431
rosa_multiflora	Rmu_sc0008967.1_g000014 Rmu_sc0016962.1_g000003
rosa_roxburghii	Rroxscaffold_7G00157950
rosa_rugosa	Rorug06G0386000
rosa_samantha	Rh6BG510700 Rh6CG516400 Rh6DG502900
rosa_wichuraiana	Rw6G043550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	251
Acc65I	GGTACC	1 cut(s)	896
AccB1I	GGYRCC	1 cut(s)	896
AccB7I	CCANNNNNTGG	1 cut(s)	841
AccI	GTMKAC	2 cut(s)	582, 822
AclWI	GGATC	1 cut(s)	1117
AcoI	YGGCCR	2 cut(s)	546, 1014
AcsI	RAATTY	5 cut(s)	296, 795, 838, 904, 1151
AcuI	CTGAAG	2 cut(s)	205, 1127
AfaI	GTAC	1 cut(s)	898
AfiI	CCNNNNNNNGG	4 cut(s)	104, 146, 841, 1072
AflII	CTTAAG	1 cut(s)	707
AgsI	TTSAA	7 cut(s)	301, 473, 528, 597, 621, 790, 1166
AhdI	GACNNNNNGTC	1 cut(s)	752
AjnI	CCWGG	2 cut(s)	121, 374
AluBI	AGCT	5 cut(s)	569, 706, 1043, 1133, 1192
AluI	AGCT	5 cut(s)	569, 706, 1043, 1133, 1192
Alw26I	GTCTC	1 cut(s)	368
AlwI	GGATC	1 cut(s)	1117
AlwNI	CAGNNNCTG	1 cut(s)	1136
AoxI	GGCC	6 cut(s)	124, 211, 546, 589, 847, 1014
ApeKI	GCWGC	1 cut(s)	241
ApoI	RAATTY	5 cut(s)	296, 795, 838, 904, 1151
ArsI	GACNNNNNNTTYG	2 cut(s)	590, 622
Asp718I	GGTACC	1 cut(s)	896
AspS9I	GGNCC	4 cut(s)	125, 176, 212, 1109
AsuC2I	CCSGG	2 cut(s)	801, 1067
AsuHPI	GGTGA	4 cut(s)	336, 344, 364, 653
AsuNHI	GCTAGC	1 cut(s)	1087
AvaII	GGWCC	2 cut(s)	176, 1109
BaeGI	GKGCMC	1 cut(s)	806
BalI	TGGCCA	1 cut(s)	548
BanI	GGYRCC	1 cut(s)	896
BbsI	GAAGAC	2 cut(s)	519, 651
BbvCI	CCTCAGC	1 cut(s)	1039
BbvI	GCAGC	1 cut(s)	253
BccI	CCATC	4 cut(s)	334, 381, 706, 1094
BciT130I	CCWGG	2 cut(s)	123, 376
BciVI	GTATCC	1 cut(s)	77
BclI	TGATCA	2 cut(s)	856, 1117
BcnI	CCSGG	2 cut(s)	801, 1067
BcoDI	GTCTC	1 cut(s)	368
BfaI	CTAG	3 cut(s)	146, 437, 1088
BfrI	CTTAAG	1 cut(s)	707
BfuI	GTATCC	1 cut(s)	77
BglI	GCCNNNNNGGC	1 cut(s)	778
BisI	GCNGC	1 cut(s)	242
BlpI	GCTNAGC	1 cut(s)	168
BlsI	GCNGC	1 cut(s)	243
Bme1390I	CCNGG	4 cut(s)	123, 376, 801, 1067
Bme18I	GGWCC	2 cut(s)	176, 1109
BmeRI	GACNNNNNGTC	1 cut(s)	752
BmgT120I	GGNCC	4 cut(s)	125, 176, 212, 1109
BmiI	GGNNCC	3 cut(s)	120, 727, 898
BmrFI	CCNGG	4 cut(s)	123, 376, 801, 1067
BmrI	ACTGGG	1 cut(s)	112
BmsI	GCATC	1 cut(s)	253
BmtI	GCTAGC	1 cut(s)	1091
BmuI	ACTGGG	1 cut(s)	112
BpiI	GAAGAC	2 cut(s)	519, 651
BpmI	CTGGAG	2 cut(s)	93, 951
Bpu10I	CCTNAGC	1 cut(s)	1039
Bpu1102I	GCTNAGC	1 cut(s)	168
BpuMI	CCSGG	2 cut(s)	801, 1067
Bsa29I	ATCGAT	1 cut(s)	1098
BsaBI	GATNNNNATC	1 cut(s)	888
BsaI	GGTCTC	1 cut(s)	368
BsaJI	CCNNGG	1 cut(s)	375
BsaWI	WCCGGW	1 cut(s)	825
Bsc4I	CCNNNNNNNGG	4 cut(s)	104, 146, 841, 1072
Bse118I	RCCGGY	1 cut(s)	849
Bse1I	ACTGG	2 cut(s)	107, 818
Bse8I	GATNNNNATC	1 cut(s)	888
BseBI	CCWGG	2 cut(s)	123, 376
BseCI	ATCGAT	1 cut(s)	1098
BseDI	CCNNGG	1 cut(s)	375
BseGI	GGATG	1 cut(s)	12
BseJI	GATNNNNATC	1 cut(s)	888
BseLI	CCNNNNNNNGG	4 cut(s)	104, 146, 841, 1072
BseMII	CTCAG	5 cut(s)	204, 351, 553, 716, 1053
BseNI	ACTGG	2 cut(s)	107, 818
BseRI	GAGGAG	1 cut(s)	886
BseSI	GKGCMC	1 cut(s)	806
BseXI	GCAGC	1 cut(s)	253
BseYI	CCCAGC	1 cut(s)	1007
BsgI	GTGCAG	1 cut(s)	1002
Bsh1285I	CGRYCG	1 cut(s)	892
BshFI	GGCC	6 cut(s)	126, 213, 548, 591, 849, 1016
BshNI	GGYRCC	1 cut(s)	896
BshVI	ATCGAT	1 cut(s)	1098
BsiEI	CGRYCG	1 cut(s)	892
BsiSI	CCGG	4 cut(s)	800, 826, 850, 1067
BslFI	GGGAC	2 cut(s)	740, 759
BslI	CCNNNNNNNGG	4 cut(s)	104, 146, 841, 1072
BsmAI	GTCTC	1 cut(s)	368
BsmFI	GGGAC	2 cut(s)	740, 759
BsmI	GAATGC	1 cut(s)	931
BsnI	GGCC	6 cut(s)	126, 213, 548, 591, 849, 1016
Bso31I	GGTCTC	1 cut(s)	368
Bsp1286I	GDGCHC	1 cut(s)	806
Bsp143I	GATC	5 cut(s)	856, 889, 1117, 1122, 1138
Bsp1720I	GCTNAGC	1 cut(s)	168
BspANI	GGCC	6 cut(s)	126, 213, 548, 591, 849, 1016
BspCNI	CTCAG	5 cut(s)	203, 350, 552, 715, 1052
BspDI	ATCGAT	1 cut(s)	1098
BspLI	GGNNCC	3 cut(s)	120, 727, 898
BspOI	GCTAGC	1 cut(s)	1091
BspPI	GGATC	1 cut(s)	1117
BspT107I	GGYRCC	1 cut(s)	896
BspTI	CTTAAG	1 cut(s)	707
BspTNI	GGTCTC	1 cut(s)	368
BsrFI	RCCGGY	1 cut(s)	849
BsrI	ACTGG	2 cut(s)	107, 818
BssAI	RCCGGY	1 cut(s)	849
BssECI	CCNNGG	1 cut(s)	375
BssMI	GATC	5 cut(s)	856, 889, 1117, 1122, 1138
Bst2UI	CCWGG	2 cut(s)	123, 376
Bst4CI	ACNGT	2 cut(s)	55, 606
Bst6I	CTCTTC	3 cut(s)	354, 505, 785
BstAFI	CTTAAG	1 cut(s)	707
BstC8I	GCNNGC	7 cut(s)	60, 165, 442, 847, 851, 1005, 1089
BstDEI	CTNAG	6 cut(s)	168, 190, 337, 539, 702, 1039
BstENI	CCTNNNNNAGG	1 cut(s)	144
BstF5I	GGATG	1 cut(s)	12
BstKTI	GATC	5 cut(s)	859, 892, 1120, 1125, 1141
BstMAI	GTCTC	1 cut(s)	368
BstMBI	GATC	5 cut(s)	856, 889, 1117, 1122, 1138
BstMCI	CGRYCG	1 cut(s)	892
BstMWI	GCNNNNNNNGC	4 cut(s)	68, 169, 778, 1013
BstNI	CCWGG	2 cut(s)	123, 376
BstSCI	CCNGG	4 cut(s)	121, 374, 799, 1065
BstSLI	GKGCMC	1 cut(s)	806
BstV1I	GCAGC	1 cut(s)	253
BstV2I	GAAGAC	2 cut(s)	519, 651
BstX2I	RGATCY	1 cut(s)	1122
BstYI	RGATCY	1 cut(s)	1122
Bsu15I	ATCGAT	1 cut(s)	1098
BsuI	GTATCC	1 cut(s)	77
BsuRI	GGCC	6 cut(s)	126, 213, 548, 591, 849, 1016
BsuTUI	ATCGAT	1 cut(s)	1098
BtsCI	GGATG	1 cut(s)	12
BtsIMutI	CAGTG	3 cut(s)	60, 423, 549
Cac8I	GCNNGC	7 cut(s)	60, 165, 442, 847, 851, 1005, 1089
CaiI	CAGNNNCTG	1 cut(s)	1136
Cfr10I	RCCGGY	1 cut(s)	849
Cfr13I	GGNCC	4 cut(s)	125, 176, 212, 1109
ClaI	ATCGAT	1 cut(s)	1098
Csp6I	GTAC	1 cut(s)	897
CviAII	CATG	5 cut(s)	173, 397, 457, 1115, 1180
CviQI	GTAC	1 cut(s)	897
DdeI	CTNAG	6 cut(s)	168, 190, 337, 539, 702, 1039
DpnI	GATC	5 cut(s)	858, 891, 1119, 1124, 1140
DpnII	GATC	5 cut(s)	856, 889, 1117, 1122, 1138
DriI	GACNNNNNGTC	1 cut(s)	752
EaeI	YGGCCR	2 cut(s)	546, 1014
Eam1104I	CTCTTC	3 cut(s)	354, 505, 785
Eam1105I	GACNNNNNGTC	1 cut(s)	752
EarI	CTCTTC	3 cut(s)	354, 505, 785
Eco31I	GGTCTC	1 cut(s)	368
Eco47I	GGWCC	2 cut(s)	176, 1109
Eco57I	CTGAAG	2 cut(s)	205, 1127
EcoNI	CCTNNNNNAGG	1 cut(s)	144
EcoRI	GAATTC	1 cut(s)	795
EcoRII	CCWGG	2 cut(s)	121, 374
FaeI	CATG	5 cut(s)	176, 400, 460, 1118, 1183
FalI	AAGNNNNNCTT	6 cut(s)	350, 382, 701, 733, 893, 925
FaqI	GGGAC	2 cut(s)	740, 759
FatI	CATG	5 cut(s)	172, 396, 456, 1114, 1179
FauNDI	CATATG	1 cut(s)	231
FbaI	TGATCA	2 cut(s)	856, 1117
FblI	GTMKAC	2 cut(s)	582, 822
Fnu4HI	GCNGC	1 cut(s)	242
FokI	GGATG	1 cut(s)	19
Fsp4HI	GCNGC	1 cut(s)	242
FspBI	CTAG	3 cut(s)	146, 437, 1088
GluI	GCNGC	1 cut(s)	242
GsaI	CCCAGC	1 cut(s)	1011
GsuI	CTGGAG	2 cut(s)	93, 951
HaeIII	GGCC	6 cut(s)	126, 213, 548, 591, 849, 1016
HapII	CCGG	4 cut(s)	800, 826, 850, 1067
Hin1II	CATG	5 cut(s)	176, 400, 460, 1118, 1183
HincII	GTYRAC	3 cut(s)	42, 393, 583
HindII	GTYRAC	3 cut(s)	42, 393, 583
HindIII	AAGCTT	1 cut(s)	567
HinfI	GANTC	5 cut(s)	75, 288, 473, 497, 935
HpaII	CCGG	4 cut(s)	800, 826, 850, 1067
HphI	GGTGA	4 cut(s)	336, 344, 364, 653
Hpy166II	GTNNAC	7 cut(s)	42, 393, 583, 823, 963, 1048, 1112
Hpy188I	TCNGA	5 cut(s)	542, 670, 861, 940, 1122
Hpy188III	TCNNGA	7 cut(s)	179, 292, 787, 968, 1059, 1136, 1163
Hpy8I	GTNNAC	7 cut(s)	42, 393, 583, 823, 963, 1048, 1112
HpyAV	CCTTC	4 cut(s)	108, 164, 231, 438
HpyCH4III	ACNGT	2 cut(s)	55, 606
HpyCH4V	TGCA	6 cut(s)	95, 266, 460, 479, 929, 983
HpyF10VI	GCNNNNNNNGC	4 cut(s)	68, 169, 778, 1013
HpyF3I	CTNAG	6 cut(s)	168, 190, 337, 539, 702, 1039
Hsp92II	CATG	5 cut(s)	176, 400, 460, 1118, 1183
KpnI	GGTACC	1 cut(s)	900
KroI	GCCGGC	1 cut(s)	849
KroNI	GCCGGC	1 cut(s)	851
Ksp22I	TGATCA	2 cut(s)	856, 1117
Kzo9I	GATC	5 cut(s)	856, 889, 1117, 1122, 1138
LmnI	GCTCC	1 cut(s)	1138
Lsp1109I	GCAGC	1 cut(s)	253
LweI	GCATC	1 cut(s)	253
MaeI	CTAG	3 cut(s)	146, 437, 1088
MaeIII	GTNAC	2 cut(s)	912, 985
MalI	GATC	5 cut(s)	858, 891, 1119, 1124, 1140
MboI	GATC	5 cut(s)	856, 889, 1117, 1122, 1138
MboII	GAAGA	7 cut(s)	211, 341, 492, 524, 656, 802, 1133
MflI	RGATCY	1 cut(s)	1122
MhlI	GDGCHC	1 cut(s)	806
MlsI	TGGCCA	1 cut(s)	548
MluCI	AATT	7 cut(s)	252, 296, 616, 795, 838, 904, 1151
MluNI	TGGCCA	1 cut(s)	548
MlyI	GAGTC	3 cut(s)	84, 491, 944
MmeI	TCCRAC	2 cut(s)	265, 528
MnlI	CCTC	7 cut(s)	352, 357, 542, 864, 887, 934, 1048
Mox20I	TGGCCA	1 cut(s)	548
MroNI	GCCGGC	1 cut(s)	849
MscI	TGGCCA	1 cut(s)	548
MseI	TTAA	1 cut(s)	708
Msp20I	TGGCCA	1 cut(s)	548
MspA1I	CMGCKG	1 cut(s)	1043
MspCI	CTTAAG	1 cut(s)	707
MspI	CCGG	4 cut(s)	800, 826, 850, 1067
MspR9I	CCNGG	4 cut(s)	123, 376, 801, 1067
Mva1269I	GAATGC	1 cut(s)	931
MvaI	CCWGG	2 cut(s)	123, 376
MwoI	GCNNNNNNNGC	4 cut(s)	68, 169, 778, 1013
NaeI	GCCGGC	1 cut(s)	851
NciI	CCSGG	2 cut(s)	801, 1067
NdeI	CATATG	1 cut(s)	231
NdeII	GATC	5 cut(s)	856, 889, 1117, 1122, 1138
NgoMIV	GCCGGC	1 cut(s)	849
NheI	GCTAGC	1 cut(s)	1087
NlaIII	CATG	5 cut(s)	176, 400, 460, 1118, 1183
NlaIV	GGNNCC	3 cut(s)	120, 727, 898
NmuCI	GTSAC	2 cut(s)	912, 985
PctI	GAATGC	1 cut(s)	931
PdiI	GCCGGC	1 cut(s)	851
PfeI	GAWTC	2 cut(s)	288, 473
PflMI	CCANNNNNTGG	1 cut(s)	841
PkrI	GCNGC	1 cut(s)	243
Ple19I	CGATCG	1 cut(s)	892
PleI	GAGTC	3 cut(s)	83, 491, 943
PpsI	GAGTC	3 cut(s)	83, 491, 943
PsiI	TTATAA	1 cut(s)	251
Psp6I	CCWGG	2 cut(s)	121, 374
PspFI	CCCAGC	1 cut(s)	1007
PspGI	CCWGG	2 cut(s)	121, 374
PspN4I	GGNNCC	3 cut(s)	120, 727, 898
PspPI	GGNCC	4 cut(s)	125, 176, 212, 1109
PstNI	CAGNNNCTG	1 cut(s)	1136
PsuI	RGATCY	1 cut(s)	1122
PvuI	CGATCG	1 cut(s)	892
PvuII	CAGCTG	1 cut(s)	1043
RsaI	GTAC	1 cut(s)	898
RsaNI	GTAC	1 cut(s)	897
SalI	GTCGAC	1 cut(s)	581
SaqAI	TTAA	1 cut(s)	708
SatI	GCNGC	1 cut(s)	242
Sau3AI	GATC	5 cut(s)	856, 889, 1117, 1122, 1138
Sau96I	GGNCC	4 cut(s)	125, 176, 212, 1109
SchI	GAGTC	3 cut(s)	84, 491, 944
ScrFI	CCNGG	4 cut(s)	123, 376, 801, 1067
SduI	GDGCHC	1 cut(s)	806
SfaNI	GCATC	1 cut(s)	253
SinI	GGWCC	2 cut(s)	176, 1109
SmlI	CTYRAG	1 cut(s)	707
SmoI	CTYRAG	1 cut(s)	707
Sse9I	AATT	7 cut(s)	252, 296, 616, 795, 838, 904, 1151
SspMI	CTAG	3 cut(s)	146, 437, 1088
StyD4I	CCNGG	4 cut(s)	121, 374, 799, 1065
TaaI	ACNGT	2 cut(s)	55, 606
TaqI	TCGA	3 cut(s)	582, 892, 1098
TasI	AATT	7 cut(s)	252, 296, 616, 795, 838, 904, 1151
TfiI	GAWTC	2 cut(s)	288, 473
Tru1I	TTAA	1 cut(s)	708
Tru9I	TTAA	1 cut(s)	708
TscAI	CASTG	3 cut(s)	60, 423, 556
TseFI	GTSAC	2 cut(s)	912, 985
TseI	GCWGC	1 cut(s)	241
Tsp45I	GTSAC	2 cut(s)	912, 985
TspDTI	ATGAA	7 cut(s)	218, 420, 675, 893, 938, 1008, 1196
TspRI	CASTG	3 cut(s)	60, 423, 556
Van91I	CCANNNNNTGG	1 cut(s)	841
Vha464I	CTTAAG	1 cut(s)	707
VpaK11BI	GGWCC	2 cut(s)	176, 1109
XagI	CCTNNNNNAGG	1 cut(s)	144
XapI	RAATTY	5 cut(s)	296, 795, 838, 904, 1151
XcmI	CCANNNNNNNNNTGG	1 cut(s)	31
XmiI	GTMKAC	2 cut(s)	582, 822
XspI	CTAG	3 cut(s)	146, 437, 1088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.