Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0004709

Physical Location & Seq

Genomic Coordinates Reverse (-)

3230126 .. 3232615

Gene Structure 2490 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g05420.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1206 bp

ATGATTGCTTGGAAGAAGAGGAGACCCTTCAGAAAATTGGTGGGGATGGAAGTGTTGCTTGCTACCAGTGTTTGGTTGATGTCCACAATCTTCACAGGGTTGGCAATATTGGGAGGAATTTTGGGGTACTTGTATGGGCCCTACTGGGGGGTGAGAAGGGTACCTGGCCCACCAACTATCCCCTTGCTAGGACACCTTCCCTTGCTGGCAAAGTATGGTCCTGATGTATTCTCAGTTCTTGCCGAACAGTACGGCCCTATTTTCAGGTTTCATATGGGCAGGCAACCATTGATAATTGTAGCAGATGCTGAGCTTTGTAGAGAAGTTGGAATAAAGAAATTCAAAGACATTCCGAACAGAAGCATTCCAGCTCCCATCTCAGCCTCCCCTCTTCATCAAAAGGGTCTTTTTTTCACTAGGGATGCAAGATGGTCGACAATGCGAAACACTATATTATCGGTGTATCAGCCATCACACCTCGCCAGCCTTGTGCCTACCATGCAGTCCTTCATTGAATCTGCAACTGAGAAACTGCACTCCTCCAAAGAAGAAGAGATTACATTTTCTACCCTTTCCCTCGGATTAGCTACTGATGTACTAGGGCAAGCTGCATTTGGTGTCAACTTTGGCCTTTCTAATACACATTCGATCGGCAAATCAATCAAAAAGATTGACGGAGAAGACAACGACGATGAAGTCTCGGACTTTATCAACCAACATATTTATTCCACAACGCAGCTTAAGATGGACTTCTCAGGTTCCTTATCTATCATACTCGGTTTACTAGTCCCCATACTCCAGGAGCCATTTCGGCAGATTTTGAAGAGAATTCCGGGCACTATGGACTGGAAAGTTGAGCGAACGAACCGGAAGTTGAGTGGCCGGGTTGACGATATTGTGGCGAAGAGAATGAAAGACCGTGAGCGAGGCTCGAAGGACTTCTTGTCGGTAATTATGAATGCAATGGAATCAGAGGCGGTTTCGAAGAATTTCTTCACTCCTGACTACATTAGTGCAGTTACTTACGAGCATCTCCTTGCTGGATCAGCCACCACAGCATTCACATTGTCTTCAATTGTCTACTTGGTTGCTGGACATCCAGAAGTCGAAAAAAAGTTGCTGGCAGAGATTGATGGATTTGGACCGCCTGATCGATCCCAACTGCTCATGATCTTCAACACAAGTTTCCTTACATTGATCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0006629 GO:0006720 GO:0006721 GO:0006810 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009914 GO:0009926 GO:0009934 GO:0009962 GO:0009963 GO:0009987 GO:0010016 GO:0010223 GO:0010346 GO:0010817 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0019222 GO:0022603 GO:0032501 GO:0032502 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0046148 GO:0048367 GO:0048509 GO:0048518 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:1901576 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

p450

Protein Analysis

402

Amino Acids

44.71

Weight (kDa)

9.18

Isoelectric Point (pI)

33.4

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004709)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26170 AT2G26170
fragaria_vesca	FvH4_2g31660 FvH4_2g31680 FvH4_2g31680 FvH4_2g31680 FvH4_2g31680 FvH4_2g31680
malus_domestica	MD02G1142400.v1.1 MD08G1064200.v1.1 MD15G1057500.v1.1 MD15G1057600.v1.1
prunus_persica	Prupe.1G410100_v2.0.a1 Prupe.1G410100_v2.0.a1 Prupe.1G410300_v2.0.a1 Prupe.1G410300_v2.0.a1 Prupe.1G410300_v2.0.a1
pyrus_communis	pycom08g05270 pycom15g05410 pycom15g05420 pycom15g22400
rosa_chinensis	RchiOBHm_Chr6g0311161
rosa_laevigata	RLG00000010431
rosa_multiflora	Rmu_sc0008967.1_g000014 Rmu_sc0016962.1_g000003
rosa_roxburghii	Rroxscaffold_7G00157950
rosa_rugosa	Rorug06G0386000
rosa_samantha	Rh6BG510700 Rh6CG516400 Rh6DG502900
rosa_wichuraiana	Rw6G043550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	695
Acc65I	GGTACC	1 cut(s)	160
AccB1I	GGYRCC	1 cut(s)	160
AccB7I	CCANNNNNTGG	1 cut(s)	72
AccI	GTMKAC	2 cut(s)	434, 1080
AciI	CCGC	2 cut(s)	977, 1145
AclWI	GGATC	2 cut(s)	1051, 1149
AcoI	YGGCCR	1 cut(s)	880
AcsI	RAATTY	4 cut(s)	117, 338, 828, 988
AcuI	CTGAAG	1 cut(s)	13
AfaI	GTAC	4 cut(s)	128, 162, 251, 597
AfiI	CCNNNNNNNGG	4 cut(s)	72, 146, 147, 188
AflII	CTTAAG	1 cut(s)	740
AgsI	TTSAA	5 cut(s)	343, 515, 823, 1074, 1177
AhdI	GACNNNNNGTC	1 cut(s)	943
AhlI	ACTAGT	1 cut(s)	784
AjnI	CCWGG	2 cut(s)	163, 798
AluBI	AGCT	5 cut(s)	313, 371, 587, 608, 739
AluI	AGCT	5 cut(s)	313, 371, 587, 608, 739
Alw26I	GTCTC	2 cut(s)	16, 703
AlwI	GGATC	2 cut(s)	1051, 1149
AlwNI	CAGNNNCTG	1 cut(s)	308
AoxI	GGCC	5 cut(s)	137, 166, 253, 628, 880
ApaI	GGGCCC	1 cut(s)	141
ApeKI	GCWGC	2 cut(s)	608, 736
ApoI	RAATTY	4 cut(s)	117, 338, 828, 988
Asp700I	GAANNNNTTC	3 cut(s)	938, 989, 992
Asp718I	GGTACC	1 cut(s)	160
AspS9I	GGNCC	6 cut(s)	137, 138, 167, 218, 254, 1142
AsuC2I	CCSGG	2 cut(s)	834, 884
AsuHPI	GGTGA	1 cut(s)	163
AsuII	TTCGAA	1 cut(s)	983
AvaII	GGWCC	2 cut(s)	218, 1142
BaeGI	GKGCMC	2 cut(s)	141, 839
BanI	GGYRCC	1 cut(s)	160
BanII	GRGCYC	1 cut(s)	141
BbsI	GAAGAC	2 cut(s)	687, 1062
BbvI	GCAGC	2 cut(s)	595, 748
BccI	CCATC	6 cut(s)	40, 383, 423, 478, 739, 1127
BceAI	ACGGC	1 cut(s)	268
BcgI	CGANNNNNNTGC	2 cut(s)	414, 448
BciT130I	CCWGG	2 cut(s)	165, 800
BclI	TGATCA	1 cut(s)	1197
BcnI	CCSGG	2 cut(s)	834, 884
BcoDI	GTCTC	2 cut(s)	16, 703
BcuI	ACTAGT	1 cut(s)	784
BfaI	CTAG	4 cut(s)	188, 417, 599, 785
BfrI	CTTAAG	1 cut(s)	740
BglI	GCCNNNNNGGC	1 cut(s)	811
BisI	GCNGC	2 cut(s)	609, 737
BlpI	GCTNAGC	1 cut(s)	309
BlsI	GCNGC	2 cut(s)	610, 738
Bme1390I	CCNGG	4 cut(s)	165, 800, 834, 884
Bme18I	GGWCC	2 cut(s)	218, 1142
BmeRI	GACNNNNNGTC	1 cut(s)	943
BmgT120I	GGNCC	6 cut(s)	137, 138, 167, 218, 254, 1142
BmiI	GGNNCC	4 cut(s)	139, 162, 760, 804
BmrFI	CCNGG	4 cut(s)	165, 800, 834, 884
BmrI	ACTGGG	1 cut(s)	154
BmsI	GCATC	3 cut(s)	295, 412, 1039
BmuI	ACTGGG	1 cut(s)	154
BpiI	GAAGAC	2 cut(s)	687, 1062
BplI	GAGNNNNNCTC	2 cut(s)	914, 946
BpmI	CTGGAG	1 cut(s)	782
Bpu1102I	GCTNAGC	1 cut(s)	309
Bpu14I	TTCGAA	1 cut(s)	983
BpuMI	CCSGG	2 cut(s)	834, 884
Bsa29I	ATCGAT	1 cut(s)	1153
BsaI	GGTCTC	1 cut(s)	16
BsaJI	CCNNGG	1 cut(s)	577
BsaWI	WCCGGW	1 cut(s)	867
Bsc4I	CCNNNNNNNGG	4 cut(s)	72, 146, 147, 188
Bse1I	ACTGG	3 cut(s)	66, 149, 851
Bse3DI	GCAATG	1 cut(s)	969
BseBI	CCWGG	2 cut(s)	165, 800
BseCI	ATCGAT	1 cut(s)	1153
BseDI	CCNNGG	1 cut(s)	577
BseGI	GGATG	3 cut(s)	51, 427, 1096
BseLI	CCNNNNNNNGG	4 cut(s)	72, 146, 147, 188
BseMI	GCAATG	1 cut(s)	969
BseMII	CTCAG	5 cut(s)	246, 300, 393, 516, 768
BseNI	ACTGG	3 cut(s)	66, 149, 851
BseRI	GAGGAG	2 cut(s)	34, 529
BseSI	GKGCMC	2 cut(s)	141, 839
BseXI	GCAGC	2 cut(s)	595, 748
BsgI	GTGCAG	2 cut(s)	518, 1035
Bsh1285I	CGRYCG	1 cut(s)	651
BshFI	GGCC	5 cut(s)	139, 168, 255, 630, 882
BshNI	GGYRCC	1 cut(s)	160
BshVI	ATCGAT	1 cut(s)	1153
BsiEI	CGRYCG	1 cut(s)	651
BsiSI	CCGG	3 cut(s)	833, 868, 883
BslFI	GGGAC	1 cut(s)	773
BslI	CCNNNNNNNGG	4 cut(s)	72, 146, 147, 188
BsmAI	GTCTC	2 cut(s)	16, 703
BsmFI	GGGAC	1 cut(s)	773
BsmI	GAATGC	3 cut(s)	363, 964, 1058
BsnI	GGCC	5 cut(s)	139, 168, 255, 630, 882
Bso31I	GGTCTC	1 cut(s)	16
Bsp119I	TTCGAA	1 cut(s)	983
Bsp120I	GGGCCC	1 cut(s)	137
Bsp1286I	GDGCHC	2 cut(s)	141, 839
Bsp143I	GATC	6 cut(s)	648, 1043, 1150, 1154, 1170, 1197
Bsp1720I	GCTNAGC	1 cut(s)	309
BspACI	CCGC	2 cut(s)	977, 1145
BspANI	GGCC	5 cut(s)	139, 168, 255, 630, 882
BspCNI	CTCAG	5 cut(s)	245, 301, 392, 517, 767
BspDI	ATCGAT	1 cut(s)	1153
BspHI	TCATGA	1 cut(s)	1167
BspLI	GGNNCC	4 cut(s)	139, 162, 760, 804
BspPI	GGATC	2 cut(s)	1051, 1149
BspT104I	TTCGAA	1 cut(s)	983
BspT107I	GGYRCC	1 cut(s)	160
BspTI	CTTAAG	1 cut(s)	740
BspTNI	GGTCTC	1 cut(s)	16
BsrDI	GCAATG	1 cut(s)	969
BsrI	ACTGG	3 cut(s)	66, 149, 851
BssECI	CCNNGG	1 cut(s)	577
BssMI	GATC	6 cut(s)	648, 1043, 1150, 1154, 1170, 1197
Bst2UI	CCWGG	2 cut(s)	165, 800
Bst4CI	ACNGT	2 cut(s)	249, 920
Bst6I	CTCTTC	5 cut(s)	11, 396, 546, 818, 899
BstAFI	CTTAAG	1 cut(s)	740
BstBI	TTCGAA	1 cut(s)	983
BstC8I	GCNNGC	6 cut(s)	60, 207, 281, 484, 606, 1122
BstDEI	CTNAG	5 cut(s)	232, 309, 379, 525, 754
BstF5I	GGATG	3 cut(s)	51, 427, 1096
BstKTI	GATC	6 cut(s)	651, 1046, 1153, 1157, 1173, 1200
BstMAI	GTCTC	2 cut(s)	16, 703
BstMBI	GATC	6 cut(s)	648, 1043, 1150, 1154, 1170, 1197
BstMCI	CGRYCG	1 cut(s)	651
BstMWI	GCNNNNNNNGC	4 cut(s)	499, 811, 1046, 1055
BstNI	CCWGG	2 cut(s)	165, 800
BstSCI	CCNGG	4 cut(s)	163, 798, 832, 882
BstSLI	GKGCMC	2 cut(s)	141, 839
BstV1I	GCAGC	2 cut(s)	595, 748
BstV2I	GAAGAC	2 cut(s)	687, 1062
Bsu15I	ATCGAT	1 cut(s)	1153
BsuRI	GGCC	5 cut(s)	139, 168, 255, 630, 882
BsuTUI	ATCGAT	1 cut(s)	1153
BtsCI	GGATG	3 cut(s)	51, 427, 1096
BtsIMutI	CAGTG	1 cut(s)	73
Cac8I	GCNNGC	6 cut(s)	60, 207, 281, 484, 606, 1122
CaiI	CAGNNNCTG	1 cut(s)	308
CciI	TCATGA	1 cut(s)	1167
Cfr13I	GGNCC	6 cut(s)	137, 138, 167, 218, 254, 1142
ClaI	ATCGAT	1 cut(s)	1153
Csp6I	GTAC	4 cut(s)	127, 161, 250, 596
CviAII	CATG	2 cut(s)	499, 1168
CviQI	GTAC	4 cut(s)	127, 161, 250, 596
DdeI	CTNAG	5 cut(s)	232, 309, 379, 525, 754
DpnI	GATC	6 cut(s)	650, 1045, 1152, 1156, 1172, 1199
DpnII	GATC	6 cut(s)	648, 1043, 1150, 1154, 1170, 1197
DrdI	GACNNNNNNGTC	1 cut(s)	695
DriI	GACNNNNNGTC	1 cut(s)	943
DseDI	GACNNNNNNGTC	1 cut(s)	695
EaeI	YGGCCR	1 cut(s)	880
Eam1104I	CTCTTC	5 cut(s)	11, 396, 546, 818, 899
Eam1105I	GACNNNNNGTC	1 cut(s)	943
EarI	CTCTTC	5 cut(s)	11, 396, 546, 818, 899
Eco24I	GRGCYC	1 cut(s)	141
Eco31I	GGTCTC	1 cut(s)	16
Eco47I	GGWCC	2 cut(s)	218, 1142
Eco57I	CTGAAG	1 cut(s)	13
EcoO109I	RGGNCCY	1 cut(s)	138
EcoRI	GAATTC	1 cut(s)	828
EcoRII	CCWGG	2 cut(s)	163, 798
EcoT38I	GRGCYC	1 cut(s)	141
FaeI	CATG	2 cut(s)	502, 1171
FalI	AAGNNNNNCTT	8 cut(s)	42, 74, 734, 766, 926, 958, 977, 1009
FaqI	GGGAC	1 cut(s)	773
FatI	CATG	2 cut(s)	498, 1167
FauNDI	CATATG	1 cut(s)	273
FbaI	TGATCA	1 cut(s)	1197
FblI	GTMKAC	2 cut(s)	434, 1080
Fnu4HI	GCNGC	2 cut(s)	609, 737
FokI	GGATG	3 cut(s)	58, 434, 1083
FriOI	GRGCYC	1 cut(s)	141
Fsp4HI	GCNGC	2 cut(s)	609, 737
FspBI	CTAG	4 cut(s)	188, 417, 599, 785
GluI	GCNGC	2 cut(s)	609, 737
GsuI	CTGGAG	1 cut(s)	782
HaeIII	GGCC	5 cut(s)	139, 168, 255, 630, 882
HapII	CCGG	3 cut(s)	833, 868, 883
Hin1II	CATG	2 cut(s)	502, 1171
HincII	GTYRAC	3 cut(s)	435, 622, 889
HindII	GTYRAC	3 cut(s)	435, 622, 889
HinfI	GANTC	2 cut(s)	515, 968
HpaII	CCGG	3 cut(s)	833, 868, 883
HphI	GGTGA	1 cut(s)	163
Hpy166II	GTNNAC	6 cut(s)	84, 435, 622, 782, 889, 1081
Hpy188I	TCNGA	5 cut(s)	32, 354, 581, 703, 973
Hpy188III	TCNNGA	4 cut(s)	221, 1001, 1100, 1168
Hpy8I	GTNNAC	6 cut(s)	84, 435, 622, 782, 889, 1081
Hpy99I	CGWCG	1 cut(s)	692
HpyAV	CCTTC	5 cut(s)	37, 150, 206, 517, 928
HpyCH4III	ACNGT	2 cut(s)	249, 920
HpyCH4V	TGCA	7 cut(s)	425, 502, 521, 535, 611, 962, 1016
HpyF10VI	GCNNNNNNNGC	4 cut(s)	499, 811, 1046, 1055
HpyF3I	CTNAG	5 cut(s)	232, 309, 379, 525, 754
Hsp92II	CATG	2 cut(s)	502, 1171
KpnI	GGTACC	1 cut(s)	164
Ksp22I	TGATCA	1 cut(s)	1197
Kzo9I	GATC	6 cut(s)	648, 1043, 1150, 1154, 1170, 1197
LmnI	GCTCC	2 cut(s)	376, 802
Lsp1109I	GCAGC	2 cut(s)	595, 748
LweI	GCATC	3 cut(s)	295, 412, 1039
MaeI	CTAG	4 cut(s)	188, 417, 599, 785
MaeIII	GTNAC	1 cut(s)	1018
MalI	GATC	6 cut(s)	650, 1045, 1152, 1156, 1172, 1199
MboI	GATC	6 cut(s)	648, 1043, 1150, 1154, 1170, 1197
MfeI	CAATTG	1 cut(s)	1074
MhlI	GDGCHC	2 cut(s)	141, 839
MluCI	AATT	8 cut(s)	35, 117, 294, 338, 828, 951, 988, 1074
MmeI	TCCRAC	1 cut(s)	307
MnlI	CCTC	9 cut(s)	12, 107, 394, 399, 488, 550, 587, 920, 967
MroXI	GAANNNNTTC	3 cut(s)	938, 989, 992
MseI	TTAA	1 cut(s)	741
MspCI	CTTAAG	1 cut(s)	740
MspI	CCGG	3 cut(s)	833, 868, 883
MspR9I	CCNGG	4 cut(s)	165, 800, 834, 884
MunI	CAATTG	1 cut(s)	1074
Mva1269I	GAATGC	3 cut(s)	363, 964, 1058
MvaI	CCWGG	2 cut(s)	165, 800
MwoI	GCNNNNNNNGC	4 cut(s)	499, 811, 1046, 1055
NciI	CCSGG	2 cut(s)	834, 884
NdeI	CATATG	1 cut(s)	273
NdeII	GATC	6 cut(s)	648, 1043, 1150, 1154, 1170, 1197
NlaIII	CATG	2 cut(s)	502, 1171
NlaIV	GGNNCC	4 cut(s)	139, 162, 760, 804
NspV	TTCGAA	1 cut(s)	983
PagI	TCATGA	1 cut(s)	1167
PctI	GAATGC	3 cut(s)	363, 964, 1058
PdmI	GAANNNNTTC	3 cut(s)	938, 989, 992
PfeI	GAWTC	2 cut(s)	515, 968
PflMI	CCANNNNNTGG	1 cut(s)	72
PfoI	TCCNGGA	1 cut(s)	798
PkrI	GCNGC	2 cut(s)	610, 738
Ple19I	CGATCG	1 cut(s)	651
Psp6I	CCWGG	2 cut(s)	163, 798
PspGI	CCWGG	2 cut(s)	163, 798
PspN4I	GGNNCC	4 cut(s)	139, 162, 760, 804
PspOMI	GGGCCC	1 cut(s)	137
PspPI	GGNCC	6 cut(s)	137, 138, 167, 218, 254, 1142
PsrI	GAACNNNNNNTAC	2 cut(s)	219, 251
PstNI	CAGNNNCTG	1 cut(s)	308
PvuI	CGATCG	1 cut(s)	651
RsaI	GTAC	4 cut(s)	128, 162, 251, 597
RsaNI	GTAC	4 cut(s)	127, 161, 250, 596
SalI	GTCGAC	1 cut(s)	433
SaqAI	TTAA	1 cut(s)	741
SatI	GCNGC	2 cut(s)	609, 737
Sau3AI	GATC	6 cut(s)	648, 1043, 1150, 1154, 1170, 1197
Sau96I	GGNCC	6 cut(s)	137, 138, 167, 218, 254, 1142
ScrFI	CCNGG	4 cut(s)	165, 800, 834, 884
SduI	GDGCHC	2 cut(s)	141, 839
SfaNI	GCATC	3 cut(s)	295, 412, 1039
SfuI	TTCGAA	1 cut(s)	983
SinI	GGWCC	2 cut(s)	218, 1142
SmlI	CTYRAG	1 cut(s)	740
SmoI	CTYRAG	1 cut(s)	740
SpeI	ACTAGT	1 cut(s)	784
Sse9I	AATT	8 cut(s)	35, 117, 294, 338, 828, 951, 988, 1074
SsiI	CCGC	2 cut(s)	977, 1145
SspI	AATATT	1 cut(s)	108
SspMI	CTAG	4 cut(s)	188, 417, 599, 785
StyD4I	CCNGG	4 cut(s)	163, 798, 832, 882
TaaI	ACNGT	2 cut(s)	249, 920
TaqI	TCGA	6 cut(s)	434, 647, 932, 983, 1107, 1153
TasI	AATT	8 cut(s)	35, 117, 294, 338, 828, 951, 988, 1074
TatI	WGTACW	1 cut(s)	595
TfiI	GAWTC	2 cut(s)	515, 968
Tru1I	TTAA	1 cut(s)	741
Tru9I	TTAA	1 cut(s)	741
TscAI	CASTG	1 cut(s)	73
TseI	GCWGC	2 cut(s)	608, 736
TspDTI	ATGAA	6 cut(s)	260, 383, 499, 708, 926, 971
TspGWI	ACGGA	1 cut(s)	690
TspRI	CASTG	1 cut(s)	73
Van91I	CCANNNNNTGG	1 cut(s)	72
Vha464I	CTTAAG	1 cut(s)	740
VpaK11BI	GGWCC	2 cut(s)	218, 1142
XapI	RAATTY	4 cut(s)	117, 338, 828, 988
XmiI	GTMKAC	2 cut(s)	434, 1080
XmnI	GAANNNNTTC	3 cut(s)	938, 989, 992
XspI	CTAG	4 cut(s)	188, 417, 599, 785

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.