FvH4_2g33330

Glycine-rich cell wall structural

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0018385
Physical Location & Seq
Reverse (-)
25030445 .. 25034226
3782 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33330.t1

Sequence Viewer

Length: 648 bp
ATGGGAAGGATTGAGGTTTCATGGAGTCTCATTTTCTTGGCGATGGGGATGTTTAGTTGTGTATCTGTTGAAGCAACGGGGAGGTCCCTGAGAGATAAGGGAAATAAATTGGACAAGGGGCTCGAGAACTGGCACGGTAAGGAGTCTAGCTATGGAGAGATGACAGCTGGTGGCTTCGGGGGAGGGTCTTATAACAGAGATGAACTGGACAAGGGGCTCCAGAGTTGGCGCGGTAAGGAGACTACCTATGAAGAACTGACAGCCGGTGGTTACGGAGGCGGGTCTGGTTACGGGTCAGGTGGTAGTGGGGGTTACGGCAGTGGGGGTGGTGTAGGCAATGGTGGCAGTGGCTTTGGAGGTGGTGGTGGTTATAGCGGGGGATCTGGTAGTGGTGGTGGATTTGGAAATGGTGGTGGAGGTTATGGTACTGGTGGCGGATTTGGCAATGGCGGTGGGGGTGGTGGATTCGGTAATGGTGGGGGATATGGCAATGGAGGTGGATCCGGAGGAGGTGGTGGCTTTGGCAATGTTAGTGGAGGAAGTGGACATGGGGGAGGCTTTGGCGTCGGGTCAGGTCCAGGGTATGCTGGCCGGGGTGGTGGCGGCGGGGGTGGCGGGGGATATGATCATGATGGGAAGAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

216

Amino Acids

19.85

Weight (kDa)

6.28

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018385)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33330
malus_domestica MD08G1087800.v1.1
prunus_persica Prupe.1G425300_v2.0.a1
pyrus_communis pycom15g06790
rosa_chinensis RchiOBHm_Chr6g0309291
rosa_laevigata RLG00000010576
rosa_multiflora Rmu_sc0015692.1_g000005
rosa_rugosa Rorug06G0370700
rosa_samantha Rh6AG484100
rosa_wichuraiana Rw6G042140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 192
AccII CGCG 1 cut(s) 231
AccIII TCCGGA 1 cut(s) 503
AciI CCGC 8 cut(s) 231, 279, 375, 435, 450, 603, 606, 615
AclWI GGATC 3 cut(s) 388, 495, 508
AcoI YGGCCR 1 cut(s) 589
AcyI GRCGYC 1 cut(s) 564
AfaI GTAC 1 cut(s) 427
AgsI TTSAA 1 cut(s) 71
AjnI CCWGG 1 cut(s) 577
AluBI AGCT 2 cut(s) 150, 167
AluI AGCT 2 cut(s) 150, 167
Alw26I GTCTC 2 cut(s) 32, 233
AlwI GGATC 3 cut(s) 388, 495, 508
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 503
AoxI GGCC 1 cut(s) 589
AspLEI GCGC 1 cut(s) 231
AspS9I GGNCC 2 cut(s) 84, 575
AsuC2I CCSGG 1 cut(s) 593
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 2 cut(s) 84, 575
BamHI GGATCC 1 cut(s) 500
BanII GRGCYC 2 cut(s) 123, 219
BccI CCATC 2 cut(s) 37, 626
BceAI ACGGC 1 cut(s) 331
BciT130I CCWGG 1 cut(s) 579
BclI TGATCA 1 cut(s) 625
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 2 cut(s) 32, 233
BfaI CTAG 1 cut(s) 147
BisI GCNGC 1 cut(s) 604
BlsI GCNGC 1 cut(s) 605
Bme1390I CCNGG 2 cut(s) 579, 593
Bme18I GGWCC 2 cut(s) 84, 575
BmeT110I CYCGRG 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 84, 575
BmiI GGNNCC 3 cut(s) 86, 218, 502
BmrFI CCNGG 2 cut(s) 579, 593
BpmI CTGGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 593
BsaHI GRCGYC 1 cut(s) 564
BsaJI CCNNGG 2 cut(s) 578, 592
BsaWI WCCGGW 1 cut(s) 503
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 134, 210, 433
Bse3DI GCAATG 4 cut(s) 343, 451, 496, 532
BseAI TCCGGA 1 cut(s) 503
BseBI CCWGG 1 cut(s) 579
BseDI CCNNGG 2 cut(s) 578, 592
BseGI GGATG 1 cut(s) 54
BseMI GCAATG 4 cut(s) 343, 451, 496, 532
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 3 cut(s) 134, 210, 433
BseRI GAGGAG 1 cut(s) 522
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 3 cut(s) 264, 504, 592
BslFI GGGAC 1 cut(s) 70
BsmAI GTCTC 2 cut(s) 32, 233
BsmFI GGGAC 1 cut(s) 70
BsnI GGCC 1 cut(s) 591
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 123, 219
Bsp13I TCCGGA 1 cut(s) 503
Bsp143I GATC 3 cut(s) 380, 500, 625
BspACI CCGC 8 cut(s) 231, 279, 375, 435, 450, 603, 606, 615
BspANI GGCC 1 cut(s) 591
BspCNI CTCAG 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 503
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 628
BspLI GGNNCC 3 cut(s) 86, 218, 502
BspPI GGATC 3 cut(s) 388, 495, 508
BsrDI GCAATG 4 cut(s) 343, 451, 496, 532
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 3 cut(s) 134, 210, 433
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 578, 592
BssMI GATC 3 cut(s) 380, 500, 625
BssNI GRCGYC 1 cut(s) 564
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 1 cut(s) 137
BstACI GRCGYC 1 cut(s) 564
BstC8I GCNNGC 1 cut(s) 589
BstDEI CTNAG 1 cut(s) 89
BstF5I GGATG 1 cut(s) 54
BstFNI CGCG 1 cut(s) 231
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 3 cut(s) 383, 503, 628
BstMAI GTCTC 2 cut(s) 32, 233
BstMBI GATC 3 cut(s) 380, 500, 625
BstMWI GCNNNNNNNGC 3 cut(s) 342, 441, 612
BstNI CCWGG 1 cut(s) 579
BstSCI CCNGG 2 cut(s) 577, 591
BstUI CGCG 1 cut(s) 231
BstX2I RGATCY 2 cut(s) 380, 500
BstYI RGATCY 2 cut(s) 380, 500
BsuRI GGCC 1 cut(s) 591
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 1 cut(s) 54
BtsI GCAGTG 2 cut(s) 325, 352
BtsIMutI CAGTG 2 cut(s) 325, 352
Cac8I GCNNGC 1 cut(s) 589
CciI TCATGA 1 cut(s) 628
CfoI GCGC 1 cut(s) 231
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 2 cut(s) 84, 575
CseI GACGC 1 cut(s) 553
Csp6I GTAC 1 cut(s) 426
CspCI CAANNNNNGTGG 6 cut(s) 433, 468, 478, 513, 514, 549
CviAII CATG 3 cut(s) 21, 548, 629
CviQI GTAC 1 cut(s) 426
DdeI CTNAG 1 cut(s) 89
DpnI GATC 3 cut(s) 382, 502, 627
DpnII GATC 3 cut(s) 380, 500, 625
EaeI YGGCCR 1 cut(s) 589
EciI GGCGGA 1 cut(s) 450
Eco24I GRGCYC 2 cut(s) 123, 219
Eco47I GGWCC 2 cut(s) 84, 575
Eco88I CYCGRG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 577
EcoT38I GRGCYC 2 cut(s) 123, 219
FaeI CATG 3 cut(s) 24, 551, 632
FaqI GGGAC 1 cut(s) 70
FatI CATG 3 cut(s) 20, 547, 628
FauI CCCGC 4 cut(s) 272, 368, 599, 608
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 1 cut(s) 604
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 2 cut(s) 123, 219
Fsp4HI GCNGC 1 cut(s) 604
FspBI CTAG 1 cut(s) 147
GlaI GCGC 1 cut(s) 230
GluI GCNGC 1 cut(s) 604
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 1 cut(s) 591
HapII CCGG 3 cut(s) 264, 504, 592
HgaI GACGC 1 cut(s) 553
HhaI GCGC 1 cut(s) 231
Hin1I GRCGYC 1 cut(s) 564
Hin1II CATG 3 cut(s) 24, 551, 632
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HinfI GANTC 3 cut(s) 25, 143, 465
HpaII CCGG 3 cut(s) 264, 504, 592
Hpy166II GTNNAC 1 cut(s) 545
Hpy188III TCNNGA 4 cut(s) 124, 220, 504, 629
Hpy8I GTNNAC 1 cut(s) 545
Hpy99I CGWCG 1 cut(s) 569
HpyCH4III ACNGT 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 3 cut(s) 342, 441, 612
HpyF3I CTNAG 1 cut(s) 89
Hsp92I GRCGYC 1 cut(s) 564
Hsp92II CATG 3 cut(s) 24, 551, 632
HspAI GCGC 1 cut(s) 229
Kpn2I TCCGGA 1 cut(s) 503
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 3 cut(s) 380, 500, 625
LmnI GCTCC 1 cut(s) 222
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 269, 287, 311
MalI GATC 3 cut(s) 382, 502, 627
MboI GATC 3 cut(s) 380, 500, 625
MboII GAAGA 1 cut(s) 263
MflI RGATCY 2 cut(s) 380, 500
MhlI GDGCHC 2 cut(s) 123, 219
MluCI AATT 1 cut(s) 107
MlyI GAGTC 2 cut(s) 34, 152
MroI TCCGGA 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 3 cut(s) 264, 504, 592
MspR9I CCNGG 2 cut(s) 579, 593
MvaI CCWGG 1 cut(s) 579
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 3 cut(s) 342, 441, 612
NciI CCSGG 1 cut(s) 593
NdeII GATC 3 cut(s) 380, 500, 625
NlaIII CATG 3 cut(s) 24, 551, 632
NlaIV GGNNCC 3 cut(s) 86, 218, 502
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 1 cut(s) 628
PfeI GAWTC 1 cut(s) 465
PkrI GCNGC 1 cut(s) 605
PleI GAGTC 2 cut(s) 33, 151
PpsI GAGTC 2 cut(s) 33, 151
PpuMI RGGWCCY 1 cut(s) 84
PsiI TTATAA 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 577
PspN4I GGNNCC 3 cut(s) 86, 218, 502
PspPI GGNCC 2 cut(s) 84, 575
PspPPI RGGWCCY 1 cut(s) 84
PsuI RGATCY 2 cut(s) 380, 500
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 1 cut(s) 427
RsaNI GTAC 1 cut(s) 426
SatI GCNGC 1 cut(s) 604
Sau3AI GATC 3 cut(s) 380, 500, 625
Sau96I GGNCC 2 cut(s) 84, 575
SchI GAGTC 2 cut(s) 34, 152
ScrFI CCNGG 2 cut(s) 579, 593
SduI GDGCHC 2 cut(s) 123, 219
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 2 cut(s) 84, 575
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 107
SsiI CCGC 8 cut(s) 231, 279, 375, 435, 450, 603, 606, 615
SspMI CTAG 1 cut(s) 147
StyD4I CCNGG 2 cut(s) 577, 591
TaaI ACNGT 1 cut(s) 137
TaqI TCGA 1 cut(s) 123
TasI AATT 1 cut(s) 107
TauI GCSGC 1 cut(s) 606
TfiI GAWTC 1 cut(s) 465
TscAI CASTG 2 cut(s) 325, 352
TspDTI ATGAA 3 cut(s) 9, 216, 264
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 2 cut(s) 325, 352
VpaK11BI GGWCC 2 cut(s) 84, 575
XhoI CTCGAG 1 cut(s) 122
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.