FvH4_2g34860

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005367
Physical Location & Seq
Reverse (-)
25809445 .. 25817306
7862 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34860.t4

Sequence Viewer

Length: 2730 bp
ATGGGAGTCGATATAAGGATGAGTCTGTCTTTGGGTTTTAATGTATTTGTCACTTCTGTCCTCTTTCTTCTTTTCTTCCATATTGTATTTATTGGCCTTTGGTACATCGGTCTTGTGTCACGTGTAGCTGGAAGGAGGCCAGCAATTTTGACAATCCTCCAAAACTGTGCTGTTCTTAGCGTAGCCTGCTGTATATTTTACAGTCATTGTGGCAACCGAGCTGTCCTGAGAGAGAAACAACTGGAACGAAGAAATTCTTTGTCTTGGTTTAATTTCTGGAAGAAAGATGAGAGAAATACATGGCTTTCTAAATTCCTTCGAATGAATGAGCTGAAAGATGAGGTCTGCTCATATTGGTTTGCTCCGGTGGGATCTGCTAGTGATTATCCTCTTTTGTCCAAGTGGGTCATTTACGGTGAGGTACAGGTAGAAAAATTAGCATGCAATGGTTCATGTGATGGTTCGTTGGATGGAATATCCCCATTATACTCATTATGGGCCACATTCATAGGCCTGTATATTGCTAATTATGTGGTGGAGAGGTCAACTGGGTGGGCTCTTACTCATCCTTTGTCTTTAGAAGAACATGAGAAGTCAAAGAACAAGCAAATGAAGCCTGATTTTTTGGATATGGTTCCTTGGTATTCAGGAACATCAGCTGATTTATTCAAGACTGTTTTTGACCTCCTGGTATCAGTAACTGTCTTTGTTGGCCGGTTTGACATGCGTATGATGCAAGCAGCAATGAGCAAGATCAGTGATGGAGCTTCCCAAGGGGATCTTTTATTTGATGATTTTGTTGAGAAGGATGGTCTATGGTTTGACTTCATGGCTGATACTGGCGATGGTGGTAACTCGTCGTATTCTGTGGCTCGGCTTCTTGCTCAGCCTTCCATCAATGTTAGCAAAGATGATTCTGTACTTAACCTACCACGTGGAGACCTATTACTTATTGGAGGAGATCTTGCGTATCCTAATCCATCATCCTTCACCTATGAAAGGCGTCTCTTTTGTCCGTTTGAGTATGCTCTTCAGCCCCCTCCATGGTCTAAACAAGATCATATAGCTGTAGATAAACCTGAGCTACCTTGTGGTGTGTCTGAACTGAAGCAATATGACGGGCCCCAGTGTTTTGTCATTCCTGGAAACCATGATTGGTTCGATGGACTTAATACATTTATGAGGTATATATGTCATAAGAGCTGGTTGGGTGGGTGGCTTATGCCTCAAAAGAAGAGTTATTTTGCTCTGAAACTCCCTAAAAGATGGTGGGTGTTTGGTCTTGATCTGGCACTCCACGGCGATATTGATGTATACCAATTTAAATTCTTCTCAGAACTAGTTAAAAACAAGGTAGGTGAAGATGATTCTGTAATCATTATGACACATGAGCCAAACTGGCTTCTTGACTGGTACTGGAATGATGTTTCTGGAAAGAATGTCGCGCATCTCATATGCGATCATTTGAAAGGAAGGTGTAAGCTACGAGTGGCTGGAGATTTGCATCATTATATGCGTCATTCATTTGTTAGGTCAGGGGATCCAATTCAAGTGCAACATTTACTTGTAAATGGTTGTGGTGGGGCTTTTCTACATCCCACACATGTATTTAGTAATTTTAAGAAACTTTATGGAGCATCGTATGAGACCAAGGCTGCTTATCCTTCATTTGAAGATTCAAGTAGGATTGCTTTAGGAAATATTTTAAAGTTTCGGAAGAAGAACTGGCAATTTGATTTCATTGGTGGCATTATATACTTTCTATTGGTCTTTTCTATGTTCCCTCAGTGTAAGCTCGATCATATCTTGCGAGAGGACTCATTTCCTGGTCACTTCAAGTCTTTCTTTGGAACAGTCTGGAATGCTTTTGTGTACATGCTGGAGCGCTCTTATGTGTCGTTTGCAGGTGCTGTTGTGTTGCTGATTGTGGCAATTACATTTGTCCCGTCGAAAGTGTCTCGGAAGAAACGGGTCATGATTGGAGTTCTTCATGTGTTTGCGCACCTGGCCGCAGCTCTGATTCTTATGTTGCTGTTGGAACTGGGTGTTGAGATGTGTGTCCAGCATCAACTACTAGGAACTTCAGGTTATCACACATTATATGAGTGGTACCGGACAGCTGAGAGTGAGCACTTTCCTGATCCAACAGGCCTTCGTGCTCGTATAGAACAGTGGACGTTCGGTCTTTATCCAGCATGCATCAAGTATTTCATGTCTGCATTTGATGTTCCAGAGGTTATGGCAGTCACCCGGAACAATATATGCAAGAATGGGATGGAATCACTCTCTAGATTGGGTGCTTGTATATATTATGCTTCTGTCTTCCTTTATTTTTGGGTCTTCTCTACTCCAGTGGTTTCTTTGGTTTTCGGCAGCTACCTATATGTTTGCATTAACTGGTTTCACATACACTTTGACGAAGCGTTCTCCTCCTTGCGAATTGCTAATTACAAGTCATTCACTCGGTTCCACATCAATACTGATGGGGATCTTGAAGTGTATACTCTTGCAGTTGATAAGGTTCCGAAAGAATGGAAGCTGGACCCTGATTGGGATGCGGAGCCAAAACAGCCACAACAGATGAGCCACAGGAGGAAGTTTCCTAGTAAATGGAGTGCAAAAGCTGCTCAGCAAGACCCACTACATAATGTTAAGATAGTTGATCACTTTGTAATTCGACAAACAGATAACACTGATATAGCAGGAAGTGATGGGTCAGTAATTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

910

Amino Acids

104.07

Weight (kDa)

7.15

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 391 - 591 1.1e-06 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1895
Acc16I TGCGCA 1 cut(s) 2001
Acc36I ACCTGC 1 cut(s) 1895
Acc65I GGTACC 1 cut(s) 2109
AccB1I GGYRCC 1 cut(s) 2109
AccI GTMKAC 2 cut(s) 1314, 2501
AccII CGCG 1 cut(s) 1445
AciI CCGC 2 cut(s) 2010, 2558
AclWI GGATC 6 cut(s) 379, 786, 1535, 1548, 2135, 2496
AcoI YGGCCR 2 cut(s) 712, 2007
AcsI RAATTY 3 cut(s) 253, 311, 1325
AcuI CTGAAG 3 cut(s) 1016, 1127, 2067
AcvI CACGTG 2 cut(s) 122, 935
AcyI GRCGYC 1 cut(s) 1003
AfaI GTAC 6 cut(s) 104, 423, 921, 1415, 1874, 2111
AfeI AGCGCT 1 cut(s) 1886
AfiI CCNNNNNNNGG 3 cut(s) 999, 1044, 2551
AflIII ACRYGT 2 cut(s) 121, 1603
AgsI TTSAA 7 cut(s) 670, 1468, 1550, 1673, 1680, 1837, 2495
AhdI GACNNNNNGTC 1 cut(s) 2181
AhlI ACTAGT 1 cut(s) 1339
AjnI CCWGG 4 cut(s) 687, 1141, 1825, 2004
AjuI GAANNNNNNNTTGG 2 cut(s) 1138, 1170
Alw21I GWGCWC 2 cut(s) 2133, 2161
Alw26I GTCTC 4 cut(s) 933, 1010, 1640, 1962
AlwI GGATC 6 cut(s) 379, 786, 1535, 1548, 2135, 2496
AlwNI CAGNNNCTG 2 cut(s) 701, 1910
Aor51HI AGCGCT 1 cut(s) 1886
AoxI GGCC 8 cut(s) 94, 137, 498, 511, 712, 1121, 2007, 2149
ApaI GGGCCC 1 cut(s) 1125
ApeKI GCWGC 5 cut(s) 740, 1655, 2012, 2373, 2624
ApoI RAATTY 3 cut(s) 253, 311, 1325
Asp700I GAANNNNTTC 1 cut(s) 253
Asp718I GGTACC 1 cut(s) 2109
AspLEI GCGC 3 cut(s) 1447, 1887, 2002
AspS9I GGNCC 4 cut(s) 498, 1121, 1122, 2542
AsuC2I CCSGG 1 cut(s) 2251
AsuHPI GGTGA 4 cut(s) 428, 982, 1370, 2239
AsuII TTCGAA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 2542
BaeGI GKGCMC 1 cut(s) 1125
BaeI ACNNNNGTAYC 2 cut(s) 94, 127
BamHI GGATCC 1 cut(s) 1540
BanI GGYRCC 1 cut(s) 2109
BanII GRGCYC 2 cut(s) 559, 1125
BbrPI CACGTG 2 cut(s) 122, 935
BbsI GAAGAC 2 cut(s) 2314, 2332
Bbv12I GWGCWC 2 cut(s) 2133, 2161
BbvI GCAGC 5 cut(s) 752, 1642, 2024, 2385, 2611
BceAI ACGGC 1 cut(s) 1315
BciT130I CCWGG 4 cut(s) 689, 1143, 1827, 2006
BciVI GTATCC 1 cut(s) 981
BclI TGATCA 1 cut(s) 2662
BcnI CCSGG 1 cut(s) 2251
BcoDI GTCTC 4 cut(s) 933, 1010, 1640, 1962
BcuI ACTAGT 1 cut(s) 1339
BfaI CTAG 5 cut(s) 378, 1340, 2075, 2289, 2604
BfmI CTRYAG 1 cut(s) 1068
BfoI RGCGCY 1 cut(s) 1888
BfuAI ACCTGC 1 cut(s) 1895
BfuI GTATCC 1 cut(s) 981
BglI GCCNNNNNGGC 1 cut(s) 1399
BglII AGATCT 1 cut(s) 961
BisI GCNGC 6 cut(s) 741, 1656, 2010, 2013, 2374, 2625
BlpI GCTNAGC 2 cut(s) 885, 2628
BlsI GCNGC 6 cut(s) 742, 1657, 2011, 2014, 2375, 2626
Bme1390I CCNGG 5 cut(s) 689, 1143, 1827, 2006, 2251
Bme18I GGWCC 1 cut(s) 2542
BmeRI GACNNNNNGTC 1 cut(s) 2181
BmgT120I GGNCC 4 cut(s) 498, 1121, 1122, 2542
BmiI GGNNCC 9 cut(s) 636, 1123, 1124, 1542, 2111, 2468, 2523, 2544, 2562
BmrFI CCNGG 5 cut(s) 689, 1143, 1827, 2006, 2251
BmrI ACTGGG 3 cut(s) 558, 1120, 2051
BmsI GCATC 7 cut(s) 723, 1456, 1513, 1646, 2074, 2208, 2545
BmuI ACTGGG 3 cut(s) 558, 1120, 2051
BpiI GAAGAC 2 cut(s) 2314, 2332
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 3 cut(s) 1515, 1901, 2334
Bpu10I CCTNAGC 1 cut(s) 1080
Bpu1102I GCTNAGC 2 cut(s) 885, 2628
Bpu14I TTCGAA 1 cut(s) 319
BpuMI CCSGG 1 cut(s) 2251
BsaAI YACGTR 2 cut(s) 122, 935
BsaBI GATNNNNATC 2 cut(s) 1503, 2487
BsaHI GRCGYC 1 cut(s) 1003
BsaI GGTCTC 2 cut(s) 933, 1640
BsaJI CCNNGG 5 cut(s) 638, 772, 1043, 1297, 1650
BsaWI WCCGGW 2 cut(s) 364, 2112
Bsc4I CCNNNNNNNGG 3 cut(s) 999, 1044, 2551
Bse118I RCCGGY 1 cut(s) 714
Bse3DI GCAATG 2 cut(s) 451, 750
Bse8I GATNNNNATC 2 cut(s) 1503, 2487
BseBI CCWGG 4 cut(s) 689, 1143, 1827, 2006
BseDI CCNNGG 5 cut(s) 638, 772, 1043, 1297, 1650
BseGI GGATG 8 cut(s) 24, 475, 565, 814, 983, 1594, 2280, 2560
BseJI GATNNNNATC 2 cut(s) 1503, 2487
BseLI CCNNNNNNNGG 3 cut(s) 999, 1044, 2551
BseMI GCAATG 2 cut(s) 451, 750
BseMII CTCAG 7 cut(s) 218, 899, 1071, 1347, 1799, 2112, 2642
BseRI GAGGAG 2 cut(s) 972, 2419
BseSI GKGCMC 1 cut(s) 1125
BseXI GCAGC 5 cut(s) 752, 1642, 2024, 2385, 2611
Bsh1236I CGCG 1 cut(s) 1445
BshFI GGCC 8 cut(s) 96, 139, 500, 513, 714, 1123, 2009, 2151
BshNI GGYRCC 1 cut(s) 2109
BsiHKAI GWGCWC 2 cut(s) 2133, 2161
BsiSI CCGG 4 cut(s) 365, 715, 2113, 2251
BslFI GGGAC 1 cut(s) 1928
BslI CCNNNNNNNGG 3 cut(s) 999, 1044, 2551
BsmAI GTCTC 4 cut(s) 933, 1010, 1640, 1962
BsmBI CGTCTC 1 cut(s) 1010
BsmFI GGGAC 1 cut(s) 1928
BsmI GAATGC 1 cut(s) 1867
BsnI GGCC 8 cut(s) 96, 139, 500, 513, 714, 1123, 2009, 2151
Bso31I GGTCTC 2 cut(s) 933, 1640
Bsp119I TTCGAA 1 cut(s) 319
Bsp120I GGGCCC 1 cut(s) 1121
Bsp1286I GDGCHC 4 cut(s) 559, 1125, 2133, 2161
Bsp1407I TGTACA 1 cut(s) 1872
Bsp1720I GCTNAGC 2 cut(s) 885, 2628
Bsp19I CCATGG 1 cut(s) 1043
BspACI CCGC 2 cut(s) 2010, 2558
BspANI GGCC 8 cut(s) 96, 139, 500, 513, 714, 1123, 2009, 2151
BspCNI CTCAG 7 cut(s) 219, 898, 1072, 1346, 1798, 2113, 2641
BspFNI CGCG 1 cut(s) 1445
BspHI TCATGA 1 cut(s) 1974
BspLI GGNNCC 9 cut(s) 636, 1123, 1124, 1542, 2111, 2468, 2523, 2544, 2562
BspMI ACCTGC 1 cut(s) 1895
BspPI GGATC 6 cut(s) 379, 786, 1535, 1548, 2135, 2496
BspQI GCTCTTC 1 cut(s) 1035
BspT104I TTCGAA 1 cut(s) 319
BspT107I GGYRCC 1 cut(s) 2109
BspTNI GGTCTC 2 cut(s) 933, 1640
BsrDI GCAATG 2 cut(s) 451, 750
BsrFI RCCGGY 1 cut(s) 714
BsrGI TGTACA 1 cut(s) 1872
BssAI RCCGGY 1 cut(s) 714
BssECI CCNNGG 5 cut(s) 638, 772, 1043, 1297, 1650
BssNAI GTATAC 2 cut(s) 1315, 2502
BssNI GRCGYC 1 cut(s) 1003
BssT1I CCWWGG 4 cut(s) 638, 772, 1043, 1650
Bst1107I GTATAC 2 cut(s) 1315, 2502
Bst2UI CCWGG 4 cut(s) 689, 1143, 1827, 2006
Bst4CI ACNGT 7 cut(s) 167, 203, 416, 676, 703, 1855, 2172
Bst6I CTCTTC 2 cut(s) 1035, 1229
BstACI GRCGYC 1 cut(s) 1003
BstAPI GCANNNNNTGC 1 cut(s) 2624
BstAUI TGTACA 1 cut(s) 1872
BstBAI YACGTR 2 cut(s) 122, 935
BstBI TTCGAA 1 cut(s) 319
BstC8I GCNNGC 5 cut(s) 141, 187, 442, 738, 2197
BstDEI CTNAG 8 cut(s) 176, 227, 885, 1080, 1333, 1785, 2121, 2628
BstDSI CCRYGG 2 cut(s) 1043, 1297
BstENI CCTNNNNNAGG 1 cut(s) 997
BstF5I GGATG 8 cut(s) 24, 475, 565, 814, 983, 1594, 2280, 2560
BstFNI CGCG 1 cut(s) 1445
BstH2I RGCGCY 1 cut(s) 1888
BstHHI GCGC 3 cut(s) 1447, 1887, 2002
BstMAI GTCTC 4 cut(s) 933, 1010, 1640, 1962
BstMWI GCNNNNNNNGC 7 cut(s) 186, 613, 733, 1399, 2006, 2569, 2624
BstNI CCWGG 4 cut(s) 689, 1143, 1827, 2006
BstNSI RCATGY 5 cut(s) 444, 727, 1607, 1879, 2199
BstSCI CCNGG 5 cut(s) 687, 1141, 1825, 2004, 2249
BstSFI CTRYAG 1 cut(s) 1068
BstSLI GKGCMC 1 cut(s) 1125
BstUI CGCG 1 cut(s) 1445
BstV1I GCAGC 5 cut(s) 752, 1642, 2024, 2385, 2611
BstV2I GAAGAC 2 cut(s) 2314, 2332
BstX2I RGATCY 5 cut(s) 371, 778, 961, 1540, 2488
BstYI RGATCY 5 cut(s) 371, 778, 961, 1540, 2488
BstZ17I GTATAC 2 cut(s) 1315, 2502
BsuI GTATCC 1 cut(s) 981
BsuRI GGCC 8 cut(s) 96, 139, 500, 513, 714, 1123, 2009, 2151
BtgI CCRYGG 2 cut(s) 1043, 1297
BtgZI GCGATG 1 cut(s) 858
BtsCI GGATG 8 cut(s) 24, 475, 565, 814, 983, 1594, 2280, 2560
BtsIMutI CAGTG 6 cut(s) 763, 1133, 1793, 2177, 2358, 2691
BveI ACCTGC 1 cut(s) 1895
Cac8I GCNNGC 5 cut(s) 141, 187, 442, 738, 2197
CaiI CAGNNNCTG 2 cut(s) 701, 1910
CciI TCATGA 1 cut(s) 1974
CfoI GCGC 3 cut(s) 1447, 1887, 2002
Cfr10I RCCGGY 1 cut(s) 714
Cfr13I GGNCC 4 cut(s) 498, 1121, 1122, 2542
CseI GACGC 2 cut(s) 992, 1505
Csp6I GTAC 6 cut(s) 103, 422, 920, 1414, 1873, 2110
CviQI GTAC 6 cut(s) 103, 422, 920, 1414, 1873, 2110
DdeI CTNAG 8 cut(s) 176, 227, 885, 1080, 1333, 1785, 2121, 2628
DraI TTTAAA 2 cut(s) 1324, 1707
DriI GACNNNNNGTC 1 cut(s) 2181
EaeI YGGCCR 2 cut(s) 712, 2007
Eam1104I CTCTTC 2 cut(s) 1035, 1229
Eam1105I GACNNNNNGTC 1 cut(s) 2181
EarI CTCTTC 2 cut(s) 1035, 1229
Eco130I CCWWGG 4 cut(s) 638, 772, 1043, 1650
Eco147I AGGCCT 2 cut(s) 513, 2151
Eco24I GRGCYC 2 cut(s) 559, 1125
Eco31I GGTCTC 2 cut(s) 933, 1640
Eco47I GGWCC 1 cut(s) 2542
Eco47III AGCGCT 1 cut(s) 1886
Eco57I CTGAAG 3 cut(s) 1016, 1127, 2067
Eco72I CACGTG 2 cut(s) 122, 935
EcoNI CCTNNNNNAGG 1 cut(s) 997
EcoO109I RGGNCCY 1 cut(s) 1122
EcoRII CCWGG 4 cut(s) 687, 1141, 1825, 2004
EcoT14I CCWWGG 4 cut(s) 638, 772, 1043, 1650
EcoT22I ATGCAT 1 cut(s) 2201
EcoT38I GRGCYC 2 cut(s) 559, 1125
ErhI CCWWGG 4 cut(s) 638, 772, 1043, 1650
Esp3I CGTCTC 1 cut(s) 1010
FalI AAGNNNNNCTT 6 cut(s) 241, 273, 765, 797, 1829, 1861
FaqI GGGAC 1 cut(s) 1928
FauNDI CATATG 1 cut(s) 1454
FbaI TGATCA 1 cut(s) 2662
FblI GTMKAC 2 cut(s) 1314, 2501
Fnu4HI GCNGC 6 cut(s) 741, 1656, 2010, 2013, 2374, 2625
FokI GGATG 8 cut(s) 31, 482, 552, 821, 970, 1581, 2287, 2567
FriOI GRGCYC 2 cut(s) 559, 1125
Fsp4HI GCNGC 6 cut(s) 741, 1656, 2010, 2013, 2374, 2625
FspBI CTAG 5 cut(s) 378, 1340, 2075, 2289, 2604
FspI TGCGCA 1 cut(s) 2001
GlaI GCGC 3 cut(s) 1446, 1886, 2001
GluI GCNGC 6 cut(s) 741, 1656, 2010, 2013, 2374, 2625
GsuI CTGGAG 3 cut(s) 1515, 1901, 2334
HaeII RGCGCY 1 cut(s) 1888
HaeIII GGCC 8 cut(s) 96, 139, 500, 513, 714, 1123, 2009, 2151
HapII CCGG 4 cut(s) 365, 715, 2113, 2251
HgaI GACGC 2 cut(s) 992, 1505
HhaI GCGC 3 cut(s) 1447, 1887, 2002
Hin1I GRCGYC 1 cut(s) 1003
Hin6I GCGC 3 cut(s) 1445, 1885, 2000
HinP1I GCGC 3 cut(s) 1445, 1885, 2000
HincII GTYRAC 1 cut(s) 546
HindII GTYRAC 1 cut(s) 546
HinfI GANTC 8 cut(s) 6, 22, 914, 1367, 1676, 1817, 2020, 2279
HpaII CCGG 4 cut(s) 365, 715, 2113, 2251
HphI GGTGA 4 cut(s) 428, 982, 1370, 2239
Hpy166II GTNNAC 5 cut(s) 546, 1315, 1873, 2175, 2502
Hpy188I TCNGA 7 cut(s) 1102, 1251, 1336, 1716, 1962, 2019, 2526
Hpy8I GTNNAC 5 cut(s) 546, 1315, 1873, 2175, 2502
Hpy99I CGWCG 2 cut(s) 862, 1951
HpyAV CCTTC 8 cut(s) 126, 326, 799, 900, 997, 1467, 1674, 2162
HpyCH4III ACNGT 7 cut(s) 167, 203, 416, 676, 703, 1855, 2172
HpyCH4IV ACGT 3 cut(s) 121, 934, 2177
HpyF10VI GCNNNNNNNGC 7 cut(s) 186, 613, 733, 1399, 2006, 2569, 2624
HpyF3I CTNAG 8 cut(s) 176, 227, 885, 1080, 1333, 1785, 2121, 2628
HpySE526I ACGT 3 cut(s) 121, 934, 2177
Hsp92I GRCGYC 1 cut(s) 1003
HspAI GCGC 3 cut(s) 1445, 1885, 2000
KpnI GGTACC 1 cut(s) 2113
Ksp22I TGATCA 1 cut(s) 2662
LguI GCTCTTC 1 cut(s) 1035
LmnI GCTCC 5 cut(s) 367, 764, 1634, 1882, 2560
Lsp1109I GCAGC 5 cut(s) 752, 1642, 2024, 2385, 2611
LweI GCATC 7 cut(s) 723, 1456, 1513, 1646, 2074, 2208, 2545
MaeI CTAG 5 cut(s) 378, 1340, 2075, 2289, 2604
MaeII ACGT 3 cut(s) 121, 934, 2177
MaeIII GTNAC 6 cut(s) 49, 117, 697, 851, 1829, 2245
MflI RGATCY 5 cut(s) 371, 778, 961, 1540, 2488
MhlI GDGCHC 4 cut(s) 559, 1125, 2133, 2161
MlyI GAGTC 3 cut(s) 15, 31, 1811
MmeI TCCRAC 3 cut(s) 447, 2016, 2168
Mph1103I ATGCAT 1 cut(s) 2201
MroXI GAANNNNTTC 1 cut(s) 253
MslI CAYNNNNRTG 1 cut(s) 728
MspA1I CMGCKG 2 cut(s) 659, 2120
MspI CCGG 4 cut(s) 365, 715, 2113, 2251
MspR9I CCNGG 5 cut(s) 689, 1143, 1827, 2006, 2251
Mva1269I GAATGC 1 cut(s) 1867
MvaI CCWGG 4 cut(s) 689, 1143, 1827, 2006
MvnI CGCG 1 cut(s) 1445
MwoI GCNNNNNNNGC 7 cut(s) 186, 613, 733, 1399, 2006, 2569, 2624
NciI CCSGG 1 cut(s) 2251
NcoI CCATGG 1 cut(s) 1043
NdeI CATATG 1 cut(s) 1454
NlaIV GGNNCC 9 cut(s) 636, 1123, 1124, 1542, 2111, 2468, 2523, 2544, 2562
NmeAIII GCCGAG 1 cut(s) 853
NmuCI GTSAC 4 cut(s) 49, 117, 1829, 2245
NsbI TGCGCA 1 cut(s) 2001
NsiI ATGCAT 1 cut(s) 2201
NspI RCATGY 5 cut(s) 444, 727, 1607, 1879, 2199
NspV TTCGAA 1 cut(s) 319
PaeI GCATGC 2 cut(s) 444, 2199
PagI TCATGA 1 cut(s) 1974
PaqCI CACCTGC 1 cut(s) 1895
PceI AGGCCT 2 cut(s) 513, 2151
PciI ACATGT 1 cut(s) 1603
PciSI GCTCTTC 1 cut(s) 1035
PctI GAATGC 1 cut(s) 1867
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 5 cut(s) 914, 1367, 1676, 2020, 2279
PfoI TCCNGGA 1 cut(s) 1141
PkrI GCNGC 6 cut(s) 742, 1657, 2011, 2014, 2375, 2626
PleI GAGTC 3 cut(s) 14, 30, 1811
PmaCI CACGTG 2 cut(s) 122, 935
PmlI CACGTG 2 cut(s) 122, 935
PpsI GAGTC 3 cut(s) 14, 30, 1811
Ppu21I YACGTR 2 cut(s) 122, 935
PscI ACATGT 1 cut(s) 1603
Psp6I CCWGG 4 cut(s) 687, 1141, 1825, 2004
PspCI CACGTG 2 cut(s) 122, 935
PspGI CCWGG 4 cut(s) 687, 1141, 1825, 2004
PspN4I GGNNCC 9 cut(s) 636, 1123, 1124, 1542, 2111, 2468, 2523, 2544, 2562
PspOMI GGGCCC 1 cut(s) 1121
PspPI GGNCC 4 cut(s) 498, 1121, 1122, 2542
PstNI CAGNNNCTG 2 cut(s) 701, 1910
PsuI RGATCY 5 cut(s) 371, 778, 961, 1540, 2488
PvuII CAGCTG 2 cut(s) 659, 2120
RsaI GTAC 6 cut(s) 104, 423, 921, 1415, 1874, 2111
RsaNI GTAC 6 cut(s) 103, 422, 920, 1414, 1873, 2110
RseI CAYNNNNRTG 1 cut(s) 728
SapI GCTCTTC 1 cut(s) 1035
SatI GCNGC 6 cut(s) 741, 1656, 2010, 2013, 2374, 2625
Sau96I GGNCC 4 cut(s) 498, 1121, 1122, 2542
SchI GAGTC 3 cut(s) 15, 31, 1811
ScrFI CCNGG 5 cut(s) 689, 1143, 1827, 2006, 2251
SduI GDGCHC 4 cut(s) 559, 1125, 2133, 2161
SfaNI GCATC 7 cut(s) 723, 1456, 1513, 1646, 2074, 2208, 2545
SfcI CTRYAG 1 cut(s) 1068
SfuI TTCGAA 1 cut(s) 319
SinI GGWCC 1 cut(s) 2542
SmiI ATTTAAAT 1 cut(s) 1324
SmiMI CAYNNNNRTG 1 cut(s) 728
SpeI ACTAGT 1 cut(s) 1339
SphI GCATGC 2 cut(s) 444, 2199
SseBI AGGCCT 2 cut(s) 513, 2151
SsiI CCGC 2 cut(s) 2010, 2558
SspI AATATT 1 cut(s) 1702
SspMI CTAG 5 cut(s) 378, 1340, 2075, 2289, 2604
StuI AGGCCT 2 cut(s) 513, 2151
StyD4I CCNGG 5 cut(s) 687, 1141, 1825, 2004, 2249
StyI CCWWGG 4 cut(s) 638, 772, 1043, 1650
SwaI ATTTAAAT 1 cut(s) 1324
TaaI ACNGT 7 cut(s) 167, 203, 416, 676, 703, 1855, 2172
TaiI ACGT 3 cut(s) 124, 937, 2180
TaqI TCGA 6 cut(s) 9, 319, 1161, 1797, 1949, 2677
TaqII GACCGA 2 cut(s) 98, 2171
TatI WGTACW 2 cut(s) 919, 1872
TauI GCSGC 1 cut(s) 2012
TfiI GAWTC 5 cut(s) 914, 1367, 1676, 2020, 2279
TscAI CASTG 6 cut(s) 763, 1133, 1793, 2177, 2358, 2698
TseFI GTSAC 4 cut(s) 49, 117, 1829, 2245
TseI GCWGC 5 cut(s) 740, 1655, 2012, 2373, 2624
Tsp45I GTSAC 4 cut(s) 49, 117, 1829, 2245
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 6 cut(s) 763, 1133, 1793, 2177, 2358, 2698
VpaK11BI GGWCC 1 cut(s) 2542
XagI CCTNNNNNAGG 1 cut(s) 997
XapI RAATTY 3 cut(s) 253, 311, 1325
XbaI TCTAGA 1 cut(s) 2288
XceI RCATGY 5 cut(s) 444, 727, 1607, 1879, 2199
XmiI GTMKAC 2 cut(s) 1314, 2501
XmnI GAANNNNTTC 1 cut(s) 253
XspI CTAG 5 cut(s) 378, 1340, 2075, 2289, 2604
Zsp2I ATGCAT 1 cut(s) 2201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.