Rh6DG469800

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005367
Physical Location & Seq
Forward (+)
63693150 .. 63701166
8017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG469800.1

Sequence Viewer

Length: 2721 bp
ATGGGAGTTGATATACGGATGAGTTTGTCTTTGGGTTTTAATGTATTTGTCACTTCTGTCCTGTTTCTTCTTTTCTTCCACATTGTATTTATTGGCCTTTGGTATATCGGTCTCGTGTCACGTGTAGCTGGAAGGAGGCCAGCAATTTTAACAATCCTCCAAAACTGTGCTGTTCTTAGCGTAGCCTGCTGTATATTTTACAGTCATTGTGGCAACCGAGCTATCCTGAGAGAGAGACAACTGGATAGAAGAAATTCTTTGTCTTGGTTTAATTTCTGGAAGAAAGATGAGAGAAATACGTGGCTTTCTAAATTCCTTCGAATGAATGAGCTGAAAGATGAGGTCTGCTCATACTGGTTTGCTCCGGTAGGGTCTGCAAGTGATTATCCACTTTTGTCCAAGTGGGTCATCTATGGTGAGGTACAGTTAGCATGCAATGGTTCATGTGCTGGTTCATCGGATGGAATATCCCCATTATACTCATTATGGGCCACATTCATAGGCCTGTATATTGCTAATTATGTAGTGGAGAGGTCAACAGGGTGGGCTCTTACTCATCCTTTGTCTGTAGAAGAACATGAGAAGTCGAAGAACAAGCAAATGAAGCCTGATTTTTTGGATATGGTTCCTTGGTATTCAGGAACATCAGCTGATTTGTTCAAGACTGTTTTTGACCTCCTGGTATCAGTAACTGTCTTTGTTGGCCGGTTTGACATGCGTATGATGCAGGCAGCAATGAGCAAGATCAGTGACGGAGCTTCACAAGGGGATCTTTTATATGATGATTTTGTTGAGAAGGATGATCTATGGTTTGACTTCATGGCTGATACTGGCGATGGTGGAAACTCATCTTATTCTGTGGCTCGGCTCCTCGCTCAGCCTTCCATCAATGTCAGCAAAGACGATTCTGTACTTAACCTACCACGTGGAGACCTACTACTTATTGGAGGAGATCTTGCGTATCCTAATCCTTCAGCCTTCACCTATGAAAGGCGTCTCTTTTGTCCTTTCGAGTATGCTCTCCAGCCCCCTTCATGGTCTAAACAAGAGCATATAGCTGTAGATAAACCTGAGCTACCTTTTGGCGTGTCTGAACTAAAGCAATATGATGGGCCCCAGTGTTTTGTCATTCCTGGAAACCATGATTGGTTTGATGGACTTAATACATTTATGAGGTATATATGTCATAAGAGCTGGTTGGGTGGGTGGCTTATGCCTCAAAAGAAGAGTTATTTTGCTCTGAAACTCCCTAAAAGATGGTGGGTGTTTGGTCTTGATCTGGCACTCCACGGCGATATTGATGTGTACCAATTCAAATTCTTTTCAGAACTAGTAAAGAACAAGGTAGGAGAAGATGATTCTGTAATCATTATGACACATGAGCCAAACTGGCTTCTTGACTGGTACTGGAATGATGTATCTGGAAAGAATGTTGCACATCTGATATGCGATCATTTGAAAGGAAGGTGTAAGCTACGAGTGGCTGGAGATTTGCATCATTATATGCGTCATTCATTTGTTAGGTCAGAGGACCCAGTTCAAGTGCAACATTTACTTGTAAACGGTTGTGGTGGGGCTTTTCTACATCCCACACATGTGTTCAGTAATTTTAAGAAATTTTATGGAGCATCGTACGAGACCAAGGCTGCTTATCCTTCATTTGAAGATTCAAGTAGGATTGCTTTAGGAAATATTTTAAAGTTTCGGAAGAAGAACTGGCAATTTGATTTTATTGGTGGCATTATATACTTTCTATTGGTCTTTTCTATGTTCCCACAGTGTAAGCTCAATCATATCTTGCGAGAGGACTCATTTCCTGGTCACTTGAGGTCTTTCTTTGGAACAGTGTGGAATGCTTTTGTGTACATGCTGGAGCAGTCTTATGTATCGTTAGCAGGTGCTGTGGTGTTGCTGATTGTGGCAGTCACATTTGTCCCCTCCAAAGTGTCTCGTAAGAAACGGGTTATGATTGGAGTTCTTCATGTGTCTGCGCACCTGGCTGCAGCTCTAATTCTTATGTTGCTGTTGGAACTGGGTGTTGAGATGTGCGTCCAGCATCAACTACTAGGAACTTCAGGCTATCACACATTATATGAGTGGTACCGGTCAGCGGAGAGTGAGCACTTTCCAGATCCAACTGGCCTTCGTGCTCGTATAGAACAATGGACCTTGGGTCTTTATCCAGCATGCATCAAGTATTTCATGTCTGCATTTGATGTTCCAGAGGTTATGGCAGTCACCCGGAACAATATATGCAAGAATGGGATGGAATCACTCTCTAGACTGGGTGCTTGTATATATTATGCTTCTGTCTTCCTTTATTTTTGGGTCTTCTCTACTCCAGTGGTTTCTTTGGTGTTTGGCAGCTACCTATATGTTTGCATTAATTGGTTGCACATACACTTTGATGAAGCGTTCTCCTCCTTGCGAATTGCTAATTACAAATCATTCACTCGATTTCACATTAATACTGATGGGGATCTTGAAGTGTATACTCTTGCAGTCGATAAGGTTCCAAAAGAATGGAAGCTGGACCCTGATTGGGATGCGGAGCCAAAACAACCGCAACAGATGAGCCATCGGAGAAAGTTTCCTAGTAAATGGAGTGCAAAAGCTGCTCAGCAAGACCCACTACATAATGTTAAAATAGTTGATCACTTTGTAATTCGACAAACAGATAAAACTGACGTAGCAGGAAGTGATGGTTCAGTAATTCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

906

Amino Acids

103.68

Weight (kDa)

7.35

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 273 - 473 5.5e-07 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1886
Acc16I TGCGCA 1 cut(s) 1992
Acc36I ACCTGC 1 cut(s) 1886
Acc65I GGTACC 1 cut(s) 2100
AccB1I GGYRCC 1 cut(s) 2100
AccI GTMKAC 1 cut(s) 2492
AciI CCGC 3 cut(s) 2111, 2549, 2564
AclWI GGATC 3 cut(s) 777, 2126, 2487
AcoI YGGCCR 1 cut(s) 703
AcsI RAATTY 4 cut(s) 253, 311, 1316, 1616
AcuI CTGAAG 2 cut(s) 957, 2058
AcvI CACGTG 2 cut(s) 122, 926
AcyI GRCGYC 1 cut(s) 994
AfaI GTAC 7 cut(s) 423, 912, 1307, 1406, 1634, 1865, 2102
AfiI CCNNNNNNNGG 4 cut(s) 990, 1035, 2110, 2542
AflIII ACRYGT 2 cut(s) 121, 1594
AgeI ACCGGT 1 cut(s) 2103
AgsI TTSAA 7 cut(s) 661, 1315, 1459, 1541, 1664, 1671, 2486
AhdI GACNNNNNGTC 1 cut(s) 2172
AhlI ACTAGT 1 cut(s) 1330
AjnI CCWGG 4 cut(s) 678, 1132, 1816, 1995
AjuI GAANNNNNNNTTGG 2 cut(s) 1129, 1161
AleI CACNNNNGTG 1 cut(s) 1595
Alw21I GWGCWC 2 cut(s) 2124, 2152
Alw26I GTCTC 6 cut(s) 116, 229, 924, 1001, 1631, 1953
AlwI GGATC 3 cut(s) 777, 2126, 2487
AlwNI CAGNNNCTG 2 cut(s) 692, 1901
AoxI GGCC 7 cut(s) 94, 137, 489, 502, 703, 1112, 2140
ApaI GGGCCC 1 cut(s) 1116
ApeKI GCWGC 6 cut(s) 731, 1646, 2000, 2003, 2364, 2615
ApoI RAATTY 4 cut(s) 253, 311, 1316, 1616
AseI ATTAAT 2 cut(s) 2385, 2466
AsiGI ACCGGT 1 cut(s) 2103
Asp700I GAANNNNTTC 1 cut(s) 253
Asp718I GGTACC 1 cut(s) 2100
AspLEI GCGC 1 cut(s) 1993
AspS9I GGNCC 6 cut(s) 489, 1112, 1113, 1531, 2166, 2533
AsuC2I CCSGG 1 cut(s) 2242
AsuHPI GGTGA 3 cut(s) 428, 973, 2230
AsuII TTCGAA 1 cut(s) 319
AvaII GGWCC 3 cut(s) 1531, 2166, 2533
BaeGI GKGCMC 1 cut(s) 1116
BanI GGYRCC 1 cut(s) 2100
BanII GRGCYC 2 cut(s) 550, 1116
BauI CACGAG 1 cut(s) 113
BbrPI CACGTG 2 cut(s) 122, 926
BbsI GAAGAC 2 cut(s) 2305, 2323
Bbv12I GWGCWC 2 cut(s) 2124, 2152
BbvI GCAGC 6 cut(s) 743, 1633, 1987, 2015, 2376, 2602
BceAI ACGGC 1 cut(s) 1306
BciT130I CCWGG 4 cut(s) 680, 1134, 1818, 1997
BciVI GTATCC 1 cut(s) 972
BclI TGATCA 1 cut(s) 2653
BcnI CCSGG 1 cut(s) 2242
BcoDI GTCTC 6 cut(s) 116, 229, 924, 1001, 1631, 1953
BcuI ACTAGT 1 cut(s) 1330
BfaI CTAG 4 cut(s) 1331, 2066, 2280, 2595
BfmI CTRYAG 3 cut(s) 567, 1059, 2001
BfuAI ACCTGC 1 cut(s) 1886
BfuI GTATCC 1 cut(s) 972
BglI GCCNNNNNGGC 1 cut(s) 1390
BglII AGATCT 1 cut(s) 952
BisI GCNGC 6 cut(s) 732, 1647, 2001, 2004, 2365, 2616
BlpI GCTNAGC 2 cut(s) 876, 2619
BlsI GCNGC 6 cut(s) 733, 1648, 2002, 2005, 2366, 2617
Bme1390I CCNGG 5 cut(s) 680, 1134, 1818, 1997, 2242
Bme18I GGWCC 3 cut(s) 1531, 2166, 2533
BmeRI GACNNNNNGTC 1 cut(s) 2172
BmgT120I GGNCC 6 cut(s) 489, 1112, 1113, 1531, 2166, 2533
BmiI GGNNCC 9 cut(s) 627, 869, 1114, 1115, 1533, 2102, 2514, 2535, 2553
BmrFI CCNGG 5 cut(s) 680, 1134, 1818, 1997, 2242
BmrI ACTGGG 4 cut(s) 1111, 1529, 2042, 2294
BmsI GCATC 6 cut(s) 714, 1504, 1637, 2065, 2199, 2536
BmuI ACTGGG 4 cut(s) 1111, 1529, 2042, 2294
BpiI GAAGAC 2 cut(s) 2305, 2323
BplI GAGNNNNNCTC 2 cut(s) 332, 364
BpmI CTGGAG 4 cut(s) 1007, 1506, 1892, 2325
Bpu10I CCTNAGC 1 cut(s) 1071
Bpu1102I GCTNAGC 2 cut(s) 876, 2619
Bpu14I TTCGAA 1 cut(s) 319
BpuEI CTTGAG 1 cut(s) 1846
BpuMI CCSGG 1 cut(s) 2242
BsaAI YACGTR 3 cut(s) 122, 300, 926
BsaBI GATNNNNATC 2 cut(s) 1494, 2478
BsaHI GRCGYC 1 cut(s) 994
BsaI GGTCTC 3 cut(s) 116, 924, 1631
BsaJI CCNNGG 4 cut(s) 629, 1288, 1641, 2169
BsaWI WCCGGW 2 cut(s) 364, 2103
Bsc4I CCNNNNNNNGG 4 cut(s) 990, 1035, 2110, 2542
Bse118I RCCGGY 2 cut(s) 705, 2103
Bse3DI GCAATG 2 cut(s) 442, 741
Bse8I GATNNNNATC 2 cut(s) 1494, 2478
BseBI CCWGG 4 cut(s) 680, 1134, 1818, 1997
BseDI CCNNGG 4 cut(s) 629, 1288, 1641, 2169
BseGI GGATG 7 cut(s) 24, 466, 556, 805, 1585, 2271, 2551
BseJI GATNNNNATC 2 cut(s) 1494, 2478
BseLI CCNNNNNNNGG 4 cut(s) 990, 1035, 2110, 2542
BseMI GCAATG 2 cut(s) 442, 741
BseMII CTCAG 4 cut(s) 218, 890, 1062, 2633
BseRI GAGGAG 3 cut(s) 860, 963, 2410
BseSI GKGCMC 1 cut(s) 1116
BseXI GCAGC 6 cut(s) 743, 1633, 1987, 2015, 2376, 2602
BshFI GGCC 7 cut(s) 96, 139, 491, 504, 705, 1114, 2142
BshNI GGYRCC 1 cut(s) 2100
BshTI ACCGGT 1 cut(s) 2103
BsiHKAI GWGCWC 2 cut(s) 2124, 2152
BsiSI CCGG 4 cut(s) 365, 706, 2104, 2242
BsiWI CGTACG 1 cut(s) 1632
BslFI GGGAC 1 cut(s) 1919
BslI CCNNNNNNNGG 4 cut(s) 990, 1035, 2110, 2542
BsmAI GTCTC 6 cut(s) 116, 229, 924, 1001, 1631, 1953
BsmBI CGTCTC 1 cut(s) 1001
BsmFI GGGAC 1 cut(s) 1919
BsmI GAATGC 1 cut(s) 1858
BsnI GGCC 7 cut(s) 96, 139, 491, 504, 705, 1114, 2142
Bso31I GGTCTC 3 cut(s) 116, 924, 1631
Bsp119I TTCGAA 1 cut(s) 319
Bsp120I GGGCCC 1 cut(s) 1112
Bsp1286I GDGCHC 4 cut(s) 550, 1116, 2124, 2152
Bsp1407I TGTACA 1 cut(s) 1863
Bsp143I GATC 9 cut(s) 744, 769, 802, 952, 1276, 1450, 2131, 2479, 2653
Bsp1720I GCTNAGC 2 cut(s) 876, 2619
BspACI CCGC 3 cut(s) 2111, 2549, 2564
BspANI GGCC 7 cut(s) 96, 139, 491, 504, 705, 1114, 2142
BspCNI CTCAG 4 cut(s) 219, 889, 1063, 2632
BspLI GGNNCC 9 cut(s) 627, 869, 1114, 1115, 1533, 2102, 2514, 2535, 2553
BspMAI CTGCAG 1 cut(s) 2005
BspMI ACCTGC 1 cut(s) 1886
BspPI GGATC 3 cut(s) 777, 2126, 2487
BspT104I TTCGAA 1 cut(s) 319
BspT107I GGYRCC 1 cut(s) 2100
BspTNI GGTCTC 3 cut(s) 116, 924, 1631
BsrDI GCAATG 2 cut(s) 442, 741
BsrFI RCCGGY 2 cut(s) 705, 2103
BsrGI TGTACA 1 cut(s) 1863
BssAI RCCGGY 2 cut(s) 705, 2103
BssECI CCNNGG 4 cut(s) 629, 1288, 1641, 2169
BssMI GATC 9 cut(s) 744, 769, 802, 952, 1276, 1450, 2131, 2479, 2653
BssNAI GTATAC 1 cut(s) 2493
BssNI GRCGYC 1 cut(s) 994
BssSI CACGAG 1 cut(s) 113
BssT1I CCWWGG 3 cut(s) 629, 1641, 2169
Bst1107I GTATAC 1 cut(s) 2493
Bst2BI CACGAG 1 cut(s) 113
Bst2UI CCWGG 4 cut(s) 680, 1134, 1818, 1997
Bst4CI ACNGT 8 cut(s) 167, 203, 426, 667, 694, 1565, 1779, 1846
Bst6I CTCTTC 1 cut(s) 1220
BstACI GRCGYC 1 cut(s) 994
BstAPI GCANNNNNTGC 1 cut(s) 2615
BstAUI TGTACA 1 cut(s) 1863
BstBAI YACGTR 3 cut(s) 122, 300, 926
BstBI TTCGAA 1 cut(s) 319
BstC8I GCNNGC 5 cut(s) 141, 187, 433, 729, 2188
BstDEI CTNAG 5 cut(s) 176, 227, 876, 1071, 2619
BstDSI CCRYGG 1 cut(s) 1288
BstENI CCTNNNNNAGG 1 cut(s) 988
BstF5I GGATG 7 cut(s) 24, 466, 556, 805, 1585, 2271, 2551
BstHHI GCGC 1 cut(s) 1993
BstKTI GATC 9 cut(s) 747, 772, 805, 955, 1279, 1453, 2134, 2482, 2656
BstMAI GTCTC 6 cut(s) 116, 229, 924, 1001, 1631, 1953
BstMBI GATC 9 cut(s) 744, 769, 802, 952, 1276, 1450, 2131, 2479, 2653
BstMWI GCNNNNNNNGC 6 cut(s) 186, 604, 724, 1390, 1997, 2615
BstNI CCWGG 4 cut(s) 680, 1134, 1818, 1997
BstNSI RCATGY 5 cut(s) 435, 718, 1598, 1870, 2190
BstSCI CCNGG 5 cut(s) 678, 1132, 1816, 1995, 2240
BstSFI CTRYAG 3 cut(s) 567, 1059, 2001
BstSLI GKGCMC 1 cut(s) 1116
BstV1I GCAGC 6 cut(s) 743, 1633, 1987, 2015, 2376, 2602
BstV2I GAAGAC 2 cut(s) 2305, 2323
BstX2I RGATCY 4 cut(s) 769, 952, 2131, 2479
BstXI CCANNNNNNTGG 1 cut(s) 2523
BstYI RGATCY 4 cut(s) 769, 952, 2131, 2479
BstZ17I GTATAC 1 cut(s) 2493
BsuI GTATCC 1 cut(s) 972
BsuRI GGCC 7 cut(s) 96, 139, 491, 504, 705, 1114, 2142
BtgI CCRYGG 1 cut(s) 1288
BtgZI GCGATG 1 cut(s) 849
BtsCI GGATG 7 cut(s) 24, 466, 556, 805, 1585, 2271, 2551
BtsIMutI CAGTG 5 cut(s) 754, 1124, 1784, 1851, 2349
BveI ACCTGC 1 cut(s) 1886
Cac8I GCNNGC 5 cut(s) 141, 187, 433, 729, 2188
CaiI CAGNNNCTG 2 cut(s) 692, 1901
CfoI GCGC 1 cut(s) 1993
Cfr10I RCCGGY 2 cut(s) 705, 2103
Cfr13I GGNCC 6 cut(s) 489, 1112, 1113, 1531, 2166, 2533
CseI GACGC 3 cut(s) 983, 1496, 2038
Csp6I GTAC 7 cut(s) 422, 911, 1306, 1405, 1633, 1864, 2101
CspAI ACCGGT 1 cut(s) 2103
CviQI GTAC 7 cut(s) 422, 911, 1306, 1405, 1633, 1864, 2101
DdeI CTNAG 5 cut(s) 176, 227, 876, 1071, 2619
DpnI GATC 9 cut(s) 746, 771, 804, 954, 1278, 1452, 2133, 2481, 2655
DpnII GATC 9 cut(s) 744, 769, 802, 952, 1276, 1450, 2131, 2479, 2653
DraI TTTAAA 1 cut(s) 1698
DriI GACNNNNNGTC 1 cut(s) 2172
EaeI YGGCCR 1 cut(s) 703
Eam1104I CTCTTC 1 cut(s) 1220
Eam1105I GACNNNNNGTC 1 cut(s) 2172
EarI CTCTTC 1 cut(s) 1220
Eco130I CCWWGG 3 cut(s) 629, 1641, 2169
Eco147I AGGCCT 1 cut(s) 504
Eco24I GRGCYC 2 cut(s) 550, 1116
Eco31I GGTCTC 3 cut(s) 116, 924, 1631
Eco47I GGWCC 3 cut(s) 1531, 2166, 2533
Eco57I CTGAAG 2 cut(s) 957, 2058
Eco72I CACGTG 2 cut(s) 122, 926
EcoNI CCTNNNNNAGG 1 cut(s) 988
EcoO109I RGGNCCY 2 cut(s) 1113, 1531
EcoRII CCWGG 4 cut(s) 678, 1132, 1816, 1995
EcoT14I CCWWGG 3 cut(s) 629, 1641, 2169
EcoT22I ATGCAT 1 cut(s) 2192
EcoT38I GRGCYC 2 cut(s) 550, 1116
ErhI CCWWGG 3 cut(s) 629, 1641, 2169
Esp3I CGTCTC 1 cut(s) 1001
FalI AAGNNNNNCTT 4 cut(s) 241, 273, 756, 788
FaqI GGGAC 1 cut(s) 1919
FbaI TGATCA 1 cut(s) 2653
FblI GTMKAC 1 cut(s) 2492
Fnu4HI GCNGC 6 cut(s) 732, 1647, 2001, 2004, 2365, 2616
FokI GGATG 7 cut(s) 31, 473, 543, 812, 1572, 2278, 2558
FriOI GRGCYC 2 cut(s) 550, 1116
Fsp4HI GCNGC 6 cut(s) 732, 1647, 2001, 2004, 2365, 2616
FspBI CTAG 4 cut(s) 1331, 2066, 2280, 2595
FspI TGCGCA 1 cut(s) 1992
GlaI GCGC 1 cut(s) 1992
GluI GCNGC 6 cut(s) 732, 1647, 2001, 2004, 2365, 2616
GsuI CTGGAG 4 cut(s) 1007, 1506, 1892, 2325
HaeIII GGCC 7 cut(s) 96, 139, 491, 504, 705, 1114, 2142
HapII CCGG 4 cut(s) 365, 706, 2104, 2242
HgaI GACGC 3 cut(s) 983, 1496, 2038
HhaI GCGC 1 cut(s) 1993
Hin1I GRCGYC 1 cut(s) 994
Hin6I GCGC 1 cut(s) 1991
HinP1I GCGC 1 cut(s) 1991
HincII GTYRAC 1 cut(s) 537
HindII GTYRAC 1 cut(s) 537
HinfI GANTC 5 cut(s) 905, 1358, 1667, 1808, 2270
HpaII CCGG 4 cut(s) 365, 706, 2104, 2242
HphI GGTGA 3 cut(s) 428, 973, 2230
Hpy166II GTNNAC 5 cut(s) 537, 1306, 1561, 1864, 2493
Hpy188I TCNGA 8 cut(s) 460, 1093, 1242, 1327, 1443, 1528, 1707, 2583
Hpy8I GTNNAC 5 cut(s) 537, 1306, 1561, 1864, 2493
HpyCH4III ACNGT 8 cut(s) 167, 203, 426, 667, 694, 1565, 1779, 1846
HpyCH4IV ACGT 4 cut(s) 121, 299, 925, 2688
HpyF10VI GCNNNNNNNGC 6 cut(s) 186, 604, 724, 1390, 1997, 2615
HpyF3I CTNAG 5 cut(s) 176, 227, 876, 1071, 2619
HpySE526I ACGT 4 cut(s) 121, 299, 925, 2688
Hsp92I GRCGYC 1 cut(s) 994
HspAI GCGC 1 cut(s) 1991
KpnI GGTACC 1 cut(s) 2104
Ksp22I TGATCA 1 cut(s) 2653
Kzo9I GATC 9 cut(s) 744, 769, 802, 952, 1276, 1450, 2131, 2479, 2653
LmnI GCTCC 6 cut(s) 367, 755, 873, 1625, 1873, 2551
Lsp1109I GCAGC 6 cut(s) 743, 1633, 1987, 2015, 2376, 2602
LweI GCATC 6 cut(s) 714, 1504, 1637, 2065, 2199, 2536
MaeI CTAG 4 cut(s) 1331, 2066, 2280, 2595
MaeII ACGT 4 cut(s) 121, 299, 925, 2688
MaeIII GTNAC 7 cut(s) 49, 117, 688, 749, 1820, 1924, 2236
MalI GATC 9 cut(s) 746, 771, 804, 954, 1278, 1452, 2133, 2481, 2655
MboI GATC 9 cut(s) 744, 769, 802, 952, 1276, 1450, 2131, 2479, 2653
MflI RGATCY 4 cut(s) 769, 952, 2131, 2479
MhlI GDGCHC 4 cut(s) 550, 1116, 2124, 2152
MlyI GAGTC 1 cut(s) 1802
MmeI TCCRAC 2 cut(s) 2007, 2159
Mph1103I ATGCAT 1 cut(s) 2192
MroXI GAANNNNTTC 1 cut(s) 253
MslI CAYNNNNRTG 3 cut(s) 719, 1595, 2406
MspA1I CMGCKG 2 cut(s) 650, 2111
MspI CCGG 4 cut(s) 365, 706, 2104, 2242
MspR9I CCNGG 5 cut(s) 680, 1134, 1818, 1997, 2242
Mva1269I GAATGC 1 cut(s) 1858
MvaI CCWGG 4 cut(s) 680, 1134, 1818, 1997
MwoI GCNNNNNNNGC 6 cut(s) 186, 604, 724, 1390, 1997, 2615
NciI CCSGG 1 cut(s) 2242
NdeII GATC 9 cut(s) 744, 769, 802, 952, 1276, 1450, 2131, 2479, 2653
NlaIV GGNNCC 9 cut(s) 627, 869, 1114, 1115, 1533, 2102, 2514, 2535, 2553
NmeAIII GCCGAG 1 cut(s) 844
NmuCI GTSAC 6 cut(s) 49, 117, 749, 1820, 1924, 2236
NsbI TGCGCA 1 cut(s) 1992
NsiI ATGCAT 1 cut(s) 2192
NspI RCATGY 5 cut(s) 435, 718, 1598, 1870, 2190
NspV TTCGAA 1 cut(s) 319
OliI CACNNNNGTG 1 cut(s) 1595
PaeI GCATGC 2 cut(s) 435, 2190
PaqCI CACCTGC 1 cut(s) 1886
PceI AGGCCT 1 cut(s) 504
PciI ACATGT 1 cut(s) 1594
PctI GAATGC 1 cut(s) 1858
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 4 cut(s) 905, 1358, 1667, 2270
Pfl23II CGTACG 1 cut(s) 1632
PfoI TCCNGGA 1 cut(s) 1132
PinAI ACCGGT 1 cut(s) 2103
PkrI GCNGC 6 cut(s) 733, 1648, 2002, 2005, 2366, 2617
PleI GAGTC 1 cut(s) 1802
PmaCI CACGTG 2 cut(s) 122, 926
PmlI CACGTG 2 cut(s) 122, 926
PpsI GAGTC 1 cut(s) 1802
Ppu21I YACGTR 3 cut(s) 122, 300, 926
PpuMI RGGWCCY 1 cut(s) 1531
PscI ACATGT 1 cut(s) 1594
PshBI ATTAAT 2 cut(s) 2385, 2466
Psp5II RGGWCCY 1 cut(s) 1531
Psp6I CCWGG 4 cut(s) 678, 1132, 1816, 1995
PspCI CACGTG 2 cut(s) 122, 926
PspGI CCWGG 4 cut(s) 678, 1132, 1816, 1995
PspLI CGTACG 1 cut(s) 1632
PspN4I GGNNCC 9 cut(s) 627, 869, 1114, 1115, 1533, 2102, 2514, 2535, 2553
PspOMI GGGCCC 1 cut(s) 1112
PspPI GGNCC 6 cut(s) 489, 1112, 1113, 1531, 2166, 2533
PspPPI RGGWCCY 1 cut(s) 1531
PstI CTGCAG 1 cut(s) 2005
PstNI CAGNNNCTG 2 cut(s) 692, 1901
PsuI RGATCY 4 cut(s) 769, 952, 2131, 2479
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 7 cut(s) 423, 912, 1307, 1406, 1634, 1865, 2102
RsaNI GTAC 7 cut(s) 422, 911, 1306, 1405, 1633, 1864, 2101
RseI CAYNNNNRTG 3 cut(s) 719, 1595, 2406
SatI GCNGC 6 cut(s) 732, 1647, 2001, 2004, 2365, 2616
Sau3AI GATC 9 cut(s) 744, 769, 802, 952, 1276, 1450, 2131, 2479, 2653
Sau96I GGNCC 6 cut(s) 489, 1112, 1113, 1531, 2166, 2533
SchI GAGTC 1 cut(s) 1802
ScrFI CCNGG 5 cut(s) 680, 1134, 1818, 1997, 2242
SduI GDGCHC 4 cut(s) 550, 1116, 2124, 2152
SfaNI GCATC 6 cut(s) 714, 1504, 1637, 2065, 2199, 2536
SfcI CTRYAG 3 cut(s) 567, 1059, 2001
SfuI TTCGAA 1 cut(s) 319
SinI GGWCC 3 cut(s) 1531, 2166, 2533
SmiMI CAYNNNNRTG 3 cut(s) 719, 1595, 2406
SmlI CTYRAG 1 cut(s) 1825
SmoI CTYRAG 1 cut(s) 1825
SpeI ACTAGT 1 cut(s) 1330
SphI GCATGC 2 cut(s) 435, 2190
SseBI AGGCCT 1 cut(s) 504
SsiI CCGC 3 cut(s) 2111, 2549, 2564
SspI AATATT 1 cut(s) 1693
SspMI CTAG 4 cut(s) 1331, 2066, 2280, 2595
StuI AGGCCT 1 cut(s) 504
StyD4I CCNGG 5 cut(s) 678, 1132, 1816, 1995, 2240
StyI CCWWGG 3 cut(s) 629, 1641, 2169
TaaI ACNGT 8 cut(s) 167, 203, 426, 667, 694, 1565, 1779, 1846
TaiI ACGT 4 cut(s) 124, 302, 928, 2691
TaqI TCGA 6 cut(s) 319, 587, 1011, 2455, 2505, 2668
TaqII GACCGA 1 cut(s) 98
TatI WGTACW 2 cut(s) 910, 1863
TfiI GAWTC 4 cut(s) 905, 1358, 1667, 2270
TscAI CASTG 5 cut(s) 754, 1124, 1784, 1851, 2349
TseFI GTSAC 6 cut(s) 49, 117, 749, 1820, 1924, 2236
TseI GCWGC 6 cut(s) 731, 1646, 2000, 2003, 2364, 2615
Tsp45I GTSAC 6 cut(s) 49, 117, 749, 1820, 1924, 2236
TspGWI ACGGA 2 cut(s) 31, 768
TspRI CASTG 5 cut(s) 754, 1124, 1784, 1851, 2349
VpaK11BI GGWCC 3 cut(s) 1531, 2166, 2533
VspI ATTAAT 2 cut(s) 2385, 2466
XagI CCTNNNNNAGG 1 cut(s) 988
XapI RAATTY 4 cut(s) 253, 311, 1316, 1616
XbaI TCTAGA 1 cut(s) 2279
XceI RCATGY 5 cut(s) 435, 718, 1598, 1870, 2190
XmiI GTMKAC 1 cut(s) 2492
XmnI GAANNNNTTC 1 cut(s) 253
XspI CTAG 4 cut(s) 1331, 2066, 2280, 2595
Zsp2I ATGCAT 1 cut(s) 2192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.