Rorug06G0357400

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005367
Physical Location & Seq
Reverse (-)
50487362 .. 50488514
1153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0357400.1

Sequence Viewer

Length: 864 bp
ATGGCTCTCACTCGCTTCCTCCTCCCCACCTGTCTGGTTTTACTTTCATTGCTACTCATCAACGTCTCTGCAACTGATTATGGCTATGGCCCAAAACCACCACAGGTTGAGCAACCAAAACCTCCACAGGTCCAGAAACCAGAACCACCACAGGTCCAGAAACCAAAACCACCACAGGTTGAGAAACCAACACCTCCACAGGTCTCTGCTACTGATTATGGCTATGGCCCAAAACCACCACAGGTTGAGCAACCAAAACCTCCGCAGGTCCAGAAACCAAAACCACCACAGGTTGAGCAACCAAAACCTCCGCAGGTCCAGAAACCAAAACCACCACAGGTTGAGAAACCAACACCACCACAGGTCTCCACTAATGATTATGGCTATGGCCCAAAACCACCACAGGTTGAGAAACCAAAACTAGATCAGTTGTACGGAGAGCTACTACCTCACAATCTGTTCGGCATTCAAGGGCTTGTTTTATGTAACTCAGGACTTAAAGCCTTCCCAATTCAAGGAGCTGTGGCAAGGATTACATGCGTGGGCGAGGACGAAAATGGGTACGAGACTGCACCTTTCTCCATCTTAAGTGGTGCAACTGATGCAAAAGGCTACTTCTTTGCAACATTGTCACCTTCGAAGCTTGAAGGTAATTACAACAATAAGTGGAAGCTCACAGAGTGCAAGACATTCCTCGACAATTCTCCACTGGAGAGCTGCAAAGTCCCTACTGATGTCAACCATGGAATTAGTGGTGCTCCGATTGCTTCTTATCGCACTATCAATGCCAAGAACATGAAGCTGTTCTCTGTTGGACCCTTCTTCTACTCCTCAGAACCTAAACCAGCCCCTAGTGGCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

287

Amino Acids

31.32

Weight (kDa)

8.99

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 156 - 252 2.2e-17 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 256, 304
AciI CCGC 2 cut(s) 263, 311
AdeI CACNNNGTG 1 cut(s) 681
AfaI GTAC 2 cut(s) 434, 563
AfiI CCNNNNNNNGG 1 cut(s) 515
AflII CTTAAG 1 cut(s) 586
AgsI TTSAA 3 cut(s) 470, 515, 647
AluBI AGCT 6 cut(s) 442, 521, 643, 673, 717, 802
AluI AGCT 6 cut(s) 442, 521, 643, 673, 717, 802
Alw21I GWGCWC 1 cut(s) 760
Alw26I GTCTC 4 cut(s) 70, 208, 370, 560
AoxI GGCC 3 cut(s) 88, 226, 388
ApeKI GCWGC 1 cut(s) 717
Asp700I GAANNNNTTC 1 cut(s) 803
AspS9I GGNCC 8 cut(s) 89, 130, 154, 227, 268, 316, 389, 815
AsuHPI GGTGA 1 cut(s) 624
AsuII TTCGAA 1 cut(s) 638
AvaII GGWCC 5 cut(s) 130, 154, 268, 316, 815
Bbv12I GWGCWC 1 cut(s) 760
BbvI GCAGC 1 cut(s) 704
BccI CCATC 1 cut(s) 590
BcoDI GTCTC 4 cut(s) 70, 208, 370, 560
BfaI CTAG 2 cut(s) 422, 852
BfrI CTTAAG 1 cut(s) 586
BfuAI ACCTGC 2 cut(s) 256, 304
BisI GCNGC 1 cut(s) 718
BlsI GCNGC 1 cut(s) 719
Bme18I GGWCC 5 cut(s) 130, 154, 268, 316, 815
BmgT120I GGNCC 8 cut(s) 89, 130, 154, 227, 268, 316, 389, 815
BmiI GGNNCC 1 cut(s) 817
BmsI GCATC 1 cut(s) 592
BpmI CTGGAG 1 cut(s) 731
Bpu14I TTCGAA 1 cut(s) 638
BsaI GGTCTC 2 cut(s) 208, 370
BsaJI CCNNGG 1 cut(s) 742
Bsc4I CCNNNNNNNGG 1 cut(s) 515
Bse1I ACTGG 1 cut(s) 714
Bse3DI GCAATG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 742
BseLI CCNNNNNNNGG 1 cut(s) 515
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 2 cut(s) 504, 846
BseNI ACTGG 1 cut(s) 714
BseRI GAGGAG 2 cut(s) 11, 820
BseXI GCAGC 1 cut(s) 704
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 3 cut(s) 90, 228, 390
BsiHKAI GWGCWC 1 cut(s) 760
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 1 cut(s) 515
BsmAI GTCTC 4 cut(s) 70, 208, 370, 560
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 710
BsmI GAATGC 1 cut(s) 465
BsnI GGCC 3 cut(s) 90, 228, 390
Bso31I GGTCTC 2 cut(s) 208, 370
Bsp119I TTCGAA 1 cut(s) 638
Bsp1286I GDGCHC 1 cut(s) 760
Bsp143I GATC 1 cut(s) 424
Bsp19I CCATGG 1 cut(s) 742
BspACI CCGC 2 cut(s) 263, 311
BspANI GGCC 3 cut(s) 90, 228, 390
BspCNI CTCAG 2 cut(s) 503, 845
BspLI GGNNCC 1 cut(s) 817
BspMI ACCTGC 2 cut(s) 256, 304
BspT104I TTCGAA 1 cut(s) 638
BspTI CTTAAG 1 cut(s) 586
BspTNI GGTCTC 2 cut(s) 208, 370
BsrDI GCAATG 1 cut(s) 47
BsrI ACTGG 1 cut(s) 714
BssECI CCNNGG 1 cut(s) 742
BssMI GATC 1 cut(s) 424
BssT1I CCWWGG 1 cut(s) 742
BstAFI CTTAAG 1 cut(s) 586
BstAPI GCANNNNNTGC 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 638
BstDEI CTNAG 2 cut(s) 490, 832
BstDSI CCRYGG 1 cut(s) 742
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 4 cut(s) 70, 208, 370, 560
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 2 cut(s) 602, 764
BstNSI RCATGY 1 cut(s) 540
BstV1I GCAGC 1 cut(s) 704
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 3 cut(s) 90, 228, 390
BtgI CCRYGG 1 cut(s) 742
BtsIMutI CAGTG 1 cut(s) 707
BveI ACCTGC 2 cut(s) 256, 304
Cfr13I GGNCC 8 cut(s) 89, 130, 154, 227, 268, 316, 389, 815
Csp6I GTAC 2 cut(s) 433, 562
CviAII CATG 3 cut(s) 537, 743, 796
CviQI GTAC 2 cut(s) 433, 562
DdeI CTNAG 2 cut(s) 490, 832
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
DraIII CACNNNGTG 1 cut(s) 681
Eco130I CCWWGG 1 cut(s) 742
Eco31I GGTCTC 2 cut(s) 208, 370
Eco47I GGWCC 5 cut(s) 130, 154, 268, 316, 815
EcoT14I CCWWGG 1 cut(s) 742
ErhI CCWWGG 1 cut(s) 742
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 3 cut(s) 540, 746, 799
FaqI GGGAC 1 cut(s) 710
FatI CATG 3 cut(s) 536, 742, 795
Fnu4HI GCNGC 1 cut(s) 718
Fsp4HI GCNGC 1 cut(s) 718
FspBI CTAG 2 cut(s) 422, 852
GluI GCNGC 1 cut(s) 718
GsuI CTGGAG 1 cut(s) 731
HaeIII GGCC 3 cut(s) 90, 228, 390
Hin1II CATG 3 cut(s) 540, 746, 799
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 641
HphI GGTGA 1 cut(s) 624
Hpy166II GTNNAC 1 cut(s) 739
Hpy188I TCNGA 2 cut(s) 762, 835
Hpy188III TCNNGA 5 cut(s) 133, 157, 271, 319, 492
Hpy8I GTNNAC 1 cut(s) 739
HpyAV CCTTC 4 cut(s) 514, 641, 645, 829
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 7 cut(s) 71, 572, 596, 605, 623, 684, 720
HpyF10VI GCNNNNNNNGC 2 cut(s) 602, 764
HpyF3I CTNAG 2 cut(s) 490, 832
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 3 cut(s) 540, 746, 799
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 2 cut(s) 518, 763
Lsp1109I GCAGC 1 cut(s) 704
LweI GCATC 1 cut(s) 592
MaeI CTAG 2 cut(s) 422, 852
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 2 cut(s) 485, 630
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 1 cut(s) 814
MhlI GDGCHC 1 cut(s) 760
MluCI AATT 4 cut(s) 510, 652, 700, 747
MmeI TCCRAC 1 cut(s) 793
MroXI GAANNNNTTC 1 cut(s) 803
MseI TTAA 2 cut(s) 498, 587
MspCI CTTAAG 1 cut(s) 586
Mva1269I GAATGC 1 cut(s) 465
MwoI GCNNNNNNNGC 2 cut(s) 602, 764
NcoI CCATGG 1 cut(s) 742
NdeII GATC 1 cut(s) 424
NlaIII CATG 3 cut(s) 540, 746, 799
NlaIV GGNNCC 1 cut(s) 817
NmuCI GTSAC 1 cut(s) 630
NspI RCATGY 1 cut(s) 540
NspV TTCGAA 1 cut(s) 638
PctI GAATGC 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 803
PkrI GCNGC 1 cut(s) 719
PspN4I GGNNCC 1 cut(s) 817
PspPI GGNCC 8 cut(s) 89, 130, 154, 227, 268, 316, 389, 815
RsaI GTAC 2 cut(s) 434, 563
RsaNI GTAC 2 cut(s) 433, 562
SaqAI TTAA 2 cut(s) 498, 587
SatI GCNGC 1 cut(s) 718
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 8 cut(s) 89, 130, 154, 227, 268, 316, 389, 815
SduI GDGCHC 1 cut(s) 760
SfaNI GCATC 1 cut(s) 592
SfuI TTCGAA 1 cut(s) 638
SinI GGWCC 5 cut(s) 130, 154, 268, 316, 815
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
Sse9I AATT 4 cut(s) 510, 652, 700, 747
SsiI CCGC 2 cut(s) 263, 311
SspMI CTAG 2 cut(s) 422, 852
StyI CCWWGG 1 cut(s) 742
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 638, 696
TasI AATT 4 cut(s) 510, 652, 700, 747
Tru1I TTAA 2 cut(s) 498, 587
Tru9I TTAA 2 cut(s) 498, 587
TscAI CASTG 1 cut(s) 714
TseFI GTSAC 1 cut(s) 630
TseI GCWGC 1 cut(s) 717
Tsp45I GTSAC 1 cut(s) 630
TspDTI ATGAA 2 cut(s) 36, 812
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 1 cut(s) 714
Vha464I CTTAAG 1 cut(s) 586
VpaK11BI GGWCC 5 cut(s) 130, 154, 268, 316, 815
XceI RCATGY 1 cut(s) 540
XcmI CCANNNNNNNNNTGG 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 803
XspI CTAG 2 cut(s) 422, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.