MD15G1086800.v1.1

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005367
Physical Location & Seq
Reverse (-)
6014420 .. 6022007
7588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1086800.v1.1.491

Sequence Viewer

Length: 3066 bp
ATGGGGTCGGATGTGCAGTCCGTTGGTTTTCTAGACACTTTAAAAATGGAGAGGGTTAGGACAATTCTAACCCATACATATCCTTATCCGCATGAACATTCCCGTCATGCCGTAATTGCTGTTGTTGTGGGTTGCCTCTTTTTTATCTCATCCGACAACATCAACACCCTTATAGAAAAGTTAGACAACAATATTAAATGGTGGTCTATGTATGCATGCTTGCTGGGTTTCTTCTATTTCTTTTCGTCCCCATTTATAGGGAAGACTATCAAACCGAGCTATTCAAATTTCAGTAGGTGGTATATACTCTGGATTCTAGTTGCGGCTGTCTATCATCTTCCTAGTTTTCAATCAATGGGAGTTGATATGAGGATGAATCTGTCTTTGGGCTTTAATGTATTTGTCACTTCTGTTTTGTTTCTTCTTTTCTTCCACATTATATTCATTGGCCTTTGGTACATTGGTCTCGTGTCGCGTGTTGCTGGAAGGAGGCCAGCAATTTTGACCATTCTCCAAAATTGTGCTGTTCTTAGCGTAGCCTGCTGTGTGTTTTATAGTCATTGTGGCAACCGTGCTATTCTGAGAGATAGACAACTGGTGCGACGAAACTCTTGGTTTAATTTCTGGAAGAAAGATGAGAGGAACACATGGCTTTCGAAGTTCCTCCGAATGAATGAGTTGAAAGATCAGGTCTGCTCATCTTGGTTTGCTCCAGTTGGATCTGCAAGTGATTATCCACTTTTGTCCAAATGGGTCATTTATGGTGAGTTAGCATGCAATGGTGCATGTGCTGGTTCATCGGATGAAATCTCCCCATTATACTCGTTATGGGCCACATTCATAGGCCTCTACATTGCTAATTATGTAGTGGAGAGATCAACAGGGTGGGCTCTTACTCACCCCCTGTCTGTTGAAGAACGTCAGAAGTCGAAGAAGAAGCAAATGAAGCCAGATTTTTTGGATATGGTTCCTTGGTATTCAGGAACATCAGCTGATTTATTCAAGACTGTCTTTGACCTCTTGGTATCAGTAACCGTCTTTGTTGGCCGCTTTGACATGCGTATGATGCAGGCAGCTATGGACAAGGTCCATGATGGAGCCAAACAAAAGGATCTTTTATTTGATAACTTTGCCGAGAGGGATGATTTATGGTTTGACTTCATGGCTGATACTGGCGATGGTGGAAACTCATCTTATTCTGTGGCTCGGCTCCTTGCTCAACCTTCCATTAATATTAACAGAGATGATTCCATGCTGAAGTTACCACGTGGAGACTTGCTTCTTATTGGAGGGGATCTTGCGTATCCTAATCCATCATCTTTCACGTATGAAAGGCGCCTCTTTTGTCCTTTTGAGTATGCTCTCCAGCCTCCTCCATGGTCTAAACAAGAGCATATAGCTGTAGATAAGCCTGAACTACCTCGTGGTGTGTCTGAACTGAAGAAATACGATGGGCCTCAGTGTTTTGTCATTCCTGGAAACCATGATTGGTTTGATGGACTTAATACATTCATGAGGTATATATGCCATAAAAACTGGTTGGGTGGGTGGTTTATGCCTCAAAAGAAGAGTTATTTTGCTCTTCAACTCCCTAAAAGATGGTGGGTATTTGGTTTTGATCTCGCACTCCATGGTGATATTGATGTGTACCAGTTCAAATTCTTTACAGAACTTATTAAGAACAAGGTTAGAAATGGCGACTCTGTAATCATTATGACGCATGAACCAAATTGGCTTCTTGATTGGTACTGGAATGATGTTTCTGGAAAGAATGTTGCGCACCTGATATGCGATTATTTAAAAGGGAGATGTAAGCTTCGAGTGGCTGGAGATTTGCATCATTATATGCGTCATTCATTTATTAAAACAGATGGCCCAGTTCCCGTGCAACATTTAATTGTAAATGGTTGCGGTGGGGCTTTTCTACATCCCACTCACACTTTTAGTAATTTTAAGAAATTTTGTGGAGTTTCTTATGAGACCAAGGCTGCTTATCCTTCATTTGAAGATTCAAGCAGGATTGCTCTGGGAAATATTTTAAAGTTTCGAAAGAAGAACTGGCAATTTGATTTTATTGGTGGAATTATATACTTCATATTGGTTTTTTCTATGTTCCCACAGTGTAAGCTTGGTCATATCCTGCGAGAAGATTCATTTTCTGGTCACGTGGGGTCTTTCTTTCAAACAGTGTGGAATGCTTTTATGTACATGCTGGCACGCTCTTATGTATCTTTAGCAGGTGCCCTGCTGCTGCTAATTGTGGCAGTTACATTTGTCCCCTCTAAGGTGTCTCGGAAGAAACGATTGATGATTGGAGTTCTTCATGTGTCTGCGCACCTGGCTGCAGCTCTAATTCTTATGTTACTGTTGGAACTGGGTGTTGAGATGTGCATCCAGCATAAACTACTGGGAACTTCAGGCTATCATACTTTATATCAGTGGTACCGATCGGTGGAGAGTGAGCACTTTCCAGATCCAACTGGCCTTCGAGCTCGTATAGAACAATGGACTTTTGGCCTTTATCCAGCATGCATCAAGTATTTCATGTCTGCATTTGATGTTCCAGAGGTTATGGCTGTCACCCGAAACAATATATGCAAGACTGGGATGGAATCACTATCTAGAGCTGGTGGTGTCATATATTATGCTTCCGTGTTCCTCTATTTCTGGGTCTTCTCGACTCCAGTAGTTTCTTTGGTGTTTGGAAGTTATTTATACATCTGCATTAATTGGCTTCACATACACTTCGATGAAGCCTTTTCCTCTCTCCGAATTGCAAATTACAAGTCTTTCACCCGATTTCACATCAACAGTGACGGCGATCTTGAAGTTTACACTCTTGCAGTCGATAAGGTCCCCAAAAAATGGAAGCTAGACCCTGAATGGGATCGGGAGCCAAAACAGCCGGAACAAATGAGCCACCTTAGAAAATTTCCGAGCAAATGGAGTGCGTCAGCTGGTCAACAAGATCCGTTAAATACAGTTAAAATCGTTGATCAGTTTGTTATTCGACAAACAGATAAAACTGATGTTGGAGCAAGTGATGCATCAGTAATATTATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

1022

Amino Acids

117.33

Weight (kDa)

8.84

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 387 - 588 2.8e-06 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2228
Acc16I TGCGCA 2 cut(s) 1779, 2334
Acc36I ACCTGC 1 cut(s) 2228
Acc65I GGTACC 1 cut(s) 2442
AccB1I GGYRCC 3 cut(s) 1335, 2240, 2442
AccB7I CCANNNNNTGG 1 cut(s) 2865
AccII CGCG 1 cut(s) 475
AciI CCGC 4 cut(s) 89, 323, 1048, 1911
AclWI GGATC 6 cut(s) 727, 1119, 1302, 2468, 2895, 2963
AcoI YGGCCR 1 cut(s) 1045
AcsI RAATTY 4 cut(s) 286, 1658, 1958, 2930
AcuI CTGAAG 3 cut(s) 1277, 1460, 2400
AcvI CACGTG 2 cut(s) 1268, 2167
AcyI GRCGYC 1 cut(s) 1336
AfaI GTAC 5 cut(s) 458, 1649, 1748, 2207, 2444
AfiI CCNNNNNNNGG 5 cut(s) 257, 2284, 2452, 2865, 2884
AjnI CCWGG 2 cut(s) 1474, 2337
AjuI GAANNNNNNNTTGG 4 cut(s) 368, 400, 1471, 1503
Alw21I GWGCWC 2 cut(s) 2466, 2494
Alw26I GTCTC 4 cut(s) 470, 1266, 1973, 2295
AlwI GGATC 6 cut(s) 727, 1119, 1302, 2468, 2895, 2963
AoxI GGCC 9 cut(s) 448, 491, 831, 844, 1045, 1454, 1873, 2482, 2515
ApeKI GCWGC 6 cut(s) 1073, 1988, 2248, 2251, 2342, 2345
ApoI RAATTY 4 cut(s) 286, 1658, 1958, 2930
ArsI GACNNNNNNTTYG 2 cut(s) 2771, 2803
AseI ATTAAT 2 cut(s) 1230, 2727
Asp718I GGTACC 1 cut(s) 2442
AspLEI GCGC 3 cut(s) 1338, 1780, 2335
AspS9I GGNCC 5 cut(s) 831, 1087, 1454, 1874, 2854
AsuHPI GGTGA 5 cut(s) 776, 890, 1646, 2572, 2785
AsuII TTCGAA 2 cut(s) 656, 2047
AvaII GGWCC 2 cut(s) 1087, 2854
BaeGI GKGCMC 1 cut(s) 2245
BaeI ACNNNNGTAYC 2 cut(s) 448, 481
BanI GGYRCC 3 cut(s) 1335, 2240, 2442
BanII GRGCYC 2 cut(s) 892, 2494
BauI CACGAG 2 cut(s) 467, 1422
BbrPI CACGTG 2 cut(s) 1268, 2167
BbsI GAAGAC 2 cut(s) 269, 2665
Bbv12I GWGCWC 2 cut(s) 2466, 2494
BbvI GCAGC 6 cut(s) 1085, 1975, 2235, 2238, 2329, 2357
BccI CCATC 8 cut(s) 1088, 1172, 1321, 1445, 1490, 1593, 1865, 2602
BceAI ACGGC 2 cut(s) 95, 2833
BciT130I CCWGG 2 cut(s) 1476, 2339
BciVI GTATCC 1 cut(s) 1314
BclI TGATCA 1 cut(s) 2995
BcoDI GTCTC 4 cut(s) 470, 1266, 1973, 2295
BfaI CTAG 5 cut(s) 32, 317, 342, 2622, 2873
BfmI CTRYAG 2 cut(s) 1401, 2343
BfoI RGCGCY 1 cut(s) 1339
BfuAI ACCTGC 1 cut(s) 2228
BfuI GTATCC 1 cut(s) 1314
BisI GCNGC 8 cut(s) 324, 1048, 1074, 1989, 2249, 2252, 2343, 2346
BlsI GCNGC 8 cut(s) 325, 1049, 1075, 1990, 2250, 2253, 2344, 2347
Bme1390I CCNGG 2 cut(s) 1476, 2339
Bme18I GGWCC 2 cut(s) 1087, 2854
BmgT120I GGNCC 5 cut(s) 831, 1087, 1454, 1874, 2854
BmiI GGNNCC 8 cut(s) 969, 1099, 1211, 1337, 2242, 2444, 2856, 2895
BmrFI CCNGG 2 cut(s) 1476, 2339
BmrI ACTGGG 4 cut(s) 1871, 2384, 2417, 2613
BmsI GCATC 6 cut(s) 1056, 1846, 2400, 2541, 3034, 3056
BmuI ACTGGG 4 cut(s) 1871, 2384, 2417, 2613
BpiI GAAGAC 2 cut(s) 269, 2665
BpmI CTGGAG 4 cut(s) 696, 1349, 1848, 2667
Bpu14I TTCGAA 2 cut(s) 656, 2047
BsaAI YACGTR 3 cut(s) 1268, 1326, 2167
BsaBI GATNNNNATC 2 cut(s) 1836, 2390
BsaHI GRCGYC 1 cut(s) 1336
BsaI GGTCTC 2 cut(s) 470, 1973
BsaJI CCNNGG 4 cut(s) 971, 1376, 1630, 1983
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 2284, 2452, 2865, 2884
Bse3DI GCAATG 2 cut(s) 784, 852
Bse8I GATNNNNATC 2 cut(s) 1836, 2390
BseBI CCWGG 2 cut(s) 1476, 2339
BseDI CCNNGG 4 cut(s) 971, 1376, 1630, 1983
BseGI GGATG 8 cut(s) 16, 149, 378, 808, 1147, 1927, 2391, 2613
BseJI GATNNNNATC 2 cut(s) 1836, 2390
BseLI CCNNNNNNNGG 5 cut(s) 257, 2284, 2452, 2865, 2884
BseMI GCAATG 2 cut(s) 784, 852
BseMII CTCAG 2 cut(s) 572, 1472
BseRI GAGGAG 1 cut(s) 1362
BseSI GKGCMC 1 cut(s) 2245
BseXI GCAGC 6 cut(s) 1085, 1975, 2235, 2238, 2329, 2357
BseYI CCCAGC 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 475
Bsh1285I CGRYCG 1 cut(s) 2450
BshFI GGCC 9 cut(s) 450, 493, 833, 846, 1047, 1456, 1875, 2484, 2517
BshNI GGYRCC 3 cut(s) 1335, 2240, 2442
BsiEI CGRYCG 1 cut(s) 2450
BsiHKAI GWGCWC 2 cut(s) 2466, 2494
BsiSI CCGG 1 cut(s) 2906
BslFI GGGAC 3 cut(s) 232, 2261, 2840
BslI CCNNNNNNNGG 5 cut(s) 257, 2284, 2452, 2865, 2884
BsmAI GTCTC 4 cut(s) 470, 1266, 1973, 2295
BsmFI GGGAC 3 cut(s) 232, 2261, 2840
BsmI GAATGC 1 cut(s) 2200
BsnI GGCC 9 cut(s) 450, 493, 833, 846, 1047, 1456, 1875, 2484, 2517
Bso31I GGTCTC 2 cut(s) 470, 1973
Bsp119I TTCGAA 2 cut(s) 656, 2047
Bsp1286I GDGCHC 4 cut(s) 892, 2245, 2466, 2494
Bsp1407I TGTACA 1 cut(s) 2205
Bsp19I CCATGG 2 cut(s) 1376, 1630
BspACI CCGC 4 cut(s) 89, 323, 1048, 1911
BspANI GGCC 9 cut(s) 450, 493, 833, 846, 1047, 1456, 1875, 2484, 2517
BspCNI CTCAG 2 cut(s) 573, 1471
BspFNI CGCG 1 cut(s) 475
BspHI TCATGA 1 cut(s) 1512
BspLI GGNNCC 8 cut(s) 969, 1099, 1211, 1337, 2242, 2444, 2856, 2895
BspMAI CTGCAG 1 cut(s) 2347
BspMI ACCTGC 1 cut(s) 2228
BspPI GGATC 6 cut(s) 727, 1119, 1302, 2468, 2895, 2963
BspQI GCTCTTC 1 cut(s) 1587
BspT104I TTCGAA 2 cut(s) 656, 2047
BspT107I GGYRCC 3 cut(s) 1335, 2240, 2442
BspTNI GGTCTC 2 cut(s) 470, 1973
BsrDI GCAATG 2 cut(s) 784, 852
BsrGI TGTACA 1 cut(s) 2205
BssECI CCNNGG 4 cut(s) 971, 1376, 1630, 1983
BssNI GRCGYC 1 cut(s) 1336
BssSI CACGAG 2 cut(s) 467, 1422
BssT1I CCWWGG 4 cut(s) 971, 1376, 1630, 1983
Bst2BI CACGAG 2 cut(s) 467, 1422
Bst2UI CCWGG 2 cut(s) 1476, 2339
Bst4CI ACNGT 8 cut(s) 572, 1009, 1036, 2121, 2188, 2367, 2813, 2983
Bst6I CTCTTC 2 cut(s) 1562, 1587
BstACI GRCGYC 1 cut(s) 1336
BstAPI GCANNNNNTGC 1 cut(s) 3044
BstAUI TGTACA 1 cut(s) 2205
BstBAI YACGTR 3 cut(s) 1268, 1326, 2167
BstBI TTCGAA 2 cut(s) 656, 2047
BstC8I GCNNGC 9 cut(s) 217, 221, 495, 541, 775, 1071, 2214, 2218, 2530
BstDEI CTNAG 5 cut(s) 530, 581, 1458, 2283, 2924
BstDSI CCRYGG 2 cut(s) 1376, 1630
BstF5I GGATG 8 cut(s) 16, 149, 378, 808, 1147, 1927, 2391, 2613
BstFNI CGCG 1 cut(s) 475
BstH2I RGCGCY 1 cut(s) 1339
BstHHI GCGC 3 cut(s) 1338, 1780, 2335
BstMAI GTCTC 4 cut(s) 470, 1266, 1973, 2295
BstMCI CGRYCG 1 cut(s) 2450
BstMWI GCNNNNNNNGC 7 cut(s) 116, 540, 946, 1066, 2339, 2902, 3044
BstNI CCWGG 2 cut(s) 1476, 2339
BstNSI RCATGY 6 cut(s) 219, 777, 789, 1060, 2212, 2532
BstSCI CCNGG 2 cut(s) 1474, 2337
BstSFI CTRYAG 2 cut(s) 1401, 2343
BstSLI GKGCMC 1 cut(s) 2245
BstUI CGCG 1 cut(s) 475
BstV1I GCAGC 6 cut(s) 1085, 1975, 2235, 2238, 2329, 2357
BstV2I GAAGAC 2 cut(s) 269, 2665
BstX2I RGATCY 5 cut(s) 719, 1111, 1294, 2473, 2968
BstYI RGATCY 5 cut(s) 719, 1111, 1294, 2473, 2968
BsuI GTATCC 1 cut(s) 1314
BsuRI GGCC 9 cut(s) 450, 493, 833, 846, 1047, 1456, 1875, 2484, 2517
BtgI CCRYGG 2 cut(s) 1376, 1630
BtgZI GCGATG 1 cut(s) 1191
BtsCI GGATG 8 cut(s) 16, 149, 378, 808, 1147, 1927, 2391, 2613
BtsIMutI CAGTG 5 cut(s) 1466, 2126, 2193, 2444, 2818
BveI ACCTGC 1 cut(s) 2228
Cac8I GCNNGC 9 cut(s) 217, 221, 495, 541, 775, 1071, 2214, 2218, 2530
CciI TCATGA 1 cut(s) 1512
CfoI GCGC 3 cut(s) 1338, 1780, 2335
Cfr13I GGNCC 5 cut(s) 831, 1087, 1454, 1874, 2854
CseI GACGC 3 cut(s) 1726, 1838, 2940
Csp6I GTAC 5 cut(s) 457, 1648, 1747, 2206, 2443
CspCI CAANNNNNGTGG 2 cut(s) 2171, 2206
CviQI GTAC 5 cut(s) 457, 1648, 1747, 2206, 2443
DdeI CTNAG 5 cut(s) 530, 581, 1458, 2283, 2924
DinI GGCGCC 1 cut(s) 1337
DraI TTTAAA 3 cut(s) 42, 1800, 2040
EaeI YGGCCR 1 cut(s) 1045
Eam1104I CTCTTC 2 cut(s) 1562, 1587
EarI CTCTTC 2 cut(s) 1562, 1587
Ecl136II GAGCTC 1 cut(s) 2492
Eco130I CCWWGG 4 cut(s) 971, 1376, 1630, 1983
Eco147I AGGCCT 1 cut(s) 846
Eco24I GRGCYC 2 cut(s) 892, 2494
Eco31I GGTCTC 2 cut(s) 470, 1973
Eco47I GGWCC 2 cut(s) 1087, 2854
Eco53kI GAGCTC 1 cut(s) 2492
Eco57I CTGAAG 3 cut(s) 1277, 1460, 2400
Eco72I CACGTG 2 cut(s) 1268, 2167
EcoICRI GAGCTC 1 cut(s) 2492
EcoO109I RGGNCCY 1 cut(s) 2854
EcoRII CCWGG 2 cut(s) 1474, 2337
EcoT14I CCWWGG 4 cut(s) 971, 1376, 1630, 1983
EcoT22I ATGCAT 3 cut(s) 217, 2534, 3049
EcoT38I GRGCYC 2 cut(s) 892, 2494
EgeI GGCGCC 1 cut(s) 1337
EheI GGCGCC 1 cut(s) 1337
ErhI CCWWGG 4 cut(s) 971, 1376, 1630, 1983
FalI AAGNNNNNCTT 2 cut(s) 995, 1027
FaqI GGGAC 3 cut(s) 232, 2261, 2840
FbaI TGATCA 1 cut(s) 2995
Fnu4HI GCNGC 8 cut(s) 324, 1048, 1074, 1989, 2249, 2252, 2343, 2346
FokI GGATG 8 cut(s) 23, 136, 385, 815, 1154, 1914, 2378, 2620
FriOI GRGCYC 2 cut(s) 892, 2494
Fsp4HI GCNGC 8 cut(s) 324, 1048, 1074, 1989, 2249, 2252, 2343, 2346
FspBI CTAG 5 cut(s) 32, 317, 342, 2622, 2873
FspI TGCGCA 2 cut(s) 1779, 2334
GlaI GCGC 3 cut(s) 1337, 1779, 2334
GluI GCNGC 8 cut(s) 324, 1048, 1074, 1989, 2249, 2252, 2343, 2346
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 4 cut(s) 696, 1349, 1848, 2667
HaeII RGCGCY 1 cut(s) 1339
HaeIII GGCC 9 cut(s) 450, 493, 833, 846, 1047, 1456, 1875, 2484, 2517
HapII CCGG 1 cut(s) 2906
HgaI GACGC 3 cut(s) 1726, 1838, 2940
HhaI GCGC 3 cut(s) 1338, 1780, 2335
Hin1I GRCGYC 1 cut(s) 1336
Hin6I GCGC 3 cut(s) 1336, 1778, 2333
HinP1I GCGC 3 cut(s) 1336, 1778, 2333
HincII GTYRAC 1 cut(s) 2963
HindII GTYRAC 1 cut(s) 2963
HindIII AAGCTT 2 cut(s) 1814, 2126
HinfI GANTC 8 cut(s) 313, 376, 1247, 1700, 2009, 2150, 2612, 2680
HpaII CCGG 1 cut(s) 2906
HphI GGTGA 5 cut(s) 776, 890, 1646, 2572, 2785
Hpy166II GTNNAC 3 cut(s) 1648, 2833, 2963
Hpy8I GTNNAC 3 cut(s) 1648, 2833, 2963
Hpy99I CGWCG 1 cut(s) 606
HpyAV CCTTC 4 cut(s) 480, 1233, 2007, 2495
HpyCH4III ACNGT 8 cut(s) 572, 1009, 1036, 2121, 2188, 2367, 2813, 2983
HpyCH4IV ACGT 4 cut(s) 919, 1267, 1325, 2166
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 540, 946, 1066, 2339, 2902, 3044
HpyF3I CTNAG 5 cut(s) 530, 581, 1458, 2283, 2924
HpySE526I ACGT 4 cut(s) 919, 1267, 1325, 2166
Hsp92I GRCGYC 1 cut(s) 1336
HspAI GCGC 3 cut(s) 1336, 1778, 2333
KasI GGCGCC 1 cut(s) 1335
KpnI GGTACC 1 cut(s) 2446
Ksp22I TGATCA 1 cut(s) 2995
LguI GCTCTTC 1 cut(s) 1587
LmnI GCTCC 5 cut(s) 715, 1097, 1215, 2893, 3035
Lsp1109I GCAGC 6 cut(s) 1085, 1975, 2235, 2238, 2329, 2357
LweI GCATC 6 cut(s) 1056, 1846, 2400, 2541, 3034, 3056
MaeI CTAG 5 cut(s) 32, 317, 342, 2622, 2873
MaeII ACGT 4 cut(s) 919, 1267, 1325, 2166
MaeIII GTNAC 8 cut(s) 403, 1030, 1260, 2162, 2266, 2361, 2578, 2813
MflI RGATCY 5 cut(s) 719, 1111, 1294, 2473, 2968
MhlI GDGCHC 4 cut(s) 892, 2245, 2466, 2494
Mly113I GGCGCC 1 cut(s) 1336
MlyI GAGTC 2 cut(s) 1694, 2674
MmeI TCCRAC 5 cut(s) 177, 697, 2349, 2501, 3013
Mph1103I ATGCAT 3 cut(s) 217, 2534, 3049
MslI CAYNNNNRTG 2 cut(s) 1061, 2748
MspA1I CMGCKG 2 cut(s) 992, 2957
MspI CCGG 1 cut(s) 2906
MspR9I CCNGG 2 cut(s) 1476, 2339
Mva1269I GAATGC 1 cut(s) 2200
MvaI CCWGG 2 cut(s) 1476, 2339
MvnI CGCG 1 cut(s) 475
MwoI GCNNNNNNNGC 7 cut(s) 116, 540, 946, 1066, 2339, 2902, 3044
NarI GGCGCC 1 cut(s) 1336
NcoI CCATGG 2 cut(s) 1376, 1630
NlaIV GGNNCC 8 cut(s) 969, 1099, 1211, 1337, 2242, 2444, 2856, 2895
NmeAIII GCCGAG 2 cut(s) 1159, 1186
NmuCI GTSAC 4 cut(s) 403, 2162, 2578, 2813
NsbI TGCGCA 2 cut(s) 1779, 2334
NsiI ATGCAT 3 cut(s) 217, 2534, 3049
NspI RCATGY 6 cut(s) 219, 777, 789, 1060, 2212, 2532
NspV TTCGAA 2 cut(s) 656, 2047
PaeI GCATGC 3 cut(s) 219, 777, 2532
PagI TCATGA 1 cut(s) 1512
PaqCI CACCTGC 1 cut(s) 2228
PceI AGGCCT 1 cut(s) 846
PciSI GCTCTTC 1 cut(s) 1587
PcsI WCGNNNNNNNCGW 1 cut(s) 2299
PctI GAATGC 1 cut(s) 2200
PfeI GAWTC 6 cut(s) 313, 376, 1247, 2009, 2150, 2612
PflFI GACNNNGTC 1 cut(s) 1085
PflMI CCANNNNNTGG 1 cut(s) 2865
PfoI TCCNGGA 1 cut(s) 1474
PkrI GCNGC 8 cut(s) 325, 1049, 1075, 1990, 2250, 2253, 2344, 2347
Ple19I CGATCG 1 cut(s) 2450
PleI GAGTC 2 cut(s) 1694, 2674
PluTI GGCGCC 1 cut(s) 1339
PmaCI CACGTG 2 cut(s) 1268, 2167
PmlI CACGTG 2 cut(s) 1268, 2167
PpsI GAGTC 2 cut(s) 1694, 2674
Ppu21I YACGTR 3 cut(s) 1268, 1326, 2167
PpuMI RGGWCCY 1 cut(s) 2854
PshBI ATTAAT 2 cut(s) 1230, 2727
Psp124BI GAGCTC 1 cut(s) 2494
Psp5II RGGWCCY 1 cut(s) 2854
Psp6I CCWGG 2 cut(s) 1474, 2337
PspCI CACGTG 2 cut(s) 1268, 2167
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 2 cut(s) 1474, 2337
PspN4I GGNNCC 8 cut(s) 969, 1099, 1211, 1337, 2242, 2444, 2856, 2895
PspPI GGNCC 5 cut(s) 831, 1087, 1454, 1874, 2854
PspPPI RGGWCCY 1 cut(s) 2854
PstI CTGCAG 1 cut(s) 2347
PsuI RGATCY 5 cut(s) 719, 1111, 1294, 2473, 2968
PsyI GACNNNGTC 1 cut(s) 1085
PvuI CGATCG 1 cut(s) 2450
PvuII CAGCTG 2 cut(s) 992, 2957
RsaI GTAC 5 cut(s) 458, 1649, 1748, 2207, 2444
RsaNI GTAC 5 cut(s) 457, 1648, 1747, 2206, 2443
RseI CAYNNNNRTG 2 cut(s) 1061, 2748
SacI GAGCTC 1 cut(s) 2494
SapI GCTCTTC 1 cut(s) 1587
SatI GCNGC 8 cut(s) 324, 1048, 1074, 1989, 2249, 2252, 2343, 2346
Sau96I GGNCC 5 cut(s) 831, 1087, 1454, 1874, 2854
SchI GAGTC 2 cut(s) 1694, 2674
ScrFI CCNGG 2 cut(s) 1476, 2339
SduI GDGCHC 4 cut(s) 892, 2245, 2466, 2494
SfaNI GCATC 6 cut(s) 1056, 1846, 2400, 2541, 3034, 3056
SfcI CTRYAG 2 cut(s) 1401, 2343
SfoI GGCGCC 1 cut(s) 1337
SfuI TTCGAA 2 cut(s) 656, 2047
SinI GGWCC 2 cut(s) 1087, 2854
SmiMI CAYNNNNRTG 2 cut(s) 1061, 2748
SphI GCATGC 3 cut(s) 219, 777, 2532
SseBI AGGCCT 1 cut(s) 846
SsiI CCGC 4 cut(s) 89, 323, 1048, 1911
SspDI GGCGCC 1 cut(s) 1335
SspI AATATT 4 cut(s) 193, 1234, 2035, 3057
SspMI CTAG 5 cut(s) 32, 317, 342, 2622, 2873
SstI GAGCTC 1 cut(s) 2494
StuI AGGCCT 1 cut(s) 846
StyD4I CCNGG 2 cut(s) 1474, 2337
StyI CCWWGG 4 cut(s) 971, 1376, 1630, 1983
TaaI ACNGT 8 cut(s) 572, 1009, 1036, 2121, 2188, 2367, 2813, 2983
TaiI ACGT 4 cut(s) 922, 1270, 1328, 2169
TaqI TCGA 9 cut(s) 656, 929, 1819, 2047, 2488, 2678, 2748, 2847, 3010
TatI WGTACW 1 cut(s) 2205
TauI GCSGC 2 cut(s) 326, 1050
TfiI GAWTC 6 cut(s) 313, 376, 1247, 2009, 2150, 2612
TscAI CASTG 5 cut(s) 1466, 2126, 2193, 2444, 2818
TseFI GTSAC 4 cut(s) 403, 2162, 2578, 2813
TseI GCWGC 6 cut(s) 1073, 1988, 2248, 2251, 2342, 2345
Tsp45I GTSAC 4 cut(s) 403, 2162, 2578, 2813
TspGWI ACGGA 3 cut(s) 10, 2641, 2961
TspRI CASTG 5 cut(s) 1466, 2126, 2193, 2444, 2818
Tth111I GACNNNGTC 1 cut(s) 1085
Van91I CCANNNNNTGG 1 cut(s) 2865
VpaK11BI GGWCC 2 cut(s) 1087, 2854
VspI ATTAAT 2 cut(s) 1230, 2727
XapI RAATTY 4 cut(s) 286, 1658, 1958, 2930
XbaI TCTAGA 2 cut(s) 31, 2621
XceI RCATGY 6 cut(s) 219, 777, 789, 1060, 2212, 2532
XspI CTAG 5 cut(s) 32, 317, 342, 2622, 2873
Zsp2I ATGCAT 3 cut(s) 217, 2534, 3049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.