MD00G1187500.v1.1

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005367
Physical Location & Seq
Forward (+)
44446100 .. 44455115
9016 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1187500.v1.1.491

Sequence Viewer

Length: 3054 bp
ATGGGGTCGGATATGCAGTCTGTTGGTTTTCTAGACACTTTAAAAATGGAGAGGGTTAGGACAATTTTAACCCATACATATCCTTATCCGCATGAACATTCCCGTCATGCCGTAATTGCTGTTGTTGTGGGTTGCCTCTTTTTTATCTCTTCTGACAACATCAACACCCTTATAGAAAAGTTAGACAACAATATTAAGTGGTGGTCTATGTATGCATGCTTGTTCGGTTTCTTCTATTTCTTTTCGTCCCCATTTATAGGGAAGACTATCAAACCGAGTTATTCAAATTTCAGTCGGTGGTATATAGTCTGGATTTTAGTGGCGGCTGTCTATCATCTTCCTAGTTTTCAATCAATGGGAGTTGATATGAGGATGAATCTGTCTTTGGGTTTTAATGTATTTGTCACTTCTGTTCTGTTTCTTCTTTTCTTCCACATTATATTCATTGGTCTTTGGTACATTGGTCTCGTGTCACGTGTTGCTGGAAGGAGGCCAGCGATTTTTGACTATTCTTCAAAATTGTGCTCCTGCTGTGTGTTTTTTTATAGTCATTGTGGCAACCGTGCTATTCTGAGAGATAGACAACTGGTACGAAGAAACTCTTGGTTTAATTTTTGGAAGAAAGATGAGAGGAACACATGGCTTTCAAAATTCCTCCGAATGAATGAGTTGAAAGATCAGGTCTGCTCATCTTGGTTTGCTCCGGTTGGGTCTGCAAGTGATTATCCACTTTTGTCCAAATGGGTCATTTATGGTGAGTTAGCATGCAATGGTGCGTGTGCTGGTCCATCTGATGAAATCTCCCCATTGTATTCATTATGGGCCACATTCATAGGCCTCTACATTGCTAATTATGTAGTGGAGAGATCAACAGGGTGGGCTCTTACTCACCCTCTGTCTGTTGAAGAACATCAGAAGTCGAAGGAGAAGCAAATGAAGCCACATTTTTTGGATATGGTTCCTTGGTATTCGGGAACATCAGCTGATTTATTCAAGACTGTCTTTGACCTCTTGGTATCAGTAACTGTCTTTGTTGGCCGCTTTGACATGCGTATGATGCAGGCAGCAATGGACAAGGTCCATGATGGAGCTGAACAAAAGGATCTTTTATATGATAACTTTGTTGAGAAGGATGATCTATGGTTTGACTTCATGGCTGATACGGGCGATGGTGGAAACTCCTCTTATTCTGTGGCTCGGCTCCTTGCTCAACCTTCCATTAATATTAACAGAGATGGTTCCATGCTGAAGTTACCACGTGGAGACTTGCTACTTATTGGAGGGGATCTTGCGTATCCTAATCCATCAGCGTTCACCTATGAAAGGCGCCTTTTTTGTCCTTTTGAGTATGCTCTCCAGCCTCCTCCATGGTCTAAACAAGAGCATATAGCTGTAGATAAGCCTGAACTACCTCGTGGTGTGTCTGAACTGAAGAACTATGATGGACCTCAGTGTTTTGTCATTCCTGGAAACCATGATTGGTTTGATGGACTTCATACATTCATGAGGTATATATGCCATAAGAGCTGGTTGGGTGGGTGGTTTATGCCTCAAAAGAAGAGTTATTTCGCTCTGCAACTCCCTAAAAGATGGTGGGTATTTGGTTTTGATCTCGCACTCCACGGTGATATTGATGTGTACCAATTCAAATTCTTTACAGAACTTATTAAGAACAAGGTCAGAGATGGTGACTCTGTAATCATTATGACACATGAACCAAATTGGCTTCTTGATTGGTACTGGAACGATGTTTCTGGAAAGAATGTTGCGCACCTGATATGCGATTATTTAAAAGGGAGATGTAAGCTTCGAGTGGCTGGAGATTTGCATCATTATATGCGTCATTCATTTATTAAAACAAATGGTCCAGTTCCCGTGCAACATTTAATCGTAAATGGTTGTGGTGGGGCTTTTCTACATCCCACTCACACCTTTAGTAATTTTAAGAAATTTTGTGGAGTTTCATATGAGACCAAGGCTGCTTATCCTTCATTTGAAGATTCAAGCAGGATTGCTCTGGGAAATATTCTAAAGTTTCGAAAGAAGAACTGGCAATTTGATTTTATCGGTGGCATTATATACTTCATATTGGTCTTTTCTATGTTCCCACAGTGTAAGCTTGGTCATATCCTACGAGAAGATTCATTTTCTGGTCACGTGGGGTCTTTCTTTCAAACTGTGTGGAATGCTTTTATGTACATGCTGGGACGCTCTTATGTGTCTTTAGCAGGTGCTGTTCTGCTGTTAATTGTGGCAGTTACATTTGTCCCCTCTAAAGTGTCTCGGAAGAAACGATTGATGATTGGAGTTCTTCATGTGTCTGCGCACCTGGCTGCAGCTCTAATACTTATGCTACTGTTGGAACTAGGTGTTGAGATGTGTGTCCAGCATAAACTACTGGGAACTTCAGGCTATCATACTTTATATCAGTGGTACCGATCGGTGGAGAGTGAGCACTTTCCAGATCCAACTGGCCTTCGAGCTCGTATAGAACAATGGACTTTTGGCCTTTATCCAGCATGCATCAAGTATTTCATGTCTGCATTTGATGTTCCAGAGGTTATGGCTGTCACCCGAAACAATATATGCAAGAATGGGATGGAATCGCTATCTAGAGCGGGTGCTGTTATATATTATGCTTCCGTCTTCCTTTATTTCTGGGTCTTCTCGACTCCAGTGGTTTCTTTGGTGTTCGGAAGTTACTTATACATCTGCATTAATTGGCTCCACATACACTTCGATGAAGCCTTCTCCTCTCTCCGAATTGCTAATTACAAGTCTTTCACCCGATTCCACATCAACAGTGACGGTGATCTTGAAGTTTACACTCTCGCAGTTGATAGGGTCCCGAAAGCATGGAAGCTAGACCCTGAATGGGATCGGGAGCCAAAACAGCCGGAACAAATGAGCCACCTTAGAAAATTTCCCAGCAAATGGAGTGCAGCAGCCGGTCAACAAGATCCGTTACATACTGTCAAAATCGTTGATCAGTTTGTTATTCGACAAACAGATAAAACTGATGTTGGAGCAAGTGATGGATCAGTAATTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

1018

Amino Acids

116.98

Weight (kDa)

8.62

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 384 - 585 4.5e-06 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2219
Acc16I TGCGCA 2 cut(s) 1770, 2325
Acc36I ACCTGC 1 cut(s) 2219
Acc65I GGTACC 1 cut(s) 2433
AccB1I GGYRCC 2 cut(s) 1326, 2433
AccB7I CCANNNNNTGG 1 cut(s) 2934
AccBSI CCGCTC 1 cut(s) 2618
AciI CCGC 4 cut(s) 89, 323, 1039, 2618
AclWI GGATC 6 cut(s) 1110, 1293, 2459, 2886, 2954, 3046
AcoI YGGCCR 1 cut(s) 1036
AcsI RAATTY 5 cut(s) 286, 650, 1649, 1949, 2921
AcuI CTGAAG 3 cut(s) 1268, 1451, 2391
AcvI CACGTG 3 cut(s) 476, 1259, 2158
AcyI GRCGYC 1 cut(s) 1327
AfaI GTAC 6 cut(s) 458, 591, 1640, 1739, 2198, 2435
AfiI CCNNNNNNNGG 5 cut(s) 257, 1323, 2443, 2875, 2934
AflIII ACRYGT 1 cut(s) 475
AjnI CCWGG 2 cut(s) 1465, 2328
AjuI GAANNNNNNNTTGG 6 cut(s) 368, 400, 586, 618, 1462, 1494
Alw21I GWGCWC 3 cut(s) 527, 2457, 2485
Alw26I GTCTC 4 cut(s) 470, 1257, 1964, 2286
AlwI GGATC 6 cut(s) 1110, 1293, 2459, 2886, 2954, 3046
AlwNI CAGNNNCTG 2 cut(s) 1025, 2234
AoxI GGCC 6 cut(s) 491, 822, 835, 1036, 2473, 2506
ApeKI GCWGC 6 cut(s) 1064, 1979, 2333, 2336, 2942, 2945
ApoI RAATTY 5 cut(s) 286, 650, 1649, 1949, 2921
AseI ATTAAT 2 cut(s) 1221, 2718
Asp718I GGTACC 1 cut(s) 2433
AspLEI GCGC 3 cut(s) 1329, 1771, 2326
AspS9I GGNCC 6 cut(s) 785, 822, 1078, 1445, 1865, 2845
AsuHPI GGTGA 8 cut(s) 767, 881, 1306, 1637, 1700, 2563, 2776, 2822
AsuII TTCGAA 1 cut(s) 2038
AvaII GGWCC 5 cut(s) 785, 1078, 1445, 1865, 2845
BaeI ACNNNNGTAYC 2 cut(s) 448, 481
BanI GGYRCC 2 cut(s) 1326, 2433
BanII GRGCYC 2 cut(s) 883, 2485
BauI CACGAG 2 cut(s) 467, 1413
BbrPI CACGTG 3 cut(s) 476, 1259, 2158
BbsI GAAGAC 3 cut(s) 269, 2638, 2656
Bbv12I GWGCWC 3 cut(s) 527, 2457, 2485
BbvI GCAGC 6 cut(s) 1076, 1966, 2320, 2348, 2954, 2957
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 2 cut(s) 1467, 2330
BciVI GTATCC 1 cut(s) 1305
BclI TGATCA 1 cut(s) 2986
BcoDI GTCTC 4 cut(s) 470, 1257, 1964, 2286
BfaI CTAG 5 cut(s) 32, 342, 2366, 2613, 2864
BfmI CTRYAG 2 cut(s) 1392, 2334
BfoI RGCGCY 1 cut(s) 1330
BfuAI ACCTGC 1 cut(s) 2219
BfuI GTATCC 1 cut(s) 1305
BisI GCNGC 8 cut(s) 324, 1039, 1065, 1980, 2334, 2337, 2943, 2946
BlsI GCNGC 8 cut(s) 325, 1040, 1066, 1981, 2335, 2338, 2944, 2947
Bme1390I CCNGG 2 cut(s) 1467, 2330
Bme18I GGWCC 5 cut(s) 785, 1078, 1445, 1865, 2845
BmgT120I GGNCC 6 cut(s) 785, 822, 1078, 1445, 1865, 2845
BmiI GGNNCC 9 cut(s) 960, 1202, 1240, 1328, 2435, 2726, 2846, 2847, 2886
BmrFI CCNGG 2 cut(s) 1467, 2330
BmrI ACTGGG 1 cut(s) 2408
BmsI GCATC 3 cut(s) 1047, 1837, 2532
BmuI ACTGGG 1 cut(s) 2408
BpiI GAAGAC 3 cut(s) 269, 2638, 2656
BpmI CTGGAG 3 cut(s) 1340, 1839, 2658
Bpu14I TTCGAA 1 cut(s) 2038
BsaAI YACGTR 3 cut(s) 476, 1259, 2158
BsaBI GATNNNNATC 1 cut(s) 1827
BsaHI GRCGYC 1 cut(s) 1327
BsaI GGTCTC 2 cut(s) 470, 1964
BsaJI CCNNGG 4 cut(s) 962, 1367, 1621, 1974
BsaWI WCCGGW 1 cut(s) 703
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 1323, 2443, 2875, 2934
Bse118I RCCGGY 1 cut(s) 2948
Bse1I ACTGG 7 cut(s) 591, 1745, 1868, 2054, 2403, 2476, 2675
Bse3DI GCAATG 3 cut(s) 775, 843, 1074
Bse8I GATNNNNATC 1 cut(s) 1827
BseBI CCWGG 2 cut(s) 1467, 2330
BseDI CCNNGG 4 cut(s) 962, 1367, 1621, 1974
BseGI GGATG 4 cut(s) 378, 1138, 1918, 2604
BseJI GATNNNNATC 1 cut(s) 1827
BseLI CCNNNNNNNGG 5 cut(s) 257, 1323, 2443, 2875, 2934
BseMI GCAATG 3 cut(s) 775, 843, 1074
BseMII CTCAG 2 cut(s) 563, 1463
BseNI ACTGG 7 cut(s) 591, 1745, 1868, 2054, 2403, 2476, 2675
BseRI GAGGAG 3 cut(s) 1171, 1353, 2743
BseXI GCAGC 6 cut(s) 1076, 1966, 2320, 2348, 2954, 2957
BseYI CCCAGC 2 cut(s) 2203, 2927
BsgI GTGCAG 1 cut(s) 2961
Bsh1285I CGRYCG 1 cut(s) 2441
BshFI GGCC 6 cut(s) 493, 824, 837, 1038, 2475, 2508
BshNI GGYRCC 2 cut(s) 1326, 2433
BsiEI CGRYCG 1 cut(s) 2441
BsiHKAI GWGCWC 3 cut(s) 527, 2457, 2485
BsiSI CCGG 3 cut(s) 704, 2897, 2949
BslFI GGGAC 4 cut(s) 232, 2220, 2252, 2831
BslI CCNNNNNNNGG 5 cut(s) 257, 1323, 2443, 2875, 2934
BsmAI GTCTC 4 cut(s) 470, 1257, 1964, 2286
BsmFI GGGAC 4 cut(s) 232, 2220, 2252, 2831
BsmI GAATGC 1 cut(s) 2191
BsnI GGCC 6 cut(s) 493, 824, 837, 1038, 2475, 2508
Bso31I GGTCTC 2 cut(s) 470, 1964
Bsp119I TTCGAA 1 cut(s) 2038
Bsp1286I GDGCHC 4 cut(s) 527, 883, 2457, 2485
Bsp1407I TGTACA 1 cut(s) 2196
Bsp19I CCATGG 1 cut(s) 1367
BspACI CCGC 4 cut(s) 89, 323, 1039, 2618
BspANI GGCC 6 cut(s) 493, 824, 837, 1038, 2475, 2508
BspCNI CTCAG 2 cut(s) 564, 1462
BspHI TCATGA 1 cut(s) 1503
BspLI GGNNCC 9 cut(s) 960, 1202, 1240, 1328, 2435, 2726, 2846, 2847, 2886
BspMAI CTGCAG 1 cut(s) 2338
BspMI ACCTGC 1 cut(s) 2219
BspPI GGATC 6 cut(s) 1110, 1293, 2459, 2886, 2954, 3046
BspT104I TTCGAA 1 cut(s) 2038
BspT107I GGYRCC 2 cut(s) 1326, 2433
BspTNI GGTCTC 2 cut(s) 470, 1964
BsrBI CCGCTC 1 cut(s) 2618
BsrDI GCAATG 3 cut(s) 775, 843, 1074
BsrFI RCCGGY 1 cut(s) 2948
BsrGI TGTACA 1 cut(s) 2196
BsrI ACTGG 7 cut(s) 591, 1745, 1868, 2054, 2403, 2476, 2675
BssAI RCCGGY 1 cut(s) 2948
BssECI CCNNGG 4 cut(s) 962, 1367, 1621, 1974
BssNI GRCGYC 1 cut(s) 1327
BssSI CACGAG 2 cut(s) 467, 1413
BssT1I CCWWGG 3 cut(s) 962, 1367, 1974
Bst2BI CACGAG 2 cut(s) 467, 1413
Bst2UI CCWGG 2 cut(s) 1467, 2330
Bst6I CTCTTC 2 cut(s) 154, 1553
BstACI GRCGYC 1 cut(s) 1327
BstAUI TGTACA 1 cut(s) 2196
BstBAI YACGTR 3 cut(s) 476, 1259, 2158
BstBI TTCGAA 1 cut(s) 2038
BstC8I GCNNGC 5 cut(s) 217, 495, 766, 1062, 2521
BstDEI CTNAG 3 cut(s) 572, 1449, 2915
BstDSI CCRYGG 2 cut(s) 1367, 1621
BstENI CCTNNNNNAGG 1 cut(s) 1321
BstF5I GGATG 4 cut(s) 378, 1138, 1918, 2604
BstH2I RGCGCY 1 cut(s) 1330
BstHHI GCGC 3 cut(s) 1329, 1771, 2326
BstMAI GTCTC 4 cut(s) 470, 1257, 1964, 2286
BstMCI CGRYCG 1 cut(s) 2441
BstMWI GCNNNNNNNGC 6 cut(s) 116, 937, 1057, 1524, 2330, 2893
BstNI CCWGG 2 cut(s) 1467, 2330
BstNSI RCATGY 5 cut(s) 219, 768, 1051, 2203, 2523
BstSCI CCNGG 2 cut(s) 1465, 2328
BstSFI CTRYAG 2 cut(s) 1392, 2334
BstV1I GCAGC 6 cut(s) 1076, 1966, 2320, 2348, 2954, 2957
BstV2I GAAGAC 3 cut(s) 269, 2638, 2656
BstX2I RGATCY 4 cut(s) 1102, 1285, 2464, 2959
BstYI RGATCY 4 cut(s) 1102, 1285, 2464, 2959
BsuI GTATCC 1 cut(s) 1305
BsuRI GGCC 6 cut(s) 493, 824, 837, 1038, 2475, 2508
BtgI CCRYGG 2 cut(s) 1367, 1621
BtgZI GCGATG 1 cut(s) 1182
BtsCI GGATG 4 cut(s) 378, 1138, 1918, 2604
BtsIMutI CAGTG 5 cut(s) 1457, 2117, 2435, 2682, 2809
BveI ACCTGC 1 cut(s) 2219
Cac8I GCNNGC 5 cut(s) 217, 495, 766, 1062, 2521
CaiI CAGNNNCTG 2 cut(s) 1025, 2234
CciI TCATGA 1 cut(s) 1503
CfoI GCGC 3 cut(s) 1329, 1771, 2326
Cfr10I RCCGGY 1 cut(s) 2948
Cfr13I GGNCC 6 cut(s) 785, 822, 1078, 1445, 1865, 2845
CseI GACGC 2 cut(s) 1829, 2217
Csp6I GTAC 6 cut(s) 457, 590, 1639, 1738, 2197, 2434
CspCI CAANNNNNGTGG 4 cut(s) 930, 965, 2162, 2197
CviQI GTAC 6 cut(s) 457, 590, 1639, 1738, 2197, 2434
DdeI CTNAG 3 cut(s) 572, 1449, 2915
DinI GGCGCC 1 cut(s) 1328
DraI TTTAAA 2 cut(s) 42, 1791
EaeI YGGCCR 1 cut(s) 1036
Eam1104I CTCTTC 2 cut(s) 154, 1553
EarI CTCTTC 2 cut(s) 154, 1553
Ecl136II GAGCTC 1 cut(s) 2483
Eco130I CCWWGG 3 cut(s) 962, 1367, 1974
Eco147I AGGCCT 1 cut(s) 837
Eco24I GRGCYC 2 cut(s) 883, 2485
Eco31I GGTCTC 2 cut(s) 470, 1964
Eco47I GGWCC 5 cut(s) 785, 1078, 1445, 1865, 2845
Eco53kI GAGCTC 1 cut(s) 2483
Eco57I CTGAAG 3 cut(s) 1268, 1451, 2391
Eco72I CACGTG 3 cut(s) 476, 1259, 2158
EcoICRI GAGCTC 1 cut(s) 2483
EcoNI CCTNNNNNAGG 1 cut(s) 1321
EcoO109I RGGNCCY 1 cut(s) 2845
EcoRII CCWGG 2 cut(s) 1465, 2328
EcoT14I CCWWGG 3 cut(s) 962, 1367, 1974
EcoT22I ATGCAT 2 cut(s) 217, 2525
EcoT38I GRGCYC 2 cut(s) 883, 2485
EgeI GGCGCC 1 cut(s) 1328
EheI GGCGCC 1 cut(s) 1328
ErhI CCWWGG 3 cut(s) 962, 1367, 1974
FalI AAGNNNNNCTT 4 cut(s) 586, 618, 986, 1018
FaqI GGGAC 4 cut(s) 232, 2220, 2252, 2831
FauI CCCGC 1 cut(s) 2611
FauNDI CATATG 1 cut(s) 1966
FbaI TGATCA 1 cut(s) 2986
Fnu4HI GCNGC 8 cut(s) 324, 1039, 1065, 1980, 2334, 2337, 2943, 2946
FokI GGATG 4 cut(s) 385, 1145, 1905, 2611
FriOI GRGCYC 2 cut(s) 883, 2485
Fsp4HI GCNGC 8 cut(s) 324, 1039, 1065, 1980, 2334, 2337, 2943, 2946
FspBI CTAG 5 cut(s) 32, 342, 2366, 2613, 2864
FspI TGCGCA 2 cut(s) 1770, 2325
GlaI GCGC 3 cut(s) 1328, 1770, 2325
GluI GCNGC 8 cut(s) 324, 1039, 1065, 1980, 2334, 2337, 2943, 2946
GsaI CCCAGC 2 cut(s) 2207, 2931
GsuI CTGGAG 3 cut(s) 1340, 1839, 2658
HaeII RGCGCY 1 cut(s) 1330
HaeIII GGCC 6 cut(s) 493, 824, 837, 1038, 2475, 2508
HapII CCGG 3 cut(s) 704, 2897, 2949
HgaI GACGC 2 cut(s) 1829, 2217
HhaI GCGC 3 cut(s) 1329, 1771, 2326
Hin1I GRCGYC 1 cut(s) 1327
Hin6I GCGC 3 cut(s) 1327, 1769, 2324
HinP1I GCGC 3 cut(s) 1327, 1769, 2324
HincII GTYRAC 1 cut(s) 2954
HindII GTYRAC 1 cut(s) 2954
HindIII AAGCTT 2 cut(s) 1805, 2117
HinfI GANTC 7 cut(s) 376, 1691, 2000, 2141, 2603, 2671, 2790
HpaII CCGG 3 cut(s) 704, 2897, 2949
HphI GGTGA 8 cut(s) 767, 881, 1306, 1637, 1700, 2563, 2776, 2822
Hpy166II GTNNAC 4 cut(s) 1314, 1639, 2824, 2954
Hpy8I GTNNAC 4 cut(s) 1314, 1639, 2824, 2954
HpyAV CCTTC 7 cut(s) 480, 916, 1123, 1224, 1998, 2486, 2758
HpyCH4IV ACGT 3 cut(s) 475, 1258, 2157
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 937, 1057, 1524, 2330, 2893
HpyF3I CTNAG 3 cut(s) 572, 1449, 2915
HpySE526I ACGT 3 cut(s) 475, 1258, 2157
Hsp92I GRCGYC 1 cut(s) 1327
HspAI GCGC 3 cut(s) 1327, 1769, 2324
KasI GGCGCC 1 cut(s) 1326
KflI GGGWCCC 1 cut(s) 2845
KpnI GGTACC 1 cut(s) 2437
Ksp22I TGATCA 1 cut(s) 2986
LmnI GCTCC 7 cut(s) 530, 706, 1088, 1206, 2730, 2884, 3026
Lsp1109I GCAGC 6 cut(s) 1076, 1966, 2320, 2348, 2954, 2957
LweI GCATC 3 cut(s) 1047, 1837, 2532
MaeI CTAG 5 cut(s) 32, 342, 2366, 2613, 2864
MaeII ACGT 3 cut(s) 475, 1258, 2157
MbiI CCGCTC 1 cut(s) 2618
MflI RGATCY 4 cut(s) 1102, 1285, 2464, 2959
MhlI GDGCHC 4 cut(s) 527, 883, 2457, 2485
Mly113I GGCGCC 1 cut(s) 1327
MlyI GAGTC 2 cut(s) 1685, 2665
MmeI TCCRAC 3 cut(s) 2340, 2492, 3004
Mph1103I ATGCAT 2 cut(s) 217, 2525
MslI CAYNNNNRTG 2 cut(s) 1052, 2739
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 3 cut(s) 704, 2897, 2949
MspR9I CCNGG 2 cut(s) 1467, 2330
Mva1269I GAATGC 1 cut(s) 2191
MvaI CCWGG 2 cut(s) 1467, 2330
MwoI GCNNNNNNNGC 6 cut(s) 116, 937, 1057, 1524, 2330, 2893
NarI GGCGCC 1 cut(s) 1327
NcoI CCATGG 1 cut(s) 1367
NdeI CATATG 1 cut(s) 1966
NlaIV GGNNCC 9 cut(s) 960, 1202, 1240, 1328, 2435, 2726, 2846, 2847, 2886
NmeAIII GCCGAG 1 cut(s) 1177
NmuCI GTSAC 6 cut(s) 403, 471, 1688, 2153, 2569, 2804
NsbI TGCGCA 2 cut(s) 1770, 2325
NsiI ATGCAT 2 cut(s) 217, 2525
NspI RCATGY 5 cut(s) 219, 768, 1051, 2203, 2523
NspV TTCGAA 1 cut(s) 2038
PaeI GCATGC 3 cut(s) 219, 768, 2523
PagI TCATGA 1 cut(s) 1503
PaqCI CACCTGC 1 cut(s) 2219
PceI AGGCCT 1 cut(s) 837
PcsI WCGNNNNNNNCGW 1 cut(s) 2290
PctI GAATGC 1 cut(s) 2191
PfeI GAWTC 5 cut(s) 376, 2000, 2141, 2603, 2790
PflFI GACNNNGTC 1 cut(s) 1076
PflMI CCANNNNNTGG 1 cut(s) 2934
PfoI TCCNGGA 1 cut(s) 1465
PkrI GCNGC 8 cut(s) 325, 1040, 1066, 1981, 2335, 2338, 2944, 2947
Ple19I CGATCG 1 cut(s) 2441
PleI GAGTC 2 cut(s) 1685, 2665
PluTI GGCGCC 1 cut(s) 1330
PmaCI CACGTG 3 cut(s) 476, 1259, 2158
PmlI CACGTG 3 cut(s) 476, 1259, 2158
PpsI GAGTC 2 cut(s) 1685, 2665
Ppu21I YACGTR 3 cut(s) 476, 1259, 2158
PpuMI RGGWCCY 1 cut(s) 2845
PshBI ATTAAT 2 cut(s) 1221, 2718
Psp124BI GAGCTC 1 cut(s) 2485
Psp5II RGGWCCY 1 cut(s) 2845
Psp6I CCWGG 2 cut(s) 1465, 2328
PspCI CACGTG 3 cut(s) 476, 1259, 2158
PspFI CCCAGC 2 cut(s) 2203, 2927
PspGI CCWGG 2 cut(s) 1465, 2328
PspN4I GGNNCC 9 cut(s) 960, 1202, 1240, 1328, 2435, 2726, 2846, 2847, 2886
PspPI GGNCC 6 cut(s) 785, 822, 1078, 1445, 1865, 2845
PspPPI RGGWCCY 1 cut(s) 2845
PstI CTGCAG 1 cut(s) 2338
PstNI CAGNNNCTG 2 cut(s) 1025, 2234
PsuI RGATCY 4 cut(s) 1102, 1285, 2464, 2959
PsyI GACNNNGTC 1 cut(s) 1076
PvuI CGATCG 1 cut(s) 2441
PvuII CAGCTG 1 cut(s) 983
RsaI GTAC 6 cut(s) 458, 591, 1640, 1739, 2198, 2435
RsaNI GTAC 6 cut(s) 457, 590, 1639, 1738, 2197, 2434
RseI CAYNNNNRTG 2 cut(s) 1052, 2739
SacI GAGCTC 1 cut(s) 2485
SatI GCNGC 8 cut(s) 324, 1039, 1065, 1980, 2334, 2337, 2943, 2946
Sau96I GGNCC 6 cut(s) 785, 822, 1078, 1445, 1865, 2845
SchI GAGTC 2 cut(s) 1685, 2665
ScrFI CCNGG 2 cut(s) 1467, 2330
SduI GDGCHC 4 cut(s) 527, 883, 2457, 2485
SfaNI GCATC 3 cut(s) 1047, 1837, 2532
SfcI CTRYAG 2 cut(s) 1392, 2334
SfoI GGCGCC 1 cut(s) 1328
SfuI TTCGAA 1 cut(s) 2038
SinI GGWCC 5 cut(s) 785, 1078, 1445, 1865, 2845
SmiMI CAYNNNNRTG 2 cut(s) 1052, 2739
SphI GCATGC 3 cut(s) 219, 768, 2523
SseBI AGGCCT 1 cut(s) 837
SsiI CCGC 4 cut(s) 89, 323, 1039, 2618
SspDI GGCGCC 1 cut(s) 1326
SspI AATATT 3 cut(s) 193, 1225, 2026
SspMI CTAG 5 cut(s) 32, 342, 2366, 2613, 2864
SstI GAGCTC 1 cut(s) 2485
StuI AGGCCT 1 cut(s) 837
StyD4I CCNGG 2 cut(s) 1465, 2328
StyI CCWWGG 3 cut(s) 962, 1367, 1974
TaiI ACGT 3 cut(s) 478, 1261, 2160
TaqI TCGA 7 cut(s) 920, 1810, 2038, 2479, 2669, 2739, 3001
TatI WGTACW 1 cut(s) 2196
TauI GCSGC 2 cut(s) 326, 1041
TfiI GAWTC 5 cut(s) 376, 2000, 2141, 2603, 2790
TscAI CASTG 5 cut(s) 1457, 2117, 2435, 2682, 2809
TseFI GTSAC 6 cut(s) 403, 471, 1688, 2153, 2569, 2804
TseI GCWGC 6 cut(s) 1064, 1979, 2333, 2336, 2942, 2945
Tsp45I GTSAC 6 cut(s) 403, 471, 1688, 2153, 2569, 2804
TspGWI ACGGA 2 cut(s) 2632, 2952
TspRI CASTG 5 cut(s) 1457, 2117, 2435, 2682, 2809
Tth111I GACNNNGTC 1 cut(s) 1076
Van91I CCANNNNNTGG 1 cut(s) 2934
VpaK11BI GGWCC 5 cut(s) 785, 1078, 1445, 1865, 2845
VspI ATTAAT 2 cut(s) 1221, 2718
XagI CCTNNNNNAGG 1 cut(s) 1321
XapI RAATTY 5 cut(s) 286, 650, 1649, 1949, 2921
XbaI TCTAGA 2 cut(s) 31, 2612
XceI RCATGY 5 cut(s) 219, 768, 1051, 2203, 2523
XspI CTAG 5 cut(s) 32, 342, 2366, 2613, 2864
Zsp2I ATGCAT 2 cut(s) 217, 2525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.