Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0014514

Physical Location & Seq

Genomic Coordinates Forward (+)

26609929 .. 26611410

Gene Structure 1482 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g36320.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGGTGGATGTGTGCGACTACAGTGGCAATGTGAACTATGCTAGCTTGCTATTCAAGCAAGTTCTTGAGCCAAATGTGTTCCTTTTCAATGCGATGATCCGAGCTTATACGCATAACTATGTCTATGATTCAGCAATAGCTTTGTATAAGCAAATGCTGAGACACCCAGAATCTGAAACCCTAATTTTCCCAGACAAATTCACATTCCCATTTGTGATAAAATGTTGTGCAGGGCTAGGTTGTGAAGATTTGGGGAGGCAGGTTCATGGTCAAGTGTGGAAATTTGGGCCTGGGTCGCATTTGCTGATTGAGAATGCATTGATGGCTTTTTATACCAAGTGTGATAATTTGAGGGATGCACATAAGGTGTTTGATGAAATGACTGAGAGAGATGTGATTTCTTGGAATAGTCTTCTTAGTGGTTATGTTAGGTTGGGGCAGATGACGAAAGCGAGAGCAGTGTTTGAGGAGATGAGTGATAAGAGTATTGTGTCTTGGACGGCGATGGTTTCGGGGTACACTCGGATCGGGTGTTACGGGGATGCTTTGGAGATTTTTCGACGAATGCAGATGCTGGGTATCGAACCTGATGAGATCAGTGTTGTGTCGGTTTTGCCGGCGTGCTCGCAGCTTGGAGCTCTTGAGGTTGGGAAGTGGATTCACAGGTACTCGGATAAGAAGCGACTGCTACAGTCGGTCTCGGTGTGCAATGCTTTGATTGAAATGTATGTGAAATGTGGCTGCATCGATCAAGCTTGGCAGTTGTTTGATCAGATGTCGGGGAGGGATGTGATATCTTGGAGTACCATGATTGCAGGGCTGGCGAATAATGGTAAAGCTCACGAAGCAGTTGAGCTGTTTCAGGAGATGCAGAGAGTGAAGACTGAACCTAATGGGATTACATTTCTTGGTGTTTTATCAGCCTGCAGCCATGCTGGTTTATGGAAGGAGGGACTCAAGTATTTTGACTCTATGAGGGAGGGGTGTACGATTGAGCCAGGAATTGAGCATTATGGTTGTTTAGTTGATCTTCTGGGACGTGCTGGACACCTTGATCAGGCTCTCGACAAGATAAAGCAGATGCCAATGAAGCCAGACTCAAAGATTTGGGGCTCCTTGTTAAGCTCTTGCAGGACTTTTTGCAATCTTGAGATTGCTCTCATCGCAATGGAGCAACTTCAAGATCTTGAACCGGATGATGCAGGGAACTATGTGTTACTTTCCAATATATATGCAGACCTGGGGAAGTGGGAGGATGTGTCCAGGATGAGGAAACTGATACGAAGCAAAAGCATGAAGAAAACCCCAGGTTGCAGTTTGATTGAGGTTAACAATGTAGTTTTAGAATTCGTATCAGGGGATGACACAAAACCATTTGCAAAAGACATCTTTTCTATGCTACAGCTGCTGGTTGCACACCAAAGCATGCCAGGTGAGAGCATTGAAGCTGTGCCAGAGGACTGCAGTCAAATACTGTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0080156 GO:0090304 GO:0090305 GO:0097159 GO:1900864 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

494

Amino Acids

55.4

Weight (kDa)

5.41

Isoelectric Point (pI)

44.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	130 - 163	3.4e-07	PPR repeat family
PPR	PF01535	133 - 162	2.6e-07	PPR repeat
PPR_2	PF13041	162 - 208	7.1e-07	PPR repeat family
PPR	PF01535	164 - 194	7.8e-07	PPR repeat
PPR_2	PF13041	232 - 262	6e-06	PPR repeat family
PPR_2	PF13041	262 - 310	8.9e-11	PPR repeat family
PPR_1	PF12854	263 - 291	2e-06	PPR repeat
PPR	PF01535	265 - 292	2.1e-07	PPR repeat
E_motif	PF20431	381 - 442	1.4e-22	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014514)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20540 AT2G20540
fragaria_vesca	FvH4_2g36320
malus_domestica	MD15G1099800.v1.1
prunus_persica	Prupe.1G454600_v2.0.a1
pyrus_communis	pycom15g09180
rosa_chinensis	RchiOBHm_Chr6g0305561
rosa_laevigata	RLG00000010877
rosa_multiflora	Rmu_co8430547.1_g000001 Rmu_sc0000841.1_g000002 Rmu_sc0001153.1_g000005
rosa_roxburghii	Rroxscaffold_7G00162590
rosa_rugosa	Rorug06G0341700
rosa_samantha	Rh6AG453700 Rh6BG447900 Rh6DG454000
rosa_wichuraiana	Rw6G039540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	250
AclWI	GGATC	2 cut(s)	91, 533
AcsI	RAATTY	3 cut(s)	197, 281, 1346
AfaI	GTAC	4 cut(s)	518, 668, 805, 988
AfiI	CCNNNNNNNGG	2 cut(s)	1057, 1269
AgsI	TTSAA	6 cut(s)	55, 88, 722, 1181, 1190, 1445
AjiI	CACGTC	1 cut(s)	1040
AjnI	CCWGG	6 cut(s)	289, 997, 1239, 1262, 1306, 1429
Alw21I	GWGCWC	2 cut(s)	626, 640
Alw26I	GTCTC	2 cut(s)	154, 703
AlwI	GGATC	2 cut(s)	91, 533
AlwNI	CAGNNNCTG	3 cut(s)	173, 574, 1408
AoxI	GGCC	1 cut(s)	287
ApeKI	GCWGC	4 cut(s)	628, 741, 927, 1405
ApoI	RAATTY	3 cut(s)	197, 281, 1346
AspS9I	GGNCC	1 cut(s)	287
AsuHPI	GGTGA	1 cut(s)	1445
AsuNHI	GCTAGC	1 cut(s)	41
BanII	GRGCYC	2 cut(s)	640, 1115
BbsI	GAAGAC	2 cut(s)	404, 887
Bbv12I	GWGCWC	2 cut(s)	626, 640
BbvI	GCAGC	4 cut(s)	640, 728, 939, 1392
BccI	CCATC	2 cut(s)	316, 499
BceAI	ACGGC	1 cut(s)	516
BciT130I	CCWGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
BclI	TGATCA	2 cut(s)	769, 1054
BcoDI	GTCTC	2 cut(s)	154, 703
BfaI	CTAG	2 cut(s)	42, 236
BfmI	CTRYAG	5 cut(s)	19, 689, 925, 1400, 1462
BfuAI	ACCTGC	1 cut(s)	250
BglII	AGATCT	1 cut(s)	1183
BisI	GCNGC	4 cut(s)	629, 742, 928, 1406
BlsI	GCNGC	4 cut(s)	630, 743, 929, 1407
Bme1390I	CCNGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
BmgBI	CACGTC	1 cut(s)	1040
BmgT120I	GGNCC	1 cut(s)	287
BmiI	GGNNCC	1 cut(s)	1114
BmrFI	CCNGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
BmsI	GCATC	7 cut(s)	346, 532, 561, 753, 858, 1071, 1189
BmtI	GCTAGC	1 cut(s)	45
BoxI	GACNNNNGTC	1 cut(s)	1464
BpiI	GAAGAC	2 cut(s)	404, 887
BpuEI	CTTGAG	4 cut(s)	86, 662, 941, 1169
Bsa29I	ATCGAT	1 cut(s)	747
BsaI	GGTCTC	1 cut(s)	703
BsaJI	CCNNGG	3 cut(s)	290, 1240, 1306
BsaWI	WCCGGW	1 cut(s)	1192
BsaXI	ACNNNNNCTCC	2 cut(s)	1244, 1274
Bsc4I	CCNNNNNNNGG	2 cut(s)	1057, 1269
Bse118I	RCCGGY	1 cut(s)	616
Bse3DI	GCAATG	3 cut(s)	34, 715, 1173
BseBI	CCWGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
BseCI	ATCGAT	1 cut(s)	747
BseDI	CCNNGG	3 cut(s)	290, 1240, 1306
BseGI	GGATG	8 cut(s)	13, 361, 547, 793, 1201, 1261, 1272, 1366
BseLI	CCNNNNNNNGG	2 cut(s)	1057, 1269
BseMI	GCAATG	3 cut(s)	34, 715, 1173
BseMII	CTCAG	2 cut(s)	149, 375
BseRI	GAGGAG	1 cut(s)	482
BseXI	GCAGC	4 cut(s)	640, 728, 939, 1392
BseYI	CCCAGC	1 cut(s)	574
BsgI	GTGCAG	1 cut(s)	249
BshFI	GGCC	1 cut(s)	289
BshVI	ATCGAT	1 cut(s)	747
BsiHKAI	GWGCWC	2 cut(s)	626, 640
BsiSI	CCGG	2 cut(s)	617, 1193
BslFI	GGGAC	2 cut(s)	966, 1050
BslI	CCNNNNNNNGG	2 cut(s)	1057, 1269
BsmAI	GTCTC	2 cut(s)	154, 703
BsmFI	GGGAC	2 cut(s)	966, 1050
BsmI	GAATGC	2 cut(s)	319, 570
BsnI	GGCC	1 cut(s)	289
Bso31I	GGTCTC	1 cut(s)	703
Bsp1286I	GDGCHC	3 cut(s)	626, 640, 1115
Bsp143I	GATC	8 cut(s)	96, 525, 594, 748, 769, 1027, 1054, 1183
BspANI	GGCC	1 cut(s)	289
BspCNI	CTCAG	2 cut(s)	150, 376
BspDI	ATCGAT	1 cut(s)	747
BspLI	GGNNCC	1 cut(s)	1114
BspMAI	CTGCAG	2 cut(s)	929, 1466
BspMI	ACCTGC	1 cut(s)	250
BspOI	GCTAGC	1 cut(s)	45
BspPI	GGATC	2 cut(s)	91, 533
BspTNI	GGTCTC	1 cut(s)	703
BsrDI	GCAATG	3 cut(s)	34, 715, 1173
BsrFI	RCCGGY	1 cut(s)	616
BssAI	RCCGGY	1 cut(s)	616
BssECI	CCNNGG	3 cut(s)	290, 1240, 1306
BssMI	GATC	8 cut(s)	96, 525, 594, 748, 769, 1027, 1054, 1183
Bst2UI	CCWGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
Bst4CI	ACNGT	3 cut(s)	23, 693, 1476
BstC8I	GCNNGC	8 cut(s)	43, 47, 618, 622, 626, 822, 925, 1427
BstDEI	CTNAG	3 cut(s)	158, 384, 416
BstENI	CCTNNNNNAGG	1 cut(s)	1055
BstF5I	GGATG	8 cut(s)	13, 361, 547, 793, 1201, 1261, 1272, 1366
BstKTI	GATC	8 cut(s)	99, 528, 597, 751, 772, 1030, 1057, 1186
BstMAI	GTCTC	2 cut(s)	154, 703
BstMBI	GATC	8 cut(s)	96, 525, 594, 748, 769, 1027, 1054, 1183
BstMWI	GCNNNNNNNGC	8 cut(s)	55, 295, 323, 821, 845, 1090, 1163, 1405
BstNI	CCWGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
BstNSI	RCATGY	1 cut(s)	1429
BstPAI	GACNNNNGTC	1 cut(s)	1464
BstSCI	CCNGG	6 cut(s)	289, 997, 1239, 1262, 1306, 1429
BstSFI	CTRYAG	5 cut(s)	19, 689, 925, 1400, 1462
BstV1I	GCAGC	4 cut(s)	640, 728, 939, 1392
BstV2I	GAAGAC	2 cut(s)	404, 887
BstX2I	RGATCY	1 cut(s)	1183
BstYI	RGATCY	1 cut(s)	1183
Bsu15I	ATCGAT	1 cut(s)	747
BsuRI	GGCC	1 cut(s)	289
BsuTUI	ATCGAT	1 cut(s)	747
BtgZI	GCGATG	3 cut(s)	107, 518, 1147
BtrI	CACGTC	1 cut(s)	1040
BtsCI	GGATG	8 cut(s)	13, 361, 547, 793, 1201, 1261, 1272, 1366
BtsI	GCAGTG	1 cut(s)	465
BtsIMutI	CAGTG	3 cut(s)	28, 465, 604
BveI	ACCTGC	1 cut(s)	250
Cac8I	GCNNGC	8 cut(s)	43, 47, 618, 622, 626, 822, 925, 1427
CaiI	CAGNNNCTG	3 cut(s)	173, 574, 1408
Cfr10I	RCCGGY	1 cut(s)	616
Cfr13I	GGNCC	1 cut(s)	287
ClaI	ATCGAT	1 cut(s)	747
Csp6I	GTAC	4 cut(s)	517, 667, 804, 987
CviAII	CATG	5 cut(s)	266, 808, 932, 1294, 1426
CviQI	GTAC	4 cut(s)	517, 667, 804, 987
DdeI	CTNAG	3 cut(s)	158, 384, 416
DpnI	GATC	8 cut(s)	98, 527, 596, 750, 771, 1029, 1056, 1185
DpnII	GATC	8 cut(s)	96, 525, 594, 748, 769, 1027, 1054, 1183
Ecl136II	GAGCTC	1 cut(s)	638
Eco24I	GRGCYC	2 cut(s)	640, 1115
Eco31I	GGTCTC	1 cut(s)	703
Eco32I	GATATC	1 cut(s)	795
Eco53kI	GAGCTC	1 cut(s)	638
EcoICRI	GAGCTC	1 cut(s)	638
EcoNI	CCTNNNNNAGG	1 cut(s)	1055
EcoRI	GAATTC	1 cut(s)	1346
EcoRII	CCWGG	6 cut(s)	289, 997, 1239, 1262, 1306, 1429
EcoRV	GATATC	1 cut(s)	795
EcoT22I	ATGCAT	1 cut(s)	319
EcoT38I	GRGCYC	2 cut(s)	640, 1115
FaeI	CATG	5 cut(s)	269, 811, 935, 1297, 1429
FaqI	GGGAC	2 cut(s)	966, 1050
FatI	CATG	5 cut(s)	265, 807, 931, 1293, 1425
FbaI	TGATCA	2 cut(s)	769, 1054
Fnu4HI	GCNGC	4 cut(s)	629, 742, 928, 1406
FokI	GGATG	8 cut(s)	20, 368, 554, 800, 1208, 1268, 1279, 1373
FriOI	GRGCYC	2 cut(s)	640, 1115
Fsp4HI	GCNGC	4 cut(s)	629, 742, 928, 1406
FspBI	CTAG	2 cut(s)	42, 236
GluI	GCNGC	4 cut(s)	629, 742, 928, 1406
GsaI	CCCAGC	1 cut(s)	578
HaeIII	GGCC	1 cut(s)	289
HapII	CCGG	2 cut(s)	617, 1193
Hin1II	CATG	5 cut(s)	269, 811, 935, 1297, 1429
HincII	GTYRAC	1 cut(s)	1330
HindII	GTYRAC	1 cut(s)	1330
HindIII	AAGCTT	1 cut(s)	753
HinfI	GANTC	6 cut(s)	128, 170, 658, 954, 968, 1097
HpaI	GTTAAC	1 cut(s)	1330
HpaII	CCGG	2 cut(s)	617, 1193
HphI	GGTGA	1 cut(s)	1445
Hpy166II	GTNNAC	4 cut(s)	34, 519, 987, 1330
Hpy188I	TCNGA	5 cut(s)	101, 175, 525, 673, 774
Hpy188III	TCNNGA	8 cut(s)	65, 641, 842, 863, 1064, 1148, 1181, 1187
Hpy8I	GTNNAC	4 cut(s)	34, 519, 987, 1330
Hpy99I	CGWCG	1 cut(s)	564
HpyAV	CCTTC	1 cut(s)	940
HpyCH4III	ACNGT	3 cut(s)	23, 693, 1476
HpyCH4IV	ACGT	1 cut(s)	1039
HpyF10VI	GCNNNNNNNGC	8 cut(s)	55, 295, 323, 821, 845, 1090, 1163, 1405
HpyF3I	CTNAG	3 cut(s)	158, 384, 416
HpySE526I	ACGT	1 cut(s)	1039
Hsp92II	CATG	5 cut(s)	269, 811, 935, 1297, 1429
KroI	GCCGGC	1 cut(s)	616
KroNI	GCCGGC	1 cut(s)	618
Ksp22I	TGATCA	2 cut(s)	769, 1054
KspAI	GTTAAC	1 cut(s)	1330
Kzo9I	GATC	8 cut(s)	96, 525, 594, 748, 769, 1027, 1054, 1183
LmnI	GCTCC	3 cut(s)	635, 1118, 1171
Lsp1109I	GCAGC	4 cut(s)	640, 728, 939, 1392
LweI	GCATC	7 cut(s)	346, 532, 561, 753, 858, 1071, 1189
MaeI	CTAG	2 cut(s)	42, 236
MaeII	ACGT	1 cut(s)	1039
MaeIII	GTNAC	2 cut(s)	533, 1215
MalI	GATC	8 cut(s)	98, 527, 596, 750, 771, 1029, 1056, 1185
MboI	GATC	8 cut(s)	96, 525, 594, 748, 769, 1027, 1054, 1183
MboII	GAAGA	5 cut(s)	257, 404, 892, 1022, 1309
MflI	RGATCY	1 cut(s)	1183
MhlI	GDGCHC	3 cut(s)	626, 640, 1115
MluCI	AATT	6 cut(s)	183, 197, 281, 346, 1002, 1346
MlyI	GAGTC	3 cut(s)	948, 962, 1091
Mph1103I	ATGCAT	1 cut(s)	319
MroNI	GCCGGC	1 cut(s)	616
MseI	TTAA	2 cut(s)	1121, 1329
MslI	CAYNNNNRTG	2 cut(s)	117, 1166
MspA1I	CMGCKG	1 cut(s)	1405
MspI	CCGG	2 cut(s)	617, 1193
MspR9I	CCNGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
Mva1269I	GAATGC	2 cut(s)	319, 570
MvaI	CCWGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
MwoI	GCNNNNNNNGC	8 cut(s)	55, 295, 323, 821, 845, 1090, 1163, 1405
NaeI	GCCGGC	1 cut(s)	618
NdeII	GATC	8 cut(s)	96, 525, 594, 748, 769, 1027, 1054, 1183
NgoMIV	GCCGGC	1 cut(s)	616
NheI	GCTAGC	1 cut(s)	41
NlaIII	CATG	5 cut(s)	269, 811, 935, 1297, 1429
NlaIV	GGNNCC	1 cut(s)	1114
NsiI	ATGCAT	1 cut(s)	319
NspI	RCATGY	1 cut(s)	1429
PaeI	GCATGC	1 cut(s)	1429
PctI	GAATGC	2 cut(s)	319, 570
PdiI	GCCGGC	1 cut(s)	618
PfeI	GAWTC	3 cut(s)	128, 170, 658
PfoI	TCCNGGA	1 cut(s)	1262
PkrI	GCNGC	4 cut(s)	630, 743, 929, 1407
PleI	GAGTC	3 cut(s)	948, 962, 1091
PpsI	GAGTC	3 cut(s)	948, 962, 1091
PshAI	GACNNNNGTC	1 cut(s)	1464
Psp124BI	GAGCTC	1 cut(s)	640
Psp6I	CCWGG	6 cut(s)	289, 997, 1239, 1262, 1306, 1429
PspFI	CCCAGC	1 cut(s)	574
PspGI	CCWGG	6 cut(s)	289, 997, 1239, 1262, 1306, 1429
PspN4I	GGNNCC	1 cut(s)	1114
PspPI	GGNCC	1 cut(s)	287
PstI	CTGCAG	2 cut(s)	929, 1466
PstNI	CAGNNNCTG	3 cut(s)	173, 574, 1408
PsuI	RGATCY	1 cut(s)	1183
PvuII	CAGCTG	1 cut(s)	1405
RsaI	GTAC	4 cut(s)	518, 668, 805, 988
RsaNI	GTAC	4 cut(s)	517, 667, 804, 987
RseI	CAYNNNNRTG	2 cut(s)	117, 1166
SacI	GAGCTC	1 cut(s)	640
SaqAI	TTAA	2 cut(s)	1121, 1329
SatI	GCNGC	4 cut(s)	629, 742, 928, 1406
Sau3AI	GATC	8 cut(s)	96, 525, 594, 748, 769, 1027, 1054, 1183
Sau96I	GGNCC	1 cut(s)	287
SchI	GAGTC	3 cut(s)	948, 962, 1091
ScrFI	CCNGG	6 cut(s)	291, 999, 1241, 1264, 1308, 1431
SduI	GDGCHC	3 cut(s)	626, 640, 1115
SfaNI	GCATC	7 cut(s)	346, 532, 561, 753, 858, 1071, 1189
SfcI	CTRYAG	5 cut(s)	19, 689, 925, 1400, 1462
SmiMI	CAYNNNNRTG	2 cut(s)	117, 1166
SmlI	CTYRAG	4 cut(s)	65, 641, 956, 1148
SmoI	CTYRAG	4 cut(s)	65, 641, 956, 1148
SphI	GCATGC	1 cut(s)	1429
Sse9I	AATT	6 cut(s)	183, 197, 281, 346, 1002, 1346
SspMI	CTAG	2 cut(s)	42, 236
SstI	GAGCTC	1 cut(s)	640
StyD4I	CCNGG	6 cut(s)	289, 997, 1239, 1262, 1306, 1429
TaaI	ACNGT	3 cut(s)	23, 693, 1476
TaiI	ACGT	1 cut(s)	1042
TaqI	TCGA	4 cut(s)	559, 582, 747, 1065
TaqII	GACCGA	1 cut(s)	685
TasI	AATT	6 cut(s)	183, 197, 281, 346, 1002, 1346
TfiI	GAWTC	3 cut(s)	128, 170, 658
Tru1I	TTAA	2 cut(s)	1121, 1329
Tru9I	TTAA	2 cut(s)	1121, 1329
TscAI	CASTG	3 cut(s)	28, 465, 604
TseI	GCWGC	4 cut(s)	628, 741, 927, 1405
TspDTI	ATGAA	4 cut(s)	254, 390, 1103, 1310
TspRI	CASTG	3 cut(s)	28, 465, 604
XagI	CCTNNNNNAGG	1 cut(s)	1055
XapI	RAATTY	3 cut(s)	197, 281, 1346
XceI	RCATGY	1 cut(s)	1429
XspI	CTAG	2 cut(s)	42, 236
Zsp2I	ATGCAT	1 cut(s)	319

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.