pycom15g09180

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014514
Physical Location & Seq
Forward (+)
5918953 .. 5920695
1743 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g09180.2

Sequence Viewer

Length: 1581 bp
ATGAGCCTGCAGTCCCTGATCAAGCAGCAAATTCTTCGAACAGTTCCGAAGGATATTCGCCAACCTCTGCCGCTCATTCATCCTTCCGAGCAACCCCGTTTTGCAAAGTCCCTAATGGCAACGAGATTCAGCGCCTTATCTGTCAGAGAACTGGAAAATTCCTTCGTACCCAGGTTACTAAATTGCAAAAGCACAAAAGGGTTGAAGAAAATTCACGCTCAGATTGTGAAGCTCTCGTTGTCACAGAGCAATTTCTTGGTGACAAAAATGGTGGACGTGTGTGACAACAGTGGGGATTTGCGCTACGCTGGTTTGCTATTCGAACAAGTTATGGAATCAAACGTGTTTCTGTTCAACGCCACGATCAGAGCTTATACGCATAATCAAATGTATGATTTAGCAATCAATTTGTATAAGCAAATGCTGAGAGAAGCAGAGAACCAAAAACTTCCTGACAAATTCACGTTCCCATTTGTGATCAAATCTTGCGCGGCGATATTCTGTGAAGTTTTGGGGAGGCAAGTTCATGCCCAAGTGTGTAAATTTGGGCCGAAGTCGCATTTGCTGATTGAGAATGCTTTGATGGATTTGTATTTGAAATGTGATAATTTAGCAGATGCACACAAGGTGTTTGACGGAATGTCTGAACCGGATGTCATTTCTTGGAATAGTCTCCTTTCTGGGTATGTTAGATTGGGGAAGATGAAAGGGGCAAGGGCAGTGTTTGAGGAAATTCCTAACAAGACTATTGTGTCTTGGACAGCAATGGTTTCAGGGTATACGAGAATCAGGTGCTACGCCGAAGCTCTGGATATGTTCCGGCGAATGCAAATGCTGGATATTGAACCTGATGAGATTAGTATTGTTTCTGTTTTGCCGGCTTGCGCACAGCTTGGAGCTCTTGAGGTTGGGAAATGGATTCACATGTACTCGGAAAAGAAGCTGCTATTGCAGTCGGTTTGTGTTTGCAATGCCCTGATTGAAATGTACGTGAAGTGTGGCTGCATTGAGCAGGCTTGGCACTTGTTTGATCAGATGCTGGAGAGGGATGTGATTTCTTGGAGTACCATGATAGCAGGGCTGGCGAATTACGGGAAAGCTCGCGAAGCAGTTGAACTGTTTCAAGAGATGCAAATAGTGAAGGTTGAACCGAATGGTGCTCTTGACAGAATAAACAAGATGCCGATGAATCCAGGCTCAAAGATTTGGGGTTCGTTGTTAAGTTCCTGCAGGACTCATTGCAATCTCGAGATTGCCATTACCGCAATGGAGCACCTTTCGGTGCTTGAACCAGATGAGGCAGGTAACTATGTGCTACTTTCCAATATATATGCAGACCTAGGGAAGTGGGAGGATGTGTCAAGGATGAGGAAACTTATAAGAAGTAAGAGCATGAAGAGAACCCCAGGTTGCAGTTCGATCGAGGTTGACAATGTGGCTATGGAATTTGTGGCCGGTGACGATTCAAAACCATTTGCGAGAGACATCTTTTCCATGCTAGAGCTGCTTCTTTCGGAGCAGAACATACCAGATGATAAGTTCGACGCAACACCAGAGGACTGCAGTCAATTCTTGTGTTAA

Protein Analysis

527

Amino Acids

59.57

Weight (kDa)

6.4

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014514)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G20540 AT2G20540
fragaria_vesca FvH4_2g36320
malus_domestica MD15G1099800.v1.1
prunus_persica Prupe.1G454600_v2.0.a1
pyrus_communis pycom15g09180
rosa_chinensis RchiOBHm_Chr6g0305561
rosa_laevigata RLG00000010877
rosa_multiflora Rmu_co8430547.1_g000001 Rmu_sc0000841.1_g000002 Rmu_sc0001153.1_g000005
rosa_roxburghii Rroxscaffold_7G00162590
rosa_rugosa Rorug06G0341700
rosa_samantha Rh6AG453700 Rh6BG447900 Rh6DG454000
rosa_wichuraiana Rw6G039540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1379
AasI GACNNNNNNGTC 1 cut(s) 751
Acc16I TGCGCA 1 cut(s) 886
Acc36I ACCTGC 1 cut(s) 1292
AccBSI CCGCTC 1 cut(s) 73
AccI GTMKAC 1 cut(s) 779
AccII CGCG 2 cut(s) 491, 1104
AciI CCGC 3 cut(s) 71, 491, 1263
AcoI YGGCCR 1 cut(s) 1452
AcsI RAATTY 7 cut(s) 30, 157, 210, 458, 542, 732, 1445
AdeI CACNNNGTG 1 cut(s) 628
AfaI GTAC 4 cut(s) 168, 929, 989, 1066
AflIII ACRYGT 3 cut(s) 276, 342, 924
AhdI GACNNNNNGTC 1 cut(s) 640
AjiI CACGTC 1 cut(s) 277
AjnI CCWGG 3 cut(s) 170, 1192, 1405
AluBI AGCT 8 cut(s) 232, 371, 806, 892, 899, 943, 1100, 1504
AluI AGCT 8 cut(s) 232, 371, 806, 892, 899, 943, 1100, 1504
Alw21I GWGCWC 3 cut(s) 901, 1162, 1275
Alw26I GTCTC 2 cut(s) 677, 1476
AlwNI CAGNNNCTG 2 cut(s) 16, 1039
Ama87I CYCGRG 1 cut(s) 1247
AoxI GGCC 2 cut(s) 548, 1452
ApeKI GCWGC 4 cut(s) 25, 943, 1002, 1504
ApoI RAATTY 7 cut(s) 30, 157, 210, 458, 542, 732, 1445
Asp700I GAANNNNTTC 1 cut(s) 1119
AspA2I CCTAGG 1 cut(s) 1339
AspLEI GCGC 4 cut(s) 134, 303, 491, 887
AspS9I GGNCC 1 cut(s) 548
AsuHPI GGTGA 2 cut(s) 271, 1469
AsuII TTCGAA 2 cut(s) 37, 321
AvaI CYCGRG 1 cut(s) 1247
AvrII CCTAGG 1 cut(s) 1339
BanII GRGCYC 1 cut(s) 901
Bbv12I GWGCWC 3 cut(s) 901, 1162, 1275
BbvI GCAGC 4 cut(s) 37, 930, 989, 1491
BccI CCATC 1 cut(s) 577
BcgI CGANNNNNNTGC 4 cut(s) 17, 51, 1402, 1436
BciT130I CCWGG 3 cut(s) 172, 1194, 1407
BclI TGATCA 3 cut(s) 18, 477, 1030
BcoDI GTCTC 2 cut(s) 677, 1476
BfaI CTAG 2 cut(s) 1340, 1499
BfmI CTRYAG 3 cut(s) 8, 1228, 1561
BfoI RGCGCY 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 1292
BisI GCNGC 6 cut(s) 26, 71, 492, 944, 1003, 1505
BlnI CCTAGG 1 cut(s) 1339
BlsI GCNGC 6 cut(s) 27, 72, 493, 945, 1004, 1506
Bme1390I CCNGG 3 cut(s) 172, 1194, 1407
BmeRI GACNNNNNGTC 1 cut(s) 640
BmeT110I CYCGRG 1 cut(s) 1247
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 548
BmrFI CCNGG 3 cut(s) 172, 1194, 1407
BmsI GCATC 4 cut(s) 607, 1026, 1119, 1170
BoxI GACNNNNGTC 1 cut(s) 1563
BpmI CTGGAG 1 cut(s) 1061
Bpu14I TTCGAA 2 cut(s) 37, 321
BpuEI CTTGAG 1 cut(s) 923
BsaAI YACGTR 1 cut(s) 991
BsaJI CCNNGG 3 cut(s) 170, 1339, 1405
BsaWI WCCGGW 1 cut(s) 649
BsaXI ACNNNNNCTCC 2 cut(s) 1343, 1373
Bse118I RCCGGY 2 cut(s) 877, 1454
Bse1I ACTGG 1 cut(s) 156
Bse3DI GCAATG 4 cut(s) 771, 976, 1237, 1272
BseBI CCWGG 3 cut(s) 172, 1194, 1407
BseDI CCNNGG 3 cut(s) 170, 1339, 1405
BseGI GGATG 5 cut(s) 79, 658, 1054, 1360, 1371
BseMI GCAATG 4 cut(s) 771, 976, 1237, 1272
BseMII CTCAG 2 cut(s) 233, 416
BseNI ACTGG 1 cut(s) 156
BseXI GCAGC 4 cut(s) 37, 930, 989, 1491
Bsh1236I CGCG 2 cut(s) 491, 1104
Bsh1285I CGRYCG 1 cut(s) 1422
BshFI GGCC 2 cut(s) 550, 1454
BsiEI CGRYCG 1 cut(s) 1422
BsiHKAI GWGCWC 3 cut(s) 901, 1162, 1275
BsiHKCI CYCGRG 1 cut(s) 1247
BsiSI CCGG 4 cut(s) 650, 820, 878, 1455
BslFI GGGAC 1 cut(s) 94
BsmAI GTCTC 2 cut(s) 677, 1476
BsmFI GGGAC 1 cut(s) 94
BsmI GAATGC 2 cut(s) 580, 831
BsnI GGCC 2 cut(s) 550, 1454
BsoBI CYCGRG 1 cut(s) 1247
Bsp119I TTCGAA 2 cut(s) 37, 321
Bsp1286I GDGCHC 3 cut(s) 901, 1162, 1275
Bsp143I GATC 5 cut(s) 18, 363, 477, 1030, 1419
Bsp68I TCGCGA 1 cut(s) 1104
BspACI CCGC 3 cut(s) 71, 491, 1263
BspANI GGCC 2 cut(s) 550, 1454
BspCNI CTCAG 2 cut(s) 232, 417
BspFNI CGCG 2 cut(s) 491, 1104
BspMAI CTGCAG 3 cut(s) 12, 1232, 1565
BspMI ACCTGC 1 cut(s) 1292
BspT104I TTCGAA 2 cut(s) 37, 321
BsrBI CCGCTC 1 cut(s) 73
BsrDI GCAATG 4 cut(s) 771, 976, 1237, 1272
BsrFI RCCGGY 2 cut(s) 877, 1454
BsrI ACTGG 1 cut(s) 156
BssAI RCCGGY 2 cut(s) 877, 1454
BssECI CCNNGG 3 cut(s) 170, 1339, 1405
BssMI GATC 5 cut(s) 18, 363, 477, 1030, 1419
BssNAI GTATAC 1 cut(s) 780
BssT1I CCWWGG 1 cut(s) 1339
Bst1107I GTATAC 1 cut(s) 780
Bst2UI CCWGG 3 cut(s) 172, 1194, 1407
Bst4CI ACNGT 3 cut(s) 43, 290, 1119
Bst6I CTCTTC 1 cut(s) 1391
BstBAI YACGTR 1 cut(s) 991
BstBI TTCGAA 2 cut(s) 37, 321
BstC8I GCNNGC 6 cut(s) 8, 879, 883, 1014, 1083, 1102
BstDEI CTNAG 2 cut(s) 219, 425
BstF5I GGATG 5 cut(s) 79, 658, 1054, 1360, 1371
BstFNI CGCG 2 cut(s) 491, 1104
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 4 cut(s) 134, 303, 491, 887
BstKTI GATC 5 cut(s) 21, 366, 480, 1033, 1422
BstMAI GTCTC 2 cut(s) 677, 1476
BstMBI GATC 5 cut(s) 18, 363, 477, 1030, 1419
BstMCI CGRYCG 1 cut(s) 1422
BstMWI GCNNNNNNNGC 7 cut(s) 556, 949, 1018, 1082, 1106, 1262, 1504
BstNI CCWGG 3 cut(s) 172, 1194, 1407
BstNSI RCATGY 1 cut(s) 928
BstPAI GACNNNNGTC 1 cut(s) 1563
BstSCI CCNGG 3 cut(s) 170, 1192, 1405
BstSFI CTRYAG 3 cut(s) 8, 1228, 1561
BstUI CGCG 2 cut(s) 491, 1104
BstV1I GCAGC 4 cut(s) 37, 930, 989, 1491
BstZ17I GTATAC 1 cut(s) 780
BsuRI GGCC 2 cut(s) 550, 1454
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 5 cut(s) 79, 658, 1054, 1360, 1371
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 2 cut(s) 295, 726
BtuMI TCGCGA 1 cut(s) 1104
BveI ACCTGC 1 cut(s) 1292
Cac8I GCNNGC 6 cut(s) 8, 879, 883, 1014, 1083, 1102
CaiI CAGNNNCTG 2 cut(s) 16, 1039
CfoI GCGC 4 cut(s) 134, 303, 491, 887
Cfr10I RCCGGY 2 cut(s) 877, 1454
Cfr13I GGNCC 1 cut(s) 548
CseI GACGC 1 cut(s) 1553
Csp6I GTAC 4 cut(s) 167, 928, 988, 1065
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 5 cut(s) 527, 925, 1069, 1393, 1495
CviQI GTAC 4 cut(s) 167, 928, 988, 1065
DdeI CTNAG 2 cut(s) 219, 425
DpnI GATC 5 cut(s) 20, 365, 479, 1032, 1421
DpnII GATC 5 cut(s) 18, 363, 477, 1030, 1419
DraIII CACNNNGTG 1 cut(s) 628
DrdI GACNNNNNNGTC 1 cut(s) 751
DriI GACNNNNNGTC 1 cut(s) 640
DseDI GACNNNNNNGTC 1 cut(s) 751
EaeI YGGCCR 1 cut(s) 1452
Eam1104I CTCTTC 1 cut(s) 1391
Eam1105I GACNNNNNGTC 1 cut(s) 640
EarI CTCTTC 1 cut(s) 1391
Ecl136II GAGCTC 1 cut(s) 899
Eco130I CCWWGG 1 cut(s) 1339
Eco24I GRGCYC 1 cut(s) 901
Eco53kI GAGCTC 1 cut(s) 899
Eco88I CYCGRG 1 cut(s) 1247
EcoICRI GAGCTC 1 cut(s) 899
EcoRII CCWGG 3 cut(s) 170, 1192, 1405
EcoT14I CCWWGG 1 cut(s) 1339
EcoT38I GRGCYC 1 cut(s) 901
ErhI CCWWGG 1 cut(s) 1339
FaeI CATG 5 cut(s) 530, 928, 1072, 1396, 1498
FaqI GGGAC 1 cut(s) 94
FatI CATG 5 cut(s) 526, 924, 1068, 1392, 1494
FbaI TGATCA 3 cut(s) 18, 477, 1030
FblI GTMKAC 1 cut(s) 779
Fnu4HI GCNGC 6 cut(s) 26, 71, 492, 944, 1003, 1505
FokI GGATG 5 cut(s) 66, 665, 1061, 1367, 1378
FriOI GRGCYC 1 cut(s) 901
Fsp4HI GCNGC 6 cut(s) 26, 71, 492, 944, 1003, 1505
FspBI CTAG 2 cut(s) 1340, 1499
FspI TGCGCA 1 cut(s) 886
GlaI GCGC 4 cut(s) 133, 302, 490, 886
GluI GCNGC 6 cut(s) 26, 71, 492, 944, 1003, 1505
GsuI CTGGAG 1 cut(s) 1061
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 2 cut(s) 550, 1454
HapII CCGG 4 cut(s) 650, 820, 878, 1455
HgaI GACGC 1 cut(s) 1553
HhaI GCGC 4 cut(s) 134, 303, 491, 887
Hin1II CATG 5 cut(s) 530, 928, 1072, 1396, 1498
Hin6I GCGC 4 cut(s) 132, 301, 489, 885
HinP1I GCGC 4 cut(s) 132, 301, 489, 885
HincII GTYRAC 1 cut(s) 1429
HindII GTYRAC 1 cut(s) 1429
HinfI GANTC 7 cut(s) 126, 335, 786, 919, 1189, 1234, 1463
HpaII CCGG 4 cut(s) 650, 820, 878, 1455
HphI GGTGA 2 cut(s) 271, 1469
Hpy166II GTNNAC 3 cut(s) 274, 780, 1429
Hpy188I TCNGA 9 cut(s) 48, 88, 146, 222, 368, 646, 934, 1035, 1516
Hpy188III TCNNGA 8 cut(s) 452, 809, 902, 1103, 1124, 1163, 1247, 1249
Hpy8I GTNNAC 3 cut(s) 274, 780, 1429
Hpy99I CGWCG 1 cut(s) 1547
HpyAV CCTTC 4 cut(s) 43, 93, 172, 1135
HpyCH4III ACNGT 3 cut(s) 43, 290, 1119
HpyCH4IV ACGT 4 cut(s) 276, 342, 464, 990
HpyF10VI GCNNNNNNNGC 7 cut(s) 556, 949, 1018, 1082, 1106, 1262, 1504
HpyF3I CTNAG 2 cut(s) 219, 425
HpySE526I ACGT 4 cut(s) 276, 342, 464, 990
Hsp92II CATG 5 cut(s) 530, 928, 1072, 1396, 1498
HspAI GCGC 4 cut(s) 132, 301, 489, 885
KroI GCCGGC 1 cut(s) 877
KroNI GCCGGC 1 cut(s) 879
Ksp22I TGATCA 3 cut(s) 18, 477, 1030
Kzo9I GATC 5 cut(s) 18, 363, 477, 1030, 1419
LmnI GCTCC 3 cut(s) 896, 1270, 1516
Lsp1109I GCAGC 4 cut(s) 37, 930, 989, 1491
LweI GCATC 4 cut(s) 607, 1026, 1119, 1170
MaeI CTAG 2 cut(s) 1340, 1499
MaeII ACGT 4 cut(s) 276, 342, 464, 990
MaeIII GTNAC 6 cut(s) 174, 240, 259, 281, 1304, 1457
MalI GATC 5 cut(s) 20, 365, 479, 1032, 1421
MbiI CCGCTC 1 cut(s) 73
MboI GATC 5 cut(s) 18, 363, 477, 1030, 1419
MboII GAAGA 4 cut(s) 26, 217, 712, 1408
MhlI GDGCHC 3 cut(s) 901, 1162, 1275
MlyI GAGTC 1 cut(s) 1228
MroNI GCCGGC 1 cut(s) 877
MroXI GAANNNNTTC 1 cut(s) 1119
MseI TTAA 2 cut(s) 1220, 1579
MspI CCGG 4 cut(s) 650, 820, 878, 1455
MspR9I CCNGG 3 cut(s) 172, 1194, 1407
Mva1269I GAATGC 2 cut(s) 580, 831
MvaI CCWGG 3 cut(s) 172, 1194, 1407
MvnI CGCG 2 cut(s) 491, 1104
MwoI GCNNNNNNNGC 7 cut(s) 556, 949, 1018, 1082, 1106, 1262, 1504
NaeI GCCGGC 1 cut(s) 879
NdeII GATC 5 cut(s) 18, 363, 477, 1030, 1419
NgoMIV GCCGGC 1 cut(s) 877
NlaIII CATG 5 cut(s) 530, 928, 1072, 1396, 1498
NmuCI GTSAC 4 cut(s) 240, 259, 281, 1457
NruI TCGCGA 1 cut(s) 1104
NsbI TGCGCA 1 cut(s) 886
NspI RCATGY 1 cut(s) 928
NspV TTCGAA 2 cut(s) 37, 321
PaeR7I CTCGAG 1 cut(s) 1247
PciI ACATGT 1 cut(s) 924
PctI GAATGC 2 cut(s) 580, 831
PdiI GCCGGC 1 cut(s) 879
PdmI GAANNNNTTC 1 cut(s) 1119
PfeI GAWTC 6 cut(s) 126, 335, 786, 919, 1189, 1463
PkrI GCNGC 6 cut(s) 27, 72, 493, 945, 1004, 1506
Ple19I CGATCG 1 cut(s) 1422
PleI GAGTC 1 cut(s) 1228
PpsI GAGTC 1 cut(s) 1228
Ppu21I YACGTR 1 cut(s) 991
PscI ACATGT 1 cut(s) 924
PshAI GACNNNNGTC 1 cut(s) 1563
PsiI TTATAA 1 cut(s) 1379
Psp124BI GAGCTC 1 cut(s) 901
Psp6I CCWGG 3 cut(s) 170, 1192, 1405
PspGI CCWGG 3 cut(s) 170, 1192, 1405
PspPI GGNCC 1 cut(s) 548
PstI CTGCAG 3 cut(s) 12, 1232, 1565
PstNI CAGNNNCTG 2 cut(s) 16, 1039
PvuI CGATCG 1 cut(s) 1422
RruI TCGCGA 1 cut(s) 1104
RsaI GTAC 4 cut(s) 168, 929, 989, 1066
RsaNI GTAC 4 cut(s) 167, 928, 988, 1065
SacI GAGCTC 1 cut(s) 901
SaqAI TTAA 2 cut(s) 1220, 1579
SatI GCNGC 6 cut(s) 26, 71, 492, 944, 1003, 1505
Sau3AI GATC 5 cut(s) 18, 363, 477, 1030, 1419
Sau96I GGNCC 1 cut(s) 548
SbfI CCTGCAGG 1 cut(s) 1232
SchI GAGTC 1 cut(s) 1228
ScrFI CCNGG 3 cut(s) 172, 1194, 1407
SdaI CCTGCAGG 1 cut(s) 1232
SduI GDGCHC 3 cut(s) 901, 1162, 1275
SfaNI GCATC 4 cut(s) 607, 1026, 1119, 1170
SfcI CTRYAG 3 cut(s) 8, 1228, 1561
Sfr274I CTCGAG 1 cut(s) 1247
SfuI TTCGAA 2 cut(s) 37, 321
SlaI CTCGAG 1 cut(s) 1247
SmlI CTYRAG 2 cut(s) 902, 1247
SmoI CTYRAG 2 cut(s) 902, 1247
Sse8387I CCTGCAGG 1 cut(s) 1232
SsiI CCGC 3 cut(s) 71, 491, 1263
SspMI CTAG 2 cut(s) 1340, 1499
SstI GAGCTC 1 cut(s) 901
StyD4I CCNGG 3 cut(s) 170, 1192, 1405
StyI CCWWGG 1 cut(s) 1339
TaaI ACNGT 3 cut(s) 43, 290, 1119
TaiI ACGT 4 cut(s) 279, 345, 467, 993
TaqI TCGA 6 cut(s) 37, 321, 1248, 1418, 1422, 1542
TatI WGTACW 1 cut(s) 927
TauI GCSGC 2 cut(s) 73, 494
TfiI GAWTC 6 cut(s) 126, 335, 786, 919, 1189, 1463
Tru1I TTAA 2 cut(s) 1220, 1579
Tru9I TTAA 2 cut(s) 1220, 1579
TscAI CASTG 2 cut(s) 295, 726
TseFI GTSAC 4 cut(s) 240, 259, 281, 1457
TseI GCWGC 4 cut(s) 25, 943, 1002, 1504
Tsp45I GTSAC 4 cut(s) 240, 259, 281, 1457
TspDTI ATGAA 5 cut(s) 68, 515, 719, 1202, 1409
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 2 cut(s) 295, 726
XapI RAATTY 7 cut(s) 30, 157, 210, 458, 542, 732, 1445
XceI RCATGY 1 cut(s) 928
XcmI CCANNNNNNNNNTGG 1 cut(s) 1264
XhoI CTCGAG 1 cut(s) 1247
XmaJI CCTAGG 1 cut(s) 1339
XmiI GTMKAC 1 cut(s) 779
XmnI GAANNNNTTC 1 cut(s) 1119
XspI CTAG 2 cut(s) 1340, 1499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.