FvH4_2g36990

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015128
Physical Location & Seq
Forward (+)
26997088 .. 27000634
3547 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36990.t1

Sequence Viewer

Length: 1623 bp
ATGTCAGTTGATATGGCTAGTGAGAGAGATCTCCTATCCACCGAGATCGTCAACAATGGAATCGAATCCTCCGGCCCCAATGCTGGCTCCCTCACTTTCTCCGTCAGGGTCCGGAGGCGCCTGCCGGACTTTCTCCAGTCGGTGAACCTCAAGTACGTCAAGTTGGGGTACCACCACCTCATCAATAATGCTATATACCTGGCCACCATACCTATTTTAGTGCTGGTGTTCAGTGCTGAGGTTGGGAGTCTCAGCAGGGAGGAGCTGTGGAAGAAGCTCTGGTTTGATGCTCGTTATGACCTAGCCACTGTGCTTGGCTTCTTCGGGGTCTTTGTCTTCACTTTGTCTGTCTACTTTATGTCTCGCCCTCGCTCCATCTATCTCATTGATTTTGCTTGCTTTCGCCCTAGTGATGATCTAAAGGTGTCAAAGGAGCAATTCATAGAGCTAGCAAGAAAATCAGGCAAGTTTGATGAACAGAGCCTGGAATTCCAGGCTCGAATTCTCAACTCATCAGGTTTAGGCAACGAGACCTACATCCCAAAGTCAGTGATCACTTCCACTGAGAACACTGCAACCATGAAAGAAGGTCGAGCCGAGGCCTCACTGGTGATGTTCGGAGCACTTGACGAGCTCTTTGAAAAGACCAAGGTCCGTCCCAAGGACGTAGGTGTTCTTGTGGTGAACTGCAGCATATTCAATCCCACACCCTCCTTGTCCGCCATGATCATAAACCACTACAAAATGAGGGGCAACATTTTGAGCTACAACCTGGGTGGCATGGGCTGCAGCGCTGGGATCATAGCCGTTGATTTGGCTAGGGACATGCTTCAGGCAAATCCCAACAACTATGCTGTGGTGGTGAGCACCGAGACGGTGGGATATAATTGGTACCCGGGAAGTGATAGGTCTATGCTCGTTCCCAACTGTTTCTTCCGCATGGGTTGCTCCGCCGTGCTTCTCTCCAATCGCCGCCGTGACTACCACCGTGCCAAGTATCGCCTCGAACACATCGTTCGAACACATAAAGGCGCCGACGATCGTAGCTTCAGGTGTGTGTACCAAGAAGAAGATGAGCAACGGCTCAAGGGGCTAAAGATCAGCAAAGAGCTAGTGGAAATCGGTGGTGAGGCACTGAAAACCAACATCACCACTCTCGGACCTCTAGTCCTTCCCTTCTCGGAGCAGCTCCTCTTCTTCGCTACATTGGTCTCGAGGCACTTGTTCAACAAAGAAGGTTCACAGCTCTCTTTATCTTCGGGCAAGCAGAGTCGACCCTACATTCCTGATTTCAAGCTGGCATTCGAGCGCTTCTGTGTGCACGCCGCGAGCAAGACTGTGCTGGATGAGCTGCAGAGAAACCTCGAGCTCAGCGAAGAGAACATGGAGGCGTCGAGGATGACGCTGCACCGATTTGGTAACACTTCGAGCAGTAGCATATGGTATGAGCTGGCTTACATGGAAGCAAAGGAGAAAGTGAAGAGAGGAGACCGAGTTTGGCAGTTGGCGTTCGGGTCTGGATTCAAGTGCAACAGCATTGTTTGGAGGTCCATGCGCCGGGTTAGGAAGCCTTCAAGGAATCCATGGCTGGATTGCATTGAAAGATACCCAGTCATGAATTGA

Protein Analysis

541

Amino Acids

61.39

Weight (kDa)

9.14

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 118 - 407 1.2e-132 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 436 - 516 2.5e-13 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 438 - 518 1.1e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015128)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26250
fragaria_vesca FvH4_2g36990
malus_domestica MD08G1046600.v1.1
prunus_persica Prupe.1G401500_v2.0.a1
pyrus_communis pycom08g03880 pycom15g04560
rosa_chinensis RchiOBHm_Chr6g0304771
rosa_laevigata RLG00000010951
rosa_multiflora Rmu_sc0003120.1_g000005
rosa_roxburghii Rroxscaffold_7G00163510
rosa_rugosa Rorug06G0334400
rosa_samantha Rh6AG446300 Rh6BG455300 Rh6CG459800 Rh6DG446600
rosa_wichuraiana Rw6G038830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 168, 891
AccB1I GGYRCC 4 cut(s) 117, 168, 891, 1031
AccI GTMKAC 2 cut(s) 351, 1273
AccII CGCG 1 cut(s) 1328
AccIII TCCGGA 1 cut(s) 111
AciI CCGC 5 cut(s) 720, 937, 951, 973, 1326
AclWI GGATC 1 cut(s) 806
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 2 cut(s) 488, 501
AcuI CTGAAG 2 cut(s) 815, 1033
AcyI GRCGYC 3 cut(s) 118, 1032, 1391
AfaI GTAC 4 cut(s) 155, 170, 893, 1061
AfeI AGCGCT 2 cut(s) 793, 1310
AfiI CCNNNNNNNGG 3 cut(s) 83, 661, 1557
AgsI TTSAA 7 cut(s) 641, 700, 1228, 1294, 1525, 1575, 1601
AhdI GACNNNNNGTC 1 cut(s) 1166
AjnI CCWGG 4 cut(s) 198, 483, 492, 771
AjuI GAANNNNNNNTTGG 2 cut(s) 917, 949
Alw21I GWGCWC 5 cut(s) 625, 636, 869, 1323, 1371
Alw26I GTCTC 6 cut(s) 254, 366, 524, 866, 1216, 1482
Alw44I GTGCAC 1 cut(s) 1319
AlwI GGATC 1 cut(s) 806
AlwNI CAGNNNCTG 1 cut(s) 484
Ama87I CYCGRG 3 cut(s) 895, 1213, 1364
Aor13HI TCCGGA 1 cut(s) 111
Aor51HI AGCGCT 2 cut(s) 793, 1310
AoxI GGCC 3 cut(s) 73, 201, 600
ApaLI GTGCAC 1 cut(s) 1319
ApeKI GCWGC 6 cut(s) 690, 786, 789, 1186, 1351, 1405
ApoI RAATTY 2 cut(s) 488, 501
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
Asp718I GGTACC 2 cut(s) 168, 891
AspLEI GCGC 5 cut(s) 120, 794, 1034, 1311, 1557
AspS9I GGNCC 5 cut(s) 74, 109, 652, 1160, 1548
AsuC2I CCSGG 3 cut(s) 896, 897, 1559
AsuHPI GGTGA 6 cut(s) 154, 622, 694, 874, 1139, 1141
AsuII TTCGAA 1 cut(s) 1018
AsuNHI GCTAGC 1 cut(s) 448
AvaI CYCGRG 3 cut(s) 895, 1213, 1364
AvaII GGWCC 4 cut(s) 109, 652, 1160, 1548
BaeGI GKGCMC 1 cut(s) 1323
BalI TGGCCA 1 cut(s) 203
BanI GGYRCC 4 cut(s) 117, 168, 891, 1031
BanII GRGCYC 2 cut(s) 636, 1371
BbsI GAAGAC 1 cut(s) 328
Bbv12I GWGCWC 5 cut(s) 625, 636, 869, 1323, 1371
BbvCI CCTCAGC 1 cut(s) 237
BbvI GCAGC 6 cut(s) 702, 773, 801, 1198, 1338, 1392
BccI CCATC 1 cut(s) 383
BceAI ACGGC 4 cut(s) 791, 938, 960, 1097
BciT130I CCWGG 4 cut(s) 200, 485, 494, 773
BclI TGATCA 2 cut(s) 552, 726
BcnI CCSGG 3 cut(s) 896, 897, 1559
BcoDI GTCTC 6 cut(s) 254, 366, 524, 866, 1216, 1482
BfaI CTAG 7 cut(s) 18, 302, 408, 449, 819, 1112, 1166
BfmI CTRYAG 3 cut(s) 688, 787, 1352
BfoI RGCGCY 4 cut(s) 121, 795, 1035, 1312
BglII AGATCT 1 cut(s) 28
BisI GCNGC 8 cut(s) 691, 787, 790, 973, 1187, 1326, 1352, 1406
BlpI GCTNAGC 1 cut(s) 1370
BlsI GCNGC 8 cut(s) 692, 788, 791, 974, 1188, 1327, 1353, 1407
Bme1390I CCNGG 7 cut(s) 200, 485, 494, 773, 896, 897, 1559
Bme18I GGWCC 4 cut(s) 109, 652, 1160, 1548
BmeRI GACNNNNNGTC 1 cut(s) 1166
BmeT110I CYCGRG 3 cut(s) 895, 1213, 1364
BmgT120I GGNCC 5 cut(s) 74, 109, 652, 1160, 1548
BmiI GGNNCC 7 cut(s) 76, 88, 110, 119, 170, 893, 1033
BmrFI CCNGG 7 cut(s) 200, 485, 494, 773, 896, 897, 1559
BmrI ACTGGG 1 cut(s) 1604
BmsI GCATC 1 cut(s) 277
BmtI GCTAGC 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 1604
BoxI GACNNNNGTC 1 cut(s) 650
BpiI GAAGAC 1 cut(s) 328
BpmI CTGGAG 1 cut(s) 119
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 1370
Bpu14I TTCGAA 1 cut(s) 1018
BpuEI CTTGAG 2 cut(s) 134, 1070
BpuMI CCSGG 3 cut(s) 896, 897, 1559
BsaHI GRCGYC 3 cut(s) 118, 1032, 1391
BsaI GGTCTC 3 cut(s) 524, 1216, 1482
BsaJI CCNNGG 6 cut(s) 597, 648, 660, 772, 895, 1583
BsaWI WCCGGW 1 cut(s) 111
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 1479, 1509
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 661, 1557
Bse1I ACTGG 3 cut(s) 136, 612, 1610
BseAI TCCGGA 1 cut(s) 111
BseBI CCWGG 4 cut(s) 200, 485, 494, 773
BseDI CCNNGG 6 cut(s) 597, 648, 660, 772, 895, 1583
BseGI GGATG 3 cut(s) 537, 1351, 1404
BseLI CCNNNNNNNGG 3 cut(s) 83, 661, 1557
BseMII CTCAG 4 cut(s) 228, 265, 555, 1384
BseNI ACTGG 3 cut(s) 136, 612, 1610
BseRI GAGGAG 3 cut(s) 275, 1181, 1500
BseSI GKGCMC 1 cut(s) 1323
BseXI GCAGC 6 cut(s) 702, 773, 801, 1198, 1338, 1392
BseYI CCCAGC 1 cut(s) 794
BsgI GTGCAG 1 cut(s) 1391
Bsh1236I CGCG 1 cut(s) 1328
Bsh1285I CGRYCG 1 cut(s) 1042
BshFI GGCC 3 cut(s) 75, 203, 602
BshNI GGYRCC 4 cut(s) 117, 168, 891, 1031
BsiEI CGRYCG 1 cut(s) 1042
BsiHKAI GWGCWC 5 cut(s) 625, 636, 869, 1323, 1371
BsiHKCI CYCGRG 3 cut(s) 895, 1213, 1364
BsiSI CCGG 5 cut(s) 72, 112, 125, 896, 1558
BslFI GGGAC 2 cut(s) 642, 836
BslI CCNNNNNNNGG 3 cut(s) 83, 661, 1557
BsmAI GTCTC 6 cut(s) 254, 366, 524, 866, 1216, 1482
BsmBI CGTCTC 1 cut(s) 866
BsmFI GGGAC 2 cut(s) 642, 836
BsmI GAATGC 1 cut(s) 1301
BsnI GGCC 3 cut(s) 75, 203, 602
Bso31I GGTCTC 3 cut(s) 524, 1216, 1482
BsoBI CYCGRG 3 cut(s) 895, 1213, 1364
Bsp119I TTCGAA 1 cut(s) 1018
Bsp1286I GDGCHC 5 cut(s) 625, 636, 869, 1323, 1371
Bsp13I TCCGGA 1 cut(s) 111
Bsp143I GATC 8 cut(s) 28, 45, 415, 552, 726, 798, 1039, 1098
Bsp1720I GCTNAGC 1 cut(s) 1370
Bsp19I CCATGG 1 cut(s) 1583
BspACI CCGC 5 cut(s) 720, 937, 951, 973, 1326
BspANI GGCC 3 cut(s) 75, 203, 602
BspCNI CTCAG 4 cut(s) 229, 264, 556, 1383
BspEI TCCGGA 1 cut(s) 111
BspFNI CGCG 1 cut(s) 1328
BspHI TCATGA 1 cut(s) 1614
BspLI GGNNCC 7 cut(s) 76, 88, 110, 119, 170, 893, 1033
BspMAI CTGCAG 3 cut(s) 692, 791, 1356
BspOI GCTAGC 1 cut(s) 452
BspPI GGATC 1 cut(s) 806
BspT104I TTCGAA 1 cut(s) 1018
BspT107I GGYRCC 4 cut(s) 117, 168, 891, 1031
BspTNI GGTCTC 3 cut(s) 524, 1216, 1482
BsrI ACTGG 3 cut(s) 136, 612, 1610
BssECI CCNNGG 6 cut(s) 597, 648, 660, 772, 895, 1583
BssMI GATC 8 cut(s) 28, 45, 415, 552, 726, 798, 1039, 1098
BssNI GRCGYC 3 cut(s) 118, 1032, 1391
BssT1I CCWWGG 3 cut(s) 648, 660, 1583
Bst2UI CCWGG 4 cut(s) 200, 485, 494, 773
Bst4CI ACNGT 5 cut(s) 310, 877, 929, 989, 1339
Bst6I CTCTTC 3 cut(s) 1199, 1371, 1475
BstACI GRCGYC 3 cut(s) 118, 1032, 1391
BstAPI GCANNNNNTGC 2 cut(s) 786, 945
BstBI TTCGAA 1 cut(s) 1018
BstC8I GCNNGC 9 cut(s) 85, 122, 397, 450, 1265, 1299, 1323, 1330, 1452
BstDEI CTNAG 4 cut(s) 237, 251, 564, 1370
BstDSI CCRYGG 1 cut(s) 1583
BstF5I GGATG 3 cut(s) 537, 1351, 1404
BstFNI CGCG 1 cut(s) 1328
BstH2I RGCGCY 4 cut(s) 121, 795, 1035, 1312
BstHHI GCGC 5 cut(s) 120, 794, 1034, 1311, 1557
BstKTI GATC 8 cut(s) 31, 48, 418, 555, 729, 801, 1042, 1101
BstMAI GTCTC 6 cut(s) 254, 366, 524, 866, 1216, 1482
BstMBI GATC 8 cut(s) 28, 45, 415, 552, 726, 798, 1039, 1098
BstMCI CGRYCG 1 cut(s) 1042
BstMWI GCNNNNNNNGC 4 cut(s) 786, 945, 1090, 1348
BstNI CCWGG 4 cut(s) 200, 485, 494, 773
BstNSI RCATGY 1 cut(s) 829
BstPAI GACNNNNGTC 1 cut(s) 650
BstSCI CCNGG 7 cut(s) 198, 483, 492, 771, 894, 895, 1557
BstSFI CTRYAG 3 cut(s) 688, 787, 1352
BstSLI GKGCMC 1 cut(s) 1323
BstUI CGCG 1 cut(s) 1328
BstV1I GCAGC 6 cut(s) 702, 773, 801, 1198, 1338, 1392
BstV2I GAAGAC 1 cut(s) 328
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
BsuRI GGCC 3 cut(s) 75, 203, 602
BtgI CCRYGG 1 cut(s) 1583
BtsCI GGATG 3 cut(s) 537, 1351, 1404
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 7 cut(s) 238, 306, 555, 561, 570, 605, 1133
Cac8I GCNNGC 9 cut(s) 85, 122, 397, 450, 1265, 1299, 1323, 1330, 1452
CaiI CAGNNNCTG 1 cut(s) 484
CciI TCATGA 1 cut(s) 1614
CfoI GCGC 5 cut(s) 120, 794, 1034, 1311, 1557
Cfr13I GGNCC 5 cut(s) 74, 109, 652, 1160, 1548
Cfr9I CCCGGG 1 cut(s) 895
CseI GACGC 2 cut(s) 1380, 1411
Csp6I GTAC 4 cut(s) 154, 169, 892, 1060
CspCI CAANNNNNGTGG 4 cut(s) 295, 330, 757, 792
CviQI GTAC 4 cut(s) 154, 169, 892, 1060
DdeI CTNAG 4 cut(s) 237, 251, 564, 1370
DinI GGCGCC 2 cut(s) 119, 1033
DpnI GATC 8 cut(s) 30, 47, 417, 554, 728, 800, 1041, 1100
DpnII GATC 8 cut(s) 28, 45, 415, 552, 726, 798, 1039, 1098
DriI GACNNNNNGTC 1 cut(s) 1166
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 3 cut(s) 1199, 1371, 1475
Eam1105I GACNNNNNGTC 1 cut(s) 1166
EarI CTCTTC 3 cut(s) 1199, 1371, 1475
EciI GGCGGA 2 cut(s) 709, 940
Ecl136II GAGCTC 2 cut(s) 634, 1369
Eco130I CCWWGG 3 cut(s) 648, 660, 1583
Eco147I AGGCCT 1 cut(s) 602
Eco24I GRGCYC 2 cut(s) 636, 1371
Eco31I GGTCTC 3 cut(s) 524, 1216, 1482
Eco47I GGWCC 4 cut(s) 109, 652, 1160, 1548
Eco47III AGCGCT 2 cut(s) 793, 1310
Eco53kI GAGCTC 2 cut(s) 634, 1369
Eco57I CTGAAG 2 cut(s) 815, 1033
Eco88I CYCGRG 3 cut(s) 895, 1213, 1364
EcoICRI GAGCTC 2 cut(s) 634, 1369
EcoRI GAATTC 2 cut(s) 488, 501
EcoRII CCWGG 4 cut(s) 198, 483, 492, 771
EcoT14I CCWWGG 3 cut(s) 648, 660, 1583
EcoT38I GRGCYC 2 cut(s) 636, 1371
EgeI GGCGCC 2 cut(s) 119, 1033
EheI GGCGCC 2 cut(s) 119, 1033
ErhI CCWWGG 3 cut(s) 648, 660, 1583
Esp3I CGTCTC 1 cut(s) 866
FaqI GGGAC 2 cut(s) 642, 836
FauNDI CATATG 1 cut(s) 1439
FbaI TGATCA 2 cut(s) 552, 726
FblI GTMKAC 2 cut(s) 351, 1273
Fnu4HI GCNGC 8 cut(s) 691, 787, 790, 973, 1187, 1326, 1352, 1406
FokI GGATG 3 cut(s) 524, 1358, 1411
FriOI GRGCYC 2 cut(s) 636, 1371
Fsp4HI GCNGC 8 cut(s) 691, 787, 790, 973, 1187, 1326, 1352, 1406
FspBI CTAG 7 cut(s) 18, 302, 408, 449, 819, 1112, 1166
GlaI GCGC 5 cut(s) 119, 793, 1033, 1310, 1556
GluI GCNGC 8 cut(s) 691, 787, 790, 973, 1187, 1326, 1352, 1406
GsaI CCCAGC 1 cut(s) 798
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 4 cut(s) 121, 795, 1035, 1312
HaeIII GGCC 3 cut(s) 75, 203, 602
HapII CCGG 5 cut(s) 72, 112, 125, 896, 1558
HgaI GACGC 2 cut(s) 1380, 1411
HhaI GCGC 5 cut(s) 120, 794, 1034, 1311, 1557
Hin1I GRCGYC 3 cut(s) 118, 1032, 1391
Hin6I GCGC 5 cut(s) 118, 792, 1032, 1309, 1555
HinP1I GCGC 5 cut(s) 118, 792, 1032, 1309, 1555
HincII GTYRAC 2 cut(s) 52, 1274
HindII GTYRAC 2 cut(s) 52, 1274
HinfI GANTC 6 cut(s) 60, 65, 247, 1270, 1521, 1579
HpaII CCGG 5 cut(s) 72, 112, 125, 896, 1558
HphI GGTGA 6 cut(s) 154, 622, 694, 874, 1139, 1141
Hpy166II GTNNAC 8 cut(s) 52, 145, 352, 685, 1060, 1241, 1274, 1321
Hpy188I TCNGA 3 cut(s) 620, 1160, 1183
Hpy188III TCNNGA 5 cut(s) 112, 1213, 1286, 1518, 1615
Hpy8I GTNNAC 8 cut(s) 52, 145, 352, 685, 1060, 1241, 1274, 1321
Hpy99I CGWCG 2 cut(s) 1040, 1396
HpyAV CCTTC 5 cut(s) 581, 1181, 1186, 1229, 1581
HpyCH4III ACNGT 5 cut(s) 310, 877, 929, 989, 1339
HpyCH4IV ACGT 2 cut(s) 156, 666
HpyCH4V TGCA 8 cut(s) 575, 690, 789, 1321, 1354, 1408, 1530, 1596
HpyF10VI GCNNNNNNNGC 4 cut(s) 786, 945, 1090, 1348
HpyF3I CTNAG 4 cut(s) 237, 251, 564, 1370
HpySE526I ACGT 2 cut(s) 156, 666
Hsp92I GRCGYC 3 cut(s) 118, 1032, 1391
HspAI GCGC 5 cut(s) 118, 792, 1032, 1309, 1555
KasI GGCGCC 2 cut(s) 117, 1031
Kpn2I TCCGGA 1 cut(s) 111
KpnI GGTACC 2 cut(s) 172, 895
Ksp22I TGATCA 2 cut(s) 552, 726
Kzo9I GATC 8 cut(s) 28, 45, 415, 552, 726, 798, 1039, 1098
LmnI GCTCC 8 cut(s) 92, 262, 377, 433, 620, 953, 1183, 1194
Lsp1109I GCAGC 6 cut(s) 702, 773, 801, 1198, 1338, 1392
LweI GCATC 1 cut(s) 277
MaeI CTAG 7 cut(s) 18, 302, 408, 449, 819, 1112, 1166
MaeII ACGT 2 cut(s) 156, 666
MaeIII GTNAC 2 cut(s) 977, 1418
MalI GATC 8 cut(s) 30, 47, 417, 554, 728, 800, 1041, 1100
MboI GATC 8 cut(s) 28, 45, 415, 552, 726, 798, 1039, 1098
MflI RGATCY 1 cut(s) 28
MhlI GDGCHC 5 cut(s) 625, 636, 869, 1323, 1371
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 5 cut(s) 437, 488, 501, 886, 1618
MluNI TGGCCA 1 cut(s) 203
Mly113I GGCGCC 2 cut(s) 118, 1032
MlyI GAGTC 2 cut(s) 256, 1279
Mox20I TGGCCA 1 cut(s) 203
MroI TCCGGA 1 cut(s) 111
MscI TGGCCA 1 cut(s) 203
Msp20I TGGCCA 1 cut(s) 203
MspI CCGG 5 cut(s) 72, 112, 125, 896, 1558
MspR9I CCNGG 7 cut(s) 200, 485, 494, 773, 896, 897, 1559
Mva1269I GAATGC 1 cut(s) 1301
MvaI CCWGG 4 cut(s) 200, 485, 494, 773
MvnI CGCG 1 cut(s) 1328
MwoI GCNNNNNNNGC 4 cut(s) 786, 945, 1090, 1348
NarI GGCGCC 2 cut(s) 118, 1032
NciI CCSGG 3 cut(s) 896, 897, 1559
NcoI CCATGG 1 cut(s) 1583
NdeI CATATG 1 cut(s) 1439
NdeII GATC 8 cut(s) 28, 45, 415, 552, 726, 798, 1039, 1098
NheI GCTAGC 1 cut(s) 448
NlaIV GGNNCC 7 cut(s) 76, 88, 110, 119, 170, 893, 1033
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 1 cut(s) 977
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 1018
PaeR7I CTCGAG 2 cut(s) 1213, 1364
PagI TCATGA 1 cut(s) 1614
PceI AGGCCT 1 cut(s) 602
PcsI WCGNNNNNNNCGW 2 cut(s) 1011, 1371
PctI GAATGC 1 cut(s) 1301
PfeI GAWTC 4 cut(s) 60, 65, 1521, 1579
PkrI GCNGC 8 cut(s) 692, 788, 791, 974, 1188, 1327, 1353, 1407
Ple19I CGATCG 1 cut(s) 1042
PleI GAGTC 2 cut(s) 255, 1278
PluTI GGCGCC 2 cut(s) 121, 1035
PpsI GAGTC 2 cut(s) 255, 1278
PshAI GACNNNNGTC 1 cut(s) 650
Psp124BI GAGCTC 2 cut(s) 636, 1371
Psp6I CCWGG 4 cut(s) 198, 483, 492, 771
PspFI CCCAGC 1 cut(s) 794
PspGI CCWGG 4 cut(s) 198, 483, 492, 771
PspN4I GGNNCC 7 cut(s) 76, 88, 110, 119, 170, 893, 1033
PspPI GGNCC 5 cut(s) 74, 109, 652, 1160, 1548
PspXI VCTCGAGB 1 cut(s) 1364
PsrI GAACNNNNNNTAC 2 cut(s) 137, 169
PstI CTGCAG 3 cut(s) 692, 791, 1356
PstNI CAGNNNCTG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 28
PvuI CGATCG 1 cut(s) 1042
RsaI GTAC 4 cut(s) 155, 170, 893, 1061
RsaNI GTAC 4 cut(s) 154, 169, 892, 1060
SacI GAGCTC 2 cut(s) 636, 1371
SalI GTCGAC 1 cut(s) 1272
SatI GCNGC 8 cut(s) 691, 787, 790, 973, 1187, 1326, 1352, 1406
Sau3AI GATC 8 cut(s) 28, 45, 415, 552, 726, 798, 1039, 1098
Sau96I GGNCC 5 cut(s) 74, 109, 652, 1160, 1548
SchI GAGTC 2 cut(s) 256, 1279
ScrFI CCNGG 7 cut(s) 200, 485, 494, 773, 896, 897, 1559
SduI GDGCHC 5 cut(s) 625, 636, 869, 1323, 1371
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 3 cut(s) 688, 787, 1352
SfoI GGCGCC 2 cut(s) 119, 1033
Sfr274I CTCGAG 2 cut(s) 1213, 1364
SfuI TTCGAA 1 cut(s) 1018
SinI GGWCC 4 cut(s) 109, 652, 1160, 1548
SlaI CTCGAG 2 cut(s) 1213, 1364
SmaI CCCGGG 1 cut(s) 897
SmlI CTYRAG 4 cut(s) 149, 1085, 1213, 1364
SmoI CTYRAG 4 cut(s) 149, 1085, 1213, 1364
Sse9I AATT 5 cut(s) 437, 488, 501, 886, 1618
SseBI AGGCCT 1 cut(s) 602
SsiI CCGC 5 cut(s) 720, 937, 951, 973, 1326
SspDI GGCGCC 2 cut(s) 117, 1031
SspMI CTAG 7 cut(s) 18, 302, 408, 449, 819, 1112, 1166
SstI GAGCTC 2 cut(s) 636, 1371
StuI AGGCCT 1 cut(s) 602
StyD4I CCNGG 7 cut(s) 198, 483, 492, 771, 894, 895, 1557
StyI CCWWGG 3 cut(s) 648, 660, 1583
TaaI ACNGT 5 cut(s) 310, 877, 929, 989, 1339
TaiI ACGT 2 cut(s) 159, 669
TaqII GACCGA 1 cut(s) 1506
TasI AATT 5 cut(s) 437, 488, 501, 886, 1618
TauI GCSGC 2 cut(s) 975, 1328
TfiI GAWTC 4 cut(s) 60, 65, 1521, 1579
TscAI CASTG 7 cut(s) 238, 313, 555, 568, 577, 612, 1140
TseFI GTSAC 1 cut(s) 977
TseI GCWGC 6 cut(s) 690, 786, 789, 1186, 1351, 1405
Tsp45I GTSAC 1 cut(s) 977
TspDTI ATGAA 3 cut(s) 430, 489, 596
TspGWI ACGGA 2 cut(s) 91, 644
TspMI CCCGGG 1 cut(s) 895
TspRI CASTG 7 cut(s) 238, 313, 555, 568, 577, 612, 1140
VneI GTGCAC 1 cut(s) 1319
VpaK11BI GGWCC 4 cut(s) 109, 652, 1160, 1548
XapI RAATTY 2 cut(s) 488, 501
XceI RCATGY 1 cut(s) 829
XhoI CTCGAG 2 cut(s) 1213, 1364
XmaI CCCGGG 1 cut(s) 895
XmiI GTMKAC 2 cut(s) 351, 1273
XspI CTAG 7 cut(s) 18, 302, 408, 449, 819, 1112, 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.