Rorug06G0334400

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015128
Physical Location & Seq
Forward (+)
48689279 .. 48692067
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0334400.1

Sequence Viewer

Length: 1029 bp
ATGCTACCTTGGGGTGGATTAAGCTGTTGTTTGAGTGCTGCTGCTCTCTATCTTCTCGGCAGAAGCAGTGGCAGGGACGCTGATATTCTTAGGACAGCCACTCGGGTCAGCCAGTTGAAGGAATTGGCTAAATTGTTGGATTCTGAGTCTATATTACCATTGGTGGTTGCGATTTCGGGGAGAGTTAGCTCTGACACTCCCATCACCTGTGAGTTCAGTGGTTTAAGAGGTGTAGTTGTGGAAGAAACGGCAGAACAACATTTTCTGAAACACAATGATGCTGGATCATGGATACAGGATTCTGCTTTGATGCTGTCGATGAGTAAAGAAGTCCCTTGGTATCTGGATGATGGGACTGGTCGCGTGCATGTGGTGGGAGCTCGAGGTGCCACAGGCTTTTCATTGCCGGTTGCAAGTGAGGTATTTGAAGAGTCAGGGCGATCCCTTGTACGAGGGACATTAGATTATCTTCAAGGCCTCAAGATGCTTGGAGTCAAACGAATTGAGAGGGTATTACCAACTGGTACTTCTTTGAGTGTTGTTGGTGAGGCTGTTAAAGATGACATTGGTACCATTAGAATTCAACGACCCCATAAGGGACCATTTTATATCTCCCCCAAAACCATTGATCAGCTTATTGCAAACCTGGGGAAATGGGCAAGGTTGTACAAATATGCATCTTTGGGTTTGAGTGTGTTTGGTGTTTATTTAATTGCTAAGCATTCCGTCCACTACATCATGGAAAGAAAGCGGCGTTGGGAATTACAGAAAAGGGTTCTGGCTGCAGCTGCTAAAAGATCCGGGGAAGATGATGAAGGTTCAATTGAAAGAGATGATAATGCATCAGATGCTCCCAAGAAAGACCGTCTGATGCCTGATTTATGTGTGATATGCCTTGAGCAGGAGTATAATGCGGTTTTTGTCCCGTGTGGTCATATGTGCTGTTGTACAACCTGCTCCTTGCACCTGACCAATTGTCCTCTCTGCCGGCGACGAATTGAGCAGGTGGTTAAGACTTTCCGCCATTAA

Protein Analysis

342

Amino Acids

37.79

Weight (kDa)

8.55

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIDE PF12483 85 - 235 1.6e-41 E3 Ubiquitin ligase
zf-C3HC4_3 PF13920 293 - 336 2.1e-14 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 295 - 335 2.7e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015128)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26250
fragaria_vesca FvH4_2g36990
malus_domestica MD08G1046600.v1.1
prunus_persica Prupe.1G401500_v2.0.a1
pyrus_communis pycom08g03880 pycom15g04560
rosa_chinensis RchiOBHm_Chr6g0304771
rosa_laevigata RLG00000010951
rosa_multiflora Rmu_sc0003120.1_g000005
rosa_roxburghii Rroxscaffold_7G00163510
rosa_rugosa Rorug06G0334400
rosa_samantha Rh6AG446300 Rh6BG455300 Rh6CG459800 Rh6DG446600
rosa_wichuraiana Rw6G038830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 994
Acc36I ACCTGC 2 cut(s) 962, 994
Acc65I GGTACC 1 cut(s) 569
AccB1I GGYRCC 2 cut(s) 386, 569
AccII CGCG 1 cut(s) 363
AciI CCGC 3 cut(s) 751, 914, 1021
AclWI GGATC 3 cut(s) 292, 435, 792
AcsI RAATTY 1 cut(s) 579
AfaI GTAC 5 cut(s) 450, 526, 571, 668, 949
AfiI CCNNNNNNNGG 4 cut(s) 14, 118, 596, 901
AgsI TTSAA 6 cut(s) 118, 428, 473, 584, 822, 827
AhdI GACNNNNNGTC 1 cut(s) 975
AjnI CCWGG 1 cut(s) 645
AjuI GAANNNNNNNTTGG 4 cut(s) 511, 543, 739, 771
AluBI AGCT 5 cut(s) 24, 189, 380, 634, 788
AluI AGCT 5 cut(s) 24, 189, 380, 634, 788
Alw21I GWGCWC 1 cut(s) 382
AlwI GGATC 3 cut(s) 292, 435, 792
Ama87I CYCGRG 2 cut(s) 102, 381
AoxI GGCC 1 cut(s) 475
ApeKI GCWGC 5 cut(s) 38, 41, 782, 785, 788
ApoI RAATTY 1 cut(s) 579
Asp718I GGTACC 1 cut(s) 569
AspS9I GGNCC 1 cut(s) 599
AsuC2I CCSGG 1 cut(s) 802
AsuHPI GGTGA 2 cut(s) 196, 557
AvaI CYCGRG 2 cut(s) 102, 381
AvaII GGWCC 1 cut(s) 599
BaeI ACNNNNGTAYC 2 cut(s) 553, 586
BanI GGYRCC 2 cut(s) 386, 569
BanII GRGCYC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 5 cut(s) 25, 28, 769, 775, 797
BccI CCATC 2 cut(s) 209, 344
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 647
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 1 cut(s) 802
BfmI CTRYAG 1 cut(s) 783
BfuAI ACCTGC 2 cut(s) 962, 994
BfuI GTATCC 1 cut(s) 285
BisI GCNGC 6 cut(s) 39, 42, 752, 783, 786, 789
BlpI GCTNAGC 1 cut(s) 717
BlsI GCNGC 6 cut(s) 40, 43, 753, 784, 787, 790
Bme1390I CCNGG 2 cut(s) 647, 802
Bme18I GGWCC 1 cut(s) 599
BmeRI GACNNNNNGTC 1 cut(s) 975
BmeT110I CYCGRG 2 cut(s) 102, 381
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 3 cut(s) 388, 571, 600
BmrFI CCNGG 2 cut(s) 647, 802
BmsI GCATC 7 cut(s) 268, 300, 474, 686, 838, 851, 861
Bpu1102I GCTNAGC 1 cut(s) 717
BpuEI CTTGAG 2 cut(s) 464, 917
BpuMI CCSGG 1 cut(s) 802
BsaJI CCNNGG 4 cut(s) 8, 335, 646, 801
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 118, 596, 901
Bse118I RCCGGY 2 cut(s) 406, 987
Bse1I ACTGG 3 cut(s) 112, 361, 526
Bse3DI GCAATG 1 cut(s) 401
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 4 cut(s) 8, 335, 646, 801
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 4 cut(s) 14, 118, 596, 901
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 3 cut(s) 112, 361, 526
BseXI GCAGC 5 cut(s) 25, 28, 769, 775, 797
Bsh1236I CGCG 1 cut(s) 363
BshFI GGCC 1 cut(s) 477
BshNI GGYRCC 2 cut(s) 386, 569
BsiHKAI GWGCWC 1 cut(s) 382
BsiHKCI CYCGRG 2 cut(s) 102, 381
BsiSI CCGG 3 cut(s) 407, 801, 988
BslFI GGGAC 6 cut(s) 89, 317, 367, 469, 612, 908
BslI CCNNNNNNNGG 4 cut(s) 14, 118, 596, 901
BsmFI GGGAC 6 cut(s) 89, 317, 367, 469, 612, 908
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 1 cut(s) 477
BsoBI CYCGRG 2 cut(s) 102, 381
Bsp1286I GDGCHC 1 cut(s) 382
Bsp1407I TGTACA 2 cut(s) 666, 947
Bsp143I GATC 4 cut(s) 284, 440, 628, 797
Bsp1720I GCTNAGC 1 cut(s) 717
BspACI CCGC 3 cut(s) 751, 914, 1021
BspANI GGCC 1 cut(s) 477
BspCNI CTCAG 1 cut(s) 136
BspFNI CGCG 1 cut(s) 363
BspLI GGNNCC 3 cut(s) 388, 571, 600
BspMAI CTGCAG 1 cut(s) 787
BspMI ACCTGC 2 cut(s) 962, 994
BspPI GGATC 3 cut(s) 292, 435, 792
BspT107I GGYRCC 2 cut(s) 386, 569
BsrDI GCAATG 1 cut(s) 401
BsrFI RCCGGY 2 cut(s) 406, 987
BsrGI TGTACA 2 cut(s) 666, 947
BsrI ACTGG 3 cut(s) 112, 361, 526
BssAI RCCGGY 2 cut(s) 406, 987
BssECI CCNNGG 4 cut(s) 8, 335, 646, 801
BssMI GATC 4 cut(s) 284, 440, 628, 797
BssT1I CCWWGG 2 cut(s) 8, 335
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 1 cut(s) 866
Bst6I CTCTTC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 848
BstAUI TGTACA 2 cut(s) 666, 947
BstC8I GCNNGC 2 cut(s) 365, 989
BstDEI CTNAG 3 cut(s) 89, 144, 717
BstENI CCTNNNNNAGG 1 cut(s) 899
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 1 cut(s) 363
BstKTI GATC 4 cut(s) 287, 443, 631, 800
BstMBI GATC 4 cut(s) 284, 440, 628, 797
BstMWI GCNNNNNNNGC 3 cut(s) 386, 788, 848
BstNI CCWGG 1 cut(s) 647
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 645, 800
BstSFI CTRYAG 1 cut(s) 783
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 5 cut(s) 25, 28, 769, 775, 797
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 1 cut(s) 477
BtsCI GGATG 1 cut(s) 352
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 2 cut(s) 73, 223
BveI ACCTGC 2 cut(s) 962, 994
Cac8I GCNNGC 2 cut(s) 365, 989
Cfr10I RCCGGY 2 cut(s) 406, 987
Cfr13I GGNCC 1 cut(s) 599
CseI GACGC 1 cut(s) 86
Csp6I GTAC 5 cut(s) 449, 525, 570, 667, 948
CviAII CATG 3 cut(s) 288, 368, 739
CviQI GTAC 5 cut(s) 449, 525, 570, 667, 948
DdeI CTNAG 3 cut(s) 89, 144, 717
DpnI GATC 4 cut(s) 286, 442, 630, 799
DpnII GATC 4 cut(s) 284, 440, 628, 797
DriI GACNNNNNGTC 1 cut(s) 975
Eam1104I CTCTTC 1 cut(s) 423
Eam1105I GACNNNNNGTC 1 cut(s) 975
EarI CTCTTC 1 cut(s) 423
EciI GGCGGA 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 8, 335
Eco147I AGGCCT 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 599
Eco53kI GAGCTC 1 cut(s) 380
Eco88I CYCGRG 2 cut(s) 102, 381
EcoICRI GAGCTC 1 cut(s) 380
EcoNI CCTNNNNNAGG 1 cut(s) 899
EcoRI GAATTC 1 cut(s) 579
EcoRII CCWGG 1 cut(s) 645
EcoT14I CCWWGG 2 cut(s) 8, 335
EcoT22I ATGCAT 2 cut(s) 679, 844
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 2 cut(s) 8, 335
FaeI CATG 3 cut(s) 291, 371, 742
FaqI GGGAC 6 cut(s) 89, 317, 367, 469, 612, 908
FatI CATG 3 cut(s) 287, 367, 738
FauNDI CATATG 1 cut(s) 936
FbaI TGATCA 1 cut(s) 628
Fnu4HI GCNGC 6 cut(s) 39, 42, 752, 783, 786, 789
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 6 cut(s) 39, 42, 752, 783, 786, 789
GluI GCNGC 6 cut(s) 39, 42, 752, 783, 786, 789
HaeIII GGCC 1 cut(s) 477
HapII CCGG 3 cut(s) 407, 801, 988
HgaI GACGC 1 cut(s) 86
Hin1II CATG 3 cut(s) 291, 371, 742
HinfI GANTC 5 cut(s) 140, 146, 299, 431, 492
HpaII CCGG 3 cut(s) 407, 801, 988
HphI GGTGA 2 cut(s) 196, 557
Hpy166II GTNNAC 1 cut(s) 730
Hpy188I TCNGA 5 cut(s) 145, 193, 267, 847, 870
Hpy188III TCNNGA 2 cut(s) 344, 481
Hpy8I GTNNAC 1 cut(s) 730
Hpy99I CGWCG 1 cut(s) 996
HpyAV CCTTC 2 cut(s) 112, 809
HpyCH4III ACNGT 1 cut(s) 866
HpyCH4V TGCA 7 cut(s) 367, 413, 641, 677, 785, 842, 964
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 788, 848
HpyF3I CTNAG 3 cut(s) 89, 144, 717
Hsp92II CATG 3 cut(s) 291, 371, 742
KpnI GGTACC 1 cut(s) 573
KroI GCCGGC 1 cut(s) 987
KroNI GCCGGC 1 cut(s) 989
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 4 cut(s) 284, 440, 628, 797
LmnI GCTCC 3 cut(s) 377, 856, 962
Lsp1109I GCAGC 5 cut(s) 25, 28, 769, 775, 797
LweI GCATC 7 cut(s) 268, 300, 474, 686, 838, 851, 861
MalI GATC 4 cut(s) 286, 442, 630, 799
MboI GATC 4 cut(s) 284, 440, 628, 797
MboII GAAGA 5 cut(s) 44, 254, 440, 461, 818
MfeI CAATTG 2 cut(s) 822, 973
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 9 cut(s) 122, 131, 501, 579, 711, 761, 822, 973, 996
MlyI GAGTC 3 cut(s) 155, 440, 501
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 8 cut(s) 221, 377, 412, 446, 488, 501, 541, 990
Mph1103I ATGCAT 2 cut(s) 679, 844
MroNI GCCGGC 1 cut(s) 987
MseI TTAA 6 cut(s) 20, 224, 555, 710, 1011, 1027
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 3 cut(s) 407, 801, 988
MspR9I CCNGG 2 cut(s) 647, 802
MunI CAATTG 2 cut(s) 822, 973
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 1 cut(s) 647
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 3 cut(s) 386, 788, 848
NaeI GCCGGC 1 cut(s) 989
NciI CCSGG 1 cut(s) 802
NdeI CATATG 1 cut(s) 936
NdeII GATC 4 cut(s) 284, 440, 628, 797
NgoMIV GCCGGC 1 cut(s) 987
NlaIII CATG 3 cut(s) 291, 371, 742
NlaIV GGNNCC 3 cut(s) 388, 571, 600
NmeAIII GCCGAG 1 cut(s) 36
NsiI ATGCAT 2 cut(s) 679, 844
NspI RCATGY 1 cut(s) 371
PaeR7I CTCGAG 1 cut(s) 381
PaqCI CACCTGC 1 cut(s) 994
PceI AGGCCT 1 cut(s) 477
PctI GAATGC 1 cut(s) 721
PdiI GCCGGC 1 cut(s) 989
PfeI GAWTC 2 cut(s) 140, 299
PkrI GCNGC 6 cut(s) 40, 43, 753, 784, 787, 790
PleI GAGTC 3 cut(s) 154, 439, 500
PpsI GAGTC 3 cut(s) 154, 439, 500
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 3 cut(s) 388, 571, 600
PspPI GGNCC 1 cut(s) 599
PspXI VCTCGAGB 1 cut(s) 381
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 5 cut(s) 450, 526, 571, 668, 949
RsaNI GTAC 5 cut(s) 449, 525, 570, 667, 948
RseI CAYNNNNRTG 1 cut(s) 276
SacI GAGCTC 1 cut(s) 382
SaqAI TTAA 6 cut(s) 20, 224, 555, 710, 1011, 1027
SatI GCNGC 6 cut(s) 39, 42, 752, 783, 786, 789
Sau3AI GATC 4 cut(s) 284, 440, 628, 797
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 3 cut(s) 155, 440, 501
ScrFI CCNGG 2 cut(s) 647, 802
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 7 cut(s) 268, 300, 474, 686, 838, 851, 861
SfcI CTRYAG 1 cut(s) 783
Sfr274I CTCGAG 1 cut(s) 381
SinI GGWCC 1 cut(s) 599
SlaI CTCGAG 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 3 cut(s) 381, 479, 896
SmoI CTYRAG 3 cut(s) 381, 479, 896
Sse9I AATT 9 cut(s) 122, 131, 501, 579, 711, 761, 822, 973, 996
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 3 cut(s) 751, 914, 1021
SstI GAGCTC 1 cut(s) 382
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 2 cut(s) 645, 800
StyI CCWWGG 2 cut(s) 8, 335
TaaI ACNGT 1 cut(s) 866
TaqI TCGA 2 cut(s) 317, 382
TasI AATT 9 cut(s) 122, 131, 501, 579, 711, 761, 822, 973, 996
TatI WGTACW 2 cut(s) 666, 947
TauI GCSGC 1 cut(s) 754
TfiI GAWTC 2 cut(s) 140, 299
Tru1I TTAA 6 cut(s) 20, 224, 555, 710, 1011, 1027
Tru9I TTAA 6 cut(s) 20, 224, 555, 710, 1011, 1027
TscAI CASTG 2 cut(s) 73, 223
TseI GCWGC 5 cut(s) 38, 41, 782, 785, 788
TspDTI ATGAA 2 cut(s) 390, 828
TspGWI ACGGA 1 cut(s) 715
TspRI CASTG 2 cut(s) 73, 223
VpaK11BI GGWCC 1 cut(s) 599
XagI CCTNNNNNAGG 1 cut(s) 899
XapI RAATTY 1 cut(s) 579
XceI RCATGY 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 381
Zsp2I ATGCAT 2 cut(s) 679, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.