MD08G1046600.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015128
Physical Location & Seq
Forward (+)
3522923 .. 3526496
3574 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1046600.v1.1.491

Sequence Viewer

Length: 1626 bp
ATGCCAATCCCATCCACCATGGCCAGCGAGCAAGATCTTCTGTCCACGGAGATTGTCAACCGTGGGGTTGAGTCGTCCGGCCCCAATGCCGGCTCCCTCACATTCTCAGTGAGGGTGCAAAGGCGGCTGCCGGACTTTTTACAGTCCGTAAATCTAAAGTATGTGAAGTTGGGGTACCACCACCTCATCAACCATGCCATATATTTAGCAACAATCCCTGTGCTTGTGCTGGTGTTCAGTGCTGAGGTGGGAAGTTTGAGCAGGGAGGAGCTGTGGAAGAAGCTTTGGGATGATGGACGTTATGACCTAGCTACTGTGCTAGGGTTCTTTGGTGTATTTGTTTTCACTTTGTCAGTTTACTTCATGTCACGCCCTCGATCCATCTACCTCGTTGATTTCGCTTGTTTTCAGCCTAATGACGATCTGAAGGCCTCTAAGGAGCAATTCATAGAGGTAGCAAGAAAATCAGGCAAATTCAACGAGGCGAGCCTAGAATTCCAGAAGAGAATTCTAAACTCATCAGGCTTAGGAGACGAGACCTACATCCCAAAATCGGTCATCATGTCGACTGAAAACACGGCAACCATGAAAGAAGGCCGAGCTGAGGCCTCAATGGTCATGTTTGGGGCCCTCGACGAGCTCTTTGAAAAGACTAGGGTCCGTCCGAAGGACGTAGGAGTCCTAGTAGTAAATTGTAGCATCTTCAACCCCACACCCTCTTTGTCTGCAATGATCATAAATCACTACAAAATGAGAGGCAACATTTTGAGCTACAATTTGGGTGGAATGGGATGCAGTGCAGGGATCATAGCCGTTGATCTGGCTAGAGACATTCTTCAAGCCAACCCTAATAACTATGCTGTTGTGGTGAGTACTGAGTGTGTGGGGTATAATTGGTACCCTGGTAGTGACAGGTCTATGCTCGTTGCGAATTGCTTTTTCCGCATGGGATGCTCCGCTGTGTTGCTCTCCAATCGCCGCCGCGATTATCACCGTGCTAAGTACCGGCTTGAACACGTTGTCCGGACACATAAAGGCGCTGATGATCGGAGTTTCAGGTGTGTGTACCAAGAGGAAGACGAAAAAGGATTGAAGGGGCTGAAGATCAGCAAAGACCTAATTGAAATTGGTGGTGATGCCTTGAAAACCAACATCACCACTTTGGGGCCGTTAGTGCTGCCTTTCTCTGAGCAGCTCCTCTTCTTTTCCACATTAGTTTCAAGGCACCTGTTTTCCAGCGAAGGCACTTCGGAGTTATCTTTAGCGTCACGCAAGAGACCCTACATTCCGGACTTCAAGCTGGCTTTCGAGGGCTTTTGCGTGCATGCAGCGAGCAAGACCGTGCTCGACGAGCTGCAGAGGAACCTCGAGCTGAGTGAGGAAAACATGGAGGCCTCTAGGATGACGCTACACCGCTTTGGAAACACGTCGAGCAGCAGCATATGGTATGAGTTGGCTTATTTGGAAGCAAAAGAGAAGGTGAAGAGAGGAGATAGGGTTTGGCAGTTGTCATTTGGTTCTGGATTCAAATGCAACAGCATTGTTTGGAGGTCAATGCGCCGCGTTAGGAAGCCCTCGAGGAACCCATGGCTTGACTGCATTGAACGATACCCAGTGAATCTCTAA

Protein Analysis

542

Amino Acids

61.16

Weight (kDa)

8.9

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 120 - 409 5e-132 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 439 - 517 7.6e-13 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 439 - 519 4e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015128)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26250
fragaria_vesca FvH4_2g36990
malus_domestica MD08G1046600.v1.1
prunus_persica Prupe.1G401500_v2.0.a1
pyrus_communis pycom08g03880 pycom15g04560
rosa_chinensis RchiOBHm_Chr6g0304771
rosa_laevigata RLG00000010951
rosa_multiflora Rmu_sc0003120.1_g000005
rosa_roxburghii Rroxscaffold_7G00163510
rosa_rugosa Rorug06G0334400
rosa_samantha Rh6AG446300 Rh6BG455300 Rh6CG459800 Rh6DG446600
rosa_wichuraiana Rw6G038830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 677
AbsI CCTCGAGG 1 cut(s) 1576
Acc65I GGTACC 2 cut(s) 174, 897
AccB1I GGYRCC 3 cut(s) 174, 897, 1224
AccI GTMKAC 1 cut(s) 566
AccII CGCG 2 cut(s) 984, 1563
AccIII TCCGGA 2 cut(s) 1023, 1288
AciI CCGC 7 cut(s) 124, 943, 957, 979, 982, 1414, 1561
AclWI GGATC 2 cut(s) 372, 812
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 3 cut(s) 473, 494, 507
AcuI CTGAAG 2 cut(s) 446, 1121
AfaI GTAC 5 cut(s) 176, 874, 899, 1004, 1067
AfiI CCNNNNNNNGG 5 cut(s) 89, 553, 604, 667, 1164
AflIII ACRYGT 2 cut(s) 1015, 1425
AjiI CACGTC 1 cut(s) 1428
AjnI CCWGG 1 cut(s) 901
AloI GAACNNNNNNTCC 2 cut(s) 1005, 1037
Alw21I GWGCWC 2 cut(s) 642, 1347
Alw26I GTCTC 4 cut(s) 525, 530, 822, 1270
AlwI GGATC 2 cut(s) 372, 812
Ama87I CYCGRG 2 cut(s) 1367, 1576
Aor13HI TCCGGA 2 cut(s) 1023, 1288
AoxI GGCC 8 cut(s) 21, 79, 429, 595, 606, 627, 1166, 1392
ApaI GGGCCC 1 cut(s) 631
ApeKI GCWGC 7 cut(s) 127, 1177, 1192, 1328, 1354, 1434, 1437
ApoI RAATTY 3 cut(s) 473, 494, 507
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
Asp718I GGTACC 2 cut(s) 174, 897
AspLEI GCGC 2 cut(s) 1040, 1560
AspS9I GGNCC 5 cut(s) 80, 627, 628, 658, 1166
AsuHPI GGTGA 5 cut(s) 880, 983, 1145, 1147, 1492
AvaI CYCGRG 2 cut(s) 1367, 1576
AvaII GGWCC 1 cut(s) 658
BaeGI GKGCMC 1 cut(s) 631
BaeI ACNNNNGTAYC 2 cut(s) 889, 922
BalI TGGCCA 1 cut(s) 23
BanI GGYRCC 3 cut(s) 174, 897, 1224
BanII GRGCYC 2 cut(s) 631, 642
BarI GAAGNNNNNNTAC 2 cut(s) 158, 190
BbsI GAAGAC 1 cut(s) 1083
Bbv12I GWGCWC 2 cut(s) 642, 1347
BbvCI CCTCAGC 2 cut(s) 243, 603
BbvI GCAGC 7 cut(s) 114, 1164, 1204, 1340, 1341, 1446, 1449
BccI CCATC 3 cut(s) 19, 287, 389
BceAI ACGGC 3 cut(s) 594, 797, 1153
BciT130I CCWGG 1 cut(s) 903
BclI TGATCA 1 cut(s) 732
BcoDI GTCTC 4 cut(s) 525, 530, 822, 1270
BfaI CTAG 7 cut(s) 308, 320, 491, 654, 683, 825, 1398
BfmI CTRYAG 1 cut(s) 1355
BfoI RGCGCY 1 cut(s) 1041
BglII AGATCT 1 cut(s) 34
BmcAI AGTACT 1 cut(s) 874
Bme1390I CCNGG 1 cut(s) 903
Bme18I GGWCC 1 cut(s) 658
BmeT110I CYCGRG 2 cut(s) 1367, 1576
BmgBI CACGTC 1 cut(s) 1428
BmgT120I GGNCC 5 cut(s) 80, 627, 628, 658, 1166
BmrFI CCNGG 1 cut(s) 903
BmrI ACTGGG 1 cut(s) 1607
BmsI GCATC 4 cut(s) 708, 782, 941, 1126
BmuI ACTGGG 1 cut(s) 1607
BoxI GACNNNNGTC 1 cut(s) 656
BpiI GAAGAC 1 cut(s) 1083
Bpu10I CCTNAGC 3 cut(s) 243, 526, 603
BsaI GGTCTC 2 cut(s) 530, 1270
BsaJI CCNNGG 5 cut(s) 18, 45, 61, 901, 1586
BsaWI WCCGGW 2 cut(s) 1023, 1288
BsaXI ACNNNNNCTCC 6 cut(s) 257, 287, 669, 699, 1482, 1512
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 553, 604, 667, 1164
Bse118I RCCGGY 2 cut(s) 89, 1005
Bse1I ACTGG 1 cut(s) 1613
Bse3DI GCAATG 1 cut(s) 735
BseAI TCCGGA 2 cut(s) 1023, 1288
BseBI CCWGG 1 cut(s) 903
BseDI CCNNGG 5 cut(s) 18, 45, 61, 901, 1586
BseGI GGATG 6 cut(s) 11, 295, 543, 797, 956, 1407
BseLI CCNNNNNNNGG 5 cut(s) 89, 553, 604, 667, 1164
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 6 cut(s) 120, 234, 594, 867, 1179, 1364
BseNI ACTGG 1 cut(s) 1613
BseRI GAGGAG 3 cut(s) 281, 1187, 1503
BseSI GKGCMC 1 cut(s) 631
BseXI GCAGC 7 cut(s) 114, 1164, 1204, 1340, 1341, 1446, 1449
BsgI GTGCAG 1 cut(s) 819
Bsh1236I CGCG 2 cut(s) 984, 1563
BshFI GGCC 8 cut(s) 23, 81, 431, 597, 608, 629, 1168, 1394
BshNI GGYRCC 3 cut(s) 174, 897, 1224
BsiHKAI GWGCWC 2 cut(s) 642, 1347
BsiHKCI CYCGRG 2 cut(s) 1367, 1576
BsiSI CCGG 6 cut(s) 78, 90, 131, 1006, 1024, 1289
BslI CCNNNNNNNGG 5 cut(s) 89, 553, 604, 667, 1164
BsmAI GTCTC 4 cut(s) 525, 530, 822, 1270
BsmBI CGTCTC 1 cut(s) 525
BsnI GGCC 8 cut(s) 23, 81, 431, 597, 608, 629, 1168, 1394
Bso31I GGTCTC 2 cut(s) 530, 1270
BsoBI CYCGRG 2 cut(s) 1367, 1576
Bsp120I GGGCCC 1 cut(s) 627
Bsp1286I GDGCHC 3 cut(s) 631, 642, 1347
Bsp13I TCCGGA 2 cut(s) 1023, 1288
Bsp143I GATC 8 cut(s) 34, 377, 421, 732, 804, 817, 1045, 1104
Bsp19I CCATGG 2 cut(s) 18, 1586
BspACI CCGC 7 cut(s) 124, 943, 957, 979, 982, 1414, 1561
BspANI GGCC 8 cut(s) 23, 81, 431, 597, 608, 629, 1168, 1394
BspCNI CTCAG 6 cut(s) 119, 235, 595, 868, 1180, 1365
BspEI TCCGGA 2 cut(s) 1023, 1288
BspFNI CGCG 2 cut(s) 984, 1563
BspMAI CTGCAG 1 cut(s) 1359
BspPI GGATC 2 cut(s) 372, 812
BspT107I GGYRCC 3 cut(s) 174, 897, 1224
BspTNI GGTCTC 2 cut(s) 530, 1270
BsrDI GCAATG 1 cut(s) 735
BsrFI RCCGGY 2 cut(s) 89, 1005
BsrI ACTGG 1 cut(s) 1613
BssAI RCCGGY 2 cut(s) 89, 1005
BssECI CCNNGG 5 cut(s) 18, 45, 61, 901, 1586
BssMI GATC 8 cut(s) 34, 377, 421, 732, 804, 817, 1045, 1104
BssT1I CCWWGG 2 cut(s) 18, 1586
Bst2UI CCWGG 1 cut(s) 903
Bst4CI ACNGT 5 cut(s) 62, 144, 316, 995, 1342
Bst6I CTCTTC 3 cut(s) 497, 1205, 1478
BstAPI GCANNNNNTGC 1 cut(s) 951
BstC8I GCNNGC 8 cut(s) 25, 29, 91, 487, 1302, 1322, 1326, 1333
BstDEI CTNAG 9 cut(s) 106, 243, 435, 526, 603, 876, 999, 1188, 1373
BstDSI CCRYGG 4 cut(s) 18, 45, 61, 1586
BstF5I GGATG 6 cut(s) 11, 295, 543, 797, 956, 1407
BstFNI CGCG 2 cut(s) 984, 1563
BstH2I RGCGCY 1 cut(s) 1041
BstHHI GCGC 2 cut(s) 1040, 1560
BstKTI GATC 8 cut(s) 37, 380, 424, 735, 807, 820, 1048, 1107
BstMAI GTCTC 4 cut(s) 525, 530, 822, 1270
BstMBI GATC 8 cut(s) 34, 377, 421, 732, 804, 817, 1045, 1104
BstMWI GCNNNNNNNGC 5 cut(s) 124, 942, 951, 1174, 1351
BstNI CCWGG 1 cut(s) 903
BstNSI RCATGY 1 cut(s) 1328
BstPAI GACNNNNGTC 1 cut(s) 656
BstSCI CCNGG 1 cut(s) 901
BstSFI CTRYAG 1 cut(s) 1355
BstSLI GKGCMC 1 cut(s) 631
BstUI CGCG 2 cut(s) 984, 1563
BstV1I GCAGC 7 cut(s) 114, 1164, 1204, 1340, 1341, 1446, 1449
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuRI GGCC 8 cut(s) 23, 81, 431, 597, 608, 629, 1168, 1394
BtgI CCRYGG 4 cut(s) 18, 45, 61, 1586
BtrI CACGTC 1 cut(s) 1428
BtsCI GGATG 6 cut(s) 11, 295, 543, 797, 956, 1407
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 4 cut(s) 114, 244, 802, 1620
Cac8I GCNNGC 8 cut(s) 25, 29, 91, 487, 1302, 1322, 1326, 1333
CfoI GCGC 2 cut(s) 1040, 1560
Cfr10I RCCGGY 2 cut(s) 89, 1005
Cfr13I GGNCC 5 cut(s) 80, 627, 628, 658, 1166
CseI GACGC 2 cut(s) 1254, 1414
Csp6I GTAC 5 cut(s) 175, 873, 898, 1003, 1066
CspCI CAANNNNNGTGG 4 cut(s) 34, 69, 763, 798
CviQI GTAC 5 cut(s) 175, 873, 898, 1003, 1066
DdeI CTNAG 9 cut(s) 106, 243, 435, 526, 603, 876, 999, 1188, 1373
DpnI GATC 8 cut(s) 36, 379, 423, 734, 806, 819, 1047, 1106
DpnII GATC 8 cut(s) 34, 377, 421, 732, 804, 817, 1045, 1104
DrdI GACNNNNNNGTC 1 cut(s) 677
DseDI GACNNNNNNGTC 1 cut(s) 677
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 3 cut(s) 497, 1205, 1478
EarI CTCTTC 3 cut(s) 497, 1205, 1478
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 2 cut(s) 18, 1586
Eco147I AGGCCT 3 cut(s) 431, 608, 1394
Eco24I GRGCYC 2 cut(s) 631, 642
Eco31I GGTCTC 2 cut(s) 530, 1270
Eco47I GGWCC 1 cut(s) 658
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 2 cut(s) 446, 1121
Eco88I CYCGRG 2 cut(s) 1367, 1576
EcoICRI GAGCTC 1 cut(s) 640
EcoO109I RGGNCCY 2 cut(s) 627, 628
EcoRI GAATTC 2 cut(s) 494, 507
EcoRII CCWGG 1 cut(s) 901
EcoT14I CCWWGG 2 cut(s) 18, 1586
EcoT38I GRGCYC 2 cut(s) 631, 642
ErhI CCWWGG 2 cut(s) 18, 1586
Esp3I CGTCTC 1 cut(s) 525
FauNDI CATATG 1 cut(s) 1442
FbaI TGATCA 1 cut(s) 732
FblI GTMKAC 1 cut(s) 566
FokI GGATG 5 cut(s) 302, 530, 804, 963, 1414
FriOI GRGCYC 2 cut(s) 631, 642
FspBI CTAG 7 cut(s) 308, 320, 491, 654, 683, 825, 1398
GlaI GCGC 2 cut(s) 1039, 1559
HaeII RGCGCY 1 cut(s) 1041
HaeIII GGCC 8 cut(s) 23, 81, 431, 597, 608, 629, 1168, 1394
HapII CCGG 6 cut(s) 78, 90, 131, 1006, 1024, 1289
HgaI GACGC 2 cut(s) 1254, 1414
HhaI GCGC 2 cut(s) 1040, 1560
Hin6I GCGC 2 cut(s) 1038, 1558
HinP1I GCGC 2 cut(s) 1038, 1558
HincII GTYRAC 2 cut(s) 58, 567
HindII GTYRAC 2 cut(s) 58, 567
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 4 cut(s) 71, 678, 1524, 1618
HpaII CCGG 6 cut(s) 78, 90, 131, 1006, 1024, 1289
HphI GGTGA 5 cut(s) 880, 983, 1145, 1147, 1492
Hpy166II GTNNAC 5 cut(s) 45, 58, 358, 567, 1066
Hpy188I TCNGA 5 cut(s) 426, 666, 1050, 1189, 1252
Hpy188III TCNNGA 4 cut(s) 499, 1024, 1289, 1521
Hpy8I GTNNAC 5 cut(s) 45, 58, 358, 567, 1066
Hpy99I CGWCG 3 cut(s) 638, 1352, 1432
HpyAV CCTTC 6 cut(s) 421, 587, 661, 1087, 1235, 1471
HpyCH4III ACNGT 5 cut(s) 62, 144, 316, 995, 1342
HpyCH4IV ACGT 4 cut(s) 298, 672, 1017, 1427
HpyCH4V TGCA 9 cut(s) 118, 728, 795, 800, 1324, 1328, 1357, 1533, 1599
HpyF10VI GCNNNNNNNGC 5 cut(s) 124, 942, 951, 1174, 1351
HpyF3I CTNAG 9 cut(s) 106, 243, 435, 526, 603, 876, 999, 1188, 1373
HpySE526I ACGT 4 cut(s) 298, 672, 1017, 1427
HspAI GCGC 2 cut(s) 1038, 1558
Kpn2I TCCGGA 2 cut(s) 1023, 1288
KpnI GGTACC 2 cut(s) 178, 901
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 732
Kzo9I GATC 8 cut(s) 34, 377, 421, 732, 804, 817, 1045, 1104
LmnI GCTCC 5 cut(s) 98, 268, 439, 959, 1200
Lsp1109I GCAGC 7 cut(s) 114, 1164, 1204, 1340, 1341, 1446, 1449
LweI GCATC 4 cut(s) 708, 782, 941, 1126
MaeI CTAG 7 cut(s) 308, 320, 491, 654, 683, 825, 1398
MaeII ACGT 4 cut(s) 298, 672, 1017, 1427
MaeIII GTNAC 3 cut(s) 366, 908, 1266
MalI GATC 8 cut(s) 36, 379, 423, 734, 806, 819, 1047, 1106
MboI GATC 8 cut(s) 34, 377, 421, 732, 804, 817, 1045, 1104
MboII GAAGA 9 cut(s) 29, 289, 514, 694, 827, 1088, 1114, 1192, 1495
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 3 cut(s) 631, 642, 1347
MlsI TGGCCA 1 cut(s) 23
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 2 cut(s) 80, 687
Mox20I TGGCCA 1 cut(s) 23
MroI TCCGGA 2 cut(s) 1023, 1288
MroNI GCCGGC 1 cut(s) 89
MscI TGGCCA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 6 cut(s) 78, 90, 131, 1006, 1024, 1289
MspR9I CCNGG 1 cut(s) 903
MvaI CCWGG 1 cut(s) 903
MvnI CGCG 2 cut(s) 984, 1563
MwoI GCNNNNNNNGC 5 cut(s) 124, 942, 951, 1174, 1351
NaeI GCCGGC 1 cut(s) 91
NcoI CCATGG 2 cut(s) 18, 1586
NdeI CATATG 1 cut(s) 1442
NdeII GATC 8 cut(s) 34, 377, 421, 732, 804, 817, 1045, 1104
NgoMIV GCCGGC 1 cut(s) 89
NmeAIII GCCGAG 1 cut(s) 623
NmuCI GTSAC 3 cut(s) 366, 908, 1266
NspI RCATGY 1 cut(s) 1328
PaeI GCATGC 1 cut(s) 1328
PaeR7I CTCGAG 2 cut(s) 1367, 1576
PceI AGGCCT 3 cut(s) 431, 608, 1394
PdiI GCCGGC 1 cut(s) 91
PfeI GAWTC 2 cut(s) 1524, 1618
PleI GAGTC 2 cut(s) 79, 686
PpsI GAGTC 2 cut(s) 79, 686
PshAI GACNNNNGTC 1 cut(s) 656
Psp124BI GAGCTC 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 901
PspGI CCWGG 1 cut(s) 901
PspOMI GGGCCC 1 cut(s) 627
PspPI GGNCC 5 cut(s) 80, 627, 628, 658, 1166
PspXI VCTCGAGB 2 cut(s) 1367, 1576
PstI CTGCAG 1 cut(s) 1359
PsuI RGATCY 1 cut(s) 34
RsaI GTAC 5 cut(s) 176, 874, 899, 1004, 1067
RsaNI GTAC 5 cut(s) 175, 873, 898, 1003, 1066
SacI GAGCTC 1 cut(s) 642
SalI GTCGAC 1 cut(s) 565
Sau3AI GATC 8 cut(s) 34, 377, 421, 732, 804, 817, 1045, 1104
Sau96I GGNCC 5 cut(s) 80, 627, 628, 658, 1166
ScaI AGTACT 1 cut(s) 874
SchI GAGTC 2 cut(s) 80, 687
ScrFI CCNGG 1 cut(s) 903
SduI GDGCHC 3 cut(s) 631, 642, 1347
SfaNI GCATC 4 cut(s) 708, 782, 941, 1126
SfcI CTRYAG 1 cut(s) 1355
Sfr274I CTCGAG 2 cut(s) 1367, 1576
SinI GGWCC 1 cut(s) 658
SlaI CTCGAG 2 cut(s) 1367, 1576
SmlI CTYRAG 2 cut(s) 1367, 1576
SmoI CTYRAG 2 cut(s) 1367, 1576
SphI GCATGC 1 cut(s) 1328
SseBI AGGCCT 3 cut(s) 431, 608, 1394
SsiI CCGC 7 cut(s) 124, 943, 957, 979, 982, 1414, 1561
SspMI CTAG 7 cut(s) 308, 320, 491, 654, 683, 825, 1398
SstI GAGCTC 1 cut(s) 642
StuI AGGCCT 3 cut(s) 431, 608, 1394
StyD4I CCNGG 1 cut(s) 901
StyI CCWWGG 2 cut(s) 18, 1586
TaaI ACNGT 5 cut(s) 62, 144, 316, 995, 1342
TaiI ACGT 4 cut(s) 301, 675, 1020, 1430
TaqI TCGA 8 cut(s) 376, 566, 633, 1308, 1347, 1368, 1430, 1577
TaqII GACCGA 1 cut(s) 544
TatI WGTACW 1 cut(s) 872
TauI GCSGC 4 cut(s) 127, 981, 984, 1563
TfiI GAWTC 2 cut(s) 1524, 1618
TscAI CASTG 4 cut(s) 114, 244, 802, 1620
TseFI GTSAC 3 cut(s) 366, 908, 1266
TseI GCWGC 7 cut(s) 127, 1177, 1192, 1328, 1354, 1434, 1437
Tsp45I GTSAC 3 cut(s) 366, 908, 1266
TspDTI ATGAA 3 cut(s) 352, 436, 602
TspGWI ACGGA 3 cut(s) 62, 136, 650
TspRI CASTG 4 cut(s) 114, 244, 802, 1620
VpaK11BI GGWCC 1 cut(s) 658
XapI RAATTY 3 cut(s) 473, 494, 507
XceI RCATGY 1 cut(s) 1328
XhoI CTCGAG 2 cut(s) 1367, 1576
XmiI GTMKAC 1 cut(s) 566
XspI CTAG 7 cut(s) 308, 320, 491, 654, 683, 825, 1398
ZrmI AGTACT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.