FvH4_3g00570

Glucan endo-13-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011532
Physical Location & Seq
Forward (+)
289939 .. 290782
844 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00570.t1

Sequence Viewer

Length: 354 bp
ATGCCATCTAGTATTCTGTGGCTTCTGCTAGCTCTGCTGTGTTTAGCAGTAACGCCACGAAGATCTGGTGGACAGTTAGAGGAATGGTGCATAGCGGATGAGCAGACACCGGCGGACGAGCTAAAGATGGCACTTGATTGGGCTTGTGAGAAGGGAGGTGCAGACTGCAGTAAGATACAAGAGAACCAAGCTTGCTACTTGCCTAATACACTGGAGCACCATGCCTCTTATGCTTTCAACAATTACTATCAAAAGTTCAAGCAGGAAGGCGCAACATGTTACTTCAATGCTGCTGCATTTGTCACTGCCCTTGATCCAAGTCATAACTCTTGCAAGTTTGAGTATGTTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
X8

Protein Analysis

118

Amino Acids

13.12

Weight (kDa)

4.86

Isoelectric Point (pI)

55.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 29 - 99 2.3e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 95, 113
AclWI GGATC 1 cut(s) 308
AflIII ACRYGT 1 cut(s) 275
AgsI TTSAA 3 cut(s) 238, 259, 286
AluBI AGCT 3 cut(s) 32, 121, 191
AluI AGCT 3 cut(s) 32, 121, 191
Alw21I GWGCWC 1 cut(s) 219
AlwI GGATC 1 cut(s) 308
ApeKI GCWGC 2 cut(s) 290, 293
AspLEI GCGC 1 cut(s) 272
AsuNHI GCTAGC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 277, 280
BccI CCATC 2 cut(s) 13, 121
BfaI CTAG 2 cut(s) 9, 29
BfmI CTRYAG 1 cut(s) 166
BglII AGATCT 1 cut(s) 62
BisI GCNGC 2 cut(s) 291, 294
BlsI GCNGC 2 cut(s) 292, 295
BmtI GCTAGC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 216
BseGI GGATG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 277, 280
BsgI GTGCAG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 2 cut(s) 62, 313
BspACI CCGC 2 cut(s) 95, 113
BspMAI CTGCAG 1 cut(s) 170
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 109
BsrI ACTGG 1 cut(s) 216
BssAI RCCGGY 1 cut(s) 109
BssMI GATC 2 cut(s) 62, 313
Bst4CI ACNGT 1 cut(s) 75
BstC8I GCNNGC 2 cut(s) 30, 193
BstF5I GGATG 1 cut(s) 103
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 2 cut(s) 65, 316
BstMBI GATC 2 cut(s) 62, 313
BstMWI GCNNNNNNNGC 2 cut(s) 34, 230
BstNSI RCATGY 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 277, 280
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BtsCI GGATG 1 cut(s) 103
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 2 cut(s) 209, 303
Cac8I GCNNGC 2 cut(s) 30, 193
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 1 cut(s) 109
CviAII CATG 2 cut(s) 221, 276
CviJI RGCY 5 cut(s) 22, 32, 121, 143, 191
CviKI_1 RGCY 5 cut(s) 22, 32, 121, 143, 191
DpnI GATC 2 cut(s) 64, 315
DpnII GATC 2 cut(s) 62, 313
EciI GGCGGA 1 cut(s) 128
FaeI CATG 2 cut(s) 224, 279
FaiI YATR 6 cut(s) 92, 222, 231, 277, 324, 345
FatI CATG 2 cut(s) 220, 275
Fnu4HI GCNGC 2 cut(s) 291, 294
FokI GGATG 1 cut(s) 110
Fsp4HI GCNGC 2 cut(s) 291, 294
FspBI CTAG 2 cut(s) 9, 29
GlaI GCGC 1 cut(s) 271
GluI GCNGC 2 cut(s) 291, 294
GsuI CTGGAG 1 cut(s) 233
HapII CCGG 1 cut(s) 110
HhaI GCGC 1 cut(s) 272
Hin1II CATG 2 cut(s) 224, 279
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 189
HpaII CCGG 1 cut(s) 110
Hpy166II GTNNAC 1 cut(s) 71
Hpy8I GTNNAC 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 145, 260
HpyCH4III ACNGT 1 cut(s) 75
HpyCH4V TGCA 5 cut(s) 90, 161, 168, 296, 333
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 230
Hsp92II CATG 2 cut(s) 224, 279
HspAI GCGC 1 cut(s) 270
Kzo9I GATC 2 cut(s) 62, 313
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 4 cut(s) 51, 123, 197, 248
Lsp1109I GCAGC 2 cut(s) 277, 280
MaeI CTAG 2 cut(s) 9, 29
MaeIII GTNAC 3 cut(s) 49, 278, 301
MalI GATC 2 cut(s) 64, 315
MboI GATC 2 cut(s) 62, 313
MboII GAAGA 1 cut(s) 72
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 1 cut(s) 241
MnlI CCTC 3 cut(s) 73, 149, 235
MseI TTAA 1 cut(s) 352
MspI CCGG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 34, 230
NdeII GATC 2 cut(s) 62, 313
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 2 cut(s) 224, 279
NmuCI GTSAC 1 cut(s) 301
NspI RCATGY 1 cut(s) 279
PciI ACATGT 1 cut(s) 275
PkrI GCNGC 2 cut(s) 292, 295
PscI ACATGT 1 cut(s) 275
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 62
SaqAI TTAA 1 cut(s) 352
SatI GCNGC 2 cut(s) 291, 294
Sau3AI GATC 2 cut(s) 62, 313
SduI GDGCHC 1 cut(s) 219
SetI ASST 4 cut(s) 34, 123, 160, 193
SfcI CTRYAG 1 cut(s) 166
SgrAI CRCCGGYG 1 cut(s) 109
Sse9I AATT 1 cut(s) 241
SsiI CCGC 2 cut(s) 95, 113
SspMI CTAG 2 cut(s) 9, 29
TaaI ACNGT 1 cut(s) 75
TasI AATT 1 cut(s) 241
Tru1I TTAA 1 cut(s) 352
Tru9I TTAA 1 cut(s) 352
TscAI CASTG 2 cut(s) 216, 310
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 2 cut(s) 290, 293
Tsp45I GTSAC 1 cut(s) 301
TspRI CASTG 2 cut(s) 216, 310
XceI RCATGY 1 cut(s) 279
XspI CTAG 2 cut(s) 9, 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.