Rh5BG008300

Glucan endo-13-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011532
Physical Location & Seq
Forward (+)
552738 .. 554299
1562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG008300.1

Sequence Viewer

Length: 348 bp
ATGCCATCAAGATTTCTATGGCTTCTGCTAGCTCTGCTGTGTTTAGCCCTTACACCACGAAGATCTGGTGGACAGTTAGAGGAATGGTGCATAGCTGATGAGCAGACGCCGGCGGACGAGCTAAAGATGGCACTTGATTGGGCTTGTGAGAAGGGAGGTGCAGACTGCAGTAAGATACAAGAGAACCAAGCTTGCTACTTGCCTAATACACTGGAGCACCATGCCTCTTATGCTTTCAACAATTACTATCAAAAGTTCAAGCAGGAAGGTGCAACATGTTACTTCAATGCTGCTGCAATTGTCACTGCCCTTAATCCAAGTAACATAATTCTGAAGATACTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
X8

Protein Analysis

115

Amino Acids

12.96

Weight (kDa)

5.26

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 29 - 99 1.4e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 113
AcyI GRCGYC 1 cut(s) 107
AflIII ACRYGT 1 cut(s) 275
AgsI TTSAA 3 cut(s) 238, 259, 286
AluBI AGCT 4 cut(s) 32, 95, 121, 191
AluI AGCT 4 cut(s) 32, 95, 121, 191
Alw21I GWGCWC 1 cut(s) 219
ApeKI GCWGC 2 cut(s) 290, 293
AsuNHI GCTAGC 1 cut(s) 28
BarI GAAGNNNNNNTAC 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 277, 280
BccI CCATC 2 cut(s) 13, 121
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 166
BglII AGATCT 1 cut(s) 62
BisI GCNGC 2 cut(s) 291, 294
BlsI GCNGC 2 cut(s) 292, 295
BmtI GCTAGC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 233
BsaHI GRCGYC 1 cut(s) 107
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 277, 280
BsgI GTGCAG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 1 cut(s) 62
BspACI CCGC 1 cut(s) 113
BspMAI CTGCAG 1 cut(s) 170
BspOI GCTAGC 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 109
BsrI ACTGG 1 cut(s) 216
BssAI RCCGGY 1 cut(s) 109
BssMI GATC 1 cut(s) 62
BssNI GRCGYC 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 75
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 3 cut(s) 30, 111, 193
BstKTI GATC 1 cut(s) 65
BstMBI GATC 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 34, 230
BstNSI RCATGY 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 277, 280
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 2 cut(s) 209, 303
Cac8I GCNNGC 3 cut(s) 30, 111, 193
Cfr10I RCCGGY 1 cut(s) 109
CseI GACGC 1 cut(s) 115
CviAII CATG 2 cut(s) 221, 276
CviJI RGCY 7 cut(s) 22, 32, 47, 95, 121, 143, 191
CviKI_1 RGCY 7 cut(s) 22, 32, 47, 95, 121, 143, 191
DpnI GATC 1 cut(s) 64
DpnII GATC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 128
FaeI CATG 2 cut(s) 224, 279
FaiI YATR 6 cut(s) 19, 92, 222, 231, 277, 326
FatI CATG 2 cut(s) 220, 275
Fnu4HI GCNGC 2 cut(s) 291, 294
Fsp4HI GCNGC 2 cut(s) 291, 294
FspBI CTAG 1 cut(s) 29
GluI GCNGC 2 cut(s) 291, 294
GsuI CTGGAG 1 cut(s) 233
HapII CCGG 1 cut(s) 110
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 2 cut(s) 224, 279
HindIII AAGCTT 1 cut(s) 189
HpaII CCGG 1 cut(s) 110
Hpy166II GTNNAC 1 cut(s) 71
Hpy188I TCNGA 1 cut(s) 333
Hpy188III TCNNGA 1 cut(s) 9
Hpy8I GTNNAC 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 145, 260
HpyCH4III ACNGT 1 cut(s) 75
HpyCH4V TGCA 5 cut(s) 90, 161, 168, 272, 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 230
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 2 cut(s) 224, 279
KroI GCCGGC 1 cut(s) 109
KroNI GCCGGC 1 cut(s) 111
Kzo9I GATC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 4 cut(s) 51, 123, 197, 248
Lsp1109I GCAGC 2 cut(s) 277, 280
MaeI CTAG 1 cut(s) 29
MaeIII GTNAC 3 cut(s) 278, 301, 320
MalI GATC 1 cut(s) 64
MboI GATC 1 cut(s) 62
MboII GAAGA 2 cut(s) 72, 346
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 3 cut(s) 241, 297, 327
MnlI CCTC 3 cut(s) 73, 149, 235
MreI CGCCGGCG 1 cut(s) 109
MroNI GCCGGC 1 cut(s) 109
MseI TTAA 1 cut(s) 312
MspI CCGG 1 cut(s) 110
MunI CAATTG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 34, 230
NaeI GCCGGC 1 cut(s) 111
NdeII GATC 1 cut(s) 62
NgoMIV GCCGGC 1 cut(s) 109
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 2 cut(s) 224, 279
NmuCI GTSAC 1 cut(s) 301
NspI RCATGY 1 cut(s) 279
PciI ACATGT 1 cut(s) 275
PdiI GCCGGC 1 cut(s) 111
PkrI GCNGC 2 cut(s) 292, 295
PscI ACATGT 1 cut(s) 275
PstI CTGCAG 1 cut(s) 170
PsuI RGATCY 1 cut(s) 62
SaqAI TTAA 1 cut(s) 312
SatI GCNGC 2 cut(s) 291, 294
Sau3AI GATC 1 cut(s) 62
SduI GDGCHC 1 cut(s) 219
SetI ASST 6 cut(s) 34, 97, 123, 160, 193, 271
SfcI CTRYAG 1 cut(s) 166
SgrAI CRCCGGYG 1 cut(s) 109
Sse9I AATT 3 cut(s) 241, 297, 327
SsiI CCGC 1 cut(s) 113
SspMI CTAG 1 cut(s) 29
TaaI ACNGT 1 cut(s) 75
TasI AATT 3 cut(s) 241, 297, 327
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
TscAI CASTG 2 cut(s) 216, 310
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 2 cut(s) 290, 293
Tsp45I GTSAC 1 cut(s) 301
TspRI CASTG 2 cut(s) 216, 310
XceI RCATGY 1 cut(s) 279
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.