MD00G1058100.v1.1

Glucan endo-13-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0011532
Physical Location & Seq
Forward (+)
10634861 .. 10636238
1378 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1058100.v1.1.491

Sequence Viewer

Length: 357 bp
ATGTCTTCTACTTTCCTTTGGGTTCTGCTACCTTTCCTATTTTTGGCTCTAACTCCACAAAGATCTGGTGGGCAGCTAGATCAGGAATGGTGCATAGCAGATGAGCAGACCCCGGATGAGGAGCTGCAAATGGCAATGAAGTGGGCGTGTGAAGCGGGAGGTGCAGACTGCAGCAAGATACAAGAAAACCAAGAATGCTTCTGGCCTAATACTACGCAGCACCATGCCTCTTATGCCTTCAACAATTACTATCAAAGATTCAAGCAGCAAGGAGCAACTTGCTACTTCAATTCTGCCGCATTGGTCACTGCCCTTGATCCAAGTGATAATTCGTGCAAGTTTGAGTATCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
X8

Protein Analysis

119

Amino Acids

13.48

Weight (kDa)

4.42

Isoelectric Point (pI)

50.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 30 - 100 7.1e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 155, 297
AclWI GGATC 1 cut(s) 311
AfiI CCNNNNNNNGG 2 cut(s) 43, 118
AgsI TTSAA 3 cut(s) 241, 262, 289
AjuI GAANNNNNNNTTGG 2 cut(s) 313, 345
AluBI AGCT 2 cut(s) 76, 124
AluI AGCT 2 cut(s) 76, 124
AlwI GGATC 1 cut(s) 311
AoxI GGCC 1 cut(s) 203
ApeKI GCWGC 5 cut(s) 73, 124, 171, 217, 265
AsuC2I CCSGG 1 cut(s) 113
BbvI GCAGC 5 cut(s) 85, 111, 183, 229, 277
BcnI CCSGG 1 cut(s) 113
BfaI CTAG 1 cut(s) 77
BfmI CTRYAG 1 cut(s) 169
BglII AGATCT 1 cut(s) 62
BisI GCNGC 6 cut(s) 74, 125, 172, 218, 266, 297
BlsI GCNGC 6 cut(s) 75, 126, 173, 219, 267, 298
Bme1390I CCNGG 1 cut(s) 113
BmrFI CCNGG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 118
Bse3DI GCAATG 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 2 cut(s) 43, 118
BseMI GCAATG 1 cut(s) 141
BseRI GAGGAG 1 cut(s) 134
BseXI GCAGC 5 cut(s) 85, 111, 183, 229, 277
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 1 cut(s) 205
BsiSI CCGG 1 cut(s) 113
BslI CCNNNNNNNGG 2 cut(s) 43, 118
BsmI GAATGC 1 cut(s) 200
BsnI GGCC 1 cut(s) 205
Bsp143I GATC 3 cut(s) 62, 79, 316
BspACI CCGC 2 cut(s) 155, 297
BspANI GGCC 1 cut(s) 205
BspMAI CTGCAG 1 cut(s) 173
BspPI GGATC 1 cut(s) 311
BsrDI GCAATG 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 3 cut(s) 62, 79, 316
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 3 cut(s) 65, 82, 319
BstMBI GATC 3 cut(s) 62, 79, 316
BstMWI GCNNNNNNNGC 3 cut(s) 152, 161, 233
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 1 cut(s) 169
BstV1I GCAGC 5 cut(s) 85, 111, 183, 229, 277
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BsuRI GGCC 1 cut(s) 205
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 306
CviAII CATG 1 cut(s) 224
CviJI RGCY 4 cut(s) 47, 76, 124, 205
CviKI_1 RGCY 4 cut(s) 47, 76, 124, 205
DpnI GATC 3 cut(s) 64, 81, 318
DpnII GATC 3 cut(s) 62, 79, 316
FaeI CATG 1 cut(s) 227
FaiI YATR 3 cut(s) 95, 225, 234
FatI CATG 1 cut(s) 223
FauI CCCGC 1 cut(s) 148
Fnu4HI GCNGC 6 cut(s) 74, 125, 172, 218, 266, 297
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 6 cut(s) 74, 125, 172, 218, 266, 297
FspBI CTAG 1 cut(s) 77
GluI GCNGC 6 cut(s) 74, 125, 172, 218, 266, 297
HaeIII GGCC 1 cut(s) 205
HapII CCGG 1 cut(s) 113
Hin1II CATG 1 cut(s) 227
HinfI GANTC 1 cut(s) 258
HpaII CCGG 1 cut(s) 113
Hpy188III TCNNGA 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 247
HpyCH4V TGCA 5 cut(s) 93, 127, 164, 171, 336
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 161, 233
Hsp92II CATG 1 cut(s) 227
Kzo9I GATC 3 cut(s) 62, 79, 316
LmnI GCTCC 2 cut(s) 121, 272
LpnPI CCDG 4 cut(s) 51, 68, 126, 187
Lsp1109I GCAGC 5 cut(s) 85, 111, 183, 229, 277
MaeI CTAG 1 cut(s) 77
MaeIII GTNAC 1 cut(s) 304
MalI GATC 3 cut(s) 64, 81, 318
MboI GATC 3 cut(s) 62, 79, 316
MboII GAAGA 1 cut(s) 341
MflI RGATCY 1 cut(s) 62
MluCI AATT 3 cut(s) 244, 289, 328
MnlI CCTC 3 cut(s) 112, 152, 238
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 152, 161, 233
NciI CCSGG 1 cut(s) 113
NdeII GATC 3 cut(s) 62, 79, 316
NlaIII CATG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 304
PctI GAATGC 1 cut(s) 200
PfeI GAWTC 1 cut(s) 258
PkrI GCNGC 6 cut(s) 75, 126, 173, 219, 267, 298
PstI CTGCAG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 62
SatI GCNGC 6 cut(s) 74, 125, 172, 218, 266, 297
Sau3AI GATC 3 cut(s) 62, 79, 316
ScrFI CCNGG 1 cut(s) 113
SetI ASST 4 cut(s) 34, 78, 126, 163
SfcI CTRYAG 1 cut(s) 169
Sse9I AATT 3 cut(s) 244, 289, 328
SsiI CCGC 2 cut(s) 155, 297
SspMI CTAG 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 111
TasI AATT 3 cut(s) 244, 289, 328
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 1 cut(s) 258
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 5 cut(s) 73, 124, 171, 217, 265
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 152
TspRI CASTG 1 cut(s) 313
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.