MD10G1336600.v1.1

PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011532
Physical Location & Seq
Reverse (-)
41298472 .. 41299903
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1336600.v1.1.491

Sequence Viewer

Length: 357 bp
ATGTCTTCTATTTTCCTTTATGTTCTGCTAGCTCTGTTATTTTTGTCTCTAACTCCACAAAGATCTGATGGGCAGCTAGATCCGGAATGGTGCATAGCAGATGAGCAGACACCAGATGAGGATCTGCAAATGGCGATGAAGTGGGCTTGTGAAGTGGGAGGTGCCGACTGCAGCAAGATACAAGAAAACCAAGACTGCTACTTACCAAATACCATGCAGGACCACGCCTCTTATGTCTTCAACAATTACTACCAAAGATTTAAGAAGCAAGGAGGAACTTGCTACTTCAATTCTGCTGCATTGGTCACTGCCCTTGATCCAAGTCATAATTCGTGCAAGTTTGAGTATCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
X8

Protein Analysis

119

Amino Acids

13.44

Weight (kDa)

4.4

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 30 - 100 1.2e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccIII TCCGGA 1 cut(s) 82
AclWI GGATC 3 cut(s) 74, 129, 311
AgsI TTSAA 2 cut(s) 241, 289
AjuI GAANNNNNNNTTGG 2 cut(s) 313, 345
AluBI AGCT 2 cut(s) 32, 76
AluI AGCT 2 cut(s) 32, 76
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 3 cut(s) 74, 129, 311
Aor13HI TCCGGA 1 cut(s) 82
ApeKI GCWGC 3 cut(s) 73, 171, 296
AspS9I GGNCC 1 cut(s) 220
AsuNHI GCTAGC 1 cut(s) 28
AvaII GGWCC 1 cut(s) 220
BanI GGYRCC 1 cut(s) 161
BbsI GAAGAC 1 cut(s) 229
BbvI GCAGC 3 cut(s) 85, 183, 283
BccI CCATC 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 2 cut(s) 29, 77
BfmI CTRYAG 1 cut(s) 169
BglII AGATCT 1 cut(s) 62
BisI GCNGC 3 cut(s) 74, 172, 297
BlsI GCNGC 3 cut(s) 75, 173, 298
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 163
BmtI GCTAGC 1 cut(s) 32
BpiI GAAGAC 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 120
BsaWI WCCGGW 1 cut(s) 82
Bse8I GATNNNNATC 1 cut(s) 120
BseAI TCCGGA 1 cut(s) 82
BseJI GATNNNNATC 1 cut(s) 120
BseXI GCAGC 3 cut(s) 85, 183, 283
BshNI GGYRCC 1 cut(s) 161
BsiSI CCGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 51
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 4 cut(s) 62, 79, 121, 316
BspEI TCCGGA 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 173
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 3 cut(s) 74, 129, 311
BspT107I GGYRCC 1 cut(s) 161
BssMI GATC 4 cut(s) 62, 79, 121, 316
BstC8I GCNNGC 1 cut(s) 30
BstKTI GATC 4 cut(s) 65, 82, 124, 319
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 4 cut(s) 62, 79, 121, 316
BstSFI CTRYAG 1 cut(s) 169
BstV1I GCAGC 3 cut(s) 85, 183, 283
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 3 cut(s) 62, 79, 121
BstYI RGATCY 3 cut(s) 62, 79, 121
BtgZI GCGATG 1 cut(s) 149
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 220
CviAII CATG 1 cut(s) 214
CviJI RGCY 3 cut(s) 32, 76, 146
CviKI_1 RGCY 3 cut(s) 32, 76, 146
DpnI GATC 4 cut(s) 64, 81, 123, 318
DpnII GATC 4 cut(s) 62, 79, 121, 316
Eco47I GGWCC 1 cut(s) 220
FaeI CATG 1 cut(s) 217
FaiI YATR 5 cut(s) 21, 95, 215, 234, 327
FatI CATG 1 cut(s) 213
Fnu4HI GCNGC 3 cut(s) 74, 172, 297
Fsp4HI GCNGC 3 cut(s) 74, 172, 297
FspBI CTAG 2 cut(s) 29, 77
GluI GCNGC 3 cut(s) 74, 172, 297
HapII CCGG 1 cut(s) 83
Hin1II CATG 1 cut(s) 217
HpaII CCGG 1 cut(s) 83
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 83
HpyCH4V TGCA 6 cut(s) 93, 127, 171, 217, 299, 336
Hsp92II CATG 1 cut(s) 217
Kpn2I TCCGGA 1 cut(s) 82
Kzo9I GATC 4 cut(s) 62, 79, 121, 316
LpnPI CCDG 3 cut(s) 96, 126, 203
Lsp1109I GCAGC 3 cut(s) 85, 183, 283
MaeI CTAG 2 cut(s) 29, 77
MaeIII GTNAC 1 cut(s) 304
MalI GATC 4 cut(s) 64, 81, 123, 318
MboI GATC 4 cut(s) 62, 79, 121, 316
MboII GAAGA 2 cut(s) 229, 341
MflI RGATCY 3 cut(s) 62, 79, 121
MluCI AATT 3 cut(s) 244, 289, 328
MnlI CCTC 4 cut(s) 112, 152, 238, 266
MroI TCCGGA 1 cut(s) 82
MseI TTAA 1 cut(s) 261
MspI CCGG 1 cut(s) 83
NdeII GATC 4 cut(s) 62, 79, 121, 316
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 1 cut(s) 217
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 304
PkrI GCNGC 3 cut(s) 75, 173, 298
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 220
PstI CTGCAG 1 cut(s) 173
PsuI RGATCY 3 cut(s) 62, 79, 121
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 3 cut(s) 74, 172, 297
Sau3AI GATC 4 cut(s) 62, 79, 121, 316
Sau96I GGNCC 1 cut(s) 220
SetI ASST 3 cut(s) 34, 78, 163
SfcI CTRYAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 220
Sse9I AATT 3 cut(s) 244, 289, 328
SspMI CTAG 2 cut(s) 29, 77
TasI AATT 3 cut(s) 244, 289, 328
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 3 cut(s) 73, 171, 296
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 1 cut(s) 152
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 220
XspI CTAG 2 cut(s) 29, 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.