Rw5G000660

Glucan endo-13-beta-glucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011532
Physical Location & Seq
Forward (+)
446071 .. 446613
543 bp
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UTR
Exon/CDS
Intron
Rw5G000660.1

Sequence Viewer

Length: 348 bp
ATGCCATCAAGATTTCTATGGCTTCTGCTAGCTCTGCTGTGTTTAGCCCTAACACCACGAAGATCTGTTAGAGAGGAATGGTGCATAGCTGATAAGCAGACCCCGGCGGACGAGCTAAAGATGGCACTTGATTGGGCTTGTGAGAAGGGAGGTGCAGACTGCAGTAAGATACAAGAGAACCAAGCTTGCTACTTGCCTAATACTCTGGAGCACCATGCCTCTTATGCTTTCAACAATTACTATCAAAAGTTCAAGCAGGAAGGTGCAACATGTTACTTCAATGCTGCTGCATTTGTCACTGCCCTTGATCCAAGTCATAACCCTTGCAAGTTTGAGTATCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
X8

Protein Analysis

115

Amino Acids

13.16

Weight (kDa)

5.86

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 27 - 97 2.4e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 107
AclWI GGATC 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 269
AgsI TTSAA 3 cut(s) 232, 253, 280
AluBI AGCT 4 cut(s) 32, 89, 115, 185
AluI AGCT 4 cut(s) 32, 89, 115, 185
Alw21I GWGCWC 1 cut(s) 213
AlwI GGATC 1 cut(s) 302
ApeKI GCWGC 2 cut(s) 284, 287
AsuC2I CCSGG 1 cut(s) 104
AsuNHI GCTAGC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 271, 274
BccI CCATC 2 cut(s) 13, 115
BcnI CCSGG 1 cut(s) 104
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 160
BglII AGATCT 1 cut(s) 62
BisI GCNGC 2 cut(s) 285, 288
BlsI GCNGC 2 cut(s) 286, 289
Bme1390I CCNGG 1 cut(s) 104
BmrFI CCNGG 1 cut(s) 104
BmtI GCTAGC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 271, 274
BsgI GTGCAG 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 213
BsiSI CCGG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 62, 307
BspACI CCGC 1 cut(s) 107
BspMAI CTGCAG 1 cut(s) 164
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 2 cut(s) 62, 307
BstC8I GCNNGC 2 cut(s) 30, 187
BstKTI GATC 2 cut(s) 65, 310
BstMBI GATC 2 cut(s) 62, 307
BstMWI GCNNNNNNNGC 2 cut(s) 34, 224
BstNSI RCATGY 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 160
BstV1I GCAGC 2 cut(s) 271, 274
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 2 cut(s) 30, 187
CviAII CATG 2 cut(s) 215, 270
CviJI RGCY 7 cut(s) 22, 32, 47, 89, 115, 137, 185
CviKI_1 RGCY 7 cut(s) 22, 32, 47, 89, 115, 137, 185
DpnI GATC 2 cut(s) 64, 309
DpnII GATC 2 cut(s) 62, 307
EciI GGCGGA 1 cut(s) 122
FaeI CATG 2 cut(s) 218, 273
FaiI YATR 6 cut(s) 19, 86, 216, 225, 271, 318
FatI CATG 2 cut(s) 214, 269
Fnu4HI GCNGC 2 cut(s) 285, 288
Fsp4HI GCNGC 2 cut(s) 285, 288
FspBI CTAG 1 cut(s) 29
GluI GCNGC 2 cut(s) 285, 288
GsuI CTGGAG 1 cut(s) 227
HapII CCGG 1 cut(s) 104
Hin1II CATG 2 cut(s) 218, 273
HindIII AAGCTT 1 cut(s) 183
HpaII CCGG 1 cut(s) 104
Hpy188III TCNNGA 2 cut(s) 9, 206
HpyAV CCTTC 2 cut(s) 139, 254
HpyCH4V TGCA 6 cut(s) 84, 155, 162, 266, 290, 327
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 224
Hsp92II CATG 2 cut(s) 218, 273
Kzo9I GATC 2 cut(s) 62, 307
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 3 cut(s) 117, 191, 242
Lsp1109I GCAGC 2 cut(s) 271, 274
MaeI CTAG 1 cut(s) 29
MaeIII GTNAC 2 cut(s) 272, 295
MalI GATC 2 cut(s) 64, 309
MboI GATC 2 cut(s) 62, 307
MboII GAAGA 2 cut(s) 72, 332
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 1 cut(s) 235
MnlI CCTC 3 cut(s) 67, 143, 229
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 34, 224
NciI CCSGG 1 cut(s) 104
NdeII GATC 2 cut(s) 62, 307
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 2 cut(s) 218, 273
NmuCI GTSAC 1 cut(s) 295
NspI RCATGY 1 cut(s) 273
PciI ACATGT 1 cut(s) 269
PkrI GCNGC 2 cut(s) 286, 289
PscI ACATGT 1 cut(s) 269
PstI CTGCAG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 62
SatI GCNGC 2 cut(s) 285, 288
Sau3AI GATC 2 cut(s) 62, 307
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 1 cut(s) 213
SetI ASST 6 cut(s) 34, 91, 117, 154, 187, 265
SfcI CTRYAG 1 cut(s) 160
Sse9I AATT 1 cut(s) 235
SsiI CCGC 1 cut(s) 107
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 102
TasI AATT 1 cut(s) 235
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 2 cut(s) 284, 287
Tsp45I GTSAC 1 cut(s) 295
TspRI CASTG 1 cut(s) 304
XceI RCATGY 1 cut(s) 273
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.