FvH4_3g00780

Auxin-induced in root cultures protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012068
Physical Location & Seq
Forward (+)
390818 .. 393138
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00780.t1

Sequence Viewer

Length: 1179 bp
ATGGCAGCCATGCTCAAGCTTCTCATAGCTTTCTGTGCTTTGGTCTCTCTATCTTCCTTCTCAGATGCCCAAACTACATGTTCCAGCTACAATAAGTTCACCAGCAACCGAGTCTTTACTTCCTGCAGCGACCTCCCGTCCCTCAACGCCTTCCTCCACTGGACCTTCAACCAGTCCACCGGGAATCTCAAGATGGCGTACAGGCACACGCCATCCACATCCACCAACTGGGTCGCCTGGGCCATCAACCCGGACCTCACCAAGTCCCAGAAGGCCATGATCGGCGCCCAAGCTCTTGTTGCTATTCCTCAAACCAGCGGGGCTCCCTCACTCCGCATGTCTTCGATCACCGGATACGACACGTCGTTGGCCAACGGGAGCTTGAGTTACGTTGTGACCGACTTGTCGGGTGAGGTTCAGAGCGGAGAGGTGATCATCTACGCCACTTGGACCCTGCCGACGAATACCACGTCGATTCTTCAGGTTTGGCAGGAGGGTCCGGTTGCGAGTGGGACCCCACAAGCACACGCAATTGCTGTGGCTAATCTTAATGCTCAGGGGACTTTGGACTTGCTTTCCGGCACGACTGCCTCTTCCGGTGGAGCCAGCTCCACACTCAAGAAGAAAAACATTCATGCAGTGCTAAATGTTGTGAGTTGGGGTATCTTGATGCCTTTTGGAGCCATGGTAGCAAGGTACTTGAAGACATTCAAGGCTGCAGACCCGGCATGGTTTTACCTCCACGCCGGTTGCCAGACCTCTGCCTATATTGTTGGTGTTGCTGGATGGGCAACTGGTATTAAACTCGGTAGCGATTCTGCTGGTATCCAATATGACACTCACAGAAACATTGGGATTACTCTCTTCTGCATTGGAACACTTCAGGTGTTTGCTTTGCTTCTGAGACCCAACAAGGATCACAAGTTCAGATTCTACTGGAATATCTACCATCATGTGACAGGGTATCTTGTCATCACTCTCAGCATTGTCAACATATTCGAAGGATTTGACATCTTGAAACCAGACAAGAAGTGGAAGAATTCATACATTGGCCTCATCATTGCTTTGGCTGTCCAAGCTGCTTTGTTGGAAGCTTACACATGGTATGTTGTAATGAAGAGGAAGAAGGCTGAAAAGGCGCCCCACGGCGTGAATGGTGTCAACGGCTATGGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

393

Amino Acids

42.39

Weight (kDa)

9.33

Isoelectric Point (pI)

26.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 93 - 191 9.9e-24 Protein of unknown function (DUF568)
Cytochrom_B561 PF03188 211 - 336 1.5e-07 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17280 AT5G47530
fragaria_vesca FvH4_3g00780 FvH4_3g00780
malus_domestica MD05G1359500.v1.1 MD10G1335000.v1.1
prunus_persica Prupe.4G008600_v2.0.a1
pyrus_communis pycom10g28480
rosa_chinensis RchiOBHm_Chr5g0001021
rosa_laevigata RLG00000030914
rosa_multiflora Rmu_sc0000255.1_g000030 Rmu_sc0004189.1_g000001
rosa_roxburghii Rroxscaffold_1G00075260
rosa_rugosa Rorug04G0389000
rosa_samantha Rh5AG008200 Rh5BG010300 Rh5CG008900 Rh5DG009000
rosa_wichuraiana Rw5G000840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 403
AccB1I GGYRCC 2 cut(s) 284, 1138
AccB7I CCANNNNNTGG 1 cut(s) 228
AccBSI CCGCTC 1 cut(s) 423
AciI CCGC 3 cut(s) 318, 334, 423
AclWI GGATC 1 cut(s) 924
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 1 cut(s) 1039
AcuI CTGAAG 2 cut(s) 464, 866
AcyI GRCGYC 2 cut(s) 285, 1139
AdeI CACNNNGTG 1 cut(s) 1150
AfaI GTAC 2 cut(s) 200, 698
AfiI CCNNNNNNNGG 1 cut(s) 228
AflIII ACRYGT 2 cut(s) 77, 360
AgsI TTSAA 4 cut(s) 169, 703, 712, 1018
AhdI GACNNNNNGTC 1 cut(s) 136
AjiI CACGTC 2 cut(s) 363, 471
AjnI CCWGG 1 cut(s) 236
AloI GAACNNNNNNTCC 2 cut(s) 908, 940
AluBI AGCT 8 cut(s) 19, 29, 87, 293, 381, 609, 1079, 1094
AluI AGCT 8 cut(s) 19, 29, 87, 293, 381, 609, 1079, 1094
Alw26I GTCTC 2 cut(s) 49, 898
AlwI GGATC 1 cut(s) 924
AoxI GGCC 4 cut(s) 240, 273, 369, 1051
ApeKI GCWGC 4 cut(s) 5, 126, 716, 1079
ApoI RAATTY 1 cut(s) 1039
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
Asp700I GAANNNNTTC 1 cut(s) 707
AspLEI GCGC 2 cut(s) 287, 1141
AspS9I GGNCC 6 cut(s) 162, 240, 253, 450, 497, 513
AsuC2I CCSGG 3 cut(s) 181, 251, 725
AsuHPI GGTGA 5 cut(s) 91, 250, 340, 422, 442
AsuII TTCGAA 1 cut(s) 999
AvaII GGWCC 5 cut(s) 162, 253, 450, 497, 513
BalI TGGCCA 1 cut(s) 371
BanI GGYRCC 2 cut(s) 284, 1138
BanII GRGCYC 1 cut(s) 325
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
BbsI GAAGAC 2 cut(s) 333, 710
BbvI GCAGC 4 cut(s) 17, 138, 703, 1066
BccI CCATC 5 cut(s) 187, 220, 251, 780, 957
BceAI ACGGC 1 cut(s) 1162
BciT130I CCWGG 1 cut(s) 238
BciVI GTATCC 2 cut(s) 347, 836
BclI TGATCA 1 cut(s) 432
BcnI CCSGG 3 cut(s) 181, 251, 725
BcoDI GTCTC 2 cut(s) 49, 898
BfmI CTRYAG 2 cut(s) 124, 717
BfoI RGCGCY 2 cut(s) 288, 1142
BfuI GTATCC 2 cut(s) 347, 836
BisI GCNGC 4 cut(s) 6, 127, 717, 1080
BlsI GCNGC 4 cut(s) 7, 128, 718, 1081
Bme1390I CCNGG 4 cut(s) 181, 238, 251, 725
Bme18I GGWCC 5 cut(s) 162, 253, 450, 497, 513
BmeRI GACNNNNNGTC 1 cut(s) 136
BmgBI CACGTC 2 cut(s) 363, 471
BmgT120I GGNCC 6 cut(s) 162, 240, 253, 450, 497, 513
BmiI GGNNCC 9 cut(s) 286, 324, 452, 498, 514, 515, 604, 682, 1140
BmrFI CCNGG 4 cut(s) 181, 238, 251, 725
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 55, 660
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 2 cut(s) 333, 710
Bpu10I CCTNAGC 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 999
BpuEI CTTGAG 3 cut(s) 173, 403, 602
BpuMI CCSGG 3 cut(s) 181, 251, 725
BsaHI GRCGYC 2 cut(s) 285, 1139
BsaI GGTCTC 2 cut(s) 49, 898
BsaJI CCNNGG 3 cut(s) 237, 684, 1144
BsaWI WCCGGW 3 cut(s) 350, 499, 596
BsaXI ACNNNNNCTCC 4 cut(s) 370, 400, 672, 702
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse118I RCCGGY 1 cut(s) 746
Bse1I ACTGG 5 cut(s) 164, 172, 233, 799, 941
Bse3DI GCAATG 1 cut(s) 1059
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 3 cut(s) 237, 684, 1144
BseGI GGATG 3 cut(s) 212, 218, 791
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMI GCAATG 1 cut(s) 1059
BseMII CTCAG 4 cut(s) 75, 569, 893, 994
BseNI ACTGG 5 cut(s) 164, 172, 233, 799, 941
BseXI GCAGC 4 cut(s) 17, 138, 703, 1066
BshFI GGCC 4 cut(s) 242, 275, 371, 1053
BshNI GGYRCC 2 cut(s) 284, 1138
BsiSI CCGG 8 cut(s) 180, 251, 351, 500, 579, 597, 725, 747
BslFI GGGAC 4 cut(s) 124, 250, 526, 574
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 2 cut(s) 49, 898
BsmFI GGGAC 4 cut(s) 124, 250, 526, 574
BsnI GGCC 4 cut(s) 242, 275, 371, 1053
Bso31I GGTCTC 2 cut(s) 49, 898
Bsp119I TTCGAA 1 cut(s) 999
Bsp1286I GDGCHC 1 cut(s) 325
Bsp143I GATC 4 cut(s) 279, 345, 432, 916
Bsp19I CCATGG 1 cut(s) 684
BspACI CCGC 3 cut(s) 318, 334, 423
BspANI GGCC 4 cut(s) 242, 275, 371, 1053
BspCNI CTCAG 4 cut(s) 74, 568, 894, 993
BspLI GGNNCC 9 cut(s) 286, 324, 452, 498, 514, 515, 604, 682, 1140
BspMAI CTGCAG 2 cut(s) 128, 721
BspPI GGATC 1 cut(s) 924
BspT104I TTCGAA 1 cut(s) 999
BspT107I GGYRCC 2 cut(s) 284, 1138
BspTNI GGTCTC 2 cut(s) 49, 898
BsrBI CCGCTC 1 cut(s) 423
BsrDI GCAATG 1 cut(s) 1059
BsrFI RCCGGY 1 cut(s) 746
BsrI ACTGG 5 cut(s) 164, 172, 233, 799, 941
BssAI RCCGGY 1 cut(s) 746
BssECI CCNNGG 3 cut(s) 237, 684, 1144
BssMI GATC 4 cut(s) 279, 345, 432, 916
BssNI GRCGYC 2 cut(s) 285, 1139
BssT1I CCWWGG 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 238
Bst6I CTCTTC 3 cut(s) 598, 869, 1112
BstACI GRCGYC 2 cut(s) 285, 1139
BstBI TTCGAA 1 cut(s) 999
BstC8I GCNNGC 1 cut(s) 607
BstDEI CTNAG 5 cut(s) 61, 555, 902, 980, 1176
BstDSI CCRYGG 2 cut(s) 684, 1144
BstF5I GGATG 3 cut(s) 212, 218, 791
BstH2I RGCGCY 2 cut(s) 288, 1142
BstHHI GCGC 2 cut(s) 287, 1141
BstKTI GATC 4 cut(s) 282, 348, 435, 919
BstMAI GTCTC 2 cut(s) 49, 898
BstMBI GATC 4 cut(s) 279, 345, 432, 916
BstMWI GCNNNNNNNGC 7 cut(s) 35, 299, 689, 725, 788, 1076, 1136
BstNI CCWGG 1 cut(s) 238
BstNSI RCATGY 2 cut(s) 81, 340
BstSCI CCNGG 4 cut(s) 179, 236, 249, 723
BstSFI CTRYAG 2 cut(s) 124, 717
BstV1I GCAGC 4 cut(s) 17, 138, 703, 1066
BstV2I GAAGAC 2 cut(s) 333, 710
BsuI GTATCC 2 cut(s) 347, 836
BsuRI GGCC 4 cut(s) 242, 275, 371, 1053
BtgI CCRYGG 2 cut(s) 684, 1144
BtrI CACGTC 2 cut(s) 363, 471
BtsCI GGATG 3 cut(s) 212, 218, 791
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 2 cut(s) 157, 645
Cac8I GCNNGC 1 cut(s) 607
CfoI GCGC 2 cut(s) 287, 1141
Cfr10I RCCGGY 1 cut(s) 746
Cfr13I GGNCC 6 cut(s) 162, 240, 253, 450, 497, 513
Csp6I GTAC 2 cut(s) 199, 697
CspCI CAANNNNNGTGG 4 cut(s) 519, 554, 731, 766
CviAII CATG 9 cut(s) 10, 78, 277, 337, 635, 685, 729, 953, 1101
CviQI GTAC 2 cut(s) 199, 697
DdeI CTNAG 5 cut(s) 61, 555, 902, 980, 1176
DinI GGCGCC 2 cut(s) 286, 1140
DpnI GATC 4 cut(s) 281, 347, 434, 918
DpnII GATC 4 cut(s) 279, 345, 432, 916
DraIII CACNNNGTG 1 cut(s) 1150
DrdI GACNNNNNNGTC 1 cut(s) 403
DriI GACNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 3 cut(s) 598, 869, 1112
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 3 cut(s) 598, 869, 1112
Eco130I CCWWGG 1 cut(s) 684
Eco24I GRGCYC 1 cut(s) 325
Eco31I GGTCTC 2 cut(s) 49, 898
Eco47I GGWCC 5 cut(s) 162, 253, 450, 497, 513
Eco57I CTGAAG 2 cut(s) 464, 866
EcoO109I RGGNCCY 1 cut(s) 513
EcoRI GAATTC 1 cut(s) 1039
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 684
EcoT38I GRGCYC 1 cut(s) 325
EgeI GGCGCC 2 cut(s) 286, 1140
EheI GGCGCC 2 cut(s) 286, 1140
ErhI CCWWGG 1 cut(s) 684
FaeI CATG 9 cut(s) 13, 81, 280, 340, 638, 688, 732, 956, 1104
FaqI GGGAC 4 cut(s) 124, 250, 526, 574
FatI CATG 9 cut(s) 9, 77, 276, 336, 634, 684, 728, 952, 1100
FauI CCCGC 1 cut(s) 311
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 4 cut(s) 6, 127, 717, 1080
FokI GGATG 3 cut(s) 199, 205, 798
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 4 cut(s) 6, 127, 717, 1080
GlaI GCGC 2 cut(s) 286, 1140
GluI GCNGC 4 cut(s) 6, 127, 717, 1080
HaeII RGCGCY 2 cut(s) 288, 1142
HaeIII GGCC 4 cut(s) 242, 275, 371, 1053
HapII CCGG 8 cut(s) 180, 251, 351, 500, 579, 597, 725, 747
HhaI GCGC 2 cut(s) 287, 1141
Hin1I GRCGYC 2 cut(s) 285, 1139
Hin1II CATG 9 cut(s) 13, 81, 280, 340, 638, 688, 732, 956, 1104
Hin6I GCGC 2 cut(s) 285, 1139
HinP1I GCGC 2 cut(s) 285, 1139
HincII GTYRAC 2 cut(s) 991, 1162
HindII GTYRAC 2 cut(s) 991, 1162
HindIII AAGCTT 2 cut(s) 17, 1092
HinfI GANTC 5 cut(s) 111, 184, 475, 815, 930
HpaII CCGG 8 cut(s) 180, 251, 351, 500, 579, 597, 725, 747
HphI GGTGA 5 cut(s) 91, 250, 340, 422, 442
Hpy166II GTNNAC 4 cut(s) 99, 177, 991, 1162
Hpy188I TCNGA 4 cut(s) 64, 420, 903, 929
Hpy188III TCNNGA 4 cut(s) 190, 619, 667, 1015
Hpy8I GTNNAC 4 cut(s) 99, 177, 991, 1162
Hpy99I CGWCG 3 cut(s) 367, 463, 475
HpyAV CCTTC 6 cut(s) 67, 160, 175, 265, 995, 1120
HpyCH4IV ACGT 3 cut(s) 362, 390, 470
HpyCH4V TGCA 4 cut(s) 126, 638, 719, 870
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 299, 689, 725, 788, 1076, 1136
HpyF3I CTNAG 5 cut(s) 61, 555, 902, 980, 1176
HpySE526I ACGT 3 cut(s) 362, 390, 470
Hsp92I GRCGYC 2 cut(s) 285, 1139
Hsp92II CATG 9 cut(s) 13, 81, 280, 340, 638, 688, 732, 956, 1104
HspAI GCGC 2 cut(s) 285, 1139
KasI GGCGCC 2 cut(s) 284, 1138
KflI GGGWCCC 1 cut(s) 513
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 4 cut(s) 279, 345, 432, 916
LmnI GCTCC 5 cut(s) 328, 378, 602, 614, 680
Lsp1109I GCAGC 4 cut(s) 17, 138, 703, 1066
LweI GCATC 2 cut(s) 55, 660
MaeII ACGT 3 cut(s) 362, 390, 470
MaeIII GTNAC 3 cut(s) 386, 394, 955
MalI GATC 4 cut(s) 281, 347, 434, 918
MbiI CCGCTC 1 cut(s) 423
MboI GATC 4 cut(s) 279, 345, 432, 916
MfeI CAATTG 1 cut(s) 531
MhlI GDGCHC 1 cut(s) 325
MlsI TGGCCA 1 cut(s) 371
MluCI AATT 2 cut(s) 531, 1039
MluNI TGGCCA 1 cut(s) 371
Mly113I GGCGCC 2 cut(s) 285, 1139
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 1068
Mox20I TGGCCA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 707
MscI TGGCCA 1 cut(s) 371
MseI TTAA 2 cut(s) 549, 801
Msp20I TGGCCA 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 8 cut(s) 180, 251, 351, 500, 579, 597, 725, 747
MspR9I CCNGG 4 cut(s) 181, 238, 251, 725
MunI CAATTG 1 cut(s) 531
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 7 cut(s) 35, 299, 689, 725, 788, 1076, 1136
NarI GGCGCC 2 cut(s) 285, 1139
NciI CCSGG 3 cut(s) 181, 251, 725
NcoI CCATGG 1 cut(s) 684
NdeII GATC 4 cut(s) 279, 345, 432, 916
NlaIII CATG 9 cut(s) 13, 81, 280, 340, 638, 688, 732, 956, 1104
NlaIV GGNNCC 9 cut(s) 286, 324, 452, 498, 514, 515, 604, 682, 1140
NmuCI GTSAC 2 cut(s) 394, 955
NspI RCATGY 2 cut(s) 81, 340
NspV TTCGAA 1 cut(s) 999
PciI ACATGT 1 cut(s) 77
PcsI WCGNNNNNNNCGW 2 cut(s) 396, 467
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 4 cut(s) 184, 475, 815, 930
PflMI CCANNNNNTGG 1 cut(s) 228
PkrI GCNGC 4 cut(s) 7, 128, 718, 1081
PleI GAGTC 1 cut(s) 119
PluTI GGCGCC 2 cut(s) 288, 1142
PpsI GAGTC 1 cut(s) 119
PpuMI RGGWCCY 1 cut(s) 513
PscI ACATGT 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 513
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 9 cut(s) 286, 324, 452, 498, 514, 515, 604, 682, 1140
PspPI GGNCC 6 cut(s) 162, 240, 253, 450, 497, 513
PspPPI RGGWCCY 1 cut(s) 513
PstI CTGCAG 2 cut(s) 128, 721
RsaI GTAC 2 cut(s) 200, 698
RsaNI GTAC 2 cut(s) 199, 697
SaqAI TTAA 2 cut(s) 549, 801
SatI GCNGC 4 cut(s) 6, 127, 717, 1080
Sau3AI GATC 4 cut(s) 279, 345, 432, 916
Sau96I GGNCC 6 cut(s) 162, 240, 253, 450, 497, 513
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 4 cut(s) 181, 238, 251, 725
SduI GDGCHC 1 cut(s) 325
SfaNI GCATC 2 cut(s) 55, 660
SfcI CTRYAG 2 cut(s) 124, 717
SfoI GGCGCC 2 cut(s) 286, 1140
SfuI TTCGAA 1 cut(s) 999
SinI GGWCC 5 cut(s) 162, 253, 450, 497, 513
SmlI CTYRAG 4 cut(s) 14, 188, 382, 617
SmoI CTYRAG 4 cut(s) 14, 188, 382, 617
Sse9I AATT 2 cut(s) 531, 1039
SsiI CCGC 3 cut(s) 318, 334, 423
SspDI GGCGCC 2 cut(s) 284, 1138
StyD4I CCNGG 4 cut(s) 179, 236, 249, 723
StyI CCWWGG 1 cut(s) 684
TaiI ACGT 3 cut(s) 365, 393, 473
TaqI TCGA 3 cut(s) 344, 473, 999
TaqII GACCGA 1 cut(s) 413
TasI AATT 2 cut(s) 531, 1039
TfiI GAWTC 4 cut(s) 184, 475, 815, 930
Tru1I TTAA 2 cut(s) 549, 801
Tru9I TTAA 2 cut(s) 549, 801
TscAI CASTG 2 cut(s) 164, 645
TseFI GTSAC 2 cut(s) 394, 955
TseI GCWGC 4 cut(s) 5, 126, 716, 1079
Tsp45I GTSAC 2 cut(s) 394, 955
TspDTI ATGAA 3 cut(s) 623, 1032, 1130
TspRI CASTG 2 cut(s) 164, 645
Van91I CCANNNNNTGG 1 cut(s) 228
VpaK11BI GGWCC 5 cut(s) 162, 253, 450, 497, 513
XapI RAATTY 1 cut(s) 1039
XceI RCATGY 2 cut(s) 81, 340
XcmI CCANNNNNNNNNTGG 2 cut(s) 1029, 1151
XmnI GAANNNNTTC 1 cut(s) 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.